Patent Grant
8652439
Incorporated by reference in its entirety herein is a computer-readable nucleotide/amino acid sequence listing submitted concurrently herewith and identified as follows: One 32,493 byte ASCII (text) file named “Seq_List” created on Jun. 20, 2011.
The present invention relates to vaccines providing enhanced immunogenicity comprising HSP60 peptides conjugated to or mixed with a viral antigen. The present invention further identifies certain novel epitopes, compositions thereof and methods of using same for vaccination or diagnosis.
Despite remarkable achievements in the development of vaccines for certain viral infections (i.e., polio and measles), and the eradication of specific viruses from the human population (e.g., smallpox), viral diseases remain as important medical and public health problems. Indeed, viruses are responsible for several “emerging” (or re-emerging) diseases (e.g., West Nile encephalitis and Dengue fever), and viral infection is a cause of significant morbidity and mortality worldwide.
The presence of adequate T-cell help is important for the construction of potent vaccines. Vaccines that induce both helper T cells and CTLs may be more effective that those that induce CTLs only. Indeed, the importance of cooperation between CD4+ and CD8+ T cells is emphasized in the therapeutic vaccination against chronic viral infection (Zajac et al., 1998; Matloubian et al., 1994).
Classically, vaccines are manufactured by introducing killed or attenuated organisms into the host along with suitable adjuvants to initiate the normal immune response to the organisms while, desirably, avoiding the pathogenic effects of the organism in the host. The approach suffers from the well known limitations in that it is rarely possible to avoid the pathogenic response because of the complexity of the vaccine which includes not only the antigenic determinant of interest but many related and unrelated deleterious materials, any number of which may, in some or all individuals, induce an undesirable reaction in the host.
For example, vaccines produced in the classical way may include competing antigens which are detrimental to the desired immune response, antigens which include unrelated immune responses, nucleic acids from the organism or culture, endotoxins and constituents of unknown composition and source. These vaccines, generated from complex materials, inherently have a relatively high probability of inducing competing responses even from the antigen of interest.
HSP60 belongs to a family of chaperone molecules highly conserved throughout evolution; a similar HSP60 molecule is present in all cells, prokaryotes and eukaryotes. The human HSP60 molecule was formerly designated HSP65, but is now designated HSP60 in view of more accurate molecular weight information; by either designation, the protein is the same. Apparently, no cell can exist without the ability to express HSP60. Mammalian HSP60 is highly homologous to the bacterial cognates, showing about 50% amino acid identity (Jindal et al., 1989). Thus, HSP60 is shared by the host and its parasites, and is immunogenic, cross-reactive, and universally expressed in inflammation. Furthermore, HSP60 is well recognized by the immune system (Konen Waisman et al., 1999, Konen Waisman et al., 1995) and is a part of the set of self-molecules for which autoimmunity naturally exists; HSP60 is member of the immunologic homunculus (Cohen, 1992). Heat shock, IFNγ, bacterial or viral infection, and inflammation, all result in the presentation of endogenous HSP60 epitopes on MHC class II molecules leading to the activation of HSP60-specific T cells, even in healthy individuals (Anderton et al., 1993; Hermann et al., 1991; Koga et al., 1989).
European Patent EP 262 710 and U.S. Pat. No. 5,154,923 describe peptides having an amino acid sequence corresponding to positions 171-240 and 172-192, respectively, of a Mycobacterium boris BCG 64 kD polypeptide, that are useful as immunogens inducing resistance to autoimmune arthritis and similar autoimmune diseases.
PCT Patent Application No. WO 90/10449 describes a peptide designated p277 having an amino acid sequence corresponding to positions 437-460 of the human HSP65 molecule that is useful as immunogen inducing resistance to insulin dependent diabetes mellitus (IDDM). A control peptide, designated p278, corresponding to positions 458-474 of human HSP65, did not induce resistance to IDDM.
Lussow et al. (1990) showed that the priming of mice with live Mycobacterium tuberculosis var. Bovis (BCG) and immunization with the repetitive malaria synthetic peptide (NANP)40 conjugated to purified protein derivative (PPD), led to the induction of high and long-lasting titers of anti-peptide IgG antibodies. Later on, Lussow et al. (1991) showed that mycobacterial heat-shock proteins (HSP) of 65 kDa (GroEL-type) and 70 kDa (DnaK-type) acted as carrier molecules in mice, previously primed with Mycobacterium tuberculosis var. boris (bacillus Calmette-Guerin, BCG), for the induction of high and long-lasting titers of IgG against the repetitive malaria synthetic peptide (NANP)40. Anti-peptide antibodies were induced when the malaria peptide, conjugated to the mycobacterial HSP, was given in the absence of any adjuvants.
