Composition of matter and process

BACKGROUND OF THE INVENTION
Antibiotic U-60,394 has similarities to matchamycin, a known antibiotic. See A. Kimura and H. Nishimura, J. Antibiotics 33, 461 (1970). Matchamycin has the molecular formula C.sub.20 H.sub.13 O.sub.6 N.sub.3 Cu and is produced by S. amagasakensis. It can be differentiated from U-60,394 by melting point, UV spectrum, IR spectrum, and mass spectrum. S. amagasakensis grown in U-60,394 medium does not produce U-60,394, nor does S. woolensis when grown in the Japanese medium produce matchamycin.
BRIEF SUMMARY OF THE INVENTION
Antibiotic U-60,394 is producible in a fermentation under controlled conditions using a biologically pure culture of the new microorganism Streptomyces woolensis, Dietz and Li sp.n., NRRL 12113.
Antibiotic U-60,394 is strongly active against various Gram-positive bacteria. Further, the base addition salts of antibiotic U-60,394 are also active against these bacteria. Thus, antibiotic U-60,394 and its salts can be used to disinfect washed and stacked food utensils contaminated with S. aureus. They can also be used as disinfectants on various dental and medical equipment contaminated with S. aureus. Still further, antibiotic U-60,394 and its salts can be used as a bacteriostatic rinse for laundered clothes, and for impregnating papers and fabrics; and, they are also useful for suppressing the growth of sensitive organisms in plate assays and other microbiological media.

DETAILED DESCRIPTION OF THE INVENTION
Chemical and Physical Properties of Antibiotic U-60,394:
Molecular Weight: 391.08014 (high resolution spectrometry)
Molecular Formula: C.sub.20 H.sub.13 N.sub.3 O.sub.6
Color and Form of Crystals: Yellowish-green needles.
Ultraviolet Absorption Spectrum:
The UV spectrum of antibiotic U-60,394 is shown in FIG. 3 of the drawings. The solution of antibiotic U-60,394 in methanol displayed absorption as follows:
______________________________________Solvent .lambda. max Absorptivity (.epsilon.)______________________________________Methanol 310 nm 22,2750.01N H.sub.2 SO.sub.4 in MeOH 311 22,8570.01N KOH in MeOH 303; 347 15,796; 10,389______________________________________
Melting Point: 265.degree. C. to 266.degree. C. with decomposition.
Infrared Absorption Spectrum:
Antibiotic U-60,394 has a characteristic infrared absorption spectrum in a mineral oil mull as shown in FIG. 4 of the drawings. Peaks are observed at the following wave lengths.
______________________________________Band BandFrequency.sup.1 Intensity.sup.2 Frequency.sup.1 Intensity.sup.2______________________________________3342 30 1298 223289 22 1256 473098 45 1237 523086 44 1229 502955 2 1220 482924 1 1186 492869 7,sh 1172 532854 2 1136 612728 62 1121 672630 65 1100 561978 83 1052 611918 85 1038 711868 87 1022 76,sh1848 88 996 781764 19 964 461714 76,sh 932 841652 21 914 761625 62 893 481598 47 867 771587 46 849 761579 51 810 381540 11 802 701458 7 789 571451 15 763 531424 43 757 221377 35 740 421359 23 722 181339 32 712 321328 29 692 591315 24 678 60 656 33 625 36______________________________________ .sup.1 Wavenumbers (cm.sup.-1) .sup.2 Percent transmittance (% T), sh. = shoulder Intensity at 3800 cm.sup.1 is 59% T. Minimum intensity at 1804 cm.sup.-1 is 89.6% T.
.sup.13 C-Nuclear Magnetic Resonance (NMR) Spectrum:
The .sup.13 C-NMR spectrum of antibiotic U-60,394 is shown in FIG. 1 of the drawings. The .sup.13 C-NMR spectrum was observed on a Varian CFT-20 Spectrometer on a solution (ca. 0.5 ml., ca. 200 mg./ml.) of the sample of the antibiotic in deutero-dimethylsulfoxide (d.sub.6 -DMSO). The spectrum was calibrated against internal tetramethylsilane and frequencies were recorded in ppm downfield from tetramethylsilane.
