Claims
- 1. A method of making an aqueous dispersion of liposomes containing a biologically active compound and which consists essentially of mixing:
- a) at least one part, bilayer forming membrane lipid and
- b) at least one non-aqueous liquid consisting essentially of a water-miscible organic liquid which is a solvent for the lipid,
- the proportion by weight of a) to b) being from 40:1 to 1:20,
- with sufficient water such that liposomes are spontaneously formed, the biologically active compound being added prior to, together with or subsequent to the water and being finally present in an amount sufficient that a biologically effective dosage of the biologically active compound is associated with the liposomes.
- 2. The method as claimed in claim 1, wherein the water is added to the composition in at least two separate portions.
- 3. A method of making an aqueous dispersion of liposomes which consists essentially of mixing a pro-liposome composition comprising a uniform mixture of:
- a) at least part, bilayer forming one membrane lipid and
- b) at least one non-aqueous liquid consisting essentially of a water-miscible organic liquid which is a solvent for the lipid, and
- c) a biologically active compound,
- the proportion by weight of a) to b) being from 40:1 to 1:20,
- with sufficient water such that liposomes are spontaneously formed,
- component c) being present in an amount sufficient that a biologically effective dosage of the biologically active compound is associated with the liposomes and
- wherein the water is added to the composition in at least two separate portions.
- 4. A method of making an aqueous dispersion of liposomes containing a biologically active compound and which consists essentially of spraying an aerosol composition comprising in a volatile liquid propellant:
- a) at least one parts bilayer forming membrane lipid and
- b) at least one non-aqueous liquid consisting essentially of a water-miscible organic liquid which is a solvent for the lipid,
- the proportion by weight of a) to b) being from 40:1 to 1:20,
- so that it comes into contact with sufficient water such that liposomes are spontaneously formed,
- the biologically active compound being added prior to, together with or subsequent to the water and being finally present in an amount sufficient that a biologically effective dosage of the biologically active compound is associated with the liposomes.
- 5. The aqueous dispersion of liposomes produced by the method of claim 1, and comprising liposomes formed of membrane lipid which have diameters within the range of about 0.1 to 2.5 microns and contain at least 2 ml. of entrapped aqueous fluid per gram of the lipid, there being present in the aqueous dispersion of a water-miscible organic liquid which is a solvent for the lipid.
- 6. The aqueous dispersion of liposomes produced by the method of claim 4, and comprising liposomes formed of membrane lipid which have diameters within the range of about 0.1 to 2.5 microns and contain at least 2 ml. of entrapped aqueous fluid per gram of the lipid, there being present in the aqueous dispersion of a water-miscible organic liquid which is a solvent for the lipid.
Priority Claims (2)
Number |
Date |
Country |
Kind |
8406038 |
Mar 1984 |
GBX |
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8406919 |
Mar 1984 |
GBX |
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Parent Case Info
This is a continuation of Ser. No. 07/171,148, filed Mar. 21, 1988, now U.S. Pat. No. 5,004,611, issue date Apr. 2, 1991, which is a continuation of now-abandoned Ser. No. 06/709,796 filed Mar. 8, 1985.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4590060 |
Ehrenfeld |
May 1986 |
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Foreign Referenced Citations (5)
Number |
Date |
Country |
130577 |
Jan 1985 |
EPX |
0002404 |
Feb 1965 |
JPX |
60-89834 |
Jul 1981 |
JPX |
2145107 |
Mar 1985 |
GBX |
8303383 |
Oct 1983 |
WOX |
Non-Patent Literature Citations (3)
Entry |
Chemical abstracts 105:12154c (1986). |
Chemical abstracts 103:27787f (1985). |
Chemical abstracts 103:156792v (1985). |
Continuations (2)
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Number |
Date |
Country |
Parent |
171148 |
Mar 1988 |
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Parent |
709796 |
Mar 1985 |
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