Patent Application
20230158139
The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety for all purposes. The ASCII copy, created on Oct. 30, 2020, is named 103550-732876_16-052-USCIP_ST25.txt and is 4.00 Kilobytes in size.
Embodiments of the instant disclosure relate to novel immunostimulatory compositions and their use to stimulate non-specific or modified immune responses to treat or reduce the risk of onset of a condition and/or infection. In certain embodiments, immunostimulatory compositions disclosed herein can be introduced to a subject to enhance innate immunity in the subject in order to treat or reduce the risk of onset of a health condition.
There is a growing need for new approaches for generating non-specific protection from viral and bacterial infections without having to resort to the use of antibiotics or other antimicrobial drugs, which serve to stimulate the development of antibiotic resistance and have other adverse effects. Currently however there are few immunostimulatory compounds that are capable of eliciting rapid and sustained activation of innate immune responses at mucosal surfaces such as the nasopharynx, upper respiratory tract, eye, GI tract, and reproductive tract to generate protection from infection. There is a need in the art for novel compositions and methods to enhance innate immune responses; for example, at mucosal surfaces for non-specific protection from infections, as well as to increase the efficacy of existing vaccines.
In certain embodiments disclosed herein, immunostimulatory compositions including at least one of (a) cationic liposomes, at least one (b) toll like receptor (TLR) or mixture thereof, and at least one (c) cellular adhesion agent. In certain embodiments, the TLRs include but are not limited to TLR3 and TLR9 ligands. In other embodiments, the cationic liposomes can include, but are not limited to, a mixture of cationic lipid and non-charged lipids. In accordance with these embodiments, a mixture of cationic lipid and non-charged lipids can include a mixture of DOTAP and cholesterol. In certain embodiments, the DOTAP and cholesterol can be about a 1:1, 2:1, 1:2, 3:1, 1:3 or other suitable ratio. In certain embodiments, the cellular adhesion agent can be a low- to mid-weight viscosity cellular adhesion agent. In some embodiments, a cellular adhesion agent can include, but is not limited to, carboxymethylcellulose (CMC). In certain embodiments, a low- to mid-weight viscosity cellular adhesion agent can be a low- to mid-weight viscosity carboxymethylcellulose. In some embodiments, carboxymethylcellulose can be present in an immunostimulatory composition disclosed herein at about 1% to about 20% (v/v).
Certain immunostimulatory compositions disclosed herein can include one or more TLR3 or TLR9 ligands known in the art. In some embodiments, a TLR9 ligand can include, but is not limited to, CpG oligonucleotides. In other embodiments, a TLR3 ligand can include, but is not limited to, poly-inosinic, poly-cytidylic acid (polyIC). In certain embodiments, combinations of TLR3 and TLR9 ligands can be present in the immunostimulatory compositions disclosed herein. In some embodiments, immunostimulatory compositions disclosed herein are more easily formulated than previous formulations for efficiency and ease of manufacture. In other embodiments, immunostimulatory compositions disclosed herein containing CpG oligonucleotides instead of non-coding plasmid DNA can be even more immuno-potent for improved non-specific immune stimulation when introduced to a subject. In accordance with these embodiments, the immunostimulatory compositions include about 10 μg to about 200 μg TLR ligand or mixture of TLR ligand per milliliter. In certain embodiments, cationic liposomes can be present at about 1.0 mM to about 20 mM concentration in an immunostimulatory composition disclosed herein. In certain alternative embodiments, the immunostimulatory composition can further include an antigen. In some embodiments, the antigen can be a protein antigen. In other embodiments, the antigen can be derived from a virus, bacterium, prion, fungus, a toxin, a tumor-related antigen or other protein or non-protein antigen.
In certain embodiments, methods for inducing an innate immune response in a subject are disclosed. In certain embodiments, methods are disclosed for inducing an innate immune response in a subject having an infection or other health condition. In accordance with these embodiments, methods can include, but are not limited to, providing to a subject an effective amount of an immunostimulatory composition disclosed herein. In certain embodiments, the immunostimulatory composition can include: a composition including, but not limited to, (a) cationic liposomes; (b) one or more TLR ligands (e.g. a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands); and/or (c) a cellular adhesion agent.
In certain embodiments, the subject can be a human, a pet, livestock, other animal, a bird, or a fish. In other embodiments, the immunostimulatory composition can be provided to an animal prior to and/or during boarding. In even some embodiments, an immunostimulatory composition disclosed herein can be used to treat a microbial infection. In some embodiments, a microbial infection can be from a virus, a bacterium, a fungus, prion or protozoan. In some embodiments, the microbial infection causes a respiratory infection.
In some embodiments, an immunostimulatory composition disclosed herein can include a TLR3 and a TLR9 ligand including, but not limited to a polynucleotide represented by plasmid DNA and poly(I:C). In other embodiments, an immunostimulatory composition disclosed herein can include a plasmid DNA represented by a polynucleotide represented by SEQ ID NO. 1 and poly(I:C). In other embodiments, an immunostimulatory composition disclosed herein can include a TLR3 and a TLR9 ligand including, but not limited to CpG oligonucleotide and poly(I:C). In some embodiments, an immunostimulatory composition disclosed herein can include a CpG oligonucleotide represented by a polynucleotide represented by SEQ ID NO. 3 or 4 or similar sequence and poly(I:C).
In other embodiments, methods for inducing an immune response to an antigen in a subject are disclosed including, but not limited to, providing to the subject a composition including: (a) cationic liposomes; (b) a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands; (c) a cellular adhesion agent and the antigen. According to certain aspects, the composition can be administered to treat or prevent a condition in a subject, by administering the composition orally, intranasally, nasally, topically, intradermally, topically, intravenously, subcutaneously, intra-vaginally, by uterine or intra-mammary injection, by aerosol delivery or other suitable means of administration.
In some embodiments, adhesive agents disclosed herein can include one or more of, carboxymethyl cellulose, chitosan, a polyglycol, or a hyaluronic acid like agent. In certain embodiments, the immunostimulatory composition of use to treat a subject can include a liposome and combination TLR (e.g. TLR 3 and TLR9 ligand) compositions along with and a low molecular weight/low viscosity adhesive agent. In some embodiments, adhesive agent can include, but is not limited to, low molecular weight carboxymethylcellulose (CMC). In some embodiments, the CMC is low to medium CMC is less than about 1500 centipoise (cps) or less than about 1000 cps or less than about 750 cps, or less than about 400 cps, or less than about 250 cps or less than about 150 cps, or less than about 100 cps or about 50 to 200 cps. In other embodiments, high viscosity CMC can be used in compositions disclosed herein having about 1500 to about 3000 centipoise (cps).
In certain embodiments, immunostimulatory compositions disclosed herein are formulated for prolonged administration to a subject, reducing frequency of application to a site of infection and/or condition. In some embodiments, immunostimulatory formulations disclosed herein are specifically designed for topical application. In some embodiments, an immunostimulatory formulation of use for topical administration includes cationic liposomes, a mixture of TLR3 and TLR9 such as CpG oligonucleotides and poly(I:C) and at least one adhesive agent. In accordance with these embodiments, an essentially liquid immunostimulatory formulation disclosed herein further comprises an adhesion agent. In some embodiments, a CMC solution can be combined with an essentially liquid immunostimulatory formulation disclosed herein at a predetermined ratio. In accordance with these embodiments, these formulations will have increased viscosity to a gel-like consistency to increase contact time in an affected area (e.g. skin, eye, nasal passage way or other affected or infected area). In other embodiments, a CMC solution can include a low molecular weight CMC as one component to the immunostimulatory compositions disclosed herein in order to increase viscosity of a composition disclosed herein while permitting intradermal and intravenous or systemic delivery to a subject in need thereof.
In some embodiments, an immunostimulatory composition disclosed herein can be provided to the subject within hours to within 24 hours prior to the risk of exposure and/or after exposure, during onset of an infection, during an infection or during chronic infection. In accordance with these embodiments, immunostimulatory compositions disclosed herein are capable of inducing a local, non-specific immune response at a site of administration. In certain embodiments, immunostimulatory compositions disclosed herein can be administered by any means known in the art to the subject. In certain embodiments, immunostimulatory compositions disclosed herein can be administered to the reproductive tract, the gastrointestinal tract, the mammary gland, to gills, to air sacs, to eyes, to ears, intranasally, intraocularly, intradermally, topically to the subject in need of such a treatment. In yet other embodiments, the composition can be administered without the concurrent administration of a vaccine or other standard or known agent for the treatment of or for reducing onset of a condition.
In other embodiments, an immunostimulatory composition can be used to treat a subject having an adverse eye condition. In accordance with these embodiments, an eye condition can include but are not limited to, an infection, a tumor, an eye injury, chronic wound or ulcer or similar condition of the eye thereof. In certain embodiments, the infection can be caused by a Herpesvirus or other microorganism capable of causing an eye infection. In some embodiments, the infection can be a chronic viral infection of the eye of a subject. In other embodiments, the infection can involve the adnexa of the eye such as the conjunctiva and sclera, which can further involve cancer (e.g. squamous cell carcinoma) or chronic infection (e.g., herpesvirus infection, mycoplasma infection, calicivirus infection). In other embodiments, an immunostimulatory composition disclosed herein can be used to treat cancer of the eye.
While multiple embodiments are disclosed, still other embodiments of the disclosure will become apparent to those skilled in the art from the following detailed description, which shows and describes illustrative embodiments of the disclosed apparatus, systems and methods. As will be realized, the disclosed apparatus, systems and methods are capable of modifications in various obvious aspects, all without departing from the spirit and scope of the disclosure. Accordingly, the drawings and detailed description are to be regarded as illustrative in nature and not restrictive.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as understood by one of ordinary skill in the art to which the invention belongs.
As used herein, the term “effective amount” can refer to at least an amount effective, at dosages and for periods of time necessary, to achieve the desired result, e.g., enhance innate immune response, an enhanced immune response to an antigen. An effective amount can be provided in one or more administrations.
As used herein, the term “subject” or “individual” or “patient” refers to the target, e.g. human or an animal. A subject disclosed herein can be a vertebrate, such as human or other mammal, a fish, a bird, a reptile, or an amphibian. Alternatively, the subject can be a human (e.g. an adult, adolescent, young adult, child, infant or fetus), non-human primate, horse, pig, rabbit, dog, sheep, goat, cow, cat, guinea pig, deer, elk, fox, coyote, wolf, rodent. In one aspect, the subject is a mammal. e.g., a human, or a companion animal (e.g., dog, cat, rodent, rabbit, etc.), a sport animal (e.g., horse, dog, cow, pig, etc.), a farm or food animal (e.g., pig, cow, sheep, goat, etc.), livestock (e.g., donkeys, goats, guinea pigs, sheep, cattle, llamas, etc.), wild animal, or any other animal, or to a bird (e.g., chicken, turkey, duck) or other non-mammalian species such as farm-reared fish, or other species such as reptiles or amphibians. In certain embodiments, the subject can be an adult or a young or immature animal or unborn animal.
As used herein, the singular form “a”, “an”, and “the” can include plural references unless indicated otherwise.
As used herein, “about” can include a value or parameter herein refers to the usual error range for the respective value readily known to the skilled person in this technical field. In particular embodiments, reference to about refers to a range within 10% higher or lower than the value or parameter, while in other embodiments, it refers to a range within 5% or 20% higher or lower than the value or parameter. Reference to “about” a value or parameter herein includes (and describes) aspects that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X.”
As used herein, the term “modulating” can mean changing, and can include positively modulating, such as increasing, enhancing, inducing or stimulating, as well as negatively modulating such as decreasing, inhibiting or reducing, typically in a statistically significant or a physiologically significant amount as compared to a control. An increased, stimulated or enhanced amount is typically a statistically significant amount, and can include an increase that is 1.1, 1.2, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30 or more times (e.g., 500, 1000 times) A decreased, inhibited or reduced amount can be a statistically significant amount, that can include a 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%), 80%, 85%, 90%, 95%, or 100% decrease in the amount produced by a control of no treatment as described herein or by a control treatment, including all integers in between.
