Compositions isolated from plant cells and their use in the modification

Abstract

Novel isolated polynucleotides that encode polypeptides involved in plant cell signaling are provided, together with DNA constructs comprising such polynucleotides. Methods for using such constructs for the modulation of cell signaling in plants are also disclosed, together with transgenic plants comprising such constructs.

Description

FIELD OF THE INVENTION

This invention relates to the field of modifying the responses of plant cells to external signals, such as environmental changes, and developmental cues. More specifically, this invention provides isolated polynucleotides encoding polypeptides that are integrally located in plant cell membranes and that mediate cellular signaling processes.

BACKGROUND OF THE INVENTION

Plants progress through set developmental programs throughout the course of their lifetimes. This is particularly evident in embryogenesis and floral development. There are a variety of signal molecules produced by certain cells in the plant to which other cells, particularly in the meristematic regions, are poised to respond. These signal molecules trigger distinct sets of developmental programs at specific times that lead to the formation of, for example, flowers or cotyledons. In addition to the programmed developmental pathways, plants are exposed to a variety of environmental stimuli such as changes in temperature and amount of sunlight, availability of water, wounding from mechanical injury and attack by pathogens. Environmental factors, such as exposure to light, heat, cold, drought, etc., activate the expression of genes and synthesis of proteins and other compounds essential for an appropriate response to the environmental signal and thereby, the healthy development of the plant. These responses, like the developmental pathways, are mediated by signal molecules.

To respond to these signal molecules, plant cells produce surface receptor proteins that serve as sensors, regulators and/or transducers of cell signals. The intracellular transduction of a signal is often transmitted via a phosphorylation cascade of molecules that culminates in the transcription of genes to elicit the appropriate cellular response either for normal development or against environmental challenge.

One major class of receptor proteins is the single-transmembrane family, of which there are several subclasses. These proteins are characterized by three domains: an extracellular signal molecule (or ligand) recognition/binding domain, a single cell membrane-spanning domain and an intracellular signal transduction domain which is usually a protein kinase. Many, but not all, plant single transmembrane proteins belong to the subclass known as receptor-like kinases (RLKs). The intracellular kinase domains of plant RLKs are all serine/threonine protein kinases, while the extracellular domains of RLKs are of different types. One type of RLK is characterized by the presence of the extracellular S-domain, originally described in self-incompatibility-locus glycoproteins that inhibit self-pollination. The S-domain is recognized by an array of ten cysteine residues in combination with other conserved residues. Another class of RLKs has an extracellular domain distinguished by leucine rich repeats (LRR) that are involved in protein-protein interactions. Binding of ligands to the extracellular domain is followed by receptor dimerization, autophosphorylation and the activation of a series of intracellular proteins which serve to transduce the signal to the nucleus. The structure of plant RLKs is very similar to receptors found in cell signaling pathways in animal systems.

One example of a plant RLK is the Xa21 gene, which confers resistance to the plant pathogen Xanthomonas oryzae pv. oryzae race 6. This gene was cloned using genetic means comparing Xanthomonas-sensitive and resistant strains of rice (Song et al. Science 270:1804-1806, 1995), and has been subsequently shown to confer resistance to Xanthomonas in Arabidopsis. The 1025 amino acid protein shows a number of features with similarity to known protein domains including a NH 2 -terminal 23 amino acid residue signal peptide, indicating that the protein is directed to the plasma membrane. Amino acids 81 to 634 contain 23 imperfect copies of a 24-amino acid LRR. Amino acids 651 to 676 encode a 26-amino acid hydrophobic segment that is likely to form a membrane-spanning domain. The C-terminal amino acids contain a putative intracellular serine threonine kinase domain carrying 11 subdomains with all 15 invariant amino acids that are typical of protein kinases. Subdomains VI and VIII are indicative of serine-threonine phosphorylation specificity. Xa21 has strong similarities to other RLKs, such as the Arabidopsis receptor-like kinase proteins RLK5 and TMK1, showing conservation of both the LRR and protein kinase domains. It is not yet known to what protein Xa21 transduces its pathogen recognition signal.

Another kind of membrane receptor molecules expressed by plant cells is histidine kinases (HKs). HKs have been known for some time in bacterial signal transduction systems, where they form one half of a two-component signaling system. The bacterial HK serves as a sensor molecule for extracellular signals, such as changes in osmoticum, nutrients and toxins. The HK autophosphorylates on a histidine residue in response to ligand binding. This phosphohistidine donates its phosphate group to an aspartate residue of the second member of the two component system, known as the response regulator (RR). The phosphorylated RR then binds DNA in a sequence-specific manner, serving to directly activate specific genes which code for proteins that mediate the response to the extracellular stimulus.

Like bacteria, plant cells have a two-component signaling system which consists of a sensor element HK and a RR. The two components may be separate molecules or may exist as a hybrid molecule (hereinafter referred to as hybrid HK/RR proteins). The HK proteins are distinguished by well-conserved amino acid motifs that occur in a specific order. From the amino terminus, the conserved regions are identified as the H, N, G1, F and G2 boxes. These motifs are usually found within a 200-250 amino acid span of the protein. The G1, F and G2 boxes are thought to be involved in nucleotide binding. As in bacteria, upon receiving the extracellular signal, the HK is autophosphorylated on the histidine residue contained in the H box. The phosphate group is subsequently transferred to the RR. All HKs are believed to phosphorylate a RR, as an obligate part of signal transduction. RRs are characterized by the absolute conservation of an aspartate which is phosphorylated by the phosphohistidine of the HK, and a conserved lysine residue. Unlike bacteria, RRs in plants have not been shown to bind DNA directly. Rather, all the plant RR's characterized to date appear to transduce the signal into protein kinase cascades, which eventually phosphorylate and either activate or inactivate transcription factors, and thereby gene expression.

The ethylene receptor (ETR1; Chang et al. Science 262:539-544) is the best known two-component signaling system in plants. Ethylene is a well known signal molecule that is involved in the regulation of plant development as well as the coordination of fertilization, senescence, skoto/photomorphogenesis and responses to pathogens and mechanical injury. The ethylene receptor is a hybrid HK/RR protein. The signal is transduced through the protein CTR1, which is a Raf-like protein kinase. CTR1 is a negative regulator of downstream steps in the signaling pathway. While the details of this pathway remain unclear, it appears that the HK is constitutively active in the absence of ethylene, thereby constantly phosphorylating CTR1, which in turn represses other genes in the ethylene response pathway. Binding of ethylene to ETR1 inhibits the HK function of the receptor, resulting in the inhibition of the negative regulator CTR1, thereby allowing the activation of downstream proteins in the ethylene signal transduction cascade. This culminates in activation of ethylene response genes.

Recently, two RR genes, IBC6 and IBC7, which are induced in response to the plant growth regulator cytokinin, have been cloned from Arabidopsis thaliana and characterized (Brandstatter and Kieber, The Plant Cell 10:1009-1019, 1998). It is likely that IBC6 and IBC7 are involved in the transduction of the cytokinin signal in plants. This is particularly interesting in light of the fact that a gene encoding the hybrid HK/RR protein CKI1 (Kakimoto, Science 274:982-985, 1996) causes cytokinin-like effects when it is ectopically expressed in transgenic plants. Thus it appears likely that a two-component HK/RR system is involved in cytokinin signal transduction. Cytokinin is known to regulate plant growth and development, including such physiological events as nutrient metabolism, expansion and senescence of leaves, and lateral branching.

While polynucleotides encoding proteins involved in plant cell signaling have been isolated for certain species of plants, genes encoding many such proteins have not yet been identified in a wide range of plant species. Thus, there remains a need in the art for materials which may be usefully employed in the modification of cell signaling in plants.

SUMMARY OF THE INVENTION

Briefly, the present invention provides polynucleotides isolated from eucalyptus and pine which encode polypeptides involved in cell signaling, together with methods for the use of such polynucleotides and polypeptides. Such polypeptides function as sensor-regulators or receptor kinases. The isolated polynucleotides and polypeptides may be usefully employed in the modification of plant cell responses either during the growth and development of a plant, or under conditions of stress resulting from pathogens or environmental factors.

In a first aspect, the present invention provides isolated and purified polynucleotides obtainable from eucalyptus and pine which encode RLKs, HKs, RRs or hybrid HK/RR proteins. In one embodiment, the isolated polynucleotides comprise a DNA sequence selected from the group consisting of: (a) sequences recited in SEQ ID NO: 1-67; (b) complements of the sequences recited in SEQ ID NO: 1-67; (c) reverse complements of the sequences recited in SEQ ID NO: 1-67; (d) reverse sequences of the sequences recited in SEQ ID NO: 1-67; and (e) sequences having either 40%, 60%, 75% or 90% identical nucleotides, as defined herein, to a sequence of (a)-(d).

In a further aspect, isolated polypeptides encoded by an inventive DNA sequence are provided. In one embodiment, such polypeptides comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 68-130.

In another aspect, the invention provides DNA constructs comprising a polynucleotide of the present invention, either alone, in combination with one or more other polynucleotides disclosed herein, or in combination with one or more known DNA sequences, together with transgenic cells comprising such constructs.

In a related aspect, the present invention provides DNA constructs comprising, in the 5′-3′ direction, a gene promoter sequence; an open reading frame coding for at least a functional portion of a polypeptide encoded by an inventive polynucleotide or a variant thereof; and a gene termination sequence. The open reading frame may be orientated in either a sense or antisense direction. DNA constructs comprising an untranslated, or non-coding, region of a gene coding for a cell membrane polypeptide encoded by the above polynucleotides or a nucleotide sequence complementary to a non-coding region, together with a gene promoter sequence and a gene termination sequence, are also provided. Preferably, the gene promoter and termination sequences are functional in a host plant. Most preferably, the gene promoter and termination sequences are those of the original genes but others generally used in the art, such as the Cauliflower Mosaic Virus (CaMV) promoter, with or without enhancers such as the Kozak sequence or Omega enhancer, and Agrobacterium tumefaciens nopaline synthase terminator may be usefully employed in the present invention. Tissue-specific promoters may be employed in order to target expression to one or more desired tissues. The DNA construct may further include a marker for the identification of transformed cells.

In a further aspect, transgenic cells, preferably plant cells, comprising the DNA constructs of the present invention are provided, together with organisms, preferably plants, comprising such transgenic cells, and fruit and seeds of such plants.

In yet another aspect, methods for modifying cell signaling in a target organism, such as a plant, are provided, such methods including stably incorporating into the genome of the plant a DNA construct of the present invention. In a preferred embodiment, the target plant is a woody plant, preferably selected from the group consisting of eucalyptus and pine species, most preferably from the group consisting of Eucalyptus grandis and Pinus radiata. In a related aspect, a method for producing a target organism, such as a plant, having modified cell signaling is provided, the method comprising transforming a plant cell with a DNA construct of the present invention to provide a transgenic cell and cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.

In yet a further aspect, the present invention provides methods for modifying the activity of a polypeptide in a target organism, such as a plant, comprising stably incorporating into the genome of the plant a DNA construct of the present invention. In a preferred embodiment, the target plant is a woody plant, preferably selected from the group consisting of eucalyptus and pine species, most preferably from the group consisting of Eucalyptus grandis and Pinus radiata.

The above-mentioned and additional features of the present invention and the manner of obtaining them will become apparent, and the invention will be best understood by reference to the following more detailed description. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually.

DETAILED DESCRIPTION

The present invention provides isolated and purified polynucleotides that encode proteins involved in plant cell signaling. As discussed above, cell signaling is known to play a critical role in the growth and development of plants, and in cellular responses to external stimuli, such as environmental factors and disease pathogens. Transformation of plants with polynucleotides that encode proteins involved in cell signaling may thus be employed to modify properties such as cell proliferation, differentiation, elongation and survival; resistance to disease; and nutrient metabolism.

For example, the HK ETR1 is known to be involved in ethylene signal transduction. Modulation of ETR1 expression will thus lead to a modification of physiological properties regulated by ethylene, such as fruit ripening, and senescence of leaves and flowers. Modulation of the expression of this protein in transgenic plants may therefore be employed to prolong the useful life of cut flowers by delaying senescence. Additionally, modulation of the expression of ETR1 could be used to selectively enhance the senescence of reproductive organs, resulting in engineered sterile plants.

The HK protein CKI1 has been implicated in cytokinin signal transduction. Its over expression is known to result in cytokinin-like effects in mutant plants. Cytokinin has been shown to play critical roles in lateral branching, leaf expansion, cell division, nutrient distribution, and delaying senescence, among other physiological phenomena. Therefore, modulation of the expression of CKI1 may result in, for example, the delay of senescence in selected cell types or organs. This would result in prolonged shelf life for fruits and vegetables between harvest and consumption. Alternatively, modulation of CKI1 expression may be used to decrease branching frequency in forest tree species, resulting in long stretches of valuable knot-free clear wood for use in solid timber furniture and veneers.

Using the methods and materials of the present invention, the amount of a specific plant cell polypeptide may be increased or reduced by incorporating additional copies of genes encoding the polypeptide into the genome of a target organism, such as a plant. Similarly, an increase or decrease in the amount of the polypeptide may be obtained by transforming the target organism with antisense copies of such genes.

In one embodiment, the present invention provides isolated polynucleotides encoding, or partially encoding, plant polypeptides that are involved in cell signaling, the polynucleotides being derived from eucalyptus and pine. Specifically, the present invention provides isolated polynucleotides encoding RLKs from Eucalyptus grandis (SEQ ID NO: 2, 8, 9, 11, 15, 18, 19, 21-25, 33, 34 and 38) and Pinus radiata (SEQ ID NO: 1, 3-7, 10, 12-14, 16, 17, 20, 26-32, 35-37, 39-41), and isolated polynucleotides encoding at least one member of a two-component signaling system (HKs, RRs or hybrid HK/RR proteins) from Eucalyptus grandis (SEQ ID NO: 42, 48-52, 55-58 and 67) and Pinus radiata (SEQ ID NO: 43-47, 53, 54 and 59-66). Complements of such isolated polynucleotides, reverse complements of such isolated polynucleotides and reverse sequences of such isolated polynucleotides are also provided, together with variants of such sequences, as defined below.

In another embodiment, the present invention provides isolated polypeptides encoded by the DNA sequences of SEQ ID NO: 1-67. In specific embodiments, such isolated polypeptides include an amino acid sequence of SEQ ID NO: 68-130. The present invention also encompasses DNA sequences that differ from the disclosed sequences but which, due to the degeneracy of the genetic code, encode a polypeptide which is the same as that encoded by a DNA sequence disclosed herein.

The term “polynucleotide(s),” as used herein, means a single or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases and includes DNA and corresponding RNA molecules, including HnRNA and mRNA molecules, both sense and anti-sense strands, and comprehends cDNA, genomic DNA and recombinant DNA, as well as wholly or partially synthesized polynucleotides. An HnRNA molecule contains introns and corresponds to a DNA molecule in a generally one-to-one manner. An mRNA molecule corresponds to an HnRNA and DNA molecule from which the introns have been excised. A polynucleotide may consist of an entire gene, or any portion thereof. Operable anti-sense polynucleotides may comprise a fragment of the corresponding polynucleotide, and the definition of “polynucleotide” therefore includes all such operable anti-sense fragments. Anti-sense polynucleotides and techniques involving anti-sense polynucleotides are well known in the art and are described, for example, in Robinson-Benion et al. (1995), Antisense techniques, Methods in Enzymol. 254(23): 363-375; and Kawasaki et al. (1996), Artific. Organs 20 (8): 836-848.

The term “polypeptide”, as used herein, encompasses amino acid chains of any length including full length proteins, wherein amino acid residues are linked by covalent peptide bonds. Polypeptides of the present invention may be purified natural products, or may be produced partially or wholly using recombinant or synthetic techniques.

All of the polynucleotides and polypeptides described herein are isolated and purified, as those terms are commonly used in the art.

The definition of the terms “complement”, “reverse complement” and “reverse sequence”, as used herein, is best illustrated by the following example. For the sequence 5′ AGGACC 3′, the complement, reverse complement and reverse sequence are as follows:

complement         3′ TCCTGG 5′
reverse complement 3′ GGTCCT 5′
reverse sequence   5′ CCAGGA 3′.

As used herein, the term “variant” covers any sequence which has at least about 40%, more preferably at least about 60%, more preferably yet at least about 75% and most preferably at least about 90% identical residues (either nucleotides or amino acids) to a sequence of the present invention. The percentage of identical residues is determined by aligning the two sequences to be compared, determining the number of identical residues in the aligned portion, dividing that number by the total length of the inventive, or queried, sequence and multiplying the result by 100.

Polynucleotide or polypeptide sequences may be aligned, and percentage of identical nucleotides in a specified region may be determined against another sequence, using computer algorithms that are publicly available. Two exemplary algorithms for aligning and identifying the similarity of polynucleotide sequences are the BLASTN and FASTA algorithms. The similarity of polypeptide sequences may be examined using the BLASTP algorithm. Both the BLASTN and BLASTP software are available on the NCBI anonymous FTP server (ftp://ncbi.nlm.nih.gov) under/blast/executables/. The BLASTN algorithm version 2.0.4 [Feb. 24, 1998], set to the default parameters described in the documentation and distributed with the algorithm, is preferred for use in the determination of variants according to the present invention. The use of the BLAST family of algorithms, including BLASTN and BLASTP, is described at NCBI's website at URL http://www.ncbi.nlm.nih.gov/BLAST/newblast.html and in the publication of Altschul, Stephen F., et al. (1997), “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs”, Nucleic Acids Res. 25:3389-3402. The computer algorithm FASTA is available on the Internet at the ftp site ftp://ftp.virginia.edu/pub/fasta/. Version 2.0u4, February 1996, set to the default parameters described in the documentation and distributed with the algorithm, is preferred for use in the determination of variants according to the present invention. The use of the FASTA algorithm is described in W. R. Pearson and D. J. Lipman, “Improved Tools for Biological Sequence Analysis,” Proc. Natl. Acad. Sci. USA 85:2444-2448 (1988) and W. R. Pearson, “Rapid and Sensitive Sequence Comparison with FASTP and FASTA,” Methods in Enzymology 183:63-98 (1990).

The following running parameters are preferred for determination of alignments and similarities using BLASTN that contribute to E values (as discussed below) and percentage identity: Unix running command: blastall -p blastn -d embldb -e 10 -G 1 -E 1 -r 2 -v 50 -b 50 -i queryseq -o results; and parameter default values:

-p Program Name [String]

-d Database [String]

-e Expectation value (E) [Real]

-G Cost to open a gap (zero invokes default behavior) [Integer]

-E Cost to extend a gap (zero invokes default behavior) [Integer]

-r Reward for a nucleotide match (blastn only) [Integer]

-v Number of one-line descriptions (V) [Integer]

-b Number of alignments to show (B) [Integer]

-i Query File [File In]

-o BLAST report Output File [File Out] Optional

For BLASTP the following running parameters are preferred: blastall -p blastp -d swissprotdb -e 10 -G 1 -E 1 -v 50 -b 50 -i queryseq -o results

-p Program Name [String]

-d Database [String]

-e Expectation value (E) [Real]

-G Cost to open a gap (zero invokes default behavior) [Integer]

-E Cost to extend a gap (zero invokes default behavior) [Integer]

-v Number of one-line descriptions (v) [Integer]

-b Number of alignments to show (b) [Integer]

-I Query File [File In]

-o BLAST report Output File [File Out] Optional

The “hits” to one or more database sequences by a queried sequence produced by BLASTN, BLASTP, FASTA, or a similar algorithm, align and identify similar portions of sequences. The hits are arranged in order of the degree of similarity and the length of sequence overlap. Hits to a database sequence generally represent an overlap over only a fraction of the sequence length of the queried sequence.

The BLASTN and FASTA algorithms also produce “Expect” or E values for alignments. The E value indicates the number of hits one can “expect” to see over a certain number of contiguous sequences by chance when searching a database of a certain size. The Expect value is used as a significance threshold for determining whether the hit to a database, such as the preferred EMBL database, indicates true similarity. For example, an E value of 0.1 assigned to a hit is interpreted as meaning that in a database of the size of the EMBL database, one might expect to see 0.1 matches over the aligned portion of the sequence with a similar score simply by chance. By this criterion, the aligned and matched portions of the sequences then have a 90% probability of being the same. For sequences having an E value of 0.01 or less over aligned and matched portions, the probability of finding a match by chance in the EMBL database is 1% or less using the BLASTN or FASTA algorithm.

According to one embodiment, “variant” polynucleotides, with reference to each of the polynucleotides of the present invention, preferably comprise sequences having the same number or fewer nucleic acids than each of the polynucleotides of the present invention and producing an E value of 0.01 or less when compared to the polynucleotide of the present invention. That is, a variant polynucleotide is any sequence that has at least a 99% probability of being the same as the polynucleotide of the present invention, measured as having an E value of 0.01 or less using the BLASTN or FASTA algorithms set at the parameters discussed above.

Variant polynucleotide sequences will generally hybridize to the recited polynucleotide sequence under stringent conditions. As used herein, “stringent conditions” refers to prewashing in a solution of 6×SSC, 0.2% SDS; hybridizing at 65° C., 6×SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in 1×SSC, 0.1% SDS at 65° C. and two washes of 30 minutes each in 0.2×SSC, 0.1% SDS at 65° C.

As used herein, the term “x-mer,” with reference to a specific value of “x,” refers to a polynucleotide comprising at least a specified number (“x”) of contiguous residues of any of the polynucleotides identified as SEQ ID NO: 1-67. The value of x may be from about 20 to about 600, depending upon the specific sequence.

Polynucleotides of the present invention comprehend polynucleotides comprising at least a specified number of contiguous residues (x-mers) of any of the polynucleotides identified as SEQ ID NO: 1-67 or their variants. According to preferred embodiments, the value of x is preferably at least 20, more preferably at least 40, more preferably yet at least 60, and most preferably at least 80. Thus, polynucleotides of the present invention include polynucleotides comprising a 20-mer, a 40-mer, a 60-mer, an 80-mer, a 100-mer, a 120-mer, a 150-mer, a 180-mer, a 220-mer a 250-mer, or a 300-mer, 400-mer, 500-mer or 600-mer of a polynucleotide identified as SEQ ID NO: 1-67 or a variant of one of the polynucleotides identified as SEQ ID NO: 1-67.

The inventive polynucleotides may be isolated by high throughput sequencing of cDNA libraries prepared from Eucalyptus grandis and Pinus radiata as described below in Examples 1 and 2. Alternatively, oligonucleotide probes based on the sequences provided in SEQ ID NO: 1-67 can be synthesized and used to identify positive clones in either cDNA or genomic DNA libraries from Eucalyptus grandis and Pinus radiata by means of hybridization or PCR techniques. Probes can be shorter than the sequences provided herein but should be at least about 10, preferably at least about 15 and most preferably at least about 20 nucleotides in length. Hybridization and PCR techniques suitable for use with such oligonucleotide probes are well known in the art, and include those taught by Sambrook et al., Ibid. Positive clones may be analyzed by restriction enzyme digestion, DNA sequencing or the like.

In addition, the DNA sequences of the present invention may be generated by synthetic means using techniques well known in the art. Equipment for automated synthesis of oligonucleotides is commercially available from suppliers such as Perkin Elmer/Applied Biosystems Division (Foster City, Calif.) and may be operated according to the manufacturer's instructions.

In one embodiment, the DNA constructs of the present invention include an open reading frame coding for at least a functional portion of a polypeptide encoded by a polynucleotide of the present invention or a variant thereof. As used herein, the “functional portion” of a polypeptide is that portion which contains the binding site or the catalytic signal transduction site of the protein. The functional portion can be determined by targeted mutagenesis and screening of modified protein products with protocols well known in the art. Normally, the functional portion is 10-20 amino acids in length, but can be shorter or longer. The active site may be made up of separate portions present on one or more polypeptide chains and will generally exhibit high substrate specificity. The term “polypeptide encoded by a polynucleotide” as used herein, includes polypeptides encoded by a nucleotide sequence which includes the partial isolated DNA sequences of the present invention.

The open reading frame is inserted in the DNA construct in a sense or antisense orientation, such that transformation of a target plant with the DNA construct will lead to a change in the amount of polypeptide compared to the wild-type plant. Transformation with a DNA construct comprising an open reading frame in a sense orientation will generally result in over-expression of the selected gene, while transformation with a DNA construct comprising an open reading frame in an antisense orientation will generally result in reduced expression of the selected gene. A population of plants transformed with a DNA construct comprising an open reading frame of the present invention in either a sense or antisense orientation may be screened for increased or reduced expression of the gene in question using techniques well known to those of skill in the art, and plants having the desired phenotypes may thus be isolated.

Alternatively, expression of a gene involved in plant cell signaling may be inhibited by inserting a portion of an open reading frame of the present invention, in either sense or antisense orientation, in the DNA construct. Such portions need not be full-length but preferably comprise at least 25 and more preferably at least 50 residues of an inventive DNA sequence. A longer portion or even the full length DNA corresponding to the complete open reading frame may be employed. The portion of the open reading frame does not need to be precisely the same as the endogenous sequence, provided that there is sufficient sequence similarity to achieve inhibition of the target gene. Thus a sequence derived from one species may be used to inhibit expression of a gene in a different species.

In a second embodiment, the inventive DNA constructs comprise a DNA sequence including an untranslated region of a gene coding for a polypeptide encoded by a polynucleotide of the present invention, or a DNA sequence complementary to such untranslated region. Examples of untranslated regions which may be usefully employed in such constructs include introns and 5′-untranslated leader sequences. Transformation of a target plant with such a DNA construct may lead to a reduction in the amount of the polypeptide expressed in the plant by the process of cosuppression, in a manner similar to that discussed, for example, by Napoli et al. ( Plant Cell 2:279-290, 1990) and de Carvalho Niebel et al. ( Plant Cell 7:347-358, 1995).

Alternatively, regulation of polypeptide expression can be achieved by inserting appropriate sequences or subsequences (e.g. DNA or RNA) in ribozyme constructs (McIntyre C L, Manners J M, Transgenic Res., 5(4): 257-262, 1996). Ribozymes are synthetic RNA molecules that comprise a hybridizing region complementary to two regions, each of which comprises at least 5 contiguous nucleotides of a mRNA molecule encoded by one of the inventive polynucleotides. Ribozymes possess highly specific endonuclease activity, which autocatalytically cleaves the mRNA.

The DNA constructs of the present invention further comprise a gene promoter sequence and a gene termination sequence, operably linked to the DNA sequence to be transcribed, which control expression of the gene. The gene promoter sequence is generally positioned at the 5′ end of the DNA sequence to be transcribed, and is employed to initiate transcription of the DNA sequence. Gene promoter sequences are generally found in the 5′ non-coding region of a gene but they may exist downstream of the open reading frame, in introns (Luehrsen, K. R., Mol. Gen. Genet. 225:81-93, 1991) or in the coding region, as for example in a plant defense gene (Douglas et al. EMBO J. 10:1767-1775, 1991).

A variety of gene promoter sequences which may be usefully employed in the DNA constructs of the present invention are well known in the art. The gene promoter sequence, and also the gene termination sequence, may be endogenous to the target plant host or may be exogenous, provided the promoter is functional in the target host. For example, the promoter and termination sequences may be from other plant species, plant viruses, bacterial plasmids and the like. Preferably, gene promoter and termination sequences are from the inventive sequences themselves.

Factors influencing the choice of promoter include the desired tissue specificity of the construct, and the timing of transcription and translation. For example, constitutive promoters, such as the Cauliflower Mosaic Virus 35S (CaMV 35S) promoter, will affect the activity of the enzyme in all parts of the plant. Use of a tissue specific promoter will result in production of the desired sense or antisense RNA only in the tissue of interest. With DNA constructs employing inducible gene promoter sequences, the rate of RNA polymerase binding and initiation can be modulated by external stimuli, such as light, heat, anaerobic stress, alteration in nutrient conditions and the like. Temporally regulated promoters can be employed to effect modulation of the rate of RNA polymerase binding and initiation at a specific time during development of a transformed cell. Preferably, the original promoters from the gene in question, or promoters from a specific tissue-targeted gene in the organism to be transformed, such as eucalyptus or pine, are used. Other examples of gene promoters which may be usefully employed in the present invention include mannopine synthase (mas), octopine synthase (ocs) and those reviewed by Chua et al. ( Science, 244:174-181, 1989).

The gene termination sequence, which is located 3′ to the DNA sequence to be transcribed, may come from the same gene as the gene promoter sequence or may be from a different gene. Many gene termination sequences known in the art may be usefully employed in the present invention, such as the 3′ end of the Agrobacterium tumefaciens nopaline synthase gene. In one embodiment, terminator sequences are those from the original enzyme gene or from the target species to be transformed.

The DNA constructs of the present invention may also contain a selection marker that is effective in plant cells, to allow for the detection of transformed cells containing the inventive construct. Such markers, which are well known in the art, typically confer resistance to one or more toxins. One example of such a marker is the NPTII gene whose expression results in resistance to kanamycin or hygromycin, antibiotics which are usually toxic to plant cells at a moderate concentration (Rogers et al. in Methods for Plant Molecular Biology, A. Weissbach and H. Weissbach, eds., Academic Press Inc., San Diego, Calif. (1988)). Transformed cells can thus be identified by their ability to grow in media containing the antibiotic in question. Alternatively, the presence of the desired construct in transformed cells can be determined by means of other techniques well known in the art, such as Southern and Western blots.

Techniques for operatively linking the components of the inventive DNA constructs are well known in the art and include the use of synthetic linkers containing one or more restriction endonuclease sites as described, for example, by Sambrook et al., ( Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y., 1989). The DNA construct of the present invention may be linked to a vector having at least one replication system, for example E. coli, whereby after each manipulation, the resulting construct can be cloned and sequenced and the correctness of the manipulation determined.

The DNA constructs of the present invention may be used to transform a variety of target organisms, including plants, both monocotyledonous angiosperms(e.g. grasses, corn, grains, oat, wheat and barley), dicotyledonous angiosperms (e.g. Arabidopsis, tobacco, legumes, alfalfa, oaks, eucalyptus, maple), and Gymnosperms (e.g. Scots pine (Aronen, Finnish Forest Res. Papers, vol. 595, 1996), white spruce (Ellis et al., Biotechnology 11:94-92, 1993), and larch (Huang et al., In Vitro Cell 27:201-207, 1991)). In a preferred embodiment, the inventive DNA constructs are employed to transform woody plants, herein defined as a perennial tree or shrub whose stem increases in diameter each year by the addition of woody tissue. Preferably the target plant is selected from the group consisting of eucalyptus and pine species, most preferably from the group consisting of Eucalyptus grandis and Pinus radiata. Other species which may be usefully transformed with the DNA constructs of the present invention include, but are not limited to: pines such as Pinus banksiana, Pinus brutia, Pinus caribaea, Pinus clausa, Pinus contorta, Pinus coulteri, Pinus echinata, Pinus eldarica, Pinus ellioti, Pinus jeffreyi, Pinus lambertiana, Pinus monticola, Pinus nigra, Pinus palustrus, Pinus pinaster, Pinus ponderosa, Pinus resinosa, Pinus rigida, Pinus serotina, Pinus strobus, Pinus sylvestris, Pinus taeda, Pinus virginiana; other gymnosperms, such as Abies amabilis, Abies balsamea, Abies concolor, Abies grandis, Abies lasiocarpa, Abies magnifica, Abies procera, Chamaecyparis lawsoniona, Chamaecyparis nootkatensis, Chamaecyparis thyoides, Huniperus virginiana, Larix decidua, Larix laricina, Larix leptolepis, Larix occidentalis, Larix siberica, Libocedrus decurrens, Picea abies, Picea engelmanni, Picea glauca, Picea mariana, Picea pungens, Picea rubens, Picea sitchensis, Pseudotsuga menziesii, Sequoia gigantea, Sequoia sempervirens, Taxodium distichum, Tsuga canadensis, Tsuga heterophylla, Tsuga mertensiana, Thuja occidentalis, Thuja plicata; Eucalypts, such as Eucalyptus alba, Eucalyptus bancroftii, Eucalyptus botyroides, Eucalyptus bridgesiana, Eucalyptus calophylla, Eucalyptus camaldulensis, Eucalyptus citriodora, Eucalyptus cladocalyx, Eucalyptus coccifera, Eucalyptus curtisii, Eucalyptus dalrympleana, Eucalyptus deglupta, Eucalyptus delagatensis, Eucalyptus diversicolor, Eucalyptus dunnii, Eucalyptus ficifolia, Eucalyptus globulus, Eucalyptus gomphocephala, Eucalyptus gunnii, Eucalyptus henryi, Eucalyptus laevopinea, Eucalyptus macarthurii, Eucalyptus macrorhyncha, Eucalyptus maculata, Eucalyptus marginata, Eucalyptus megacarpa, Eucalyptus melliodora, Eucalyptus nicholii, Eucalyptus nitens, Eucalyptus nova-anglica, Eucalyptus obliqua, Eucalyptus obtusiflora, Eucalyptus oreades, Eucalyptus pauciflora, Eucalyptus polybractea, Eucalyptus regnans, Eucalyptus resinifera, Eucalyptus robusta, Eucalyptus rudis, Eucalyptus saligna, Eucalyptus sideroxylon, Eucalyptus stuartiana, Eucalyptus tereticornis, Eucalyptus torelliana, Eucalyptus urnigera, Eucalyptus urophylla, Eucalyptus viminalis, Eucalyptus viridis, Eucalyptus wandoo and Eucalyptus youmanni; and hybrids between any of the above species.

Techniques for stably incorporating DNA constructs into the genome of target plants are well known in the art and include Agrobacterium tumefaciens mediated introduction, electroporation, protoplast fusion, injection into reproductive organs, injection into immature embryos, high velocity projectile introduction and the like. The choice of technique will depend upon the target plant to be transformed. For example, dicotyledonous plants and certain monocots and gymnosperms may be transformed by Agrobacterium Ti plasmid technology, as described, for example by Bevan ( Nucl. Acid Res. 12:8711-8721, 1984). Targets for the introduction of the DNA constructs of the present invention include tissues, such as leaf tissue, dissociated cells, protoplasts, seeds, embryos, meristematic regions; cotyledons, hypocotyls, and the like. One method for transforming eucalyptus and pine is a biolistic method using pollen (see, for example, Aronen 1996, Finish Forest Res. Papers vol. 595, 53pp) or easily regenerable embryonic tissues.

Once the cells are transformed, cells having the inventive DNA construct incorporated in their genome may be selected by means of a marker, such as the kanamycin resistance marker discussed above. Transgenic cells may then be cultured in an appropriate medium to regenerate whole plants, using techniques well known in the art. In the case of protoplasts, the cell wall is allowed to reform under appropriate osmotic conditions. In the case of seeds or embryos, an appropriate germination or callus initiation medium is employed. For explants, an appropriate regeneration medium is used. Regeneration of plants is well established for many species. For a review of regeneration of forest trees see Dunstan et al., Somatic embryogenesis in woody plants. In: Thorpe, T. A. ed., 1995: In Vitro Embryogenesis of Plants. Vol. 20 in Current Plant Science and Biotechnology in Agriculture, Chapter 12, pp. 471-540. Specific protocols for the regeneration of spruce are discussed by Roberts et al., (Somatic Embryogenesis of Spruce. In: Synseed. Applications of synthetic seed to crop improvement. Redenbaugh, K., ed. CRC Press, Chapter 23, pp. 427-449, 1993). The resulting transformed plants may be reproduced sexually or asexually, using methods well known in the art, to give successive generations of transgenic plants.

As discussed above, the production of RNA in target plant cells can be controlled by choice of the promoter sequence. A target plant may be transformed with more than one DNA construct of the present invention, thereby modulating the activity of more than one polypeptide, affecting polypeptide activity in more than one tissue, or affecting polypeptide activity at more than one expression time. Similarly, a DNA construct may be assembled containing more than one open reading frame coding for a polypeptide encoded by a polynucleotide of the present invention or more than one untranslated region of a gene coding for such a polypeptide. The polynucleotides of the present invention may also be employed in combination with other known sequences encoding polypeptides involved in plant cell signaling.

The isolated polynucleotides of the present invention may also be employed as probes to isolate DNA sequences encoding polypeptides involved in cell signaling from other plant species, using techniques well known to those of skill in the art, such as routinely used DNA hybridization and PCR techniques.

The inventive polynucleotides, polypeptides expressed by such polynucleotides and antibodies to such polypeptides may be used to screen for molecules that interact with such polynucleotides and/or polypeptides and that thereby modulate cell signaling. Techniques for performing such assays are well known in the art. Similarly, the polynucleotides and corresponding expressed polypeptides of the present invention may be employed in studies designed to elucidate the mechanism of cell signaling pathways.

The following examples are offered by way of illustration and not by way of limitation.

EXAMPLE 1

Isolation and Characterization of cDNA Clones from Eucalyptus grandis

Eucalyptus grandis cDNA expression libraries were constructed and screened as follows.

mRNA was extracted from specific plant tissues, such as trunk xylem, using the protocol of Chang et al. ( Plant Molecular Biology Reporter 11:113-116 (1993)) with minor modifications. Specifically, samples were dissolved in CPC-RNAXB (100 mM Tris-Cl, pH 8,0; 25 mM EDTA; 2.0 M NaCl; 2% CTAB; 2% PVP and 0.05% Spermidine*3 HCl) and extracted with chloroform:isoamyl alcohol, 24:1. mRNA was precipitated with ethanol and the total RNA preparate was purified using a Poly(A) Quik mRNA Isolation Kit (Stratagene, La Jolla, Calif.). A CDNA expression library was constructed from the purified mRNA by reverse transcriptase synthesis followed by insertion of the resulting cDNA clones in Lambda ZAP using a ZAP Express cDNA Synthesis Kit (Stratagene), according to the manufacturer's protocol. The resulting cDNAs were packaged using a Gigapack II Packaging Extract (Stratagene) employing 1 μl of sample DNA from the 5 μl ligation mix. Mass excision of the library was done using XL1-Blue MRF′ cells and XLOLR cells (Stratagene) with ExAssist helper phage (Stratagene). The excised phagemids were diluted with NZY broth (Gibco BRL, Gaithersburg, Md.) and plated out onto LB-kanamycin agar plates containing X-gal and isopropylthio-beta-galactoside (IPTG).

Of the colonies plated and picked for DNA miniprep, 99% contained an insert suitable for sequencing. Positive colonies were cultured in NZY broth with kanamycin and cDNA was purified by means of alkaline lysis and polyethylene glycol (PEG) precipitation. Agarose gel at 1% was used to screen sequencing templates for chromosomal contamination. Dye primer sequences were prepared using a Turbo Catalyst 800 machine (Perkin Elmer/Applied Biosystems Division, Foster City, Calif.) according to the manufacturer's protocol.

DNA sequence for positive clones was obtained using a Perkin Elmer/Applied Biosystems Division Prism 377 sequencer. cDNA clones were sequenced first from the 5′ end and, in some cases, also from the 3′ end. For some clones, internal sequence was obtained by designing primers to hybridise to the ends of known sequences, and using these as sequencing primers extending the amount of sequence information. This procedure was repeated iteratively until the complete sequence was obtained. Alternatively, internal sequence was obtained by generating “nested” deletion clones of the gene of interest using published methods (Henikoff, (1984) Gene 28 351-359).

The determined cDNA sequence was compared to known sequences in the EMBL database (up to end of August 1998) using the computer algorithms FASTA and/or BLASTN. Multiple alignments of redundant sequences were used to build up reliable consensus sequences. Based on similarity to known sequences from other plant species, the isolated DNA sequences were identified as encoding RLKs (SEQ ID NO: 2, 8, 9, 11, 15, 18, 19, 21-25, 33, 34 and 38) or at least one member of a two-component signaling system (HKs, RRs or hybrid HK/RR proteins; SEQ ID NO: 42, 48-52, 55-58 and 67). The sequences of SEQ ID NO: 2, 8, 9, 11, 15, 18, 19, 21-25, 33, 34 and 38 were found to have less than 10% identical residues (determined as described above) to known sequences.