Proton Magnetic Resonance (.sup.1 H-NMR) Spectrum:
The .sup.1 -H-NMR spectrum of antibiotic U-60,394 at 100 MHZ is shown in FIG. 2 of the drawings. The .sup.1 H-NMR spectrum was observed on a Varian XL-100-15 Spectrometer on a solution (ca. 0.5 ml., ca. 150 mg./ml.) of the sample of the antibiotic in deutero-dimethylsulfoxide (d.sub.6 -DMSO). The spectrum was calibrated against internal tetramethylsilane and frequencies were recorded in ppm downfield from tetramethylsilane.
Solubilities:
Antibiotic U-60,394 is soluble in methanol and acetone with difficulty, and easily soluble in ethyl acetate, methylene chloride, and dimethylsulfoxide. It is insoluble in water.
Antimicrobial Spectrum of Antibiotic U-60,394:
Antibiotic U-60,394 is active against various Gram-positive bacteria and S. pneumoniae as shown in the following tables.
Assay:
The antibacterial assay is a standard microplate broth dilution assay. The MIC is determined by standard methods using two-fold dilutions of the antibiotic in Brain Heart Infusion Broth (Difco Lab., Detroit, Mi). The inocula are overnight cultures of the test organisms, diluted so that the final population contains approximately 10.sup.5 cells/ml. The solutions are incubated at 28.degree. to 37.degree. C. for 24 hours. The lowest antibiotic concentration which allows no growth=MIC or minimum inhibitory concentration.
______________________________________ Minimum InhibitoryMicroorganism Concentration (mcg/ml)______________________________________Staphylococcus aureus 284 UC 76 7.8Staphylococcus aureus UC 570 2.0Staphylococcus aureus UC 746 7.8Streptococcus hemolyticus UC 152 .ltoreq.0.5Streptococcus faecalis UC 694 500Escherichia coli UC 45 >1000Proteus vulgaris UC 93 >1000Klebsiella pneumoniae UC 58 >1000Salmonella schottmuelleri UC 126 >1000Pseudomonas aeruginosa UC 95 >1000Streptococcus pneumoniae UC 41 1.0______________________________________
"UC" is a registered trademark of The Upjohn Company Culture Collection. These cultures can be obtained from The Upjohn Company in Kalamazoo, Mi., upon request.
THE MICROORGANISM
The microorganism used for the production of antibiotic U-60,394 is a biologically pure culture of Streptomyces woolensis, Dietz and Li sp.n., NRRL 12113.
A subculture of this microorganism can be obtained from the permanent collection of the Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Il., U.S.A. Its accession number in this depository is NRRL 12113. It should be understood that the availability of the culture does not constitute a license to practice the subject invention in derogation of patent rights granted with the subject instrument by governmental action.
The microorganism of this invention was studied and characterized by Alma Dietz and Grace P. Li of The Upjohn Research Laboratories.
S. woolensis was found to produce an antibiotic very similar to matchamycin. S. woolensis was found to be very similar on Ektachrome (Table 1) to the matchamycinproducer Streptomyces amagasakensis E-753 (Kimura, A., and H. Nishimura. 1970. Matchamycin: A new antibiotic produced by Streptomyces E-753, J. Antibiotics 23:461-463). Both cultures are melanin negative. They are both members of the genus Streptomyces as determined by the detection of L-diaminopimelic acid in whole cell hydrolysates, the presence of vegetative hyphae producing a well-developed mycelium, and the production of aerial mycelium which sporulates to form a mass of color. The sporulating mycelial mass of the two cultures is superficially similar. The spores of the new culture are in long flexuous to open spiral chains; the spores of S. amagasakensis are in open to tight spiral chains. This may be observed by light and scanning electron microscopy. The surface detail of the spores of the two cultures, as observed by scanning electron microscopy, is distinctly different. The new culture has spores with a type of surface appendage not reported in the literature for members of the genus Streptomyces. The spore surface is covered with wooly-like protrusions which overlap to give a matted appearance. The spore surface of S. amagasakensis is smooth with a slight ridging to spiny. The spines are abundant and short. The spores of both cultures are spherical to elliptical. The cultures are further differentiated by their reference color characteristics (Table 2) and their growth on carbon compounds in the synthetic medium of Shirling and Gottlieb (Table 3). There are no significant differences in their cultural and biochemical characteristics (Table 4).