As used herein, the term “adjuvant” can mean its conventional meaning, for example, the ability to enhance the immune response to a particular antigen or to enhance the immune response in general without an antigen. Such ability is manifested by a significant increase in immune-mediated protection. An enhancement of humoral immunity is typically manifested by a significant increase (usually >10%) in the titer of antibody raised to the antigen. Similarly, enhancement of cellular immunity is typically manifested by a significant increase (usually >10%) in the number of responding CD8+ or CD4+ T cells.
As used herein, the term “Poly(I:C)” “PolyIC” (polyinosinic-polycytidylic acid) is an agent that can be recognized by TLR3. This recognition leads to induction and activation of NF-kB and the production of certain cytokines. Poly(I:C) is composed of a strand of poly(I) annealed to a strand of poly(C). The size of the strands can vary. (e.g. InvivoGen and other manufacturers provide poly(I:C) with at least 2 different sizes: Poly(I:C) (HMW) with a high molecular weight has an average size of about 1.5 kb to about 8.0 kb, and Poly(I:C) (LMW) with a low molecular weight of about 0.2 kb to about 1 kb.
As used herein, the term “CpG oligodeoxynucleotides” (CpG ODN; CpG oligos; CpG oligonucleotides) can mean short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide (“C”) followed by a guanine triphosphate deoxynucleotide (“G”). The “p” refers to the phosphodiester link between consecutive nucleotides, although some ODN have a modified phosphorothioate (PS) backbone instead. In certain embodiments, CpG oligonucleotides are fully phosphorylated, partially phosphorylated or unphosphorylated. In certain embodiments, when these CpG motifs are unmethylated, they act as immunostimulants CpG motifs are considered pathogen-associated molecular patterns (PAMPS) due to their abundance in microbial genomes but their rarity in vertebrate genomes. The CpG PAMP is recognized by the pattern recognition receptor (PRR) Toll-Like Receptor 9 (TLR9), in mammals and avian and fish species.
As used herein, “PCT-01” or “MiM” refer to a complex solution that includes cationic liposomes, non-coding plasmid DNA, polyinosinic-polycytidylic acid, and low and/or medium viscosity (and in certain embodiments, molecular weight) adhesion agents of carboxymethylcellulose in a diluent for in vitro and in vivo studies.
As used herein “Non-coding plasmid DNA” can include bacterial replication elements in a circular arrangement. The DNA in plasmids can act as an immunostimulant recognized for example, by the pattern recognition receptor (PRR) Toll-Like Receptor 9 (TLR9), which is expressed in mammals and avian species. In addition, these non-coding plasmids can be engineered to overexpress CpG motifs. In the instant case, the plasmid does not code for any known mammalian genes, and instead codes for several “islands” of CpG motifs (oligonucleotides) engineered into the plasmid to increase its immune stimulatory properties.
In the following sections, various exemplary compositions and methods are described in order to detail various embodiments of the invention. It will be obvious to one skilled in the art that practicing the various embodiments does not require the employment of all or even some of the specific details outlined herein, but rather that concentrations, times and other specific details may be modified through routine experimentation. In some cases, well known methods, or components have not been included in the description.
In some embodiments, the instant disclosure relates, to improved immunostimulatory compositions, which may be used to induce a non-specific, protective mucosal immune response or other non-specific immune response in a subject in order to reduce onset of, or treat a health condition. In some embodiments, the improved immunostimulatory compositions can be used to reduce the onset of, or treat a microbial infection, cancer or other health condition. In some embodiments, immunostimulatory compositions having a TLR9 ligand of CpG oligonucleotides in a combination formulation with a TLR3 ligand (e.g. poly(I:C)), an adhesion agent and a mixture of cationic liposomes can have about 10%, 20%, 30%, 40%, 50%, 1.5 times, 2 times or higher immunostimulatory potency than other formulations. In other embodiments, a comparative formulation can include a substitution of CpG oligonucleotides for other TLR9 ligands such as non-coding plasmid DNA or other TLR9 ligands for improved immunostimulatory effect and induction of immunostimulatory molecules for an improved non-specific immune response in a subject.
In certain embodiments, compositions disclosed herein include improved immunostimulatory compositions. In accordance with these embodiments, immunostimulatory or immunostimulatory compositions designed to more effectively stimulate local immune responses at mucosal and epithelial surfaces of a subject are disclosed. In certain embodiments, the immunostimulatory compositions disclosed herein can be used to stimulate local immune responses at mucosal surfaces such as the nasopharynx, upper respiratory tract or other lung compartment, eye, GI tract, and reproductive tract to reduce the risk of microbial infection or treat microbial infection or reduce the risk of or treat other health conditions in need of such a treatment. In accordance with these embodiments, these immunostimulatory compositions improve properties of a previously developed immunotherapeutic (e.g. cationic-liposome DNA complexes; CLDC). In some embodiments, immunostimulatory compositions disclosed herein have improved adhesion properties. For example, immunostimulatory compositions disclosed herein have improved properties, including but not limited to, adhesion to mucosal surfaces, increased potency of immune activation, and duration of immune activation.
In some embodiments, mucosal immune stimulation and adhesion technology disclosed herein provides significant improvement over previously disclosed CLDC technology, which in itself is a potent immune stimulant. In certain embodiments, immunostimulatory compositions disclosed herein provide for improved induction of mucosal immune responses in order to treat a health condition.
Some embodiments disclosed herein include immunostimulatory compositions including at least one of (a) cationic liposomes, at least one (b) toll like receptor (TLR) or mixture thereof, and at least one (c) cellular adhesion agent. Toll-like receptors (TLRs) are conserved pattern recognition receptors expressed on multiple types of cells, including monocytes, dendritic cells, B cells, and macrophages, and play a vital role in modulation of the innate immune system. In certain embodiments, the TLRs include but are not limited to, TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, and TLR10. In other embodiments, TLRs of use in immunostimulatory compositions disclosed herein include, but are not limited to, TLR3 and TLR9 ligands. In other embodiments, cationic liposomes can include, but are not limited to, a mixture of cationic lipid and non-charged lipids. In accordance with these embodiments, a mixture of cationic lipid and non-charged lipids can include a mixture of DOTAP and cholesterol. In certain embodiments, DOTAP and cholesterol can be about a 1:1 molar ratio or about a 2:1 or about a 1:2 or about 1.5:1 or about 1:1.5 or 1:3 or 3:1 or similar ratio. In some embodiments, the mixture of cationic lipid and non-charged lipids can further include at least one of, non-coding plasmid DNA and polyI:C. In other embodiments, the non-coding plasmid DNA can include a polynucleotide represented by the nucleic acid sequence, SEQ ID NO. 1. In some embodiments, the mixture of cationic lipid and non-charged lipids can further include at least one of, CpG oligonucleotides and polyI:C. In other embodiments, the CpG oligonucleotides can include a polynucleotide represented by the nucleic acid sequence, SEQ ID NO. 3 or 4 or similar sequence. In yet other embodiments, the mixture of cationic lipid and non-charged lipids can include CpG oligonucleotides (or plasmid DNA) and poly(I:C) in about a 1:1 ratio (by weight) or about a 2:1 or about a 1:2 or about a 1.5:1 or about a 1:1.5 or similar ratio. In certain embodiments, the cellular adhesion agent in an immunostimulatory composition disclosed herein can be a low- to mid-weight viscosity adhesion agent. In some embodiments, the low- to mid-weight viscosity adhesion agent is carboxymethylcellulose (CMC). In some embodiments, carboxymethylcellulose can be present in an immunostimulatory composition disclosed herein at about 1% to about 20% (v/v) or about 5% to about 15% (v/v). Certain immunostimulatory compositions disclosed herein can include complexes of the cationic liposomes and any TLR3 and/or TLR9 ligands known in the art. In other embodiments, complexes disclosed herein can include about 10 μg to about 200 μg TLR ligand per milliliter cationic liposomes. In some embodiments, cationic liposomes can be present at about 1 to about 20 mM concentration in an immunostimulatory composition disclosed herein. In some alternative embodiments, immunostimulatory composition can further include an antigen. In some embodiments, the antigen can be a protein antigen. In other embodiments, the antigen can be derived from a virus, bacterium, prion, fungus, a toxin, a tumor-related antigen or other protein or non-protein antigen. In accordance with these embodiments, immunostimulatory compositions disclosed herein in combination with a targeted antigen can be used to induce an improved innate immune response compared to an antigen without an immunostimulatory composition of the instant application against the antigen. In certain embodiments, an enhanced innate immune response includes a local or regional response at the site of introducing the immunostimulatory compositions.
In certain embodiments, non-specific immunostimulatory compositions of use to induce a non-specific immune response in a subject can be referred to as LTC 1.0. In accordance with these embodiments, LTC 1.0 can include a disaccharide containing buffer (e.g. about 5% to about 20% sucrose or other disaccharide) at pH of about 6.8 to about 7.6 as a diluent; a DOTAP: cholesterol liposome solution (1:1, 1:2, 2:1, 3:1, 1:3 or similar ratio); poly(I:C) (e.g. about 10 to about 150 μs; about 5 to 100 μs; about 50 μs) and plasmid DNA (e.g. about 10 to about 150 μs; about 5 to 100 μs; about 50 μs) and carboxymethylcellulose (e.g. low molecular weight, 0.01% to 10% stock solution or about 1.0%).
In certain embodiments, non-specific immunostimulatory compositions of use to induce a non-specific immune response in a subject can be referred to as LTC 2.0. In accordance with these embodiments, LTC 1.0 can include a disaccharide containing buffer (e.g. about 5% to about 20% sucrose or other disaccharide) at pH of about 6.8 to about 7.6 as a diluent; a DOTAP:cholesterol liposome solution (1:1, 1:2, 2:1, 3:1, 1:3 or similar ratio); poly(I:C) (e.g. about 10 to about 150 μs; about 5 to 100 μs; about 50 μs) and CpG oligonucleotides (e.g. about 10 to about 150 μs; about 5 to 100 μs; about 50 μs) and carboxymethylcellulose (e.g. low molecular weight, 0.01% to 10% stock solution or about 1.0%).
In other embodiments, methods for inducing an innate immune response in a subject are disclosed. In certain embodiments, methods are disclosed for inducing an innate immune response in a subject having an infection or other condition. In accordance with these embodiments, methods can include, but are not limited to, providing to a subject and immunostimulatory composition disclosed herein. In certain embodiments, the immunostimulatory composition disclosed herein can include: (a) at least one cationic liposome; (b) one or more TLR ligands (e.g. a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands); and/or (c) one or more cellular adhesion agent.
In some embodiments, a health condition is an infection. In accordance with these embodiments, the infection can be as a result of a virus, bacterium, fungus, prion or protozoan infection. In certain embodiments, the infection includes a respiratory infection. In some embodiments, the immunostimulatory compositions disclosed herein can be administered to the lungs of the subject to treat the infection. In accordance with these embodiments, an immunostimulatory composition disclosed herein can include (a) at least one cationic liposome; (b) one or more TLR ligands (e.g. a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands); and/or (c) one or more cellular adhesion agent.
In some embodiments, an immunostimulatory composition disclosed herein can be provided to the subject prior to the risk of or exposure to a microorganism or at the time of exposure to a microorganism and/or within 24 hours to a week or more after exposure, during early onset of clinical signs of an infection, or during chronic infection. In accordance with these embodiments, immunostimulatory compositions disclosed herein are capable of inducing a local, non-specific immune response at a site of administration or at the delivery site after administration (e.g. the lungs) or at the site of infection. In certain embodiments, immunostimulatory compositions disclosed herein can be administered to the subject at the site of a wound or infected tissue, or administered to induce a systemic non-specific immune response to treat a subject. In certain embodiments, immunostimulatory compositions disclosed herein can be administered to the reproductive tract, the gastrointestinal tract, the mammary gland, to gills, to air sacs, to eyes, to ears, and to the nose of a subject in need of such a treatment. In yet further aspects, the composition can be administered without the concurrent administration of a vaccine or other known agent for the treatment or reducing onset of a condition.