EXAMPLE 2

Isolation and Characterization of cDNA Clones from Pinus radiata

A Pinus radiata cDNA expression library was constructed from xylem and screened as described above in Example 1. DNA sequence for positive clones was obtained using forward and reverse primers on a Perkin Elmer/Applied Biosystems Division Prism 377 sequencer and the determined sequences were compared to known sequences in the database as described above.

Based on similarity to known sequences from other plant species, the isolated DNA sequences were identified as encoding RLKs (SEQ ID NO: 1, 3-7, 10, 12-14, 16, 17, 20, 26-32, 35-37 and 39-41) or at least one member of a two-component signaling system (HKs, RRs or hybrid HK/RR proteins; SEQ ID NO: 43-47, 53, 54 and 59-66). The sequences of SEQ ID NO: 3-7, 10, 12-14, 16, 17, 20, 26, 28-32, 35-37 and 39-41 were found to have less than 10% identical residues (determined as described above) to known sequences.

EXAMPLE 3

Use of an Ethylene Receptor Gene to Modify Plant Growth

Transformation of tobacco plants with a Pinus radiata ethylene receptor gene homolog is performed as follows. DNA constructs comprising sense and anti-sense constructs containing a DNA sequence including the coding region of an ethylene receptor homolog (SEQ ID NO: 43) from Pinus radiata are constructed and inserted into Agrobacterium tumefaciens by direct transformation using published methods (see, An G, Ebert P R, Mitra A, Ha S B: Binary Vectors. In: Gelvin S B, Schilperoort RA (eds) Plant Molecular Biology Manual, Kluwer Academic Publishers, Dordrecht (1988)). The constructs of sense DNAs are made by direct cloning from PBK-CMV plasmid by cloning cDNA insert into pART7 plasmid, which is then cut by NotI enzyme and 35S-Insert-OCS 3′UTR put into pART27 plant expression vector (See Gleave, A. Plant Molecular Biology 20:1203-1207, 1992). The presence and integrity of the transgenic constructs are verified by restriction digestion and DNA sequencing.

Tobacco ( Nicotiana tabacum cv. Samsun) leaf sections are transformed with the sense and anti-sense ethylene receptor constructs using a method based on that of Horsch et al. ( Science, 227:1229-1231, 1985). Transformed plants containing the appropriate construct are verified using Southern blot experiments. Expression of the Pinus ethylene receptor homolog in transformed plants is confirmed by isolating total RNA from each independent transformed plant line created with the sense and anti-sense constructs. The RNA samples are analysed in Northern blot experiments to determine the level of expression of the transgene in each transformed line. The expression level of the ethylene receptor polypeptide, encoded by the Pinus ethylene receptor gene and by the endogenous tobacco ethylene receptor gene, for each transformed plant line created with the sense and anti-sense constructs is compared to that of wild-type control plants.

Although the present invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, changes and modifications can be carried out without departing from the scope of the invention which is intended to be limited only by the scope of the claims.

