Claims
- 1. A kit for performing specific binding assays with an optical unit including a light source positioned to direct light into an optical substrate and detector oriented to detect light from a region proximal to the optical substrate, which includes:
- a biosensor comprising:
- a step-gradient waveguide including a substrate formed of a first optical material of refractive index n.sub.1 and having a surface disposed adjacent and in contact with a waveguide film formed of a second optical material having a refractive index n.sub.2 which is greater than n.sub.1, said substrate surface having a grating formed thereon, said grating constructed to facilitate coupling of light from an incident excitation beam into said waveguide film, said step-gradient waveguide being manufactured by a process of manufacture including the steps of:
- providing a piece of the first optical material having a surface;
- vapor depositing the second optical material on said first optical material surface to a depth of between about 0.1 .mu.m and about 10 .mu.m to produce said waveguide film;
- coating at least one region of said waveguide layer film with a resist compound which resists an etchant to produce a coated region and an uncoated region; and
- etching said waveguide film with said etchant to remove said second optical material from said uncoated region; and
- at least one specific binding molecule immobilized to said waveguide film and constructed to bind with specificity an analyte.
- 2. The kit of claim 1, wherein said second optical material is selected from the group consisting of: silicon oxynitride of refractive index n.sub.2, and deposited silicon dioxide; and said first optical material is selected from the group consisting of: deposited silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n.sub.1, and magnesium fluoride.
- 3. The kit of claim 1, wherein said waveguide film is configured as a plurality of parallel longitudinal strips of said second optical material spaced from one another along said substrate surface and constituting a plurality of waveguide channels, and further having a plurality of different species of said at least one specific binding molecule immobilized to said waveguide film, with each of said waveguide channels having a different one of said species immobilized thereto.
- 4. The kit of claim 1, wherein said waveguide film is coated with a coating which provides a level of nonspecific protein binding which is less than about 10% of an amount of said specific binding of said analyte, said coating being selected from the group consisting of: polymethacryloyl polymer, polyethyleneglycol, and avidin.
- 5. The kit of claim 1, wherein said second optical material is selected from the group consisting of: silicon oxynitride of refractive index n.sub.2, and silicon dioxide; and said first optical material is selected from the group consisting of: deposited silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n.sub.1, and magnesium fluoride.
- 6. The kit of claim 1, in whose manufacture said step of coating at least one region with a resist compound comprises coating a plurality of parallel strips along said first optical material surface with said resist compound, thereby producing a plurality of waveguide channels, and wherein a plurality of different species of said at least one specific binding molecule is immobilized to said waveguide film, with each of said waveguide channels having a different one of said species immobilized thereto.
- 7. The kit of claim 6, whose manufacture further includes a step of etching a plurality of spaced grooves into said surface of said substrate prior to said step of depositing said waveguide layer second optical material, wherein said grooves are oriented substantially perpendicular to said waveguide channels, and spacing of said grooves is selected to serve as a grating facilitating efficient coupling of light from an incident excitation beam into said parallel strips.
- 8. The kit of claim 1, wherein said second optical material is silicon oxynitride having an elemental ratio of approximately Si.sub.2 O.sub.3 N.
- 9. A method of making a waveguide biosensor for performing specific binding assays with an optical unit including a light source positioned to direct light into an optical structure for propagation by total internal reflectance therein and detection means oriented to detect light from a region proximal to the optical structure, said optical structure constructed by the steps of:
- providing a substrate comprising a first optical material of refractive index n.sub.1 and having two parallel planar surfaces separated by a thickness and a surrounding edge;
- depositing a waveguide layer of a second optical material having a refractive index n.sub.2 on one of said surfaces, said waveguide layer having a thickness which is between about 0.1 .mu.m and about 10 .mu.m and n.sub.2 being greater than n.sub.1 ;
- coating at least one region of said waveguide layer with a resist compound which resists an etchant to produce a coated region and an uncoated region;
- etching said waveguide layer with said etchant to remove said second optical material from the uncoated region;
- removing the resist compound from the etched waveguide later to expose the coated region; and
- immobilizing a plurality of capture molecules to said exposed coated region, said capture molecules being constructed to bind with specificity a selected analyte molecule.
- 10. The method of claim 9, further including a step of coating said exposed coated region with a coating which provides a level of nonspecific protein binding which is less than about 10% of a amount of said specific binding of said analyte, said coating being selected from the group consisting of: polymethacryloyl polymer, polyethyleneglycol, and avidin; and wherein in said step of immobilizing capture molecules, said capture molecules are bound to said coating.
- 11. The method of claim 10, wherein said second optical material is selected from the group consisting of: silicon oxynitride of refractive index n.sub.2, and deposited silicon dioxide; and said first optical material is selected from the group consisting of: deposited silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n.sub.1, and magnesium fluoride.
- 12. A kit for performing specific binding assays with an optical unit including a light source positioned to direct light into an optical substrate and detection means oriented to detect light from a region proximal to the waveguide, which includes:
- a biosensor comprising:
- a step gradient waveguide including:
- a substrate formed of a first optical material of refractive index n.sub.1 and having a surface disposed adjacent and in contact with a waveguide film formed of a second optical material having a refractive index n.sub.2 which is greater than n.sub.1 ; and
- a waveguide coupler contactingly disposed on said waveguide film, said waveguide coupler comprising:
- an input waveguide formed of an optical material having a refractive index n.sub.3, and having a thickness which is between about 0.5 mm and about 5 mm; and
- a spacing layer formed of an optical material having a refractive index n.sub.4, wherein n.sub.4 <n.sub.3 and n.sub.4 <n.sub.2, and having a thickness selected to optimize evanescent coupling of light from said input waveguide into said waveguide film and
- at least one specific binding molecule immobilized to said waveguide film and constructed to bind with specificity an analyte.
Government Interests
License Rights. The research supporting this invention was partially funded by National Institute of Health Grant #HL32132. The United States Government may have some rights in this invention.
US Referenced Citations (9)
Foreign Referenced Citations (2)
Number |
Date |
Country |
0 517 516 A1 |
Dec 1992 |
EPX |
0 519 623 A2 |
Dec 1992 |
EPX |