Further, disclosed herein are embodiments directed to methods for inducing an immune response to an antigen in a subject, including providing to the subject a composition including: (a) at least one cationic liposome; (b) a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands; and (c) at least one cellular adhesion agent; and optionally, an antigen. In other embodiments, an immunostimulatory composition disclosed herein can be administered to the eye of a subject. In other embodiments, a composition including: (a) at least one cationic liposome; (b) a mixture of toll like receptor 3 (TLR3) and toll like receptor 9 (TLR9) ligands; and (c) at least one cellular adhesion agent can further include a high viscosity and/or high molecular weight (HMW) cellular adhesion agent. In accordance with these embodiments, immunostimulatory compositions further including a high viscosity and/or high molecular weight adhesion agent can be administered to a subject having a health condition in need of non-specific immune stimulation such as to treat an infection of any tissue or location in a subject. In certain embodiments, the infection is a chronic or non-healing infected tissue.
In other embodiments, a health condition is an eye condition. In accordance with these embodiments, the eye condition can include but is not limited to, an infection, a tumor, an eye injury, chronic wound or ulcer or similar condition of the eye thereof. In accordance with these embodiments, the eye condition can include a condition that affects the cornea or retina or other component of the eye. Certain embodiments of the invention can include administering an immunostimulatory composition disclosed herein to the eye of a subject to reduce incidence of blindness or injury to the eye or to treat an infection of the eye. In some embodiments, an infection of the eye can be caused by a virus, bacterium, fungus, prion or other microorganism. In certain embodiments, the infection can be caused by a Herpes virus or other microorganism capable of causing an eye infection. In some embodiments, the eye condition can include an infection of the cornea, adverse condition of the cornea or outer service of the eye.
In some embodiments, immunostimulatory compositions disclosed herein of use to treat a subject can provide broad spectrum activity of increased duration, reducing frequency of treatment and having reduced side effects such as irritation and inflammation. In certain embodiments, the immunostimulatory compositions disclosed herein can reduce the incidence of irritation and inflammation as well as treat chronic eye conditions with improved outcomes.
In other embodiments, immunostimulatory compositions disclosed herein are formulated for prolonged administration reducing frequency of application to a site of infection and/or condition. In some embodiments, immunostimulatory formulations disclosed herein are designed for topical administration. In certain embodiments, immunostimulatory formulations disclosed herein are designed for administering as a drop such as a viscous drop or as a topical cream or lotion. In some embodiments, an immunostimulatory formulation of use for topical administration includes cationic liposomes, a mixture of TLR3 and TLR9 ligands and an adhesive agent. In accordance with these embodiments, an essentially liquid immunostimulatory formulation disclosed herein (e.g. CLDC plus CMC of low to mid viscosity: MiM) can further include at least one high viscosity adhesion agent. In yet other embodiments, a TLR3 ligand can include CpG oligonucleotides and TLR9 ligand can include polyIC. In certain embodiments, a high viscosity or high molecular weight adhesion agent can include, but is not limited to, carboxymethylcellulose (CMC). Other suitable high viscosity and/or high molecular weight adhesion agents include, but are not limited to, dextrans, hyaluronic acid, chondroitin sulfate, petrolatum, mineral oil, and/or lanolin. In other embodiments, a high viscosity and/or high molecular weight adhesion CMC solution is combined with an essentially liquid immunostimulatory formulation disclosed herein at a predetermined ratio. In accordance with these embodiments, these formulations will have increased viscosity to a gel-like consistency to increase contact time in an affected area (e.g. the eye, nasal passageway or other mucosal surface) in order to increase duration of exposure and improve outcome.
In certain embodiments disclosed herein, combination treatments are contemplated. In accordance with these embodiments, immunostimulatory compositions disclosed herein (e.g. CLDC plus CMC of low to mid viscosity: MiM) having at least one high molecular weight and/or high viscosity adhesion agent included in the formulation can be used in combination with standard treatments for infections, chronic wounds or ulcers and tumors to obtain improved outcomes. In certain embodiments, immunostimulatory compositions and formulations disclosed herein can be used before, during or after standard treatment regimens in order to improve outcome. In some embodiments, immunostimulatory compositions disclosed herein can be used to reduce cost of treatment and reduce the risk of recurrence of the health condition such as infection.
In some embodiments, the immunostimulatory compositions of the present invention can be used to induce non-specific immune responses in humans, and pets such as dogs, cats, rabbits; in livestock such as cattle, swine, in horses or other performance animals and birds, such as chickens, turkeys and other birds and fish. In certain embodiments, compositions disclosed herein can be used to treat or reduce the risk of onset of a viral, bacterial, fungus, prion or protozoan infection. In some embodiments, infections of a subject contemplated herein can be a respiratory, ear, eye, sinus, skin, scalp, oral, throat infection, as well as infections of the reproductive or gastrointestinal (GI) tract.
In certain embodiments, compositions disclosed herein can be administered as a liquid by the intranasal and oropharyngeal routes to humans and other mammals (e.g., dogs, cats, cattle, horses, swine, sheep, goats, buffalo poultry) prior to exposure or after exposure to a pathogen. In certain embodiments, animals can be administered an immunostimulatory composition disclosed herein about 24 hours prior to exposure to a pathogen (e.g., shipping animals to feedlots, boarding facilities, veterinary visits or rearing facilities), or within 7 days following exposure to a pathogen and optionally, daily, weekly, bi-weekly or by other regimen while in the facility and for a time after leaving a facility, if desired. In some embodiments, compositions disclosed herein can be used to induce local immune responses in order to reduce the risk of onset of an infection, such as against a virus or bacteria. In some embodiments, the composition may be administered to an animal in a shelter boarding facility to induce an enhanced immune response to a respiratory infection. In some embodiments, a respiratory infection could be one that occurs in a cat or a dog such as an upper respiratory infection due to exposure to a microorganism. It is contemplated herein, that compositions disclosed herein can be of use to a subject in quarantine or other holding facility to reduce the risk of exposing others to a potential infection.
In certain embodiments, immunostimulatory compositions disclosed herein can be used to treat a subject for, or reduce the risk of onset in a subject of, a viral infection by inducing an enhanced immune response to a respiratory virus such as rhinovirus, influenza virus, adenovirus, or the like. In certain embodiments, immunostimulatory compositions disclosed herein can be used to treat a subject for or reduce the risk of onset in a subject of, a bacterial infection such as Staphylococcus, Pneumococcal, Streptococcus or other bacterial infection.
In a related embodiment, cattle that are shipped to feedlots could be administered an immunostimulatory composition disclosed herein by intranasal or other rapid administration, before or upon arrival to the facility. It is contemplated that the treatment can be repeated at predetermined intervals such as daily or weekly or by bi-monthly intervals as appropriate.
In another embodiment, poultry in intensive husbandry settings (e.g., broiler operations) that are exposed to pathogens or at risk of exposure to pathogens can be treated with immunostimulatory compositions disclosed herein throughout the building by exposure to an aerosol mist generated by an aerosol generator carried as a backpack by facility personnel.
In another embodiment, fish in for example, fish farms or ponds at risk of infection could be collected into smaller treatment tanks, and the composition can be introduced to the water in the tanks so all the fish would be treated via uptake by the gills or other mucosal surfaces.
In certain embodiments, the immunostimulatory composition may be used to treat a human. In accordance with these embodiments, the composition can be administered by any method known in the art. In certain embodiments, the immunostimulatory compositions can be administered to a human intranasally or to the eye by a dropper or ointment or other appropriate form. In certain embodiments, the immunostimulatory composition can be administered as a liquid or spray using a spray bottle, eye dropper, intranasal device or similar device. In other embodiments, humans at risk of contracting a viral infection or being exposed to a bacterial infection (e.g., during airline travel, holiday gatherings, classrooms) can administer immunostimulatory compositions disclosed herein prior to the encounter or prior to traveling and then one day to about 7 days to within 14 days after exposure or suspected exposure.
In other embodiments, improved immunostimulatory compositions are disclosed. In accordance with these embodiments, the improved or enhanced immunostimulatory compositions can be used as adjuvants, alone or in combination with antigens in, for example, vaccines or other immunostimulatory agent. In certain embodiments, immunostimulatory compositions disclosed herein include an improved liposomal vaccine adjuvant with greater lymph node trafficking ability for greater vaccine adjuvant activity. In certain embodiments, by combining an adhesive agent (e.g., carboxymethylcellulose) with a cationic liposome-TLR agonist complex, migration of vaccine antigens to draining lymph nodes is enhanced, resulting in enhanced immune responses, as well as, enhance immune responses to antigens (e.g. protein antigens). In certain embodiments, immunostimulatory compositions disclosed herein provide improved immunostimulatory properties of a previously developed immunotherapeutic (e.g. cationic-liposome DNA complexes; CLDC); for example, with respect to vaccine adjuvant properties and immunological responses to administration of a vaccine.
In certain embodiments, immunostimulatory compositions disclosed herein can include an immunostimulatory composition for application to a subject. In accordance with these embodiments, the immunostimulatory compositions can include, but are not limited to, the following components: cationic liposomes including at least one cationically-charged lipid in a predetermined ratio with cholesterol; one or more TLR ligand, such as TLR3 and/or TLR9 ligands or agonists (e.g. TLR ligands); for example, including non-coding plasmid DNA or CpG oligonucleotides (TLR9 agonist) and/or polyinosinic-polycytidylic acid (TLR3 agonist); at least one cellular adhesion agent (e.g., carboxymethylcellulose, or chitosan, polyglycol, or hyaluronan) and further comprising a cell adhesion agent of low to mid molecular weight or viscosity. In certain embodiments, the composition includes cationic liposomes. In certain embodiments, the cationic liposomes include one or more of DOTAP and cholesterol. In other embodiments, the TLR9 ligand includes CpG oligonucleotides represented by SEQ ID NO. 3 or 4 or the like. In certain embodiments, the TLR3 ligand includes poly IC. In accordance with these embodiments, the polyIC can be a high molecular weight polyIC. In accordance with these embodiments, the polyIC can be about 0.5 kb to about 15 kB or about 1.5 kb to about 8.0 kb. In some embodiments, the adhesion agent can be a carboxymethylcellulose agent. In some embodiments, the adhesive agent can be a 0.5% to a 20.0% v/v or about 5% to about 20% v/v or about 5% to about 15% or about 10% v/v. In other embodiments, the adhesion agent is CMC and the CMC solution can be about a 0.5% to about a 5.0% solution or a 0.5% to a 3% solution.
In some embodiments, the immune-stimulating composition includes, but is not limited to, 1) cationic liposomes of DOTAP and cholesterol; 2) CpG oligonucleotides represented by a polynucleotide represented by SEQ ID NO: 3 or 4 or the like; 3) polyIC having a size of about 1.5 to about 8 kb and 4) carboxymethylcellulose having a concentration of about 5% to about 15% v/v or about 10% v/v (referred to as Liposome-TLR3+9 (LTC)). In accordance with these embodiments, an LTC immune therapeutic includes, but is not limited to, synthetic TLR9-stimulatory CpG oligonucleotides and synthetic TLR3 double stranded RNA molecules such as polyI:C, complexed to cationic liposomes and carboxymethylcellulose. In certain embodiments, this immune therapeutic is designed to activate innate immune responses in multiple different mammalian species, following topical or parenteral administration. Screening studies demonstrate innate immune activating properties across multiple species, and in multiple different cell types. In certain embodiments, the immunostimulatory essentially liquid composition becomes a viscous gel-like consistency for administration to the subject to prolong exposure to the composition and prolong non-specific immunostimulatory effects.
Embodiments disclosed herein provide for novel immunostimulatory compositions. In certain embodiments, these immunostimulatory compositions are used to induce a non-specific immune response in a subject.
In other embodiments, the immunostimulatory compositions can include, but are not limited to, the following components: cationic liposomes including at least one cationically-charged lipid in a predetermined ratio with cholesterol; one or more TLR ligand, such as TLR3 and/or TLR9 ligands or agonists (TLR ligands), including non-coding plasmid DNA and/or CpG oligonucleotides (TLR9 agonist) and polyinosinic-polycytidylic acid (TLR3 agonist); and at least one cellular adhesion agent (e.g., carboxymethyl cellulose, or chitosan, polyglycol, or hyaluronan). In other embodiments, the immunostimulatory compositions can further include a high molecular weight/high viscosity adhesion molecule such as HMW/high viscosity CMC or other HMW/high viscosity adhesion agents (e.g. chitosan, polyglycol, poloxamer agent or hyaluronan).
In some embodiments, immunostimulatory compositions includes both a CLDC and a cellular adhesion agent of low and/or medium molecular weight. In certain embodiments, the immunostimulatory composition includes both a CLDPC and a cellular adhesion agent of low and medium-high molecular weight.