130 1 2389 DNA Pinus radiata 1atctactggt agtgctaatc ccaatctcat tccagtgggt cattaacatg ggctgagagg 60tttcaaaatc tttatatggt tgttgaagta tccacttcct gtgccaatct tattctcatt 120ctctgccgtt acaatggaga ggctggagat ggcgatgttt ttaatgttac tgattatctt 180tttctttaga gattgtgaag cccagggcaa atcggattat catgcactga tagctttcaa 240ggcctcctct gatatcaata acactctggc gttctcatgg acacataaga atccatgcag 300acgaaagtgg tatggagttg ggtgtgagaa tggccgggta gtgaggcttg tgctccagga 360tctcgacctc gtgggtccga ttgatgcctt aacaggtctc catgagctca gaattctgag 420cttgaagaga aattcactca atggcaccat tcctgatttt ttgaactgga gaagtcttaa 480gttcctgttt ctctctgata actatttctc aggtcccttg ccttctacca ttgcttcttt 540agatcatctc ttgagattgg atgtatcgaa caatcatctt ggcggtcaaa taccattgag 600tattacttca atgacccatt tgcttactct gaggcttgag aacaatgagt tctctggcag 660catttcagac ttgatgttgc ccaatactgt ggaagaattc aatgtttcag acaatagact 720tagtggcatg atacctgcaa gtctgtctag gtttccaagc tctggatttg tgaataatga 780agaactctgt ggaagtcccc tgcagagttg caatgaaagt aacaatgcaa gctctgcttc 840taatccctac atggcttcca gcccctccat gatttcaggc tcactgccag tgcatagaaa 900taatagctct acgcaactca gcaaaggtga tattatagca atagttgtgg gagatgttgc 960agtgttatcg ctcataggtt gtgtaatctt ctgctactac tggaaaaaga agggtgtaaa 1020acagaagaag cctaagccta agcctgccca gagatgtcca acagacaggt tagctgtgca 1080ttcttcagat cagtgcccta ataatcagtc tattactgca ggaaaatgca agcttatctt 1140ctttgatgat ggaagacctt ttgagctaga gcatctcttg cgagcttcgg ctgaaatgct 1200aggaaagggt aactttggaa gcgcttataa ggctattatg gaagatggat ctgttgttgc 1260tgtcaagaga ttgaaagatt tgtatggtat tggaaggaag gagtttgagc aacatatgga 1320gctcatggga agtttgagac atcaaaatgt agttaattta agggcctatt attttgctag 1380agatgaaaag cttctggtct atgactatat gccaaatggg agtctgtatg cacttcttca 1440tggtagccga ggacctggta gaacacccct agactggaca actagaatga agatagcact 1500tggtgcagct aaaggcttgg catttattca cagccactgc aagtcaccca agattggcca 1560cggaaacatc aagtcttcca atattttgtt agacagaaat gggaatgctt gcatttcaga 1620ctttggcctt gcactgctgg tgagtccatc agttgctgct tcacgaatgg tgggctacac 1680tgctcctgag caagctgcaa caaagaagat atcccagaaa gcggacgtgt acagctttgg 1740agttttactg cttgaaatgc taactggcaa ggcccctgtt caagctcaca tgcaagagga 1800ttatcattca gctatagatc ttcccaggtg ggtgcagtcc attgtcccag aagaatggac 1860atcagaggta tttgatattg agttgatgag attcaaaaac attgaagagg aactagtaag 1920catgcttcag attgctttgc tctgtgcctc acaatctcct cagcaacgcc ctaaaatgag 1980ccatgtggtg agggtgattc aagacattag aggagatcac cactctccct ccatgcagaa 2040ttctctatct caatctcctt ccatgcaaga accaggtcat tctatatcag attctccttc 2100tgtatcagag gactcgggaa taagaggcct gtgagtttgt gtatttgttc tcattagaat 2160ccaatttgct tgaatttaat tgcaggtatt ctttagcagc atccttttgg atgggcattt 2220aactagctgt agcataagag tgtagcatat gcttaaatgg gcatttaagt atatgcagtg 2280tgtaggagga ggtatatttt agaagacttg gatttggttt tttcttgaag tatccatatc 2340catattaaaa gagttggtat tttggttaag atccaaaaaa aaaaaaaaa 2389 2 2568 DNA Eucalyptus grandis 2agagagatgg gtttcgcttc cttgcgctcg cagtgtctct tcttcttcct cctctgggtc 60ttcatcttct tcgccagccg aagcgacgtg cttgtctccg cagcaacgag cgcggagctg 120agagctctca tggacatgaa agccagccta gacccggaaa gcaggtatct ttcctcgtgg 180accgtcgatg gcgatccttg cgacgggtcc ttccaaggcg tctgctgcga cgacgagggc 240cgcgtcgcca acgtttcgct gcaggggaga cggctcaccg ggaggctctc tccggccatt 300gctgggctca cccacttgac tggcctttac cttcactaca actccctgtg cggagagatt 360ccgcgagagc tcggcaactt gagcgcgctc agcgacttgt atctgaacat gaacaatctc 420tccggccaga ttcctcccga gatggctgac gtcgcaagct tgcaagttat gcagctctct 480tacaaccagt tgactggaag tatacctaca aagctgggat ctctgaagaa gctcagtgtt 540cttgctctgc aatctaatca gctcactggc gcaataccgg caagtttagg tgacctgggg 600acgttgacga ggttatattt gagtttcaat cgcctctttg gctcgattcc catgaagata 660gccgatctgc ctcttctaga agtcttggat gtccaaaaca acactctctc tggcaatgta 720cctcctgcgc tgaagagact gaacgaagga tttctgtacg aaaacaactt cgacttgtgt 780gggactgggt tcctctcttt aaggacttgt aatgctttgg agggtcgaaa gcctagtcaa 840cctcaaccct atggggctgc gacaacagta ccctccacaa gtatacccga gacggccaat 900gtggtgttac catgtaacct gactgaatgt tctagtttgc caaaatcagc gcatccctct 960gcgcttattg gatcaatctt ggcaactgtt gcattgtcgg ccatagggtt ccttttgttc 1020acacactacc gtcggaggaa acagaaactt ggcttttcgg ctgaggtttg tgatggccat 1080ctgagcactg atcaacccaa gagtgcttat aagaaaaatg gctctccttt agctagcctg 1140gagtattcca acggatggga tccgcttgct gatgctcgga tctttaatga gttttctgaa 1200gaagctttcc agagcttcag attcaatctg gaagaggttg aatccgccac tcagtatttc 1260tcggagttga atgtgttggg taagagtaat ttctctacca catacagagg aatcctgaga 1320gacggttctg tagtctccat caaatgcatc aataagacta gctgcaaggc tgatgaatca 1380gagttcttga agggtttgaa tatgttgacc tcactgagac atgaaaatct ggtgaggctg 1440agaggattct gctgctcaac ggcccgtggt gagtgcttcc tgatatatga ttatgttccg 1500aatgggactc tgctaagctt tttggatttg gaggaggggg acagtggcac tcttgagtgg 1560tccactagag tttcgattgt gaagggaatc gctaaaggta ttgcctattt gcacgctcac 1620aaaccaaaca aggcccccct ccttcaccaa aacatctcag cagacaaggt gctcatagac 1680cagcgattca atccgttgct ctaccaatct ggtctccaca ggctcctgac aaatgatgtt 1740gtgttctcgt tgctcaaagc tagtgctgcc atgggctacc tagcgcctga gtacatgtcc 1800acgggccggt tcacagagaa gagtgatgtc tacgcgtttg gaatgatcgt gttccagatc 1860ttgtcgggga agcagaaagt agatcactcc atgcgtctgg ctgctgaatc gtgtagattc 1920caagaattca ttgatgcgaa tatccatgga aggttcttcg agtacgaggc agctaaactt 1980gcaaaaatcg cctcgctctg caccaacgag tctccttatg acaggccatc catggacgcc 2040gtgattcacg agctgagtaa ctgtagcagc tgtctctgat tgtgaggcct tattggagta 2100aatgttccag gagctgttgc aaaagtcaat ggtagaagtt ggtctgaaaa agctccctgc 2160gctgccacta atgttgtaac ctaccggctt tgacatcttt ttgatttggt gcctttgcct 2220tagggccgtg ctttcttgtg tatgttcaag cctggtaggg atgaatgact ttaattcacg 2280catgtatgat tgcttttcaa aatattttct ttagaagtag gttcatttcg gaattagatt 2340ttcgatattt ctcgttgcgt gccgcagttg tgtgggtgaa aatggccgtg gacttccaac 2400gccggtaact agccttgttg ccgtggtcgc attgcattag ccctgcctgg catctttaat 2460tgctggttaa ttgaggcgat ttgtttttcc attggtggat ctgctggcat ctttaattgc 2520tggttaatcg aggcgatttg tttttccgta aaaaaaaaaa aaaaaaaa 2568 3 2686 DNA Pinus radiata 3gacgaggatc cccaaggccc attgctttat tgcctggaaa gaattttaaa gcttatatat 60atattattgt tgctctataa tagtaatgat aaatttaggc ctaggcatag gtgtgtcatt 120gccattctta gaagcaatgc ctggcactgg tcactgccca cagtccagct ctcaaagcct 180gtcctgtagc tgttgtctca atgccttgtc ttactttgtg agctggtgtc tgtcatggag 240gtagaggaag aatcatttgg cttttaagtt ttaccccatt tttcttcagt aagaagaagt 300gcctccatgg tttgcagcag atcggagctt tgctgagacg cgattaggta acccgtctgc 360gacaattttt tctctgcaat gtgggtttgt tgggcatttt acagaagcat gactggcaga 420gaagaatagc agcaagtata tatctctgtt tttggcttgc tgtgatgtat tggtcgcaga 480tggggaatat acggcagctt tttaatggtt tcttcatgct ggtgctagta gtggccgtgg 540ttaaggggga gccaacgggc gataaacagg cccttctcga ttttctcagc aaggttcccc 600atgggcgaag gctaaactgg aatgccagca gctcagcttg tacttgggtt ggagtgacct 660gcaatagtaa caaggatcgc atctgggaag ttagattgcc gggcgtggga ctctttggac 720cgattccccc tggcactctg gggcgtctga cggagctcag agttctttcc ctgagatcaa 780atttgctcac cggttctctg ccttcagacc ttgcaaatgc caaggctctg agaagtatct 840atcttcagca taacttgttt tccggaccat tgcctccgtt tctgtctcaa tgggggcgct 900tgagtcgact ggatctttcc ttcaatcgtt tgaatgggtc tattcccttt tccttgaata 960acctcacgca tctcactggt ctcttgttgc agaacaattc gctgagtgga agcataccca 1020atctcaacat ccagaacttg actctgttga gtgtggccaa caatcagctc aacggttcga 1080ttcctaggtc gcttcagaag tttcccaaga cttctttcca aggcaatgct cagctctgcg 1140gggttccatt aaaactgtgc aaatcgtttt tcccgtcgcc ttctccctct cccaacggtt 1200ctgctgtgcc ccggaggtcc aagaaatcga agctgagcac cggcgtggtc gtggctatca 1260tcgtgggcgc cgttgctgtg ctcttcttgt tgctggcatg cctatttttg tgctgcgtta 1320gaaagcacag gggcgagagt gcgacggaga agcctcagaa agacgagcgg accacggtcg 1380agaagggcgg gccttctaag gaggaataca tggggactgc ccaggagaca gagcgcaata 1440agctcgtatt cttcgagggc agccagtata ctttcgacct ggaagatctt ctccgcgctt 1500ctgcagaggt tctggggaaa ggaagcgtgg ggacggccta caaggcagtg ctggaagatg 1560ggactactgt tgtggtcaaa cgcttgaagg acgtggctgt caacaggagg gattttgaac 1620agcagatgga gctggtggga aggatccgtc acaggaattt ggtgcccttg agagccttct 1680acttttccaa agatgagaaa ttactcgtct atgactatat gcccgctggc agcttatcag 1740cccttttgca tggtagcaga ggctctggcc gcacaccttt ggattgggaa accaggatgc 1800ggattgcttt gggtgctgca aggggcattt cccacatcca cgaagaaggc ggaggcaaat 1860tcacccatgg aaacataaag tcatccaatg ttctgttaac gtccgatttg gatggctgcg 1920tctcagactt cggcctcgtt ccattattta gcgctgctgc agcagcaaac cgcatcgctg 1980gttacagagc cccggaggtc atagagactc gcaaggtcac acagaaatcc gatgtctaca 2040gcttcggtgt gctactccta gaacttctca caggcaaggc tcccaaccag gcctctctca 2100acgacgaggg catcgatctg ccaagatggg tacaatccgt ggttcgcgag gagtggacag 2160cagaggtttt cgacgtggaa ttaatgaggt accaaaacat agaagaagag atggtccagt 2220tgctgcagat tgccatggcc tgcgttgcta cagtgcctga tcagaggccc agaatgcaag 2280acgtggtgaa gatgatcgag gacatgaggc agttcgaaac agacgaagga aatagacaat 2340cctctgatga taaatcgaaa gaatccaatg gacaaactcc tccccagcaa gccacaccag 2400aagcacgcac ccccacagca agaacaccgt gacagcaccc cctcagcaag cacactttga 2460cagtcgaaat tcttctgttt ctgtccatat tataacacag ttgccagtat catgggcttt 2520tcgtgattga tttcatttat atccagtatt tcagtaaagt aatttcaccg cggaattgct 2580tgctttatat tccggttttc gttccccttt tcttggctcg ctggtgatct tcaagcattc 2640ttgaaatagg caccacacta cactacattc gtgctcattt gattgg 2686 4 2868 DNA Pinus radiata 4gaacacaatg cgggtctgat gtctctccgc gggtttgaag cctgacgagg ctgctcccta 60gggttgagtt ggaggttgct gcgggagcgg gaggcggggg cgaggctgat catgagatct 120ggattgtgaa tccgcgtgga tccgtggatg atttaacggg gcgaaagatc gagtctcgtc 180cccgagggct aagcaggcgg ttgaaataaa gaggtgaagt tattttgagc ggagtcatgc 240agcagccgta tgttgtactc gccctgttgt ggatgctgct gctgcatcac ccgctctggc 300gagtttttgc taatacagaa ggtgatgctt tacatagcct tcggtcgaac ttactggacc 360caaacaatgt gcttcagagt tgggatccaa ccctcgtcaa tccatgtaca tggtttcatg 420ttacatgcaa caatgataac agtgtgataa gagtggactt gggaaatgca caactttctg 480gttcattggt tccccagctt ggcctactga acaatctgca gtacttggag ctttatagca 540acaatataag tggtcccatc cccagtgatt taggcaactt gaccaatctt gttagcctgg 600atttatacct gaacaatttc actggtctaa tcccggagtc actgggcaaa ctgtccaggt 660tacgcttcct tcggcttaat aacaacagtc ttgtaggacg gatcccaatg tctctcacaa 720ccattactgc tcttcaagtc cttgatctgt caaacaataa tctcacagga gaggttccag 780caaatggttc attctctcta tttactccta tcagtttcgg tggcaatcaa tacctgtgtg 840gtccagtggc acaaaagcca tgtccgggat ctcctccttt ttctcctcct cctccgtttg 900taccaccccc gccagtagct ggaagcaatg gagcaagggt gcagagctct tccagcacag 960gagccattgc tgggggagta gctgcaggtg ctgccctctt atttgcagct cctgcaattg 1020ggttcgcctg gtggcgtcgc agaaagccac aggagcactt ctttgatgta cctgctgagg 1080aggatccaga agttcactta ggccaactta agaggttctc attacgggaa ttacaggttg 1140caactgatgg ttttagcaat agaaacattc ttggcagagg tggttttgga aaggtgtaca 1200aagggcgcct tgcagatggt tccttggtgg ctgtaaaacg tctgaaggaa gagcgtacac 1260cgggtggaga gttgcagttt caaacagaag tggagatgat aagcatggca gtacatagga 1320acctccttcg actacgtgga ttctgcatga cacccactga acggctgctt gtttatccct 1380acatggccaa tggaagtgtt gcttcatgcc tacgagagag ggcacaaaat gacccaccct 1440tagattggcc aactcgcaag cgcatagcat tgggttctgc aagaggtctc tcctacttgc 1500atgatcattg tgatcctaag attattcacc gggatgtcaa ggctgctaac atcttactgg 1560atgaagaata tgaggcagtg gtgggggatt ttggcttggc aaaacttatg gattataagg 1620acacacatgt tacgacggct gttcgtggaa ccattggcca catagcacct gagtaccttt 1680ctactggaaa gtcttcggaa aagacagacg tatttggata tggaatcatg ttgctggaac 1740ttattacggg acaacgggca tttgaccttg cacgtttagc aaatgatgat gatgtcatgt 1800tgcttgactg ggttaaaggc ctactaaaag agagaaggct tgatatgcta gttgatcctg 1860atcttaagaa caattatgtt gaagcagagg tggaacaact tattcaagtt gcattacttt 1920gtacacaagg gtcaccaatg gatagaccaa agatgtctga agtggtaagg atgttggaag 1980gggatggctt agctgagaga tgggaggaat ggcaaaaggt ggaagtcgta cggagccaag 2040aagttgaact tgttcctcat agaaattcag aatggattgt cgactcaaca gataatcttc 2100atgcagttga attgtctggt ccaagatgat aagagttgaa ttgtttattc aatctttgca 2160aatccataat tttattgagt ccaaattaca atgtcaatat gtatcgaggt tgaatcgatg 2220tatcattttc aagcatattt tagtagatgc gtcaatgtgc gaatgtagag aatattcgtt 2280gcaagtcctt agatggctat gctgggtttt gtcccatgag aatttctgtc gggttgcagt 2340caaacccgaa agttagaagg atttcttatg gtgcatccca tccaggcaaa gatctgggct 2400cctgtattat gaatggtttg ctctaggaat cgaagctcca tttctgctat tgaagacttc 2460ctttttcagt gctaatatgt atttaaacag tctgtccact tgacatatgc aactactttt 2520cctgccgaga gtgtactttg ataagaccac aatcttttga gactagtact aaatttggtc 2580tcagtttcaa atccagagtg gaaaatgaag gaaaacaaat ttcatgaata ccaagattct 2640gctaaatccc ttgagctggg ctcttaattc tcgataatcc tccggagttt gagaaagatg 2700tatgttgtca aaggtagatc tcaagctagt gaattcctta gtttcacaga attgttttct 2760tagagccaga cacatgctcc tgaattacat taaatttact ttttgtggtg ttcaaattga 2820aatacagaaa ctatgactgt tttaatgaca ttggcagcta acggaccg 2868 5 3340 DNA Pinus radiata 5gattaccata atgcttctcc tcgccactct ttctttcatt ctctttctca atccttttgc 60cttttccaca cccatcgccc atttccctca ccatcctcct cgtctcacca atgcttccga 120tcaacacgct ctgcttgcat tcaagtctgc aatcacatat gatccctcac agtccttggc 180cacgtcttgg ttgccaaatg tgtcattctg ccagtggacg ggcatcatat gcagcaggcg 240cagacagaga gttatttccc tcaatgtttc gagcatggga ttacaaggta caatctctcc 300ccttctcgcc aatctctcct ttcttacagt actcgacctt cacaacaaca gtttcgactg 360ccacattccc tatcaattgg gaaccctctt tcgcttaaag atgcttcgcc tgtctaagaa 420tcaactccag ggttccattc cgcccactct ggctaattgt cgcagtttac ggaacttgac 480tctctccttt aacaacctca cgggaaacat tcccccacaa ctatgccttc tccccaacct 540aatatgtatg tccttgggaa ttaacaattt gacgggaaca atcccggact gcttgggaaa 600catatcctct ttgcagtact tatctttgag tcaaggtaac cttcagggca gtgtcccttc 660cgaattgggt aggctaagcc agctcatcgt tcttgatctg tttgggaacc atctcacggg 720atgcattccg tcttcgttgt ccaactgcac caatcttgaa ctattggata taggtgataa 780ccaattagtt ggccacattc cgtcgcattt gtgtaccaag aagaccaccc agttaatgta 840tctccgtctg ggtgctaatc agctaagtgg cagcgttcct tcatccctct tcaattgtac 900caagttacaa gagattgcct taccatataa ccagctcagc ggaatagtgc ctatggagtt 960gggtaaattg acacatctcc agcggctttt ctttggcggg aattacttta ttagcggcaa 1020caccatgaga tgccccattc ttactgctct tagtaactgt tctgatctac aatatgtaga 1080cttatctgag aataacttca ctgggcaatt gccgttttct ataggccacc tctccaaaaa 1140actctaccat ttagacttgg gtagcaacga attagctgga gaaatacctc cggctattgg 1200aaatttaagt agcctaacat tccttaattt aggacgtaat tattttacag ggtcaatccc 1260atcttcactt attatgcttc agaagttgga gaggttatat atggattcca ataatttaca 1320aggaaacatt ccaatggaaa ttgggcagct aaagagctta ggtctcttat atctttctgg 1380aaataatttg tctggaaaaa tccctgattt tgtggccaac ctccagcaat taagatattt 1440atatcttaat cataaccagt tatcaggaga tataaatgca aatttaggga aatgtgtgaa 1500cttactactg ctagatctat catacaacaa gcttagtggg cacatacctc aagagctcgc 1560aggccttgca aatttagcct tctatttcaa cttgtcaaac aatttattaa gtggccatgt 1620acctttagaa ctaggaaagt tcgatatgct tcaagccata gatatttcgg caaatcaaat 1680aactggctac attccaagta tcgttggaag ctggaaagaa gtagcatatc tgaatctttc 1740ttacaatgca cttgaaggtc caattccagt gtcaatcagt gaacttctaa gtcttcaaga 1800cctagatctc tcctccaaca atttgtcggg tggaatacct atatcactag caaatctcac 1860aatgctccat cacttgaatt tttcttttaa caagttgtca ggggaagtcc ctaaagaagg 1920agttttcaaa aatattggcg ccacagcatt tatgggaaat cttggtctat gtggaccttg 1980ggtaaatcta ccaccgtgct atgctcataa acataaaagt gttttgaatc tcaaaagagt 2040tatcatactg gttgttgttg tagcaattgt tgtattgtgt ttgtttcttg caatattgtg 2100gagaaagaat tgtaggagaa atattcagag agacattggt ccatcattaa atgtggggca 2160tcgaagaatc tcctatgcag agctcgtcat tgcaacaaat gaatttagtg atgcaaactt 2220gttaggaatt ggtagttttg gaaaagtgta caaagggatt ttgaatgatg gcacaatggt 2280tgctgtcaag cttctcaatc tacaaaatga aggggctcaa aagagttttg atagagaatg 2340caaagttttg ggtagagtta gacaccggaa tttgattcgg gtcataactt gctattcaga 2400tctccaaatc aaagctttga tatttccatt aatgccaaaa ggaagcctag aaaagtggtt 2460atatcccgat gatggagaac aaagttgttt gaatttgatt caaaggttga atatagcaat 2520agatatcgcc caaggcatga catatctcca tcatcattgc tttgtgcaag tgattcattg 2580tgatttgaaa cccaacaatg tgttgttggg tgaagatatg actgcatatt taatagactt 2640tggcattgct actatatgtt ttgcaaataa tgaagatgga gctctcactt ccacaaatgc 2700actcaaagga tctaccgggt acattcctcc aggtattatt taaactatat cccatatgaa 2760gcaatcttat gattttctta tgtttactta acattttcaa ttaaattaaa aaacttaaga 2820tgaggatcta tgaatgcaga atatggagtg ggaggacaag ttacaaccaa aggtgatgtt 2880tatagctatg gaatagtgct attggaaatg cttactagaa agaagcccac ccatgacata 2940tttgtggaag gaatgaactt gcaaaaatgg gtgggtagta gttttccaag ccaagtacaa 3000gaagtggtgg acatgggtct gttaacgagg actagtagat gcattgaaga agataaagat 3060ctcaattgtc tcagtgcact gataaatgtg ggtttgcttt gcacaaatga atctcctcaa 3120ggacgaccta caatgatggg cattttaggc acattgcaaa acatcaaaga ctcttttttg 3180agcactactt ccattcccaa attccaatca aatctaacac atttgttggg tagtacaagt 3240gctactatca acaacatttg tgaaggtcaa agttcttcca catcttagtc tattgtaaca 3300aagtagtggt tatatgcttt tttgtacata aaaaaaaaaa 3340 6 3396 DNA Pinus radiata 6ggaggaagcc tgagacgaaa tcattgcagg aggcggatgc ccaacaaata ggttgatgca 60gggattgcac gcattcttca tttatttgct gtgaaaaatg aggtattcct ggacatgttc 120tccctttctc agtctcctgt tcattctttc atgtttagat tctggaattt gtctcgataa 180ccaacaaaca caggtgatga aaaaactgtc gtcctacaca ccctcatgga cgactgtaaa 240gtctgataac ccatgtggat ggagtggtgt gaattgcact gccgaagaga gtaatgtgac 300tgagcttcat atgtccggtt ttaggatgaa gggcaatgct tggcagacta tatgcaaact 360tcaagccctt caagtgcttg acgtctctga taatttgctg tcaaccccgt ctgataatga 420tatccaggct tgcaccaact tgttttccct taacatcagc tccaattttt tgccaggctc 480ctcccttcct tcgcttgcac cgatgcgcaa actgcacttc cttgatgtct ctcacaatgg 540atttgcaggg gaatttggcc cgcagattca gcatttgaca gacctcagag tgctcaattt 600gacatacaat aacttcagtg gtccaatccc ttcattttta ggaaatctga cgactttaga 660aaaaatcgat ttctctcaaa attattttga gggtgaattt cccaaagagc ttgtgcgttg 720cacaagtctg acttatctag acctcagctt caatagactg acaggccaaa tccctgataa 780tattagcaac cttatccatt tagaaaccct gattctgtca tcaaataatt taactggaac 840gattcctaaa acactggatc gacttgtcaa tttgactcac tttgcatcaa ataagaatca 900gctcattgga cgaatacctg ttcagttagc taagctcaca gaattacatt ttcttgatct 960gagctacaat gggttaaatg aaaccattcc tccagaactt tttgctttga gcaacttgca 1020gactctggac cttacaaaga atttgctcac aggagagatt cctcagaatt tttcgagaaa 1080attgataagg ttgcgaatag gccagaatct tcttaaagga aacattcctt taacaattgg 1140aaattggtct aacttgacat acttagaaat gaatgataac tcactagatg ggcagattcc 1200gcagcagctg gtaaattgta tcaagcttca actacttgac ctcggcaaca ataatttgtc 1260aggctcatta accaatcagc tgccttcact tctacagttg caagtgttga agttgcataa 1320taataatttt gttggaagca ttccatatat actgtcatct ttctctaatt tgtcatatgt 1380cgatttgagt gacaatactt taaatgggtc cataccctca aatattttca atctttcgaa 1440gctccaaaat ttgcgcctac agaacaacaa gctaacagga gcgattccaa acactgttgg 1500aggcagccag gtcttgttag agctccagtt gggtggcaac aacctaactg gaacaatgcc 1560attggaaatt ggcttcgtca gaaagttgca aattcaactc aatctcagct gcaattctct 1620agaaggggaa attccgaata ctctttctgg tttgtacatg ctagaaatct tggatctttc 1680caacaataaa ttgacaggag aagttccagg ttctttgaca gctatgctca gtctcacact 1740gttgaacatc tcaaacaata gtttaacagg cgtccttcca aaatttccaa attccacaag 1800cgcgttgata attattgata caggaaaccc tggtctcact gctgggcaaa atggttccgc 1860tcctgccgct tctgcaagga aaaaaatctc ggcaattttg atcattggag ttgctgttgc 1920aggtgctgtc tttgcaattg tggctgttgg attgttcatt gtggccagca aatactttgg 1980gcgtggtgat cagcaaatgc cagaagtgca actagcccgt aagattgaag gccattttat 2040tcatccagat agtatccaca ggctaaggat tgactttgaa aagggtgttg aagccactct 2100agatccggct aatgtcttcc taaagaacaa gtttagtacc tattacaaag ctgtcatgcc 2160ttctggcatt agttattccg tgaagaagct caactggagt gacagaattt tcaaatctgg 2220aagctaccga aagcttggtg cagaactgga gaaacagggg aagctaaggc atcccaacat 2280cctgacaccc ttggcacatg ttctagacac agattctgct taccttttct acgaatatgt 2340tcataaaggc agcctctcag aattccttca cactagtaat gtttctgtat tggattggcc 2400atctcgttgc agaattgcca taggtgtagc gcaaggtttg gcttttctac acgggtgtca 2460gcatccaatc ttccatcttg atctcactac caaaaacatt ctcctaaagt ctttaacaga 2520gccacaaata ggtgatattg agctttgcaa gattgttgat ccttccaaga gcacagggag 2580tatttctgca attgctggtt cagttggata cgttcctcca gaatatgcat acactatgag 2640agtgacggct gctggtaatg tctatagctt tggcgtcatt ttgctagagt tactgactgg 2700aaggactccc atcaccagtg gaatggatct tgcaaagtgg gttcagtcaa ctctctcagg 2760ggaggaaaca tgggaacaaa ttcttgacac aggaatcaga aacttttccg tccaaataca 2820gaatgagatg atcgcaatgc tgaaagtagc tctcagttgc gttagctcat ccccagagtc 2880gagacccaaa atgagaaatg tagtgggaat gctccagatg gtcaggcaag ttgcagaata 2940aggttttact tcattcgaca atatatggat gagattttac ttccctacaa tataggagtg 3000ggaacggggt attctgtttt atatttttaa ggagactaaa tctgtatagt attgtcgata 3060tgattaggat tcacatgaat tcagacatgg atatatgagg ttgttaaaat gttggtattt 3120tttgctcagg ttagcgtagt tatgcagtaa agtgcttatg ctagattatc atggttcata 3180tcataaatct agtatacttc caagcacaaa tgtataaata tatgtgtttc tgatttgatt 3240aacggggaag ttattgtctt cattggcatt caaccaaaat atcagttaaa ggtagcaagt 3300ttatagaatg cttgtggctt tcaaacatgg tcctggcttg tggctaaatc atggtaattc 3360aagaacacag tctatttgac taaaaaaaaa aaaaaa 3396 7 2432 DNA Pinus radiata 7tctcaaagct ctcaatccca ccactattgg acaagaatgg cagaagtgaa gaaaattgtt 60gagagtttgt cgcattgcac tgcacaatgt gtttcttgat gctctaaagt cccgctcatg 120tgagttattt tcctgatacc ttatctgtac ttgatgttac ctgatgcatt tcaagatctt 180gtttgatgca aaggaggaaa aaattaagtc ctctttcgat atgttgactg cctttctggt 240attgtgcttc actaccactg ctgcgtctgc tacacaacta tctccttcgg gcctcaacta 300tgaagttgca gctctaatgg cgataaagaa tagtttgaat gatccacata atgtcttaga 360gaattgggac atcaattcgg tggacccatg cggttggagg atggtgacat gcacacttga 420aggctctgtt tctatattag gaattcagtg tcagaatttg tctggtagtt tgtctccaag 480cattcgaaac ctcaccaatc ttcagtctgt gctacttcaa aataacgcca tatcaggatc 540aatcccagct gagttgggaa agctagataa gcttgatact cttgacctct ccaacaatca 600tttcaatggg ttgataccca gttcactggg aaaattgaaa aacctaaatt acttgcggct 660caacaacaac aacctatctg gaccaattcc cccatcactg gctactatca cagggcttac 720tctcctggat ttgtcgtgca ataatctaag cggctctgtg ccaagaattt ctgctagaac 780cttcaatatt gtgggcaacc ctttgatttg tggtccgaat tcaacatata agtgtcctgg 840acaatttccc acgcctatcc cattagttgt tgagacaccg caaggtagag ttccttcaag 900acaatctaaa accagaaagc tggctgttgc cttggtcgct agtcttggtt ttgtgtttgt 960ggtttccatt ggacttctcc tttggtggcg caagagacac aatcagcaaa ttttcattga 1020tgtcaatgaa cagcacaatg ttgatatttg tctagggcat ttgaagagat tctcattcaa 1080agagttacgt gtttccacta ataattttag tagcaagaat attttaggag taggaggata 1140tggaattgtc tataaaggat tcctacaaga tggcactata gtagcaataa aaaggttgaa 1200agatggtaat gtgggaggag gagaaattca atttcaaaca gaggtggaaa tgatcagctt 1260ggctgtgcat aggaacctat tacgattgta tggattttgc acaacctcca gagagaggct 1320tctggtctat ccctacatgc caaatggaag tgtggcctct tgtcttagag atcatattaa 1380tggaaagctt gccctggact ggcctactcg caagcgtata gcccttggag cagctagggg 1440actgttatat ttgcatgagc aatgtgatcc caagattatt caccgggatg tgaaagcagc 1500aaatatatta ctggatgaat attttgaagc tgttgttgga gattttgggt tagcaaagct 1560cttggatcac agggattctc atgtgactac tgctgttcga gggacggtag gtcacattgc 1620cccagaatac ctttcaacgg gacaatcttc agagaaaact gatgtatttg gctttgggat 1680attactgttg gaactcatta caggacaaag ggctttagat tttggccagg ctgcaaagca 1740aaaagttgta atgctggatt gggtaaagaa gcttcatcaa gagaagaagt tgcacctcct 1800tgctgataaa gatcttaagg gcaattttga tagagttgag ctagaagaga tggttcaggt 1860ttctttgcta tgcacccaat ttcagcctgg acatcgtcca aaaatgtgtg atgttttgag 1920aatgttggaa ggtgatggat tgacagaacg gtgggaaaca ttgcaaaaaa ttgaaacccc 1980ccgatacaga gtaactgaga tacccataac atattccgag ttggttgaag aagattcttc 2040ttggcttgtc caggcaattg agctatctgg tccccgttga tttcacaggc ttgctaacat 2100tctaatatca atgcttccat agcttgttgt ctttgcatct tttgttggtg cccatggaaa 2160catatgctgg ttcgttgtat aggtagcttt ggtacagtca aaatatttcg ccatgcacca 2220aaaaatacgg atacctttat aattgtctct ctatgtaatc tcaggcaaac ttaagcatat 2280tcagtatttt ttattgataa gttggtagct ttgcttgtaa tggctatgct gtatcctcgc 2340ttgacacttc atcagcagcc tagcttattt gatagtgaag ggttttcaca agaactttct 2400cagatggttg taaatggttg ttgagatatt tc 2432 8 2638 DNA Eucalyptus grandis 8cgaggaccaa ccgccaagaa ccatgcattt acactctctt ttactccatt cataacctcc 60gctccccagg gcagagcaat ccaagaatac ccatctcatt cttttgcagc tgctgcattt 120cttgatctcc ccgccacaat gcatttccga attctagccc tcctgggctt cttcttcttg 180gccgtcctct tcccctccgc ggagccagat ctcgcgtccg accgcgccgc cctcctcgcg 240ctgcgatccg ccgtcggcgg ccgcaccctg ctctggaacg ccaacctccc gagcccctgc 300tcgtgggcgg gcgtccagtg cgaggggaac cgggtcaccg ccctccgcct ccccggcgtc 360gccctgtccg gccaaatccc cgacggcgtc ctgggcaact tgacccagct ccgcaccctc 420tcgctccggt tcaacgcgct ctccggcacg ctcccctcag atctcgcgtc ctgcgccgac 480ctgaggaacc tctacgtgca aggtaacctg ttctctggcc cgatccctgc gtcgctgttc 540ggcttgagtg atctcgtgag gctcaatctc gcgtccaata agttctcggg ggactttccc 600gctggtttcg gcaacttgac taggctcaag actctcttgc ttgagaacaa ccagctctct 660gggtcgatcc cagctgacct gaagcaactc aagctcgagc agttcaatgt ttctaacaac 720ttgttaaatg ggtcgatacc tgagggcttg ggggcttttg ctacctcttc gttttccggt 780aactctctgt gcgggaagcc gctggcttct tgctctcaag atattgctct gcctgccggc 840gagccttctg gtagtccagg gcagccagga ggaaagaaaa agaagctttc gggggctgtg 900gtcgcgggca ttgtgattgg gtgcgtgttc gggttcatat tcctcgtcat actgttgatc 960tatctgtgcc ggaagaaggg tagcaagaag tctagatctg tggacgtcgc gaccttcaag 1020caccaggaat tggagattcc aggtgagaaa ccggtcgggg aggtcgagaa tggcggtttc 1080agtaatgggt actccgtggc ggcagctgcg gctgcggcca tgacaggaag tggaaagggg 1140gaagtgaatg ggagtgctgg tgccgcggca aagaagttga tcttcttcgg taattctgca 1200agggcgtttg atttggagga cttgttgagg gcttcggcgg aagttctcgg gaaagggacc 1260ttcggaacgg cttacaaggc ggttttggag gccgggatca cggtggccgt gaagaggcta 1320aaggatgtca acgtggctgc aaaggaattc aaggagaaga tcgaagcagt cggggcaatg 1380gatcaccaga gtctggttcc tctgagggca tactattaca gcaatgatga gaagcttctt 1440gtctatgatt atatgcctat gggaagcttg tctgcacttt tacatggaaa caaaggagca 1500ggaaggactc cgctgaactg ggaaatcagg tctgccattg cgcttggggc tgcccgtggc 1560atagagtacc tacattccca aggtccaatc gtctcccatg gaaatatcaa atcctccaac 1620atccttctca cgacatcata tgatgcgcga gtgtcagact tcggcctagc ccaccttgtg 1680gggccttcct ccactccaaa ccacgtcgcc ggctaccgag caccagaagt caccgaccct 1740cgtaaagttt cccaaaaggc cgatgtctat agctttgggg tgttgcttct ggagctcctg 1800actgggaagg cacctattca ctctcaactc aacgaggaag gtgtggacct tcctaggtgg 1860gtccagtcca ttgttcgcga ggagtggact tccgaagtct tcgatctcga gctccttagg 1920taccagaaca tcgaggagga gatggttcag ttattgcagc tcgcgataga ttgtgccgcc 1980caatatcctg ataagcgtcc ttcaatgtcc gaagtgagga gccaaatcga agagctatgt 2040cattctagct cgcagaaaga tcgtgccccg cagcttgacc aggtcaatga agtgaacgat 2100gacacgtctt ctcggtgagt tgatttccga gattggcgag cgaaattccc gaaggatcat 2160cttgcaacat catgttcaca tttagcgtca taagctgggt tttcttgtct ttttttctgt 2220tctatgtcca tttgggcctc tttctaatgt cattacgagg caaatagtct tctccccttt 2280ttcccctcat cattttctgt aattccccat tgtgctacat tttttcatct tttctttcat 2340ttatgctttt tggggtggat atcagttact ttgttcttgt tggtaagtat gttggatcat 2400ggtttcttgt aattattact catgcttttg cttttgcttt ccacttttgc ttttgatgca 2460acttctcatc tgttcagact gagatgtttc agttgcttct taggatgctt ggtgtaattg 2520tgttttcaag tgtaccgatc caccaaactt tgtttaaagt ttcctggacc ttttgaaaaa 2580tcagattgag atgtatacct gaagtctaag tttattttgt gaaaaaaaaa aaaaaaaa 2638 9 3239 DNA Eucalyptus grandis 9gcaacacccc ccctcctgct gaattttcaa cacatcaaca tcatattcac gttctcatta 60tgctctgcaa gtcgtcgcgg aggaccggga caatagcagc aggaggagcc ttggaggcaa 120gacgagttct ttgacttgtt tttctccttg gcaagggaag gtatggagga ggccttcctg 180aggttgatct tcctggtggc ggtgcttttg tttgggaagg atctgcagct ggttttttct 240ttcaccaatc ccgatgactc cgtggctctc caatccctca agatgtcatg gcagaacacg 300ccgcctagct gggaaaggtc gagtgacccg tgtggactcc cttgggaagg agtaacctgc 360aatagcaatt cgagggtaac ttcattggga ttatcaacca tgggaatcaa gggccagcta 420attagtgaaa tagcaggact cgctgaacta agatctctgg acctttcctt caacaaagag 480ctcactggtc cgctcgctcg gcagttgggc aatctacaga agttgaacat actgatttta 540gccggctgta gtttcaccgg tagtattccg gacgaactgg gcaaccttgc agagctatca 600ttcttggcgc tgaactcgaa caacctcaca ggcaatatac ctgcttcatt gggtaacctc 660tccaagcttt actggtttga cctggctgat aatcaattaa cggggcctat tccgatatcg 720acggacacat cccctgggtt ggacctcctg ttaaaagcaa agcactttca tttcaacaag 780aacaagcttt cagggcccat tccggaaaag ctattcaact cggcgatggt tctaatacac 840gtcttattcg atggaaatca gcttaatggt tctataccgt cctcagtagg acttctaccg 900gatcttgagg ttcttcgact tgacaggaac aagttatctg gaaaggtccc tctgaatctc 960aacaacctga caaaccttag tgaactgaac tttgcccata atgcattgac tggcccttta 1020ccagacttga cggatatgaa ttccctcaat tacgttgacc ttagcaacaa cttctttgac 1080ccttcggaag ctccagattg gttctcaact ttacctactc taaccacgct ggttatagaa 1140tatggaccgc ttaagggggt tgtcccacaa aagcttttca gctttcctca gctacagcaa 1200gtgaaattga agaacaatga attcaatggt acgctgaaca tgggagacaa catcagtccc 1260cagttgcagc ttgtcgatct gcagaacaac caaatatcat cggtgacgct gggctcttcg 1320ggttactcaa atacactgat gttaataggc aaccccgttt gcactaccga actctcgaac 1380actaattact gccagctcca gcagcagaca gtgaagcctt attcgaccag cctcgccagc 1440tgcggaagca agtcgtgccc tcccgacgaa aggctcaacc ctcagagctg cgagtgcgca 1500tttccatacg aagggacctt atattttaga ggtccctcct tcagggaatt gtccaatgtg 1560accttgtttc acatgctcga aatggacttg tggacgaagt tgaatctcac tcccggttcg 1620gtttctcttc agaacccctt cttcaatctt gacgactacc ttcaagtgca gctttcactc 1680ttccccccga gcgggaaata tttcagtcgg tcggatattc agagcatcgg tttcgacttg 1740acaaaccaaa ctttcaagcc tcccaaacca ttcggcccct attacttcat cgcctccccc 1800tatgcttttc cagacaatgg aggaaccgcc ataagcaaag gtgtgatagt tgggatcgct 1860attggcggca cggttctggt tcttggcctt gttgtattag ggttatatgc tattcgacaa 1920aagaaacggg cggagaaagc tctcgagttg agcagaccct tcgcatcctg ggcacccagt 1980gggaaagata gcggaggagc gccacaactg aaaggagcac gatggttctc ctatgatgaa 2040cttaagaggt gcaccaataa tttctccgat agcaatgaat taggcttcgg aggatacgga 2100aaggtgtaca ggggagttct tcctgatggt catatattag caatcaaaag agctcagcag 2160gggtcgatgc agggtgcaac cgagttcaag acagaaatcg agctgctttc gcgggttcat 2220cacaagaatc ttgttggcct cataggattc tgtttcgagc aaggagagca gatgttggtc 2280tatgaatata tgcctaacgg gacgctcagg gatagcttga caggaaaatc aggcatttat 2340cttgattgga agaggaggct tcgtatagct ctaggttcgg ctagaggact agcttatctg 2400cacgaactcg cgaatcctcc aattatccac agagatgtca agtccaccaa tatcttgttg 2460gacgaacatc tgacggccaa agtcgcggat ttcggtttgt ccaaactggt atcggacagc 2520gggaaggggc acgtttcgac gcaagtgaaa ggcacgctgg gctatttgga tcccgaatac 2580tacatgagtc aacagctgac agaaaagagc gatgtgtaca gcttcggggt ggtcatgctt 2640gagctcatca ctgcaaagca accgattgag aagggcaagt atgtcgtccg cgagattcgc 2700accgccatgg acaagaacga ccaggactac tacggcgtga gggaaatgat ggacccgtcc 2760atgaggagca tgggctacct cgtcgggttc agcaggttct tggatttggc gatgcgatgt 2820gtcgaggagt cggctgcgga ccgccccaca atgagcgagg tggtgaaggc gatcgagacc 2880atgttgcaga acgatgggat acacaccaac tcgacgtccg catcgtcgtc ggcgacggac 2940tttgggtcga cgaagggcgc tcctcggcat ccgtacaacg atgccttacc caagaaggaa 3000gttagctata gcgattcctt tgattatagt ggtggatatg gactatctac aaaaattgaa 3060cccaagtgaa aaacatgatc aattgatctg ctcagtcact tgtttttctt tttcctttgt 3120ttctgctcag gtttggcttg gtcctcttca caatttctgc aagtgagtaa cctgtcttac 3180atgtgtaata gttttgatca gccgcatcaa gtgaaggaag ttcttgtgca aaaaaaaaa 3239 10 2336 DNA Pinus radiata 10attcgattcg cgatgctatt tccatggcgc agcctcgttc tgatagcctt cacctccctc 60gtcgtacaac ttattcctgc gcaggccgtg gaggatcgtc gacatgacac cactttcctg 120ttcgacggat tcaatggaac caacttgatc ttggaggcta atgcttcagt catcggctct 180gaatccgtac tctcccttac aaatcactcg catgagttca tgctcgggcg cgcgctgtat 240gcagctcctg tgcaaatgaa aaacaaccat acggtttcct ccttcagcac taccttcgtt 300ttctccattg tgcctcctcc ctctaacgaa gggggacacg gattagcctt catcatgacg 360ccttacacat ctccgatggg cgctcagccg gtccagtatt tgggcttgct taacctcacc 420agtaacgggc agccatacaa tcatttattc gcagtggagt ttgataccat catgaatgta 480gaattcaaag atccagaccg caaccacgtg ggcgtggata tcaacagtct catctcagtc 540cagacagaga ccgccggcta ttggaatggt gaggaattcc atgaactcaa cctcaggagt 600ggacggaaca tacaggcctg gatcgattac gatcatcttg agagcagtct caatgttacc 660attacggtgg ccgggttgcc gagaccgcag aggccgttga tatctctgca aatcgatctg 720cagaatattg tagaagagaa gatgttggtg ggcttttcag cggcaacggg gctgttagtg 780gaagaccatt acattctcgc gtggagcttc accaccgaag acacggcgcc gcctctcgat 840gtttcttgcc tctcttcatt tgccaacatg tattccgaac ctctgagccg gggattcata 900gccggtgtca ccgtggtttc tgtggttctt ttctggctgg tgattgcggc ggccatgttt 960ctgagaagaa cactaaacag ggaaacggtc gaagaatggg agcaggagta ctggccccat 1020agattcgatt acaaggagct gcgtatcgcc acgcgagggt ttcgggacga aaaccttttg 1080gggtacgggg gatttggcat ggtttacaag ggttttctcc ccaggagcgg ccaagaagtc 1140gcagtgaaat gtataacgac ggagttcaag gaaggaataa aggggtttgt tgcagagatc 1200tcaagcatgg ggcggctaca gcaccggaac ctggttcaac tccgaggatg gtgccgaagg 1260catacacagc tattcatcgt ttacgactac atgcccaacg gaagcctgca taaactcatc 1320ttcggtagtc cgacaacagt cctgccgtgg catcggcgat acgcgatcct aaagggcgta 1380gcagcggggc tgctgtatct gcacgagcaa tgggagaaga gggtcgtcca cagggacatt 1440aagtcgagca acgtgctgtt ggattcggag ttcaacgggc ggcttagtga cttcgggctc 1500gctcggctgt atgatcacag tgagaatccg gagacgacat atgtggtagg aacgttgggg 1560tacatagcac cggagttgat acaaacgggg aaggcaactc ctagctcgga cgtgttcagc 1620ttcggtgttc tgctgttgga ggtggcttgc gggaagagtc cagtggattc gttggaggac 1680tctgagcgca tgattttagt ggagtgggcg tgggagctct acacggaggg gaggttgctg 1740gaggcgtcgg atccgaagct ggccgcaaag ggtggatatg atgaaggcga gatggagaag 1800gtgttgaaat tggggttact gtgttctcat ccagagccgg agagcaggtt gagcatgcgc 1860caggtttgcc aagtactgaa cggcgaagct ccagttcctt gtagatggta gggacggggc 1920aattccggag gtcaggatta tccatgtttg attaaagaat ataatacttt tatttattat 1980atattatttt atgttcccat gcgggcccag cgaggacact gctattgttg ctggtcagtg 2040gttcaaatcc gggaaagaga gcatgccgtt actcaagtta ttgctagcta tgcgggggat 2100ttaagtagta gtctactgtt acaaaacata gatctgccct atctacttcc tgtgacaaag 2160cagagttgaa tgctcagaga gcaggaattt gaaatccaaa ttcctttgta ttaaacattt 2220tagttaattt tatgatgcgt aggcttcatt tgtcaccgta ttcgattcgt aaagggcgac 2280actaacaata attttataat atttgtaaaa tttttggtgt taaaaaaaaa aaaaaa 2336 11 3097 DNA Eucalyptus grandis 11tttgttctct tgcaatgcca tatatacacc tgaaattctg atcgctctca ctcatctgtc 60gcattcaaag cctcaaagcc gcttgtttct tgaactttgc cttggcttca aagaagaaag 120tcctcaaata gaagatcgac catatgggac tgaagatatt ctcagtcggc tttgctcttc 180tttgttgctt ctgttcactt ggcttctgtg atcaagacgg ttttctgagt ttagcttgtg 240gtggaactac caattacacg gattcatcca acatctggtg gattaccgac agtgatttca 300taagcacagg aaagactacc tatgttgaca atatcgaggg caattcatct ggtgtttcgc 360ttcggttctt cccagattcc aaagtccata actgttacag attgcctgtg aggaatatat 420cctccttaat tctcgtaaga agccaatttg tgtacaagaa ctacgatgga cgagggaagc 480caccggcatt ttctgtttct cttgggacag caatggctag tactattaac ctgaccacta 540atgatccttg gactgaagaa ttcatatggc cggtcgacaa ggacacactc tctttttgct 600tgcatcgtat tcggaacggt gggactccag taatttcttt gctcgaagtg cggcctcttc 660ctcctgaagc ttacaaaagt ggaatggggg attacccgaa taagttactc aggaagtctt 720atcgaattga tagcggttac accaatggct cgttgaagta ccctgcagat ccctatgatc 780gaatatggga tgctgataag agcttcacac cattccatgt gactactggg ttcaaaattc 840aagtcgagtt taatctctcg gggctctcgg agagtcctcc tcccgctgtt cttcaaactg 900ccagagtttt ggcacgaaaa gaagtcctga cgtataactt ccctctcgac tcacttggcg 960actattacgt cgtcctctat ttcgctggga tccttcctat ttccccatct tttgatgtga 1020taattaacgg ggacatagtg caatctaact atacagtgaa aacctcagca gccagtgctc 1080tatatgttac gaggaagaaa attaagtcgc tgaacgtgac gttgaagagc aaacgtttct 1140ttcctcaggt taatgcaatt gaggtgtatg aacttgttga cattccccca gaagcttcat 1200caaccacagt ttcagcactt caggttatcg agcagtttac tggcctggac ctgggatggg 1260aagatgatcc ttgctcacca aaaccgtggg atcatgtcgg atgtgaagga agcctagtaa 1320catcactgga tctttcagac atcaatctga ggtctatcag tccaacattt ggggacttgt 1380tggatctcaa aacactggat ttgcataacg