Barrios et al. (1992) have shown that mice immunized with peptides or oligosaccharides conjugated to the 70 kDa HSP produced high titers of IgG antibodies in the absence of any previous priming with BCG. The anti-peptide antibody response persisted for at least 1 year. This adjuvant-free carrier effect of the 70 kDa HSP was T cell dependent, since no anti-peptide nor anti-70 kDa IgG antibodies were induced in athymic nu/nu mice. Previous immunization of mice with the 65 kDa or 70 kDa HSP did not have any negative effect on the induction of anti-peptide IgG antibodies after immunization with HSP-peptide conjugates in the absence of adjuvants. Furthermore, preimmunization with the 65 kDa HSP could substitute for BCG in providing effective priming for the induction of anti-(NANP)40 antibodies. Finally, both the 65 kDa and 70 kDa HSP acted as carrier molecules for the induction of IgG antibodies to group C meningococcal oligosaccharides, in the absence of adjuvants, suggesting that the use of HSPs as carriers in conjugated constructs for the induction of anti-peptide and anti-oligosaccharide antibodies could be of value in the design of new vaccines for eventual use in humans.
U.S. Pat. No. 5,736,146 discloses conjugates of poorly immunogenic antigens with a synthetic peptide carrier comprising a T cell epitope derived from the sequence of human heat shock protein HSP65, or an analog thereof, said peptide or analog being capable of increasing substantially the immunogenicity of the poorly immunogenic antigen. The '146 patent discloses conjugates of a peptide corresponding to positions 458-474 and 437-453 of human or mouse HSP60 and homologs thereof with a wide variety of antigens including peptides, proteins and polysaccharides such as bacterial polysaccharide (e.g. capsular polysaccharide (CPS) Vi of Salmonella typhi), and antigens derived from HIV virus or from malaria antigen.
U.S. Pat. No. 5,869,058 discloses conjugates of poorly immunogenic antigens, e.g., peptides, proteins and polysaccharides, with a synthetic peptide carrier comprising a T cell epitope derived from the sequence of E. coli HSP65 (GroEL), or an analog thereof, said peptide or analog being capable of increasing substantially the immunogenicity of the poorly immunogenic antigen. A suitable peptide according to the invention is Pep278e, which corresponds to positions 437-453 of the E. coli HSP65 molecule.
Human cytomegalovirus (HCMV) is a ubiquitous double-stranded DNA virus from the betaherpesvirus group; it is endemic in all human populations. In North America, HCMV infects about 50% of the population outside of urban centers and up to 90% of the population within cities. HCMV disease presents two major medical problems: first, it is the most common congenital viral infection, causing birth defects including mal-development of the central nervous system; up to 25% of asymptomatic infected infants will develop neurologic sequelae. Second, HCMV becomes re-activated in immunocompromised patients.
A self-limiting acute phase of viral infection, persistent and latent phases normally characterize the pathogenesis of HCMV infection in the immunocompetent host. The clinical outcome of HCMV infection is determined by the ability of infected individuals to mount protective humoral and T-cell mediated immune responses. In immunocompromised hosts, including persons with HIV infection, cancer patients and allograft recipients, primary HCMV infection or reactivation of a latent virus results in multi-organ HCMV disease, associated with high rates of morbidity and mortality. These grave clinical consequences emphasize the need for effective HCMV vaccines to prevent not only primary infection but also to limit or prevent reactivation.
At present there is no protective vaccine available for CMV. Currently available antiviral drugs which target viral DNA replication are efficacious but exhibit significant host toxicity and a high spontaneous resistance rate.
West Nile virus is a member of the alpha-like Flaviviridae. The Flavivirus genome is a single-stranded, positive-sense RNA approximately 11 kb in length, containing a 5′ untranslated region (5′UTR); a coding region encoding the three viral structural proteins; seven nonstructural proteins, designated NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5; and a 3′ untranslated region (3′UTR). The viral structural proteins include the capsid (C), premembrane/membrane (prM) and envelope (E) proteins. The structural and nonstructural proteins are translated as a single polyprotein. The polyprotein is then processed by cellular and viral proteases.
West Nile virus affects birds as well as reptiles and mammals, together with man. The West Nile virus is transmitted to birds and mammals by the bites of certain mosquitoes (e.g. Culex, Aedes, Anopheles). Direct transmission may happen from WNV infected subject to healthy subject by oral transmission (prey and transmission through colostrum) and blood/organ vectored transmission. Widespread in Africa, the geographic range of WNV now also includes Australia, Europe, the Middle East, West Asia and the USA. West Nile virus can cause a harsh, self-limiting fever, body aches, brain swelling, coma, paralysis, and death.
There is no effective treatment for the disease. A number of different WNV vaccines are now in various stages of development and testing (Monath, 2001; Pletnev et al., 2003; Tesh et al., 2002; Hall et al., 2003), but presently a licensed human vaccine is not available for its prevention. The only currently effective way to provide immediate resistance to WNV is by passive administration of protective antibodies (Casadevall, 2002). Mosquito control is currently considered the practical strategy to combat the spread of disease, but effective spraying is difficult to perform in urban areas. Clearly, an effective vaccine is needed to protect at-risk populations.
There remains a need for improved vaccines conferring protection against viral infections, using isolated epitopes. Furthermore, isolated epitopes are needed for improved diagnostic tests.