In one embodiment, the TLR ligand can be a cationic liposome combined with a TLR9 agonist (e.g. either plasmid DNA (e.g., non-coding plasmid DNA), or CpG oligos)), referenced herein in certain embodiments as a CLDC adjuvant. In one embodiment, the TLR ligand is a cationic liposome DNA-pIC complex (CLDPC). According to certain exemplary embodiments, the TLR9 agonist can be a non-coding plasmid represented by SEQ ID NO. 1. In accordance with these embodiments, the plasmid (See for example,
In certain embodiments, the immunostimulatory compositions of the present invention can elicit both a cell-mediated immune response and a humoral immune response when administered to a subject. In some embodiments, these immune responses can induce prolonged exposure to antibodies as well as an enhanced T cell-mediated immune response. In some embodiments, the enhanced T-cell response can include and enhanced CD4 and/or CD8 T-cell response. In certain embodiments, the disclosed CLDC adjuvant primarily elicits a Th1 response. In some embodiments, the TLR ligand is prepared with a CLDC adjuvant and/or CLDPC adjuvant capable of eliciting an enhanced and effective cell-mediated immunity. In certain embodiments, the immunostimulatory compositions can include other adjuvants capable of eliciting and enhanced Th1 immune response.
In some embodiments, the TLR ligand includes, but is not limited to, cationic liposomes complexed to non-coding plasmid DNA (CLDC), as this adjuvant is particularly effective in eliciting T cell (e.g. such as CD8 and CD4) responses. In other embodiments, the CLDC adjuvant can be prepared using cationic liposomes combined with CpG oligos. In some embodiments, the CLPDC can include cationic liposomes complexed to polyI:C and plasmid DNA. In other embodiments, the complex includes cationic liposomes (e.g., DOTAP) in a 1:1 to 1:2 molar ratio with cholesterol, e.g., formulated as small unilamellar vesicles in dextrose or sucrose solution, and polyI:C and/or plasmid DNA (e.g., non-coding DNA). When both are present, in certain embodiments, the polyI:C and plasmid DNA may be present in a ratio of 1:2 to 2:1, e.g., 1:1 (by weight). In certain embodiments, the complex contains about 10 μg to about 500 μg, about 50 μg to about 200 μg, or about 100 μg total of polyI:C and/or DNA per 1 ml liposomes or other volume of liposomes. In some embodiments, the liposome concentration can be from about 0.1 to about 20.0 mM or about 5.0 to about 15.0 mM or about 10.0 mM. In other embodiments, the cationic liposomes can include a cationic lipid (e.g., DOTAP or DOTIM or similar cationic lipid) mixed in a 1:1 or 2:1 or 1:2 or 3:1 or 1:3 molar ratio with cholesterol and rehydrated to produce liposomes in the range of about 100 to about 350, to about 150 to about 300 to about 250 nm diameter. In certain embodiments, any of the CLDC and CLPDC adjuvants can include a cellular adhesive agent. In some embodiments, the cellular adhesion agent is a low to medium molecular weight cell adhesion agent. In other embodiments, the cellular adhesion agent is a high molecular weight/high viscosity adhesion agent. In certain embodiments, the cell adhesion agent can be carboxymethylcellulose (CMC). In certain embodiments, the CLDC adjuvant can include cationic liposomes (e.g., DOTAP and cholesterol (10 mM), 1:2 to 2:1 ratio or about 1:1 ratio), and non-coding plasmid DNA or CpG oligonucleotides (e.g. about 10 μg/ml to about 500 μg/ml or about 10 μg/ml to about 200 μg/ml, or about 50 μg/ml). In some embodiments, the CLDC adjuvant can include cationic liposomes (e.g., DOTAP and cholesterol, 1:1 ratio), and non-coding plasmid DNA or CpG oligonucleotides (50 μg/ml). In other embodiments, the immunostimulatory compositions can include both a CLDC adjuvant and carboxymethylcellulose (CMC) at about 1.0% to about 20%, about 2.0% to about 15%, about 2.5% to about 10%, about 5.0% to about 10% or about 10% or about 5% v/v. In certain embodiments, the CLPDC adjuvant can include cationic liposomes (e.g., DOTAP and cholesterol, 1:2 to 2:1 ratio or about 1:1 ratio), non-coding plasmid DNA or CpG oligonucleotides (about 10 μg/ml to about 500 μg/ml or about 10 μg/ml to about 200 μg/ml, or about 50 μg/ml), and synthetic pIC (about 10-500 μg/ml or about 10-200 μg/ml or about 50 μg/ml). In some embodiments, the immunostimulatory composition can include, but is not limited to, cationic liposomes (e.g., DOTAP and cholesterol, at a 3:1, 1:3, 2:1, 1:2, 1:1 ratio), non-coding plasmid DNA or CpG oligonucleotides (about 10.0 to about 100.0 μg/ml), and synthetic pIC (about 50 μg/ml). In some embodiments, an LTC immunotherapeutic composition can include, but is not limited to, cationic liposomes (e.g., DOTAP and cholesterol, at a 3:1, 1:3, 2:1, 1:2, 1:1 ratio), CpG oligonucleotides (about 10.0 to about 100.0 μg/ml), and synthetic pIC (e.g. high molecular weight having an average size of about 0.5 to about 10 kb or about 1.5 to about 8.0 kb) and CMC (about 1.0 to about 20.0% v/v). In certain embodiments, the compositions includes both a CLPDC composition and carboxymethylcellulose (CMC) at about 1% to about 20%, about 2% to about 15%, about 2.5% to about 10%, about 5% or about 10% or about 5% v/v in the final composition.
In certain embodiments, the immunostimulatory composition or adjuvanted composition includes at least one cellular adhesion agent. In some embodiments, the at least one cellular adhesion agent enhances uptake of the composition by the mucosa or other tissue and cells exposed to the compositions and/or increases adhesion for prolonged exposure to the composition.
In some embodiments, the cellular adhesion agent can be carboxymethylcellulose, e.g. a low to mid-weight viscosity formulation and/or a high molecular weight cellular adhesion agent. Carboxymethylcellulose (CMC) or cellulose gum is a cellulose derivative with carboxymethyl groups (—CH2-COOH) bound to some of the hydroxyl groups of the glucopyranose monomers that make up the cellulose backbone. In certain embodiments, the CMC is a sodium salt derivative, sodium carboxymethyl cellulose. In some embodiments, CMC is present in the composition at about 0.1% to about 20%, about 1% to about 20% (v/v), 2% to 15%, 2.4% to 10%, 2.5% to about or about 5% (v/v). In some embodiments, low viscosity carboxymethylcellulose (CMC) agents can have a viscosity of a 4% solution in a diluent (e.g. water or PBS or other) at about room temperature (e.g. 25° C.) and can be 50-200 centipoise (cps). Viscosity is both concentration and temperature dependent. As the temperature increases, the viscosity decreases. As the concentration of these agents increases, the viscosity increases. In various embodiments, low, medium and high viscosity carboxymethylcellulose (CMC) are used in the compositions of the present invention. Low viscosity CMC is usually used in “thin” aqueous solutions. Medium viscosity CMC is usually used to make solutions that look like a syrup. In other embodiments, low viscosity CMC can have a molecular weight of about 50 to about 150; or about 50 to about 100 or about 90 kDa; a degree of polymerization of 400; a degree of substitution of 0.65-0.90 (6.5-9.0 carboxymethyl groups per 10 anhydroglucose units); and a sodium content of about 8% by weight. In certain embodiments, medium viscosity carboxymethylcellulose (CMC) can have a viscosity of a 2% solution in a diluent (e.g. water or PBS or other) at about room temperature (e.g. 25° C.) and can be 400-800 centipoise (cps). In certain embodiments, medium viscosity CMC can have a molecular weight of about 150 to about 350; about 200 to about 300 or about 250 kDa; a degree of polymerization of about 1100; and a degree of substitution of about 0.7 (approximately 7 carboxymethyl groups per 10 anhydroglucose units). In certain embodiments, the CMC is low molecular weight and can be about 2.5 to about 20% v/v or about 10% v/v of the composition.
In other embodiments, high viscosity cell adhesion agents are contemplated. In some embodiments, a high viscosity cell adhesion agent can include carboxymethylcellulose (CMC) having viscosity in about 1% solution in a diluent (e.g. water or PBS or other) at about room temperature (e.g. 25° C.) and can be from about 1500 to about 3000 centipoise (cps). In some embodiments, high viscosity CMC as used herein can be used to make a mixture that resembles a cream or lotion. In other embodiments, high viscosity CMC can have increased viscosity compared to low or medium viscosity CMC while still being capable of delivery to a subject by a dropper bottle but has viscous gel-like properties. In certain embodiments, low viscosity CMC can be used in “thin” aqueous solutions. In some embodiments, high viscosity CMC has a molecular weight of about 400 to about 1000, about 500 to about 900, about 600 to about 800, about 650 to about 750; or about 700 kDa; a degree of polymerization of about 3200; and a degree of substitution of about 0.65-0.85 (6.5-8.5 carboxymethyl groups per 10 anhydroglucose units). As used herein, a “poise” is a unit of viscosity based on a flow rate using the standard of water at 20° C. having a poise value of exactly 1 centipoise or one hundredth of a poise. One poise can be referred to as “P” in the following equation: 1P=(0.10 kg/meter)/sec=(1 g/cm)/sec.
In certain alternative embodiments, the cellular adhesion agent can be chitosan. In further alternative embodiments, the cellular adhesion agent can be hyaluronan. Hyaluronan, also known as hyaluronic acid, is a is an anionic, nonsulfated mucoid polysaccharide of biological origin. According to still further embodiments, the cellular adhesion agent is a polymer. As will be appreciated by those skilled in the art, suitable polymers in these embodiments are those with hydrophilic functional groups or those that bind to specific receptors on cell or mucus surface (e.g., lectins, thiolated polymers) or lipoid S100.
In certain embodiments, the cellular adhesion agent can be a propylene glycol. As used herein, “propylene glycol” or “PEG” is a polyether compound of general formula H—(O—CH2-CH2)n-OH. PEGs are also known as polyethylene oxides (PEOs) or polyoxyethylenes (POEs), depending on their molecular weight PEO, PEE, or POG, as used herein, refers to an oligomer or polymer of ethylene oxide. The three names are chemically synonymous, but PEG has tended to refer to oligomers and polymers with a molecular mass below 20,000 g/mol, PEO to polymers with a molecular mass above 20,000 g/mol, and POE to a polymer of any molecular mass. PEG and PEO are liquids or low-melting solids, depending on their molecular weights. Throughout this disclosure, the 3 names are used indistinguishably. PEGs are prepared by polymerization of ethylene oxide and are commercially available over a wide range of molecular weights from 300 g/mol to 10,000,000 g/mol. In certain embodiments, the PEG is water-soluble (amphiphilic or hydrophilic), non-toxic, and pharmaceutically inert. Suitable polymeric moieties include, but are not limited to, polyethylene glycols (PEG), homo- or co-polymers of PEG, a monomethyl-substituted polymer of PEG (mPEG), or polyoxyethylene glycerol (POG). Suitable PEG polymers will vary substantially by weights ranging from about 200 to about 60,000. In certain embodiments, PEGs having molecular weights from 200 to 2,000 or from 200 to 500 are used. Lower-molecular-weight PEGs are also available as pure oligomers, referred to as monodisperse, uniform, or discrete. PEGs are also available with different geometries: branched PEGs have three to ten PEG chains emanating from a central core group; star PEGs have 10 to 100 PEG chains emanating from a central core group; and comb PEGs have multiple PEG chains normally grafted onto a polymer backbone. PEGs can also be linear.
In other embodiments, the cellular adhesion molecule can be a surfactant. In some embodiments, the cellular adhesion molecule can be a high molecular weight/high viscosity surfactant (e.g. a poloxamer such as poloxamer 403 or 407 or similar).
In one embodiment, immunostimulatory compositions disclosed herein are prepared by combining complexes of cationic liposomes with DNA (e.g. CpG oligonucleotides) and/or pIC. In other embodiments, the adhesive agent (also referred to as the cellular adhesive agent) can be added to the combined complexes. In certain embodiments, an antigen can be added to the combined complex formulations containing the cellular adhesive agent. In yet other embodiments, a high molecular weight adhesive agent is added to the combined complexes containing the cellular adhesive agent to make a HMW/high viscosity adhesive agent immunostimulatory composition and used in a subject to induce an enhanced immune response, alone or in combination with other standards of care.
