Patent Grant
10337032
The sequence listing contained in the electronic file titled “P32872US2_sequence_listing_ST25.txt,” created 31 Jan. 2017, comprising 1 KB, is hereby incorporated herein.
The present invention relates to recombinant (genetically engineered) microorganisms and methods of producing said microorganisms. Also provided are methods for producing biofuels by culturing the recombinant microorganisms.
All publications herein are incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. The following description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
Propane, a major component of autogas or liquefied petroleum gas (LPG), is an emerging fuel for future energy supply and transportation. Propane is the third most widely used motor fuel and about 20 million tonnes of propane gas are used per year to fuel motor vehicles. It is estimated that propane provides heat and energy for more than fourteen million homes worldwide annually. Propane also has an existing global market for a wide number of other stationary and mobile applications, such as low emission vehicles, gas burners and refrigeration systems. Easy separation from liquid biotechnological processes as a gas and less energy requirements for liquefaction and storage, offers potential advantages to propane over other gaseous fuels.
Natural metabolic pathways for the renewable biosynthesis of propane do not exist. The discovery of an aldehyde deformylating oxygenase (ADO) from cyanobacteria, however, has paved the way for synthetic alkane pathways to be constructed [Schirmer A et al. Science 2010, 329:559-562; Akhtar M K et al. Proc Natl Acad Sci USA 2013, 110:87-92; Howard T P et al. Proc Natl Acad Sci USA 2013, 110:7636-7641]. A microbial platform for propane generation dependent on fatty acid biosynthesis was recently reported [Kallio P et al., Nature communications 2014, 5:4731]. Kallio P et al. concluded that the pathway was limited by total flux through fatty acid synthesis (FAS). The most obvious example of this limitation comes from the markedly enhanced rate of propane synthesis observed when fatty acids were supplied to the external media. Herein, the inventors sought to bypass this limitation by generating new synthetic pathways that are not dependent on FAS. The inventors designed a series of modified butyraldehyde pathways based on the CoA-dependent butanol pathways commonly found in Clostridium spp. Propane biosynthesis was thereafter achieved by interrupting the route to alcohol by the addition of ADO (
The butanol pathway in Clostridium proceeds either via a keto acid route (Ehrlich pathway) or a CoA-dependent route. Higher yields of branched chain alcohols and aldehyde precursors (e.g. isobutyraldehyde) from the decarboxylation of keto acids make the Ehrlich pathway less attractive because ADO has a strong preference for straight chain aldehyde substrates. By contrast, butanol production by the CoA-dependent route initiates with the condensation of two molecules of acetyl CoA. Reduction in subsequent steps produces the end-product 1-butanol, via a butyraldehyde intermediate. There are several reports of engineered CoA-dependent butanol pathways in E. coli and other host organisms [Atsumi S et al. Nature biotechnology 2009, 27:1177-1180; Bond-Watts B B et al. Nat Chem Biol 2011, 7:222-227; Dellomonaco C et al. Nature 2011, 476:355-U131; Lan E I et al. J C Metab Eng 2011, 13:353-363; Lan E I. Proc Natl Acad Sci USA 2012, 109:6018-6023; Lan E I et al. Energ Environ Sci 2013, 6:2672-2681; Pasztor A et al. Biotechnology and bioengineering 2014]. Herein, the inventors constructed and evaluated a series of CoA-dependent butyraldehyde pathways that eliminate the dependency on AdhE2, thereby allowing butyraldehyde to be re-routed towards propane instead of butanol.
The use of ADO (from Prochlorococcus marinus MIT9313) as a terminal decarbonylase has been used for the production of medium/long chain (C9-C17) as well as short chain-length alkanes (C3, C7). Variant forms of ADO have demonstrated improved activity with the shorter chain aldehydes that are not encountered in native cyanobacteria. These variant forms of ADO are therefore attractive enzyme components for building new synthetic pathways with a greater productivity, as addressed herein.
Propane (C3H8) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel, in addition to existing global markets and infrastructure for storage, distribution and utilization in a wide range of applications. Consequently, propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. This study focuses on the construction and evaluation of alternative microbial biosynthetic pathways for the production of renewable propane. The new pathways utilize CoA intermediates that are derived from clostridial-like fermentative butanol pathways and are therefore distinct from the first microbial propane pathways derived from FAS recently engineered in E. coli.
The following embodiments and aspects thereof are described and illustrated in conjunction with systems, compositions and methods which are meant to be exemplary and illustrative, not limiting in scope.
Described herein are the construction and evaluation of novel pathways for butane and propane production in E. coli that are independent of the FAS pathway in a recent study [Kallio P et al., Nature communications 2014, 5:4731], thereby opening up possibilities for further optimization of short chain-length alkane biosynthesis.
Provided herein are genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
Also provided herein are genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
Further provided herein are genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(v) is Escherichia coli.
Also provided herein are genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(vii) is Escherichia coli.
As described herein, genes expressing enzymes for production of propane or butanol may be derived from various microorganisms.
Also provided herein are methods for producing butanol. The methods comprise, consist of or consist essentially of providing the genetically engineered microorganism described herein and culturing the microorganism so as to produce butanol.
Further provided herein are methods for producing propane. The methods comprise, consist of or consist essentially of providing the genetically engineered microorganism described herein and culturing the microorganism so as to produce propane.
The present invention is described in relation to the following clauses:
providing the genetically engineered microorganism of any one of clauses 7 to 13; and
culturing the microorganism, so as to produce butanol.
providing the genetically engineered microorganism of any one of clauses 14 to 24; and
culturing the microorganism, so as to produce propane.
Exemplary embodiments are illustrated in referenced figures. It is intended that the embodiments and figures disclosed herein are to be considered illustrative rather than restrictive.
All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Allen et al., Remington: The Science and Practice of Pharmacy 22nd ed., Pharmaceutical Press (Sep. 15, 2012); Hornyak et al., Introduction to Nanoscience and Nanotechnology, CRC Press (2008); Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology 3rd ed., revised ed., J. Wiley & Sons (New York, N.Y. 2006); Smith, March's Advanced Organic Chemistry Reactions, Mechanisms and Structure 7th ed., J. Wiley & Sons (New York, N.Y. 2013); Singleton, Dictionary of DNA and Genome Technology 3rd ed., Wiley-Blackwell (Nov. 28, 2012); and Green and Sambrook, Molecular Cloning: A Laboratory Manual 4th ed., Cold Spring Harbor Laboratory Press (Cold Spring Harbor, N.Y. 2012), provide one skilled in the art with a general guide to many of the terms used in the present application. For references on how to prepare antibodies, see Greenfield, Antibodies A Laboratory Manual 2nd ed., Cold Spring Harbor Press (Cold Spring Harbor N.Y., 2013); Köhler and Milstein, Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion, Eur. J. Immunol. 1976 July, 6 (7):511-9; Queen and Selick, Humanized immunoglobulins, U.S. Pat. No. 5,585,089 (1996 December); and Riechmann et al., Reshaping human antibodies for therapy, Nature 1988 Mar. 24, 332 (6162):323-7.
One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention. Other features and advantages of the invention will become apparent from the following detailed description, taken in conjunction with the accompanying drawings, which illustrate, by way of example, various features of embodiments of the invention. Indeed, the present invention is in no way limited to the methods and materials described. For convenience, certain terms employed herein, in the specification, examples and appended claims are collected here.
Unless stated otherwise, or implicit from context, the following terms and phrases include the meanings provided below. Unless explicitly stated otherwise, or apparent from context, the terms and phrases below do not exclude the meaning that the term or phrase has acquired in the art to which it pertains. The definitions are provided to aid in describing particular embodiments, and are not intended to limit the claimed invention, because the scope of the invention is limited only by the claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
Unless stated otherwise, the terms “a” and “an” and “the” and similar references used in the context of describing a particular embodiment of the application (especially in the context of claims) can be construed to cover both the singular and the plural. The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (for example, “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the application and does not pose a limitation on the scope of the application otherwise claimed. The abbreviation, “e.g.” is derived from the Latin exempli gratia, and is used herein to indicate a non-limiting example. Thus, the abbreviation “e.g.” is synonymous with the term “for example.” No language in the specification should be construed as indicating any non-claimed element essential to the practice of the application.