The present invention provides compositions and methods suitable for vaccination against and diagnosis of viral infections. According to some aspects the present invention provides a vaccine comprising an isolated viral antigenic peptide and a peptide comprising a T cell epitope of HSP60, wherein the HSP60 peptide enhances the immunogenicity of the viral antigenic peptide by at least two fold compared to the peptide without the HSP60 peptide. In certain currently preferred embodiments the immunogenicity is enhanced by at least 4-5 fold. Novel viral peptide antigens useful in vaccination and diagnosis are also provided.
In certain embodiments the vaccine compositions comprise a T cell epitope of HSP60 suitable to enhance the immunogenicity when used as an adjuvant peptide that is mixed with the viral antigen. In alternative embodiments the vaccine comprises a T cell epitope of HSP60 suitable to enhance the immunogenicity of the viral antigenic peptide when used in conjugates where the HSP60 peptide is covalently linked to the viral antigenic peptide.
The enhanced immunogenicity of said viral antigen is measured by at least one of the following: serum titer of antibodies directed to said viral antigen; T cell proliferation in the presence of said viral antigen; cytokine secretion induced by said viral antigen; specific T cell mediated lysis of virus-infected cells; and reduction of detectable viral load.
According to another aspect, the invention provides conjugates comprising a viral antigen covalently attached to a synthetic peptide carrier comprising a T cell epitope of HSP60. According to some embodiments, the synthetic peptide carrier is the known peptide carrier p458, a Major Histocompatibility Complex (MHC) class II-restricted peptide derived from murine HSP60 (aa 458-474, also designated previously as p278m), or an analog or derivative thereof. In other embodiments, the synthetic peptide carrier is Ec27, a novel peptide derived from E. coli HSP60 (GroEL, aa 391-410).
According to the present invention, it is now disclosed that conjugates comprising a synthetic peptide carrier selected from p458 and Ec27 covalently attached to a viral antigen are unexpectedly effective in conferring immunity against viral infections. It is now demonstrated for the first time that these conjugates significantly enhance effective immunity against both DNA and RNA viruses, latent and acute infections, and when combined with CTL-, B cell- and MHC II-restricted viral epitopes.
The principles of the invention are exemplified by two model systems for viral infections. Mouse Cytomegalovirus (MCMV) infection in mice is an established model system for examining human infection with Human Cytomegalovirus (HCMV), a DNA virus which is characterized by a latent infection following a self-limiting acute phase of viral infection. West Nile virus (WNV) infection in mice serves as a model for the acute viral infection of WNV in humans.
According to a some embodiments, the present invention provides a conjugate comprising a viral antigen covalently attached to a synthetic peptide carrier comprising a T cell epitope of HSP60 in which said synthetic peptide carrier is selected from the group of peptides consisting of:
(a) NEDQKIGIEIIKRTLKI (p458h; SEQ ID NO: 1),
(b) NEDQKIGIEIIKRALKI (p458; SEQ ID NO:2),
(c) EGDEATGANIVKVALEA (p458mt; SEQ ID NO:3),
(d) NEDQNVGIKVALRAMEA (p458e; SEQ ID NO:4),
(e) an analog of p458h (SEQ ID NO: 1) that has at least 70% of the electric and hydrophilicity/hydrophobicity characteristic of human HSP60 from position 458 to position 474, said peptide or analog being capable of increasing substantially the immunogenicity of the viral antigen when the conjugate is administered in vivo, and derivatives thereof,
(f) KKARVEDALHATRAAVEEGV (Ec27; SEQ ID NO:76) and analogs, fragments and derivatives thereof.
In one embodiment, the synthetic peptide is an analog of p458h (SEQ ID NO: 1): 458NEDQKIGIEIIKRTLKI474 in which the residue E459 is either E or D; the residue D460 is either D or E; the residue K462 is either K or R or ornithine (Orn); the residue I463 is either I or L, V, M, F, norleucine (Nle) or norvaline (Nva); the residue I465 residue is either I or L, V, M, F, Nle or Nva; the residue E466 is either E or D; the residue 1467 is either I or L, V, M, F, Nle or Nva; the residue I468 is either I or L, V, M, F, Nle or Nva; the residue K469 is either K or R or Orn; the residue R470 is either R, K or Orn; the residue L472 in either L or I, V, M, F, Nle or Nva; the residue K473 is either K or R or Orn; and the residue I474 is either I or L, V, M, F, Nle or Nva.
In another aspect, there is provided a novel adjuvant peptide derived from E. coli HSP60 (GroEL) protein, useful for the compositions and methods of the invention. The novel adjuvant peptide, herein designated Ec27, has an amino acid sequence corresponding to positions 391-410 of GroEL (corresponding to accession number gi:45686198 without the first methionine residue, SEQ ID NO:83), as follows: KKARVEDALHATRAAVEEGV (SEQ ID NO:76). It is to be explicitly understood that the corresponding peptides from mammalian species are included within the scope of the present invention. The corresponding human peptide exhibits 80% homology, having the sequence set forth in SEQ ID NO:86, as follows: KKDRVTDALNATRAAVEEGI (Ec27h). Ec27 analogs, fragments, derivatives, conjugates and salts are also contemplated by the present invention.
