Claims
- 1. An isolated polynucleotide comprising:
a) a nucleotide sequence encoding a polypeptide that alters tocol content, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8 or 10 have at least 70% sequence identity, or b) a nucleotide sequence that is complementary to the nucleotide sequence of a), wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.
- 2. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 75% identity.
- 3. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 80% identity.
- 4. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 85% identity.
- 5. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 90% identity.
- 6. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 95% identity.
- 7. An expression cassette comprising the polynucleotide of claim 1, wherein the polynucleotide is operably linked to at least one regulatory sequence.
- 8. The expression cassette of claim 7, wherein the regulatory sequence is a promoter.
- 9. A non-human host cell transformed with the expression cassette of claim 7.
- 10. The host cell of claim 9, wherein the host cell is a plant cell.
- 11. A transformed plant comprising in its genome at least one stably incorporated polynucleotide of claim 1 operably linked to a promoter that drives expression in a plant cell.
- 12. The plant of claim 11, wherein the promoter is operably linked to the nucleotide sequence for the production of antisense transcripts.
- 13. The plant of claim 11, wherein the promoter is selected from the group consisting of seed-preferred, constitutive, chemically regulated, tissue-preferred, and developmentally regulated promoters.
- 14. The plant of claim 11, wherein the plant is a monocot.
- 15. The plant of claim 14, wherein the monocot is selected from the group consisting of maize, wheat, rice, sorghum, barley, millet and rye.
- 16. The plant of claim 11, wherein the plant is a dicot.
- 17. The plant of claim 16, wherein the dicot is selected from the group consisting of soybean, Brassica sp., alfalfa, safflower, sunflower, cotton, peanut and potato.
- 18. Transformed seed of the plant of claim 11.
- 19. The seed of claim 18 wherein the tocol content is increased.
- 20. The seed of claim 19 wherein the tocol content is increased to between 70 and 575 ppm.
- 21. The seed of claim 20 wherein the tocol content is increased to between 150 and 350 ppm.
- 22. The seed of claim 21 wherein the tocol content is increased to between 200 and 300 ppm.
- 23. The seed of claim 19 wherein the tocols are tocotrienols.
- 24. The seed of claim 23 wherein the tocotrienols are increased to between 19 and 461 ppm.
- 25. The seed of claim 24 wherein the tocotrienols are increased to between 100 and 400 ppm.
- 26. The seed of claim 25 wherein the tocotrienols are increased to between 250 and 350 ppm.
- 27. An isolated nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7 or 9; b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8 or 10; c) a nucleotide sequence having at least 80% sequence identity to the entire coding sequence of at least one of the nucleotide sequences set forth in a), wherein the nucleotide sequence encodes a polypeptide that alters tocol content; and d) a nucleotide sequence that is complementary to the nucleotide sequence of a), b), c) or d), wherein the nucleotide sequence is capable of the production of antisense transcripts in a cell.
- 28. An expression cassette comprising the nucleotide molecule of claim 27, wherein the nucleotide sequence is operably linked to at least one regulatory sequence.
- 29. The expression cassette of claim 28, wherein the regulatory sequence is a promoter.
- 30. A non-human host cell transformed with the expression cassette of claim 28.
- 31. The host cell of claim 30, wherein the host cell is a plant cell.
- 32. A transformed plant comprising in its genome at least one stably incorporated nucleotide sequence of claim 27 operably linked to a promoter that drives expression in a plant cell.
- 33. The plant of claim 32, wherein the promoter is operably linked to the nucleotide sequence for the production of antisense transcripts.
- 34. The plant of claim 32, wherein the promoter is selected from the group consisting of seed-preferred, constitutive, chemically regulated, tissue-preferred, and developmentally regulated promoters.
- 35. The plant of claim 32, wherein the plant is a monocot.
- 36. The plant of claim 35, wherein the monocot is selected from the group consisting of maize, wheat, rice, sorghum, barley, millet and rye.
- 37. The plant of claim 32, wherein the plant is a dicot.
- 38. The plant of claim 37, wherein the dicot is selected from the group consisting of soybean, Brassica sp., alfalfa, safflower, sunflower, cotton, peanut and potato.
- 39. Transformed seed of the plant of claim 32.
- 40. A method for altering tocotrienol as compared to wild-type, in a plant or part thereof, the method comprising transforming the plant with a nucleotide construct comprising a nucleotide sequence of claim 1.
- 41. The method of claim 40 wherein the tocotrienol is increased.
- 42. The method of claim 43, wherein the part is seed or grain.
- 43. The method of claim 43, wherein the nucleotide construct further comprises an operably linked promoter that drives expression in a plant cell.
- 44. The method of claim 43, wherein the promoter is selected from the group consisting of seed-preferred, constitutive, chemically regulated, tissue-preferred, and developmentally regulated promoters.
- 45. The method of claim 43, wherein the promoter is operably linked to the nucleotide sequence for the production of antisense transcripts.
- 46. The method of claim 41 wherein antioxidant activity in the plant or plant part is increased.
- 47. A method for altering tocols as compared to wild-type, in a non-human host cell, the method comprising transforming the host cell with a nucleotide construct comprising a nucleotide sequence of claim 1.
- 48. The method of claim 47 wherein the tocol content is increased.
- 49. The method of claim 48, wherein the host cell is a microbe.
- 50. The method of claim 49 wherein the microbe is a photosynthetic microbe.
- 51. The method of claim 48, wherein the nucleotide construct further comprises an operably linked promoter that drives expression in the host cell.
- 52. The method of claim 48, wherein the host cell comprises a cell from green plant tissue, root tissue, tuber tissue, flower tissue or grain tissue.
- 53. The method of claim 48, where the host cell has increased antioxidant activity.
- 54. An isolated polypeptide that alters tocol content , wherein the polypeptide amino acid sequence comprises at least four conserved motifs selected from the group consisting of SEQ ID NOS: 56-66.
- 55. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of claim 53.
- 56. An isolated polynucleotide that is complementary to the polynucleotide of claim 55.
- 57. An isolated polypeptide that alters tocol content, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8 or 10 have at least 70% identity.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. application Ser. No. 60/366,757 filed Mar. 22, 2002, which is herein incorporated in its entirety by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60366757 |
Mar 2002 |
US |