As used herein, the term “comprising” or “comprises” is used in reference to compositions, methods, and respective component(s) thereof, that are useful to an embodiment, yet open to the inclusion of unspecified elements, whether useful or not. It will be understood by those within the art that, in general, terms used herein are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.). Although the open-ended term “comprising,” as a synonym of terms such as including, containing, or having, is used herein to describe and claim the invention, the present invention, or embodiments thereof, may alternatively be described using alternative terms such as “consisting of” or “consisting essentially of”
As used herein, the terms “recombinant microorganism” or “genetically engineered microorganism” refers to microorganism having expression of or increased expression of one or more target enzymes relative to the parental microorganism or encode one or more enzymes not found in the parental organism. In an embodiment, the microorganism has reduced expression of or absence of expression of one or more genes encoding an enzyme that competes with a metabolite necessary for the production of a desired product or which produces an undesirable product. The recombinant microorganism produces at least one metabolite involved in a biosynthetic pathway for the production of propane. In general, the recombinant microorganisms comprises at least one recombinant metabolic pathway that comprises a target enzyme and may further include a reduction in activity or expression of an enzyme in a competitive biosynthetic pathway. The pathway acts to modify a substrate or metabolic intermediate in the production of propane, butanol or a combination thereof. The target enzyme is encoded by, and expressed from, a polynucleotide derived from a suitable biological source. In some embodiments, the polynucleotide comprises a gene derived from a bacterial, archaeon, plant, or yeast source and recombinantly engineered into the microorganism described herein.
As used herein, the term “metabolically engineered” or “metabolic engineering” refers to rational pathway design and assembly of biosynthetic genes, genes associated with operons, and control elements of such polynucleotides, for the production of a desired metabolite, such as an acetoacetyl-CoA, in a microorganism. “Metabolically engineered” can further include optimization of metabolic flux by regulation and optimization of transcription, translation, protein stability and protein functionality using genetic engineering and appropriate culture condition including the reduction of, disruption, or knocking out of, a competing metabolic pathway that competes with an intermediate or use of a cofactor or energy source, leading to a desired pathway. A biosynthetic gene can be heterologous to the host microorganism, either by virtue of being foreign to the host, or being modified by mutagenesis, recombination, and/or association with a heterologous expression control sequence in an endogenous host cell. In one embodiment, where the polynucleotide is xenogenetic to the host organism, the polynucleotide can be codon optimized. In an exemplary embodiment, deletion of competing metabolic pathways as applicable herein include deletion of any one or more of aldehyde reductase enzyme, alcohol dehydrogenase enzyme or a combination thereof, in the host organism.
The term “biosynthetic pathway” or “metabolic pathway” refers to anabolic or catabolic biochemical reactions for converting (transforming) one chemical species into another. Gene products belong to the same “metabolic pathway” if they, in parallel or in series, act on the same substrate, produce the same product, or act on or produce a metabolic intermediate (i.e., metabolite) between the same substrate and metabolite end product.
The term “substrate” or “suitable substrate” refers to any substance or compound that is converted or meant to be converted into another compound by the action of an enzyme. The term includes a single compound or combinations of compounds, such as solutions, mixtures and other materials which contain at least one substrate, or derivatives thereof. Further, the term “substrate” encompasses not only compounds that provide a carbon source suitable for use as a starting material, such as any biomass derived sugar, but also intermediate and end product metabolites used in a pathway associated with a metabolically engineered microorganism as described herein.
The “activity” of an enzyme is a measure of its ability to catalyze a reaction resulting in a metabolite, i.e., to “function”, and may be expressed as the rate at which the metabolite of the reaction is produced. For example, enzyme activity can be represented as the amount of metabolite produced per unit of time or per unit of enzyme (e.g., concentration or weight), or in terms of affinity or dissociation constants.
An “enzyme” refers to any substance, typically composed wholly or largely of protein, that catalyzes or promotes, more or less specifically, one or more chemical or biochemical reactions. The term “enzyme” can also refer to a catalytic polynucleotide (e.g., RNA or DNA).
The term “polynucleotide”, “nucleic acid” or “recombinant nucleic acid” refers to polynucleotides such as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleic acid (RNA). A “vector” generally refers to a polynucleotide that can be propagated and/or transferred between organisms, cells, or cellular components. Vectors include viruses, bacteriophage, pro-viruses, plasmids, phagemids, transposons, and artificial chromosomes such as YACs (yeast artificial chromosomes), BACs (bacterial artificial chromosomes), and PLACs (plant artificial chromosomes), and the like, that are “episomes” that is, that replicate autonomously or can integrate into a chromosome of a host cell. A vector can also be a naked RNA polynucleotide, a naked DNA polynucleotide, a polynucleotide composed of both DNA and RNA within the same strand, a poly-lysine-conjugated DNA or RNA, a peptide-conjugated DNA or RNA, a liposome-conjugated DNA, or the like, that are not episomal in nature, or it can be an organism which comprises one or more of the above polynucleotide constructs such as an agrobacterium or a bacterium.
A “native” or “wild-type” protein, enzyme, polynucleotide, gene, or cell, means a protein, enzyme, polynucleotide, gene, or cell that occurs in nature.
“Transformation” refers to the process by which a vector is introduced into a host cell. Transformation (or transduction, or transfection), can be achieved by any one of a number of means including electroporation, microinjection, biolistics (or particle bombardment-mediated delivery), or agrobacterium mediated transformation.
The inventors herein report new pathways for propane production based on a fermentative butanol pathway. These are distinct from previously reported pathways that were based on fatty acid synthesis, where propane production is limited by the availability of butyraldehyde precursors and the poor activity of aldehyde deformylating oxygenase (ADO) with butyraldehyde. Herein, the inventors demonstrate that the new pathways are plausible alternatives for the construction of next-generation microbial propane production platforms.
Recombinant Microorganisms
The pathways for propane and butanol production are depicted in
Accordingly, provided herein are genetically engineered microorganisms that can express a plurality of enzymes involved in the pathway for production of propane, butanol or a combination thereof, from a suitable carbon source. Exemplary carbon sources include but are not limited to glucose, glycerol, complex feedstocks (e.g. hydrolysates). In one embodiment, the enzymes are involved in the metabolic pathway for production of propane. The genetically engineered microorganisms described herein produce metabolites in quantities that are higher than the parent microorganism. Examples of metabolites include starting metabolites (such as glucose or glycerol), intermediates (such as acetoacetyl-CoA) and/or end products (such as propane or butanol).
The genetically engineered microorganisms described herein are produced by introduction of genetic material into the parent (host) microorganism so that the microorganism acquires new properties which are not present in the parental microorganism. In exemplary embodiments, new properties include abilities to produce or produce in increased amounts, metabolic enzymes involved in the pathways to produce propane, butanol or a combination thereof. The genetic material may additionally include elements that regulate synthesis of the metabolic enzymes including but not limited to promoter sequences and sequences specific for inducers.
In one embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
In another embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
In a further embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(v) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
In a further embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In one embodiment, the host/parent organism engineered to express enzymes (i)-(v) and aldehyde deformylating oxygenase (Ado) is Escherichia coli. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
As described herein, in some embodiments, the genetically engineered microorganisms for propane production express wild type aldehyde deformylating oxygenase (for example, ado). In some embodiments, the genetically engineered microorganisms for propane production express mutant form of aldehyde deformylating oxygenase. In an embodiment, the mutation in aldehyde deformylating oxygenase results in increased production of propane. In an exemplary embodiment, the genetically engineered microorganisms for propane production comprises a polynucleotide encoding the polypeptide having aldehyde deformylating oxygenase activity comprises, consists of or consists essentially of an amino acid substitution at position 134 from alanine to phenylalanine.
In some embodiments, the genetically engineered microorganisms for propane production described herein further comprise iron-sulfur proteins (for example, ferredoxin PetF) having electron transfer activity so as to increase supply of electrons to ADO, thereby increasing propane production.