The Ec27 peptide is now demonstrated for the first time to increase significantly the immunogenicity of a broad array of antigens, including but not limited to viral antigens, bacterial antigens and mammalian antigens, e.g., viral peptide antigens, bacterial polysaccharides and antibodies. Surprisingly Ec27 was found to increase the immunogenicity of antigens when covalently conjugated to the antigen, as well as when mixed with the antigen. Unexpectedly, Ec27 could even further increase the immunogenicity of antigens of the invention conjugated to the p458 carriers.
In another embodiment, the invention further provides vaccine compositions comprising an antigen and a peptide adjuvant having an amino acid sequence as set forth in SEQ ID NO:76 or an analog, fragment or derivative thereof. In various embodiments, the antigen is selected from the group consisting of: a peptide, a peptide derivative, a protein, a polysaccharide (e.g. a bacterial polysaccharide), and an antibody. In one embodiment, the vaccine composition comprises a conjugate of the peptide adjuvant and said antigen. In alternate embodiments, said vaccine composition comprises an admixture of said peptide adjuvant and said antigen.
In another aspect, the viral antigen used in the conjugates and compositions of the invention comprises at least one epitope selected from: a CTL epitope (a MHC I restricted T cell epitope), a B cell epitope and a MHC II restricted T cell epitope.
The viral antigen used in the conjugates of the invention may be derived from any virus of interest. In certain embodiments, the virus belongs to the herpesviridae family. In other particular embodiments, the virus belongs to the betaherpesvirus subfamily. In one particular embodiment, the viral antigen is derived from immediate early gene 1 (IE-1) protein of a virus belonging to herpesviridae. In another particular embodiment, the viral antigen comprises a CTL epitope. In another particular embodiment, the virus is CMV. In one preferred embodiment, the viral antigen is derived from immediate early gene 1 (IE-1) protein of CMV. In another preferred embodiment, the viral antigen comprises a CTL epitope.
In other embodiments, the virus belongs to the Flaviviridae family. In other particular embodiments, the virus belongs to the flavivirus genus. According to various particular embodiments, the virus is selected from the group consisting of: West Nile virus (WNV), Yellow fever virus, St. Louis encephalitis virus, Murray Valley encephalitis virus, Kunjin virus, Japanese encephalitis virus, Dengue virus type 1, Dengue virus type 2, Dengue virus type 3 and Dengue virus type 4. In one particular embodiment, the viral antigen is derived from West Nile Virus (WNV).
In one preferred embodiment, the viral antigen is derived from the envelope (E) protein of a virus belonging to the flaviviridae family. In another preferred embodiment, the viral antigen is derived from the E3 domain of said protein. In another preferred embodiment, said viral antigen comprises a B cell epitope and a MHC II restricted epitope. In another preferred embodiment, the viral antigen is derived from the WNV envelope (E) protein. In another preferred embodiment, the viral antigen is derived from the E3 domain of said protein. In another preferred embodiment, said viral antigen comprises a B cell epitope and a MHC II restricted epitope.
Other embodiments of the present invention are directed to novel isolated viral peptide antigens that may be used in conjugation with the carriers of the invention for anti viral vaccination, as well as for diagnostic purposes, as specified herein.
In another aspect, there is provided a novel peptide antigen derived from WNV E3 domain of E protein, hereby designated p15, having an amino acid sequence corresponding to positions 355-369 of the E protein. Depending on the particular strain of WNV, this novel antigen has an amino acid sequence selected from the group consisting of: LVTVNPFVSVATANS (SEQ ID NO:11) and LVTVNPFVSVATANA (SEQ ID NO:12). Other embodiments are directed to analogs, homologs, fragments and derivatives thereof. In other embodiments, the invention provides proteins, peptides and conjugates comprising said antigen. In one particular embodiment, the peptide has an amino acid sequence as set forth in any one of SEQ ID NOS:34-35 (see Table 1).
In other embodiments, there is provided a p15 homologous peptide antigen derived from the E3 domain of the envelope protein of a flavivirus selected from the group consisting of: West Nile virus (WNV), Yellow fever virus, St. Louis encephalitis virus, Murray Valley encephalitis virus, Kunjin virus, Japanese encephalitis virus, Dengue virus type 1, Dengue virus type 2, Dengue virus type 3 and Dengue virus type 4. In certain particular embodiments, the p15 homologous antigen has an amino acid sequence as set forth in any one of SEQ ID NOS:25-33 and 36-44 (see Table 1), and analogs, homologs, fragments, and derivatives thereof.
In another aspect, there is provided a second novel WNV peptide antigen derived from the E protein, herein denoted p17, having the following amino acid sequence: YIVVGRGEQQINHHWHK (SEQ ID NO:21). Other embodiments are directed to analogs, homologs, fragments, and derivatives thereof.