cgtcacttgc tggtgagata cagaacttgg 1440acagcctgca aaatctcgag aaattgaacc tgagtttcaa taaactgaca tcgtttggct 1500ctgattggga gaacttgatt agcctgcaag ttctggacgt ccaaaacaac agcttagacg 1560gagttgttcc cgacggcttg ggagagctga aagaccttca cctactggac ttggagaata 1620acctgctaca aggtacctta ccagattcct tgaacagaca gagcttggag gtcagaacct 1680caggaaactt gtgtctttcc ttctccacaa ccgcgtgcgg tgatgcatca tctagtcctt 1740caattgaggc accgcaagtt acaatagttc ccgagagaaa caaaggggga cataatcgtt 1800tagccattat actcggagca gtcggaggag tatcactagc tattttactc atcccgctct 1860tcgtattcat gtacagaagg agaggaagaa ctgaaatgtc atatacggaa agggcagtcg 1920cagacgtgag aaactggaat gcagctaaga ttttttccta caaagagatc aaaacagcta 1980caaacaactt taaagaagtc attggtcatg gaagttttgg atccgtgtac ctgggaaacc 2040ttccagttgg aaaactagtt gctgtgaaag tgcggtttga taaaacccaa cttggtgcag 2100attctttcat aaatgaggtt cgtctcttat cacaagtccg ccatcagaac cttgtcagtc 2160tggaaggatt ttgttatgag tcgcagcgtc agattttagt ctatgaatat ctaccgggtg 2220gatcactggc tgatcaactg tatggtccaa acagtaggaa attctcacta agctgggttc 2280gtagactcaa gattgctgtt gatgctgcaa aaggactgga ctatctacat aatggaagca 2340atcctcgaat catacaccga gacatcaagt gcagtaatat actattggac aaggagatga 2400atgcaagact ttgcgacttt gggctctcta agcaaatgat ccagccagac gcaactcacg 2460tgaccactgt cgtcaagggc acagctggtt acctcgaccc tgaatattac tccacccaac 2520aacttacaga gaaaagcgac gtctatagct ttggagttgt gcttttggag ctcatctgtg 2580gacgagagcc gttaaatcat tcaggaactc cagattcctt caatttggtt ttatgggcaa 2640agccctactt gcaggcaggt gcatttgaga tagtggatga gagtttaggg ggaagtttcg 2700atgtggaaag catgaggaaa gtggcaaaaa tcgctgtgag gtctgtagag agggatgcat 2760cactaaggcc aaccattgca cagatactgt ctgtgctcaa agaggcttac agcattcagc 2820tctcttatct tgcagcctct ggacatgtga actgaatcta ctcgtattac aaccaagaca 2880agattttttt cttttcggcc caaagagata aaaagaggtc gtttgtcctg ataacaaggc 2940tgtttatcat tcgtttgagt ggaacctatg tacagagaga gttttgggtg caattatcat 3000atttatcgta cttaaatgta tgatactgtt ttatttcata ttcatttgtg caagagaaat 3060taagcaagtt tttgttccta aaaaaaaaaa aaaaaaa 3097 12 2649 DNA Pinus radiata 12gttggaagac cctgtaagca tttggcttcc atattgctat ttggcaaaga gagaaacatg 60ggttgtcgga aagctgtgag atctgtgagc ccacagaacc catcttcctc tctgatgcat 120ctcagactgt taggtgtttt gctcatgctt cattgcattg atgggctgat cctaaatcct 180gaagtttcta ctcttctcac actgaaacca acgttgtctg gaacaggggt taacgttgta 240ctggcctcgt ggagtgcctc tcgccctctg tgtggatgga aaggagttat gtggatgtac 300aatggtatac ctgtcaactg ttctgttccc aaatttaggc acagtgtggc tttggcttac 360agtcgcaagg tctcagtgct ggggattgat ttggaagcaa cagggttgaa gggtacagtc 420ccaaaggagt tgggctcctt tatatatctg cagcagctga atctgaataa caatagtctt 480acaggaggga tgcctccaga gttgggaaat gtgcctaatt tgagcagtct gcaactcaag 540aacaatgggc tgaatggagg cattcctact gctatttgga atctgtgcga taacattact 600gagctggaat tgggattcaa tgagctatca gggagcatcc ctgaacccgg aaatgggtct 660aatataggtt gcccacaact ccggagattt gaggtgaata acaacagtct aacaggtaca 720atccctagct tccttgctaa ttgtatttct cttcaagagc ttgatttgag tgggaattct 780ttcaccggcg aaatacccaa tgagcttgca aatctgccaa acctgacaac tctcaacctt 840gcccacaaca atctaagtgg tcgtataccg agttttcggc agaaatttga taagaattcc 900tttgtagaaa atagtggatc cctgtgtggg cagcctctgt taaacccatg tggcgtagct 960ccaaatgctg ctagtgctgc ttctgcaaat gtgactgcag cccagttcaa tgtgaccaaa 1020gcccatttca aatctatgag cactggagct attgctggaa tcatcattgg atcaattgct 1080gttgtagtca ttgccagttc tttgttaatt ggatgctacc ataggttcag cacagatgcc 1140actgacaagt cttcgtcttc gtctgcccct tcgaagaaag ataaggaaga agacatggac 1200aatgtgagtg ggaaattaat aaacttccaa ggaggtgagc atttgaccgt ggatgatgtt 1260ctgaatgcaa cgggagaagt cttgggcaaa tccagttatg gaactgtcta caaggccagg 1320ttaagtagtg gttgtatgat cgcactccgt cttctccgag atgggtgtct cagaagcaca 1380gacgaattca tgcctgccat tcaggagttg ggtaccatca ggcataagca tcttgtttct 1440ctaagagcat tttattcagg gacaagaggc gagaaactgc ttgcctatga ttaccttccc 1500agaggaagcc tcgcagaatt gctacacagt acaaacaggc ctgctcctgg atgggctaga 1560agacataaaa ttgctctggg agctgctaga ggtcttgcct accttcacac tggattccac 1620aaatccatca tacacggcaa tatcaagtca aaaaacatac ttgtggatga caattatgtt 1680gctcatcttt ctgactatgg tcttaacaag ctaatgaact caacagcaaa tattgagatg 1740ttagaagctg ctgcttccca gggatacaaa gcacctgaac ttatcaagat gaaaagggcc 1800aatgcaaaga ctgatatata cagctttggt attggtttac ttgaaattct cacaggcaga 1860agacctggta gaacaagctc atccaatcat attgttgatt tgccaacagt cgtgaagaat 1920gcagtgcttg aagagaggat ttcagaactc tttgaccttg agcttttgcg tgcaatgaga 1980agtccagcag atgaaggtct tctgcaagtt ctgcagcttg caatgggttg ttgtgctcct 2040tctccatcag tcaggccaca tataaaagag gtagtccgtc aacttgagga gattaggcca 2100aaacctcaat ctccgcatct tgccctttcc ccacaataca attcagatga caagagtagt 2160agggagtttt tgttggatgg cggacaaaat taaagatgga atttactcgt ctttcctccc 2220tagttccgac acttatttag gttctacata tgctataaac tactaccgta gttacagata 2280cgggaacata ttaagggatc tatttttctt ttcttgcaag atatgtctca gattataatt 2340tatgcccatt gggaggggct ttcttttgtc agatcatttg gtgttctgag aggctgtaag 2400agaaacatca atttagtccc ctgcttctgt acgatttcct atttgtaaat catactgata 2460gttgtgagga ctctcagttt ctgtttgtgt ttttcatacc agagtagatt tccttttgtt 2520gtcaaagtca tgacatagta aaaatcatga caaaaattga catgtagacg tgtaaactta 2580gtggtaaaga tatgcttata tgtatatgaa ggatatgcac ttgcgtttaa aaaaaaaaaa 2640aaaaaaaaa 2649 13 2513 DNA Pinus radiata 13gaaatgaaga tttgagttct ccggaaacac actaaggata tcgtagctct ttgaaaatgg 60gaaagaaaat tttgcaatgt ttgcggttaa ttattgctat attgacggcg tctattgcga 120tatcgcacgg aactacggat ccagacgacg tttctgcgct caaaggcata tatagcagtc 180taaattctcc ccagcaactt tctggatgga gtgcaaatgg aggagatcca tgtggtcagt 240cctggaaagg ggtttcatgt tctggatcat cagttacatt aatcaagctc tcaggcttag 300gattgtcagg atcattatat taccagcttt cagacctttc atcattgaca accttggatt 360tgagcaacaa caacattcag ggcaacattc cttatgcact tccacagaaa ctccaggagt 420taaaccttgc tagcaatggc ctcagtggta ctataccgta ttcaatttcg aacatgactg 480gcctcacaga tttgaagttg agtcacaatc aactttcagg tcagatccaa gatatatttg 540gacagctttc cagcctctct actctggatc tatccttcaa tactttgact caaaatctgc 600cccagagttt cagctcattg tcgagtctca gtgtgctata tttgcaaaat aatcagcttg 660ctggttctgt taatgttctt gccaatctgc cactcacaga tttgaatatt gaaaataacc 720gttttagtgg ttgggtaccc aatgcctgga ggtccaacca aaactttaaa tatagcagtg 780gtaatagttt cgccactggt cctgctcctc ctcccccacc atatactcca cctccacctt 840ctaacaatcg gccacccaag tcctcaaatg tggtcccttc atcaggtggc tcaaagggtg 900ggaacagcaa taagaaatct ctgagtggtg gtgccatagt gggtataata tttgcagtta 960ttttgactgt tgttgctgct atattaggag ttattttata tgcacgtaag tctcctagaa 1020gagagcagga tgaagaaaaa ctaagcaatc gtgtgtcttt caccccccta tctcccctcg 1080atgctgaatt attgaaagag agtccagagc aaaaagtcag ctcatcacct cttgaaatcg 1140ctcttaagcc tcccccttct gaacgcaaca agtctacagg ggacaaaggc ttcggaagta 1200ttttttcaag taagaggact aaaaacccaa tatcagcaac tgaatattct attgcagacc 1260tgcaaatggc aacaaatagt tttagtcaag ataatcttat tgcggagggt gctcttggac 1320gaatctaccg agcagagttt ccagatggaa agattttggc agtgaaaaaa ttggacactt 1380ctacgctgtc cctacaaagg cctgaagact tcctggacgc agtatctaat atatcgcgcc 1440tacatcatcc taacattaca gaactagtgg gttattgcac agaacatgag caataccttc 1500ttgtgtatga atatttcgac aatggatcac tctatgacgt attgcacatg gcagatgaga 1560ctactagaaa tttgtcttgg aacattcgtg taaagattgc gctgggttca gctcgagttt 1620tagagtattt gcatgaagtt tgctctccat ctattgtgca taaaaaattc aagtcgtcta 1680atattttgct tgatgatgat ttcaaccctc gtctgtccga ctgtggaatt gcggccctca 1740atccaaattc tgagcgtcag gttcaggtgc tgggttcatt tgggtacagt gctcctgaat 1800atgtcatgtc aggaatctat acaatgaaga gcgatgtgta tagttttgga gtggtaatgc 1860ttgagctttt gacaggccgg aagcccttgg atagttcaag aacaaggtca gaacagtcat 1920tagtaagatg ggccacccct cagcttcatg atattgatgc attggcaaag atggttgatc 1980cagctttgaa gggaagttat cctgcaaaat ctctctcgcg ctttgctgat attattgccc 2040tctgcattca gcctgaacca gaattccgtc ctccaatgtc tgaagtggtg caagcattgg 2100ttcgtatgat gcaacgggct agcctcaata agaggatgac aggagatgaa actgcagacc 2160acgatcctgc agattattaa atgcagtgat attgctttgg tggtactcca gaagaaaata 2220tttaattcta aaaagctcgt accgccttct ttggactttc caaaacagtt gaagagttga 2280taacttcatt atttagctcc taaggccttc gttggacttc cacaacagtt gaaagagttg 2340ttagcatcat ccttcgttgg acttccacaa cagttgaaag agttgatagc atcattattt 2400tcataaattg aatctgttca agattttttt tagtccacgt tgtggtgaaa acaatgtaat 2460tggaatatta ataaaatatt aatactggtt tgctcaaaaa aaaaaaaaaa aaa 2513 14 374 DNA Pinus radiata 14gttaggggtt taaaacctga ggaggctgct gtgtagggtc gagtttgagc ttgctggcgg 60acgggagggg gcgaggctca taacacaatg cgacacaatg cgggttggat gtctgtcaag 120ggttttaaag cctgaggagg ctgctgcgta gggttgagtt cgagcttact cgaggacccg 180agggggcgaa gctgatgatg aggcttggag tgtgaatccg tggatgtttt agcgggacga 240aggatcgagt ctcgtccgtg aggggaaatc aggcagttga aacaaagagg tgaagaaatt 300tgcagcagag tcatgcagca gccgtatgta gtactggccc tgtggtggat attggtactg 360cgtcacccgc tctg 374 15 490 DNA Eucalyptus grandis 15gtctaaagaa gcttgaggga tagacaagcc aaagtaagag agatagagag aaccagttga 60tccaataaag gtcagatttt tatgctttgc tcacacacac ccatctgaag ctactttgtg 120tcatgtcatt cccctatcct tctcctcaaa cccagcaaca tacaattgct ttgtctaaac 180actagtggtc tatgtctgaa agcaacattt tctttgagaa tcaatgaggg tcctattcat 240tgttctgggg gttgtacttc tgtgcactgt aattcctggt tcatcttctt ctgtcagtga 300tgttgatgtc ctacttgcgc tgaagcaagg attccagtcc cctgaaccgg ctttaatcac 360ttggagttct tcaaactcga gctcagtttg cttatggtat gggattagat gctcaagggg 420acgcgtggtt tcgctccagc tgacggattt gaatctgggc ggctctgtct cccctcctgt 480ttcaaggctc 490 16 468 DNA Pinus radiata 16gtttttctca tgctgctgtg ctttgtgctc gttgcaggga cctctgctgc tgtagacgat 60catcaatatc tgtatcatcc ttctcattat gttatgaatg agcgccagga atctggaagc 120tccatggccc gccacgagaa aagggatgtg gaagctcttc tctccttcag aaacgccata 180acggccgacc cacatggatt gttgtccaac tggacggccc acaactctgc caacatatgc 240tcgtggaatg ggatcggctg cagaaaacag agcagaagag ttgtttccat ataccttcgt 300ttctcacacc tagaagggac gctttcgcct tctgtgggta atatctcttt actgcacacc 360ttcgtactta ctgtcaataa attgacaggg agaattccgc cagagtttgg gcaacttaaa 420gccctccaaa cgcttgactt gtaccgtaat cttttatcaa gttctggt 468 17 518 DNA Pinus radiata 17atagagtgag aaatacagag cctaatctga aggaagttgg agtgatagag tgagaaatgg 60atcttcttct tctcctgctt gtgatgatgg gtgtagcaat gcctactcat tctcaacaca 120cgggtggatt cacttctgtt caacgttttc cttttaatgg acggagtatg atggggaaac 180cctccatagc cgggtatcat gaaaaaaggg atgtggaggc acttctcagc ttccggaagg 240gcatcacatt ggatccatat ggatggctct ccaactggac ggccaataac tcacataacg 300tctgcctgtg gaacggaatt tcgtgcagcc caaacacgaa tcgagtggtt gaaatttctc 360tccgttacgg ccggttgaat ggtacgctct ccccgtatat tgggaacctc tctcttttgc 420ggcatttaga tctttcttcg aatgctttga gtgggagaat tccagcaaaa tttgggcagc 480tgaaagcgct acgaatactt gacctctcca ataatgct 518 18 630 DNA Eucalyptus grandis 18tgggtctctc tctctctctt tctctgtgtg tctcggtcct tgtgctcgcg ggctgtttcg 60ctttaccctc ctctacttca ccgccaatga cttgcggaga gatcattctg ggtctgtgcc 120tgtgacgtcc cttcttggga ttttcgtgct tcattcttct ggtacactcc tcgtttgtaa 180aatcccggtt ggcgagctgg gtggagtgtt tctttgcgtt ttgtggtctt ttaggctctt 240gggtcgtcgt tgttttggcc attgaggccg tttcttgtcc gtttcctggg agagtgaggt 300gcgcagacga gaacggaagg aaaaaggtgg gaactttgaa gcgtggactg tacggagcgt 360cgtgggctct gcgaaattga agatggtttt ccgacgattc gtggtgatgc tcttcatttg 420cacggcgtcg gtctgcgctg gcctcactga tcctcgcgat gtggcggcaa tcaatagctt 480gtatgtttca ctgggctatc cgcctctacg tgggtggtta cttgttggag gcgatccctg 540tgttgacaat tgggaaggtg tcgagtgtgt tatctcaaac ataacgggac taaatctgag 600cggagccaat ttgggtggag aattgggcga 630 19 385 DNA Eucalyptus grandis 19ggcagaaatc ctaagctagg cagagtctcc gacgatacca aacgatttat agttcctgct 60tctcttgttt cgtgatgggt ttcttctacc aagtctttct catatttctg gcggtcgcgc 120cgtctgctct gtgccaagtt actgagttcg tcagcataga ctgcggagga tccagcaact 180acacagaccc gacaacaggg ctcgcatgga tcccggacac gggtctcatg agctatggcc 240agtcttcgaa ggtgcagaat cccaacgtaa gctcggtgca gtattcgacg cgcagggact 300tcccaataga cggccagaag tactgttaca ccctccggac cgaagagaga aggagataca 360tcgtccgtac gacgttcttg tacgg 385 20 496 DNA Pinus radiata 20ctctcctcgt ctctgcattt caagcaccat ggcgtttcca tgtttttatt ttcctttccg 60tatcttgttt ttcctcttcg tatgctcttt ttccttctcc ttctccttct ctgcgcacaa 120atttcatgac ggtcggaaat ggctcttgtc attcaagatc gatattacca atgatcctca 180tgcttccatg gccaactgga gtcctgcagt ccacctgtgc aactggactg ccgttacgtg 240cagtcgcagg cacgcagata gggtggtctc tctcgaccta agtggcatgg atctgagcgg 300ctccatatct ccttccctcg gcaatttgtc atttcttcat acccttaatc tcagtgccaa 360tgctctccat ggtcatattc caccacaact gggacgcctc tttcgtttga gaaacctctg 420gttacgcaac aattttctgc aaggaaatat ccctacagag ttcgcctccc tcaaacattt 480gcaacagctt tacttg 496 21 352 DNA Eucalyptus grandis 21gagaactgaa ctgacccaga gacccacttt ttctctcatc atcatccctt caggatcatg 60agattttcgt ctctcaaaag aagaaggtgg atcatgagga cagcaatcat cattaccagt 120ctgatgtgtt gttcttccgc tagagacagc atgacgctca gcagcccgct cagcgatgag 180cacggagaca ctctggtttc tgatggaggg accttccaac ttggcttctt cagccccaac 240ggaagctcgg gctctgacca cagaaggtac cttggaatat ggtactacga ctccgacccg 300cagaccgtag tttgggtagc taacagagac caccctgtgt tggatgtgac tg 352 22 635 DNA Eucalyptus grandis 22ccggtctgaa aaccgacact tttgagtgag aaccggttcc ttttgcggag ttttggaggg 60acgggaagtg taatggggct cgcacgtttg ctcctggttg gcttcgtcct gctcgtgctt 120ggctcgagag cgctcgtgtg cgggaatgcg gagctggagg cgctcatgga gatcaaggcc 180tccttggacc cggagaacaa ggtcctcact tcgtggacca gcaatggcga cccctgcggc 240ggctcgtttg acggggtggc gtgcaacgag catcagaaag tggcgaacat ctcgctgcaa 300gggaagggcc tgtcggggaa ggtccctccc gcagtggctc agctgaagtg cttatcgggc 360ttgtacctgc actacaatta cttgaccgga gagattccaa gagaaatctc tagtctgacc 420gagctgacag atctctacct cgacgtgaat aatttaactg ggagtatacc ttctgaaatt 480ggaagcatgg ctagccttca aggtgagttt tgtttgttgc tccaagggca ggggtgaatg 540ctttcttgtg tatgatttca ctcccaaggg taccttatcg caatatcttg atttagaaga 600tggaagcatc caggttcttg attggtccac aaggg 635 23 580 DNA Eucalyptus grandis 23cccaggagac atcacccatt ttcctaagta ttaccagatc aagagaataa cctgaagctg 60tgttctttgc tactccgcat ccgttatttt cttcggactc tgctccgatg ggagtgaaga 120gagcgaattc ctctctgtgc tgcgtggttt tgctcggctt gtgggcttgc gtacatgggt 180tgctctctcc tagtggtgtc aatattgaag ttctagcttt gatggacata aggaatttgc 240tagtggatcc tcatcaagtt ctgaacaatt gggacgcaaa cgaggttact ccttgtacct 300ggactgcgat cacgtgtgag gctgatgtcg tcactaatct agaaattcca agacagaact 360tatctggaac gctttcgcat agcattggga acttgattaa tcttaaatat ttgttccttc 420aggacaacaa catttcagga tttattcctc ccgagcttgg gaagctccag aaacttgaaa 480tgctcgacat ttccagtaac tcattctctg gtgaaattcc taccgagcta tcccatctca 540agaacctcca acaactgaga atgaactaca ataacctttc 580 24 457 DNA Eucalyptus grandis 24ctcacctccc aaagaccaaa catccccaaa aatcatggtt tcttccttat tgctgtccag 60ccagcctaat ttagtggtcg gcttcctctt cttgctcctc ttagttgaca ccactccatt 120tcacggagca ggcgatatgg cttcctccac ttcctttttc accatcaaca tgaccaagag 180taaggatgaa gtagaggctc tcttgaattg gaaatctact cttgacaact atagccagtg 240tctcctctct tcatggcatg acaacaatcc ttgcggcttt agcggtgtca cttgtgacga 300ctccaaagct gtcaacaatc taaatctttc caatcttggt ttgagaggaa ctctagacgg 360tcttgacttc tcgtgcttaa ccaatttggt tacctttgat ctttcctaca acgcaatcta 420tggctccatc ccctcaagta ttggtaacct ttccaaa 457 25 481 DNA Eucalyptus grandis 25ggaagagtgt aacttcttct tcttctccat ttgaattgtt tggcgctgtt tacagatgaa 60gattggagtg acgagacaca tctttttgct gaggaaggag tgtgaaggtg cttcaatctt 120ccccagaagc cggggaggct agaagagctg ctcgttcttc ttcgagccta actattctcc 180aagtatattg ctgtgaactg gctgtagaaa atggaaggga agcacctcgt ttttctgaca 240attctcttgt tggaatcgat ttgctcgaat gtctcggcga tacccaacga ggataagcaa 300gcattactgg attttcttgg caatgtttcc ctctcgcgcc ctctcaattg gaacaaggat 360tcttctgtgt gccggtcctg gacaggggtc aagtgtaata acgaccaatc gagggttgtc 420gccctccagt tgcccggagt ggggatcaaa ggtcgaatac cgccgaacac actgagtcgc 480t 481 26 648 DNA Pinus radiata 26tgattccatt gcgctgaaac caagaactct gtctgtctga tcaggctgta tcatggccta 60caagcttttg agattttggc ttgaatttca ggccttacta gtcctctttc cattggctct 120gagtgtctct caggagggct tggttttgct ggaagtgaag aaagaactga gtgaccccaa 180tggatttctt ggtaactgga aggcagagga cgattctccc tgcaaatgga ggggtatctc 240atgcgaccag aggtcaaaat cggtggtggg gattgaccta agcagcggag gtttagttgg 300ggtttttcct agtgtcgttt gcaatcttcc acagctgaaa aatctgtcgt tgggggataa 360caatattggc tcaatattac ctcgcaacct ttccatgtgc aggcagcttc agcgcttgaa 420tttatcgcag aatctttttg tggggaacct tccggacttt atttcagagc tcgcagagct 480ggaatacttg gatctctcga gtaacaattt ctctggttcg attcctgcag gcattgggaa 540actgccaagg cttcaagtac tgaacctgtg ctgcaatctg ctaaacgaga caatcccaac 600atttctggga aatctcagca atctgcagca attattgctg gcatacaa 648 27 427 DNA Pinus radiata 27atacattagt tctgcaaatc aagaatggga tcgtcggcct ccccttttat ggtgttgtta 60ctcctttcca ctttgattct tgaaatattg ccaatgtatg ttgcaacaaa tagaagcgaa 120gctgatctcc aaggtctcat agcattcaag gcatcaatca ccagtgaccc cttgaatgct 180ttagctgatt ggactgcttc tgctcatcac tgtaattggt ccggtgtggc ctgcgatccc 240ctccacaacg ttatctccat tagcttacca gagacacaaa tccaaggcct catctctccg 300tttcttgcaa atatatctta tttggcttca ctagacctca ggtccaactt cttccatggt 360gtcattcctc cgcagctcgc tctgtgctcc caacttatcg atctggagct ctttaacaat 420tctttga 427 28 516 DNA Pinus radiata 28gctgaacata actgttttgc ccattctgat tttgctgaag caccaaggct tcataagagc 60aaaatggaag tacggatgat aaccataatg atattatgtg ctgctttact atgtgaaggg 120agcggagagg tggatgttct gatggagatg aaagctgcat tggaccccaa gggggagata 180ctctactctt gggtgaaggg aggagatccc tgcagtggga cttttgatgg agtggcctgc 240aatgagcaag gaaaagtggc taatgtttct ctgcaaggga aaggattatc aggctcaata 300ccctcaacca ttggcaagct caagtgtctc actggacttt atttgcatta caacagcctt 360ggaggggaga tacccagaga gctttctaac ttaactgagc tgctggacct ttatcttaat 420gtcaatgggc tctctggtcc cattcctaag gagctgggag ccatgtccag cttgcaagca 480ctccagctgt gctgcaacaa gttgacaggg cctata 516 29 418 DNA Pinus radiata 29ggaagttgga gtggtagagt gagaaactga cagcctaatc tgaaggaagt tggagtgata 60gagtgagaaa tggatcttct tcttctcctg cttgtgatga tgggtgtagc aatgcctact 120cattctcaac agacgggtgg attcacttct gttcaacgtt ttccttttaa tggacggagt 180atgatgggga aaccctctct gttcccatcc tccatagccg ggtatcatga aaaaagggat 240gtggaggcac ttctgagctt ccggaagggc atcatatcag atccagttgg atcgctctcc 300gactggacgg ccaataactc acataacgtc tgcctgtgga acggaatttc gtgcaggcca 360aacacgaaac gagtggtttc aatttctctc cctgaatgct tgttgaatgg tacgctct 418 30 468 DNA Pinus radiata 30catgagggag agagagacca aggtctgtat cttcattttc tgtattttca catcatgggc 60tctggtcgat tttggggttc ctgcagtttt tgcagcacaa gagactcaga tacaaatact 120cctgaggatg aaagaggctc tggaggatcc cacaaatgca ctcagggatt gggatgggtc 180tgaggattct ccatgtagat ggagagggat tgattgtaac gatgaaggtg ctgtgactcg 240catacaactc catggaagtt ctctgagtgg tcgcattctg cctgatatct gcaatctcca 300gagcctgata atctttgagc tagatcgaaa ttccctttat gggaatttcc ctccagaatt 360ttcgaattgc agtcggttgg aacaattgaa tttgagctcc aatttgctga acgggtcatt 420gcccgacctt tcaaaattga aggctctgaa atatctggac ctgtctaa 468 31 409 DNA Pinus radiata 31ggcagttgga gtggtagagt gagaaataca cagcctaatc tgaaggaagt tcgagtaata 60gactgagaaa tggatcttct tcttctcctc atcatgatgc ttgtgatgat gggtgtagca 120atgcctactc attctcaacg ttttcccttt aatggatgga gtatgatggg gaaaccctct 180ctgttcccat cctccatagc cgggtatcat gaaaaaaggg atgtggaggc acttctgacc 240ttccggaagg gcatcacatt ggatccatat ggatggctct ccaactggac ggccaataac 300tcacataacg tctgcctgtg gaacggaatt tcgtgcagcc caaacacgaa tcgagtggtt 360tcaatttctc tccgttacgg ccggttgaat ggtacgctct ccccgtata 409 32 518 DNA Pinus radiata 32gtagagtgag aaatacacag cctaatctga aggaagttcg agtaatagag tgagaaatgg 60atcttcttct cctcatgatg atgcttgtga tgatgggtgt agcaatgcct actcattctc 120aacgttttcc ctttaatgga cggagtatga tggggaaacc ctctctcttc ccatcctcca 180tagccgggta tcatgaaaaa agggatgtgg aggcacttct gaccttccgg aagggcatca 240cattcgatcc acatgaatgg ctctccaact ggacggccaa taactcacat aacgtctgcc 300tgtggaacgg aatttcgtgc aggccaaaca cgaaacgagt ggtttcaatt tctctccctc 360agcgctcgtt gaatggtacg ctctccccgt atattgggaa cctctctctt ttgcagcaat 420tagatctttc ctttaatgct ttgagtggga gaattccagc agagtttggg cagctgaaag 480cgctacgaac atttgaagtc cgccataatg ctttgagt 518 33 401 DNA Eucalyptus grandis 33gttcttagat cctgaagaac ctatggcgtc ttgcggtggc cttgtgtcta ccttcttgtt 60ggcaatcttt gtcactcaaa ttgtcgaatt cagtcattcc attgcttcga caaatgtaag 120ttgtattggt gtggagaggg aagctcttct aaagttcaag catggtctca ctgatccttg 180gaaacgcttg tcatcatgga ctggtgagga atgttgcaag tgggaaggag ttgaatgtaa 240cgagaagaca ggccatgtcc tcaagctcga tcttcataat ccatgtattg aagagattga 300tatgcttgaa ccttcatata agtgcaggtt gggaggtaac atagttcatt ctttaacaga 360actgaagtat ctgaagcacc tggatcttag catcaacaac t 401 34 434 DNA Eucalyptus grandis 34ggagaaccca acattgcttt ctctgccact ctcactctcc ttctttcaat gtgttccaga 60agagttgttt cttcaatgga gaccaaccca attcatctaa tctaccatgt atttttcatc 120gtcatagggc ttgtctctgt ttctgcagcc gaacagagtg cttcctccag gaaaacggat 180gcggaagccc tgatcctgtt caagaaaatg atccagaagg acccgagtgg agtgctatct 240gggtggcagc tcgatcagga tctctgcgct tggtatggag tcacatgtta ctcagggagg 300gtcactcaac tcgatcttca cggccaaagt cttgaagcga ccatgtcttt cgaccctttg 360agttctttag acatgttaac tgtctcaatc tgtcatcaaa ctcgttcgcc atcgattcaa 420cttccttgct tcaa 434 35 442 DNA Pinus radiata 35ggaagttgga gtggtagagt gagaaatact gaaggaagtt ggagtgatag agtgagaaat 60ggatcttctt cttctcctgc ttgtgatgat gggtgtagca atgcctactc attctcaacg 120ttttcctttt aatggacgga gtatgatggg gaaaccctct ctcttcccat cctccatagc 180cgggtatcat gaaaaaaggg atgtggaggc acttctcagc ttccggaagg gcatcatatc 240agatccacat ggatcgctct ccgactggac ggccaataac tcacataacg tctgcctgtg 300gaacggaatt tcgtgcaggc caaacacgaa tcgagtggtt tcaatttctc tcccttactg 360ccggttgagt ggtacgctct ccccgtatat tgggaacctc tctcttttgc ggtatttata 420tctttcaaat aatgatttga gt 442 36 493 DNA Pinus radiata 36gctgctcggc actctcagac gaagttacga accatgaggt ccgtgtttcg aatctcgtcc 60cttctcgtga tttctattct ggttctgctc acgctttctt ccgccattaa cgacgatgcc 120gagatgctgc tcgcgttcaa gtcagccatg tcggatcccg acggcgccct ggccgggtgg 180actgagtccg acgccgccaa tttctgtggg tggactggag tcctgtgcaa cgaattcaac 240agaacgagtt cgctggactt gaccaacatg aatctgtcgg gcatcattcc gccccggaca 300ttatcgagcc tcgacagtct cgtgaatctc agcttggcgc tcaacaaatt cagcacgccg 360ttcccgtcgg cgatcctcga catttccact ctgcgattcc tcaatatatc caacaataac 420ttcagcggcg agatcccggc aaacatttct cggttagtga atctggagtt gctggacacc 480tacaacaaca att 493 37 536 DNA Pinus radiata 37ggagaagctg ctgctcatgg ctcccctgct gggaattctc cttcttcatg ctctcatttt 60tagctatgga gaagaagctc acgtcgctgg ttcgaaccac acagaggttc gggcactcat 120ggctctcaag gccgggattg ttgacacttc aggtcatctg agtgactggg aagtccatgg 180cgatgaactt agtgcttcgc cttgttcttg gactggtgtg ttttgtgatt tggagtctga 240gaacgtgacg gaactcgatc tctcacggat gaatctcact gggacgattt cagacgagat 300tcgggagctg cagcacctga aagtactgaa tattagcttc aatcagtttt ccggggcctt 360tccagttgta atctttaatc tcaccaggct gcggagcctg gacataaatc acaactcttt 420cgaagggtat ttcccggccg ggatctcgaa gatgaagaat ctagtgaatt ttatagcttt 480tagcaatagt ttcaaagggc ctctgccgct ggaattcgtg gagatgcttt tctgga 536 38 822 DNA Eucalyptus grandis 38ctctctcttc accaccactc ctcctcccct caccttaatc tctcctccga tcccacacca 60aaacccccgc cccacctcca tggcggcccc ctcctcctcc gccgcctccg ccgccgtgtt 120cgccctccac tcgctgctgc tgctgctgct gctcgccgcc gccgtcgagg acgacgtgct 180ctgcctcgag ggcgtcaagc gctccctcgg cgacccccag ggttccctcg ccgactggac 240cttcgccaac acctccgcct cccacatctg caacctcaac ggcgtcgcct gctggaacct 300caacgagaac cgcatcatca gcctcagcct caccggcttc ggggtctccg gcggcctccc 360cgagtccctc aagaactgcc acagcctcca gacgctggat ctctcccaga acaagctcga 420cggccccatc cccgcccaaa tctgcgagtg gctgccttac ctcgtcaagc tcgatctctc 480ctctaactcg ctcgccggcc ccatccccag ccagatcggc gactgtaagt tcctcaacaa 540cttgattttg aacgacaaca agttgaccgg ccccattcct tacgaggtcg gtcgcctgga 600ccggttgaag gtgttctccg tccggggcaa tgatctctcc ggatctatac cgtcggagct 660gtccaaattc agttccgacg atttctccga caacgacgat ctgtgtggta ggcctcttgg 720gtcgtgtggg gggttgagta agaaaagcct cgctataatt atcgctgcgg gtgttcttgg 780agccgcagca tctttgttgc tagggtttgc gctctggtgg tg 822 39 454 DNA Pinus radiata 39ggagtggtag agtcagaaat ggatcttctt ctgctcctcg tcatgatgtt tgtgtgctct 60gtgatgatgg gtgtagcaat gcctacggat ggattcactt ctgttgaaag cgttcctttt 120aatggacgga atcgccataa aagggatgtg gaggcgcttc tgagcttcaa agagagtatt 180atatccgatc catatggatc gctcaccaac tggacggcca ataactcaca taacgtctgc 240ctgtggaacg gaatttcgtg caggccaaac acgaaacgag tggtttcaat ttctctccct 300gagtgctggt tgaatggtac gctctccccg tatattggga acctctctct tttgcggcat 360ttagatcttt cttggaatgc tttgagtggg agaattccag cagagtttgg gcagctgaaa 420gcgctacgaa tacttgacct ctctgctagt catg 454 40 504 DNA Pinus radiata 40caaaccctct gaaatatttt tgtaattcct tcattttatc gatcgcccat ctgaggagcc 60gatggcacct tccacagatt tcatcctcat cacctccaca ctcatgctca tctttgtttc 120tgcaaacgcc catcttcttc atcattatca tgagaaaagt cgtgaacgac tgcaggtcga 180tatagaagcc ctgcaagcat tcaaggcctc cctcacatat gatccttctc acgccttggc 240caactgggat tttgtcgcca atcatgtctg caattggact ggtgtcacct gcaaccctca 300caaactgcgc gtatctgccc taaatctcta caacatgagc ctgcaaggga ctatacctcc 360acatttgggc aatatctcct ttcttggcgt gttgaacctc actttgaaca gtttctctgg 420cataatccca aacgaattgg gcaagctgcg tcggttaaaa cgtctgtccc tcaagcagaa 480tcagttgatt tcgtccattc caga 504 41 403 DNA Pinus radiata 41cattccttct cttctcgttt gtgcaatgag aatgtttcta ttactcgttt atttgttccc 60ccttgtcact cccttcgcgt tttccacagt gcaattatct aatggctcta atgctgtcga 120tcaagaggct gtgcttggat tcctgtctgc aatcactaat gatccctacc aatcgttacc 180cactaactgg aaatcaaatg tttcagtctg cgagtggaca attatcaaat gcaatgggtc 240cagagtggtg tccctaaatg tgtcgagcat gggattagaa ggtacaatct ctcctcttct 300cggcaatctc tcatttctgg aaaaactcga ccttcgcaat aacaattttc atggtcccat 360tccctatcaa ttgggaagcc tggtgcgctt gcagatgctt atc 403 42 595 DNA Eucalyptus grandis 42gagagacggc gatggaggat gaggaagaag agacgacgac ggcgacgatg atgcgcggac 60catgccgatc cagagacggc ggcgcagcag cggcggcggc ggcggcggag gcggcggggc 120cttccaccgt gtgttgattt ctctctcccg gccctcccga ctccgccttc gggctcggcg 180gacttgatta gtcgggcatg tgaaaaagaa tctttgactg ctcagattgt tgaacaacgt 240gatggagtcc tgcaactgcg ttgagccaca gtggccagct gatgagcttt tgatgaagta 300tcagtacctc tcagatttct ttattgctct ggcgtacttt tccatccctc tagaactcat 360ctactttgtc aagaaatctg ctgtatttcc ctatagatgg gttcttgttc agtttggtgc 420cttcatagtt ctgtgcggag caacccacct gatcaactta tggacatttg ccattcactc 480aagaactgta gcatatgtta tgaccattgc aaaggtttta actgctgcgg tatcatgtat 540tacagctctc atgcttgtgc atatcatccc cgatctactt agtgtgaaaa ccagg 595 43 397 DNA Pinus radiata 43gttcgttagc aatgcccact tctacctcaa agtgcaggtt aaggactctg gctgtggcat 60acagccacaa gatattcctc aaatatttac cagatttatt catcctcgaa gtggatctaa 120tcgaggtaat ggcagcggag gacttggact tgccatttgc aaaagattta taaatctcat 180gggaggtcac atctccatcg agagtgaagg ccacgacaag ggcaccatcg tcacttttgt 240cgtcaaacta cagaaatgca gcaatgcaaa tgactcggca gcccacgaga tcacatctag 300agctcagtct attcatgaaa gcacccattt tgctcggcat aaacctctca tagacactga 360cagaacggtc ccctccagct cccagtatca aagaagt 397 44 415 DNA Pinus radiata 44ataaattctc tcaaagagat ggtatgcact tatggtcctt atgctgccct ctgatagtgc 60tcgcagatgg catgttcatg agttggagct tgtggaggtt gttgcagatc aggtagcggt 120ggctctctca catgcggcaa ttttggaaga atcaatgaga gcacgagacc tgctcatgga 180gcaaaatgtt gcacttgaga tagctcgaca ggaggcagaa acagctattc gcgctcgcaa 240tgatttctta gcagttatga accatgagat gcgtactccg atgcatgcaa ttattgcttt 300gtcatcgctt cttcaggaga cagagttgac tcctgaacaa cgatccatgg ttgagaccat 360cttaaggagt agtaatctcc ttgcaacact catcaatgat gttttagatc tttca 415 45 526 DNA Pinus radiata 45atggcgagtc caccctcgac tccaacaggt atcaccaacg tagctcgtct cacgatgcgt 60gctgaagccg gtggtgatgg tgacagtcac gcccttgccc tcggtagcga tccgatcgcg 120acccaatcgc catcgacgaa gcgtcgtgcc gcatccgcgc catggccatg ggcgtatacg 180cgtgtaccca ttgtggcggc gaccgcgggt gcactggaag aagagcagaa agagtgtatc 240caagcaggta tggatgatgt cttgacgaaa cccatcgaca ggtatcagct gcaaaggaag 300ctggccaggt tctcaccgcg tttcacatcg ctcgtagtag catcatctgc accagcaagc 360cagcaagccc atcagtgaca tgtggcaata tgacatgcac aacagcatat taaagtcctc 420tagttggtca tcataaccag caatacaagg caacaacaag caaatgcatt tcaatgtcat 480agtcactgtt acacatcgcc accttgtccc aacttatcac catacc 526 46 255 DNA Pinus radiata 46gacaagagtg ttgctgattg atgaccatcc actgttccgg gagggactgg caggtgcgat 60ccaggccgag ccagatttcg aagtcgtcgg ccaggccggg accgtggacg agctgcgcgg 120gcttgcgccg cagatcgagc cggacgtcgc gatcgtcgac ctgttgatgc cgtcggtctc 180cgggatcggc gtcacccgcg agctgtgcga gctgctggct aggtgccgag tgctggggct 240gtcggtcttg gtcga 255 47 3567 DNA Pinus radiata 47aggcaatgca agggcaggca tggaggactt tttaaggatc gtgtagttgt taatgatggt 60tcaagtgggt gcatctcaaa attttaaaca attatattga aggacttttg ggactttgta 120taatatttga ctactatggg aaggatgggg agaatgcatg aatgtaaact gtagtccatt 180cccctcccaa gtatcgagaa ttctttggat ttggattaca tttacatgtc attctcatat 240tctgtgggtt ggcaagacca ggcggtatgg aagttgctgt gggtggctgt actgttcatt 300ttcattaaaa gaatacagag gccaaagtct gtattaatcc cacgaagtca agctcggcat 360tcatattttt tcctcctggc atttggttgc ggtactaatc cacaaatggc aattctgaat 420atatgaattc tttctcttgt ggaaacctgg aaggacagaa accctcgatg aggttaagtt 480tggtctgtat tcgttgagag gttcttcatc tgatatggat tggaccagaa ctggggcaca 540tcatgtttat gttcaaggag aggcaatggg tttggtcgat aatccccagt ctcctagatt 600tgggtttttg tttcgtgtga atgtaaatac aaattaagtt aagaaattcc tttggaaaac 660ccatattagg gctttgggcc tacatgggta ccattggaca ccgatcagaa gggcggtttg 720agtgcgctgt ataagtggtt tctgttttga ttcagaggag cccatgtgtg tgatccgagc 780agattcaaac gcgtgacttt gtttgtgttt tttcaaaagg ccgctgctcc ctgttatcac 840acagttcaca tgccgtaagg acttgacgag attgttgcaa tgatctgtct gggtgggatt 900gtcattgttt cattcctcct ccagaacatt gcaatggcat cagaggatgg gggttccaga 960tgcaactgtg acggggaagg atggtggaat gttgagaaca taatgcaatg ccagatggtc 1020agtgattttc ttattgcctt agcgtacttt tccattcctc ttgagcttct gtactttttg 1080agctgctcca atagtcttcc attcagatgg gtcatagttc agtttggagc attcattgtg 1140ctttgtgggc taactcactt tatcaacatt tggacatatg gtcctcagtc tttccatgta 1200atgctggcac taaccatctt caagttctta accgcccttg tttcttgtgc cacggccata 1260actcttgtaa ctctaattcc tgaattgcta cgggtgaagg taagggaaat ttttcttaaa 1320aacaaggcaa gggaacttga tagggaggta gacattgtga aaaggaagga ggaaactagt 1380tggcatgtcc acatgctaac tcaagaaata cgaagttctc tagataggca cactattcta 1440aatactactt tgatttcact agcaaagacg ttgaatttgg aaaactgcac tatatggatg 1500ccactggcag atggtactgc catggaggta tcccatgagc tcaaacgacg gcatttgcaa 1560gtaccattaa ctgttcccac aaccgaccca gatgtgaaaa agattatgca tagcgaggat 1620gcaatccttt taagtcctga ttctgccttg ggaaaggaga gtaatcacca ctggtcatta 1680gccgggcctg tggcagcaat aagggtgcct ttgtggaagg catcaaattt caagagtgga 1740gcatcggtag atagagaaga atcatatgca ataatggtgc tagtcttacc ttgtgaagat 1800gaaaggcagt ggagttctca agaattatat attgtcaaag atgtggcaga acaagttgct 1860gttgccctat ctcatgcagc tgttttagaa gaatcacaga agctgaaggc tccacttatt 1920gataagaata aaacattaca acaggcaaaa caggatgcat tgagagcaag caaggcacga 1980cattcttttc aattggcaat gaaccgtgaa atgaggcttc ccatgcatgc tatttctgca 2040ctctcatcta ttttacaaag tgctagactg aatgttgaac aactggctat gacaaatatg 2100ctagctaaaa gtagcagcct tctctcaact ctcataaatg acataatgga tttttcagaa 2160ttggaggata cttctttggt tcttcagtta caccccttcc agctacatgg tatgctgaaa 2220gatgcagcac acctcacaga gaccatgtct agaagcaagg gtcttctatt aaatgttgaa 2280ataggtgatg ggatgcctga ccatgtgata ggagatgaaa aacgaattct acggattatt 2340ttacatatgg ttggaaatgc aatcaattca accaaacaag gaacaatatc aattcgcatc 2400tgtgtcgaag acagagctga aggttggtgg gaccccaaca atcgacgatg gagaccctca 2460ctatgcgaag gttttacata tttaagattt gagatcagga catctggctc tggaagtatt 2520caaaatgaca atcccagttt cctgactgtt gtacaagatg gtaaaagtga ttcgtcatca 2580tcaactgggg aaggacttgg ttttgctatt tgtaaaaagt ttgtgcagct tatgcatgga 2640aatatttggc ttgagcctaa ctccaagggt gaaggctctg ttgtgacctt tttaattaga 2700gtacaactgc agacatcaac tgcaaacaaa cattggctgt ctccagatga gaagatttac 2760aagtcttcat ttaaaggttt gaaagttctt gtagcagatg acaataatgt aagtcgttca 2820gttacgagaa ggctgcttca agagctaggt tgtcaaacaa ctgaagtaga ttcaggttac 2880aggtgcttaa tgactttgct tcagtctgga agtgcatttc aattggtctt tctggaagtt 2940tgcttggccc agatggatgg atatgaggta gccttccgta tacgacagaa attcagatca 3000agaaaccgtc ctttggttgt ggctttaaca gctagtacag acaaggagac catggagagg 3060tgccttcaaa ctgggatgga tggtgttata cggaagcctg ttacgttaag agaaatgagc 3120aatgagcttt ttaaaattgt acatcagaca aataacatcc acgaatagtg actacaagtg 3180tactttgttc tgaggtgcag tccatctggt gaagattgat tgctttgaca ggttcagagg 3240agtgcaaatt atggtgacaa tgagaccatg ctacacagtc agttacgttg gagattcctg 3300tgcttaaatt caaaaggtcc atggtcttct ccgagtgtta agaaacacgg tactttgcca 3360tgtacttttt tccattgcta ctaaaatcat tctgtcacac acaggttctg tatgggtaat 3420tacctcatct catgcatggc gatgttagaa gtcctgggac ccatttgaca agtcactgac 3480tgttttagct caattcttac agaagatgaa atcaaaatta tattgatatg aagctcatga 3540ttctttattg tgatcaaaaa aaaaaaa 3567 48 476 DNA Eucalyptus grandis 48agacgatgcg agcgaaacaa atgctagcaa caatgtcaca tgagataagg tctccccttg 60ctggggttgt tagcatggcc gagattcttg cacaaactag acttgatcat gagcagcggc 120aattgttaga tgtcatgctt tcttcgggag atttggtcct tcaactaata aatgacattc 180ttgacctttc gaaggttgag tcaggggtaa tgaagctgga agctacaaaa ttccggccga 240gggaggtagt aaagcacgtg ctgcagactg ctgcggcatc attacggaag atattaacat 300tggaaggaca tgtagcagat gatgttccta ttgaggtcat cggagatgtt ctaagaatta 360gacaaattct caccaacttg atcagcaatg ccatcaaatt tacacatgaa gggaaggttg 420gcataaatct atatgtggtt ccagaaccat ctgtcgagaa aacagaagaa tgtcct 476 49 513 DNA Eucalyptus grandis 49gtaagattga gattgaggct gtgcagtttg atctaagggc catattggat gatgtcttgt 60ccctcttctc ggggaaatct caagaaaaac gagtggagct agcagtttac atttctgaaa 120atgttccgga gaagttgatt ggtgatccag ggagatttcg gcagatcatc acaaatctta 180tggggaactc aattaaattc acagagaaag gacatatctt ggtcacagtc catcttgttg 240atgaggtgat gaactcaact gatgctgaga tggaatcagc aacaaggagt acgttgagcg 300gcttcccagt accagacaga cgactcagct gggcaaaatt taggacattc agccaggaag 360gccctgcgtc tccggtgccg tcatccttct ctaatccaat caatctcatt atttcagtgg 420aggatacagg gataggtatt cctccagagg cgcaaccccg cgttttcact cgtttcatgc 480aagtgggccc atcaatttct cgaacacatg ggg 513 50 1995 DNA Eucalyptus grandis 50cttgagcata aaatatccat ttcttcactt aagacaaagt tgaagcaaca tggtagtcac 60gcaagaaggg catccaagaa agacaacaaa gtgaccctct ggttcgaagt tgatgacact 120ggctgtggaa ttgatccaag caaatgggaa tccgtttttg aaagctttga gcaggccgat 180ccatcaacaa ctcgattaca tggcgggact ggtcttggcc tatgcatcgt acgaaccttg 240gtcaacaaaa tgggtggaga aatcaaagtc atcaagaaga atggcccggg aactctcatg 300agactttact tactcctcaa tgcacctgtt gatggcacgg aacataactg ctcagtggat 360tatgccgtgc acaacataag agtgctactc gcacaacatg gaagcacggg tagatttata 420atgtctggat ggctgcgcag aaatggagtt tccactctgg aagcatctgg atggaatgaa 480ttgacccaga ttcttcagga actctaccaa ggcagaaatt caggtgctcc atacaggact 540gttaatacgg aacatgcaca tgaactcccg agatcagaag tgacaacttt cgatgatatt 600caaagtgaga tcctcatcat agtcgtggat atagagctac ttgacctaaa cacagatata 660tggaaggaac agctcaattt cctggacaag taccacagga aggcaaagtt cgcgtggatg 720ctaaaccatg acaccttcaa tgccattaag gtggagctcc gaaggaaagg acacatgctg 780atggtcaaca agccgctata caaggccaag atgattcaaa ttctggatgc tgccataaag 840gagaggaact ctgaactcct gaaaagggcc tccaattctt caaaaagcat gaataaagaa 900gaggacttgc acgagtgtct agaaatcgac tctgagcact atgagggagc gagctctgat 960gaactggaca cagttgaaac atcacgttct ggttgtacca atacatctcc tggtgaacaa 1020aagcaacagg aagggatcaa aacccctcct gctctacaac acaggacatc gaactatcac 1080tcattcaatt ctactctgct gtcttccgac tataacaatt tagggaacaa agaagaagcg 1140tgtccaacta gtcccccttt ggaccaccca gataatgccg aaggcagatt caagtgcacg 1200aggagcgtgt tttcttcaaa agaaaaggaa gatggaaatt cagaagcaca ggaacaactt 1260ctgatcagca agcgtcctcc agccaaagtt gattcgtgtt ccagtaaaga attggaccag 1320aaagggtctc tggagggcct