In some embodiments, the parent of the genetically engineered microorganism for propane production comprises deletion of competing pathways so as to increase propane production. In an exemplary embodiment, deletion of any one or more of aldehyde reductase enzyme (for example, deletion of ahr (Δahr)), alcohol dehydrogenase enzyme (for example, deletion of yqhD (ΔyqhD)) or a combination thereof, increases propane production.
In an embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In an embodiment, the microorganisms further express polynucleotides encoding ferredoxins (for example, PetF). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii), aldehyde deformylating oxygenase (Ado) and PetF is Escherichia coli. In an embodiment, the host organism includes deletion of any one or more of aldehyde reductase enzyme (for example, deletion of ahr (Δahr), alcohol dehydrogenase enzyme (for example, deletion of yqhD (ΔyqhD) or a combination thereof. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
In another embodiment, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In an embodiment, the microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde deformylating oxygenase activity (for example, ado). In an embodiment, the microorganisms further express polynucleotides encoding ferredoxins (for example, PetF). In one embodiment, the host/parent organism engineered to express enzymes (i)-(vii), aldehyde deformylating oxygenase (Ado) and PetF is Escherichia coli. In an embodiment, the host organism includes deletion of any one or more of aldehyde reductase enzyme (for example, deletion of ahr (Δahr)), alcohol dehydrogenase enzyme (for example, deletion of yqhD (ΔyqhD)) or a combination thereof. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor.
In an embodiment, genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(v) is Escherichia coli.
In another embodiment, genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(vii) is Escherichia coli.
In a further embodiment, genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(v) is Escherichia coli.
In another embodiment, genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp) and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(vii) is Escherichia coli.
As described herein, each enzyme produced by the genetically engineered microorganisms described herein produce metabolites. In one embodiment, the genetically engineered microorganisms described herein express a thiolase to produce the metabolite acetoacetyl-CoA from two molecules of acetyl-CoA. In one embodiment, the thiolase is encoded by an atoB gene or a homolog thereof and encodes the polypeptide having acetyl-CoA acetyltransferase activity. The atoB gene may be derived from any biological source including but not limited to Escherichia coli, Clostridium acetobutylicum Klebsiella pneumonia, Pseudomonas fluorescens F113, Helicobacter acinonychis st. Sheeba and Desulfobacterium autotrophicum.
In another embodiment, the genetically engineered microorganisms described herein express an acetoacetyl-CoA synthase to produce the metabolite acetoacetyl-CoA from acetyl-CoA and malonyl-CoA. In an exemplary embodiment, the acetoacetyl-CoA synthase is encoded by an nphT7 gene or a homolog thereof and can be derived from any biological source including but not limited to Streptomyces sp. CL190, Paenibacillus sp. P1XP2, Legionella pneumophila and Microbacterium ketosireducens.
In a further embodiment, genetically engineered microorganisms described herein express oxidoreductases such as 3-hydroxybutyryl-CoA dehydrogenase to produce the metabolite 3-hydroxybutyryl-CoA from a substrate that includes acetoacetyl-CoA. In an exemplary embodiment, the 3-hydroxybutyryl-CoA dehydrogenase is encoded by an hbd gene or homolog thereof and can be derived from any biological source including but not limited to Clostridium acetobutylicum, Clostridium difficile, Butyrivibrio fibrisolvens, Treponema phagedenis, Acidaminococcus fermentans, Clostridium kluyveri, and Thermoanaerobacterium thermosaccharolyticum. In one embodiment, the hbd gene is derived from Clostridium acetobutylicum.
In an additional embodiment, genetically engineered microorganisms described herein express lyases (for example, hydro-lyases) such as 3-hydroxybutyryl-CoA dehydratase to produce the metabolite crotonoyl-CoA from a substrate that includes 3-hydroxybutyryl-CoA. In an exemplary embodiment, the 3-hydroxybutyryl-CoA dehydratase is encoded by a crt gene or a homolog thereof and can be derived from any biological source including but not limited to Clostridium acetobutylicum, Butyrivibrio fibrisolvens, Thermoanaerobacterium thermosaccharolyticum, Methylobacterium extorquens, Dictyostelium discoideum, Clostridium pasteurianum, Mycobacterium smegmatis and Clostridium difficile. In one embodiment, the crt gene is derived from Clostridium acetobutylicum.
In a further embodiment, genetically engineered microorganisms described herein express additional oxidoreductases such as trans-2-enoyl-CoA reductase (TER) to produce the metabolite butyryl-CoA from a substrate that includes crotonoyl-CoA. In an exemplary embodiment, the trans-2-enoyl-CoA reductase is encoded by the ter gene or a homolog thereof and can be derived from any biological source including but not limited to Treponema denticola, Clostridium acetobutylicum, Euglena gracilis, Paenibacillus sp. FSL R5-808 and Fibrobacter succinogenes. In one embodiment, the ter gene is derived from Treponema denticola.
In another embodiment, genetically engineered microorganisms described herein express hydrolases (for example, those acting on thioester bonds) such as acyl-CoA thioester hydrolase to produce the metabolite butyric acid from a substrate that includes butyryl-CoA. In an exemplary embodiment, the acyl-CoA thioester hydrolase is encoded by the yciA or Acot gene or a homolog thereof and can be derived from any biological source including but not limited to Campylobacter jejuni, Haemophilus influenza, Escherichia coli, Rattus norvegicus, Deinococcus peraridilitoris, Chlamydia pneumonia, Shigella flexneri, Zymomonas mobilis subsp. Mobilis, Enterobacter cloacae and Acinetobacter sp. ADP1. In one embodiment the yciA gene is derived from Haemophilus influenza.
In a further embodiment, genetically engineered microorganisms described herein express additional oxidoreductases (for example, those acting on aldehyde groups) such as carboxylic acid reductase to produce the metabolite butyraldehyde from a substrate that includes butyric acid. In an exemplary embodiment, the carboxylic acid reductase is encoded by the car gene or a homolog thereof and can be derived from any biological source including but not limited to Mycobacterium marinum, Streptomyces sp. W007, Tolypocladium ophioglossoides CBS 100239, Mycobacterium obuense and Nocardia iowensis. In one embodiment the yciA gene is derived from Mycobacterium marinum.
In another embodiment, genetically engineered microorganisms described herein express alcohol dehydrogenases such as aldehyde-alcohol dehydrogenase to produce the metabolite butanol from a substrate that includes butyryl-CoA. In an exemplary embodiment, the alcohol dehydrogenase can be encoded by bdhA/bdhB gene or homolog thereof, an aad gene, or homolog thereof, or an adhE2 gene or homolog thereof. The aad gene or adhE2 can be derived from Clostridium acetobutylicum.
In an embodiment, genetically engineered microorganisms described herein express aldehyde deformylating oxygenase (ADO) to produce the metabolite propane from the substrate butyraldehyde. In an exemplary embodiment, aldehyde deformylating oxygenase is derived from Prochlorococcus marinus.
In an embodiment, genetically engineered microorganisms described herein express iron-sulfur proteins so as to increase electron supply and thereby increase ADO-dependent propane production. In an exemplary embodiment, the iron-sulfur protein is ferredoxin PetF or a homolog thereof and may be derived from any biological source including but not limited to Synechocystis sp. PCC 6803, Thermosynechococcus elongatus st. BP-1, Acaryochloris marina st. MBIC 11017, Roseobacter litoralis, Planktothrix agardhii, Octadecabacter arcticus 238 and Nostoc sp. strain ATCC 29151.
The instant disclosure identifies genes useful in the methods, compositions and organisms of the disclosure. However it will be recognized that absolute identity to such genes is not necessary. For example, changes in a particular gene or polynucleotide comprising a sequence encoding a polypeptide or enzyme can be performed and screened for activity. Typically such changes comprise conservative mutation and silent mutations. Such modified or mutated polynucleotides and polypeptides can be screened for expression of a functional enzyme activity using methods known in the art.