In other embodiments, the invention provides nucleic acid molecules encoding said novel peptide antigens, recombinant constructs comprising these nucleic acid molecules, and vectors and cells comprising them.
In another embodiment, there are provided conjugates comprising a synthetic peptide carrier of the invention and a viral antigen having an amino acid sequence as set forth in any one of SEQ ID NOS:11-12 and 34-35 and analogs, homologs, fragments and derivatives thereof covalently attached to a synthetic peptide carrier of the invention. In another embodiment, the conjugate has an amino acid sequence as set forth in any one of SEQ ID NOS:13-16, 65-66 and 77-78. In other embodiments, the conjugates of the invention comprise a viral antigen having an amino acid sequence as set forth in any one of SEQ ID NOS:25-33 and 36-44 covalently attached to a synthetic peptide carrier of the invention. In another embodiment, the conjugate has an amino acid sequence as set forth in any one of SEQ ID NOS:56-64, and 67-75. In other embodiments, the conjugates of the invention comprise a viral antigen having an amino acid sequence as set forth in SEQ ID NO:21 covalently attached to a synthetic peptide carrier of the invention. In another embodiment, the conjugate has an amino acid sequence as set forth in any one of SEQ ID NOS:23-24 and 79 (see Table 4).
In another aspect, the invention provides vaccine compositions comprising the conjugates of the invention and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent. In another aspect, the invention provides vaccine compositions comprising a viral antigen in admixture with Ec27 and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent. In another aspect, the invention provides vaccine compositions comprising the novel isolated viral peptide antigens of the invention and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
In yet another aspect, the invention provides methods for increasing the immunogenicity of a viral antigen which comprises linking the antigen to a synthetic peptide carrier of the invention.
In another aspect, the invention provides methods for immunizing a subject in need thereof against a viral infection, comprising administering to the subject an effective amount of a vaccine composition comprising a conjugate of the invention and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
The vaccine composition may be administered to said subject before the exposure of said subject to the virus or after exposure of said subject to said virus.
In another aspect, the invention provides methods comprising:
According to various embodiments, the compositions and methods of the invention are suitable for vaccinating a subject selected from a group consisting of: humans, non-human mammals and non-mammalian animals. In a preferred embodiment, the subject is human.
Other aspects of the present invention are directed to diagnostic kits and methods utilizing the novel isolated viral peptide antigens for determining the exposure of a subject to a flavivirus.
In one aspect, there is provided a diagnostic kit comprising at least one viral peptide antigen of the invention and means for detecting whether the peptide antigen is bound specifically to a suitable biological sample.
In another aspect, the invention provides methods for diagnosing exposure of a subject to a flavivirus and for diagnosing a flavivirus infection in a subject, comprising the steps of:
In certain embodiments, the kits and diagnostic methods of the invention are useful for the differential diagnosis of a flavivirus infection.
These and other embodiments of the present invention will become apparent in conjunction with the figures, description and claims that follow.
The present invention provides novel conjugates comprising a viral antigen covalently linked to a synthetic peptide carrier comprising a T cell epitope of HSP60. The synthetic peptide carrier, p458, is a MHC class II-restricted peptide derived from murine HSP60 (aa 458-474, also designated previously as p278m), or an analog or derivative thereof, which peptide or analog being capable of increasing substantially the immunogenicity of the viral antigen. In other embodiments, the carrier is Ec27, a novel peptide derived from E. coli GroEL (aa 391-410). The invention provides vaccine compositions comprising the conjugates of the invention, and methods for their use in vaccinating a subject in need thereof against a viral infection. The invention further provides novel viral peptide antigens, conjugates and vaccine compositions thereof and uses thereof in vaccination and diagnosis.
The present invention discloses unexpectedly that a vaccine composition comprising a conjugate of a viral antigen and a peptide carrier derived from HSP60 p458 or Ec27 is highly efficacious in conferring protective immunity against a viral infection in vivo. It is now demonstrated for the first time that p458 and Ec27 enhance effective immunity even for conjugates comprising antigens that are not poorly immunogenic. The peptide carriers of the invention were found to enhance the immunogenicity of the viral antigen by at least two fold compared to the peptide without the HSP60 peptide.
The present invention is based, in part, on studies of p458-viral antigen conjugate vaccination for the treatment of a chronic (latent) Cytomegalovirus (CMV) infection associated with persistence of virus in the salivary glands. A conjugate comprising 89pep, an antigen derived from murine CMV (MCMV) immediate early gene 1 protein (IE-1), fused to p458, was more effective than the 89pep in inducing 89pep-specific IFNγ secretion and specific CTL activity. The p458-89pep chimeric peptide induced sustained IFNγ secretion in the salivary gland specific to 89pep and only this immunization was associated with clearance of virus from the salivary gland.