gtgcatacta cttgccgaag acacgccagt gcttcagaga 1380gttgctacga tcatgctgga aaagttgggg gctaaagtca ttgctgttgg agatggcttg 1440caggcggtga atgccctgaa cagcagtcta gatgtagatg cagaagactt caggacgacg 1500ttgcatttgc aaaacgcgaa caggatgcct caagcaggaa cacgaagttg gcaaccttac 1560gacctgatcc ttatggactg tcaaatgccc cagatggacg ggtacgaagc aacgaaagca 1620atcagaagat ccgaggccgg aagcggcctg cacattccga ttgttgcgct gaccgcgcat 1680gccatgtcat cagatgaagc caagtgcttg gaggtgggaa tggacgcata cttgacgaag 1740ccaatcgact ataagcttat ggtgtccaca attctgtcgc tcaccaaggg agtcaactga 1800gagaacctgc taagacaact tagagagaac caaagaaagc cagattttaa agtttacaca 1860gtttttgctt ttgaaatgtg aaagaatcag tatgattaac gaactgggca agtataccag 1920tgaccccatc tgcaatctgt aaagacatct gagtttctct agcttatttc tgagcatatt 1980cggaaaaaaa aaaaa 1995 51 461 DNA Eucalyptus grandis 51ctctttctac cggcgcttct tcagcaacaa ctggcatggc gagcaacggc ttggtctcgc 60cgcggaggag gtcgtcgggc cagtttgacg gcagcgaccc gtctccgtgc ggttccgagg 120aggtccacgt cctcgccgtc gacgacagcc tcgtcgatcg gaaagtcatc gagcgcttgc 180ttaagatcac ttcctgcaaa gtgacggcag tggatagtgg actcagagcc cttcgatatc 240ttggtctgga tgaggagaaa acggctggag attttaacgg gttgaaggtg gatatgataa 300ttaccgacta ttgtatgccc ggaatgactg gttacgagct cctcaagaaa atcaaggaat 360cctctgctct gagggaaatt ccggtggtga tcatgtcgtc cgagaacgtc ttggcgcgaa 420tcgacaggtg tatggaggaa ggtgcggagg atttcatcgt g 461 52 570 DNA Eucalyptus grandis 52atcatttctt cctgcgcttc ttcagtaaaa actggtatgg cgaggaacgg cgtggcctcg 60tggcggagga ggtcgtcgga ccagttcgac gacccgtcgc cgtgcgggtc cgaggatgtc 120cacgtcctcg ccgtcgacga cagcctcgtc gaccggaagg tcatcgagca cttgcttaag 180atctcttcct gcaaagttac ggcagtggat agtggaatca gagcccttca atttcttggc 240ctggacgagg agaaagcagc tggcgatttt aacgggttga aggtggattt gataataaca 300gactattgta tgcctggaat gactggttac gagctcctca agaaaatcaa ggaatcctct 360gctctgaggg aaattccggt ggtgatcatg tcgtccgaga acgtcttggc acgaatcgac 420aggtgtttgg aggaaggtgc ggaggacttc atcgtgaagc cggtgaagct gtcggacgtg 480aagcggttga gggatttcat gacgagagac gtcggagaaa gagtgaggag cgacggggag 540ggcaccaccc acaagaggaa gctgcaagag 570 53 1489 DNA Pinus radiata 53gcggccgcgt cgacggagga tacagggatt ggaattcctc tgcccgcaca acatcgagtt 60ttcaccccat ttatgcaggc agacagctct acatctcgaa cttatggggg tacaggaatt 120ggcctcagta ttagccgatg cttgattgaa ttaatgggcg gtgagataag atttataagc 180cgcccaggta ttggaagtac attttctttc actgctttgt tcaaagtggg ccaagcaggg 240gctgatggtg acggtgattt actacgaggt gcaagactgc cgactcattt caagggcatg 300aaggcacttg tattggatgg taacccagta tgttccctag tcacgaaata ccatttacag 360aggtttggca tagaggtgga cagcattact agttctaaag tggctttatc tatgctgaat 420ggaatggatg gttttccaac agaaggttgt agcgtaaaag atggtataga tatggtgcta 480atagagaagg atgcttgggg gtcccggcac tggcatctta tttccttcgc aagtacgagt 540aggtctcttt ccaagaggac cctttctaca gtcaaagggt ttattaaaga tgattctttt 600ggctacatcg ctgacagctg aagaaactca gaaagctaaa gctgcagggt ttgcagagac 660agttattcta aagcctttac gtgctagcat gttggctgtt tgtcttcagc tagctcttgg 720attttgcaac aggagagagc atctaagaga accttcgaag acctcctctc ctctaagtaa 780tgtattgtct ggaaaatcca tacttgtggt agatgacaac atcgtcaatc gtcgagtcgc 840tgctggtgca ctgaagaagt atggtgctaa tgttatttgc acagacagtg ggaaatctgc 900aatatccatg cttcgacaac cacacaattt caatgcatgt ttcatggatg tgcagatgcc 960agaaatggat gggtttgaag ctacgcgaca gatcagagca gcagaacttg ctaacatgga 1020gtgtacaagc aacggcggtg aaactctggc taccaacaat agatggcatg tgccaatctt 1080agcaatgacg gccgatgtaa tacaggcaac ccatgaggaa tgcctgcgat gtggcatgga 1140tgggtatgtc tcaaaacctt ttgaagaaga acaactatac aaggccctag ctccattttt 1200tgaggagtca ttatttagat attgtttaaa tattggcttt cagtgtttaa ggatttcata 1260ttccccgtgt ataatatata gaataggatc caacgattcg gtttttgtct gaaagttaga 1320cctccttgct tgaggccttg ttatggcatg tagattgatt gcaagcatgt agagttttat 1380atgctgcggg tgaagtccgt gtaagatcaa tatttgtaaa tttgaagtta gggctgaatg 1440gaaatttaaa ggcatttgct gtgcgggtat ttgcattaaa aaaaaaaaa 1489 54 370 DNA Pinus radiata 54gtcaaagggt ttattaaaga ttattctttt ggctacatcg ctgacagctg aagaaactca 60gaaagctaaa gctgcaggtt ttgcagagac agttattctg aagcctttac gtgctagcgt 120gttcgctgtt cgtctgcagc tagctcttgg attttgctac agaagagagc atctaagaga 180acctttgaag acctcctctc ctttaagtaa tgtattgtct ggaaaaggca tacttgtcgt 240agatgacaac attgtcaatc gtcgagtggc tgctggtgca ctgaagaagt atggtgccaa 300tgttatttgc acagacggtg ggaaatctgc aatttccatg cttcgacaac cacacaattt 360aatcactagt 370 55 398 DNA Eucalyptus grandis 55ggtaatcgaa agagagacgg aaaatacagc aaaaggaaga gcacgagttt cgcttcaact 60atttctaccg gcgcttcttc agcaagtggt atggcgagca acggcttggt ctcgccgcgg 120aggaggtcgt cggaccagtt tgacggcagc gacccgtctc cgtgcggttc cgaggaggtc 180cacgtcctcg ccgtcgacga cagcctcgtc gatcggaaag tcatcgagcg cttgcttaag 240atcacttcct gcaaagtgac agcagtggat agtggactca gagcccttcg atatcttggt 300ctggatgagg agaaaacggc tggagatttt aacgggttga aggtggatat gataattacc 360gactattgta tgcccggaat gactggttac gagctcct 398 56 438 DNA Eucalyptus grandis 56tgttctttca ccctttcctc ctcctccccg ttgttcctcg ccgttgttct tggtcgatcg 60ccgatcgaaa tgggtgtcac cgccgcgtcg caatttcatg tcttggcggt tgatgacagt 120ctcatcgaca ggaagctgat tgagaggctc ctcaaaacct cctcttatca agttactgca 180gttgattctg gaagtaaggc tctggagttt ctgggcctga atgagcagca gcccagaaat 240gccaacgcta cctctgtctc tccaagctat catcatcagg agatagaagt gaatttgatc 300atcacagact atttcatgcc agaaatgaca ggatatgatc tcttgagaaa gatcaaggaa 360tcgaatagtt acaaggacgt accagttgtg atcatgtcgt ctgagaatgt tccctcaaga 420atcagccaat gtttggaa 438 57 1280 DNA Eucalyptus grandis 57gggaagttgg ctgtctatgt ctctgatcgg gtaccggaag ctgttattgg tgatcctggt 60cgattccggc agataattac aaatcttgtt gggaactcaa tcaagtttac acatgaaggg 120catatatttg tctcagttca tctgctagaa gaaggatgca gtcagcatga ttttagagat 180gtggagaaga gactaagttc aaacttagtt gaagatacat cggacaaaac ttttaataca 240ttgagtgggt ttcaagtggt tgacagaagg aaaagctggg agcgctttaa gaagttaaat 300cggtccgatc aaattgatgt gaatgaatca gttgaagtac ttgttactgt tgaggataca 360ggtgttggaa ttgccagaga ggcacaaagc cgcatattca caccttttgt gcaggctgat 420agctccacat cacgtacata tggtggcact ggaattggct tgagtattag caaatgtcta 480gtggatctta tgcacgggga gatcgggttt gtaagtgaac cgggcactgg aagtacattt 540tcctttactg taccctttgc aaaatgtgaa atgaactgtc ttgaagtgaa ggggcaaaat 600tacgattcaa tcatatcaga gttcagagga ttgagagcct tggtgataga taaaagacac 660atccgagctg aggtcgcaag atatcatctt gagagactaa gaatatcagt ggacgtcgct 720tgcagtttga agtcagcctg tacttacctt tccaactctt ctagcccaag ggaactatcg 780gattttgaca tggttctcat cgacaaagat gtttgggaca ggcaaacagg tttagaactc 840aatatttcac tttggaaaca caggcaaaat ggcagcaatg gagtgtcaat acgtcccaag 900atttttcttc tggctacatc cattagtcca attgagcaca gcgagctcaa attagccaac 960ctggtagata atgtgctggc aaagcctctc agattgagtg tcttgatatc ctttctccag 1020gaagccctcg gtaatggtaa gaagaggcta tctgatagga gaaaagtatc aactcttggc 1080agtttgctga aaggaagaag aatcttggtt gtggatgaca acttggtaaa cagaagagta 1140gcagagggtg ctttaaagaa atatggtgcc attgttacct gtgttgggag cggtaaggat 1200gctgtggcca agcttcagcc gccccatgac ttcgctgctt gcttcatgga tttgcaaatg 1260ccagagatgg acgggtttgc 1280 58 582 DNA Eucalyptus grandis 58tcagcttcct cttcctcttc cccttataac cagacatcaa aaccccccca tttcctttct 60tttcaaaact caccaggcca cccacagttc tttcccactt cagactctct ctctctctac 120atctgcctct ggataatttt ccgttttcca cagaaagggt ccttttgttt cttgtcccta 180gttgttcata catataggtt tgtatctctg ggaagcagag atttctggag gatcttttgt 240tggccttcac ttttgattga tgatggagag cagcaaaggc ttctcttctc ctcggagcaa 300cgggttcccc gccggattgc gcgtccttgt ggtcgatgac gaccccactt ggttgaagat 360cctcgaaaag atgctcaaga aatgttctta tgaagttacc acatgtggtt tagcaagaga 420cgccctgaaa ctgcttcggg aaaggaaggg tggatatgac attgttatta gcgatgttaa 480catgccagac atggatggct tcaaactcct agaacttgtt ggcctcgaga tggatcttcc 540tgtaataatg atgtctgtgg atggggagac gagcagagtt aa 582 59 534 DNA Pinus radiata 59gctgaaactt gatagaagct gcagagttac acgagttgta tgattagtca gcattattga 60aggaacggca ccttttggtt cttgcttcga gattttggag cccggattcc cgatgtacac 120aatagcgcat gaaagtaaat acgcaactgc ataattttcc cttcagcagc catggttacc 180tcacgaatgt cttcggcgat gagaatgaag aaggaaaaaa atgctgcatg tggtgagcat 240ggggatgaac tggttcgatg cgacgaaatg catgtattgg cggttgatga ttgcctgata 300gaacggaagg tcattgaaaa gcttttgaag actaactttt tcaaagttac ctctgtagat 360agtgccgaaa gagcgctaga agtcttgggg tttcatgaag agcagtcgac atgtgcgacc 420actaacgcgt tcaaggttaa tatgatcatt accgattatt gtatgccagg aatgacgggc 480tatgatctgc tgaagaaagt caaggaaacc aaatgtctga aggagattcc tggt 534 60 584 DNA Pinus radiata 60caggaatgga tggatttgaa actacaaaaa gaatttgaca gatggaacaa agtccaagct 60aggaattcct actgggacag tcatcctcag agccacgtga aaatatctcg aatttccacc 120tactgatctt ggccatgact gcagatgtga ttcacgctac tcatgaataa tgcacaaagt 180atggaatgga cggatatgtt tcaaagccct ttgaagcgga acaacttttt agagaagttt 240cgctcctttt cctggtcatc actgaatcaa aacttgtaga gaggccacca tagatggtta 300attgttttgg tgagcaaaag tcttggttgt ttaggcactt tggcagcctt actggtgaac 360tttcgaattc tttaacaaga gagttttctc tggcctctcc ttagctacac atttcttgat 420tctcttgctt ttagagcagt tggatttcct tttgatagat gcaattgaaa ttcgctgaca 480tatctcatgt gaaaccaaag ggattttctt cggaaaccat atacataaag aagctaaggt 540cacctgctgg agttgaattt tcaaaaaaaa aaaaaaaaaa aaaa 584 61 702 DNA Pinus radiata 61agaagggctg tttcagtttg agttgccctt gaacaaaatc ttctatgaat ttattcaaga 60aaagattcaa gccagcaatg cttgggtttg agctgaaaaa aacagagaca gagacagaag 120aactaaattt attagaaaga aaaaactaaa attactaaca ggtggataca gatgcagagg 180gttaaagtaa tgaagaagat aaagagaggg tgattcaatt tgtttgtaga agccgacgca 240gggcttcaac aacctcaacc tcaagacgac tacgggtttc tttcatggtt ggttggagaa 300cgaagagcaa agcaccagaa ttagaagaag aaggatgttt agggttcaaa gagggtttgg 360attaggtttc accaaccgag gtcgatctat ctcaagtatc tatgatggtt cccatcagat 420agaagcttcc aagattttat acgagcttaa aacagtgaat tcaagcgaag gaaaaaagtt 480catgcaaata aatggaaaac aggtggaggg aggagggtga tacgaattta atgaacaata 540gaacatcaat gagccataag gcaaggtcga aggaagcata agagcagagc cataaggcaa 600ggttgtagga agcattagag cagaacaaag cacaacatag aggaaataaa tacctggaat 660tagaagaaaa gcagccctgt cggtacatca aattgtctta cg 702 62 600 DNA Pinus radiata 62gtcgacagac aggcaaagca aggaaggagg acagagggca gatggtttct gatgtcaatt 60aatgagacag gcacagtaag gaaggaggac agagagaggg gttcatgtga aacccaacgg 120acacagaagg aagaaggaca cagaaggaat aagggctgct tcagtttgag ttgccgttga 180acaaaatctt cgatgagttt attcaagaat agattcaagc aagcaatgct tgggtttgag 240cagagaaaaa acagagacag agacagaaag aactaaattt attagaaaga aaaagctaaa 300attaataaca ggtggataca gatgcagggg gttaaagtaa tgaagaggat aaagagaggg 360tgattcaatt tgttggcagg agccgacgca gggcttcaac aagctcaacc tcaagacgac 420tacgggtttc tttcatggtt ggttgaagaa cgaagaagag caaagcacca gaattacaag 480aaggaggatg tttagggttc aaagagggtt tggattgggt ttcaccaacc gaggtcgatc 540tatctatcat ggttcccatc agatagaagc ttccaagatt ttatacaagc ttaaaacagt 600 63 1656 DNA Pinus radiata 63gcggccgcgt cgacctttat aaaccatatt ataagactga gtaatgtgac aattaccatt 60accatatacc ccacagaaga agagagtcag tatttgggtt cccagcttct gcacaatctg 120taatattttg ggtttattga gatggctgta tctcaacatc tgtttttgag tgctcaacgc 180ctaaatggga gagaggatga aggcagtctg taccttttga gagcaggggt gaactgggac 240ctgctgataa tgggtgttgc tctggtggcc tgtttggcta ttttagggat ggtttggaag 300cgtaggagga catggtctta ttgtgagggt ttgcaggaag aagatgcagg ccagagagct 360caggaaaccc aatgctctaa aggattcatg accaacgtgt ttcacaatac aagagattgc 420agatcagaac agattatttg ggatgatatc cacatttcat ctcagactga aaccagaagt 480cagaaagtta gaactgtgaa gtcaaaaagt tccatgattt ctcgggattc ttgtagctcc 540cccagacgaa tcttgctcgt ggaagacaca caaatcaaca gaataatttt cgggagggtg 600cttcaaagcc ttaatcttta ctgtgaagaa gctgagaatg ggaaagtagc agtggactat 660ttcaagcagg gcagaacata tgatcttgtg ttaatggaca aagagatgcc tgttatggat 720gggcatgagg caacaaggca actgagatca atgggagtca agacacccat tgttgcactt 780acagcaaata ccttgcaatc tgataaagac ctcttctttg aggctggtgt tgatgatttt 840caatcaaagc ctctgtccag agacagactt gtacaattac tagatcaata tggtgtggat 900ggttgtgctg gcaacagaag gggttgaaag agtttcaagg tttcattgta ttatacccat 960ggcttcgtta tgaaaaaaaa cagtgcagat tgcaggttgc ctgctttgta agtcttgatc 1020tgcattattg cagtgatttg attaagccac gaggaatatg gttttagggt ttccagacat 1080ttgcaatcct gcaatcctcc tgagcaacac tgaactttcc tacatcttgg ggaaggcagg 1140taggctagag ctggaagtaa aatgtgaaca agttgtgaaa agtgcataaa ctggtataaa 1200gcaaaacaat tgattttttt caagcagcag tgtcaaagct gaagttgcag cactattaaa 1260aagctggaaa tgaatcttgg tggggagaga aagaatttga agttgcagca ctattaaaag 1320gctggaaatg aatcttggtg ggggagagaa cgattctttc tctcgactat ccaagttata 1380ctcaattgat cttcatcttc tgtgaaggac tttggtcaca ggaattcctt ctcaagatca 1440atcaaaacgg ttttgagcca cacaaattgg caacaggctg ggatcaacaa gaagacttat 1500gagagagctt ttagtttttt cagtggagcc gaacagtggg actttgggaa atgcttgttc 1560aaagttctta actccttcgc ctatatgggt ggcctgcaca atttgtatct ataataagga 1620tttcttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1656 64 360 DNA Pinus radiata 64gggtatttgg gttctacgtc ttctgtgcaa cctgttaatt tctatctgtt ttggtttatt 60gaggtggctg tacctcaaca tctggttttg ggtcatcaat acctaagtgg gacagaggga 120ggagacggtc tgtactttct gagagcaggg ttgaactggg tccttctgat aatgggtgtt 180gccctggtgg cctgtttggc tatttttggg atggtttgga agcgtaggag gacatggtct 240tattgtgggg ctatgcagaa agaggatgca agccagagag ctcaggaggc ccaatgctct 300aaaggatgca tgaccaatgt gttgccgaat acgagagctt gtagaggagc acacattata 360 65 466 DNA Pinus radiata 65aacagattgc agatcagcac gcattatttg ggatgatatc cacatttcat ctcagactga 60aaccacaagt cagaaagtta gaactgtaaa gtcaaaaagt tccatgattt ctcaggattc 120ttgtagctcc cccagacgaa tcttgctcgt ggaagacaca caaatcaaca gagtaagtta 180taccaggact gattcgttgt ttggttttag gatgctgctt gtggccatat aaggttaatt 240ttcctggagc ttgctgctga ggtctcattc attattttac actgaatcaa cctcagataa 300ttttcgggag ggtgcttcaa agccttaatc tttactgtga agaagctgag aatggaaagg 360tagcagtgga ctatttcaag cagggcagaa catatgatct tgtattaatg gacaaagaga 420tgcctgttat ggatgggcat gaggtatgca actgttaaat ctgtaa 466 66 642 DNA Pinus radiata 66aagcgatttc tggagggcca cgagctgtct tatctccgag ccatcggtgt tattattctg 60tccgctgtgc tgaagcggcg catgatcctg gcggacaagg cgaagagtct cttcatctcc 120aacatatcac acgagttacg gacaccgctg catgggattc tcgcagcggc ggaactgctc 180ggcgatagcc cgttaaatca ctcgcagctc tccttcctcg agactgtaca ggcgtgtggg 240acttcgcttg tcgagacggt aaatcacgtg ttagatttca ccaagctgag cgggaactcc 300aaggcgggcg gggtggagaa ggtgatcgtg ccgaccaggg tggatctgat gcagctcatc 360gaggaggcgg tggatgggtg ctggattgga catcgggccc ggacagcgat catgggcgac 420acgggcatcg gaagcgtgta ttcgccaccg gaggatttat cttctcccaa gcagctcgtt 480gagaccgtcg tcgacattgg atggcgcaaa aagggatggt cgctcaagtg cgagaaaggc 540gggatccggc gggtgctgat gaatgtgttt gggaacagcc tgaagtttac tactaacgga 600tacgtgcacg taattctgcg tgagctgcct cggagcggcg at 642 67 909 DNA Eucalyptus grandis 67gttgaaaact cctcaacaag aaatcgtgcg ttcgtacagt ttcatctaca tcaggaaagg 60ctctttgctc tgatcttttc cttgcgagtt aatcattatg acgatggccg gcgaaattct 120ccggcggcag tcgccggcgg aggtcgactt gtgtggtggg tcgggtcagg agctgcatgt 180tcttgcggtg gatgatagcc ttgtggatag gaaggtcatt gagaagctgc ttaagagatt 240gtcttgtaaa gttacggctg ttgatagtgg attgagggct ttgcagtttc tgggattgga 300tggagagaag agctctgttg gacttgatga tttgaaggtt aatctgataa tgactgatta 360ctccatgccg ggcatgactg gatatgagct tctcaagaag atcaaggaat catcagcttt 420cagagaaacg ccggtggtga tcatgtcctc cgaaagaatc ctcgcccgta ttaaccgatg 480tctagaggaa ggagcagagg aatttcttgc gaagccagtg caactgtccg atgttcagcg 540cctgaaaaat ttcgtgatgg gtgggggaga agtttgcccg gacagaagaa tcaacaagag 600aagactcgaa gaaaataacg ataacgacga caacgaaaat catgccccat cgccggtgtc 660gcccctgtgc agtcgcgatt gggcagtgtg ctcatcttca tcgtccgatt cttcatcgcc 720atctatagcc gtgtcttcat cgaagaggct taagatacat catcaagctt gaggatattc 780attcgtatat accagaattt gatttattgt tgttttttgg ccgatgatcg gcatattcat 840ggctaggaat ggcgcacctt ttgttcagta aatataatat gatctctttc ccctaaaaaa 900aaaaaaaaa 909 68 666 PRT Pinus radiata 68Met Glu Arg Leu Glu Met Ala Met Phe Leu Met Leu Leu Ile Ile Phe 1 5 10 15Phe Phe Arg Asp Cys Glu Ala Gln Gly Lys Ser Asp Tyr His Ala Leu 20 25 30Ile Ala Phe Lys Ala Ser Ser Asp Ile Asn Asn Thr Leu Ala Phe Ser 35 40 45Trp Thr His Lys Asn Pro Cys Arg Arg Lys Trp Tyr Gly Val Gly Cys 50 55 60Glu Asn Gly Arg Val Val Arg Leu Val Leu Gln Asp Leu Asp Leu Val65 70 75 80Gly Pro Ile Asp Ala Leu Thr Gly Leu His Glu Leu Arg Ile Leu Ser 85 90 95Leu Lys Arg Asn Ser Leu Asn Gly Thr Ile Pro Asp Phe Leu Asn Trp 100 105 110Arg Ser Leu Lys Phe Leu Phe Leu Ser Asp Asn Tyr Phe Ser Gly Pro 115 120 125Leu Pro Ser Thr Ile Ala Ser Leu Asp His Leu Leu Arg Leu Asp Val 130 135 140Ser Asn Asn His Leu Gly Gly Gln Ile Pro Leu Ser Ile Thr Ser Met145 150 155 160Thr His Leu Leu Thr Leu Arg Leu Glu Asn Asn Glu Phe Ser Gly Ser 165 170 175Ile Ser Asp Leu Met Leu Pro Asn Thr Val Glu Glu Phe Asn Val Ser 180 185 190Asp Asn Arg Leu Ser Gly Met Ile Pro Ala Ser Leu Ser Arg Phe Pro 195 200 205Ser Ser Gly Phe Val Asn Asn Glu Glu Leu Cys Gly Ser Pro Leu Gln 210 215 220Ser Cys Asn Glu Ser Asn Asn Ala Ser Ser Ala Ser Asn Pro Tyr Met225 230 235 240Ala Ser Ser Pro Ser Met Ile Ser Gly Ser Leu Pro Val His Arg Asn 245 250 255Asn Ser Ser Thr Gln Leu Ser Lys Gly Asp Ile Ile Ala Ile Val Val 260 265 270Gly Asp Val Ala Val Leu Ser Leu Ile Gly Cys Val Ile Phe Cys Tyr 275 280 285Tyr Trp Lys Lys Lys Gly Val Lys Gln Lys Lys Pro Lys Pro Lys Pro 290 295 300Ala Gln Arg Cys Pro Thr Asp Arg Leu Ala Val His Ser Ser Asp Gln305 310 315 320Cys Pro Asn Asn Gln Ser Ile Thr Ala Gly Lys Cys Lys Leu Ile Phe 325 330 335Phe Asp Asp Gly Arg Pro Phe Glu Leu Glu His Leu Leu Arg Ala Ser 340 345 350Ala Glu Met Leu Gly Lys Gly Asn Phe Gly Ser Ala Tyr Lys Ala Ile 355 360 365Met Glu Asp Gly Ser Val Val Ala Val Lys Arg Leu Lys Asp Leu Tyr 370 375 380Gly Ile Gly Arg Lys Glu Phe Glu Gln His Met Glu Leu Met Gly Ser385 390 395 400Leu Arg His Gln Asn Val Val Asn Leu Arg Ala Tyr Tyr Phe Ala Arg 405 410 415Asp Glu Lys Leu Leu Val Tyr Asp Tyr Met Pro Asn Gly Ser Leu Tyr 420 425 430Ala Leu Leu His Gly Ser Arg Gly Pro Gly Arg Thr Pro Leu Asp Trp 435 440 445Thr Thr Arg Met Lys Ile Ala Leu Gly Ala Ala Lys Gly Leu Ala Phe 450 455 460Ile His Ser His Cys Lys Ser Pro Lys Ile Gly His Gly Asn Ile Lys465 470 475 480Ser Ser Asn Ile Leu Leu Asp Arg Asn Gly Asn Ala Cys Ile Ser Asp 485 490 495Phe Gly Leu Ala Leu Leu Val Ser Pro Ser Val Ala Ala Ser Arg Met 500 505 510Val Gly Tyr Thr Ala Pro Glu Gln Ala Ala Thr Lys Lys Ile Ser Gln 515 520 525Lys Ala Asp Val Tyr Ser Phe Gly Val Leu Leu Leu Glu Met Leu Thr 530 535 540Gly Lys Ala Pro Val Gln Ala His Met Gln Glu Asp Tyr His Ser Ala545 550 555 560Ile Asp Leu Pro Arg Trp Val Gln Ser Ile Val Pro Glu Glu Trp Thr 565 570 575Ser Glu Val Phe Asp Ile Glu Leu Met Arg Phe Lys Asn Ile Glu Glu 580 585 590Glu Leu Val Ser Met Leu Gln Ile Ala Leu Leu Cys Ala Ser Gln Ser 595 600 605Pro Gln Gln Arg Pro Lys Met Ser His Val Val Arg Val Ile Gln Asp 610 615 620Ile Arg Gly Asp His His Ser Pro Ser Met Gln Asn Ser Leu Ser Gln625 630 635 640Ser Pro Ser Met Gln Glu Pro Gly His Ser Ile Ser Asp Ser Pro Ser 645 650 655Val Ser Glu Asp Ser Gly Ile Arg Gly Leu 660 665 69 690 PRT Eucalyptus grandis 69Met Gly Phe Ala Ser Leu Arg Ser Gln Cys Leu Phe Phe Phe Leu Leu 1 5 10 15Trp Val Phe Ile Phe Phe Ala Ser Arg Ser Asp Val Leu Val Ser Ala 20 25 30Ala Thr Ser Ala Glu Leu Arg Ala Leu Met Asp Met Lys Ala Ser Leu 35 40 45Asp Pro Glu Ser Arg Tyr Leu Ser Ser Trp Thr Val Asp Gly Asp Pro 50 55 60Cys Asp Gly Ser Phe Gln Gly Val Cys Cys Asp Asp Glu Gly Arg Val65 70 75 80Ala Asn Val Ser Leu Gln Gly Arg Arg Leu Thr Gly Arg Leu Ser Pro 85 90 95Ala Ile Ala Gly Leu Thr His Leu Thr Gly Leu Tyr Leu His Tyr Asn 100 105 110Ser Leu Cys Gly Glu Ile Pro Arg Glu Leu Gly Asn Leu Ser Ala Leu 115 120 125Ser Asp Leu Tyr Leu Asn Met Asn Asn Leu Ser Gly Gln Ile Pro Pro 130 135 140Glu Met Ala Asp Val Ala Ser Leu Gln Val Met Gln Leu Ser Tyr Asn145 150 155 160Gln Leu Thr Gly Ser Ile Pro Thr Lys Leu Gly Ser Leu Lys Lys Leu 165 170 175Ser Val Leu Ala Leu Gln Ser Asn Gln Leu Thr Gly Ala Ile Pro Ala 180 185 190Ser Leu Gly Asp Leu Gly Thr Leu Thr Arg Leu Tyr Leu Ser Phe Asn 195 200 205Arg Leu Phe Gly Ser Ile Pro Met Lys Ile Ala Asp Leu Pro Leu Leu 210 215 220Glu Val Leu Asp Val Gln Asn Asn Thr Leu Ser Gly Asn Val Pro Pro225 230 235 240Ala Leu Lys Arg Leu Asn Glu Gly Phe Leu Tyr Glu Asn Asn Phe Asp 245 250 255Leu Cys Gly Thr Gly Phe Leu Ser Leu Arg Thr Cys Asn Ala Leu Glu 260 265 270Gly Arg Lys Pro Ser Gln Pro Gln Pro Tyr Gly Ala Ala Thr Thr Val 275 280 285Pro Ser Thr Ser Ile Pro Glu Thr Ala Asn Val Val Leu Pro Cys Asn 290 295 300Leu Thr Glu Cys Ser Ser Leu Pro Lys Ser Ala His Pro Ser Ala Leu305 310 315 320Ile Gly Ser Ile Leu Ala Thr Val Ala Leu Ser Ala Ile Gly Phe Leu 325 330 335Leu Phe Thr His Tyr Arg Arg Arg Lys Gln Lys Leu Gly Phe Ser Ala 340 345 350Glu Val Cys Asp Gly His Leu Ser Thr Asp Gln Pro Lys Ser Ala Tyr 355 360 365Lys Lys Asn Gly Ser Pro Leu Ala Ser Leu Glu Tyr Ser Asn Gly Trp 370 375 380Asp Pro Leu Ala Asp Ala Arg Ile Phe Asn Glu Phe Ser Glu Glu Ala385 390 395 400Phe Gln Ser Phe Arg Phe Asn Leu Glu Glu Val Glu Ser Ala Thr Gln 405 410 415Tyr Phe Ser Glu Leu Asn Val Leu Gly Lys Ser Asn Phe Ser Thr Thr 420 425 430Tyr Arg Gly Ile Leu Arg Asp Gly Ser Val Val Ser Ile Lys Cys Ile 435 440 445Asn Lys Thr Ser Cys Lys Ala Asp Glu Ser Glu Phe Leu Lys Gly Leu 450 455 460Asn Met Leu Thr Ser Leu Arg His Glu Asn Leu Val Arg Leu Arg Gly465 470 475 480Phe Cys Cys Ser Thr Ala Arg Gly Glu Cys Phe Leu Ile Tyr Asp Tyr 485 490 495Val Pro Asn Gly Thr Leu Leu Ser Phe Leu Asp Leu Glu Glu Gly Asp 500 505 510Ser Gly Thr Leu Glu Trp Ser Thr Arg Val Ser Ile Val Lys Gly Ile 515 520 525Ala Lys Gly Ile Ala Tyr Leu His Ala His Lys Pro Asn Lys Ala Pro 530 535 540Leu Leu His Gln Asn Ile Ser Ala Asp Lys Val Leu Ile Asp Gln Arg545 550 555 560Phe Asn Pro Leu Leu Tyr Gln Ser Gly Leu His Arg Leu Leu Thr Asn 565 570 575Asp Val Val Phe Ser Leu Leu Lys Ala Ser Ala Ala Met Gly Tyr Leu 580 585 590Ala Pro Glu Tyr Met Ser Thr Gly Arg Phe Thr Glu Lys Ser Asp Val 595 600 605Tyr Ala Phe Gly Met Ile Val Phe Gln Ile Leu Ser Gly Lys Gln Lys 610 615 620Val Asp His Ser Met Arg Leu Ala Ala Glu Ser Cys Arg Phe Gln Glu625 630 635 640Phe Ile Asp Ala Asn Ile His Gly Arg Phe Phe Glu Tyr Glu Ala Ala 645 650 655Lys Leu Ala Lys Ile Ala Ser Leu Cys Thr Asn Glu Ser Pro Tyr Asp 660 665 670Arg Pro Ser Met Asp Ala Val Ile His Glu Leu Ser Asn Cys Ser Ser 675 680 685Cys Leu 690 70 655 PRT Pinus radiata 70Met Tyr Trp Ser Gln Met Gly Asn Ile Arg Gln Leu Phe Asn Gly Phe 1 5 10 15Phe Met Leu Val Leu Val Val Ala Val Val Lys Gly Glu Pro Thr Gly 20 25 30Asp Lys Gln Ala Leu Leu Asp Phe Leu Ser Lys Val Pro His Gly Arg 35 40 45Arg Leu Asn Trp Asn Ala Ser Ser Ser Ala Cys Thr Trp Val Gly Val 50 55 60Thr Cys Asn Ser Asn Lys Asp Arg Ile Trp Glu Val Arg Leu Pro Gly65 70 75 80Val Gly Leu Phe Gly Pro Ile Pro Pro Gly Thr Leu Gly Arg Leu Thr 85 90 95Glu Leu Arg Val Leu Ser Leu Arg Ser Asn Leu Leu Thr Gly Ser Leu 100 105 110Pro Ser Asp Leu Ala Asn Ala Lys Ala Leu Arg Ser Ile Tyr Leu Gln 115 120 125His Asn Leu Phe Ser Gly Pro Leu Pro Pro Phe Leu Ser Gln Trp Gly 130 135 140Arg Leu Ser Arg Leu Asp Leu Ser Phe Asn Arg Leu Asn Gly Ser Ile145 150 155 160Pro Phe Ser Leu Asn Asn Leu Thr His Leu Thr Gly Leu Leu Leu Gln 165 170 175Asn Asn Ser Leu Ser Gly Ser Ile Pro Asn Leu Asn Ile Gln Asn Leu 180 185 190Thr Leu Leu Ser Val Ala Asn Asn Gln Leu Asn Gly Ser Ile Pro Arg 195 200 205Ser Leu Gln Lys Phe Pro Lys Thr Ser Phe Gln Gly Asn Ala Gln Leu 210 215 220Cys Gly Val Pro Leu Lys Leu Cys Lys Ser Phe Phe Pro Ser Pro Ser225 230 235 240Pro Ser Pro Asn Gly Ser Ala Val Pro Arg Arg Ser Lys Lys Ser Lys 245 250 255Leu Ser Thr Gly Val Val Val Ala Ile Ile Val Gly Ala Val Ala Val 260 265 270Leu Phe Leu Leu Leu Ala Cys Leu Phe Leu Cys Cys Val Arg Lys His 275 280 285Arg Gly Glu Ser Ala Thr Glu Lys Pro Gln Lys Asp Glu Arg Thr Thr 290 295 300Val Glu Lys Gly Gly Pro Ser Lys Glu Glu Tyr Met Gly Thr Ala Gln305 310 315 320Glu Thr Glu Arg Asn Lys Leu Val Phe Phe Glu Gly Ser Gln Tyr Thr 325 330 335Phe Asp Leu Glu Asp Leu Leu Arg Ala Ser Ala Glu Val Leu Gly Lys 340 345 350Gly Ser Val Gly Thr Ala Tyr Lys Ala Val Leu Glu Asp Gly Thr Thr 355 360 365Val Val Val Lys Arg Leu Lys Asp Val Ala Val Asn Arg Arg Asp Phe 370 375 380Glu Gln Gln Met Glu Leu Val Gly Arg Ile Arg His Arg Asn Leu Val385 390 395 400Pro Leu Arg Ala Phe Tyr Phe Ser Lys Asp Glu Lys Leu Leu Val Tyr 405 410 415Asp Tyr Met Pro Ala Gly Ser Leu Ser Ala Leu Leu His Gly Ser Arg 420 425 430Gly Ser Gly Arg Thr Pro Leu Asp Trp Glu Thr Arg Met Arg Ile Ala 435 440 445Leu Gly Ala Ala Arg Gly Ile Ser His Ile His Glu Glu Gly Gly Gly 450 455 460Lys Phe Thr His Gly Asn Ile Lys Ser Ser Asn Val Leu Leu Thr Ser465 470 475 480Asp Leu Asp Gly Cys Val Ser Asp Phe Gly Leu Val Pro Leu Phe Ser 485 490 495Ala Ala Ala Ala Ala Asn Arg Ile Ala Gly Tyr Arg Ala Pro Glu Val 500 505 510Ile Glu Thr Arg Lys Val Thr Gln Lys Ser Asp Val Tyr Ser Phe Gly 515 520 525Val Leu Leu Leu Glu Leu Leu Thr Gly Lys Ala Pro Asn Gln Ala Ser 530 535 540Leu Asn Asp Glu Gly Ile Asp Leu Pro Arg Trp Val Gln Ser Val Val545 550 555 560Arg Glu Glu Trp Thr Ala Glu Val Phe Asp Val Glu Leu Met Arg Tyr 565 570 575Gln Asn Ile Glu Glu Glu Met Val Gln Leu Leu Gln Ile Ala Met Ala 580 585 590Cys Val Ala Thr Val Pro Asp Gln Arg Pro Arg Met Gln Asp Val Val 595 600 605Lys Met Ile Glu Asp Met Arg Gln Phe Glu Thr Asp Glu Gly Asn Arg 610 615 620Gln Ser Ser Asp Asp Lys Ser Lys Glu Ser Asn Gly Gln Thr Pro Pro625 630 635 640Gln Gln Ala Thr Pro Glu Ala Arg Thr Pro Thr Ala Arg Thr Pro 645 650 655 71 630 PRT Pinus radiata 71Met Gln Gln Pro Tyr Val Val Leu Ala Leu Leu Trp Met Leu Leu Leu 1 5 10 15His His Pro Leu Trp Arg Val Phe Ala Asn Thr Glu Gly Asp Ala Leu 20 25 30His Ser Leu Arg Ser Asn Leu Leu Asp Pro Asn Asn Val Leu Gln Ser 35 40 45Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe His Val Thr Cys 50 55 60Asn Asn Asp Asn Ser Val Ile Arg Val Asp Leu Gly Asn Ala Gln Leu65 70 75 80Ser Gly Ser Leu Val Pro Gln Leu Gly Leu Leu Asn Asn Leu Gln Tyr 85 90 95Leu Glu Leu Tyr Ser Asn Asn Ile Ser Gly Pro Ile Pro Ser Asp Leu 100 105 110Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr Leu Asn Asn Phe 115 120 125Thr Gly Leu Ile Pro Glu Ser Leu Gly Lys Leu Ser Arg Leu Arg Phe 130 135 140Leu Arg Leu Asn Asn Asn Ser Leu Val Gly Arg Ile Pro Met Ser Leu145 150 155 160Thr Thr Ile Thr Ala Leu Gln Val Leu Asp Leu Ser Asn Asn Asn Leu 165 170 175Thr Gly Glu Val Pro Ala Asn Gly Ser Phe Ser Leu Phe Thr Pro Ile 180 185 190Ser Phe Gly Gly Asn Gln Tyr Leu Cys Gly Pro Val Ala Gln Lys Pro 195 200 205Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro Phe Val Pro Pro 210 215 220Pro Pro Val Ala Gly Ser Asn Gly Ala Arg Val Gln Ser Ser Ser Ser225 230 235 240Thr Gly Ala Ile Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe 245 250 255Ala Ala Pro Ala Ile Gly Phe Ala Trp Trp Arg Arg Arg Lys Pro Gln 260 265 270Glu His Phe Phe Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu 275 280 285Gly Gln Leu Lys Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Thr Asp 290 295 300Gly Phe Ser Asn Arg Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val305 310 315 320Tyr Lys Gly Arg Leu Ala Asp Gly Ser Leu Val Ala Val Lys Arg Leu 325 330 335Lys Glu Glu Arg Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val 340 345 350Glu Met Ile Ser Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly 355 360 365Phe Cys Met Thr Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala 370 375 380Asn Gly Ser Val Ala Ser Cys Leu Arg Glu Arg Ala Gln Asn Asp Pro385 390 395 400Pro Leu Asp Trp Pro Thr Arg Lys Arg Ile Ala Leu Gly Ser Ala Arg 405 410 415Gly Leu Ser Tyr Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg 420 425 430Asp Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Glu Tyr Glu Ala Val 435 440 445Val Gly Asp Phe Gly Leu Ala Lys Leu Met Asp Tyr Lys Asp Thr His 450 455 460Val Thr Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr465 470 475 480Leu Ser Thr Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly 485 490 495Ile Met Leu Leu Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala 500 505 510Arg Leu Ala Asn Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly 515 520 525Leu Leu Lys Glu Arg Arg Leu Asp Met Leu Val Asp Pro Asp Leu Lys 530 535 540Asn Asn Tyr Val Glu Ala Glu Val Glu Gln Leu Ile Gln Val Ala Leu545 550 555 560Leu Cys Thr Gln Gly Ser Pro Met Asp Arg Pro Lys Met Ser Glu Val 565 570 575Val Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Glu Trp 580 585 590Gln Lys Val Glu Val Val Arg Ser Gln Glu Val Glu Leu Val Pro His 595 600 605Arg Asn Ser Glu Trp Ile Val Asp Ser Thr Asp Asn Leu His Ala Val 610 615 620Glu Leu Ser Gly Pro Arg625 630 72 910 PRT Pinus radiata 72Met Leu Leu Leu Ala Thr Leu Ser Phe Ile Leu Phe Leu Asn Pro Phe 1 5 10 15Ala Phe Ser Thr Pro Ile Ala His Phe Pro His His Pro Pro Arg Leu 20 25 30Thr Asn Ala Ser Asp Gln His Ala Leu Leu Ala Phe Lys Ser Ala Ile 35 40 45Thr Tyr Asp Pro Ser Gln Ser Leu Ala Thr Ser Trp Leu Pro Asn Val 50 55 60Ser Phe Cys Gln Trp Thr Gly Ile Ile Cys Ser Arg Arg Arg Gln Arg65 70 75 80Val Ile Ser Leu Asn Val Ser Ser Met Gly Leu Gln Gly Thr Ile Ser 85 90 95Pro Leu Leu Ala Asn Leu Ser Phe Leu Thr Val Leu Asp Leu His Asn 100 105 110Asn Ser Phe Asp Cys His Ile Pro Tyr Gln Leu Gly Thr Leu Phe Arg 115 120 125Leu Lys Met Leu Arg Leu Ser Lys Asn Gln Leu Gln Gly Ser Ile Pro 130 135 140Pro Thr Leu Ala Asn Cys Arg Ser Leu Arg Asn Leu Thr Leu Ser Phe145 150 155 160Asn Asn Leu Thr Gly Asn Ile Pro Pro Gln Leu Cys Leu Leu Pro Asn 165 170 175Leu Ile Cys Met Ser Leu Gly Ile Asn Asn Leu Thr Gly Thr Ile Pro 180 185 190Asp Cys Leu Gly Asn Ile Ser Ser Leu Gln Tyr Leu Ser Leu Ser Gln 195 200 205Gly Asn Leu Gln Gly Ser Val Pro Ser Glu Leu Gly Arg Leu Ser Gln 210 215 220Leu Ile Val Leu Asp Leu Phe Gly Asn His Leu Thr Gly Cys Ile Pro225 230 235 240Ser Ser Leu Ser Asn Cys Thr Asn Leu Glu Leu Leu Asp Ile Gly Asp 245 250 255Asn Gln Leu Val Gly His Ile Pro Ser His Leu Cys Thr Lys Lys Thr 260 265 270Thr Gln Leu Met Tyr Leu Arg Leu Gly Ala Asn Gln Leu Ser Gly Ser 275 280 285Val Pro Ser Ser Leu Phe Asn Cys Thr Lys Leu Gln Glu Ile Ala Leu 290 295 300Pro Tyr Asn Gln Leu Ser Gly Ile Val Pro Met Glu Leu Gly Lys Leu305 310 315 320Thr His Leu Gln Arg Leu Phe Phe Gly Gly Asn Tyr Phe Ile Ser Gly 325 330 335Asn Thr Met Arg Cys Pro Ile Leu Thr Ala Leu Ser Asn Cys Ser Asp 340 345 350Leu Gln Tyr Val Asp Leu Ser Glu Asn Asn Phe Thr Gly Gln Leu Pro 355 360 365Phe Ser Ile Gly His Leu Ser Lys Lys Leu Tyr His Leu Asp Leu Gly 370 375 380Ser Asn Glu Leu Ala Gly Glu Ile Pro Pro Ala Ile Gly Asn Leu Ser385 390 395 400Ser Leu Thr Phe Leu Asn Leu Gly Arg Asn Tyr Phe Thr Gly Ser Ile 405 410 415Pro Ser Ser Leu Ile Met Leu Gln Lys Leu Glu Arg Leu Tyr Met Asp 420 425 430Ser Asn Asn Leu Gln Gly Asn Ile Pro Met Glu Ile Gly Gln Leu Lys 435 440 445Ser Leu Gly Leu Leu Tyr Leu Ser Gly Asn Asn Leu Ser Gly Lys Ile 450 455 460Pro Asp Phe Val Ala Asn Leu Gln Gln Leu Arg Tyr Leu Tyr Leu Asn465 470 475 480His Asn Gln Leu Ser Gly Asp Ile Asn Ala Asn Leu Gly Lys Cys Val 485 490 495Asn Leu Leu Leu Leu Asp Leu Ser Tyr Asn Lys Leu Ser Gly His Ile 500 505 510Pro Gln Glu Leu Ala Gly Leu Ala Asn Leu Ala Phe Tyr Phe Asn Leu 515 520 525Ser Asn Asn Leu Leu Ser Gly His Val Pro Leu Glu Leu Gly Lys Phe 530 535 540Asp Met Leu Gln Ala Ile Asp Ile Ser Ala Asn Gln Ile Thr Gly Tyr545 550 555 560Ile Pro Ser Ile Val Gly Ser Trp Lys Glu Val Ala Tyr Leu Asn Leu 565 570 575Ser Tyr Asn Ala Leu Glu Gly Pro Ile Pro Val Ser Ile Ser Glu Leu 580 585 590Leu Ser Leu Gln Asp Leu Asp Leu Ser Ser Asn Asn Leu Ser Gly Gly 595 600 605Ile Pro Ile Ser Leu Ala Asn Leu Thr Met Leu His His Leu Asn Phe 610 615 620Ser Phe Asn Lys Leu Ser Gly Glu Val Pro Lys Glu Gly Val Phe Lys625 630 635 640Asn Ile Gly Ala Thr Ala Phe Met Gly Asn Leu Gly Leu Cys Gly Pro 645 650 655Trp Val Asn Leu Pro Pro Cys Tyr Ala His Lys His Lys Ser Val Leu 660 665 670Asn Leu Lys Arg Val Ile Ile Leu Val Val Val Val Ala Ile Val Val 675 680 685Leu Cys Leu Phe Leu Ala Ile Leu Trp Arg Lys Asn Cys Arg Arg Asn 690 695 700Ile Gln Arg Asp Ile Gly Pro Ser Leu Asn Val Gly His Arg Arg Ile705 710 715 720Ser Tyr Ala Glu Leu Val Ile Ala Thr Asn Glu Phe Ser Asp Ala Asn 725 730 735Leu Leu Gly Ile Gly Ser Phe Gly Lys Val Tyr Lys Gly Ile Leu Asn 740 745 750Asp Gly Thr Met Val Ala Val Lys Leu Leu Asn Leu Gln Asn Glu Gly 755 760 765Ala Gln Lys Ser Phe Asp Arg Glu Cys Lys Val Leu Gly Arg Val Arg 770 775 780His Arg Asn Leu Ile Arg Val Ile Thr Cys Tyr Ser Asp Leu Gln Ile785 790 795 800Lys Ala Leu Ile Phe Pro Leu Met Pro Lys Gly Ser Leu Glu Lys Trp 805 810 815Leu Tyr Pro Asp Asp Gly Glu Gln Ser Cys Leu Asn Leu Ile Gln Arg 820 825 830Leu Asn Ile Ala Ile Asp Ile Ala Gln Gly Met Thr Tyr Leu His His 835 840 845His Cys Phe Val Gln Val Ile His Cys Asp Leu Lys Pro Asn Asn Val 850 855 860Leu Leu Gly Glu Asp Met Thr Ala Tyr Leu Ile Asp Phe Gly Ile Ala865 870 875 880Thr Ile Cys Phe Ala Asn Asn Glu Asp Gly Ala Leu Thr Ser Thr Asn 885 890 895Ala Leu Lys Gly Ser Thr Gly Tyr Ile Pro Pro Gly Ile Ile 900 905 910 73 947 PRT Pinus radiata 73Met Arg Tyr Ser Trp Thr Cys Ser Pro Phe Leu Ser Leu Leu Phe Ile 1 5 10 15Leu Ser Cys Leu Asp Ser Gly Ile Cys Leu Asp Asn Gln Gln Thr Gln 20 25 30Val Met Lys Lys Leu Ser Ser Tyr Thr Pro Ser Trp Thr Thr Val Lys 35 40 45Ser Asp Asn Pro Cys Gly Trp Ser Gly Val Asn Cys Thr Ala Glu Glu 50 55 60Ser Asn Val Thr Glu Leu His Met Ser Gly Phe Arg Met Lys Gly Asn65 70 75 80Ala Trp Gln Thr Ile Cys Lys Leu Gln Ala Leu Gln Val Leu Asp Val 85 90 95Ser Asp Asn Leu Leu Ser Thr Pro Ser Asp Asn Asp Ile Gln Ala Cys 100 105 110Thr Asn Leu Phe Ser Leu Asn Ile Ser Ser Asn Phe Leu Pro Gly Ser 115 120 125Ser Leu Pro Ser Leu Ala Pro Met Arg Lys Leu His Phe Leu Asp Val 130 135 140Ser His Asn Gly Phe Ala Gly Glu Phe Gly Pro Gln Ile Gln His Leu145 150 155 160Thr Asp Leu Arg Val Leu Asn Leu Thr Tyr Asn Asn Phe Ser Gly Pro 165 170 175Ile Pro Ser Phe Leu Gly Asn Leu Thr Thr Leu Glu Lys Ile Asp Phe 180 185 190Ser Gln Asn Tyr Phe Glu Gly Glu Phe Pro Lys Glu Leu Val Arg Cys 195 200 205Thr Ser Leu Thr Tyr Leu Asp Leu Ser Phe Asn Arg Leu Thr Gly Gln 210 215 220Ile Pro Asp Asn Ile Ser Asn Leu Ile His Leu Glu Thr Leu Ile Leu225 230 235 240Ser Ser Asn Asn Leu Thr Gly Thr Ile Pro Lys Thr Leu Asp Arg Leu 245 250 255Val Asn Leu Thr His Phe Ala Ser Asn Lys Asn Gln Leu Ile Gly Arg 260 265 270Ile Pro Val Gln Leu Ala Lys Leu Thr Glu Leu His Phe Leu Asp Leu 275 280 285Ser Tyr Asn Gly Leu Asn Glu Thr Ile Pro Pro Glu Leu Phe Ala Leu 290 295 300Ser Asn Leu Gln Thr Leu Asp Leu Thr Lys Asn Leu Leu Thr Gly Glu305 310 315 320Ile Pro Gln Asn Phe Ser Arg Lys Leu Ile Arg Leu Arg Ile Gly Gln 325 330 335Asn Leu Leu Lys Gly Asn Ile Pro Leu Thr Ile Gly Asn Trp Ser Asn 340 345 350Leu Thr Tyr Leu Glu Met Asn Asp Asn Ser Leu Asp Gly Gln Ile Pro 355 360 365Gln Gln Leu Val Asn Cys Ile Lys Leu Gln Leu Leu Asp Leu Gly Asn 370 375 380Asn Asn Leu Ser Gly Ser Leu Thr Asn Gln Leu Pro Ser Leu Leu Gln385 390 395 400Leu Gln Val Leu Lys Leu His Asn Asn Asn Phe Val Gly Ser Ile Pro 405 410 415Tyr Ile Leu Ser Ser Phe Ser Asn Leu Ser Tyr Val Asp Leu Ser Asp 420 425 430Asn Thr Leu Asn Gly Ser Ile Pro Ser Asn Ile Phe Asn Leu Ser Lys 435 440 445Leu Gln Asn Leu Arg Leu Gln Asn Asn Lys Leu Thr Gly Ala Ile Pro 450 455 460Asn