Claims
- 1. A composition for selectively binding a desired nucleotide sequence, said composition comprising from 2 to 4 palladium(II)-bound ligands, said palladium(II)-bound ligands being tethered together by an organic backbone having from 3 to 20 atoms between each palladium(II)-bound ligand, thereby forming a palladium(II)-bound ligand backbone grouping, said palladium(II)-bound ligand backbone grouping being covalently bonded to a solid support, and wherein each palladium(II)-bound ligand is independently selective of a desired heterocyclic aromatic amine base of the nucleotide sequence to be selectively bound.
- 2. A composition as in claim 1, comprising 2 palladium(II)-bound ligands.
- 3. A composition as in claim 1, comprising 3 palladium(II)-bound ligands.
- 4. A composition as in claim 1, comprising 4 palladium(II)-bound ligands.
- 5. A composition as in claim 1 being configured to select a nucleotide sequence having predetermined adjacent heterocyclic aromatic amine bases.
- 6. A composition as in claim 1, wherein each palladium(II)-bound ligand is independently selective of the same heterocyclic aromatic amine base of the nucleotide sequence.
- 7. A composition as in claim 1, wherein each palladium(II)-bound ligand is independently selective of different heterocyclic aromatic amine base of the nucleotide sequence.
- 8. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands comprises the sequence
- 9. A composition as in claim 1 wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands comprises the sequence
- 10. A composition as in claim 9, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selected from the group consisting of dipicolinic acid and aminodiphosphate.
- 11. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands comprises the sequence
- 12. A composition as in claim 11, wherein the at least one ligand is
- 13. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands comprises the sequence
- 14. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selective of adenine (A).
- 15. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selective of guanine (G).
- 16. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selective of thymine (T).
- 17. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selective of uracil (U).
- 18. A composition as in claim 1, wherein at least one ligand of the 2 to 4 palladium(II)-bound ligands is selective of cytosine (C).
- 19. A composition as in claim 1, wherein said composition is configured for use in selectively binding DNA sequences.
- 20. A composition as in claim 1, wherein said composition is configured for use in selectively binding RNA sequences.
- 21. A composition as in claim 1, wherein the solid support is an inorganic solid support and the palladium(II)-bound ligand backbone grouping is bonded to the solid support through a silane covalent linkage mechanism and an organic spacer grouping having from 1 to 10 atoms.
- 22. A composition as in claim 1, wherein the solid support is an organic solid support and the palladium(II)-bound ligand backbone grouping is bonded to the solid support through an organic covalent linkage mechanism having from 1 to 10 atoms.
- 23. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support, said palladium(II)-bound ligand being complexed to a heterocyclic aromatic amine base.
- 24. A composition as in claim 23, wherein the heterocyclic aromatic amine base is in the form of a nucleoside.
- 25. A composition as in claim 23, wherein the heterocyclic aromatic amine base is in the form of a nucleotide.
- 26. A composition as in claim 23, wherein the heterocyclic aromatic amine base comprises adenine (A).
- 27. A composition as in claim 23, wherein the heterocyclic aromatic amine base c omprises thymine (T).
- 28. A composition as in claim 23, wherein the heterocyclic aromatic amine base comprises uracil (U).
- 29. A composition as in claim 23, wherein the heterocyclic aromatic amine base comprises guanine (G).
- 30. A composition as in claim 23, wherein the heterocyclic aromatic amine base comprises cytosine (C).
- 31. A composition according to claim 29, wherein the ligand of the palladium(II)-bound ligand comprises the sequence
- 32. A composition according to claim 31, wherein x is 2 and y is 1.
- 33. A composition according to claim 32, wherein B is nitrogen.
- 34. A composition according to claim 26 wherein the ligand of the palladium(II)-bound ligand comprises the sequence
- 35. A composition according to claim 34, the ligand is selected from the group consisting of dipicolinic acid and aminodiphosphate.
- 36. A composition according to claim 27, wherein the ligand of the palladium(II)-bound ligand comprises the sequence
- 37. A composition according to claim 28, wherein the ligand of the palladium(II)-bound ligand comprises the sequence
- 38. A composition according to claim 36, wherein the ligand is
- 39. A composition according to claim 37, wherein the ligand is
- 40. A composition according to claim 30, wherein the ligand of the palladium(II)-bound ligand comprises the sequence
- 41. A composition as in claim 23, wherein the solid support is an inorganic solid support and the palladium(II)-bound ligand backbone grouping is bonded to the solid support through a silane covalent linkage mechanism and an organic spacer grouping.
- 42. A composition as in claim 23, wherein the solid support is an organic solid support and the palladium(II)-bound ligand backbone grouping is bonded to the solid support through an organic covalent linkage mechanism having from 1 to 10 atoms.