The present invention is also based, in part, on studies of p458-viral antigen and Ec27-viral antigen conjugate vaccination against an acute viral infection of West Nile Virus (WNV). A conjugate comprising p15, a novel antigen derived from WNV envelope (E) protein, fused to p458 was capable, upon immunization, to significantly reduce the mortality associated with the infection, while immunization with p15 alone could only moderately affect the mortality rate. The conjugate was more effective than the viral antigen alone in inducing WNV-specific neutralizing antibodies as well as WNV-specific T cell proliferation and IFNγ secretion. Ec27-p15 conjugate was also more effective than p15 alone in inducing p15-specific T cell proliferation and IFNγ secretion.
Thus, the conjugates of the invention are herein demonstrated to be effective against both DNA and RNA viruses, latent and acute infections, and when combined with CTL-, B cell- and MHC II-restricted viral epitopes.
Ec27, a novel adjuvant peptide derived from E. coli HSP60 (GroEL) protein, was found to increase significantly the immunogenicity of a broad array of antigens, including but not limited to viral antigens, bacterial antigens and mammalian antigens, e.g., viral peptide antigens, bacterial polysaccharides and antibodies. Surprisingly Ec27 was found to increase the immunogenicity of antigens when covalently conjugated to the antigen, as well as when mixed with the antigen. Unexpectedly, Ec27 could even further increase the immunogenicity of antigens conjugated to the p458 carriers. Ec27 has an amino acid sequence corresponding to positions 391-410 of GroEL (corresponding to accession number gi:45686198 without the first methionine residue, SEQ ID NO:83), as follows: KKARVEDALHATRAAVEEGV (SEQ ID NO:76).
According to a first aspect, the present invention provides a conjugate comprising a viral antigen covalently attached to a synthetic peptide carrier comprising a T cell epitope of HSP60 in which said synthetic peptide carrier is selected from the group of peptides consisting of:
The active peptide carriers according to the invention are characterized as being highly charged, i.e. of strong electric properties (7 out of 17 constituent amino acid residues of p458 are either negatively or positively charged) and highly hydrophobic (6 amino acid residues). The peptide p458h is further characterized as possessing a polar negatively-charged N-terminal domain, a polar positively-charged C-terminal domain and a highly hydrophobic core. These overall features should be maintained in order to preserve efficacy. Thus, following the above general outline certain amino acids substitution will lead to active peptides. More specifically, positions 6, 8, 10, 11, 15 and 17 in the p458 peptide chain (corresponding to positions 463, 465, 467, 468, 472 and 474 of the human HSP60 molecule) can be occupied by either I or L or by other hydrophobic amino acids, natural, such as V, M, or F, or unnatural amino acids, such as norleucine (Nle) or norvaline (Nva). Positions 5, 12, 13 and 16 in the p458h chain (corresponding to positions 462, 469, 470 and 473 of the human HSP60 molecule) can be occupied by either K or R or by unnatural positively charged amino acids, such as ornithine (Orn). Interchange of E and D may also lead to active derivatives.
With respect to the peptide carriers of the invention, the term “analogs” relates to peptides obtained by replacement, deletion or addition of amino acid residues to the sequence, optionally including the use of a chemically derivatized residue in place of a non-derivatized residue, as long as they have the capability of enhancing substantially the immunogenicity of viral antigen molecules. Analogs, in the case of p458, are peptides such that at least 70%, preferably 90-100%, of the electric properties and of the hydrophobicity of the peptide molecule are conserved. These peptides can be obtained, without limitation, according to the instructions in the paragraph hereinbefore. Ec27 analogs are preferably of at least about 70%, more preferably of at least about 80-90% similarity in their amino acid sequence of Ec27. For example, the corresponding human peptide, having the sequence set forth in SEQ ID NO:86 (KKDRVTDALNATRAAVEEGI, Ec27h), exhibits 80% amino acid identity to Ec27.
The terms “covalently attached” and “conjugated” as used herein refer to a conjugate comprising an antigen and a synthetic peptide carrier linked either as a continuous fusion peptide or by means of chemical conjugation (either directly or through a spacer), using methods well known in the art.
By “substantially increasing” the immunogenicity of a viral antigen molecule it is meant to comprise both the induction of an increase in the level of antibodies (Abs) against said antigen as well as the presentation of said antibodies as mainly of the IgG isotype. Alternatively, the term may represent an increase in antigen-specific T cell response, as measured either as increased CTL activity (antigen-dependent lysis) or as increased antigen-specific T cell proliferation or cytokine secretion (e.g. Th1 cytokines such as IFNγ). Non-limitative examples for measuring the level of specific Abs and antigen-specific T cell response according to the invention are presented in the Examples hereinbelow.
In another aspect, the viral antigen comprises at least one epitope selected from: a CTL epitope (a MHC I restricted T cell epitope), a B cell epitope and a MHC II restricted T cell epitope. Methods for identifying suitable candidate epitopes are within the abilities of those of skill in the art (for example, without limitation, by using epitope prediction software).