Thr Val Gly Gly Ser Gln Val Leu Leu Glu Leu Gln Leu Gly Gly465 470 475 480Asn Asn Leu Thr Gly Thr Met Pro Leu Glu Ile Gly Phe Val Arg Lys 485 490 495Leu Gln Ile Gln Leu Asn Leu Ser Cys Asn Ser Leu Glu Gly Glu Ile 500 505 510Pro Asn Thr Leu Ser Gly Leu Tyr Met Leu Glu Ile Leu Asp Leu Ser 515 520 525Asn Asn Lys Leu Thr Gly Glu Val Pro Gly Ser Leu Thr Ala Met Leu 530 535 540Ser Leu Thr Leu Leu Asn Ile Ser Asn Asn Ser Leu Thr Gly Val Leu545 550 555 560Pro Lys Phe Pro Asn Ser Thr Ser Ala Leu Ile Ile Ile Asp Thr Gly 565 570 575Asn Pro Gly Leu Thr Ala Gly Gln Asn Gly Ser Ala Pro Ala Ala Ser 580 585 590Ala Arg Lys Lys Ile Ser Ala Ile Leu Ile Ile Gly Val Ala Val Ala 595 600 605Gly Ala Val Phe Ala Ile Val Ala Val Gly Leu Phe Ile Val Ala Ser 610 615 620Lys Tyr Phe Gly Arg Gly Asp Gln Gln Met Pro Glu Val Gln Leu Ala625 630 635 640Arg Lys Ile Glu Gly His Phe Ile His Pro Asp Ser Ile His Arg Leu 645 650 655Arg Ile Asp Phe Glu Lys Gly Val Glu Ala Thr Leu Asp Pro Ala Asn 660 665 670Val Phe Leu Lys Asn Lys Phe Ser Thr Tyr Tyr Lys Ala Val Met Pro 675 680 685Ser Gly Ile Ser Tyr Ser Val Lys Lys Leu Asn Trp Ser Asp Arg Ile 690 695 700Phe Lys Ser Gly Ser Tyr Arg Lys Leu Gly Ala Glu Leu Glu Lys Gln705 710 715 720Gly Lys Leu Arg His Pro Asn Ile Leu Thr Pro Leu Ala His Val Leu 725 730 735Asp Thr Asp Ser Ala Tyr Leu Phe Tyr Glu Tyr Val His Lys Gly Ser 740 745 750Leu Ser Glu Phe Leu His Thr Ser Asn Val Ser Val Leu Asp Trp Pro 755 760 765Ser Arg Cys Arg Ile Ala Ile Gly Val Ala Gln Gly Leu Ala Phe Leu 770 775 780His Gly Cys Gln His Pro Ile Phe His Leu Asp Leu Thr Thr Lys Asn785 790 795 800Ile Leu Leu Lys Ser Leu Thr Glu Pro Gln Ile Gly Asp Ile Glu Leu 805 810 815Cys Lys Ile Val Asp Pro Ser Lys Ser Thr Gly Ser Ile Ser Ala Ile 820 825 830Ala Gly Ser Val Gly Tyr Val Pro Pro Glu Tyr Ala Tyr Thr Met Arg 835 840 845Val Thr Ala Ala Gly Asn Val Tyr Ser Phe Gly Val Ile Leu Leu Glu 850 855 860Leu Leu Thr Gly Arg Thr Pro Ile Thr Ser Gly Met Asp Leu Ala Lys865 870 875 880Trp Val Gln Ser Thr Leu Ser Gly Glu Glu Thr Trp Glu Gln Ile Leu 885 890 895Asp Thr Gly Ile Arg Asn Phe Ser Val Gln Ile Gln Asn Glu Met Ile 900 905 910Ala Met Leu Lys Val Ala Leu Ser Cys Val Ser Ser Ser Pro Glu Ser 915 920 925Arg Pro Lys Met Arg Asn Val Val Gly Met Leu Gln Met Val Arg Gln 930 935 940Val Ala Glu945 74 638 PRT Pinus radiata 74Met His Phe Lys Ile Leu Phe Asp Ala Lys Glu Glu Lys Ile Lys Ser 1 5 10 15Ser Phe Asp Met Leu Thr Ala Phe Leu Val Leu Cys Phe Thr Thr Thr 20 25 30Ala Ala Ser Ala Thr Gln Leu Ser Pro Ser Gly Leu Asn Tyr Glu Val 35 40 45Ala Ala Leu Met Ala Ile Lys Asn Ser Leu Asn Asp Pro His Asn Val 50 55 60Leu Glu Asn Trp Asp Ile Asn Ser Val Asp Pro Cys Gly Trp Arg Met65 70 75 80Val Thr Cys Thr Leu Glu Gly Ser Val Ser Ile Leu Gly Ile Gln Cys 85 90 95Gln Asn Leu Ser Gly Ser Leu Ser Pro Ser Ile Arg Asn Leu Thr Asn 100 105 110Leu Gln Ser Val Leu Leu Gln Asn Asn Ala Ile Ser Gly Ser Ile Pro 115 120 125Ala Glu Leu Gly Lys Leu Asp Lys Leu Asp Thr Leu Asp Leu Ser Asn 130 135 140Asn His Phe Asn Gly Leu Ile Pro Ser Ser Leu Gly Lys Leu Lys Asn145 150 155 160Leu Asn Tyr Leu Arg Leu Asn Asn Asn Asn Leu Ser Gly Pro Ile Pro 165 170 175Pro Ser Leu Ala Thr Ile Thr Gly Leu Thr Leu Leu Asp Leu Ser Cys 180 185 190Asn Asn Leu Ser Gly Ser Val Pro Arg Ile Ser Ala Arg Thr Phe Asn 195 200 205Ile Val Gly Asn Pro Leu Ile Cys Gly Pro Asn Ser Thr Tyr Lys Cys 210 215 220Pro Gly Gln Phe Pro Thr Pro Ile Pro Leu Val Val Glu Thr Pro Gln225 230 235 240Gly Arg Val Pro Ser Arg Gln Ser Lys Thr Arg Lys Leu Ala Val Ala 245 250 255Leu Val Ala Ser Leu Gly Phe Val Phe Val Val Ser Ile Gly Leu Leu 260 265 270Leu Trp Trp Arg Lys Arg His Asn Gln Gln Ile Phe Ile Asp Val Asn 275 280 285Glu Gln His Asn Val Asp Ile Cys Leu Gly His Leu Lys Arg Phe Ser 290 295 300Phe Lys Glu Leu Arg Val Ser Thr Asn Asn Phe Ser Ser Lys Asn Ile305 310 315 320Leu Gly Val Gly Gly Tyr Gly Ile Val Tyr Lys Gly Phe Leu Gln Asp 325 330 335Gly Thr Ile Val Ala Ile Lys Arg Leu Lys Asp Gly Asn Val Gly Gly 340 345 350Gly Glu Ile Gln Phe Gln Thr Glu Val Glu Met Ile Ser Leu Ala Val 355 360 365His Arg Asn Leu Leu Arg Leu Tyr Gly Phe Cys Thr Thr Ser Arg Glu 370 375 380Arg Leu Leu Val Tyr Pro Tyr Met Pro Asn Gly Ser Val Ala Ser Cys385 390 395 400Leu Arg Asp His Ile Asn Gly Lys Leu Ala Leu Asp Trp Pro Thr Arg 405 410 415Lys Arg Ile Ala Leu Gly Ala Ala Arg Gly Leu Leu Tyr Leu His Glu 420 425 430Gln Cys Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile 435 440 445Leu Leu Asp Glu Tyr Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala 450 455 460Lys Leu Leu Asp His Arg Asp Ser His Val Thr Thr Ala Val Arg Gly465 470 475 480Thr Val Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser 485 490 495Glu Lys Thr Asp Val Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Ile 500 505 510Thr Gly Gln Arg Ala Leu Asp Phe Gly Gln Ala Ala Lys Gln Lys Val 515 520 525Val Met Leu Asp Trp Val Lys Lys Leu His Gln Glu Lys Lys Leu His 530 535 540Leu Leu Ala Asp Lys Asp Leu Lys Gly Asn Phe Asp Arg Val Glu Leu545 550 555 560Glu Glu Met Val Gln Val Ser Leu Leu Cys Thr Gln Phe Gln Pro Gly 565 570 575His Arg Pro Lys Met Cys Asp Val Leu Arg Met Leu Glu Gly Asp Gly 580 585 590Leu Thr Glu Arg Trp Glu Thr Leu Gln Lys Ile Glu Thr Pro Arg Tyr 595 600 605Arg Val Thr Glu Ile Pro Ile Thr Tyr Ser Glu Leu Val Glu Glu Asp 610 615 620Ser Ser Trp Leu Val Gln Ala Ile Glu Leu Ser Gly Pro Arg625 630 635 75 659 PRT Eucalyptus grandis 75Met His Phe Arg Ile Leu Ala Leu Leu Gly Phe Phe Phe Leu Ala Val 1 5 10 15Leu Phe Pro Ser Ala Glu Pro Asp Leu Ala Ser Asp Arg Ala Ala Leu 20 25 30Leu Ala Leu Arg Ser Ala Val Gly Gly Arg Thr Leu Leu Trp Asn Ala 35 40 45Asn Leu Pro Ser Pro Cys Ser Trp Ala Gly Val Gln Cys Glu Gly Asn 50 55 60Arg Val Thr Ala Leu Arg Leu Pro Gly Val Ala Leu Ser Gly Gln Ile65 70 75 80Pro Asp Gly Val Leu Gly Asn Leu Thr Gln Leu Arg Thr Leu Ser Leu 85 90 95Arg Phe Asn Ala Leu Ser Gly Thr Leu Pro Ser Asp Leu Ala Ser Cys 100 105 110Ala Asp Leu Arg Asn Leu Tyr Val Gln Gly Asn Leu Phe Ser Gly Pro 115 120 125Ile Pro Ala Ser Leu Phe Gly Leu Ser Asp Leu Val Arg Leu Asn Leu 130 135 140Ala Ser Asn Lys Phe Ser Gly Asp Phe Pro Ala Gly Phe Gly Asn Leu145 150 155 160Thr Arg Leu Lys Thr Leu Leu Leu Glu Asn Asn Gln Leu Ser Gly Ser 165 170 175Ile Pro Ala Asp Leu Lys Gln Leu Lys Leu Glu Gln Phe Asn Val Ser 180 185 190Asn Asn Leu Leu Asn Gly Ser Ile Pro Glu Gly Leu Gly Ala Phe Ala 195 200 205Thr Ser Ser Phe Ser Gly Asn Ser Leu Cys Gly Lys Pro Leu Ala Ser 210 215 220Cys Ser Gln Asp Ile Ala Leu Pro Ala Gly Glu Pro Ser Gly Ser Pro225 230 235 240Gly Gln Pro Gly Gly Lys Lys Lys Lys Leu Ser Gly Ala Val Val Ala 245 250 255Gly Ile Val Ile Gly Cys Val Phe Gly Phe Ile Phe Leu Val Ile Leu 260 265 270Leu Ile Tyr Leu Cys Arg Lys Lys Gly Ser Lys Lys Ser Arg Ser Val 275 280 285Asp Val Ala Thr Phe Lys His Gln Glu Leu Glu Ile Pro Gly Glu Lys 290 295 300Pro Val Gly Glu Val Glu Asn Gly Gly Phe Ser Asn Gly Tyr Ser Val305 310 315 320Ala Ala Ala Ala Ala Ala Ala Met Thr Gly Ser Gly Lys Gly Glu Val 325 330 335Asn Gly Ser Ala Gly Ala Ala Ala Lys Lys Leu Ile Phe Phe Gly Asn 340 345 350Ser Ala Arg Ala Phe Asp Leu Glu Asp Leu Leu Arg Ala Ser Ala Glu 355 360 365Val Leu Gly Lys Gly Thr Phe Gly Thr Ala Tyr Lys Ala Val Leu Glu 370 375 380Ala Gly Ile Thr Val Ala Val Lys Arg Leu Lys Asp Val Asn Val Ala385 390 395 400Ala Lys Glu Phe Lys Glu Lys Ile Glu Ala Val Gly Ala Met Asp His 405 410 415Gln Ser Leu Val Pro Leu Arg Ala Tyr Tyr Tyr Ser Asn Asp Glu Lys 420 425 430Leu Leu Val Tyr Asp Tyr Met Pro Met Gly Ser Leu Ser Ala Leu Leu 435 440 445His Gly Asn Lys Gly Ala Gly Arg Thr Pro Leu Asn Trp Glu Ile Arg 450 455 460Ser Ala Ile Ala Leu Gly Ala Ala Arg Gly Ile Glu Tyr Leu His Ser465 470 475 480Gln Gly Pro Ile Val Ser His Gly Asn Ile Lys Ser Ser Asn Ile Leu 485 490 495Leu Thr Thr Ser Tyr Asp Ala Arg Val Ser Asp Phe Gly Leu Ala His 500 505 510Leu Val Gly Pro Ser Ser Thr Pro Asn His Val Ala Gly Tyr Arg Ala 515 520 525Pro Glu Val Thr Asp Pro Arg Lys Val Ser Gln Lys Ala Asp Val Tyr 530 535 540Ser Phe Gly Val Leu Leu Leu Glu Leu Leu Thr Gly Lys Ala Pro Ile545 550 555 560His Ser Gln Leu Asn Glu Glu Gly Val Asp Leu Pro Arg Trp Val Gln 565 570 575Ser Ile Val Arg Glu Glu Trp Thr Ser Glu Val Phe Asp Leu Glu Leu 580 585 590Leu Arg Tyr Gln Asn Ile Glu Glu Glu Met Val Gln Leu Leu Gln Leu 595 600 605Ala Ile Asp Cys Ala Ala Gln Tyr Pro Asp Lys Arg Pro Ser Met Ser 610 615 620Glu Val Arg Ser Gln Ile Glu Glu Leu Cys His Ser Ser Ser Gln Lys625 630 635 640Asp Arg Ala Pro Gln Leu Asp Gln Val Asn Glu Val Asn Asp Asp Thr 645 650 655Ser Ser Arg 76 968 PRT Eucalyptus grandis 76Met Glu Glu Ala Phe Leu Arg Leu Ile Phe Leu Val Ala Val Leu Leu 1 5 10 15Phe Gly Lys Asp Leu Gln Leu Val Phe Ser Phe Thr Asn Pro Asp Asp 20 25 30Ser Val Ala Leu Gln Ser Leu Lys Met Ser Trp Gln Asn Thr Pro Pro 35 40 45Ser Trp Glu Arg Ser Ser Asp Pro Cys Gly Leu Pro Trp Glu Gly Val 50 55 60Thr Cys Asn Ser Asn Ser Arg Val Thr Ser Leu Gly Leu Ser Thr Met65 70 75 80Gly Ile Lys Gly Gln Leu Ile Ser Glu Ile Ala Gly Leu Ala Glu Leu 85 90 95Arg Ser Leu Asp Leu Ser Phe Asn Lys Glu Leu Thr Gly Pro Leu Ala 100 105 110Arg Gln Leu Gly Asn Leu Gln Lys Leu Asn Ile Leu Ile Leu Ala Gly 115 120 125Cys Ser Phe Thr Gly Ser Ile Pro Asp Glu Leu Gly Asn Leu Ala Glu 130 135 140Leu Ser Phe Leu Ala Leu Asn Ser Asn Asn Leu Thr Gly Asn Ile Pro145 150 155 160Ala Ser Leu Gly Asn Leu Ser Lys Leu Tyr Trp Phe Asp Leu Ala Asp 165 170 175Asn Gln Leu Thr Gly Pro Ile Pro Ile Ser Thr Asp Thr Ser Pro Gly 180 185 190Leu Asp Leu Leu Leu Lys Ala Lys His Phe His Phe Asn Lys Asn Lys 195 200 205Leu Ser Gly Pro Ile Pro Glu Lys Leu Phe Asn Ser Ala Met Val Leu 210 215 220Ile His Val Leu Phe Asp Gly Asn Gln Leu Asn Gly Ser Ile Pro Ser225 230 235 240Ser Val Gly Leu Leu Pro Asp Leu Glu Val Leu Arg Leu Asp Arg Asn 245 250 255Lys Leu Ser Gly Lys Val Pro Leu Asn Leu Asn Asn Leu Thr Asn Leu 260 265 270Ser Glu Leu Asn Phe Ala His Asn Ala Leu Thr Gly Pro Leu Pro Asp 275 280 285Leu Thr Asp Met Asn Ser Leu Asn Tyr Val Asp Leu Ser Asn Asn Phe 290 295 300Phe Asp Pro Ser Glu Ala Pro Asp Trp Phe Ser Thr Leu Pro Thr Leu305 310 315 320Thr Thr Leu Val Ile Glu Tyr Gly Pro Leu Lys Gly Val Val Pro Gln 325 330 335Lys Leu Phe Ser Phe Pro Gln Leu Gln Gln Val Lys Leu Lys Asn Asn 340 345 350Glu Phe Asn Gly Thr Leu Asn Met Gly Asp Asn Ile Ser Pro Gln Leu 355 360 365Gln Leu Val Asp Leu Gln Asn Asn Gln Ile Ser Ser Val Thr Leu Gly 370 375 380Ser Ser Gly Tyr Ser Asn Thr Leu Met Leu Ile Gly Asn Pro Val Cys385 390 395 400Thr Thr Glu Leu Ser Asn Thr Asn Tyr Cys Gln Leu Gln Gln Gln Thr 405 410 415Val Lys Pro Tyr Ser Thr Ser Leu Ala Ser Cys Gly Ser Lys Ser Cys 420 425 430Pro Pro Asp Glu Arg Leu Asn Pro Gln Ser Cys Glu Cys Ala Phe Pro 435 440 445Tyr Glu Gly Thr Leu Tyr Phe Arg Gly Pro Ser Phe Arg Glu Leu Ser 450 455 460Asn Val Thr Leu Phe His Met Leu Glu Met Asp Leu Trp Thr Lys Leu465 470 475 480Asn Leu Thr Pro Gly Ser Val Ser Leu Gln Asn Pro Phe Phe Asn Leu 485 490 495Asp Asp Tyr Leu Gln Val Gln Leu Ser Leu Phe Pro Pro Ser Gly Lys 500 505 510Tyr Phe Ser Arg Ser Asp Ile Gln Ser Ile Gly Phe Asp Leu Thr Asn 515 520 525Gln Thr Phe Lys Pro Pro Lys Pro Phe Gly Pro Tyr Tyr Phe Ile Ala 530 535 540Ser Pro Tyr Ala Phe Pro Asp Asn Gly Gly Thr Ala Ile Ser Lys Gly545 550 555 560Val Ile Val Gly Ile Ala Ile Gly Gly Thr Val Leu Val Leu Gly Leu 565 570 575Val Val Leu Gly Leu Tyr Ala Ile Arg Gln Lys Lys Arg Ala Glu Lys 580 585 590Ala Leu Glu Leu Ser Arg Pro Phe Ala Ser Trp Ala Pro Ser Gly Lys 595 600 605Asp Ser Gly Gly Ala Pro Gln Leu Lys Gly Ala Arg Trp Phe Ser Tyr 610 615 620Asp Glu Leu Lys Arg Cys Thr Asn Asn Phe Ser Asp Ser Asn Glu Leu625 630 635 640Gly Phe Gly Gly Tyr Gly Lys Val Tyr Arg Gly Val Leu Pro Asp Gly 645 650 655His Ile Leu Ala Ile Lys Arg Ala Gln Gln Gly Ser Met Gln Gly Ala 660 665 670Thr Glu Phe Lys Thr Glu Ile Glu Leu Leu Ser Arg Val His His Lys 675 680 685Asn Leu Val Gly Leu Ile Gly Phe Cys Phe Glu Gln Gly Glu Gln Met 690 695 700Leu Val Tyr Glu Tyr Met Pro Asn Gly Thr Leu Arg Asp Ser Leu Thr705 710 715 720Gly Lys Ser Gly Ile Tyr Leu Asp Trp Lys Arg Arg Leu Arg Ile Ala 725 730 735Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Glu Leu Ala Asn Pro 740 745 750Pro Ile Ile His Arg Asp Val Lys Ser Thr Asn Ile Leu Leu Asp Glu 755 760 765His Leu Thr Ala Lys Val Ala Asp Phe Gly Leu Ser Lys Leu Val Ser 770 775 780Asp Ser Gly Lys Gly His Val Ser Thr Gln Val Lys Gly Thr Leu Gly785 790 795 800Tyr Leu Asp Pro Glu Tyr Tyr Met Ser Gln Gln Leu Thr Glu Lys Ser 805 810 815Asp Val Tyr Ser Phe Gly Val Val Met Leu Glu Leu Ile Thr Ala Lys 820 825 830Gln Pro Ile Glu Lys Gly Lys Tyr Val Val Arg Glu Ile Arg Thr Ala 835 840 845Met Asp Lys Asn Asp Gln Asp Tyr Tyr Gly Val Arg Glu Met Met Asp 850 855 860Pro Ser Met Arg Ser Met Gly Tyr Leu Val Gly Phe Ser Arg Phe Leu865 870 875 880Asp Leu Ala Met Arg Cys Val Glu Glu Ser Ala Ala Asp Arg Pro Thr 885 890 895Met Ser Glu Val Val Lys Ala Ile Glu Thr Met Leu Gln Asn Asp Gly 900 905 910Ile His Thr Asn Ser Thr Ser Ala Ser Ser Ser Ala Thr Asp Phe Gly 915 920 925Ser Thr Lys Gly Ala Pro Arg His Pro Tyr Asn Asp Ala Leu Pro Lys 930 935 940Lys Glu Val Ser Tyr Ser Asp Ser Phe Asp Tyr Ser Gly Gly Tyr Gly945 950 955 960Leu Ser Thr Lys Ile Glu Pro Lys 965 77 632 PRT Pinus radiata 77Met Leu Phe Pro Trp Arg Ser Leu Val Leu Ile Ala Phe Thr Ser Leu 1 5 10 15Val Val Gln Leu Ile Pro Ala Gln Ala Val Glu Asp Arg Arg His Asp 20 25 30Thr Thr Phe Leu Phe Asp Gly Phe Asn Gly Thr Asn Leu Ile Leu Glu 35 40 45Ala Asn Ala Ser Val Ile Gly Ser Glu Ser Val Leu Ser Leu Thr Asn 50 55 60His Ser His Glu Phe Met Leu Gly Arg Ala Leu Tyr Ala Ala Pro Val65 70 75 80Gln Met Lys Asn Asn His Thr Val Ser Ser Phe Ser Thr Thr Phe Val 85 90 95Phe Ser Ile Val Pro Pro Pro Ser Asn Glu Gly Gly His Gly Leu Ala 100 105 110Phe Ile Met Thr Pro Tyr Thr Ser Pro Met Gly Ala Gln Pro Val Gln 115 120 125Tyr Leu Gly Leu Leu Asn Leu Thr Ser Asn Gly Gln Pro Tyr Asn His 130 135 140Leu Phe Ala Val Glu Phe Asp Thr Ile Met Asn Val Glu Phe Lys Asp145 150 155 160Pro Asp Arg Asn His Val Gly Val Asp Ile Asn Ser Leu Ile Ser Val 165 170 175Gln Thr Glu Thr Ala Gly Tyr Trp Asn Gly Glu Glu Phe His Glu Leu 180 185 190Asn Leu Arg Ser Gly Arg Asn Ile Gln Ala Trp Ile Asp Tyr Asp His 195 200 205Leu Glu Ser Ser Leu Asn Val Thr Ile Thr Val Ala Gly Leu Pro Arg 210 215 220Pro Gln Arg Pro Leu Ile Ser Leu Gln Ile Asp Leu Gln Asn Ile Val225 230 235 240Glu Glu Lys Met Leu Val Gly Phe Ser Ala Ala Thr Gly Leu Leu Val 245 250 255Glu Asp His Tyr Ile Leu Ala Trp Ser Phe Thr Thr Glu Asp Thr Ala 260 265 270Pro Pro Leu Asp Val Ser Cys Leu Ser Ser Phe Ala Asn Met Tyr Ser 275 280 285Glu Pro Leu Ser Arg Gly Phe Ile Ala Gly Val Thr Val Val Ser Val 290 295 300Val Leu Phe Trp Leu Val Ile Ala Ala Ala Met Phe Leu Arg Arg Thr305 310 315 320Leu Asn Arg Glu Thr Val Glu Glu Trp Glu Gln Glu Tyr Trp Pro His 325 330 335Arg Phe Asp Tyr Lys Glu Leu Arg Ile Ala Thr Arg Gly Phe Arg Asp 340 345 350Glu Asn Leu Leu Gly Tyr Gly Gly Phe Gly Met Val Tyr Lys Gly Phe 355 360 365Leu Pro Arg Ser Gly Gln Glu Val Ala Val Lys Cys Ile Thr Thr Glu 370 375 380Phe Lys Glu Gly Ile Lys Gly Phe Val Ala Glu Ile Ser Ser Met Gly385 390 395 400Arg Leu Gln His Arg Asn Leu Val Gln Leu Arg Gly Trp Cys Arg Arg 405 410 415His Thr Gln Leu Phe Ile Val Tyr Asp Tyr Met Pro Asn Gly Ser Leu 420 425 430His Lys Leu Ile Phe Gly Ser Pro Thr Thr Val Leu Pro Trp His Arg 435 440 445Arg Tyr Ala Ile Leu Lys Gly Val Ala Ala Gly Leu Leu Tyr Leu His 450 455 460Glu Gln Trp Glu Lys Arg Val Val His Arg Asp Ile Lys Ser Ser Asn465 470 475 480Val Leu Leu Asp Ser Glu Phe Asn Gly Arg Leu Ser Asp Phe Gly Leu 485 490 495Ala Arg Leu Tyr Asp His Ser Glu Asn Pro Glu Thr Thr Tyr Val Val 500 505 510Gly Thr Leu Gly Tyr Ile Ala Pro Glu Leu Ile Gln Thr Gly Lys Ala 515 520 525Thr Pro Ser Ser Asp Val Phe Ser Phe Gly Val Leu Leu Leu Glu Val 530 535 540Ala Cys Gly Lys Ser Pro Val Asp Ser Leu Glu Asp Ser Glu Arg Met545 550 555 560Ile Leu Val Glu Trp Ala Trp Glu Leu Tyr Thr Glu Gly Arg Leu Leu 565 570 575Glu Ala Ser Asp Pro Lys Leu Ala Ala Lys Gly Gly Tyr Asp Glu Gly 580 585 590Glu Met Glu Lys Val Leu Lys Leu Gly Leu Leu Cys Ser His Pro Glu 595 600 605Pro Glu Ser Arg Leu Ser Met Arg Gln Val Cys Gln Val Leu Asn Gly 610 615 620Glu Ala Pro Val Pro Cys Arg Trp625 630 78 903 PRT Eucalyptus grandis 78Met Gly Leu Lys Ile Phe Ser Val Gly Phe Ala Leu Leu Cys Cys Phe 1 5 10 15Cys Ser Leu Gly Phe Cys Asp Gln Asp Gly Phe Leu Ser Leu Ala Cys 20 25 30Gly Gly Thr Thr Asn Tyr Thr Asp Ser Ser Asn Ile Trp Trp Ile Thr 35 40 45Asp Ser Asp Phe Ile Ser Thr Gly Lys Thr Thr Tyr Val Asp Asn Ile 50 55 60Glu Gly Asn Ser Ser Gly Val Ser Leu Arg Phe Phe Pro Asp Ser Lys65 70 75 80Val His Asn Cys Tyr Arg Leu Pro Val Arg Asn Ile Ser Ser Leu Ile 85 90 95Leu Val Arg Ser Gln Phe Val Tyr Lys Asn Tyr Asp Gly Arg Gly Lys 100 105 110Pro Pro Ala Phe Ser Val Ser Leu Gly Thr Ala Met Ala Ser Thr Ile 115 120 125Asn Leu Thr Thr Asn Asp Pro Trp Thr Glu Glu Phe Ile Trp Pro Val 130 135 140Asp Lys Asp Thr Leu Ser Phe Cys Leu His Arg Ile Arg Asn Gly Gly145 150 155 160Thr Pro Val Ile Ser Leu Leu Glu Val Arg Pro Leu Pro Pro Glu Ala 165 170 175Tyr Lys Ser Gly Met Gly Asp Tyr Pro Asn Lys Leu Leu Arg Lys Ser 180 185 190Tyr Arg Ile Asp Ser Gly Tyr Thr Asn Gly Ser Leu Lys Tyr Pro Ala 195 200 205Asp Pro Tyr Asp Arg Ile Trp Asp Ala Asp Lys Ser Phe Thr Pro Phe 210 215 220His Val Thr Thr Gly Phe Lys Ile Gln Val Glu Phe Asn Leu Ser Gly225 230 235 240Leu Ser Glu Ser Pro Pro Pro Ala Val Leu Gln Thr Ala Arg Val Leu 245 250 255Ala Arg Lys Glu Val Leu Thr Tyr Asn Phe Pro Leu Asp Ser Leu Gly 260 265 270Asp Tyr Tyr Val Val Leu Tyr Phe Ala Gly Ile Leu Pro Ile Ser Pro 275 280 285Ser Phe Asp Val Ile Ile Asn Gly Asp Ile Val Gln Ser Asn Tyr Thr 290 295 300Val Lys Thr Ser Ala Ala Ser Ala Leu Tyr Val Thr Arg Lys Lys Ile305 310 315 320Lys Ser Leu Asn Val Thr Leu Lys Ser Lys Arg Phe Phe Pro Gln Val 325 330 335Asn Ala Ile Glu Val Tyr Glu Leu Val Asp Ile Pro Pro Glu Ala Ser 340 345 350Ser Thr Thr Val Ser Ala Leu Gln Val Ile Glu Gln Phe Thr Gly Leu 355 360 365Asp Leu Gly Trp Glu Asp Asp Pro Cys Ser Pro Lys Pro Trp Asp His 370 375 380Val Gly Cys Glu Gly Ser Leu Val Thr Ser Leu Asp Leu Ser Asp Ile385 390 395 400Asn Leu Arg Ser Ile Ser Pro Thr Phe Gly Asp Leu Leu Asp Leu Lys 405 410 415Thr Leu Asp Leu His Asn Ala Ser Leu Ala Gly Glu Ile Gln Asn Leu 420 425 430Asp Ser Leu Gln Asn Leu Glu Lys Leu Asn Leu Ser Phe Asn Lys Leu 435 440 445Thr Ser Phe Gly Ser Asp Trp Glu Asn Leu Ile Ser Leu Gln Val Leu 450 455 460Asp Val Gln Asn Asn Ser Leu Asp Gly Val Val Pro Asp Gly Leu Gly465 470 475 480Glu Leu Lys Asp Leu His Leu Leu Asp Leu Glu Asn Asn Leu Leu Gln 485 490 495Gly Thr Leu Pro Asp Ser Leu Asn Arg Gln Ser Leu Glu Val Arg Thr 500 505 510Ser Gly Asn Leu Cys Leu Ser Phe Ser Thr Thr Ala Cys Gly Asp Ala 515 520 525Ser Ser Ser Pro Ser Ile Glu Ala Pro Gln Val Thr Ile Val Pro Glu 530 535 540Arg Asn Lys Gly Gly His Asn Arg Leu Ala Ile Ile Leu Gly Ala Val545 550 555 560Gly Gly Val Ser Leu Ala Ile Leu Leu Ile Pro Leu Phe Val Phe Met 565 570 575Tyr Arg Arg Arg Gly Arg Thr Glu Met Ser Tyr Thr Glu Arg Ala Val 580 585 590Ala Asp Val Arg Asn Trp Asn Ala Ala Lys Ile Phe Ser Tyr Lys Glu 595 600 605Ile Lys Thr Ala Thr Asn Asn Phe Lys Glu Val Ile Gly His Gly Ser 610 615 620Phe Gly Ser Val Tyr Leu Gly Asn Leu Pro Val Gly Lys Leu Val Ala625 630 635 640Val Lys Val Arg Phe Asp Lys Thr Gln Leu Gly Ala Asp Ser Phe Ile 645 650 655Asn Glu Val Arg Leu Leu Ser Gln Val Arg His Gln Asn Leu Val Ser 660 665 670Leu Glu Gly Phe Cys Tyr Glu Ser Gln Arg Gln Ile Leu Val Tyr Glu 675 680 685Tyr Leu Pro Gly Gly Ser Leu Ala Asp Gln Leu Tyr Gly Pro Asn Ser 690 695 700Arg Lys Phe Ser Leu Ser Trp Val Arg Arg Leu Lys Ile Ala Val Asp705 710 715 720Ala Ala Lys Gly Leu Asp Tyr Leu His Asn Gly Ser Asn Pro Arg Ile 725 730 735Ile His Arg Asp Ile Lys Cys Ser Asn Ile Leu Leu Asp Lys Glu Met 740 745 750Asn Ala Arg Leu Cys Asp Phe Gly Leu Ser Lys Gln Met Ile Gln Pro 755 760 765Asp Ala Thr His Val Thr Thr Val Val Lys Gly Thr Ala Gly Tyr Leu 770 775 780Asp Pro Glu Tyr Tyr Ser Thr Gln Gln Leu Thr Glu Lys Ser Asp Val785 790 795 800Tyr Ser Phe Gly Val Val Leu Leu Glu Leu Ile Cys Gly Arg Glu Pro 805 810 815Leu Asn His Ser Gly Thr Pro Asp Ser Phe Asn Leu Val Leu Trp Ala 820 825 830Lys Pro Tyr Leu Gln Ala Gly Ala Phe Glu Ile Val Asp Glu Ser Leu 835 840 845Gly Gly Ser Phe Asp Val Glu Ser Met Arg Lys Val Ala Lys Ile Ala 850 855 860Val Arg Ser Val Glu Arg Asp Ala Ser Leu Arg Pro Thr Ile Ala Gln865 870 875 880Ile Leu Ser Val Leu Lys Glu Ala Tyr Ser Ile Gln Leu Ser Tyr Leu 885 890 895Ala Ala Ser Gly His Val Asn 900 79 711 PRT Pinus radiata 79Met Gly Cys Arg Lys Ala Val Arg Ser Val Ser Pro Gln Asn Pro Ser 1 5 10 15Ser Ser Leu Met His Leu Arg Leu Leu Gly Val Leu Leu Met Leu His 20 25 30Cys Ile Asp Gly Leu Ile Leu Asn Pro Glu Val Ser Thr Leu Leu Thr 35 40 45Leu Lys Pro Thr Leu Ser Gly Thr Gly Val Asn Val Val Leu Ala Ser 50 55 60Trp Ser Ala Ser Arg Pro Leu Cys Gly Trp Lys Gly Val Met Trp Met65 70 75 80Tyr Asn Gly Ile Pro Val Asn Cys Ser Val Pro Lys Phe Arg His Ser 85 90 95Val Ala Leu Ala Tyr Ser Arg Lys Val Ser Val Leu Gly Ile Asp Leu 100 105 110Glu Ala Thr Gly Leu Lys Gly Thr Val Pro Lys Glu Leu Gly Ser Phe 115 120 125Ile Tyr Leu Gln Gln Leu Asn Leu Asn Asn Asn Ser Leu Thr Gly Gly 130 135 140Met Pro Pro Glu Leu Gly Asn Val Pro Asn Leu Ser Ser Leu Gln Leu145 150 155 160Lys Asn Asn Gly Leu Asn Gly Gly Ile Pro Thr Ala Ile Trp Asn Leu 165 170 175Cys Asp Asn Ile Thr Glu Leu Glu Leu Gly Phe Asn Glu Leu Ser Gly 180 185 190Ser Ile Pro Glu Pro Gly Asn Gly Ser Asn Ile Gly Cys Pro Gln Leu 195 200 205Arg Arg Phe Glu Val Asn Asn Asn Ser Leu Thr Gly Thr Ile Pro Ser 210 215 220Phe Leu Ala Asn Cys Ile Ser Leu Gln Glu Leu Asp Leu Ser Gly Asn225 230 235 240Ser Phe Thr Gly Glu Ile Pro Asn Glu Leu Ala Asn Leu Pro Asn Leu 245 250 255Thr Thr Leu Asn Leu Ala His Asn Asn Leu Ser Gly Arg Ile Pro Ser 260 265 270Phe Arg Gln Lys Phe Asp Lys Asn Ser Phe Val Glu Asn Ser Gly Ser 275 280 285Leu Cys Gly Gln Pro Leu Leu Asn Pro Cys Gly Val Ala Pro Asn Ala 290 295 300Ala Ser Ala Ala Ser Ala Asn Val Thr Ala Ala Gln Phe Asn Val Thr305 310 315 320Lys Ala His Phe Lys Ser Met Ser Thr Gly Ala Ile Ala Gly Ile Ile 325 330 335Ile Gly Ser Ile Ala Val Val Val Ile Ala Ser Ser Leu Leu Ile Gly 340 345 350Cys Tyr His Arg Phe Ser Thr Asp Ala Thr Asp Lys Ser Ser Ser Ser 355 360 365Ser Ala Pro Ser Lys Lys Asp Lys Glu Glu Asp Met Asp Asn Val Ser 370 375 380Gly Lys Leu Ile Asn Phe Gln Gly Gly Glu His Leu Thr Val Asp Asp385 390 395 400Val Leu Asn Ala Thr Gly Glu Val Leu Gly Lys Ser Ser Tyr Gly Thr 405 410 415Val Tyr Lys Ala Arg Leu Ser Ser Gly Cys Met Ile Ala Leu Arg Leu 420 425 430Leu Arg Asp Gly Cys Leu Arg Ser Thr Asp Glu Phe Met Pro Ala Ile 435 440 445Gln Glu Leu Gly Thr Ile Arg His Lys His Leu Val Ser Leu Arg Ala 450 455 460Phe Tyr Ser Gly Thr Arg Gly Glu Lys Leu Leu Ala Tyr Asp Tyr Leu465 470 475 480Pro Arg Gly Ser Leu Ala Glu Leu Leu His Ser Thr Asn Arg Pro Ala 485 490 495Pro Gly Trp Ala Arg Arg His Lys Ile Ala Leu Gly Ala Ala Arg Gly 500 505 510Leu Ala Tyr Leu His Thr Gly Phe His Lys Ser Ile Ile His Gly Asn 515 520 525Ile Lys Ser Lys Asn Ile Leu Val Asp Asp Asn Tyr Val Ala His Leu 530 535 540Ser Asp Tyr Gly Leu Asn Lys Leu Met Asn Ser Thr Ala Asn Ile Glu545 550 555 560Met Leu Glu Ala Ala Ala Ser Gln Gly Tyr Lys Ala Pro Glu Leu Ile 565 570 575Lys Met Lys Arg Ala Asn Ala Lys Thr Asp Ile Tyr Ser Phe Gly Ile 580 585 590Gly Leu Leu Glu Ile Leu Thr Gly Arg Arg Pro Gly Arg Thr Ser Ser 595 600 605Ser Asn His Ile Val Asp Leu Pro Thr Val Val Lys Asn Ala Val Leu 610 615 620Glu Glu Arg Ile Ser Glu Leu Phe Asp Leu Glu Leu Leu Arg Ala Met625 630 635 640Arg Ser Pro Ala Asp Glu Gly Leu Leu Gln Val Leu Gln Leu Ala Met 645 650 655Gly Cys Cys Ala Pro Ser Pro Ser Val Arg Pro His Ile Lys Glu Val 660 665 670Val Arg Gln Leu Glu Glu Ile Arg Pro Lys Pro Gln Ser Pro His Leu 675 680 685Ala Leu Ser Pro Gln Tyr Asn Ser Asp Asp Lys Ser Ser Arg Glu Phe 690 695 700Leu Leu Asp Gly Gly Gln Asn705 710 80 707 PRT Pinus radiata 80Met Gly Lys Lys Ile Leu Gln Cys Leu Arg Leu Ile Ile Ala Ile Leu 1 5 10 15Thr Ala Ser Ile Ala Ile Ser His Gly Thr Thr Asp Pro Asp Asp Val 20 25 30Ser Ala Leu Lys Gly Ile Tyr Ser Ser Leu Asn Ser Pro Gln Gln Leu 35 40 45Ser Gly Trp Ser Ala Asn Gly Gly Asp Pro Cys Gly Gln Ser Trp Lys 50 55 60Gly Val Ser Cys Ser Gly Ser Ser Val Thr Leu Ile Lys Leu Ser Gly65 70 75 80Leu Gly Leu Ser Gly Ser Leu Tyr Tyr Gln Leu Ser Asp Leu Ser Ser 85 90 95Leu Thr Thr Leu Asp Leu Ser Asn Asn Asn Ile Gln Gly Asn Ile Pro 100 105 110Tyr Ala Leu Pro Gln Lys Leu Gln Glu Leu Asn Leu Ala Ser Asn Gly 115 120 125Leu Ser Gly Thr Ile Pro Tyr Ser Ile Ser Asn Met Thr Gly Leu Thr 130 135 140Asp Leu Lys Leu Ser His Asn Gln Leu Ser Gly Gln Ile Gln Asp Ile145 150 155 160Phe Gly Gln Leu Ser Ser Leu Ser Thr Leu Asp Leu Ser Phe Asn Thr 165 170 175Leu Thr Gln Asn Leu Pro Gln Ser Phe Ser Ser Leu Ser Ser Leu Ser 180 185 190Val Leu Tyr Leu Gln Asn Asn Gln Leu Ala Gly Ser Val Asn Val Leu 195 200 205Ala Asn Leu Pro Leu Thr Asp Leu Asn Ile Glu Asn Asn Arg Phe Ser 210 215 220Gly Trp Val Pro Asn Ala Trp Arg Ser Asn Gln Asn Phe Lys Tyr Ser225 230 235 240Ser Gly Asn Ser Phe Ala Thr Gly Pro Ala Pro Pro Pro Pro Pro Tyr 245 250 255Thr Pro Pro Pro Pro Ser Asn Asn Arg Pro Pro Lys Ser Ser Asn Val 260 265 270Val Pro Ser Ser Gly Gly Ser Lys Gly Gly Asn Ser Asn Lys Lys Ser 275 280 285Leu Ser Gly Gly Ala Ile Val Gly Ile Ile Phe Ala Val Ile Leu Thr 290 295 300Val Val Ala Ala Ile Leu Gly Val Ile Leu Tyr Ala Arg Lys Ser Pro305 310 315 320Arg Arg Glu Gln Asp Glu Glu Lys Leu Ser Asn Arg Val Ser Phe Thr 325 330 335Pro Leu Ser Pro Leu Asp Ala Glu Leu Leu Lys Glu Ser Pro Glu Gln 340 345 350Lys Val Ser Ser Ser Pro Leu Glu Ile Ala Leu Lys Pro Pro Pro Ser 355 360 365Glu Arg Asn Lys Ser Thr Gly Asp Lys Gly Phe Gly Ser Ile Phe Ser 370 375 380Ser Lys Arg Thr Lys Asn Pro Ile Ser Ala Thr Glu Tyr Ser Ile Ala385 390 395 400Asp Leu Gln Met Ala Thr Asn Ser Phe Ser Gln Asp Asn Leu Ile Ala 405 410 415Glu Gly Ala Leu Gly Arg Ile Tyr Arg Ala Glu Phe Pro Asp Gly Lys 420 425 430Ile Leu Ala Val Lys Lys Leu Asp Thr Ser Thr Leu Ser Leu Gln Arg 435 440 445Pro Glu Asp Phe Leu Asp Ala Val Ser Asn Ile Ser Arg Leu His His 450 455 460Pro Asn Ile Thr Glu Leu Val Gly Tyr Cys Thr Glu His Glu Gln Tyr465 470 475 480Leu Leu Val Tyr Glu Tyr Phe Asp Asn Gly Ser Leu Tyr Asp Val Leu 485 490 495His Met Ala Asp Glu Thr Thr Arg Asn Leu Ser Trp Asn Ile Arg Val 500 505 510Lys Ile Ala Leu Gly Ser Ala Arg Val Leu Glu Tyr Leu His Glu Val 515 520 525Cys Ser Pro Ser Ile Val His Lys Lys Phe Lys Ser Ser Asn Ile Leu 530 535 540Leu Asp Asp Asp Phe Asn Pro Arg Leu Ser Asp Cys Gly Ile Ala Ala545 550 555 560Leu Asn Pro Asn Ser Glu Arg Gln Val Gln Val Leu Gly Ser Phe Gly 565 570 575Tyr Ser Ala Pro Glu Tyr Val Met Ser Gly Ile Tyr Thr Met Lys Ser 580 585 590Asp Val Tyr Ser Phe Gly Val Val Met Leu Glu Leu Leu Thr Gly Arg 595 600 605Lys Pro Leu Asp Ser Ser Arg Thr Arg Ser Glu Gln Ser Leu Val Arg 610 615 620Trp Ala Thr Pro Gln Leu His Asp Ile Asp Ala Leu Ala Lys Met Val625 630 635 640Asp Pro Ala Leu Lys Gly Ser Tyr Pro Ala Lys Ser Leu Ser Arg Phe 645 650 655Ala Asp Ile Ile Ala Leu Cys Ile Gln Pro Glu Pro Glu Phe Arg Pro 660 665 670Pro Met Ser Glu Val Val Gln Ala Leu Val Arg Met Met Gln Arg Ala 675 680 685Ser Leu Asn Lys Arg Met Thr Gly Asp Glu Thr Ala Asp His Asp Pro 690 695 700Ala Asp Tyr705 81 20 PRT Pinus radiata 81Met Gln Gln Pro Tyr Val Val Leu Ala Leu Trp Trp Ile Leu Val Leu 1 5 10 15Arg His Pro Leu 20 82 89 PRT Eucalyptus grandis 82Met Arg Val Leu Phe Ile Val Leu Gly Val Val Leu Leu Cys Thr Val 1 5 10 15Ile Pro Gly Ser Ser Ser Ser Val Ser Asp Val Asp Val Leu Leu Ala 20 25 30Leu Lys Gln Gly Phe Gln Ser Pro Glu Pro Ala Leu Ile Thr Trp Ser 35 40 45Ser Ser Asn Ser Ser Ser Val Cys Leu Trp Tyr Gly Ile Arg Cys Ser 50 55 60Arg Gly Arg Val Val Ser Leu Gln Leu Thr Asp Leu Asn Leu Gly Gly65 70 75 80Ser Val Ser Pro Pro Val Ser Arg Leu 85 83 153 PRT Pinus radiata 83Met Leu Leu Cys Phe Val Leu Val Ala Gly Thr Ser Ala Ala Val Asp 1 5 10 15Asp His Gln Tyr Leu Tyr His Pro Ser His Tyr Val Met Asn Glu Arg 20 25 30Gln Glu Ser Gly Ser Ser Met Ala Arg His Glu Lys Arg Asp Val Glu 35 40 45Ala Leu Leu Ser Phe Arg Asn Ala Ile Thr Ala Asp Pro His Gly Leu 50 55 60Leu Ser Asn Trp Thr Ala His Asn Ser Ala Asn Ile Cys Ser Trp Asn65 70 75 80Gly Ile Gly Cys Arg Lys Gln Ser Arg Arg Val Val Ser Ile Tyr Leu 85 90 95Arg Phe Ser His Leu Glu Gly Thr Leu Ser Pro Ser Val Gly Asn Ile 100 105 110Ser Leu Leu His Thr Phe Val Leu Thr Val Asn Lys Leu Thr Gly Arg 115 120 125Ile Pro Pro Glu Phe Gly Gln Leu Lys Ala Leu Gln Thr Leu Asp Leu 130 135 140Tyr Arg Asn Leu Leu Ser Ser Ser Gly145 150 84 154 PRT Pinus radiata 84Met Asp Leu Leu Leu Leu Leu Leu Val Met Met Gly Val Ala Met Pro 1 5 10 15Thr His Ser Gln His Thr Gly Gly Phe Thr Ser Val Gln Arg Phe Pro 20 25 30Phe Asn Gly Arg Ser Met Met Gly Lys Pro Ser Ile Ala Gly Tyr His 35 40 45Glu Lys Arg Asp Val Glu Ala Leu Leu Ser Phe Arg Lys Gly Ile Thr 50 55 60Leu Asp Pro Tyr Gly Trp Leu Ser Asn Trp Thr Ala Asn Asn Ser His65 70 75 80Asn Val Cys Leu Trp Asn Gly Ile Ser Cys Ser Pro Asn Thr Asn Arg 85 90 95Val Val Glu Ile Ser Leu Arg Tyr Gly Arg Leu Asn Gly Thr Leu Ser 100 105 110Pro Tyr Ile Gly Asn Leu Ser Leu Leu Arg His Leu Asp Leu Ser Ser 115 120 125Asn Ala Leu Ser Gly Arg Ile Pro Ala Lys Phe Gly Gln Leu Lys Ala 130 135 140Leu Arg Ile Leu Asp Leu Ser Asn Asn Ala145 150 85 82 PRT Eucalyptus grandis 85Met Val Phe Arg Arg Phe Val Val Met Leu Phe Ile Cys Thr Ala Ser 1 5 10 15Val Cys Ala Gly Leu Thr Asp Pro Arg Asp Val Ala Ala Ile Asn Ser 20 25 30Leu Tyr Val Ser Leu Gly Tyr Pro Pro Leu Arg Gly Trp Leu Leu Val 35 40 45Gly Gly Asp Pro Cys Val Asp Asn Trp Glu Gly Val Glu Cys Val Ile 50 55 60Ser Asn Ile Thr Gly Leu Asn Leu Ser Gly Ala Asn Leu Gly Gly Glu65 70 75 80Leu Gly 86 103 PRT Eucalyptus grandis 86Met Gly Phe Phe Tyr Gln Val Phe Leu Ile Phe Leu Ala Val Ala Pro 1 5 10 15Ser Ala Leu Cys Gln Val Thr Glu Phe Val Ser Ile Asp Cys Gly Gly 20 25 30Ser Ser Asn Tyr Thr Asp Pro Thr Thr Gly Leu Ala Trp Ile Pro Asp 35 40 45Thr Gly Leu Met Ser Tyr Gly Gln Ser Ser Lys Val Gln Asn Pro Asn 50 55 60Val Ser Ser Val Gln Tyr Ser Thr Arg Arg Asp Phe Pro Ile Asp Gly65 70 75 80Gln Lys Tyr Cys Tyr Thr Leu Arg Thr Glu Glu Arg Arg Arg Tyr Ile 85 90 95Val Arg Thr Thr Phe Leu Tyr 100 87 156 PRT Pinus radiata 87Met Ala Phe Pro Cys Phe Tyr Phe Pro Phe Arg Ile Leu Phe Phe Leu 1 5 10 15Phe Val Cys Ser Phe Ser Phe Ser Phe Ser Phe Ser Ala His Lys Phe 20 25 30His Asp Gly Arg Lys Trp Leu Leu Ser Phe Lys Ile Asp Ile Thr Asn 35 40 45Asp Pro His Ala Ser Met Ala Asn Trp Ser Pro Ala Val His Leu Cys 50 55 60Asn Trp Thr Ala Val Thr Cys Ser Arg Arg His Ala Asp Arg Val Val65 70 75 80Ser Leu Asp Leu Ser Gly Met Asp Leu Ser Gly Ser Ile Ser Pro Ser 85 90 95Leu Gly Asn Leu Ser Phe Leu His Thr Leu Asn Leu Ser Ala Asn Ala 100 105 110Leu His Gly His Ile Pro Pro Gln Leu Gly Arg Leu Phe Arg Leu Arg 115 120 125Asn Leu Trp Leu Arg Asn Asn Phe Leu Gln Gly Asn Ile Pro Thr Glu 130 135 140Phe Ala Ser Leu Lys His Leu Gln Gln Leu Tyr Leu145 150 155 88 98 PRT Eucalyptus grandis 88Met Arg Phe Ser Ser Leu Lys Arg Arg Arg Trp Ile Met Arg Thr Ala 1 5 10 15Ile Ile Ile Thr Ser Leu Met Cys Cys Ser Ser Ala Arg Asp Ser Met 20 25 30Thr Leu Ser Ser Pro Leu Ser Asp Glu His Gly Asp Thr Leu Val Ser 35 40 45Asp Gly Gly Thr Phe Gln Leu Gly Phe Phe Ser Pro Asn Gly Ser Ser 50 55 60Gly Ser Asp His Arg Arg Tyr Leu Gly Ile Trp Tyr Tyr Asp Ser Asp65 70 75 80Pro Gln Thr Val Val Trp Val Ala Asn Arg Asp His Pro Val Leu Asp 85 90 95Val Thr 89 154 PRT Eucalyptus grandis 89Met Gly Leu Ala Arg Leu Leu Leu Val Gly Phe Val Leu Leu Val Leu 1 5 10 15Gly Ser Arg Ala Leu Val Cys Gly Asn Ala Glu Leu Glu Ala Leu Met 20 25 30Glu Ile Lys Ala Ser Leu Asp Pro Glu Asn Lys Val Leu Thr Ser Trp 35 40 45Thr Ser Asn Gly Asp Pro Cys Gly Gly Ser Phe Asp Gly Val Ala Cys 50 55 60Asn Glu His Gln Lys Val Ala Asn Ile Ser Leu Gln Gly Lys Gly Leu65 70 75 80Ser Gly Lys Val Pro Pro Ala Val Ala Gln Leu Lys Cys Leu Ser Gly 85 90 95Leu Tyr Leu His Tyr Asn Tyr Leu Thr Gly Glu Ile Pro Arg Glu Ile 100 105 110Ser Ser Leu Thr Glu Leu Thr Asp Leu Tyr Leu Asp Val Asn Asn Leu 115 120 125Thr Gly Ser Ile Pro Ser Glu Ile Gly Ser Met Ala Ser Leu Gln Gly 130 135 140Glu Phe Cys Leu Leu Leu Gln Gly Gln Gly145 150 90 157 PRT Eucalyptus grandis 90Met Gly Val Lys Arg Ala Asn Ser Ser Leu Cys Cys Val Val Leu Leu 1 5 10 15Gly Leu Trp Ala Cys Val His Gly Leu Leu Ser Pro Ser Gly Val Asn 20 25 30Ile Glu Val Leu Ala Leu Met Asp Ile Arg Asn Leu Leu Val Asp Pro 35 40 45His Gln Val Leu Asn Asn Trp Asp Ala Asn Glu Val Thr Pro Cys Thr 50 55 60Trp Thr Ala Ile Thr Cys Glu Ala Asp Val Val Thr Asn Leu Glu Ile65 70 75 80Pro Arg Gln Asn Leu Ser Gly Thr Leu Ser His Ser Ile Gly Asn Leu 85 90 95Ile Asn Leu Lys Tyr Leu Phe Leu Gln Asp Asn Asn Ile Ser Gly Phe 100 105 110Ile Pro Pro Glu Leu Gly Lys Leu Gln Lys Leu Glu Met Leu Asp Ile 115 120 125Ser Ser Asn Ser Phe Ser Gly Glu Ile Pro Thr Glu Leu Ser His Leu 130 135 140Lys Asn Leu Gln Gln Leu Arg Met Asn Tyr Asn Asn Leu145 150 155 91 141 PRT Eucalyptus grandis 91Met Val Ser Ser Leu Leu Leu Ser Ser Gln Pro Asn Leu Val Val Gly 1 5 10 15Phe Leu Phe Leu Leu Leu Leu Val Asp Thr Thr Pro Phe His Gly Ala 20 25 30Gly Asp Met Ala Ser Ser Thr Ser Phe Phe Thr Ile Asn Met Thr Lys 35 40 45Ser Lys Asp Glu Val Glu Ala Leu Leu Asn Trp Lys Ser Thr Leu Asp 50 55 60Asn Tyr Ser Gln Cys Leu Leu Ser Ser Trp His Asp Asn Asn Pro Cys65 70 75 80Gly Phe Ser Gly Val Thr Cys Asp Asp Ser Lys Ala Val Asn Asn Leu 85 90 95Asn Leu Ser Asn Leu Gly Leu Arg Gly Thr Leu Asp Gly Leu Asp Phe 100 105 110Ser Cys Leu Thr Asn Leu Val Thr Phe Asp Leu Ser Tyr Asn Ala Ile 115 120 125Tyr Gly Ser Ile Pro Ser Ser Ile Gly Asn Leu Ser Lys 130 135 140 92 90 PRT Eucalyptus grandis 92Met Glu Gly Lys His Leu Val Phe Leu Thr Ile Leu Leu Leu Glu Ser 1 5 10 15Ile Cys Ser Asn Val Ser Ala Ile Pro Asn Glu Asp Lys Gln Ala Leu 20 25 30Leu Asp Phe Leu Gly Asn Val Ser Leu Ser Arg Pro Leu Asn Trp Asn 35 40 45Lys Asp Ser Ser Val Cys Arg Ser Trp Thr Gly Val Lys Cys Asn Asn 50 55 60Asp Gln Ser Arg Val Val Ala Leu Gln Leu Pro Gly Val Gly Ile Lys65 70 75 80Gly Arg Ile Pro Pro Asn Thr Leu Ser Arg 85 90 93 198 PRT Pinus radiata 93Met Ala Tyr Lys Leu Leu Arg Phe Trp Leu Glu Phe Gln Ala Leu Leu 1 5 10 15Val Leu Phe Pro Leu Ala Leu Ser Val Ser Gln Glu Gly Leu Val Leu 20 25 30Leu Glu Val Lys Lys Glu Leu Ser Asp Pro Asn Gly Phe Leu Gly Asn 35 40 45Trp Lys Ala Glu Asp Asp Ser Pro Cys Lys Trp Arg Gly Ile Ser Cys 50 55 60Asp Gln Arg Ser Lys Ser Val Val Gly Ile Asp Leu Ser Ser Gly Gly65 70 75 80Leu Val Gly Val Phe Pro Ser Val Val Cys Asn Leu Pro Gln Leu Lys 85 90 95Asn Leu Ser Leu Gly Asp Asn Asn Ile Gly Ser Ile Leu Pro Arg Asn 100 105 110Leu Ser Met