Claims
- 1. A solution for the preservation of biological materials comprising raffinose, trimethyl amine oxide and sodium citrate, wherein the solution is isotonic with the material to be preserved and the solution is substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide.
- 2. The solution of claim 1, wherein the raffinose and trimethyl amine oxide are present in a molar ratio of between about 1.1 to 1 to about 2.0 to 1.
- 3. The solution of claim 1 wherein the raffinose and trimethyl amine oxide are present in a molar ratio of about 1.4 to 1 to about 1.8 to 1.
- 4. The solution of claim 1 additionally comprising calcium.
- 5. The solution of claim 4, wherein the calcium is present as a salt selected from the group consisting of calcium chloride and calcium sulfate and is present at a concentration of about 1.5 mM to about 2.0 mM.
- 6. The solution of claim 5, wherein the calcium chloride is present at a concentration of about 1.75 mM.
- 7. A concentrated form of a solution according to claim 4 that, upon addition of a specified amount of an aqueous solution, forms the solution according to claim 1.
- 8. A collection of solids that, upon addition of a specified amount of an aqueous solution, forms the solution according to claim 1.
- 9. A container comprising viable biological materials immersed in a solution according to claim 1.
- 10. A method for preserving the viability of a biological material comprising contacting the biological material with a solution comprising raffinose, trimethyl amine oxide and sodium citrate, the solution being substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide.
- 11. The method of claim 10 wherein the raffinose and trimethyl amine oxide are present in a molar ratio of between about 1.1 to 1 to about 2.0 to 1.
- 12. The method of claim 10, wherein the raffinose and trimethyl amine oxide are present in a molar ratio of about 1.6:1.
- 13. The method of claim 10, wherein the solution additionally comprises calcium.
- 14. The method of claim 13, wherein the calcium is present as a salt selected from the group consisting of calcium chloride and calcium sulfate and is present at a concentration of between about 1.5 mM and about 2.0 mM.
REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. application No. 08/722,306, filed Sep. 30, 1996, now U.S. Pat. 5,827,640, which is a continuation-in-part of U.S. application No. 08/662,244, filed Jun. 14, 1996 now U.S. Pat. No. 5,879,875.
US Referenced Citations (18)
Foreign Referenced Citations (1)
Number |
Date |
Country |
6786681 |
Nov 1984 |
AUX |
Continuation in Parts (2)
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Number |
Date |
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Parent |
722306 |
Sep 1996 |
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Parent |
662244 |
Jun 1996 |
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