The cultures in their color pattern on Ektachrome could be considered related. However, the distinctly different microscopic characteristics and the subtle differences in growth on carbon compounds and in reference color characteristics warrant the designation of a new species of Streptomyces. The name Streptomyces woolensis is proposed based on the distinctive wooly appearance of the spores of the new culture. It is proposed that this type species be designated the type subspecies should cultures with similar properties be found. This is in accordance with the Rules of Nomenclature in the International Code of Nomenclature of Bacteria (Lapage, S. P., P. H. A. Sneath, E. F. Lessel, V. B. D. Skerman, H. P. R. Seeliger, and W. A. Clark, eds. 1975. International Code of Nomenclature of Bacteria, 1976 Revision. American Society for Microbiology, Washington, D.C.).
Streptomyces woolensis Dietz and Li sp.nov. NRRL 12113.
Color Characteristics:
The aerial mycelium is reddish to brownish gray on Ektachrome (Table 1) and gray (Gy) when compared with the chips in the Tresner and Backus (Tresner, H. D., and E. J. Backus. 1963. System of color wheels for streptomycete taxonomy. Appl. Microbiol. 11:335-338) color wheels. Reference color characteristics are given in Table 2. The culture is melanin negative.
Microscopic Characteristics:
Spores are borne in long, flexuous to open spiral chains. The spores, when observed by the scanning electron microscope, are found to be covered with wooly appendages. The wooly appendages look like intertwined fibers. (This surface detail has not been reported in the literature.)
Growth on Carbon Compounds:
See Table 3.
Whole Cell Analysis:
L-diaminopimelic acid was detected.
Cultural and Biochemical Characteristics:
See Table 4.
Temperature:
Growth was good at 24.degree. C.-32.degree. C., moderate at 18.degree. C. and 37.degree. C. There was no to trace growth at 45.degree. C. and no growth at 55.degree. C.
The methods used herein are those cited in Becker et al. (Becker, B., M. P. Lechevalier, and H. A. Lechevalier. 1966. Chemical composition of cell wall preparations from strains of various form-genera of aerobic actinomycetes. Appl. Microbiol. 13:236-243), Dietz (Dietz, A. 1954. Ektachrome transparencies as aids in actinomycete classification. Ann. N. Y. Acad. Sci. 60: 152-154) (Dietz, A. 1967. Streptomyces steffisburgensis sp. n. J. Bacteriol. 94:2022-2026), Dietz and Mathews (Dietz, A., and J. Mathews. 1971. Classification of Streptomyces spore surfaces into five groups. Appl. Microbiol. 21:527-533), and Shirling and Gottlieb (Shirling, E. B., and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16:313-340).
TABLE 1__________________________________________________________________________Color Characteristics* of Streptomyces woolensis and Streptomycesamagasakensis on Ektachrome** S. woolensis S. amagasakensisAgar Determi- NRRL 12113 ATCC 21325Medium nation Chip Color Chip Color__________________________________________________________________________Bennett's S 63 light brownish gray 23 dark reddish gray R 77 moderate yellowish 77 moderate yellowish brown brownCzapek's S 63 light brownish gray 266 dark graysucrose R 77 moderate yelowish 63 light brownish brown grayMaltose- S 63 light brownish gray 23 dark reddish graytryptone R 77 moderate yellowish 55 strong brown brownPeptone- S 70 light orange yellow 70 light orange yellowiron R 68 strong orange 68 strong orange yellow yellow0.1% S 22 reddish gray 22 reddish graytyrosine R 38 dark reddish orange 38 dark reddish orangeCasein S 23 dark reddish gray 23 dark reddish graystarch R 63 light brownish gray 63 light brownish gray__________________________________________________________________________ *Color was determined by comparison with NBS Color Chips (SP 440. Color: Universal Language and Dictionary of Names. U.S. Government Printing Office, Washington, D.C. 20402) (SRM 2106. ISCCNBS Centroid Color Charts. Office of Standard Reference Material, Room B311, Chem. Building. Nationa Bureau of Standards, Washington, D.C. 20234). S = Surface R = Reverse Color determinations made on tubes incubated 7 days at 28.degree. C. **(Dietz, A. 1954. Ektachrome transparencies as aids in actinomycete classification. Ann. N.Y. Acad. Sci. 60:152-154.