The viral antigen used in the conjugates of the invention may be derived from any virus of interest. In certain embodiments, the virus belongs to the herpesviridae family. This family includes, but is not limited to, human viruses such as human herpesvirus 1 (HHV-1, also known as herpes simplex virus 1, HSV1), HHV-2 (HSV2), HHV-3 (Varicella-zoster virus, VSV), HHV-4 (Epstein-Barr virus, EBV), HHV-5 (cytomegalovirus, CMV), HHV-6, HHV-7 and HHV-8.
In other particular embodiments, the virus belongs to the betaherpesvirus subfamily (e.g. CMV and EBV). In another particular embodiment, the virus is CMV. In one preferred embodiment, the viral antigen is derived from immediate early gene 1 (IE-1) protein of a herpesvirus. In another preferred embodiment, the viral antigen is derived from immediate early gene 1 (IE-1) protein of a CMV. In another preferred embodiment, the viral antigen derived from IE-1 protein comprises a CTL epitope.
In other embodiments, the virus belongs to the Flaviviridae family. This family currently contains three genera, the flaviviruses (e.g. Tick-borne encephalitis viruses, Japanese encephalitis viruses, Dengue, Yellow fever and viruses such as Modoc and Uganda virus), the pestiviruses (e.g. bovine viral diarrhea, Border disease), and the hepatitis C viruses (e.g. hepatitis C virus, HCV).
In various embodiments, the virus is selected from the group consisting of: West Nile virus (WNV), Yellow fever virus, St. Louis encephalitis virus, Murray Valley encephalitis virus, Kunjin virus, Japanese encephalitis virus, Dengue virus type 1, Dengue virus type 2, Dengue virus type 3 and Dengue virus type 4. In one particular embodiment, the viral antigen is derived from West Nile Virus (WNV). In one preferred embodiment, the viral antigen is derived from the WNV envelope (E) protein. In another preferred embodiment, the viral antigen is derived from the E3 domain of said protein. In another preferred embodiment, said viral antigen comprises a B cell epitope and a MHC II restricted epitope.
In other embodiments, there is provided a novel antigen derived from WNV E3 domain of E protein, hereby designated p15, corresponding to aa 355-369 of the E protein. In various embodiments, the antigen has an amino acid sequence as set forth in any one of SEQ ID NOS: 11 and 12 (LVTVNPFVSVATANS and LVTVNPFVSVATANA, respectively). In other embodiments, the invention provides proteins, peptides and conjugates comprising said antigen. For example, without limitation, said antigen may be conjugated with a peptide or lipid carrier or adjuvant.
In another embodiment, the conjugates of the invention comprise a viral antigen having an amino acid sequence as set forth in any one of SEQ ID NOS:11 and 12 covalently attached to a synthetic peptide carrier of the invention. In another embodiment, the conjugate has an amino acid sequence as set forth in any one of SEQ ID NOS:13 (NEDQKIGIEIIKRALKILVTVNPFVSVATANS), 14 (NEDQKIGIEIIKRALKILVTVNPFVSVATANA), (NEDQKIGIEIIKRTLKILVTVNPFVSVATANS), 16 (NEDQKIGIEIIKRTLKILVTVNPFVSVATANA), 77 (KKARVEDALHATRAAVEEGVLVTVNPFVSVATANS), and 78 (KKARVEDALHATRAAVEEGVLVTVNPFVSVATANA).
Other embodiments are directed to homologs, analogs, fragments and derivatives of p15, as detailed hereinbelow.
According to certain embodiments, the invention provides p15 homologs derived from a flavivirus, and active fragments and extensions thereof, as detailed in Table 1:
However, it should be understood that the amino acid sequence of these homologous epitopes may be altered in different variants and strains of these viruses. The present invention is thus further directed to homologous peptides from different variants and strains of these viruses.
With respect to the novel viral peptide antigens of the invention, the term “analogs” relates to peptides obtained by replacement, deletion or addition of amino acid residues to the sequence, optionally including the use of a chemically derivatized residue in place of a non-derivatized residue, as long as their ability to confer immunity against a viral infection when conjugated to the carriers of the invention is retained. The term also includes homologs corresponding to amino acid sequences which are significantly related because of an evolutionary relationship, either between species (ortholog) or within a species (paralog). Peptide sequences having conserved amino acid sequence domains are examples of homologs. With respect to the novel viral peptide antigens of the invention, peptide homologs may have at least about 40% identity in their amino acid sequence, preferably at least 50%, more preferably at least about 70% and most preferably at least about 90% identity. These values reflect the short length of the peptides.
In another aspect, there is provided a second novel WNV epitope derived from the E protein, herein denoted p17, having the following amino acid sequence: YIVVGRGEQQINHHWHK (SEQ ID NO:21). Other embodiments are directed to analogs, homologs, fragments, and derivatives thereof.
In various embodiments, these peptides and homologs may be used in conjugation with the carriers of the invention. In certain particular embodiments, the conjugate has an amino acid sequence as set forth in any one of SEQ ID NOS: 23-24 and 56-75.