In other embodiments, the composition can be administered by a variety of mucosal routes of delivery, including intranasally, intradermally, topically, orally, inter-rectally, intra-vaginally, or by the intra-mammary or intra-uterine route, or by aerosol mist exposure, or by dilution in water (fish). Alternative routes of delivery include parenterally, e.g., intravenously, subcutaneously, intraperitoneally, or intramuscularly.
Immune cells at mucosal surfaces include dendritic cells (DC), monocytes and macrophages, neutrophils, and B cells, and in some species such as cattle and other ruminants, a specialized subset of T cells known as gamma-delta T cells (γδ T cells). In addition, epithelial cells lining mucosal surfaces can also respond to immune stimuli. The coordinated activation of immune cells and epithelial cells can induce immune responses to suppress infection by either prevent viral or bacterial infection, or significantly reduce the severity of infection and limit pathogen replication. In addition, strong activation of local immune responses at mucosal surfaces can also reduce the severity of infection even after the infection has already been initiated (e.g., when the immune stimulus is administered in an early therapeutic setting as opposed to for prophylaxis).
When immune stimuli reach mucosal surfaces, they are sampled by local DC and macrophages, which then become activated and produce cytokines and chemokines, including inflammatory cytokines (e.g., TNF, IL-1, IL-6) as well as antiviral and antibacterial cytokines (e.g., IFN-γ, IFN-α, INF-β) and other cytokines such as IL-12 and IL-22. The epithelial cells also respond to immune stimuli and produce chemokines (and cytokines) that serve to recruit immune cells to the sites of inflammation. Key chemokines produced by epithelial cells include MCP-1, which recruits monocytes, and IL-8, which recruits neutrophils. Monocytes and neutrophils both play key role in early immune defenses against viral and bacterial pathogens of the respiratory tract and other mucosal surfaces. Some immune stimuli can also directly activate a specialized type of T cell (γδ T cell) that is only found at mucosal surfaces, especially in cattle and other ruminants, and also another cell type known as NK cells, which are present in all mammalian species.
The early cytokine and chemokine responses serve to amplify local immune responses and recruit other inflammatory cells, including monocytes, neutrophils, NK cells and later conventional T cells. These other inflammatory cells produce antiviral and antibacterial cytokines, and also secrete factors such as reactive oxygen and reactive nitrogen species that can directly kill certain bacteria and viruses. In addition, these immune cells and epithelial cells can also produce antimicrobial peptides that kill bacteria and enhance the activity of antibiotics.
To activate mucosal immune defenses effectively, an immune stimulant needs several important properties. These include the ability to first adhere well to epithelial surfaces, and in some cases penetrate into and around epithelial cells. In some embodiments disclosed herein, cationic liposomes are useful for delivering or transporting nucleic acid molecules such as polyIC and plasmid DNA or CpG oligonucleotides into cells such as epithelial cells and immune cells.
In some embodiments, it is understood that an effective mucosal immune stimulant also needs to be very potent, given the large surface areas that must be contacted by relatively small volumes of the immune stimulant. In addition, the ability to induce broad spectrum immune responses, by activating both antibacterial and antiviral immune pathways, is important. Thus, activation of the TLR3 pathway induces anti-viral immune responses, while activation of the TLR9 pathway induces antibacterial immune responses. By activating both pathways simultaneously, the breadth and potency of the immune response that is induced is greatly increased.
In immunostimulatory compositions disclosed herein an effective mucosal immune stimulant should be capable of interacting with epithelial cells and immune cells for prolonged periods of time in order to induce a sustained immune response. In accordance with embodiments disclosed herein, addition of a mucosal adhesion agent serves to disperse the immune stimulant over large mucus membrane surfaces, and also prolongs the contact time. For example, addition of a mucosal adhesion agent such as carboxymethylcellulose to an immune stimulant such as a liposome-TLR agonist complex can induce local immune stimulation at mucosal surfaces for prolonged periods, for example more than 7 to more than 14 days or longer. Prolonged exposure to these compositions can reduce the risk of onset of an infection, treat an infection or treat a tumor or chronic ulcer in the region of application, for example. In addition, this period of time is sufficient for reducing the chance of infection from most pathogens; for example, exposures in respiratory disease settings. In other embodiments, with respect to treating a subject with the immunostimulatory compositions disclosed herein of use as a mucosal immune stimulants, this prolonged duration of immune activation provides a more effective long-term therapeutic response. In certain embodiments, prolonged exposure to the immunostimulatory compositions disclosed herein can lead to generation of T cell responses and antibody responses (as described further below).
In other embodiments, an antigen (e.g. protein antigen against a pathogen or other agent) in combination with immunostimulatory compositions disclosed herein can be used as a vaccine. In accordance with these embodiments, all components of the adjuvant or vaccine are present in the same pharmaceutical composition, which may be a liquid composition and which may further comprise one or more excipients, diluents or carriers. The pharmaceutical compositions may be sterile. In certain embodiments, the liposome-TLR ligand complexes include a protein antigen.
In accordance with these embodiments, immunostimulatory compositions (e.g. MiM) in combination with a high viscosity and/or high molecular weight adhesion agent can form a viscous composition and be used as a pharmaceutical agent for delivering to the eye of a subject. In accordance with these embodiments, immunostimulatory compositions disclosed herein can further include one or more excipients, diluents or carriers. In other embodiments, immunostimulatory compositions disclosed herein can include higher or lower concentrations of the high viscosity and/or high molecular weight adhesion agent depending on the condition and how long exposure is desired in the targeted area (e.g. eye) and the condition being treated (e.g. chronic infection, tumor or injury). In certain embodiments, the consistency of the immunostimulatory composition having high viscosity and/or high molecular weight adhesion agents can be where the composition is capable of being delivered by a drop bottle but the composition remains in the delivery location for a prolonged period (e.g. on the cornea).
In certain embodiments, immunostimulatory compositions disclosed herein include an immunostimulant of cationic liposomes complexed to TLR agonists (plasmid DNA and/or polyinosinic polycytidylic acid; pIC), and low- or medium-molecular weight carboxymethylcellulose (CMC) as an adhesive agent to increase uptake and trafficking to lymph nodes. In some embodiments, immunostimulatory compositions disclosed herein include: 1) cationic liposomes (e.g., DOTAP and cholesterol, 1:1 molar ratio); 2) non-coding plasmid DNA and/or CpG oligonucleotides (e.g., 50 ug/ml); 3) synthetic pIC (e.g., 50 ug/ml, about 1.0 to about 10.0 kb); 4) carboxymethylcellulose (CMC) (e.g., 5%-15% v/v). In other embodiments, immunostimulatory compositions disclosed herein can include an immunostimulant of cationic liposomes complexed to TLR agonists (plasmid DNA, CpG oligonucleotides and/or polyinosinic polycytidylic acid; pIC), and low- and/or medium-molecular weight carboxymethylcellulose (CMC) as an adhesive agent; and/or high molecular weight/high viscosity carboxymethylcellulose. In some embodiments, immunostimulatory compositions disclosed herein include: 1) cationic liposomes (e.g., DOTAP and cholesterol, 1:1 molar ratio); 2) non-coding plasmid DNA and/or CpG oligonucleotides (e.g., 50 ug/ml); 3) synthetic pIC (e.g., 50 ug/ml, about 1.0 to about 10.0 kb); and 4) carboxymethylcellulose (CMC, low molecular weight of about 5-15.0% v/v) and optionally, 5) high viscosity/high molecular weight carboxymethylcellulose.
In one embodiment, to prepare the adjuvant, complexes of cationic liposomes with DNA and pIC are prepared, then the antigen is added. The final step is the addition of the CMC adhesive agent. In particular embodiments, the vaccine is then administered by the s.c. or i.m. route. In various embodiments, this vaccine technology is applicable to the treatment and prevention of both infectious disease and cancer vaccine applications.
In certain methods disclosed herein, to generate immunity with vaccine adjuvants and antigens, T cells can be stimulated. While T cells play little direct role in mucosal immune responses, they are important for longer term protection against viral, fungal, prion, protozoan and bacterial infections, as in the case of conventional prophylactic vaccines. Further, T cells also play an important role in cancer immunity.
In some embodiments, CD4+ and CD8+ T cells initiate and/or enhance cell mediated immunity and humoral immunity. CD8+ T cells interact with antigens displayed on MEW Class I molecules. CD4 T cells recognize antigenic peptides bound to MEW class II molecules. Upon interaction with a MHC class II molecule, the CD4 cells secrete factors such as cytokines, which activate B cells, cytotoxic T cells, macrophages, and other cells that participate in an immune response.
As referenced herein, certain compositions disclosed herein can be referred to as an “LTC” immunotherapeutic composition which includes at least the following agents, synthetic TLR9-stimulatory CpG oligonucleotides (or plasmid DNA or other TLR9 ligand) and synthetic TLR3 double stranded RNA molecules (e.g. poly(I/C) or other TLR3 ligand) complexed to cationic liposomes and carboxymethylcellulose. In certain embodiments, synthetic TLR9-stimulatory CpG oligonucleotide-containing formulation can be referred to as LTC 2.0. In accordance with these embodiments, this immunogenetic composition is designed to induce or enhance innate immune responses in multiple different mammalian species, following topical, local or parenteral administration. In certain embodiments, synthetic TLR9-stimulatory CpG oligonucleotides can be used interchangeably with plasmid DNA-containing formulations disclosed herein. In other embodiments, LTC or similar formulation containing synthetic TLR9-stimulatory CpG oligonucleotides can be used alone or in combination with other agents (e.g. a vaccine or treatment for an infection etc.) to enhance innate immunity in the subject and reduce onset, prevent or treat a condition. In certain embodiments, the condition includes a pathogenic organism infection (e.g. viral, bacterial or fungal infection). In some embodiments, these formulations can be used to prevent or treat an eye infection. In other embodiments, these formulations can be used to reduce or prevent viral shedding and reduce or prevent infectivity of viral agents to another host.
In some embodiments, a vaccine against an infection or other standard composition of use to treat an infection can be used in combination with immunostimulatory compositions disclosed herein such as LTC containing CpG oligonucleotides. In some embodiments, a vaccine against a viral infection or treatment against a viral infection can be administered before, during or after administering an immunostimulatory composition disclosed herein. In certain embodiments, antimicrobial agents along with an immunostimulatory composition disclosed herein can be used to induce specific and non-specific immune responses.
In some embodiments, other adjuvants may be present in the immunostimulatory compositions and vaccines of the present invention, or delivered in combinations, including those that stimulate either or both a TH1 and/or TH2 response. TH1 adjuvants suitable for use in the invention may include, for example, saponin formulations, virosomes and virus like particles, non-toxic derivatives of enterobacterial lipopolysaccharide (LPS), immunostimulatory oligonucleotides. Immunostimulatory oligonucleotides, such as oligonucleotides containing a CpG motif, are typical TH1 adjuvants. TH2 adjuvants suitable for use in the invention include, for example, mineral containing compositions, oil-emulsions, and ADP-ribosylating toxins and detoxified derivatives thereof. Mineral containing compositions, such as aluminum salts are typical TH2 adjuvants for use in the invention.