Cys Arg Gln Leu Gln Arg Leu Asn Leu Ser Gln Asn Leu 115 120 125Phe Val Gly Asn Leu Pro Asp Phe Ile Ser Glu Leu Ala Glu Leu Glu 130 135 140Tyr Leu Asp Leu Ser Ser Asn Asn Phe Ser Gly Ser Ile Pro Ala Gly145 150 155 160Ile Gly Lys Leu Pro Arg Leu Gln Val Leu Asn Leu Cys Cys Asn Leu 165 170 175Leu Asn Glu Thr Ile Pro Thr Phe Leu Gly Asn Leu Ser Asn Leu Gln 180 185 190Gln Leu Leu Leu Ala Tyr 195 94 151 PRT Pinus radiata 94Met Gly Ser Ser Ala Ser Pro Phe Met Val Leu Leu Leu Leu Ser Thr 1 5 10 15Leu Ile Leu Glu Ile Leu Pro Met Tyr Val Ala Thr Asn Arg Ser Glu 20 25 30Ala Asp Leu Gln Gly Leu Ile Ala Phe Lys Ala Ser Ile Thr Ser Asp 35 40 45Pro Leu Asn Ala Leu Ala Asp Trp Thr Ala Ser Ala His His Cys Asn 50 55 60Trp Ser Gly Val Ala Cys Asp Pro Leu His Asn Val Ile Ser Ile Ser65 70 75 80Leu Pro Glu Thr Gln Ile Gln Gly Leu Ile Ser Pro Phe Leu Ala Asn 85 90 95Ile Ser Tyr Leu Ala Ser Leu Asp Leu Arg Ser Asn Phe Phe His Gly 100 105 110Val Ile Pro Pro Gln Leu Ala Leu Cys Ser Gln Leu Ile Asp Leu Glu 115 120 125Leu Phe Asn Asn Ser Leu Thr Gly Ile Lys Leu Ile Asp Thr Val Asp 130 135 140Leu Glu Gly Gly Pro Gly Thr145 150 95 151 PRT Pinus radiata 95Met Glu Val Arg Met Ile Thr Ile Met Ile Leu Cys Ala Ala Leu Leu 1 5 10 15Cys Glu Gly Ser Gly Glu Val Asp Val Leu Met Glu Met Lys Ala Ala 20 25 30Leu Asp Pro Lys Gly Glu Ile Leu Tyr Ser Trp Val Lys Gly Gly Asp 35 40 45Pro Cys Ser Gly Thr Phe Asp Gly Val Ala Cys Asn Glu Gln Gly Lys 50 55 60Val Ala Asn Val Ser Leu Gln Gly Lys Gly Leu Ser Gly Ser Ile Pro65 70 75 80Ser Thr Ile Gly Lys Leu Lys Cys Leu Thr Gly Leu Tyr Leu His Tyr 85 90 95Asn Ser Leu Gly Gly Glu Ile Pro Arg Glu Leu Ser Asn Leu Thr Glu 100 105 110Leu Leu Asp Leu Tyr Leu Asn Val Asn Gly Leu Ser Gly Pro Ile Pro 115 120 125Lys Glu Leu Gly Ala Met Ser Ser Leu Gln Ala Leu Gln Leu Cys Cys 130 135 140Asn Lys Leu Thr Gly Pro Ile145 150 96 116 PRT Pinus radiata 96Met Asp Leu Leu Leu Leu Leu Leu Val Met Met Gly Val Ala Met Pro 1 5 10 15Thr His Ser Gln Gln Thr Gly Gly Phe Thr Ser Val Gln Arg Phe Pro 20 25 30Phe Asn Gly Arg Ser Met Met Gly Lys Pro Ser Leu Phe Pro Ser Ser 35 40 45Ile Ala Gly Tyr His Glu Lys Arg Asp Val Glu Ala Leu Leu Ser Phe 50 55 60Arg Lys Gly Ile Ile Ser Asp Pro Val Gly Ser Leu Ser Asp Trp Thr65 70 75 80Ala Asn Asn Ser His Asn Val Cys Leu Trp Asn Gly Ile Ser Cys Arg 85 90 95Pro Asn Thr Lys Arg Val Val Ser Ile Ser Leu Pro Glu Cys Leu Leu 100 105 110Asn Gly Thr Leu 115 97 155 PRT Pinus radiata 97Met Arg Glu Arg Glu Thr Lys Val Cys Ile Phe Ile Phe Cys Ile Phe 1 5 10 15Thr Ser Trp Ala Leu Val Asp Phe Gly Val Pro Ala Val Phe Ala Ala 20 25 30Gln Glu Thr Gln Ile Gln Ile Leu Leu Arg Met Lys Glu Ala Leu Glu 35 40 45Asp Pro Thr Asn Ala Leu Arg Asp Trp Asp Gly Ser Glu Asp Ser Pro 50 55 60Cys Arg Trp Arg Gly Ile Asp Cys Asn Asp Glu Gly Ala Val Thr Arg65 70 75 80Ile Gln Leu His Gly Ser Ser Leu Ser Gly Arg Ile Leu Pro Asp Ile 85 90 95Cys Asn Leu Gln Ser Leu Ile Ile Phe Glu Leu Asp Arg Asn Ser Leu 100 105 110Tyr Gly Asn Phe Pro Pro Glu Phe Ser Asn Cys Ser Arg Leu Glu Gln 115 120 125Leu Asn Leu Ser Ser Asn Leu Leu Asn Gly Ser Leu Pro Asp Leu Ser 130 135 140Lys Leu Lys Ala Leu Lys Tyr Leu Asp Leu Ser145 150 155 98 96 PRT Pinus radiata 98Met Pro Thr His Ser Gln Arg Phe Pro Phe Asn Gly Trp Ser Met Met 1 5 10 15Gly Lys Pro Ser Leu Phe Pro Ser Ser Ile Ala Gly Tyr His Glu Lys 20 25 30Arg Asp Val Glu Ala Leu Leu Thr Phe Arg Lys Gly Ile Thr Leu Asp 35 40 45Pro Tyr Gly Trp Leu Ser Asn Trp Thr Ala Asn Asn Ser His Asn Val 50 55 60Cys Leu Trp Asn Gly Ile Ser Cys Ser Pro Asn Thr Asn Arg Val Val65 70 75 80Ser Ile Ser Leu Arg Tyr Gly Arg Leu Asn Gly Thr Leu Ser Pro Tyr 85 90 95 99 154 PRT Pinus radiata 99Met Asp Leu Leu Leu Leu Met Met Met Leu Val Met Met Gly Val Ala 1 5 10 15Met Pro Thr His Ser Gln Arg Phe Pro Phe Asn Gly Arg Ser Met Met 20 25 30Gly Lys Pro Ser Leu Phe Pro Ser Ser Ile Ala Gly Tyr His Glu Lys 35 40 45Arg Asp Val Glu Ala Leu Leu Thr Phe Arg Lys Gly Ile Thr Phe Asp 50 55 60Pro His Glu Trp Leu Ser Asn Trp Thr Ala Asn Asn Ser His Asn Val65 70 75 80Cys Leu Trp Asn Gly Ile Ser Cys Arg Pro Asn Thr Lys Arg Val Val 85 90 95Ser Ile Ser Leu Pro Gln Arg Ser Leu Asn Gly Thr Leu Ser Pro Tyr 100 105 110Ile Gly Asn Leu Ser Leu Leu Gln Gln Leu Asp Leu Ser Phe Asn Ala 115 120 125Leu Ser Gly Arg Ile Pro Ala Glu Phe Gly Gln Leu Lys Ala Leu Arg 130 135 140Thr Phe Glu Val Arg His Asn Ala Leu Ser145 150 100 126 PRT Eucalyptus grandis 100Met Ala Ser Cys Gly Gly Leu Val Ser Thr Phe Leu Leu Ala Ile Phe 1 5 10 15Val Thr Gln Ile Val Glu Phe Ser His Ser Ile Ala Ser Thr Asn Val 20 25 30Ser Cys Ile Gly Val Glu Arg Glu Ala Leu Leu Lys Phe Lys His Gly 35 40 45Leu Thr Asp Pro Trp Lys Arg Leu Ser Ser Trp Thr Gly Glu Glu Cys 50 55 60Cys Lys Trp Glu Gly Val Glu Cys Asn Glu Lys Thr Gly His Val Leu65 70 75 80Lys Leu Asp Leu His Asn Pro Cys Ile Glu Glu Ile Asp Met Leu Glu 85 90 95Pro Ser Tyr Lys Cys Arg Leu Gly Gly Asn Ile Val His Ser Leu Thr 100 105 110Glu Leu Lys Tyr Leu Lys His Leu Asp Leu Ser Ile Asn Asn 115 120 125 101 128 PRT Eucalyptus grandis 101Met Cys Ser Arg Arg Val Val Ser Ser Met Glu Thr Asn Pro Ile His 1 5 10 15Leu Ile Tyr His Val Phe Phe Ile Val Ile Gly Leu Val Ser Val Ser 20 25 30Ala Ala Glu Gln Ser Ala Ser Ser Arg Lys Thr Asp Ala Glu Ala Leu 35 40 45Ile Leu Phe Lys Lys Met Ile Gln Lys Asp Pro Ser Gly Val Leu Ser 50 55 60Gly Trp Gln Leu Asp Gln Asp Leu Cys Ala Trp Tyr Gly Val Thr Cys65 70 75 80Tyr Ser Gly Arg Val Thr Gln Leu Asp Leu His Gly Gln Ser Leu Glu 85 90 95Ala Thr Met Ser Phe Asp Pro Leu Ser Ser Leu Asp Met Leu Thr Val 100 105 110Ser Ile Cys His Gln Thr Arg Ser Pro Ser Ile Gln Leu Pro Cys Phe 115 120 125 102 128 PRT Pinus radiata 102Met Asp Leu Leu Leu Leu Leu Leu Val Met Met Gly Val Ala Met Pro 1 5 10 15Thr His Ser Gln Arg Phe Pro Phe Asn Gly Arg Ser Met Met Gly Lys 20 25 30Pro Ser Leu Phe Pro Ser Ser Ile Ala Gly Tyr His Glu Lys Arg Asp 35 40 45Val Glu Ala Leu Leu Ser Phe Arg Lys Gly Ile Ile Ser Asp Pro His 50 55 60Gly Ser Leu Ser Asp Trp Thr Ala Asn Asn Ser His Asn Val Cys Leu65 70 75 80Trp Asn Gly Ile Ser Cys Arg Pro Asn Thr Asn Arg Val Val Ser Ile 85 90 95Ser Leu Pro Tyr Cys Arg Leu Ser Gly Thr Leu Ser Pro Tyr Ile Gly 100 105 110Asn Leu Ser Leu Leu Arg Tyr Leu Tyr Leu Ser Asn Asn Asp Leu Ser 115 120 125 103 153 PRT Pinus radiata 103Met Arg Ser Val Phe Arg Ile Ser Ser Leu Leu Val Ile Ser Ile Leu 1 5 10 15Val Leu Leu Thr Leu Ser Ser Ala Ile Asn Asp Asp Ala Glu Met Leu 20 25 30Leu Ala Phe Lys Ser Ala Met Ser Asp Pro Asp Gly Ala Leu Ala Gly 35 40 45Trp Thr Glu Ser Asp Ala Ala Asn Phe Cys Gly Trp Thr Gly Val Leu 50 55 60Cys Asn Glu Phe Asn Arg Thr Ser Ser Leu Asp Leu Thr Asn Met Asn65 70 75 80Leu Ser Gly Ile Ile Pro Pro Arg Thr Leu Ser Ser Leu Asp Ser Leu 85 90 95Val Asn Leu Ser Leu Ala Leu Asn Lys Phe Ser Thr Pro Phe Pro Ser 100 105 110Ala Ile Leu Asp Ile Ser Thr Leu Arg Phe Leu Asn Ile Ser Asn Asn 115 120 125Asn Phe Ser Gly Glu Ile Pro Ala Asn Ile Ser Arg Leu Val Asn Leu 130 135 140Glu Leu Leu Asp Thr Tyr Asn Asn Asn145 150 104 173 PRT Pinus radiata 104Met Ala Pro Leu Leu Gly Ile Leu Leu Leu His Ala Leu Ile Phe Ser 1 5 10 15Tyr Gly Glu Glu Ala His Val Ala Gly Ser Asn His Thr Glu Val Arg 20 25 30Ala Leu Met Ala Leu Lys Ala Gly Ile Val Asp Thr Ser Gly His Leu 35 40 45Ser Asp Trp Glu Val His Gly Asp Glu Leu Ser Ala Ser Pro Cys Ser 50 55 60Trp Thr Gly Val Phe Cys Asp Leu Glu Ser Glu Asn Val Thr Glu Leu65 70 75 80Asp Leu Ser Arg Met Asn Leu Thr Gly Thr Ile Ser Asp Glu Ile Arg 85 90 95Glu Leu Gln His Leu Lys Val Leu Asn Ile Ser Phe Asn Gln Phe Ser 100 105 110Gly Ala Phe Pro Val Val Ile Phe Asn Leu Thr Arg Leu Arg Ser Leu 115 120 125Asp Ile Asn His Asn Ser Phe Glu Gly Tyr Phe Pro Ala Gly Ile Ser 130 135 140Lys Met Lys Asn Leu Val Asn Phe Ile Ala Phe Ser Asn Ser Phe Lys145 150 155 160Gly Pro Leu Pro Leu Glu Phe Val Glu Met Leu Phe Trp 165 170 105 247 PRT Eucalyptus grandis 105Met Ala Ala Pro Ser Ser Ser Ala Ala Ser Ala Ala Val Phe Ala Leu 1 5 10 15His Ser Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Val Glu Asp Asp 20 25 30Val Leu Cys Leu Glu Gly Val Lys Arg Ser Leu Gly Asp Pro Gln Gly 35 40 45Ser Leu Ala Asp Trp Thr Phe Ala Asn Thr Ser Ala Ser His Ile Cys 50 55 60Asn Leu Asn Gly Val Ala Cys Trp Asn Leu Asn Glu Asn Arg Ile Ile65 70 75 80Ser Leu Ser Leu Thr Gly Phe Gly Val Ser Gly Gly Leu Pro Glu Ser 85 90 95Leu Lys Asn Cys His Ser Leu Gln Thr Leu Asp Leu Ser Gln Asn Lys 100 105 110Leu Asp Gly Pro Ile Pro Ala Gln Ile Cys Glu Trp Leu Pro Tyr Leu 115 120 125Val Lys Leu Asp Leu Ser Ser Asn Ser Leu Ala Gly Pro Ile Pro Ser 130 135 140Gln Ile Gly Asp Cys Lys Phe Leu Asn Asn Leu Ile Leu Asn Asp Asn145 150 155 160Lys Leu Thr Gly Pro Ile Pro Tyr Glu Val Gly Arg Leu Asp Arg Leu 165 170 175Lys Val Phe Ser Val Arg Gly Asn Asp Leu Ser Gly Ser Ile Pro Ser 180 185 190Glu Leu Ser Lys Phe Ser Ser Asp Asp Phe Ser Asp Asn Asp Asp Leu 195 200 205Cys Gly Arg Pro Leu Gly Ser Cys Gly Gly Leu Ser Lys Lys Ser Leu 210 215 220Ala Ile Ile Ile Ala Ala Gly Val Leu Gly Ala Ala Ala Ser Leu Leu225 230 235 240Leu Gly Phe Ala Leu Trp Trp 245 106 145 PRT Pinus radiata 106Met Asp Leu Leu Leu Leu Leu Val Met Met Phe Val Cys Ser Val Met 1 5 10 15Met Gly Val Ala Met Pro Thr Asp Gly Phe Thr Ser Val Glu Ser Val 20 25 30Pro Phe Asn Gly Arg Asn Arg His Lys Arg Asp Val Glu Ala Leu Leu 35 40 45Ser Phe Lys Glu Ser Ile Ile Ser Asp Pro Tyr Gly Ser Leu Thr Asn 50 55 60Trp Thr Ala Asn Asn Ser His Asn Val Cys Leu Trp Asn Gly Ile Ser65 70 75 80Cys Arg Pro Asn Thr Lys Arg Val Val Ser Ile Ser Leu Pro Glu Cys 85 90 95Trp Leu Asn Gly Thr Leu Ser Pro Tyr Ile Gly Asn Leu Ser Leu Leu 100 105 110Arg His Leu Asp Leu Ser Trp Asn Ala Leu Ser Gly Arg Ile Pro Ala 115 120 125Glu Phe Gly Gln Leu Lys Ala Leu Arg Ile Leu Asp Leu Ser Ala Ser 130 135 140His145 107 150 PRT Pinus radiata 107Met Ala Pro Ser Thr Asp Phe Ile Leu Ile Thr Ser Thr Leu Met Leu 1 5 10 15Ile Phe Val Ser Ala Asn Ala His Leu Leu His His Tyr His Glu Lys 20 25 30Ser Arg Glu Arg Leu Gln Val Asp Ile Glu Ala Leu Gln Ala Phe Lys 35 40 45Ala Ser Leu Thr Tyr Asp Pro Ser His Ala Leu Ala Asn Trp Asp Phe 50 55 60Val Ala Asn His Val Cys Asn Trp Thr Gly Val Thr Cys Asn Pro His65 70 75 80Lys Leu Arg Val Ser Ala Leu Asn Leu Tyr Asn Met Ser Leu Gln Gly 85 90 95Thr Ile Pro Pro His Leu Gly Asn Ile Ser Phe Leu Gly Val Leu Asn 100 105 110Leu Thr Leu Asn Ser Phe Ser Gly Ile Ile Pro Asn Glu Leu Gly Lys 115 120 125Leu Arg Arg Leu Lys Arg Leu Ser Leu Lys Gln Asn Gln Leu Ile Ser 130 135 140Ser Ile Pro Arg Leu Lys145 150 108 126 PRT Pinus radiata 108Met Arg Met Phe Leu Leu Leu Val Tyr Leu Phe Pro Leu Val Thr Pro 1 5 10 15Phe Ala Phe Ser Thr Val Gln Leu Ser Asn Gly Ser Asn Ala Val Asp 20 25 30Gln Glu Ala Val Leu Gly Phe Leu Ser Ala Ile Thr Asn Asp Pro Tyr 35 40 45Gln Ser Leu Pro Thr Asn Trp Lys Ser Asn Val Ser Val Cys Glu Trp 50 55 60Thr Ile Ile Lys Cys Asn Gly Ser Arg Val Val Ser Leu Asn Val Ser65 70 75 80Ser Met Gly Leu Glu Gly Thr Ile Ser Pro Leu Leu Gly Asn Leu Ser 85 90 95Phe Leu Glu Lys Leu Asp Leu Arg Asn Asn Asn Phe His Gly Pro Ile 100 105 110Pro Tyr Gln Leu Gly Ser Leu Val Arg Leu Gln Met Leu Ile 115 120 125 109 118 PRT Eucalyptus grandis 109Met Glu Ser Cys Asn Cys Val Glu Pro Gln Trp Pro Ala Asp Glu Leu 1 5 10 15Leu Met Lys Tyr Gln Tyr Leu Ser Asp Phe Phe Ile Ala Leu Ala Tyr 20 25 30Phe Ser Ile Pro Leu Glu Leu Ile Tyr Phe Val Lys Lys Ser Ala Val 35 40 45Phe Pro Tyr Arg Trp Val Leu Val Gln Phe Gly Ala Phe Ile Val Leu 50 55 60Cys Gly Ala Thr His Leu Ile Asn Leu Trp Thr Phe Ala Ile His Ser65 70 75 80Arg Thr Val Ala Tyr Val Met Thr Ile Ala Lys Val Leu Thr Ala Ala 85 90 95Val Ser Cys Ile Thr Ala Leu Met Leu Val His Ile Ile Pro Asp Leu 100 105 110Leu Ser Val Lys Thr Arg 115 110 132 PRT Pinus radiata 110Phe Val Ser Asn Ala His Phe Tyr Leu Lys Val Gln Val Lys Asp Ser 1 5 10 15Gly Cys Gly Ile Gln Pro Gln Asp Ile Pro Gln Ile Phe Thr Arg Phe 20 25 30Ile His Pro Arg Ser Gly Ser Asn Arg Gly Asn Gly Ser Gly Gly Leu 35 40 45Gly Leu Ala Ile Cys Lys Arg Phe Ile Asn Leu Met Gly Gly His Ile 50 55 60Ser Ile Glu Ser Glu Gly His Asp Lys Gly Thr Ile Val Thr Phe Val65 70 75 80Val Lys Leu Gln Lys Cys Ser Asn Ala Asn Asp Ser Ala Ala His Glu 85 90 95Ile Thr Ser Arg Ala Gln Ser Ile His Glu Ser Thr His Phe Ala Arg 100 105 110His Lys Pro Leu Ile Asp Thr Asp Arg Thr Val Pro Ser Ser Ser Gln 115 120 125Tyr Gln Arg Ser 130 111 117 PRT Pinus radiata 111Ile Leu Ser Lys Arg Trp Tyr Ala Leu Met Val Leu Met Leu Pro Ser 1 5 10 15Asp Ser Ala Arg Arg Trp His Val His Glu Leu Glu Leu Val Glu Val 20 25 30Val Ala Asp Gln Val Ala Val Ala Leu Ser His Ala Ala Ile Leu Glu 35 40 45Glu Ser Met Arg Ala Arg Asp Leu Leu Met Glu Gln Asn Val Ala Leu 50 55 60Glu Ile Ala Arg Gln Glu Ala Glu Thr Ala Ile Arg Ala Arg Asn Asp65 70 75 80Phe Leu Ala Val Met Asn His Glu Met Arg Thr Pro Met His Ala Ile 85 90 95Ile Ala Leu Ser Ser Leu Leu Gln Glu Thr Glu Leu Thr Pro Glu Gln 100 105 110Arg Ser Met Val Glu 115 112 232 PRT Pinus radiata 112Val Ala Thr Pro Ser Ser His Ser Cys Ser Ser Phe Pro Leu Leu Pro 1 5 10 15Ser Thr Thr Val Thr Thr Arg Asp Arg Ser Ala Ser Asn Ala Ser Ser 20 25 30Ser Ser Gly Thr Ser Ser Thr Cys Val Ser Thr Ser Tyr Gly Ala Ile 35 40 45Ala Thr Gly Ser Asp Asn Ala Ala Ile Glu Gly Ser Pro Pro Ala Ala 50 55 60Val Ala Ala Ala Ile Met Thr Pro Ser Ser Asp Gln His His His Leu65 70 75 80His Ala Pro Gln Pro Ile Asn Val Gly Asn Thr Cys Ala Val Ala Met 85 90 95Asp Thr Leu Ala Gln His Ser Cys Ser Ser Gly Met Ala Ser Pro Pro 100 105 110Ser Thr Pro Thr Gly Ile Thr Asn Val Ala Arg Leu Thr Met Arg Ala 115 120 125Gly Ala Gly Gly Asp Gly Asp Ser His Ala Leu Ala Leu Gly Ser Asp 130 135 140Pro Ile Ala Thr Gln Ser Pro Ser Thr Lys Arg Arg Ala Ala Ser Ala145 150 155 160Pro Trp Pro Trp Ala Tyr Thr Arg Val Pro Ile Val Ala Ala Thr Ala 165 170 175Gly Ala Leu Glu Glu Glu Gln Lys Glu Cys Ile Gln Ala Gly Met Asp 180 185 190Asp Val Leu Thr Lys Pro Ile Asp Arg Tyr Gln Leu Gln Arg Lys Leu 195 200 205Ala Arg Phe Ser Pro Arg Phe Thr Ser Leu Val Val Ala Ser Ser Ala 210 215 220Pro Ala Ser Gln Gln Ala His Gln225 230 113 166 PRT Pinus radiata 113Thr Arg Val Leu Leu Ile Asp Asp His Pro Leu Phe Arg Glu Gly Leu 1 5 10 15Ala Gly Ala Ile Gln Ala Glu Pro Asp Phe Glu Val Val Gly Gln Ala 20 25 30Gly Thr Val Asp Glu Leu Arg Gly Leu Ala Pro Gln Ile Glu Pro Asp 35 40 45Val Ala Ile Val Asp Leu Leu Met Pro Ser Val Ser Gly Ile Gly Val 50 55 60Thr Arg Glu Leu Cys Glu Leu Leu Pro Arg Cys Arg Val Leu Gly Leu65 70 75 80Ser Ala Val Val Asp Ala Ala Ala Ile Ala Glu Met Leu Arg Ala Gly 85 90 95Ala Ser Gly Phe Ala Leu Lys Thr Gln Pro Ala Pro Asp Ile Leu Asp 100 105 110Ala Val Arg Arg Thr Val Ala Gly Glu Ser Tyr Leu Pro Pro Ser Val 115 120 125Ser Arg Glu Ala Ile Asp Ala Glu Leu Ala Gly Gly Ala Pro Pro Ser 130 135 140Leu Ala Gln Leu Thr Lys Ala Arg Ala Arg Asp Leu Arg Ala Asp Asp145 150 155 160Pro Arg Leu His Gln Ser 165 114 762 PRT Pinus radiata 114Met Ile Cys Leu Gly Gly Ile Val Ile Val Ser Phe Leu Leu Gln Asn 1 5 10 15Ile Ala Met Ala Ser Glu Asp Gly Gly Ser Arg Cys Asn Cys Asp Gly 20 25 30Glu Gly Trp Trp Asn Val Glu Asn Ile Met Gln Cys Gln Met Val Ser 35 40 45Asp Phe Leu Ile Ala Leu Ala Tyr Phe Ser Ile Pro Leu Glu Leu Leu 50 55 60Tyr Phe Leu Ser Cys Ser Asn Ser Leu Pro Phe Arg Trp Val Ile Val65 70 75 80Gln Phe Gly Ala Phe Ile Val Leu Cys Gly Leu Thr His Phe Ile Asn 85 90 95Ile Trp Thr Tyr Gly Pro Gln Ser Phe His Val Met Leu Ala Leu Thr 100 105 110Ile Phe Lys Phe Leu Thr Ala Leu Val Ser Cys Ala Thr Ala Ile Thr 115 120 125Leu Val Thr Leu Ile Pro Glu Leu Leu Arg Val Lys Val Arg Glu Ile 130 135 140Phe Leu Lys Asn Lys Ala Arg Glu Leu Asp Arg Glu Val Asp Ile Val145 150 155 160Lys Arg Lys Glu Glu Thr Ser Trp His Val His Met Leu Thr Gln Glu 165 170 175Ile Arg Ser Ser Leu Asp Arg His Thr Ile Leu Asn Thr Thr Leu Ile 180 185 190Ser Leu Ala Lys Thr Leu Asn Leu Glu Asn Cys Thr Ile Trp Met Pro 195 200 205Leu Ala Asp Gly Thr Ala Met Glu Val Ser His Glu Leu Lys Arg Arg 210 215 220His Leu Gln Val Pro Leu Thr Val Pro Thr Thr Asp Pro Asp Val Lys225 230 235 240Lys Ile Met His Ser Glu Asp Ala Ile Leu Leu Ser Pro Asp Ser Ala 245 250 255Leu Gly Lys Glu Ser Asn His His Trp Ser Leu Ala Gly Pro Val Ala 260 265 270Ala Ile Arg Val Pro Leu Trp Lys Ala Ser Asn Phe Lys Ser Gly Ala 275 280 285Ser Val Asp Arg Glu Glu Ser Tyr Ala Ile Met Val Leu Val Leu Pro 290 295 300Cys Glu Asp Glu Arg Gln Trp Ser Ser Gln Glu Leu Tyr Ile Val Lys305 310 315 320Asp Val Ala Glu Gln Val Ala Val Ala Leu Ser His Ala Ala Val Leu 325 330 335Glu Glu Ser Gln Lys Leu Lys Ala Pro Leu Ile Asp Lys Asn Lys Thr 340 345 350Leu Gln Gln Ala Lys Gln Asp Ala Leu Arg Ala Ser Lys Ala Arg His 355 360 365Ser Phe Gln Leu Ala Met Asn Arg Glu Met Arg Leu Pro Met His Ala 370 375 380Ile Ser Ala Leu Ser Ser Ile Leu Gln Ser Ala Arg Leu Asn Val Glu385 390 395 400Gln Leu Ala Met Thr Asn Met Leu Ala Lys Ser Ser Ser Leu Leu Ser 405 410 415Thr Leu Ile Asn Asp Ile Met Asp Phe Ser Glu Leu Glu Asp Thr Ser 420 425 430Leu Val Leu Gln Leu His Pro Phe Gln Leu His Gly Met Leu Lys Asp 435 440 445Ala Ala His Leu Thr Glu Thr Met Ser Arg Ser Lys Gly Leu Leu Leu 450 455 460Asn Val Glu Ile Gly Asp Gly Met Pro Asp His Val Ile Gly Asp Glu465 470 475 480Lys Arg Ile Leu Arg Ile Ile Leu His Met Val Gly Asn Ala Ile Asn 485 490 495Ser Thr Lys Gln Gly Thr Ile Ser Ile Arg Ile Cys Val Glu Asp Arg 500 505 510Ala Glu Gly Trp Trp Asp Pro Asn Asn Arg Arg Trp Arg Pro Ser Leu 515 520 525Cys Glu Gly Phe Thr Tyr Leu Arg Phe Glu Ile Arg Thr Ser Gly Ser 530 535 540Gly Ser Ile Gln Asn Asp Asn Pro Ser Phe Leu Thr Val Val Gln Asp545 550 555 560Gly Lys Ser Asp Ser Ser Ser Ser Thr Gly Glu Gly Leu Gly Phe Ala 565 570 575Ile Cys Lys Lys Phe Val Gln Leu Met His Gly Asn Ile Trp Leu Glu 580 585 590Pro Asn Ser Lys Gly Glu Gly Ser Val Val Thr Phe Leu Ile Arg Val 595 600 605Gln Leu Gln Thr Ser Thr Ala Asn Lys His Trp Leu Ser Pro Asp Glu 610 615 620Lys Ile Tyr Lys Ser Ser Phe Lys Gly Leu Lys Val Leu Val Ala Asp625 630 635 640Asp Asn Asn Val Ser Arg Ser Val Thr Arg Arg Leu Leu Gln Glu Leu 645 650 655Gly Cys Gln Thr Thr Glu Val Asp Ser Gly Tyr Arg Cys Leu Met Thr 660 665 670Leu Leu Gln Ser Gly Ser Ala Phe Gln Leu Val Phe Leu Glu Val Cys 675 680 685Leu Ala Gln Met Asp Gly Tyr Glu Val Ala Phe Arg Ile Arg Gln Lys 690 695 700Phe Arg Ser Arg Asn Arg Pro Leu Val Val Ala Leu Thr Ala Ser Thr705 710 715 720Asp Lys Glu Thr Met Glu Arg Cys Leu Gln Thr Gly Met Asp Gly Val 725 730 735Ile Arg Lys Pro Val Thr Leu Arg Glu Met Ser Asn Glu Leu Phe Lys 740 745 750Ile Val His Gln Thr Asn Asn Ile His Glu 755 760 115 158 PRT Eucalyptus grandis 115Thr Met Arg Ala Lys Gln Met Leu Ala Thr Met Ser His Glu Ile Arg 1 5 10 15Ser Pro Leu Ala Gly Val Val Ser Met Ala Glu Ile Leu Ala Gln Thr 20 25 30Arg Leu Asp His Glu Gln Arg Gln Leu Leu Asp Val Met Leu Ser Ser 35 40 45Gly Asp Leu Val Leu Gln Leu Ile Asn Asp Ile Leu Asp Leu Ser Lys 50 55 60Val Glu Ser Gly Val Met Lys Leu Glu Ala Thr Lys Phe Arg Pro Arg65 70 75 80Glu Val Val Lys His Val Leu Gln Thr Ala Ala Ala Ser Leu Arg Lys 85 90 95Ile Leu Thr Leu Glu Gly His Val Ala Asp Asp Val Pro Ile Glu Val 100 105 110Ile Gly Asp Val Leu Arg Ile Arg Gln Ile Leu Thr Asn Leu Ile Ser 115 120 125Asn Ala Ile Lys Phe Thr His Glu Gly Lys Val Gly Ile Asn Leu Tyr 130 135 140Val Val Pro Glu Pro Ser Val Glu Lys Thr Glu Glu Cys Pro145 150 155 116 170 PRT Eucalyptus grandis 116Lys Ile Glu Ile Glu Ala Val Gln Phe Asp Leu Arg Ala Ile Leu Asp 1 5 10 15Asp Val Leu Ser Leu Phe Ser Gly Lys Ser Gln Glu Lys Arg Val Glu 20 25 30Leu Ala Val Tyr Ile Ser Glu Asn Val Pro Glu Lys Leu Ile Gly Asp 35 40 45Pro Gly Arg Phe Arg Gln Ile Ile Thr Asn Leu Met Gly Asn Ser Ile 50 55 60Lys Phe Thr Glu Lys Gly His Ile Leu Val Thr Val His Leu Val Asp65 70 75 80Glu Val Met Asn Ser Thr Asp Ala Glu Met Glu Ser Ala Thr Arg Ser 85 90 95Thr Leu Ser Gly Phe Pro Val Pro Asp Arg Arg Leu Ser Trp Ala Lys 100 105 110Phe Arg Thr Phe Ser Gln Glu Gly Pro Ala Ser Pro Val Pro Ser Ser 115 120 125Phe Ser Asn Pro Ile Asn Leu Ile Ile Ser Val Glu Asp Thr Gly Ile 130 135 140Gly Ile Pro Pro Glu Ala Gln Pro Arg Val Phe Thr Arg Phe Met Gln145 150 155 160Val Gly Pro Ser Ile Ser Arg Thr His Gly 165 170 117 599 PRT Eucalyptus grandis 117Leu Glu His Lys Ile Ser Ile Ser Ser Leu Lys Thr Lys Leu Lys Gln 1 5 10 15His Gly Ser His Ala Arg Arg Ala Ser Lys Lys Asp Asn Lys Val Thr 20 25 30Leu Trp Phe Glu Val Asp Asp Thr Gly Cys Gly Ile Asp Pro Ser Lys 35 40 45Trp Glu Ser Val Phe Glu Ser Phe Glu Gln Ala Asp Pro Ser Thr Thr 50 55 60Arg Leu His Gly Gly Thr Gly Leu Gly Leu Cys Ile Val Arg Thr Leu65 70 75 80Val Asn Lys Met Gly Gly Glu Ile Lys Val Ile Lys Lys Asn Gly Pro 85 90 95Gly Thr Leu Met Arg Leu Tyr Leu Leu Leu Asn Ala Pro Val Asp Gly 100 105 110Thr Glu His Asn Cys Ser Val Asp Tyr Ala Val His Asn Ile Arg Val 115 120 125Leu Leu Ala Gln His Gly Ser Thr Gly Arg Phe Ile Met Ser Gly Trp 130 135 140Leu Arg Arg Asn Gly Val Ser Thr Leu Glu Ala Ser Gly Trp Asn Glu145 150 155 160Leu Thr Gln Ile Leu Gln Glu Leu Tyr Gln Gly Arg Asn Ser Gly Ala 165 170 175Pro Tyr Arg Thr Val Asn Thr Glu His Ala His Glu Leu Pro Arg Ser 180 185 190Glu Val Thr Thr Phe Asp Asp Ile Gln Ser Glu Ile Leu Ile Ile Val 195 200 205Val Asp Ile Glu Leu Leu Asp Leu Asn Thr Asp Ile Trp Lys Glu Gln 210 215 220Leu Asn Phe Leu Asp Lys Tyr His Arg Lys Ala Lys Phe Ala Trp Met225 230 235 240Leu Asn His Asp Thr Phe Asn Ala Ile Lys Val Glu Leu Arg Arg Lys 245 250 255Gly His Met Leu Met Val Asn Lys Pro Leu Tyr Lys Ala Lys Met Ile 260 265 270Gln Ile Leu Asp Ala Ala Ile Lys Glu Arg Asn Ser Glu Leu Leu Lys 275 280 285Arg Ala Ser Asn Ser Ser Lys Ser Met Asn Lys Glu Glu Asp Leu His 290 295 300Glu Cys Leu Glu Ile Asp Ser Glu His Tyr Glu Gly Ala Ser Ser Asp305 310 315 320Glu Leu Asp Thr Val Glu Thr Ser Arg Ser Gly Cys Thr Asn Thr Ser 325 330 335Pro Gly Glu Gln Lys Gln Gln Glu Gly Ile Lys Thr Pro Pro Ala Leu 340 345 350Gln His Arg Thr Ser Asn Tyr His Ser Phe Asn Ser Thr Leu Leu Ser 355 360 365Ser Asp Tyr Asn Asn Leu Gly Asn Lys Glu Glu Ala Cys Pro Thr Ser 370 375 380Pro Pro Leu Asp His Pro Asp Asn Ala Glu Gly Arg Phe Lys Cys Thr385 390 395 400Arg Ser Val Phe Ser Ser Lys Glu Lys Glu Asp Gly Asn Ser Glu Ala 405 410 415Gln Glu Gln Leu Leu Ile Ser Lys Arg Pro Pro Ala Lys Val Asp Ser 420 425 430Cys Ser Ser Lys Glu Leu Asp Gln Lys Gly Ser Leu Glu Gly Leu Cys 435 440 445Ile Leu Leu Ala Glu Asp Thr Pro Val Leu Gln Arg Val Ala Thr Ile 450 455 460Met Leu Glu Lys Leu Gly Ala Lys Val Ile Ala Val Gly Asp Gly Leu465 470 475 480Gln Ala Val Asn Ala Leu Asn Ser Ser Leu Asp Val Asp Ala Glu Asp 485 490 495Phe Arg Thr Thr Leu His Leu Gln Asn Ala Asn Arg Met Pro Gln Ala 500 505 510Gly Thr Arg Ser Trp Gln Pro Tyr Asp Leu Ile Leu Met Asp Cys Gln 515 520 525Met Pro Gln Met Asp Gly Tyr Glu Ala Thr Lys Ala Ile Arg Arg Ser 530 535 540Glu Ala Gly Ser Gly Leu His Ile Pro Ile Val Ala Leu Thr Ala His545 550 555 560Ala Met Ser Ser Asp Glu Ala Lys Cys Leu Glu Val Gly Met Asp Ala 565 570 575Tyr Leu Thr Lys Pro Ile Asp Tyr Lys Leu Met Val Ser Thr Ile Leu 580 585 590Ser Leu Thr Lys Gly Val Asn 595 118 153 PRT Eucalyptus grandis 118Leu Ser Thr Gly Ala Ser Ser Ala Thr Thr Gly Met Ala Ser Asn Gly 1 5 10 15Leu Val Ser Pro Arg Arg Arg Ser Ser Gly Gln Phe Asp Gly Ser Asp 20 25 30Pro Ser Pro Cys Gly Ser Glu Glu Val His Val Leu Ala Val Asp Asp 35 40 45Ser Leu Val Asp Arg Lys Val Ile Glu Arg Leu Leu Lys Ile Thr Ser 50 55 60Cys Lys Val Thr Ala Val Asp Ser Gly Leu Arg Ala Leu Arg Tyr Leu65 70 75 80Gly Leu Asp Glu Glu Lys Thr Ala Gly Asp Phe Asn Gly Leu Lys Val 85 90 95Asp Met Ile Ile Thr Asp Tyr Cys Met Pro Gly Met Thr Gly Tyr Glu 100 105 110Leu Leu Lys Lys Ile Lys Glu Ser Ser Ala Leu Arg Glu Ile Pro Val 115 120 125Val Ile Met Ser Ser Glu Asn Val Leu Ala Arg Ile Asp Arg Cys Met 130 135 140Glu Glu Gly Ala Glu Asp Phe Ile Val145 150 119 190 PRT Eucalyptus grandis 119Ile Ile Ser Ser Cys Ala Ser Ser Val Lys Thr Gly Met Ala Arg Asn 1 5 10 15Gly Val Ala Ser Trp Arg Arg Arg Ser Ser Asp Gln Phe Asp Asp Pro 20 25 30Ser Pro Cys Gly Ser Glu Asp Val His Val Leu Ala Val Asp Asp Ser 35 40 45Leu Val Asp Arg Lys Val Ile Glu His Leu Leu Lys Ile Ser Ser Cys 50 55 60Lys Val Thr Ala Val Asp Ser Gly Ile Arg Ala Leu Gln Phe Leu Gly65 70 75 80Leu Asp Glu Glu Lys Ala Ala Gly Asp Phe Asn Gly Leu Lys Val Asp 85 90 95Leu Ile Ile Thr Asp Tyr Cys Met Pro Gly Met Thr Gly Tyr Glu Leu 100 105 110Leu Lys Lys Ile Lys Glu Ser Ser Ala Leu Arg Glu Ile Pro Val Val 115 120 125Ile Met Ser Ser Glu Asn Val Leu Ala Arg Ile Asp Arg Cys Leu Glu 130 135 140Glu Gly Ala Glu Asp Phe Ile Val Lys Pro Val Lys Leu Ser Asp Val145 150 155 160Lys Arg Leu Arg Asp Phe Met Thr Arg Asp Val Gly Asp Arg Val Arg 165 170 175Ser Asp Gly Glu Gly Thr Thr His Lys Arg Lys Leu Gln Glu 180 185 190 120 206 PRT Pinus radiata 120Ala Ala Ala Ser Thr Glu Asp Thr Gly Ile Gly Ile Pro Leu Pro Ala 1 5 10 15Gln His Arg Val Phe Thr Pro Phe Met Gln Ala Asp Ser Ser Thr Ser 20 25 30Arg Thr Tyr Gly Gly Thr Gly Ile Gly Leu Ser Ile Ser Arg Cys Leu 35 40 45Ile Glu Leu Met Gly Gly Glu Ile Arg Phe Ile Ser Arg Pro Gly Ile 50 55 60Gly Ser Thr Phe Ser Phe Thr Ala Leu Phe Lys Val Gly Gln Ala Gly65 70 75 80Ala Asp Gly Asp Gly Asp Leu Leu Arg Gly Ala Arg Leu Pro Thr His 85 90 95Phe Lys Gly Met Lys Ala Leu Val Leu Asp Gly Asn Pro Val Cys Ser 100 105 110Leu Val Thr Lys Tyr His Leu Gln Arg Phe Gly Ile Glu Val Asp Ser 115 120 125Ile Thr Ser Ser Lys Val Ala Leu Ser Met Leu Asn Gly Met Asp Gly 130 135 140Phe Pro Thr Glu Gly Cys Ser Val Lys Asp Gly Ile Asp Met Val Leu145 150 155 160Ile Glu Lys Asp Ala Trp Gly Ser Arg His Trp His Leu Ile Ser Phe 165 170 175Ala Ser Thr Ser Arg Ser Leu Ser Lys Arg Thr Leu Ser Thr Val Lys 180 185 190Gly Phe Ile Lys Asp Asp Ser Phe Gly Tyr Ile Ala Asp Ser 195 200 205 121 123 PRT Pinus radiata 121Ser Lys Gly Leu Leu Lys Ile Ile Leu Leu Ala Thr Ser Leu Thr Ala 1 5 10 15Glu Glu Thr Gln Lys Ala Lys Ala Ala Gly Phe Ala Glu Thr Val Ile 20 25 30Leu Lys Pro Leu Arg Ala Ser Val Phe Ala Val Arg Leu Gln Leu Ala 35 40 45Leu Gly Phe Cys Tyr Arg Arg Glu His Leu Arg Glu Pro Leu Lys Thr 50 55 60Ser Ser Pro Leu Ser Asn Val Leu Ser Gly Lys Gly Ile Leu Val Val65 70 75 80Asp Asp Asn Ile Val Asn Arg Arg Val Ala Ala Gly Ala Leu Lys Lys 85 90 95Tyr Gly Ala Asn Val Ile Cys Thr Asp Gly Gly Lys Ser Ala Ile Ser 100 105 110Met Leu Arg Gln Pro His Asn Leu Ile Thr Ser 115 120 122 142 PRT Eucalyptus grandis 122Met Ala Ser Asn Gly Leu Val Ser Pro Arg Arg Arg Ser Ser Asp Gln 1 5 10 15Phe Asp Gly Ser Asp Pro Ser Pro Cys Gly Ser Glu Glu Val His Val 20 25 30Leu Ala Val Asp Asp Ser Leu Val Asp Arg Lys Val Ile Glu Arg Leu 35 40 45Leu Lys Ile Thr Ser Cys Lys Val Thr Ala Val Asp Ser Gly Leu Arg 50 55 60Ala Leu Arg Tyr Leu Gly Leu Asp Glu Glu Lys Thr Ala Gly Asp Phe65 70 75 80Asn Gly Leu Lys Val Asp Met Ile Ile Thr Asp Tyr Cys Met Pro Gly 85 90 95Met Thr Gly Tyr Glu Leu Leu Lys Lys Ile Lys Glu Ser Ser Ala Leu 100 105 110Arg Glu Ile Pro Val Val Ile Met Ser Ser Glu Asn Val Leu Ala Arg 115 120 125Ile Asp Arg Cys Met Glu Glu Gly Ala Glu Asp Phe Ile Val 130 135 140 123 146 PRT Eucalyptus grandis 123Cys Ser Phe Thr Leu Ser Ser Ser Ser Pro Leu Phe Leu Ala Val Val 1 5 10 15Leu Gly Arg Ser Pro Ile Glu Met Gly Val Thr Ala Ala Ser Gln Phe 20 25 30His Val Leu Ala Val Asp Asp Ser Leu Ile Asp Arg Lys Leu Ile Glu 35 40 45Arg Leu Leu Lys Thr Ser Ser Tyr Gln Val Thr Ala Val Asp Ser Gly 50 55 60Ser Lys Ala Leu Glu Phe Leu Gly Leu Asn Glu Gln Gln Pro Arg Asn65 70 75 80Ala Asn Ala Thr Ser Val Ser Pro Ser Tyr His His Gln Glu Ile Glu 85 90 95Val Asn Leu Ile Ile Thr Asp Tyr Phe Met Pro Glu Met Thr Gly Tyr 100 105 110Asp Leu Leu Arg Lys Ile Lys Glu Ser Asn Ser Tyr Lys Asp Val Pro 115 120 125Val Val Ile Met Ser Ser Glu Asn Val Pro Ser Arg Ile Ser Gln Cys 130 135 140Leu Glu145 124 426 PRT Eucalyptus grandis 124Gly Lys Leu Ala Val Tyr Val Ser Asp Arg Val Pro Glu Ala Val Ile 1 5 10 15Gly Asp Pro Gly Arg Phe Arg Gln Ile Ile Thr Asn Leu Val Gly Asn 20 25 30Ser Ile Lys Phe Thr His Glu Gly His Ile Phe Val Ser Val His Leu 35 40 45Leu Glu Glu Gly Cys Ser Gln His Asp Phe Arg Asp Val Glu Lys Arg 50 55 60Leu Ser Ser Asn Leu Val Glu Asp Thr Ser Asp Lys Thr Phe Asn Thr65 70 75 80Leu Ser Gly Phe Gln Val Val Asp Arg Arg Lys Ser Trp Glu Arg Phe 85 90 95Lys Lys Leu Asn Arg Ser Asp Gln Ile Asp Val Asn Glu Ser Val Glu 100 105 110Val Leu Val Thr Val Glu Asp Thr Gly Val Gly Ile Ala Arg Glu Ala 115 120 125Gln Ser Arg Ile Phe Thr Pro Phe Val Gln Ala Asp Ser Ser Thr Ser 130 135 140Arg Thr Tyr Gly Gly Thr Gly Ile Gly Leu Ser Ile Ser Lys Cys Leu145 150 155 160Val Asp Leu Met His Gly Glu Ile Gly Phe Val Ser Glu Pro Gly Thr 165 170 175Gly Ser Thr Phe Ser Phe Thr Val Pro Phe Ala Lys Cys Glu Met Asn 180 185 190Cys Leu Glu Val Lys Gly Gln Asn Tyr Asp Ser Ile Ile Ser Glu Phe 195 200 205Arg Gly Leu Arg Ala Leu Val Ile Asp Lys Arg His Ile Arg Ala Glu 210 215 220Val Ala Arg Tyr His Leu Glu Arg Leu Arg Ile Ser Val Asp Val Ala225 230 235 240Cys Ser Leu Lys Ser Ala Cys Thr Tyr Leu Ser Asn Ser Ser Ser Pro 245 250 255Arg Glu Leu Ser Asp Phe Asp Met Val Leu Ile Asp Lys Asp Val Trp 260 265 270Asp Arg Gln Thr Gly Leu Glu Leu Asn Ile Ser Leu Trp Lys His Arg 275 280 285Gln Asn Gly Ser Asn Gly Val Ser Ile Arg Pro Lys Ile Phe Leu Leu 290 295 300Ala Thr Ser Ile Ser Pro Ile Glu His Ser Glu Leu Lys Leu Ala Asn305 310 315 320Leu Val Asp Asn Val Leu Ala Lys Pro Leu Arg Leu Ser Val Leu Ile 325 330 335Ser Phe Leu Gln Glu Ala Leu Gly Asn Gly Lys Lys Arg Leu Ser Asp 340 345 350Arg Arg Lys Val Ser Thr Leu Gly Ser Leu Leu Lys Gly Arg Arg Ile 355 360 365Leu Val Val Asp Asp Asn Leu Val Asn Arg Arg Val Ala Glu Gly Ala 370 375 380Leu Lys Lys Tyr Gly Ala Ile Val Thr Cys Val Gly Ser Gly Lys Asp385 390 395 400Ala Val Ala Lys Leu Gln Pro Pro His Asp Phe Ala Ala Cys Phe Met 405 410 415Asp Leu Gln Met Pro Glu Met Asp Gly Phe 420 425 125 107 PRT Eucalyptus grandis 125Met Met Glu Ser Ser Lys Gly Phe Ser Ser Pro Arg Ser Asn Gly Phe 1 5 10 15Pro Ala Gly Leu Arg Val Leu Val Val Asp Asp Asp Pro Thr Trp Leu 20 25 30Lys Ile Leu Glu Lys Met Leu Lys Lys Cys Ser Tyr Glu Val Thr Thr 35 40 45Cys Gly Leu Ala Arg Asp Ala Leu Lys Leu Leu Arg Glu Arg Lys Gly 50 55 60Gly Tyr Asp Ile Val Ile Ser Asp Val Asn Met Pro Asp Met Asp Gly65 70 75 80Phe Lys Leu Leu Glu Leu Val Gly Leu Glu Met Asp Leu Pro Val Ile 85 90 95Met Met Ser Val Asp Gly Glu Thr Ser Arg Val 100 105 126 121 PRT Pinus radiata 126Met Val Thr Ser Arg Met Ser Ser Ala Met Arg Met Lys Lys Glu Lys 1 5 10 15Asn Ala Ala Cys Gly Glu His Gly Asp Glu Leu Val Arg Cys Asp Glu 20 25 30Met His Val Leu Ala Val Asp Asp Cys Leu Ile Glu Arg Lys Val Ile 35 40 45Glu Lys Leu Leu Lys Thr Asn Phe Phe Lys Val Thr Ser Val Asp Ser 50 55 60Ala Glu Arg Ala Leu Glu Val Leu Gly Phe His Glu Glu Gln Ser Thr65 70 75 80Cys Ala Thr Thr Asn Ala Phe Lys Val Asn Met Ile Ile Thr Asp Tyr 85 90 95Cys Met Pro Gly Met Thr Gly Tyr Asp Leu Leu Lys Lys Val Lys Glu 100 105 110Thr Lys Cys Leu Lys Glu Ile Pro Gly 115 120 127 261 PRT Pinus radiata 127Met Ala Val Ser Gln His Leu Phe Leu Ser Ala Gln Arg Leu Asn Gly 1 5 10 15Arg Glu Asp Glu Gly Ser Leu Tyr Leu Leu Arg Ala Gly Val Asn Trp 20 25 30Asp Leu Leu Ile Met Gly Val Ala Leu Val Ala Cys Leu Ala Ile Leu 35 40 45Gly Met Val Trp Lys Arg Arg Arg Thr Trp Ser Tyr Cys Glu Gly Leu 50 55 60Gln Glu Glu Asp Ala Gly Gln Arg Ala Gln Glu Thr Gln Cys Ser Lys65 70 75 80Gly Phe Met Thr Asn Val Phe His Asn Thr Arg Asp Cys Arg Ser Glu 85 90 95Gln Ile Ile Trp Asp Asp Ile His Ile Ser Ser Gln Thr Glu Thr Arg 100 105 110Ser Gln Lys Val Arg Thr Val Lys Ser Lys Ser Ser Met Ile Ser Arg 115 120 125Asp Ser Cys Ser Ser Pro Arg Arg Ile Leu Leu Val Glu Asp Thr Gln 130 135 140Ile Asn Arg Ile Ile Phe Gly Arg Val Leu Gln Ser Leu Asn Leu Tyr145 150 155 160Cys Glu Glu Ala Glu Asn Gly Lys Val Ala Val Asp Tyr Phe Lys Gln 165 170 175Gly Arg Thr Tyr Asp Leu Val Leu Met Asp Lys Glu Met Pro Val Met 180 185 190Asp Gly His Glu Ala Thr Arg Gln Leu Arg Ser Met Gly Val Lys Thr 195 200 205Pro Ile Val Ala Leu Thr Ala Asn Thr Leu Gln Ser Asp Lys Asp Leu 210 215 220Phe Phe Glu Ala Gly Val Asp Asp Phe Gln Ser Lys Pro Leu Ser Arg225 230 235 240Asp Arg Leu Val Gln Leu Leu Asp Gln Tyr Gly Val Asp Gly Cys Ala 245 250 255Gly Asn Arg Arg Gly 260 128 120 PRT Pinus radiata 128Gly Tyr Leu Gly Ser Thr Ser Ser Val Gln Pro Val Asn Phe Tyr Leu 1 5 10 15Phe Trp Phe Ile Glu Val Ala Val Pro Gln His Leu Val Leu Gly His 20 25 30Gln Tyr Leu Ser Gly Thr Glu Gly Gly Asp Gly Leu Tyr Phe Leu Arg 35 40 45Ala Gly Leu Asn Trp Val Leu Leu Ile Met Gly Val Ala Leu Val Ala 50 55 60Cys Leu Ala Ile Phe Gly Met Val Trp Lys Arg Arg Arg Thr Trp Ser65 70 75 80Tyr Cys Gly Ala Met Gln Lys Glu Asp Ala Ser Gln Arg Ala Gln Glu 85 90 95Ala Gln Cys Ser Lys Gly Cys Met Thr Asn Val Leu Pro Asn Thr Arg 100 105 110Ala Cys Arg Gly Ala His Ile Ile 115 120 129 214 PRT Pinus radiata 129Lys Arg Phe Leu Glu Gly His Glu Leu Ser Tyr Leu Arg Ala Ile Gly 1 5 10 15Val Ile Ile Leu Ser Ala Val Leu Lys Arg Arg Met Ile Leu Ala Asp 20 25 30Lys Ala Lys Ser Leu Phe Ile Ser Asn Ile Ser His Glu Leu Arg Thr 35 40 45Pro Leu His Gly Ile Leu Ala Ala Ala Glu Leu Leu Gly Asp Ser Pro 50 55 60Leu Asn His Ser Gln Leu Ser Phe Leu Glu Thr Val Gln Ala Cys Gly65 70 75 80Thr Ser Leu Val Glu Thr Val Asn His Val Leu Asp Phe Thr Lys Leu 85 90 95Ser Gly Asn Ser Lys Ala Gly Gly Val Glu Lys Val Ile Val Pro Thr 100 105 110Arg Val Asp Leu Met Gln Leu Ile Glu Glu Ala Val Asp Gly Cys Trp 115 120 125Ile Gly His Arg Ala Arg Thr Ala Ile Met Gly Asp Thr Gly Ile Gly 130 135 140Ser Val Tyr Ser Pro Pro Glu Asp Leu Ser Ser Pro Lys Gln Leu Val145 150 155 160Glu Thr Val Val Asp Ile Gly Trp Arg Lys Lys Gly Trp Ser Leu Lys 165 170 175Cys Glu Lys Gly Gly Ile Arg Arg Val Leu Met Asn Val Phe Gly Asn 180 185 190Ser Leu Lys Phe Thr Thr Asn Gly Tyr Val His Val Ile Leu Arg Glu 195 200 205Leu Pro Arg Ser Gly Asp 210 130 224 PRT Eucalyptus grandis 130Met Thr Met Ala Gly Glu Ile Leu Arg Arg Gln Ser Pro Ala Glu Val 1 5 10 15Asp Leu Cys Gly Gly Ser Gly Gln Glu Leu His Val Leu Ala Val Asp 20 25 30Asp Ser Leu Val Asp Arg Lys Val Ile Glu Lys Leu Leu Lys Arg Leu 35 40 45Ser Cys Lys Val Thr Ala Val Asp Ser Gly Leu Arg Ala Leu Gln Phe 50 55 60Leu Gly Leu Asp Gly Glu Lys Ser Ser Val Gly Leu Asp Asp Leu Lys65 70 75 80Val Asn Leu Ile Met Thr Asp Tyr Ser Met Pro Gly Met Thr Gly Tyr 85 90 95Glu Leu Leu Lys Lys Ile Lys Glu Ser Ser Ala Phe Arg Glu Thr Pro 100 105 110Val Val Ile Met Ser Ser Glu Arg Ile Leu Ala Arg Ile Asn Arg Cys 115 120 125Leu Glu Glu Gly Ala Glu Glu Phe Leu Ala Lys Pro Val Gln Leu Ser 130 135 140Asp Val Gln Arg Leu Lys Asn Phe Val Met Gly Gly Gly Glu Val Cys145 150 155 160Pro Asp Arg Arg Ile Asn Lys Arg Arg Leu Glu Glu Asn Asn Asp Asn 165 170 175Asp Asp Asn Glu Asn His Ala Pro Ser Pro Val Ser Pro Leu Cys Ser 180 185 190Arg Asp Trp Ala Val Cys Ser Ser Ser Ser Ser Asp Ser Ser Ser Pro 195 200 205Ser Ile Ala Val Ser Ser Ser Lys Arg Leu Lys Ile His His Gln Ala 210 215 220

