Claims
- 1. A method of isolating DNA from a biological sample comprising:
selectively binding DNA to a solid phase by contacting the biological sample with the solid phase under conditions which selectively bind DNA; separating the solid phase with the bound DNA from an unbound portion of the biological sample; and isolating the DNA from the solid phase.
- 2. The method of claim 1, wherein the isolating the DNA from the solid phase comprises eluting the DNA.
- 3. The method of claim 1, wherein the solid phase is a siliceous material.
- 4. The method of claim 3, wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
- 5. The method of claim 4, wherein the solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
- 6. The method of claim 1, wherein the conditions which selectively bind DNA comprise using a binding buffer comprising:
an alkaline pH; and a large anion, wherein the large anion is at least as large as a bromide ion.
- 7. The method of claim 6, wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchlorate, and sulfosalicylate.
- 8. The method of claim 6, wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
- 9. The method of claim 6, wherein the large anion is selected from at least one of iodide and bromide.
- 10. The method of claim 6, wherein the alkaline pH is equal to, or above 8.0.
- 11. The method of claim 6, wherein the alkaline pH is equal to, or above 9.0.
- 12. The method of claim 6, wherein the alkaline pH is equal to, or above 10.0.
- 13. A method for isolating DNA and RNA from a biological sample comprising:
selectively binding DNA to a first solid phase by contacting the biological sample with the first solid phase under conditions which selectively bind DNA; separating the first solid phase with the bound DNA from a first unbound portion of the biological sample; isolating the DNA from the first solid phase; and isolating RNA from the first unbound portion of the biological sample.
- 14. The method of claim 13, wherein the isolating the DNA from the first solid phase comprises eluting the DNA.
- 15. The method of claim 13, wherein the isolating of the RNA from the first unbound portion of the biological sample comprises:
exposing the first unbound portion of the biological sample to a second solid phase under conditions which bind RNA to the second solid phase; separating the second solid phase with bound RNA from a second unbound portion of the biological sample; and isolating the RNA from the second solid phase by eluting the RNA.
- 16. The method of claim 15, wherein the conditions which bind RNA to the second solid phase comprise a neutral or acidic pH.
- 17. The method of claim 15, wherein the second solid phase is a siliceous material.
- 18. The method of claim 17, wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
- 19. The method of claim 15, wherein the second solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
- 20. The method of claim 15, wherein the conditions which selectively bind DNA comprise using a binding buffer comprising:
an alkaline pH; and a large anion, wherein the large anion is at least as large as a bromide ion.
- 21. The method of claim 20, wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchlorate, and sulfosalicylate.
- 22. The method of claim 20, wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
- 23. The method of claim 20, wherein the large anion is selected from at least one of iodide and bromide.
- 24. The method of claim 20, wherein the alkaline pH is equal to, or above 8.0.
- 25. The method of claim 20, wherein the alkaline pH is equal to, or above 9.0.
- 26. The method of claim 20, wherein the alkaline pH is equal to, or above 10.0.
- 27. The method of claim 21, wherein the conditions which bind RNA to the second solid phase comprise a neutral or acidic pH.
- 28. A method of isolating nucleic acid from a biological sample comprising:
binding nucleic acid to a first solid phase by contacting the biological sample with the first solid phase under conditions which bind both RNA and DNA; separating the first solid phase with bound nucleic acid from a first unbound portion of the biological sample; eluting RNA from the first solid phase with bound nucleic acid under conditions which selectively bind DNA; removing the first solid phase with bound DNA from a first eluate; and isolating the DNA from the first solid phase.
- 29. The method of claim 28, wherein the isolating the DNA from the first solid phase comprises eluting the DNA from the first solid phase.
- 30. The method of claim 28, wherein the first solid phase is a siliceous material.
- 31. The method of claim 30, wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
- 32. The method of claim 31, wherein the first solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
- 33. The method of claim 28, wherein the conditions which selectively bind DNA comprise using a binding buffer comprising:
an alkaline pH; and a large anion, wherein the large anion is at least as large as a bromide ion.
- 34. The method of claim 33, wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchlorate, and sulfosalicylate.
- 35. The method of claim 33, wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
- 36. The method of claim 33, wherein the large anion is selected from at least one of iodide and bromide.