Other agents that can be included in the immunostimulatory compositions disclosed herein can include an agent or anti-microbial or adjuvant agent known or used in the art, including but not limited to: CLDC adjuvants, mineral salts, such as aluminum salts and calcium salts, hydroxides (e.g., oxyhydroxides), phosphates (e.g., hydroxyphosphates, orthophosphates) and sulfates, etc.; oil-in-water emulsions, such as squalene-water emulsions, including MF59 (5% Squalene, 0.5% Tween 80, and 0.5% Span 85, formulated into submicron particles using a microfluidizer); complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA); saponin formulations, such as QS21 and ISCOMs; virosomes and virus-like particles (VLPs); bacterial or microbial derivatives, such as non-toxic derivatives of enterobacterial lipopolysaccharide (LPS), Lipid A derivatives; immunostimulatory oligonucleotides, such as IC-31 (deoxynucleotide comprising 26-mer sequence 5′-(IC)13-3′ and polycationic polymer polypeptide comprising 11-mer amino acid sequence KLKLLLLLKLK) and ADP-ribosylating toxins and detoxified derivatives thereof; human immunomodulators, including cytokines, such as interleukins (e.g., IL-1, IL-2, IL-4, IL-5, IL-6, IL-7, IL-12, interferons (e.g., interferon-gamma), macrophage colony stimulating factor, and tumor necrosis factor; bioadhesives and mucoadhesives, such as chitosan and derivatives thereof, esterified hyaluronic acid microspheres or mucoadhesives, such as cross-linked derivatives of poly(acrylic acid), polyvinyl alcohol, polyvinyl pyrollidone, polysaccharides and carboxymethylcellulose; microparticles (e.g., a particle of about 100 nm to about 150 um in diameter) formed from materials that are biodegradable and non-toxic (e.g., a poly(alpha-hydroxy acid), a polyhydroxybutyric acid, a polyorthoester, a polyanhydride, a polycaprolactone, etc.); liposomes; polyoxyethylene ethers and polyoxyethylene esters; PCPP formulations; muramyl polypeptides, including N-acetyl-muramyl-L-threonyl-D-isoglutamine (thr-MDP), N-acetyl-normuramyl-l-alanyl-d-isoglutamine (nor-MDP), and N-acetylmuramyl-l-alanyl-d-isoglutaminyl-l-alanine-2-(1′-2′-dipalmitoyl-s-n-glycero-3-hydroxyphosphoryloxy)-ethylamine MTP-PE); and imidazoquinolone compounds, including Imiquamod and its homologues (e.g. “Resiquimod 3M”). Illustrative adjuvants suitable for use include, but are not limited, to cationic lipid DNA complexes (CLDC), CpG-oligonucleotides, poly I:C, LPS, alpha-galactosylceramide, and the like.
In certain embodiments, the immunostimulatory compositions of the present invention are combined with an antigen, or administered sequentially to a subject to induce an enhanced immune response. In some embodiments, the compositions disclosed herein can include a protein antigen or antigen derived from a pathogenic agent. In some embodiments, the antigen is a viral, fungal, protozoan, prion or bacterial antigen. In other embodiments, compositions (e.g., vaccines) and kits of the invention include an antigen, and certain methods of the invention comprise administering an antigen. In certain embodiments, the antigen present in the vaccine compositions provided by the invention can be any material or substance that can induce an immune response (e.g., cellular and/or humoral immune response) by the immune system of a human or animal. For example, the antigen can be a polypeptide of interest derived from an infectious agent, e.g., a bacterium, a virus, a fungus, a protozoan, a parasite, or a prion. The antigen can be a whole microbe or a mixture thereof. The compositions can include a live whole infectious agent. In certain embodiments, the compositions can include a killed or inactivated (attenuated) infectious agent such as a virus or other microbial agent.
In certain embodiments, the antigen includes, e.g., a polypeptide, nucleic acid, polysaccharide, a fatty acid or the like, derived from a pathogenic agent. In other embodiments, the antigen can be a subunit or fragment of a polypeptide, or a fragment of a nucleic acid or polysaccharide derived from a pathogenic agent. In certain embodiments, the antigen is a recombinant polypeptide produced in a heterologous expression system, e.g., a recombinant protein derived from an infectious agent that was expressed in and purified from cells of another organism. However, an antigen can also be a recombinant nucleic acid construct which encodes a polypeptide antigen of interest (e.g., an expression construct). The antigen can include a viral subunit, a virus-like particle, a capsular (poly) saccharide; a bacterial outer membrane bleb formation containing one or more of bacterial outer membrane proteins, a phospholipid, a lipopolysaccharide, or a polysaccharide.
In some embodiments, the antigen can be a naturally occurring substance. In certain embodiments, the antigen comprises or is derived from an allergen, e.g., pollen. In certain embodiments, the antigen comprises or is derived from a toxin. In certain embodiments, the antigen comprises or is derived from an addictive substance, including, without limitation, nicotine, caffeine, alcohol, and the like. In yet other embodiments, the antigen can be a non-naturally occurring (e.g., synthetic) substance, e.g., a synthetic peptide, a synthetic polysaccharide, or a synthetic polymer.
In other embodiments, the antigen is a tumor cell or is derived from a tumor cell, including cells from any of the types of cancers or tumors described herein.
In certain aspects, the antigen can be provided in a vaccine, e.g., any vaccine known in the art. The vaccine can be a nucleic acid construct (e.g., a DNA vaccine). The vaccine can be a viral vector vaccine, which uses live viruses to carry DNA into an individual's cells. The DNA contained in the viral vaccine encodes antigen(s) that, once expressed in the infected cells, elicit an immune response. Alternatively, the vaccine can be a subunit vaccine, e.g., a specific protein from a virus. The vaccine can be a dendritic cell vaccine, in which an individual's dendritic cells are cultured with an antigen and then re-injected into the individual to stimulate an immune response. In certain embodiments, the vaccine can be a monovalent vaccine, e.g., containing a single antigen. In certain embodiments, the vaccine containing the antigen is a polyvalent or multivalent vaccine, e.g., containing more than one antigen.
The amount of antigen to be included in compositions disclosed herein and used in the methods of the present invention depends on the target and on immunogenicity of the antigen itself and the efficacy of any adjuvants co-administered therewith. In general, an immunologically effective dose can include but is not limited to a concentration of about 1 μg to about 1000 μg of the antigen, about 5 μg to about 500 μg, m about 10 μg to about 200 μg. In some embodiments, an immunologically effective dose can be at least about 1 μg, at least about 5 μg, at least about 10 μg, at least about 25 μg, at least about 50 μg, at least about 100 μg, at least about 150 μg, at least about 200 μg, at least about 250 μg, at least about 300 μg, at least about 350 μg, at least about 400 μg, at least about 450 μg, at least about 500 μg, at least about 550 μg, at least about 600 μg, at least about 650 μg, at least about 700 μg, at least about 750 μg, at least about 800 μg, at least about 850 μg, at least about 950 μg, or up to about 1000 μg of antigen. In embodiments where the antigen is a recombinant protein or peptide, a suitable dose can be about 10-100 μg. In embodiments where the antigen is a recombinant protein or peptide, a suitable dose can be about 10-100 μg.
In some embodiments, the present invention can include pharmaceutical compositions designed for mucosal immune stimulation as well as other non-specific immune stimulation. In accordance with these embodiments, the composition includes a liquid immune stimulant, formulated with a pharmaceutically acceptable carrier, diluent or excipient. In other embodiments, the composition includes a viscous solution having HMW/high viscosity adhesion agents with improved adhesion properties formulated with a pharmaceutically acceptable carrier, diluent or excipient. Any known diluents, excipients and carriers in the art are contemplated of use herein. Compositions may be in an aqueous form or semi-solid form capable of being delivered through a dropper. In the most desirable formulation, the immune stimulant would be prepared as a stable liquid (during refrigeration) in an acceptable carrier. In other instances, the immune stimulants may be lyophilized during manufacture, to be reconstituted later into an aqueous form at the time of use. In certain embodiments, composition of the instant invention can be liquid, semi-liquid, semi-solid or dried, such as a lyophilized formulation. In certain embodiments, the compositions can be a stable liquid, semi-liquid, or semi-solid formulation stable at room temperature for prolonged periods.
In some embodiments, immunostimulatory compositions disclosed herein are very stable having improved tolerance for high temperatures or moderate temperatures for prolonged periods. In accordance with these embodiments, the immunostimulatory compositions with or without high viscosity/HMW adhesion agents are stable at room temperature (e.g. 25° C.) for at least one week, at least one month, at least 2 months, at least 3 months, at least 4 months or more. For example, these compositions are stable during autoclaving, an advantage for assuring sterility of the compositions during the manufacturing process and for use in a subject having a health condition contemplated herein.
In certain embodiments, pharmaceutical compositions of the present invention are formulated for delivery by a variety of mucosal routes of delivery, including intranasally, orally, intrarectally, intravaginally, or by the intra-mammary or intra-uterine route, or by aerosol mist exposure, or by dilution in water (fish). Alternative routes of delivery include parenterally, e.g., intravenously, subcutaneously, intraperitoneally, or intramuscularly.
In some embodiment, compositions disclosed herein can be included in one or more containers or vials, e.g., single use or multiuse containers or vials. In some embodiments, multiuse vials can include a rubber diaphragm suitable for retrieving multiple doses of the immune stimulant. The composition may also be supplied in flexible plastic bags that can be connected to multi-dose intranasal syringes, as in a feedlot operation. The composition may also be further diluted in a suitable diluent for administration in an aerosol delivery device that can be worn as a backpack for administration to poultry, or in a dispensing device suitable for delivery into water for treatment of fish. In some embodiment, immunostimulatory compositions disclosed herein can be delivered using any delivery device depending on viscosity of the formulation and desired mode of administration and the condition to be treated or prevented. In certain embodiments, immunostimulatory formulations or compositions disclosed herein can be delivered by a dropper bottle, a tube, an eye delivery device, an atomizer, an inhaler, a syringe or other suitable container. In other embodiments, the immunostimulatory composition can be part of a kit and further include a delivery device. In certain embodiments, compositions included in kits disclosed herein can include LTC or other synthetic TLR9-stimulatory CpG oligonucleotide-containing compositions.
In some embodiments, the kit or composition can include for a single dose, or multiple doses. In some embodiments, a kit containing an immunostimulatory composition can include a preservative. In some embodiments, a delivery device can include a bulb tip or other delivery tip. In other embodiments, a syringe can be used to or is adapted for use to deliver the composition to by any delivery mode contemplated herein. For example, delivery directly to the nasal cavity, oral cavity, to the affected region, to the eye (e.g. cornea) and/or pharyngeal region of a mammal. In certain embodiments, the subject is an animal such as a mammal (e.g. horse, dog, cat, cow, pig, sheep, goat, rabbit) or bird (e.g. chicken, turkey, duck) or fish (e.g., tilapia, salmon, trout, catfish).
In certain embodiments, methods of inducing an immune response in a subject are disclosed. In certain embodiments, immunostimulatory compositions disclosed herein are administered to a subject in order to induce a non-specific immune response. In certain aspects, the composition is administered in a therapeutically effective amount. In further aspects, the composition is administered in a prophylactically effective amount. In yet other embodiments, doses for treatment of cattle can be in the range of 1 ml to 5 ml of immunostimulatory compositions disclosed herein (e.g. PCT-01) administered into each nostril, for goats and sheep, 0.5 ml to 3 ml immunostimulatory compositions disclosed herein (e.g. PCT-01) in each nostril, for dogs 0.1 ml to 3 ml immunostimulatory compositions disclosed herein (e.g. PCT-01) in each nostril (and 1 ml to 5 ml orally), for cats 0.1 ml to 2 ml immunostimulatory compositions disclosed herein (e.g. PCT-01) in each nostril and 0.5 to 3 ml orally. For treatment of poultry, an example dose could be 1 to 100 ml immunostimulatory compositions disclosed herein (e.g. PCT-01) diluted in 100 to 1000 ml of suitable diluent (e.g., saline, D5W) and administered as an aerosol to treat a 30 by 30 foot room with 100 chickens. For treatment of fish, and example dose can be about 1.0 to 50 ml immunostimulatory compositions disclosed herein (e.g. PCT-01) diluted in 1000 to 10,000 gallons of water for 24 h of treatment. In humans, the intranasal dose of immunostimulatory compositions disclosed herein (e.g. PCT-01) can be about 0.1 to about 2 ml administered in each nostril.
In some embodiments, the subject is a mammal at risk of infection by a pathogenic agent (or already infected with such an agent), such as a virus, fungus, prion, protozoan or bacterium, or an infected subject. Examples include but are not limited to: 1) prevention or early treatment of kennel cough in dogs, or upper respiratory tract infection syndrome in cats; 2) prevention or early treatment of bovine respiratory tract disease (BRD) syndrome in cattle (beef or dairy); 3) prevention or early treatment of respiratory tract disease in swine, sheep, or goats; 4) intra-mammary infusion for prevention or treatment of mastitis in cattle; 5) intra-uterine infusion for prevention or early treatment of metritis in cattle or horses; 6) oral administration for treatment of inflammatory bowel disease in dogs, cats, humans; and 7) intra-nasal administration for prevention or early treatment of viral upper airway infections in humans.