Claims

1. An isolated polynucleotide consisting of the sequence recited in SEQ ID NO: 9 or its complementary sequence.

2. An isolated polynucleotide consisting of the reverse sequence of a polynucleotide of claim 1.

3. A DNA construct comprising a polynucleotide of claim 1 or claim 2.

4. A transgenic cell comprising a DNA construct according to claim 3.

5. A DNA construct comprising, in the 5′-3′ direction:(a) a gene promoter sequence, (b) an open reading frame of an isolated polynucleotide of claim 1; and (c) a gene termination sequence.

6. The DNA construct of claim 5 wherein the open reading frame is in a sense orientation.

7. The DNA construct of claim 5 wherein the open reading frame is in an antisense orientation.

8. The DNA construct of claim 5 wherein the gene promoter sequence and gene termination sequences are functional in a plant host.

9. The DNA construct of claim 5 further comprising a marker for identification of transformed cells.

10. A DNA construct comprising, in the 5′-3′ direction:(a) a gene promoter sequence, (b) a untranslated region of an isolated polynucleotide of claim 1, and (c) a gene termination sequence.

11. The DNA construct of claim 10 wherein the untranslated region is in a sense orientation.

12. The DNA construct of claim 10 wherein the untranslated region is in an antisense orientation.

13. The DNA construct of claim 10 wherein the gene promoter sequence and gene termination sequences are functional in a plant host.

14. A transgenic plant cell comprising a DNA construct of any one of claims 5-13.

15. A plant comprising a transgenic plant cell according to claim 14, or fruit or seeds thereof.

16. The plant of claim 15 wherein the plant is a woody plant.

17. The plant of claim 16 wherein the plant is selected from the group consisting of eucalyptus, pine, acacia, poplar, sweetgum, teak and mahogany species.

18. A plant comprising a transgenic cell according to claim 4.