Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease

Abstract
The present invention relates to a composition comprising separated or proliferated cells from umbilical cord blood for treating developmental and/or chronic lung disease, more precisely a composition containing separated or proliferated cells from umbilical cord blood for intratracheal administration for treating developmental and/or chronic lung disease. The cells of the composition of the present invention have excellent activities of proliferation and differentiation, so that the extraction, separation and selection of a tissue donor are all very easy. Besides, the composition of the invention can be very effectively used for the treatment of developmental and/or chronic diseases by intratracheal administration.
Description

DESCRIPTION OF DRAWINGS

The application of the preferred embodiments of the present invention is best understood with reference to the accompanying drawings, wherein:



FIG. 1-FIG. 3 are photographs illustrating that the pathological findings about lung tissues of neonatal rats induced with bronchopulmonary dysplasia, one of the developmental and/or chronic lung diseases, after treating with the method of the invention. FIG. 1 represents the normal control (NC), FIG. 2 represents the hyperoxia-exposed group (HC), and FIG. 3 represents the hyperoxia-exposed and umbilical cord blood mesenchymal stem cell transplanting group (HT).



FIG. 4 is a graph illustrating the alveolar development in the lung of a neonatal rat induced with bronchopulmonary dysplasia, one of the developmental and/or chronic lung disease, after treating with the method of the invention,


NC: normal control


HC: hyperoxia-exposed group (bronchopulmonary dysplasia)


HT: hyperoxia-exposed and umbilical cord blood mesenchymal stem cell transplanting group


*significant difference with NC group (p<0.05)


#significant difference with HC group (p<0.05)



FIG. 5 is a photograph illustrating that the therapeutic cell component was safely located by the method of the invention in the lung of a neonatal rat induced with bronchopulmonary dysplasia,


Blue: nuclei stained with DAPI,


Red: labeled with PKH26


Claims
  • 1. A composition for treating developmental and/or chronic lung disease comprising cells separated or proliferated from umbilical cord blood.
  • 2. The composition for treating developmental and/or chronic lung disease according to claim 1, wherein the cells are one or more cells selected from a group consisting of monocytes, such as autologous hematopoietic stem cells, and mesenchymal stem cells separated from umbilical cord blood and mesenchymal stem cells sub-cultured and amplified from umbilical cord blood derived mesenchymal stem cells.
  • 3. The composition for treating developmental and/or chronic lung disease according to claim 1, which additionally includes a medium for those separated and proliferated cells.
  • 4. The composition for treating developmental and/or chronic lung disease according to claim 3, wherein the medium is selected from a group consisting of DMEM, MEM, alpha-MEM, McCOys 5A medium (Gibco), Eagle's basal medium, CMRL medium, Glasgow minimum essential medium, Ham's F-12 medium, Iscove's modified Dulbecco's medium, Liebovitz' L-15 medium and RPMI 1640 medium.
  • 5. The composition for treating developmental and/or chronic lung disease according to claim 1, wherein the composition is intratracheally administered into a patient.
  • 6. The composition for treating developmental and/or chronic lung disease according to claim 1, wherein the chronic lung disease is selected from a group consisting of cystic fibrosis, chronic obstructive pulmonary disease or pulmonary dysplasia.
  • 7. The composition for treating development and/or chronic lung disease according to claim 1, wherein the composition includes one or more subsidiary components selected from a group consisting of serum, antibiotics and antifungal agents.
  • 8. The composition for treating developmental and/or chronic lung disease according to claim 1, wherein the composition includes one or more subsidiary components selected from a group consisting of fetal bovine serum, horse serum, human serum, Penicillin G, streptomycin sulfate, amphotericin B, gentamycin and nystatin.
Priority Claims (1)
Number Date Country Kind
10-2006-0009625 Feb 2006 KR national