TABLE 2__________________________________________________________________________Reference Color Characteristics* of S. woolensis and S. amagasakensis S. woolensis S. amagasakensis Determi- NRRL 12113 ATCC 21325Agar Medium nation Chip Color Chip Color__________________________________________________________________________Bennett's S 89 pale yellow 22 reddish gray R 89 pale yellow 72 dark orange yellow P -- -- -- --Czapek's S 10 pinkish gray 23 dark reddish graysucrose R 86 light yellow 90 grayish yellow P 89 pale yellow -- --Maltose S 22 reddish gray 63 light brownish graytryptone R 78 dark yellowish brown 75 deep yellowish brown P 89 pale yellow 86 light yellowYeast extract- S 23 dark reddish gray 23 dark reddish graymalt extract R 58 moderate brown 71 moderate olive(ISP-2) P 71 moderate olive 71 moderate oliveOatmeal S 22 reddish gray 23 dark reddish gray(ISP-3) R 86 light yellow 91 dark grayish yellow P 89 pale yellow 87 moderate yellowInorganic S 23 dark reddish gray 23 dark reddish graysalts starch R 87 moderate yellow 75 deep yellowish brown(ISP-4) P -- -- 87 moderate yellowGlycerol- S 93 yellowish gray 63 light brownish grayasparagine R 71 moderate orange yellow 71 moderate orange yellow(ISP-5) P 89 pale yellow 87 moderate yellow__________________________________________________________________________ *Determined by comparison with NBS Color Chips (SP 440. Color: Universal Language and Dictionary of Names. U.S. Government Printing Office, Washington, D.C. 20402) (SRM 2106. ISCCNBS Centroid Color Charts. Office of Standard Reference Material, Room B311, Chem. Building, National Burea of Standards, Washington, D.C. 20234). S + Surface R = Reverse P = Pigment Color determinations made on plates incubated 14 days at 28.degree. C.
TABLE 3______________________________________Growth of S. woolensis and S. amagasakensis on Carbon Compoundsin the Synthetic Medium of Shirling and Gottlieb*Synthetic Medium S. woolensis S. amagasakensis(ISP-9) NRRL 12113 ATCC 21325______________________________________Negative Control - +(No carbon compound)Positive Control + +D-glucose)L-arabinose + ++Sucrose ++ ++D-xylose ++ ++Inositol ++ ++D-mannitol ++ ++D-fructose ++ ++Rhamnose ++ ++Raffinose - .+-.Cellulose - .+-.______________________________________ ++ = Strong utilization + = Positive utilization .+-. = Doubtful utilization - = Negative utilization *Shirling, E. B., and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16:313-340.
TABLE 4__________________________________________________________________________Cultural and Biochemical Characteristics* of S. woolensisand S. amagasakensis Determi- S. woolensis S. amagasakensisMedium nation NRRL 12113 ATCC 21325__________________________________________________________________________AgarPeptone-iron S Trace lavender-gray Fair pale cream-white R Pale yellow Pale yellow-tan P -- -- O Melanin negative Melanin negativeCalcium S Pale gray-tan Gray-tanmalate R Cream-white Pale cream-yellow P -- -- O Malate partially Malate partially solubilized solubilizedGlucose S Lavender-gray with Lavender-gray center;asparagine cream-white sectors cream-white edge R Yellow-tan Yellow-tan P Pale yellow Pale yellowSkim milk S Cream-white Cream-white R Yellow-tan Orange-tan P Yellow Orange O Casein solubilizedTyrosine S Lavender-gray-cream Cream center; lavender- gray edge R Orange-tan Tan P Orange-tan Tan O Tyrosine solubilized Tyrosine solubilizedXanthine S Lavender-gray Lavender-gray center; white edge R Very pale yellow Very pale yellow P Very pale yellow Very pale yellow O Xanthine not solubil- Xanthine not solubil- ized izedNutrient S Lavender-gray Mottled lavender-grayStarch cream R Pale yellow Yellow P -- -- O Starch slightly Starch solubilized solubilizedYeast extract S Lavender-gray cream Lavender-gray creammalt extract R Yellow-tan Yellow-tan P Pale yellow YellowPeptone- S Pale yellow vegeta- Trace, very pale gray-yeast tive whiteextract iron R Pale yellow Yellow(ISP-6) P Pale yellow Yellow O Melanin negative Melanin negativeTyrosine S Lavender-gray Pale lavender-gray(ISP-7) R Pale yellow Pale yellow P Pale yellow Very pale yellow O Melanin negative Melanin negativeGelatinPlain S Colorless or light Tan surface pellicle tan surface ring P -- -- O Liquefaction - 1/4 Liquefaction - 1/2 Growth at bottom of (1 tube); tube - colorless complete (1 tube)Nutrient S Tan surface ring Tan surface pellicle P -- -- O Trace liquefaction Liquefaction - 1/2 (1 tube); complete (1 tube)BrothSynthetic S -- White aerial on surfacenitrate pellicle P -- Pale yellow O Flocculent, color- Flocculent, colorless less bottom growth bottom growth Nitrate reduced to Nitrate reduced to nitrite nitriteNutrient S Yellow ring Lavender-gray pinknitrate aerial growth on surface pellicle P -- -- O Flocculent yellow Flocculent yellow bottom growth bottom growth Nitrate reduced to Nitrate reduced to nitrite nitriteLitmus milk S Blue-gray-tan ring Blue-gray-tan ring Gray-pink aerial Trace gray aerial growth on surface growth on surface pellicle pellicle P Red to lavender Red-tan O Peptonization Peptonization Litmus reduced in Litmus reduced in one one of two tubes of two tubes pH 7.5 pH 7.2__________________________________________________________________________