- 37. The method of claim 33, wherein the alkaline pH is equal to, or above 8.0.
- 38. The method of claim 33, wherein the alkaline pH is equal to, or above 9.0.
- 39. The method of claim 33, wherein the alkaline pH is equal to, or above 10.0.
- 40. The method of claim 28, further comprising:
exposing the first eluate to a second solid phase under conditions which bind RNA to the second solid phase; separating the second solid phase with the bound RNA from an unbound portion of the first eluate; and isolating the RNA from the second solid phase.
- 41. The method of claim 40, wherein the isolating the RNA from the second solid phase comprises eluting the RNA.
- 42. The method of claim 40, wherein the second solid phase is a siliceous material.
- 43. The method of claim 42, wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
- 44. The method of claim 43, wherein the second solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
- 45. The method of claim 40, wherein the conditions which bind RNA to the second solid phase comprise a neutral or acidic pH.
- 46. The method of claim 40, wherein the conditions which selectively bind DNA comprise using a binding buffer comprising:
an alkaline pH; and a large anion, wherein the large anion is at least as large as a bromide ion.
- 47. The method of claim 46, wherein the conditions which bind RNA to the second solid phase comprise a neutral or acidic pH.
- 48. A method of identifying DNA in a biological sample comprising:
selectively binding DNA to a solid phase by contacting the biological sample with the solid phase under conditions which selectively bind DNA; separating the solid phase with the bound DNA from an unbound portion of the biological sample; and identifying the DNA bound to the solid phase.
- 49. The method of claim 48, wherein the identifying the DNA bound to the solid phase comprises amplifying the DNA bound to the solid phase.
- 50. A method for identifying DNA and RNA in a biological sample comprising:
selectively binding DNA to a first solid phase by contacting the biological sample with the first solid phase under conditions which selectively bind DNA; separating the first solid phase with the bound DNA from a first unbound portion of the biological sample; identifying the DNA bound to the first solid phase; and identifying the RNA from the first unbound portion of the biological sample.
- 51. The method of claim 50, wherein the identifying the DNA bound to the first solid phase comprises amplifying the DNA bound to the first solid phase.
- 52. A method of identifying nucleic acid in a biological sample comprising:
binding nucleic acid to a first solid phase by contacting the biological sample with the first solid phase under conditions which bind both DNA and RNA; separating the first solid phase with bound nucleic acid from a first unbound portion of the biological sample; eluting RNA from the first solid phase with bound nucleic acid under conditions which selectively bind DNA; removing the first solid phase with bound DNA from a first eluate; and identifying the DNA bound to the first solid phase.
- 53. The method claim 52, wherein the identifying the DNA bound to the first solid phase comprises amplifying the DNA bound to the first solid phase.
- 54. A kit comprising:
a buffer with an alkaline pH; a large anion, wherein the large anion is at least as large as a bromide ion; and a solid phase.
- 55. The kit of claim 54, wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchlorate, and sulfosalicylate.
- 56. The kit of claim 54, wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
- 57. The kit of claim 54, wherein the large anion is selected from at least one of iodide and bromide.
- 58. The kit of claim 54, wherein the alkaline pH is equal to, or above 8.0.
- 59. The kit of claim 54, wherein the alkaline pH is equal to, or above 9.0.
- 60. The kit of claim 54, wherein the alkaline pH is equal to, or above 10.0.
- 61. A kit comprising:
a solid phase; a nucleic acid binding buffer, wherein both DNA and RNA bind the solid phase under conditions generated by the nucleic acid binding buffer; and a selective DNA binding buffer, wherein the conditions generated by the selective DNA binding buffer allow selective binding of DNA to the solid phase.
- 62. The kit of claim 61, further comprising an RNA binding buffer, wherein the conditions generated by the RNA binding buffer allow RNA to bind to a solid phase.
PRIORITY DATA
[0001] This application claims priority benefit of U.S. Provisional Application Serial No. 60/334,029, filed Nov. 28, 2001. Application No. 60/334,029 is incorporated by reference herein in its entirety for any purpose.
Provisional Applications (1)
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Number |
Date |
Country |
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60334029 |
Nov 2001 |
US |