In addition, homologs of enzymes useful for generating metabolites (such as propane and/or butanol) are encompassed by the microorganisms and methods provided herein. The term “homologs” used with respect to an original enzyme or gene of a first family or species refers to distinct enzymes or genes of a second family or species which are determined by functional, structural or genomic analyses to be an enzyme or gene of the second family or species which corresponds to the original enzyme or gene of the first family or species. Most often, homologs will have functional, structural or genomic similarities. Techniques are known by which homologs of an enzyme or gene can readily be cloned using genetic probes and PCR. Identity of cloned sequences as homolog can be confirmed using functional assays and/or by genomic mapping of the genes.
In some embodiments, the amount of propane produced by the genetically engineered microorganisms is any one or more of at least about 100 μg/L, at least about 200 μg/L, at least about 300 μg/L, at least about 400 μg/L, at least about 500 μg/L, at least about 600 μg/L, at least about 700 μg/L, at least about 800 μg/L, at least about 900 μg/L, at least about 1 mg/L, at least about 2 mg/L, at least about 3 mg/L, at least about 4 mg/L, at least about 5 mg/L, at least about 6 mg/L, at least about 7 mg/L, at least about 8 mg/L, at least about 9 mg/L, at least about 10 mg/L or combinations thereof, in, for example, 72 hours. In some embodiments, propane production by the genetically engineered microorganisms is higher in the presence of any one or more of deletion of competing pathways, ADOA134F, ferredoxin-based electron supply system or a combination thereof.
In some embodiments, the amount of butanol produced by the genetically engineered microorganisms is any one or more of at least about 100 mg/L, at least about 200 mg/L, at least about 300 mg/L, at least about 400 mg/L, at least about 500 mg/L, at least about 600 mg/L, at least about 700 mg/L, at least about 800 mg/L, at least about 900 mg/L, at least about 1000 mg/L or combinations thereof, in, for example, 72 hours.
Methods
Provided herein are methods for producing butanol. The methods comprise, consist of or consist essentially of providing the genetically engineered microorganism described herein and culturing the microorganism so as to produce butanol.
In one embodiment of the method for producing butanol, the genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms for synthesis of butanol further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (Ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(vii) is Escherichia coli.
In another embodiment of the method for producing butanol, genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms for synthesis of butanol further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (Ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(v) is Escherichia coli.
In a further embodiment of the method for producing butanol, genetically engineered microogranisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and (v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (Ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(v) is Escherichia coli.
In a further embodiment of the method for producing butanol, genetically engineered microogranisms for synthesis of butanol express or increase expression of a polynucleotide encoding a polypeptide having (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms for synthesis of butanol further express or increase expression of a polynucleotide encoding a polypeptide having aldehyde reductase (Ahr) activity. In one embodiment, the host organism engineered to express enzymes (i)-(vii) is Escherichia coli.
Provided herein are methods for producing propane. The methods comprise, consist of or consist essentially of providing the genetically engineered microorganism described herein and culturing the microorganism so as to produce propane. In one embodiment of the method for producing propane, the genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) the activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms for synthesis of propane further express or increase expression of aldehyde deformylating oxygenase activity (for example, ado). In some embodiments, the genetically engineered microorganism further encodes a ferredoxin so as to increase electron supply and thus increase propane production. In some embodiments, the parent of the genetically engineered microorganism comprises deletion of one or more of aldehyde reductase enzyme (for example, deletion of ahr), alcohol dehydrogenase enzyme (for example, deletion of yqhD) or a combination thereof, so as to increase the amount of propane synthesized. In some embodiments, aldehyde deformylating oxygenase (for example, ado) is wild type. In some embodiments, aldehyde deformylating oxygenase is a mutant, wherein the mutation results in increased production of propane. In an exemplary embodiment, the polynucleotide encoding the polypeptide having aldehyde deformylating oxygenase activity comprises, consists of or consists essentially of an amino acid substitution at position 134 from alanine to phenylalanine. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor. In one embodiment, the host organism engineered to express enzymes (i)-(viii) is Escherichia coli.
In another embodiment of the method for producing propane, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); (v) acyl-CoA thioester hydrolase activity (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase activity (for example, car). In some embodiments, the genetically engineered microorganisms for synthesis of propane further express or increase expression of aldehyde deformylating oxygenase activity (for example, ado). In some embodiments, the genetically engineered microorganism further encodes a ferredoxin so as to increase electron supply and thus increase propane production. In some embodiments, the parent of the genetically engineered microorganism comprises deletion of one or more of aldehyde reductase enzyme (for example, deletion of ahr), alcohol dehydrogenase enzyme (for example, deletion of yqhD) or a combination thereof, so as to increase the amount of propane synthesized. In some embodiments, aldehyde deformylating oxygenase (for example, ado) is wild type. In some embodiments, aldehyde deformylating oxygenase is a mutant, wherein the mutation results in increased production of propane. In an exemplary embodiment, the polynucleotide encoding the polypeptide having aldehyde deformylating oxygenase activity comprises, consists of or consists essentially of an amino acid substitution at position 134 from alanine to phenylalanine. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor. In one embodiment, the host organism engineered to express enzymes (i)-(viii) is Escherichia coli.
In another embodiment of the method for producing propane, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetyl-CoA acetyltransferase activity (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms for synthesis of propane further express or increase expression of aldehyde deformylating oxygenase activity (for example, ado). In some embodiments, the genetically engineered microorganism further encodes a ferredoxin so as to increase electron supply and thus increase propane production. In some embodiments, the parent of the genetically engineered microorganism comprises deletion of one or more of aldehyde reductase enzyme (for example, deletion of ahr), alcohol dehydrogenase enzyme (for example, deletion of yqhD) or a combination thereof, so as to increase the amount of propane synthesized. In some embodiments, aldehyde deformylating oxygenase (for example, ado) is wild type. In some embodiments, aldehyde deformylating oxygenase is a mutant, wherein the mutation results in increased production of propane. In an exemplary embodiment, the polynucleotide encoding the polypeptide having aldehyde deformylating oxygenase activity comprises, consists of or consists essentially of an amino acid substitution at position 134 from alanine to phenylalanine. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor. In one embodiment, the host organism engineered to express enzymes (i)-(viii) is Escherichia coli.
In another embodiment of the method for producing propane, genetically engineered microorganisms for synthesis of propane express or increase expression of a polynucleotide encoding a polypeptide having: (i) acetoacetyl-CoA synthase activity (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase activity (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase activity (for example, crt); (iv) trans-2-enoyl-CoA reductase activity (for example, ter); and v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In some embodiments, the genetically engineered microorganisms for synthesis of propane further express or increase expression of aldehyde deformylating oxygenase activity (for example, ado). In some embodiments, the genetically engineered microorganism further encodes a ferredoxin so as to increase electron supply and thus increase propane production. In some embodiments, the parent of the genetically engineered microorganism comprises deletion of one or more of aldehyde reductase enzyme (for example, deletion of ahr), alcohol dehydrogenase enzyme (for example, deletion of yqhD) or a combination thereof, so as to increase the amount of propane synthesized. In some embodiments, aldehyde deformylating oxygenase (for example, ado) is wild type. In some embodiments, aldehyde deformylating oxygenase is a mutant, wherein the mutation results in increased production of propane. In an exemplary embodiment, the polynucleotide encoding the polypeptide having aldehyde deformylating oxygenase activity comprises, consists of or consists essentially of an amino acid substitution at position 134 from alanine to phenylalanine. In an embodiment, propane synthesis set forth herein is independent of aldehyde-alcohol dehydrogenase and fatty acid synthesis. In some embodiments, propane is produced by utilizing butyraldehyde as a precursor. In one embodiment, the host organism engineered to express enzymes (i)-(viii) is Escherichia coli.
Also provided herein are methods for producing a microorganism capable of producing propane. In an embodiment, the methods comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding acetyl-CoA acetyltransferase (AtoB), 3-hydroxybutyrl-CoA dehydrogenase (Hbd), 3-hydroxybutyryl-CoA dehydratase (Crt), trans-2-enoyl-CoA reductase (Ter), acyl-CoA thioester hydrolase (YciA), maturation factor for phosphopantetheinyl transferase (Sfp) and carboxylic acid reductase (CAR) activity. In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding a ferredoxin so as to increase electron supply and thus increase propane production. In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding any one or more of aldehyde deformylating oxygenase (ADO) or aldehyde deformylating oxygenase activity wherein alanine at position 134 is substituted with phenylalanine (ADOA134F) or a combination thereof. In an additional embodiment, the method includes deleting nucleotides encoding competing pathways (for example, deleting nucleotides encoding polypeptides having aldehyde reductase activity, alcohol dehydrogenase activity or a combination thereof).