The compound of the invention process is produced when the elaborating organism is grown in an aqueous nutrient medium under submerged aerobic conditions. It is to be understood, also, that for the preparation of limited amounts surface cultures and bottles can be employed. The organism is grown in a nutrient medium containing a carbon source, for example, an assimilable carbohydrate, and a nitrogen source, for example, an assimilable nitrogen compound or proteinaceous material. Preferred carbon sources include glucose, brown sugar, sucrose, glycerol, starch, cornstarch lactose, dextrin, molasses, and the like. Preferred nitrogen sources include cornsteep liquor, yeast, autolyzed brewer's yeast with milk solids, soybean meal, cottonseed meal, cornmeal, milk solids, pancreatic digest of casein, fish meal, distillers' solids, animal peptone liquors, meat and bone scraps, and the like. Combinations of these carbon and nitrogen sources can be used advantageously. Trace metals, for example, zinc, magnesium, manganese, cobalt, iron, and the like, need not be added to the fermentation media since tap water and unpurified ingredients are used as components of the medium prior to sterilization of the medium.
Production of the compound by the invention process can be effected at any temperature conducive to satisfactory growth of the microorganism, for example, between about 18.degree. and 40.degree. C., and preferably between about 20.degree. and 28.degree. C. Ordinarily, optimum production of the compound is obtained in about 3 to 15 days. The medium normally remains alkaline during the fermentation. The final pH is dependent, in part, on the buffers present, if any, and in part on the initial pH of the culture medium.
When growth is carried out in large vessels and tanks, it is preferable to use the vegetative form, rather than the spore form, of the microorganism for inoculation to avoid a pronounced lag in the production of the compound and the attendant inefficient utilization of the equipment. Accordingly, it is desirable to produce a vegetative inoculum in a nutrient broth culture by inoculating this broth culture with an aliquot from a soil, liquid N.sub.2 agar plug, or a slant culture. When a young, active vegetative inoculum has thus been secured, it is transferred aseptically to large vessels or tanks. The medium in which the vegetative inoculum is produced can be the same as, or different from, that utilized for the production of the compound, so long as a good growth of the microorganism is obtained.
A variety of procedures can be employed in the isolation and purification of the compound produced by the subject invention from fermentation beers. Isolation can be accomplished by extraction with solvents such as methylene chloride, acetone, butanol, ethyl acetate and the like; and silica gel chromatography can be used to purify crude preparations of the antibiotic.
In a preferred recovery process, the compound produced by the subject process is recovered from the culture medium by separation of the mycelia and undissolved solids by conventional means, such as by filtration or centrifugation and solvent extraction of the filtered broth. The filtrate can be extracted with a solvent for antibiotic U-60,394, for example, methylene chloride, and the extract evaporated under reduced pressure to an aqueous concentrate. This preparation can be purified by chromatography on silica gel. The solvent system used for the chromatography is ethyl acetate:acetone:water (8:5:1) (v/v). Antibiotic U-60,394 is shown at R.sub.f 0.4 using standard bioautography on Streptococcus pyogenes UC 6055.
The antibiotic of the subject invention also can be recovered from fermentation broth by resin sorption on a resin comprising a non-ionic macro porous copolymer of styrene cross linked with divinylbenzene. Suitable resins are Amberlite XAD-2 and XAD-7, according to the procedure disclosed in U.S. Pat. No. 3,515,717. (Amberlite resins are available from Rohm and Haas, Philadelphia, Pa.). The antibiotic can be eluted from said resins by using acetone.