In another embodiment, the subject is a bird at risk of infection, or already infected. Examples of methods for birds include, but are not limited to, prevention or treatment of viral, fungal, protozoan, or bacterial respiratory tract infections (e.g., influenza infection) in poultry (e.g. chickens, turkeys, ducks) in intensive rearing conditions (e.g., boiler operations, egg laying facilities). In addition, the composition could be directly administered to eggs (e.g. in ovo) for induction of innate immune responses in the developing embryo to improve hatchability and early resistance to infection.
In another embodiment, methods are disclosed to treat fish for example to treat an infection or reduce onset of an infection in a fish population. Examples include but are not limited to prevention or treatment of viral, fungal, bacterial or protozoal infections in fish. For example, in fish farms. Examples include, but are not limited to, fish in aquaculture settings (e.g., tilapia, trout, salmon, catfish), where an immunostimulatory composition could be administered by diluting in water in small treatment ponds or tanks for periods of several hours of days of treatment.
In certain embodiments, the composition is provided by a variety of mucosal routes of delivery, including intranasally, orally, inter-rectally, intravaginally, or by the intra-mammary or intra-uterine route, or by aerosol mist exposure, or by dilution in water (e.g., fish). Alternative routes of delivery include parenterally, e.g., intravenously, subcutaneously, intraperitoneally, or intramuscularly.
According to certain embodiments, administration of the composition is applied to a mucosal surface. According to certain exemplary embodiments, the composition is applied topically to the nose, eyes, mouth, upper airways, air sacs, gills, ears, eyes, uterus, mammary gland, and or gastrointestinal tract.
According to certain alternative embodiments, methods disclosed herein concern inducing an immune response, e.g., an immune response specific to an antigen, by providing a composition (e.g., a vaccine composition) of the present invention to a subject in need thereof. In particular embodiments, the subject is a mammal at risk of an infection due to a pathogen.
Particular embodiments include methods of treating or preventing an infection, for example, a lung infection. In accordance with these embodiments, immunostimulatory compositions disclosed herein can be used to treat or reduce the onset of an infection by administering to a subject in need thereof an effective amount of the immunostimulatory composition in combination with an antigen, e.g. a protein antigen, an antigen derived from a virus, fungus, prion, or bacterium. In other embodiments, an immunostimulatory compositions disclosed herein can be used to reduce onset of an infection such as a lung infection or other infection caused by a pathogen. In accordance with these embodiments, the immunostimulatory composition can be administered to the subject prior to traveling, when exposed to a pathogenic agent or suspected exposure, to reduce onset of the infection in the subject.
In some embodiments, include treating or preventing a cancer in a subject in need thereof, including providing to the subject an effective amount of a cancer antigen in combination with an immunostimulatory composition of the present invention. In other embodiments, immunostimulatory compositions disclosed herein can be used to treat a subject having cancer for inhibiting tumor growth, reducing tumor size, and inhibiting tumor metastasis, as well as reducing side effects of tumors such as chronic ulcers. In some embodiments, tumor growth, tumor size, or tumor metastasis is inhibited or reduced by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, or 90% in a subject when compared to treatment without immunostimulatory composition as disclosed herein. In certain embodiments, the subject has a tumor (e.g. a metastatic tumor). In other embodiments, the subject is considered to be at risk of cancer or tumor metastasis.
In some embodiments, a tumor can be any type of tumor from any type of cancer such as a solid tumor or liquid tumors or other tumor. In certain embodiments, the cancer is breast cancer, lung cancer, prostate cancer, colorectal cancer (e.g., colon carcinoma), brain cancer, glioblastoma, skin cancer, melanoma, eye, cancer esophageal cancer, stomach cancer, bladder cancer, pancreatic cancer, cervical cancer, head and neck cancer, ovarian cancer, melanoma, leukemia, myeloma, lymphoma, glioma, Non-Hodgkin's lymphoma, leukemia, multiple myeloma or multidrug resistant cancer.
Some embodiments disclosed herein can include combination therapies using immunostimulatory compositions disclosed herein in combination with immunotherapy. Immunotherapies can include use of cytokines, monoclonal antibodies, and/or vaccines. In certain embodiments, targeted therapy can include chemotherapy drugs.
In some embodiments, an effective amount of an immunostimulatory composition can include about 0.1 ml to about 5.0 ml (e.g., about 1 ml) of TLR ligand; and about 1% to about 20%, about 2% to about 15%, about 2.5% to about 10%, about 5% to about 10%, or about 5% (v/v) of a cellular adhesion agent, such as carboxymethylcellulose or a PEG. In some embodiments, the effective amount includes: optionally, 100 to 500 ug of antigen; and about 1-4 ml of cationic liposome-DNA complexes; about 5% to about 10% (v/v) of carboxymethylcellulose.
In certain embodiments, the immunostimulatory compositions alone or in combination with an antigen can be administered in a single dose or in two, three, four, five, six, seven, eight, nine, ten or more dosing regimens. In some embodiments, the immunostimulatory composition can be provided daily, every other day, twice a week, weekly, every other week, once a month, or once every other month depending on the condition (e.g. eye condition)
The following examples are included to illustrate various embodiments. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered to function well in the practice of the claimed methods, compositions and apparatus. However, those of skill in the art should, in light of the present disclosure, appreciate that changes may be made in the some embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
In one exemplary method, to test the effects of adding carboxy-methylcellulose (CMC) on the adhesion properties of liposome-TLR3/9 complexes, complexes of liposomes and DNA and TLR3/9 agonists (plasmid DNA and pIC) were labeled with a fluorescent dye, and adherence to a rat epithelial cell line was evaluated by a 3 h assay with shaking in an incubator. The effects of adding different concentrations of CMC to liposome-TLR3/9 complexes (CALNAC) was assessed by flow cytometric measurement of the percentages of epithelial cells containing liposome-TLR3/9 complexes. As best shown in
In one exemplary method, in order to assess the effects of combining TLR3 and TLR9 agonists with liposomes, spleen cells from mice were incubated with cationic liposomes alone, or liposomes+pIC or liposomes plus pDNA, or liposomes plus both pIC and pDNA. Immune stimulation (IL-12 release) was measured by ELISA assay. The combination of both TLR3 and TLR9 agonists generated synergistic immune activation. See for example,
In another exemplary method, in order to evaluate the effect of CMC on the immune potency, canine PBMC were incubated with CLDC complexes or CLDC+10% CMC for 24 h. IFN-γ release measured by ELISA as an indication of immune stimulation potency.
In another exemplary method, to test the ability of CMC to affect adhesion to mucosal surfaces, mice were administered intranasally 50 μl CLDC or CLDC+CMC that had been labeled with a fluorescent dye to allow tracking in a live animal imager (IVIS). The amount of labeled material still present in the nostrils 60 min after administration was determined by live animal imaging. As illustrated in
In yet another exemplary method, to assess the effect of CMC on the ability of CLDC to elicit immune response, mice (n=3 per group) were administered CLDC or CLDC+CMC (ie, PCT-01) orally, and 24 hours later, infiltrates of immune cells into the oropharynx was assessed by flow cytometry, using cells obtained from the oropharynx by swabs. As illustrated in
In one exemplary method, to assess the effect of CMC on the ability of CLDC to elicit immune response in felines, cats were treated intranasally with PCT-01 (CLDC+CMC) (n=5) and compared to cats treated with CLDC (n=4). To conduct the study, liposomes were labeled with a fluorescent dye to track their uptake by cells in the nasal and oropharyngeal mucosal. Healthy purpose-bred cats were treated intranasally with 0.3 ml labeled CLDC+CMC or labeled CLDC in each nostril. 24 hours later, nasal lavage samples were obtained and the percentage of cells that had contained labeled liposomes (TopFluor+) were compared between treatment groups, using flow cytometry. As illustrated in
In another exemplary method, to test the ability of PCT-01 to affect clinical signs of ocular disease, a challenge study with feline herpesvirus type 1 (FHV-1) was conducted in purpose-bred cats. Three groups of cats (n=7 per group) including untreated control cats (group 156), cats pre-treated with PCT-01 24 h prior to challenge (group 121) and cats treated with PCT-01 when symptoms first developed (group 144), were monitored for clinical signs of infection (ocular signs, total clinical signs, body temp) and viral shedding by qRT-PCR for 28 days after the viral challenge was administered. As illustrated in
In another exemplary method, to assess the uptake of labeled PCT-01 by nasal and oropharyngeal cells in dogs, labeled PCT-01 were administered intranasally and orally to a healthy adult dog. 6 h and 20 h later, nasal lavage and throat swab samples were obtained, and the percent of cells containing labeled liposomes determined. As illustrated in
In another exemplary method, to assess the stimulation of immune cell infiltrates into nose and throat of dogs, PCT-01 was administered intra-nasally (0.5 ml per nostril) and orally (2 ml) in a healthy adult dog. The effects on immune cell infiltrates in the nose and throat was determined 6 h and 20 h later. As illustrated in
In another study, expression of cytokine genes in the oropharynx of dogs treated with PCT-01 was assessed at 3 time points (24 h, 72 h, 7 days) following treatment in healthy Beagle dogs (n=5), using qRT-PCR and primers designed for amplification of canine cytokine genes. As illustrated in
In some exemplary methods, to assess the ability of an exemplary formulation disclosed herein (e.g. PCT-01) to elicit a bovine immune response, cattle (n=5 per group) were treated by intranasal administration of 3 different doses of PCT-01 (2 ml, 4 ml, or 6 ml per animal, divided in two equal doses per nostril) using a nasal cannula. One additional untreated group served as a control. Prior to the initial dose, and then at 24 hours, 72 hours, 1 week and 2 weeks post administration, swabs of the throat were obtained from each animal, and the cells were removed from the swab by swirling and total cell counts obtained. As illustrated in
In some exemplary methods, to assess the ability of an exemplary formulation disclosed herein (e.g. PCT-01), monocyte recruitment and immune activation were analyzed after administration to the oropharynx of cows. Cattle (n=5 per group) were treated with intranasal administration PCT-01 (e.g. MiM, about 4 ml) (2 ml per nostril) (or treated with saline only as a negative control, no Tx) and infiltrates of monocytes (CD14+ cells) in the nasopharynx were assessed by throat swabs and flow cytometric analysis. In addition, the upregulation of MHCII expression (measure of immune activation) was also assessed on the CD14+ monocytes by flow cytometry. As illustrated in
In some exemplary methods, to assess the ability of an exemplary formulation disclosed herein (e.g. PCT-01), to test the ability of PCT-01 to stimulate bovine cytokine production as markers of an enhanced immune response, cattle (n=5 per group) were administered PCT-01 intranasally (e.g. 2 ml or 4 ml) and cells obtained by nasopharyngeal swabbing were evaluated using qRT-PCR for cytokine expression. Several cytokine markers were evaluated for enhanced expression.
In some exemplary methods, to assess the ability of an exemplary formulation disclosed herein (e.g. PCT-01), to evaluate the ability of PCT-01 to induce an enhanced immune response relative to other immune stimulants known in the art, two groups of cattle (n=5) were administered either PCT-01 (e.g. 4 ml/2 mL per nostril) or Zelnate™ (I.M. per manufacturer guidance) and the immune response was measured; for example, prior to treatment, 24 hours post-treatment and 72 hours post-treatment.