In another embodiment, the methods for producing microorganisms capable of producing propane comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetoacetyl-CoA synthase (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase (for example, crt); (iv) trans-2-enoyl-CoA reductase (for example, ter); (v) acyl-CoA thioester hydrolase (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase (for example, car). In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding a ferredoxin so as to increase electron supply and thus increase propane production. In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding any one or more of aldehyde deformylating oxygenase (ADO) or aldehyde deformylating oxygenase activity wherein alanine at position 134 is substituted with phenylalanine (ADOA134F) or a combination thereof. In an additional embodiment, the method includes deleting nucleotides encoding competing pathways (for example, deleting nucleotides encoding polypeptides having aldehyde reductase activity, alcohol dehydrogenase activity or a combination thereof).
In a further embodiment, the methods for producing microorganisms capable of producing propane comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetyl-CoA acetyltransferase (for example, atoB); (ii) 3-hydroxybutyrl-CoA dehydrogenase (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase (for example, crt); (iv) trans-2-enoyl-CoA reductase (for example, ter); and v) aldehyde-alcohol dehydrogenase activity (for example, adhE2). In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding a ferredoxin so as to increase electron supply and thus increase propane production. In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding any one or more of aldehyde deformylating oxygenase (ADO) or aldehyde deformylating oxygenase activity wherein alanine at position 134 is substituted with phenylalanine (ADOA134F) or a combination thereof. In an additional embodiment, the method includes deleting nucleotides encoding competing pathways (for example, deleting nucleotides encoding polypeptides having aldehyde reductase activity, alcohol dehydrogenase activity or a combination thereof).
In an embodiment, the methods for producing microorganisms capable of producing propane comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetoacetyl-CoA synthase (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase (for example, crt); (iv) trans-2-enoyl-CoA reductase (for example, ter); and v) aldehyde-alcohol dehydrogenase (for example, adhE2). In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding a ferredoxin so as to increase electron supply and thus increase propane production. In one embodiment, the method further includes transforming a microorganism with polynucleotides encoding any one or more of aldehyde deformylating oxygenase (ADO) or aldehyde deformylating oxygenase activity wherein alanine at position 134 is substituted with phenylalanine (ADOA134F) or a combination thereof. In an additional embodiment, the method includes deleting nucleotides encoding competing pathways (for example, deleting nucleotides encoding polypeptides having aldehyde reductase activity, alcohol dehydrogenase activity or a combination thereof).
Also provided herein are methods for producing a microorganism capable of producing butanol. In one embodiment, the methods comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetyl-CoA acetyltransferase (AtoB), (ii) 3-hydroxybutyrl-CoA dehydrogenase (Hbd), (iii) 3-hydroxybutyryl-CoA dehydratase (Crt), (iv) trans-2-enoyl-CoA reductase (Ter), and (v) aldehyde-alcohol dehydrogenase (AdhE2). In some embodiments, the methods include transforming the microorganism with polynucleotides encoding aldehyde reductase (Ahr).
In another embodiment, the methods for producing a microorganism capable of producing butanol comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetyl-CoA acetyltransferase (AtoB), (ii) 3-hydroxybutyrl-CoA dehydrogenase (Hbd), (iii) 3-hydroxybutyryl-CoA dehydratase (Crt), (iv) trans-2-enoyl-CoA reductase (Ter), (v) acyl-CoA thioester hydrolase (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase (for example, car). In some embodiments, the methods include transforming the microorganism with polynucleotides encoding aldehyde reductase (Ahr).
In a further embodiment, the methods for producing a microorganism capable of producing butanol comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetoacetyl-CoA synthase (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase (for example, crt); (iv) trans-2-enoyl-CoA reductase (for example, ter); (v) acyl-CoA thioester hydrolase (for example, yciA); (vi) activity of a maturation factor for phosphopantetheinyl transferase (for example, sfp); and (vii) carboxylic acid reductase (for example, car). In some embodiments, the methods include transforming the microorganism with polynucleotides encoding aldehyde reductase (Ahr).
In another embodiment, the methods for producing a microorganism capable of producing butanol comprise, consist of or consist essentially of transforming a microorganism with polynucleotides encoding (i) acetoacetyl-CoA synthase (for example, nphT7); (ii) 3-hydroxybutyrl-CoA dehydrogenase (for example, hbd); (iii) 3-hydroxybutyryl-CoA dehydratase (for example, crt); (iv) trans-2-enoyl-CoA reductase (for example, ter), and (v) aldehyde-alcohol dehydrogenase (AdhE2). In some embodiments, the methods include transforming the microorganism with polynucleotides encoding aldehyde reductase (Ahr).
In some embodiments, the vectors in the genetically engineered microorganism encoding enzymes comprise an inducible promoter so as to increase synthesis of the enzymes. In exemplary embodiments, the promoters are isopropyl β-D-1-thiogalactopyranoside (IPTG) or arabinose inducible.
The following examples are not intended to limit the scope of the claims to the invention, but are rather intended to be exemplary of certain embodiments. Any variations in the exemplified methods which occur to the skilled artisan are intended to fall within the scope of the present invention.
Herein, the inventors report the assembly and evaluation of four different synthetic pathways for the production of propane and butanol, designated a) atoB-adhE2 route), b) atoB-TPC7 route, c) nphT7-adhE2 route and d) nphT7-TPC7 route. The highest butanol titres were achieved with the atoB-adhE2 (473±3 mg/L) and atoB-TPC7-Ahr (163±2 mg/L) routes. When aldehyde deformylating oxygenase (ADO) was co-expressed with these pathways the engineered hosts also produced propane. The atoB-TPC7-ADO pathway was the most effective in producing propane (220±3 μg/L). By (i) deleting competing pathways, (ii) including a previously designed ADOA134F variant with an enhanced specificity towards short-chain substrates, and (iii) including and ferredoxin-based electron supply system, the propane titre was increased (3.40±0.19 mg/L).
This study expands the metabolic toolbox for renewable propane and butanol production and provides new insight and understanding for the development of next-generation biofuel platforms. In developing an alternative CoA dependent fermentative butanol pathway, which includes an engineered ADO variant (ADOA134F), the study addresses known limitations, including the low bio-availability of butyraldehyde precursors and poor activity of ADO with butyraldehyde.
Experimental Methods
All chemicals, solvents and standards were purchased from Sigma-Aldrich and Fisher Scientific, and were of analytical grade. Media components were obtained from Formedium (Norfolk, UK). Gene sequencing and oligonucleotide synthesis were performed by Eurofins MWG (Ebersberg, Germany). D-Glucose (GOPOD Format) assay kit was from Megazyme.