Salts of antibiotic U-60,394 also can be formed with inorganic or organic bases. Such salts can be prepared, as for example, by suspending antibiotic U-60,394 in water, adding a dilute base until the pH of the suspension is about 10.0 to 11.0, and freeze-drying to provide a dried residue consisting of the U-60,394 salt. Antibiotic U-60,394 salts with inorganic cations which can be formed include the sodium, potassium, and calcium salts. Other salts of U-60,394, including those with inorganic bases such as primary, secondary, and tertiary monoamines as well as with polyamines, also can be formed using the above-described or other commonly employed procedures. Other valuable salts are obtained with therapeutically effective bases which impart additional therapeutic effects thereto. Such bases are, for example the purine bases such as theophyllin, thiobromin, caffeine, or derivatives of such purine bases; antihistaminic bases which are capable of forming salts with weak acids, pyridine compounds such as nicotinic acid amide, isonicotinic acid hydrazide, and the like; phenylalkylamines such as adrenaline, ephedrine, and the like; choline, and others. Salts of U-60,394 can be used for the same biological purposes as the parent compound.
The following examples are illustrative of the process and product of the invention, but are not to be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted.
EXAMPLE 1
A. Fermentation
A biologically pure culture of Streptomyces woolensis Dietz and Li sp.n., NRRL 12113, is used to inoculate 500 -ml. Erlenmeyer seed flasks containing 100 ml. of sterile medium consisting of the following ingredients:
______________________________________ g./liter______________________________________Glucose monohydrate 10Difco peptone 10Difco yeast extract 2.5Deionized water q.s. 1 liter______________________________________
Difco products can be obtained from Difco Laboratories, Detroit, Mi.
The seed medium post-sterilization pH is .about. 6.5. The seed inoculum is grown for three days at 28.degree. C. on a Gump rotary shaker operating at 250 rpm and having a 21/2inch stroke.
Seed inoculum (100 ml.), prepared as described above, is used to inoculate 500-ml. fermentation flasks (Erlenmeyer) containing 100 ml. of sterile fermentation medium consisting of the following ingredients:
______________________________________ g./liter______________________________________Cerelose 10Buffalo starch* 10Phytone (BBL)** 10CaCO.sub.3 5NaCl 2Tap water Balance______________________________________ *now called National Starch. Fisher Scientific **BBL, Div. of Becton, Dickinson & Co., Cockeysville, Md. 21030 U.S.A.
The inoculated fermentation medium is incubated at a temperature of 28.degree. C. for 6 days on a Gump rotary shaker operating at 250 rpm and having a 21/2inch stroke.
A typical six-day fermentation has the following titers of antibiotic in the fermentation broth:
______________________________________Day Assay, BU/ml______________________________________2 2.33 3.24 4.05 4.56 4.4______________________________________
The assay is a Sarcina lutea disc plate assay using 0.1 M Tris buffer, pH 7.0 as diluent.
B. Recovery
To whole beer (ca. 10 1.) from a fermentation, as described above, is added 1 liter of diatomaceous earth (dicalite) at harvest pH 8.2. This slurry is filtered over a bed of Dicalite 4200 with suction. The filtrate is adjusted to about pH 4 with sulfuric acid and extracted 3 times with 1/3 volume methylene chloride. The combined organic phases are concentrated to 50 ml. on a rotary evaporator. Thin layer chromatography of this solution using silica gel with 8:5:1 (v/v) ethyl acetate:acetone:water shows antibiotic U-60,394 at R.sub.f 0.4 using bioautography on S. pyogenes UC 6055.
About 540 mg. of a prep of U-60,394, obtained as described above, is dissolved in 5 ml. of 5:3 (v/v) chloroform:methanol. The solution is loaded onto a 2.5.times.100 cm silica gel column. The column is eluted with three bed volumes of 8:5 (v/v) ethyl acetate:acetone and the activity is eluted by switching to 8:5:0.5 (v/v) ethyl acetate:acetone:H.sub.2 O. The active fractions are located by dipping 12.7 mm pads, removing the solvent and incubating on S. pyogenes-seeded agar trays. U-60,394 has an R.sub.f of +0.4 in 8:5:1 v/v ethyl acetate:acetone:water and reacted with ferric chloride spray (1:1 methanol: 5% FeCT.sub.3) to give a yellow-orange color. The eluates are pooled and then concentrated on a rotary evaporator to give a solid preparation of essentially pure U-60,394. This material is dissolved in hot methanol and filtered. Water is added to cloudiness, and the solution is allowed to cool to room temperature. Yellowish-green crystalline needles of antibiotic U-60,394 form and are collected by standard procedures.

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