In another exemplary method, in order to assess the immunological impact of an exemplary formulation disclosed herein (e.g. PCT-01), dairy cattle (n=5) were administered by infusion in one quarter of the mammary gland using PCT-01 (1 ml diluted in PBS). Pre-treatment lavage samples were obtained from the treated animal quarter 7d before infusion (pre-Rx) and then at 24 h, 72 h, and 7 days after PCT-01 infusion. As illustrated in
In another exemplary method, in order to assess the immunological impact of an exemplary formulation disclosed herein (e.g. PCT-01), goats were administered PCT-01. Nasopharyngeal swabs were obtained from healthy adult goats (n=6) before PCT-01 administration and at 24 h, 72 h, and 7 days after treatment. As illustrated in
In another exemplary method, in order to assess the immunological impact of an exemplary formulation disclosed herein (e.g. PCT-01) on monocyte response and cellular activation, monocyte infiltration and MHCII upregulation were assessed post administration. As illustrated in
As illustrated in
In some exemplary methods, a starting material is referred to as previously disclosed immunostimulatory composition, MucosImmune (MiM), and further includes a high viscosity carboxymethylcellulose (CMC). In these examples, MiM is mixed 50/50 v/v with 1% solution of high viscosity CMC to create a product (referred to as Ocummune). Addition of high viscosity CMC increases viscosity of Ocummune to gel-like consistency (typically, MiM is essentially a liquid). By adding the high viscosity agent, contact time is increased with the cornea and can assist with reducing corneal pain sensitivity. In other exemplary methods, a starting material is MucosImmune (MiM) and high molecular weight/high viscosity carboxymethylcellulose (CMC) MW=700 KDa of high viscosity (Sigma). In other exemplary methods, MiM can be mixed 50/50 v/v with 2% solution of high viscosity CMC to create final product (Ocummune) with final high viscosity CMC concentration of 1%. In some exemplary methods, Ocummune has a similar consistency to that of Surgilube™ (e.g. a common lube for placing urinary or nasopharyngeal catheters).
In one exemplary method, in order to assess the immunological impact of an exemplary immunostimulatory formulation disclosed herein on chronic ocular infection in a subject, experimental protocols were applied to an animal model (e.g. cats) having chronic ocular herpes virus infection. A cat was observed pre-treatment having a chronic ocular herpesvirus infection and then treated with MiM further including a high viscosity carboxymethylcellulose additive (e.g. Sigma about 500 to 1,000 kDa such as 700 kDa carboxymethylcellulose, salt, high viscosity CMC) termed Ocummune. In one exemplary experiment, safety of the novel formulation was tested on mice and demonstrated no adverse effects following topical application of the immunostimulatory composition. Within 4 days of treatment, the cat demonstrated significantly improved infection (
In another exemplary method, in order to assess the immunological impact of an exemplary immunostimulatory formulation disclosed herein on an eye-related cancer in a subject, experimental protocols were applied to an animal model (e.g. horses) having corneal cancer. Horses having corneal cancer were observed pre-treatment having eye tumors and then post treatment: at 2 weeks, 4 weeks and 6 weeks with MiM further including a high viscosity carboxymethylcellulose additive (e.g. Sigma about 500 to 1,000 kDa such as 700 kDa carboxymethylcellulose, salt) termed Ocummune. Within 2 weeks of treatment, positive responses were observed in 5 of 7 treated horses. No adverse effects observed. At 4 weeks, positive responses were observed where essentially no tumor remained in the cornea of 5 of 7 horses. At 6 weeks, positive results were continued to be observed. (See for example,
In another exemplary method, in order to assess the immunological impact of an exemplary immunostimulatory formulation disclosed herein on corneo-limbal squamous cell carcinoma (SCC) in a subject, experimental protocols were applied to an animal model (e.g. horses) having corneo-limbal SCC. Horses having this cancer were observed pre-treatment having eye tumors and then post treatment: at 2 weeks, 4 weeks and 6 weeks with MiM further including a high viscosity carboxymethylcellulose additive (e.g. Sigma about 500 to 1,000 kDa such as 700 kDa carboxymethylcellulose, salt) termed Ocummune. The horses (2 horses) were treated by topical application only. Within 2 weeks of treatment, positive responses were observed in both treated horses. No adverse effects observed. At 2-4 weeks, positive responses were observed where essentially no tumor remained in the cornea of 5 of 7 horses. At 6 weeks, positive results were continued to be observed. (See for example,
In other embodiments, certain compositions disclosed herein can be referred to as “LTC” immune therapeutic which includes at least the following agents, synthetic TLR9-stimulatory CpG oligonucleotides and synthetic TLR3 double stranded RNA molecules complexed to cationic liposomes and carboxymethylcellulose. This immune therapeutic is designed to activate innate immune responses in multiple different mammalian species, following topical or parenteral administration. Screening studies with cell lines or leukocytes demonstrate innate immune activating properties across multiple species, and in multiple different cell types.
In another exemplary method, an LTC formulation was tested for activation of innate immune responses in a species exposed to the composition. In order to assess activation of innate immune responses in canine macrophages following incubation with the LTC immunotherapeutic. A canine macrophage cell line (MH588) was incubated with 3 different concentrations of the LTC immune stimulant. Twenty-four hours later, conditioned medium was collected, and secretion of TNF-α was measured by commercial ELISA. The immune stimulant was found to stimulate a dose-dependent increase in production of TNF-α, indicative of innate immune stimulation activity. See for example,
In another exemplary method, an LTC formulation was tested for activation of innate immune responses in cattle cells exposed to the composition. In this exemplary method, leukocytes from healthy cattle were placed in culture and stimulated with increasing concentrations of LTC, and supernatants were collected 24 h later, and assayed for IFN-γ release by ELISA. A dose-dependent increase in IFN-γ production was noted, consistent with LTC-induced innate immune activation. See for example,
In another exemplary method, formulations were compared for immunostimulation potency in an adult animal cell model. In this method, superior immune activation was observed in adult cattle using an improved immunostimulatory composition compared to untreated and a control composition. These immunostimulatory compositions include a formulation of complexes prepared with CpG oligonucleotides (LTC 2.0) compared to a formulation (LTC 1.0) prepared with non-coding plasmid DNA. The relative immune stimulatory potency of the new LTC formulation (LTC 2.0) was compared to that of the other formulation (LTC 1.0) using blood leukocytes prepared from healthy adult cattle. Leukocytes were stimulated for 24 h with 50 μl/ml of LTC 1.0 or LTC 2.0, then supernatants collected and secreted IFN-γ measured by ELISA. The LTC 2.0 immune stimulant was significantly more immunostimulatory than the LTC 1.0 immune stimulant at activating innate immunity, reflected by IFN-γ production. Because LTC 1.0 and LTC 2.0 compounds differ only in the TLR9 ligand (the TLR3 ligand polyI:C remains the same in both formulations), this observation supports that the combination of CpG oligos and polyIC is more potent than non-coding plasmid DNA and poly IC. See for example
In another exemplary method, formulations were compared for immunostimulation potency in an adult animal cell model. In this method, superior immune activation was observed in calves using an improved immunostimulatory composition compared to untreated and a control composition. These immunostimulatory compositions include a formulation of complexes prepared with CpG oligonucleotides (LTC 2.0) compared to a formulation (LTC 1.0) prepared with non-coding plasmid DNA. The relative immunostimulatory potency of the new LTC formulation (LTC 2.0) was compared to that of the original formulation (LTC 1.0) using blood leukocytes prepared from healthy young (<6 weeks of age) dairy calves. Leukocytes were stimulated for 24 hr with 50 μl/ml of LTC 1.0 or LTC 2.0, then supernatants collected and secreted IFN-γ measured by ELISA. The LTC 2.0 immune stimulant was significantly more active than the LTC 1.0 immune stimulant at activating innate immunity, reflected by IFN-γ production. Since LTC 1.0 and LTC 2.0 compounds differ only in the TLR9 ligand (the TLR3 ligand poly(I:C) remains the same in both formulations), this observation supports that the combination of CpG oligos and poly(I:C) is more potent than non-coding plasmid DNA and poly IC. These findings in calves are particularly noteworthy because it is often very difficult to stimulate strong immune responses in very young animals. Young mammals including humans are often those who are more prone to respiratory and GI infections. These health conditions are highly prevalent in cattle. See for example,
In another exemplary method, an LTC formulation was tested for activation of innate immune responses in swine cells exposed to the composition. In this exemplary method, a porcine macrophage cell line (3D4/21) was stimulated in vitro with increasing concentrations of the LTC formulation, and 24 hr later, supernatants were collected and assayed for release of IFN-α by ELISA. A dose-dependent increase in IFN-α production was noted, consistent with activation of innate immunity by LTC. It is also noted that LPS (a well-known TLR4 immune stimulant) did not induce IFN-α production, consistent with the unique innate immune activating properties of the LTC immune therapeutic. See for example,
In yet another exemplary method, an LTC formulation was tested for activation of innate immune responses in poultry cells exposed to the composition. In this exemplary method, a chicken macrophage cell line (HD11) was stimulated in vitro with increasing concentrations of LTC, and 24 h later, supernatants were collected and assayed for release of IFN-α by ELISA. A dose-dependent increase in IFN-α production was noted, consistent with activation of innate immunity by LTC. Further, LPS (a well-known TLR4 immune stimulant) did not induce IFN-α production in this exemplary method, consistent with the unique innate immune activating properties of the LTC immune therapeutic. See for example
In certain exemplary methods an LTC formulation includes, but is not limited to, cationic liposomes including DOTAP and cholesterol; CpG oligonucleotide (ODN D-SL03; 5′-tcg cga acg ttc gcc gcg ttc gaa cgc gg-3′ SEQ ID NO: 3 or 4 or similar); all bases phosphorylated; poly-inosinic, poly-cytidylic acid (e.g. molecular weight; avg. size 1.5 to 8 kb); and carboxymethylcellulose (about 10% v/v, low molecular weight).
In another exemplary method, an animal model was used to assess activity of an LTC formulation against infection in the eye of felines. In this method, reduction of clinical signs following treatment with LTC 2.0 (having TLR9 ligand, CpG ODN, and poly I:C) was observed. Cats with experimentally induced ocular infections with the feline herpesvirus FHV-1 were treated at the time they developed clinical signs (e.g. conjunctivitis, squinting, sneezing) twice daily with LTC 2.0 or placebo (e.g. artificial tears not containing an LTC formulation) for 10 days, and total clinical scores were measured. Treatment with LTC-O 2.0 significantly improved clinical symptoms of the infection in eyes of the treated felines compared to placebo. See for example
In yet another exemplary method, another animal model was used to assess activity of an LTC formulation against viral shedding to reduce transmission of the virus found in felines and other mammals. In treated versus control treated animals (placebo), a significant reduction of ocular viral shedding following treatment with an immunogenic formula (e.g. LTC 2.0 (having TLR9 ligand, CpG ODN, and TLR3 ligand, poly I:C)). In this example, cats with experimentally induced ocular infections (e.g. feline herpesvirus FHV-1) were treated in this example twice daily with LTC 2.0 or placebo (e.g. artificial tears) for 10 days, and virus shedding (measured by qRT-PCR) using daily eye swabs was measured. In this example, treatment with the experimental formulation, LTC-O 2.0, resulted in significantly reduced viral shedding compared to the placebo as measured by days of positive virus detection and reflected as percent positive. See for example
All of the COMPOSITIONS and METHODS disclosed and claimed herein may be made and executed without undue experimentation in light of the present disclosure. While the COMPOSITIONS and METHODS have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variation may be applied to the COMPOSITIONS and METHODS and in the steps or in the sequence of steps of the METHODS described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.
This application is a Continuation-in-Part of U.S. patent application Ser. No. 17/733,640, filed Apr. 29, 2022, that is a Continuation of PCT Application No. PCT/US20/58359, filed Oct. 30, 2020, that claims priority to continuation-in-part application, U.S. patent application Ser. No. 16/670,785 filed Oct. 31, 2019, now allowed, which is a continuation of U.S. patent application Ser. No. 15/476,723, filed Mar. 31, 2017, now U.S. Pat. No. 10,512,687, issued on Dec. 24, 2019 which claims priority to U.S. Provisional Application 62/456,505, filed Feb. 8, 2017, U.S. Provisional Application 62/316,986, filed Apr. 1, 2016, and U.S. Provisional Application 62/316,985, filed Apr. 1, 2016. This Continuation-in-Part application also claims priority to U.S. Provisional Application 63/223,815, filed Jul. 20, 2021. These applications are hereby incorporated by reference in their entirety for all purposes.
| Number | Date | Country | |
|---|---|---|---|
| 16670785 | Oct 2019 | US | |
| 62456505 | Feb 2017 | US | |
| 62316985 | Apr 2016 | US | |
| 62316986 | Apr 2016 | US | |
| 63223815 | Jul 2021 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/US2020/058359 | Oct 2020 | US |
| Child | 17733640 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 17733640 | Apr 2022 | US |
| Child | 17862475 | US | |
| Parent | 15476723 | Mar 2017 | US |
| Child | PCT/US2020/058359 | US |