Strains and Plasmids. BL21 (DE3) (fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS) cells from Novagen were used for protein expression. The ahr (GenBank ID: ACT44688.1) and yqhD (GenBank ID: AAA97166.1) single and double knockout strains were generated in a previous study [Kallio P et al. Nature communications 2014, 5:4731]. The structure of all plasmids is graphically depicted in
pET-TPC7. The pET-TPC7 plasmid was constructed in a pETDuetT-1 vector (ColE1 replicon, AmpicillinR, from Novagen) by replacing TE4 acyl-ACP thioesterase with a synthesized gene encoding YciA (Haemophilus influenza, GenBank ID: AAC22485.1) in pET-TPC4 backbone plasmid described in the previous study using restriction sites HindIII and NcoI [Kallio P et al. Nature communications 2014, 5:4731]. The final plasmid contained synthesized genes encoding Sfp (maturation factor phosphopantetheinyl transferase from Bacillus subtilis, GenBank ID: X65610.1), and CAR (carboxylic acid reductase from Mycobacterium marinum, GenBank ID: ACC40567.1) upstream to yciA.
pACYC-NHCT. The pACYC-NHCT plasmid was constructed in a pACYCDuet-1 vector (P15A replicon, ChloramphenicolR, from Novagen) by subcloning a synthesized NcoI-BamHI gene fragment (GenScript, USA) carrying genes encoding NphT7 (acetoacetyl-CoA synthase from Streptomyces sp. CL190, GenBank ID: D7URV0.1) and Hbd (3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824, GenBank ID: P52041.2) into a synthesized pACYC construct harbouring crt (3-hydroxybutyryl-CoA dehydratase from Clostridium acetobutylicum ATCC 824, GenBank ID: P52046.1) and ter (trans-2-enoyl-CoA reductase from Treponema denticola ATCC 35405, GenBank ID: Q73Q47.1).
pACYC-AHCT. pACYC-AHCT is a pACYCDuet-1 vector (P15A replicon, ChloramphenicolR, from Novagen) with atoB (acetyl-CoA acetyltransferase from E. coli, GenBank ID: P76461.1), hbd (3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824, GenBank ID: P52041.2), crt (3-hydroxybutyryl-CoA dehydratase from Clostridium acetobutylicum ATCC 824, GenBank ID: P52046.1) and ter (trans-2-enoyl-CoA reductase from Treponema denticola ATCC 35405, GenBank ID: Q73Q47.1) genes inserted in the order. Gene atoB (acetyl-CoA acetyltransferase from E. coli, GenBank ID: P76461.1) was PCR-amplified from E. coli K-12 purified genome, using primers 5′-attaggtaccAAAAATTGTGTCATCGTCAGTGCGGTAC (SEQ ID NO:1) and 5′-attaaagcTTAATTCAACCGTTCAATCACCATCGCAAT (SEQ ID NO:2), showing complementary regions in capital letters. The atoB PCR fragment was then subcloned into pACYC-NHCT, thus replacing the KpnI-HindIII fragment carrying NphT7, resulting pACYC-AHCT plasmid.
pET-AdhE2. The pET-AdhE2 plasmid was constructed in a pET-Duet vector (fl origin, AmpicillinR, from Novagen) with adhE2 by subcloning synthesized gene encoding AdhE2 (aldehyde-alcohol dehydrogenase, from Clostridium acetobutylicum ATCC 824, GenBank ID: Q9ANR5) from a pUC57 parent vector provided by GenScript (USA) into pET-Duet vector, using restriction sites NcoI and AvrII.
pCDF-ADO and pCDF-ADOA134F. The pCDF-ADO plasmid was constructed in a pCDFDuet-1 vector (CDF replicon, streptomycin/spectinomycinR, from Novagen) with ADO (aldehyde deformylating oxygenase from Prochlorococcus marinus MIT9313, GenBank ID: Q7V6D4.1) cloned into the vector using NcoI and EcoRI restriction sites. The pCDF-ADOA134F vector was created by mutating the ADO insert, using A134F_forward (5′-GCA TTT GCG ATT TCT TTT TAT CAT ACG TAC-3′) (SEQ ID NO:3) and A134F reverse primers (5′-GTA CGT ATG ATA AAA AGA AAT CGC AAA TGC-3′) (SEQ ID NO:4). The correct mutations were confirmed by complete plasmid DNA sequencing. Gene encoding ADO was originally provided by E. Neil G. Marsh (Department of Biological Chemistry, University of Michigan, USA) in a pET28b-cAD vector which was used for the previous study [Eser B E et al. Biochemistry-Us 2011, 50:10743-10750].
pCDF-Ahr. The pCDF-Ahr plasmid was constructed in a pCDFDuet-1 vector (CDF replicon, streptomycin/spectinomycinR, from Novagen). Gene encoding Ahr (aldehyde reductase from E. coli GenBank ID: P27250.2) was PCR-amplified from isolated E. coli K-12 genomic DNA, using primers 5′ ATTAATCCATGGTCTAGATAATTAATGGATCCAGGAGGAAACATATGTCGATGATAAAAAGCTATGCCGCAAAAG-3′ (SEQ ID NO:5) and 5′-ATTAATCCTAGGAAGCTTCTCGAGTCAAAAATCGGCTTTCAACACCACGCGG-3′ (SEQ ID NO:6), and cloned into using restriction sites NcoI and AvrII [Akhtar M K et al. Proc Natl Acad Sci USA 2013, 110:87-92].
pRSF-PetF. The pRSF-PetF plasmid was constructed in a pRSF-Duet1 vector (RSF replicon, KanamycinR, from Novagen) with fdx (ferredoxin from Synechocystis sp PCC 6803, GenBank ID: WP_010873424.1) by subcloning synthesized gene from a pUC57 parent vector provided by GenScript (USA) into a pRSF-Duet1 vector using NcoI and AvrII restriction sites.
Co-Expression and Introduction of the Pathway in E. coli
The atoB-adhE2 route was introduced into E. coli or the knockout cells by co-expressing pACYC-AHCT and pET-AdhE2 vectors. The atoB-TPC7 route was introduced into E. coli or the knockout cells by co-expressing pACYC-AHCT and pET-TPC7 vectors. The nphT7-adhE2 route was introduced into E. coli or the knockout cells by co expressing pACYC-NHCT and pET-AdhE2 vectors. The nphT7-TPC7 route was introduced into E. coli or the knockout cells by co-expressing pACYC-NHCT and pET-TPC7 vectors in the cells.
E. coli cells containing engineered pathways were further engineered by co-transforming either pCDF-ADO or pCDF-ADOA134F vectors in order to introduce ADO or the ADOA134F variant. The pRSF-PetF vector was co-expressed for Fdx, while pCDF-Ahr was used to introduce Ahr enzyme in the pathway. The presence of all the proteins in each individual plasmid was confirmed by SDS-PAGE and mass spectrometry analysis of the respective SDS-PAGE bands. In the case of proteins with a hexahistidine tag, Western blotting (using WesternBreeze Chemiluminescent Immunodetection kit from Invitrogen) was also used to analyse the expression of his-tagged proteins.
SDS-PAGE Analysis of Protein Expression in Cells Containing Plasmids Encoding Pathway Components
Protein expression levels in cells containing plasmids that encode the pathway enzymes were examined by SDS-PAGE. T5 media (20 mL; 12 g tryptone, 24 g yeast extract, 4 mL glycerol, 12.5 g K2HPO4, 2.3 g KH2PO4, 20 g glucose per liter) was inoculated with 1% (v/v) transformed E. coli cells and incubated at 37° C. (180 rpm) until the optical density at 600 nm (O.D600 nm) reached 0.5. Cultures were then induced with isopropyl β-D-1-thiogalactopyranoside (IPTG; final concentration of 0.5 mM). Cultures were grown for a further 24 hours at 30° C. (180 rpm). Samples (200 μL) from the cultures were taken for SDS PAGE analysis. Samples were taken before IPTG induction and after 4 or 24 hours of IPTG induction and cells harvested by centrifugation. Samples were electrophoresed in 12% RunBlue precast SDS-PAGE gels (Expedeon, Cambridge, UK). Protein bands were visualized by staining with Instant Blue protein stain (Expedeon).
