Claims
- 1. An isolated polynucleotide comprising a sequence selected from the group consisting of: SEQ ID NO: 1-44.
- 2. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) complements of SEQ ID NO: 1-44; (b) reverse complements of SEQ ID NO: 1-44; (c) reverse sequences of SEQ ID NO: 1-44; (d) sequences that are 100-mers of a sequence of SEQ ID NO: 1-44; (e) sequences that are 40-mers of a sequence of SEQ ID NO: 1-44; and (f) sequences that are 20-mers of a sequence of SEQ ID NO: 1-44.
- 3. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) sequences having at least 75% identity to a sequence of SEQ ID NO: 1-44; (b) sequences having at least 90% identity to a sequence of SEQ ID NO: 1-44; (c) sequences having at least 95% identity to a sequence of SEQ ID NO: 1-44; (d) sequences having at least 98% identity to a sequence of SEQ ID NO: 1-44; and (e) sequences that hybridize to a sequence of SEQ ID NO: 1-44 under stringent hybridization conditions, wherein the polynucleotide encodes a polypeptide having substantially the same functional activity as a polypeptide encoded by a polynucleotide of SEQ ID NO: 1-44.
- 4. An isolated polypeptide encoded by a polynucleotide of any one of claims 1-3.
- 5. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: sequences recited in SEQ ID NO: 45-88.
- 6. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) sequences having at least 75% identity to a sequence of SEQ ID NO: 45-88; (b) sequences having at least 90% identity to a sequence of SEQ ID NO: 45-88; and (c) sequences having at least 95% identity to a sequence of SEQ ID NO: 45-88, wherein the polypeptide has substantially the same functional properties as a polypeptide of SEQ ID NO: 45-88.
- 7. An isolated polynucleotide that encodes a polypeptide of any one of claims 5 and 6.
- 8. An isolated oligonucleotide probe or primer comprising at least 10 contiguous residues complementary to 10 contiguous residues of a nucleotide sequence recited in any one of claims 1-3.
- 9. A kit comprising a plurality of oligonucleotide probes or primers of claim 8.
- 10. A genetic construct comprising a polynucleotide of any one of claims 1-3.
- 11. A transgenic cell comprising a genetic construct according to claim 10.
- 12. A genetic construct comprising, in the 5′-3′ direction:
(a) a gene promoter sequence; (b) a polynucleotide sequence comprising at least one of the following: (1) a polynucleotide coding for at least a functional portion of a polypeptide of any one of claims 5 and 6; and (2) a polynucleotide comprising a non-coding region of a polynucleotide of any one of claims 1-3; and (c) a gene termination sequence.
- 13. The genetic construct of claim 12, wherein the polynucleotide sequence is in a sense orientation.
- 14. The genetic construct of claim 12, wherein the polynucleotide is in an anti-sense orientation.
- 15. A transgenic plant cell comprising a genetic construct of claim 12.
- 16. A plant comprising a transgenic plant cell according to claim 12, or fruit or seeds or progeny thereof.
- 17. The plant of claim 16, wherein the plant is selected from the group consisting of Festuca arundinacea and Lolium perenne species.
- 18. A method for modulating at least one of the fructan composition, cellulose composition, starch composition and tannin composition of a plant, comprising modulating the activity of a polypeptide of any one of claims 5 and 6 in the plant.
- 19. A method for modulating at least one of the fructan composition, cellulose composition, starch composition and tannin composition of a plant, comprising modulating the activity of a polynucleotide of any one of claims 1-3 in the plant.
- 20. The method of claim 19, comprising stably incorporating into the genome of the plant a polynucleotide of any one of claims 1-3.
- 21. The method of claim 19, comprising stably incorporating into the genome of the plant a genetic construct of claim 12.
- 22. A method for producing a plant having at least one of altered fructan composition, altered cellulose composition, altered starch composition and altered tannin composition, comprising:
(a) transforming a plant cell with a genetic construct of claim 12 to provide a transgenic cell; and (b) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
- 23. A method for modifying the activity of a polypeptide involved in a fructan, cellulose, starch or tannin biosynthetic pathway in a plant, comprising modulating the activity of a polynucleotide of any one of claims 1-3 in the plant.
- 24. The method of claim 23, comprising stably incorporating into the genome of the plant a genetic construct of claim 12.
- 25. A method for modifying the activity of a polypeptide involved in a fructan, cellulose, starch or tannin biosynthetic pathway in a plant, comprising introducing into cells of the plant double stranded RNA corresponding to a polynucleotide of any one of claims 1-3, thereby inhibiting expression of a polypeptide encoded by the polynucleotide.
Parent Case Info
[0001] CROSS-REFERENCE TO RELATED APPLICATIONS
[0002] This application claims priority to U.S. Provisional Patent Application 60/378,930, filed May 6, 2002, and U.S. Provisional Patent Application 60/408,782, filed Sep. 5, 2002.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60378930 |
May 2002 |
US |
|
60408782 |
Sep 2002 |
US |