Claims
- 1. An isolated nucleic acid sequence encoding carbamoyl phosphate synthase of T. gondii.
- 2. The nucleic acid sequence of claim 1 comprising SEQ ID NO:1 or SEQ ID NO:2.
- 3. A pyrimidine auxotroph mutant of an apicomplexan wherein one of six enzymes of the de novo pyrimidine biosynthesis pathway of apicomplexans is mutated.
- 4. The pyrimidine auxotroph mutant of claim 3 wherein the apicomplexan is T. gondii.
- 5. A method for protecting an animal against infection by an apicomplexan comprising administering to an animal a pyrimidine auxotroph mutant of claim 3.
- 6. The method of claim 5 wherein the apicomplexan is T. gondii.
- 7. A method for screening for inhibitors of pyrimidine salvage enzymes in apicomplexans comprising: contacting a pyrimidine auxotroph mutant of claim 3 with a compound suspected of being an inhibitor of a pyrimidine salvage enzyme; and determining growth of the pyrimidine auxotroph mutant in the presence of the compound, wherein inhibition of growth of the mutant is indicative of the compound being an inhibitor of a pyrimidine salvage enzyme.
- 8. A vaccine for protection against infection by an apicomplexan comprising the pyrimidine auxotroph mutant of claim 3 and a pharmaceutically acceptable carrier or diluent.
INTRODUCTION
[0001] This application is a continuation-in-part of PCT/US01/03906, filed Feb. 7, 2001, which claims the benefit of priority of U.S. Provisional Application No. 60/180,604, filed Feb. 7, 2000, now abandoned.
Government Interests
[0002] This invention was supported in part by funds from the U.S. government (NIH Grant No. R01 AI41930) and the U.S. government may therefore have certain rights in the invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60180604 |
Feb 2000 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
PCT/US01/03906 |
Feb 2001 |
US |
Child |
10094679 |
Mar 2002 |
US |