Media, Cultivation and Detection of Propane and Butanol
Lysogeny broth (LB) liquid media (10 mL) was inoculated using E. coli glycerol stocks (from −80° C.) and incubated at 37° C. overnight at 180 rpm. 50 mL of T5 media (12 g tryptone, 24 g yeast extract, 4 mL glycerol, 12.5 g K2HPO4, 2.3 g KH2PO4, 20 g glucose per liter) was inoculated with 1% (v/v) of the inoculum and kept for incubation at 37° C. (180 rpm) until the optical density at 600 nm (O.D600) reached 0.5. The cultures were then induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM. The cell cultures were further grown for 4 hours at 30° C. and 180 rpm to prepare the samples for propane detection. In the case of total butanol produced in the butanol pathway the culture was left at 30° C. (180 rpm) for 72 hours, while for residual butanol detection samples were taken from this culture when O.D600 reached 1.5. Control cultures were made using untransformed E. coli strain BL21 DE3 cells. For propane formation analysis, 50 mL cell culture was centrifuged at 4000 rpm and the supernatant was discarded. The cell pellets were resuspended in a 6.25 mL T5 media with 0.5 mM IPTG and 500 μL of the resuspended culture was transferred into 2 mL crimp sealed GC vial and used for propane analysis. The vials were incubated at 30° C., with shaking at 180 rpm for 3 hrs. 1.0 mL headspace from the cultures grown in the GC vial was manually removed and injected into the GC with a gas tight syringe. Propane detection was carried out using a Varian 3800 GC equipped with a DB-WAX column (30 m×0.32 mm×0.25 μM film thickness, JW Scientific). The injector temperature was 250° C. with a split ratio of 10:1. The column temperature was set from 40° C. hold for 2 min to 100° C. at 20° C./min with helium flow at 1 mL/min and FID temperature at 250° C. Propane peak was identified by comparing it with the analytical propane standard and quantification was done using a propane calibration curve.
For residual butanol detection 50 mL liquid culture was spun down at 4000 rpm for 10 min. 500 μL of the supernatant was extracted with 500 μL of ethyl acetate containing 0.2% hexane as internal standard and dried over MgSO4. 1 μL sample was analysed in GC using a Varian 3800 GC equipped with a HP-5 column (30 m×0.32 mm×0.25 μM film thickness, JW Scientific). The injector temperature was 250° C. with a split ratio of 20:1. The column temperature was set from 40° C. hold for 1 min to 280° C. at 20° C./min with helium flow at 1 mL/min and FID temperature at 250° C. Butanol peak was identified by comparing with the analytical butanol standard and quantification was done using a butanol calibration curve.
Synthesis of Butyraldehyde Based on the CoA-dependent 1-butanol Pathway
For propane production, the ADO enzyme requires butyraldehyde as a precursor. We therefore constructed two biosynthetic pathways for butyraldehyde synthesis based on the fermentation pathway of 1-butanol, as summarized in
Modification of the Butyraldehyde Pathway by Replacement of AdhE2
In the clostridial pathway, butyryl-CoA is converted to butanol in two successive catalytic steps by the bifunctional aldehyde/alcohol dehydrogenase AdhE2 (
Evaluation of Pathway Routes for Propane Synthesis
The combined analysis clearly indicated that all pathway components for butyraldehyde synthesis were functional. In order to convert butyraldehyde to propane, the aldehyde reductases in the four butanol pathways were thereafter replaced or complemented by ADO. The TPC7-based strains produced relatively more propane (atoB-TPC7-ADO and nphT7-TPC7-ADO) compared to other strains (
The two functional TPC7-dependent propane pathways were subsequently further modified by replacing ADO with the ADOA134F variant enzyme (
Increasing Electron Supply to ADO Via Ferredoxin
It has previously been shown that the supply of electrons to ADO via endogenous enzymes was inadequate for effective alkane biosynthesis in E. coli [Kallio P et al. Nature communications 2014, 5:4731]. To alleviate this constraint, the heterologous ferredoxin PetF (ssl0020), the presumed natural electron acceptor/donor to ADO in Synechocystis sp. PCC 6803, was over-expressed as part of the engineered pathways. Consequently, co-expression of PetF improved propane production by roughly two-fold for both the atoB-TPC7 and nphT7-TPC7 pathway in combination with both ADO and ADOA134F (
Removing Competing Pathways for Propane Synthesis
E. coli cells contain a wide range of aldehyde reductases and alcohol dehydrogenases which act to scavenge potentially toxic intracellular aldehydes [Rodriguez G M, Atsumi S: Metab Eng 2014, 25:227-237]. Two of the native aldehyde reductases in E. coli, Ahr and YqhD, were previously shown to compete for butyraldehyde [Kallio P et al. Nature communications 2014, 5:4731]. In order to further optimize propane production, Δahr and ΔyqhD single and double knockout E. coli strains were therefore tested (
Potential for Large-scale Production of Propane
As propane biosynthesis was only analysed in small-scale cultures (2.0 mL GC vials), larger scale productivity and longevity of propane production was also investigated with the best performing strains (i.e. atoB-TPC7 or nphT7-TPC7 routes incorporated into E. coli ΔyqhD in the presence of the ADOA134F variant and ferredoxin). The culture volume was scaled 400-fold to 200 mL, in 300 mL flasks sealed with air-tight rubber septa, and propane production and accumulated residual butanol were monitored over 12 hours (
Recent progress in exploring various heterologous microbial platforms towards metabolites such as fatty acids, alcohols and alkanes has improved the prospect of advanced biofuel production. Propane (C3H8) was proposed as a new microbial biofuel target as it would be able to act as a direct drop-in replacement for the corresponding non-renewable products currently in use (e.g. autogas, LPG). Propane also has very good physicochemical properties which allow it to be stored and transported in a compressed liquid form, while under ambient conditions it is a clean-burning gas used in various applications ranging from heating to utilization as a transport fuel. The very first microbial biosynthetic pathway for producing propane was recently engineered in E. coli [Kallio P et al. Nature communications 2014, 5:4731]. In that study, it was found that the biosynthesis of propane was restricted by the poor activity of the enzyme ADO towards short-chain substrates in combination with competing pathways that limits the availability of the ADO substrate, butyraldehyde. Herein, the inventors therefore explored alternative biosynthetic approaches to provide a more comprehensive understanding of the limiting factors in microbial propane biosynthesis, and to find possible ways to overcome metabolic bottlenecks.
The pathways assembled in the present study are not dependent on type II fatty acid biosynthesis unlike in the previous study, and instead use CoA—rather than ACP-based intermediates. Importantly, this strategy allowed us to bypass the strict regulatory control on native fatty acid biosynthesis flux [Kallio P et al. Nature communications 2014, 5:4731; Davis MS, Cronan J E J Bacteriol 2001, 183:1499-1503; James E S, Cronan J E J Biol Chem 2004, 279:2520-2527]. The pathways engineered herein comprise four parallel variations of the Clostridial butanol pathway, differing from one another in the conversion of (1) acetyl-CoA to acetoacetyl-CoA (atoB vs. nphT7 routes) and (2) butyryl-CoA to butyraldehyde (adhE2 vs, TPC7 routes). In the first stage of pathway evaluation, production of butanol was used as a measure of metabolic flux towards butyraldehyde, the immediate substrate for propane production by ADO. The TPC7 variant routes were developed in order to avoid the bifunctional activity of AdhE2 which includes the reduction of butyraldehyde to 1-butanol. Such a reaction would be expected to compete with ADO for the butyraldehyde intermediate and lower propane production. Therefore, in order to separate these functional activities and allow greater pathway flexibility, AdhE2 was replaced with a ‘TPC7’ module in combination with Ahr.
For the first two variant pathways, the (co)substrate requirements for AtoB and NphT7 are quite distinct; AtoB requires two molecules of acetyl CoA whereas nphT7 requires acetyl-CoA along with malonyl-CoA. Both reactions result in the release of CoA though in the latter case there is also release of CO2. The greater 1-butanol levels observed with the AtoB pathway suggest that it is a preferred route for acetoacetyl CoA production in E. coli. However it is also important to note that AtoB has a higher protein expression level in E. coli compared to nphT7 (
Quantitative comparisons revealed a difference of almost three orders of magnitude between the titres of butanol and propane (173 mg/L vs 0.2 mg/L) in the wild-type strains of AtoB-TPC7-Ahr and AtoB-TPC7-ADO. This difference can be attributed partly to the poor efficiency of the last biosynthetic step (conversion of butyraldehyde to propane). This is consistent with the earlier reports that have demonstrated in vitro and in vivo in E. coli that ADO has very low activity even towards the most preferred native substrates (C10-C15), and especially towards C4 substrates. To partially alleviate this constraint, the ADOA143F variant was introduced into the engineered pathways to replace native ADO. This resulted in a 1.8 fold improvement in propane, most likely reflecting more efficient utilization of the intracellular pool of available butyraldehyde. Furthermore, as confirmed herein, the reduction of ADO in the presence of a 2Fe-2S ferredoxin improves the metabolic reactions by mediating electron transfer in partner enzymes as observed herein.