- 43. A method for concentrating, removing, or separating a desired heterocyclic aromatic amine base or a nucleotide sequence containing said desired heterocyclic aromatic amine base from a source solution comprising the steps of:
(a) contacting a source solution having a first volume with a composition comprising a palladium(II)-bound ligand covalently bonded to a solid support, said source solution containing a desired heterocyclic aromatic amine base and at least one undesired heterocyclic aromatic amine base, wherein the palladium(II)-bound ligand is preferentially selective of the desired heterocyclic aromatic amine base; (b) removing the source solution from contact with said composition to which said desired heterocyclic aromatic amine base has been complexed; (c) contacting said composition having said desired heterocyclic aromatic amine bases associated therewith with a smaller volume of an aqueous receiving solution in which said desired heterocyclic aromatic amine bases are stripped from the composition; and (d) recovering said desired heterocyclic aromatic amine bases in concentrated form in said receiving solution.
- 44. A method as in claim 43 wherein said desired heterocyclic aromatic amine base is guanine (G), and the ligand portion of the palladium(II)-bound ligand comprises the sequence
- 45. A method as in claim 43 wherein said desired heterocyclic aromatic amine base is adenine (A), and the ligand portion of the palladium(II)-bound ligand comprises the sequence
- 46. A method as in claim 43, wherein said desired heterocyclic aromatic amine base is thymine (T), and the ligand portion of the palladium(II)-bound ligand comprises the sequence
- 47. A method as in claim 43, wherein said desired heterocyclic aromatic amine base is uracil (U), and the ligand portion of the palladium(II)-bound ligand comprises the sequence
- 48. A method as in claim 43, wherein said desired heterocyclic aromatic amine base is cytosine (C), and the ligand portion of the palladium(II)-bound ligand comprises the sequence
- 49. A method as in claim 43, wherein the aqueous receiving solution is desired from the group consisting of ammonia, ammonia buffer, ethylene diamine, and thiocyanate.
- 50. A method as in claim 49, wherein the aqueous receiving solution is ammonia or ammonia buffer.
- 51. A method as in claim 43, wherein the aqueous receiving solution is strong enough to displace the heterocyclic aromatic amine base from the composition without altering its configuration and is not strong enough to displace the Pd(II) from the c omposition.
- 52. A method as in claim 43, further comprising a washing step to remove the aqueous receiving solution from the composition for further use.
- 53. A method as in claim 43, wherein the composition is configured for separating individual heterocyclic aromatic amine bases.
- 54. A method as in claim 43, wherein the composition is configured for separating heterocyclic aromatic amine bases that are part of nucleotide sequences.
- 55. A method for concentrating, removing, or separating a nucleotide chain containing a specific nucleotide sequence from a source solution comprising the steps of:
(a) identifying a nucleotide chain that contains a desired nucleotide sequence that is desired for concentrating, removing, or separating from a source solution; (b) providing a composition that will selectively bind the nucleotide sequence; (c) contacting the source solution having a first volume with the composition wherein a chemical attraction between the composition and the nucleotide sequence occurs; (d) removing the source solution from contact with the composition, said composition having the nucleotide sequence attracted thereto; (e) contacting the composition having the nucleotide sequence attracted thereto with a smaller volume of an aqueous receiving solution in which said nucleotide chain is stripped from the composition; and (f) recovering the nucleotide chain in concentrated form in the receiving solution.
- 56. A method as in claim 55, wherein the nucleotide sequence contains from 2 to 4 heterocyclic amine bases.
- 57. A method as in claim 55, wherein the source solution contains other non-desired nucleotide sequences are present in the source solution
- 58. A method as in claim 55, wherein the composition has from 2 to 4 palladiumr(II)-bound ligands, said palladium(II)-bound ligands being tethered together by an organic backbone having from 3 to 20 atoms between each palladium(II)-bound ligand, thereby forming a palladium(II)-bound ligand backbone grouping, said palladium(II)-bound ligand backbone grouping covalently bonded to a solid support, wherein each palladium(II)-bound ligand is independently selective of a desired heterocyclic aromatic amine base of the nucleotide sequence.
- 59. A method as in claim 55, wherein the nucleotide chain is the same as the nucleotide sequence.
- 60. A method as in claim 55, wherein the nucleotide chain is longer than the nucleotide sequence.
- 61. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support through a spacer, said ligand portion of the palladium(II)-bound ligand comprising the sequence
- 62. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support through a spacer, said ligand portion of the palladium(II)-bound ligand comprising the sequence
- 63. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support through a spacer, said ligand portion of the palladium(II)-bound ligand comprising the sequence
- 64. A composition as in claim 63, wherein the ligand is
- 65. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support through a spacer, said ligand portion of the palladium(II)-bound ligand comprising the sequence
- 66. A composition as in claim 65, wherein the ligand is
- 67. A composition, comprising a palladium(II)-bound ligand covalently bonded to a solid support through a spacer, said ligand portion of the palladium(II)-bound ligand comprising the sequence
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/290,577 filed on May 11, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60290577 |
May 2001 |
US |