The inventors' work introduces an alternative and conceptually different pathway for propane production in comparison to the previous work [Kallio P et al. Nature communications 2014, 5:4731] without the disadvantage of reliance on the FAS pathway, and which is appropriate for viable yet renewable mass-production of this key industrial chemical.
The various methods and techniques described above provide a number of ways to carry out the application. Of course, it is to be understood that not necessarily all objectives or advantages described can be achieved in accordance with any particular embodiment described herein. Thus, for example, those skilled in the art will recognize that the methods can be performed in a manner that achieves or optimizes one advantage or group of advantages as taught herein without necessarily achieving other objectives or advantages as taught or suggested herein. A variety of alternatives are mentioned herein. It is to be understood that some preferred embodiments specifically include one, another, or several features, while others specifically exclude one, another, or several features, while still others mitigate a particular feature by inclusion of one, another, or several advantageous features.
Furthermore, the skilled artisan will recognize the applicability of various features from different embodiments. Similarly, the various elements, features and steps discussed above, as well as other known equivalents for each such element, feature or step, can be employed in various combinations by one of ordinary skill in this art to perform methods in accordance with the principles described herein. Among the various elements, features, and steps some will be specifically included and others specifically excluded in diverse embodiments.
Although the application has been disclosed in the context of certain embodiments and examples, it will be understood by those skilled in the art that the embodiments of the application extend beyond the specifically disclosed embodiments to other alternative embodiments and/or uses and modifications and equivalents thereof.
Preferred embodiments of this application are described herein, including the best mode known to the inventors for carrying out the application. Variations on those preferred embodiments will become apparent to those of ordinary skill in the art upon reading the foregoing description. It is contemplated that skilled artisans can employ such variations as appropriate, and the application can be practiced otherwise than specifically described herein. Accordingly, many embodiments of this application include all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the application unless otherwise indicated herein or otherwise clearly contradicted by context.
All patents, patent applications, publications of patent applications, and other material, such as articles, books, specifications, publications, documents, things, and/or the like, referenced herein are hereby incorporated herein by this reference in their entirety for all purposes, excepting any prosecution file history associated with same, any of same that is inconsistent with or in conflict with the present document, or any of same that may have a limiting affect as to the broadest scope of the claims now or later associated with the present document. By way of example, should there be any inconsistency or conflict between the description, definition, and/or the use of a term associated with any of the incorporated material and that associated with the present document, the description, definition, and/or the use of the term in the present document shall prevail.
It is to be understood that the embodiments of the application disclosed herein are illustrative of the principles of the embodiments of the application. Other modifications that can be employed can be within the scope of the application. Thus, by way of example, but not of limitation, alternative configurations of the embodiments of the application can be utilized in accordance with the teachings herein. Accordingly, embodiments of the present application are not limited to that precisely as shown and described.
Various embodiments of the invention are described above in the Detailed Description. While these descriptions directly describe the above embodiments, it is understood that those skilled in the art may conceive modifications and/or variations to the specific embodiments shown and described herein. Any such modifications or variations that fall within the purview of this description are intended to be included therein as well. Unless specifically noted, it is the intention of the inventors that the words and phrases in the specification and claims be given the ordinary and accustomed meanings to those of ordinary skill in the applicable art(s).
The foregoing description of various embodiments of the invention known to the applicant at this time of filing the application has been presented and is intended for the purposes of illustration and description. The present description is not intended to be exhaustive nor limit the invention to the precise form disclosed and many modifications and variations are possible in the light of the above teachings. The embodiments described serve to explain the principles of the invention and its practical application and to enable others skilled in the art to utilize the invention in various embodiments and with various modifications as are suited to the particular use contemplated. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed for carrying out the invention.
While particular embodiments of the present invention have been shown and described, it will be obvious to those skilled in the art that, based upon the teachings herein, changes and modifications may be made without departing from this invention and its broader aspects and, therefore, the appended claims are to encompass within their scope all such changes and modifications as are within the true spirit and scope of this invention.
This application claims the benefit of co-pending U.S. Provisional Patent Application No. 62/289,517, filed 1 Feb. 2016, which is hereby incorporated herein.
| Number | Name | Date | Kind |
|---|---|---|---|
| 5585089 | Queen et al. | Dec 1996 | A |
| 5721109 | Yano et al. | Feb 1998 | A |
| 20070054335 | Esfandiari et al. | Mar 2007 | A1 |
| Number | Date | Country |
|---|---|---|
| H07248329 | Sep 1995 | JP |
| 9839025 | Sep 1998 | WO |
| 2005116654 | Dec 2005 | WO |
| 2010086667 | Aug 2010 | WO |
| 2012131394 | Oct 2012 | WO |
| 2013186679 | Dec 2013 | WO |
| Entry |
|---|
| GB Search Report for GB Application No. GB1514411.6 dated May 26, 2017, 4 pages. |
| Kallio et al., “An engineered pathway for the biosynthesis of renewable propane,” Nature Communications, dated Sep. 2, 2014, 8 pages. |
| Menon et al., “A microbial platform for renewable propane synthesis based on a fermentative butanol pathway,” Biotechnology for Biofuels, (2015), 8:61, 12 pages. |
| Akhtar et al., “Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities,” PNAS, dated Jan. 2, 2013, vol. 110, No. 1, pp. 87-92. |
| Atsumi S. et al., “Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde,” Nat Biotechnol., Dec. 2009, 27(12):1177-1180. Abstract provided. |
| Bond-Watts BB et al., “Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways,” Nat Chem Biol., Apr. 2011, 7(4):222-227. Abstract provided. |
| Davis et al., “Inhibition of Escherichia coli Aceytl Coenzyme A Carboxylase by Acyl-Acyl Carrier Protein,” Journal of Bacteriology, Feb. 2001, vol. 183, No. 4, 1499-1503. |
| Dellomonaco C. et al., “Engineered reversal of the β-oxidation cycle for the synthesis of fuels and checmicals,” Nature, Aug. 10, 2011, 476(7360):355-359. Abstract provided. |
| Howard et al., “Sythesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli,” PNAS, May 7, 2013, vol. 110, No. 19, 7636-7641. |
| James et al., “Expression of Two Escherichia coli Acetyl-CoA Carboxylase Subunits Is Autoregulated,” The Journal of Biological Chemistry, (2004), vol. 279, No. 4, 2520-2527. |
| Köhler et al., “Deruvation of specific antibody-producing tissue culture and tumor lines by cell fusion,” Eur J Immunol., Jul. 6, 1976, (7):511-519. Abstract Provided. |
| Lan et al., “ATP drives direct photosynthetic production of 1-butanol in cyanobacteria,” PNAS, Apr. 17, 2012, vol. 109, No. 16, 6018-6023. |
| Pásztor et al., “A Synthetic O2-Tolerant Butanol Pathway Exploiting Native Fatty Acid Biosynthesis in Escherichia coli,” Biotechnol. Bioeng., 2015; 112: 120-128. |
| Riechmann et al., “Reshaping human antibodies for therapy,” Nature, Mar. 24, 1988, 332(6162): 323-327. Abstract provided. |
| Rodriguez et al., “Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli,” Metab Eng., Sep. 2014, 25: 227-237. |
| Schirmer et al., “Microbial biosynthesis of alkanes,” Science, Jul. 30, 2010, 329(5991): 559-562. Abstract provided. |
| El et al., “Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide,” Metab. Eng., Jul. 2011, 13(4): 353-363, Abstract Provided. |
| Lan et al., “Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria,” Energy Environ. Sci, (2013), 6, 2672-2681. Abstract provided. |
| International Preliminary Report on Patentability for corresponding PCT Application No. PCT/GB2015/052340, dated Feb. 23, 2017, 6 pages. |
| Number | Date | Country | |
|---|---|---|---|
| 20170218401 A1 | Aug 2017 | US |
| Number | Date | Country | |
|---|---|---|---|
| 62289517 | Feb 2016 | US |