Peptide and Derivative Synthesis
The polypeptides and peptides of the invention may be synthesized using any recombinant or synthetic method known in the art, including, but not limited to, solid phase (e.g. Boc or f-Moc chemistry) and solution phase synthesis methods. For solid phase peptide synthesis, a summary of the many techniques may be found in: Stewart and Young, 1963; and Meienhofer, 1973. For a review of classical solution synthesis, see Schroder and Lupke, 1965.
The amino acid residues described herein are preferred to be in the “L” isomeric form. However, residues in the “D” isomeric form can be substituted for any L-amino acid residue, as long as the peptide substantially retains the desired functional property. Use of “D” amino acids may be used as is known in the art to increase the stability or half-life of the resultant peptide.
Whenever p458 and Ec27 conjugates are mentioned in the invention, also salts and functional derivatives thereof are contemplated, as long as they are able to substantially enhance the immunogenicity of the antigen molecules. Thus, the present invention encompasses polypeptides or peptides containing non-natural amino acid derivatives or non-protein side chains.
The term derivative includes any chemical derivative of the polypeptides or peptides of the invention having one or more residues chemically derivatized by reaction of side chains or functional groups. Such derivatized molecules include, for example, those molecules in which free amino groups have been derivatized to form amine hydrochlorides, p-toluene sulfonyl groups, carbobenzoxy groups, t-butyloxycarbonyl groups, chloroacetyl groups or formyl groups. Free carboxyl groups may be derivatized to form salts, methyl and ethyl esters or other types of esters or hydrazides. Free hydroxyl groups may be derivatized to form O-acyl or O-alkyl derivatives. The imidazole nitrogen of histidine may be derivatized to form N-im-benzylhistidine. Also included as chemical derivatives are those peptides, which contain one or more naturally occurring amino acid derivatives of the twenty standard amino acid residues. For example: 4-hydroxyproline may be substituted for proline; 5-hydroxylysine may be substituted for lysine; 3-methylhistidine may be substituted for histidine; homoserine may be substituted or serine; and ornithine may be substituted for lysine.
In addition, a peptide or conjugate can differ from the natural sequence of the polypeptides or peptides of the invention by chemical modifications including, but are not limited to, terminal-NH2 acylation, acetylation, or thioglycolic acid amidation, and by terminal-carboxlyamidation, e.g., with ammonia, methylamine, and the like. Peptides can be either linear, cyclic or branched and the like, which conformations can be achieved using methods well known in the art.
It is noted that both shorter active fragments derived from the viral antigens denoted as SEQ ID NOS:11-12, 21 and 25-33 and longer peptides comprising these sequences are within the scope of the present invention. Such fragments or peptides may be comprise, for example, peptides having 1-3 amino acids deleted at either termini, or addition of 1-3 amino acid residues or more from the flanking sequences of the viral protein to either termini, as long as their ability to confer immunity against a viral infection when conjugated to the carriers of the invention is retained. It is to be understood that longer peptides, e.g. up to 50 amino acids in length may also be used for vaccination according to the invention. However, shorter peptides are preferable, in one embodiment, for being easier to manufacture. Such extensions of the novel peptide antigens of the present invention are not intended to include any known protein of fragment, such as the full length E3 domain of a flavivirus. The viral antigens, according to the present invention are preferably 5-50 amino acids in length, more preferably 8-20 amino acids in length. Exemplary fragments and extensions of the p15 and homologs thereof according to the invention are presented in Table 1.
Addition of amino acid residues may be performed at either terminus of the polypeptides or peptides of the invention for the purpose of providing a “linker” by which the peptides of this invention can be conveniently bound to a carrier. Such linkers are usually of at least one amino acid residue and can be of 40 or more residues, more often of 1 to 10 residues. Typical amino acid residues used for linking are tyrosine, cysteine, lysine, glutamic and aspartic acid, or the like.
The conjugates of the invention may also be created by means of chemically conjugating a viral antigen with a p458 or Ec27 synthetic carrier peptide, using methods well known in the art.
Nucleic Acids
In another aspect, the invention provides nucleic acid molecules encoding the peptide antigens of the invention.
The nucleic acid molecules may include DNA, RNA, or derivatives of either DNA or RNA. An isolated nucleic acid sequence encoding a viral antigen or a HSP60 peptide can be obtained from its natural source, either as an entire (i.e., complete) gene or a portion thereof. A nucleic acid molecule can also be produced using recombinant DNA technology (e.g., polymerase chain reaction (PCR) amplification, cloning) or chemical synthesis. Nucleic acid sequences include natural nucleic acid sequences and homologs thereof, including, but not limited to, natural allelic variants and modified nucleic acid sequences in which nucleotides have been inserted, deleted, substituted, and/or inverted in such a manner that such modifications do not substantially interfere with the nucleic acid molecule's ability to encode a functional peptide of the present invention.
A nucleic acid molecule homolog can be produced using a number of methods known to those skilled in the art (see, for example, Sambrook et al., 1989). For example, nucleic acid molecules can be modified using a variety of techniques including, but not limited to, classic mutagenesis techniques and recombinant DNA techniques, such as site-directed mutagenesis, chemical treatment of a nucleic acid molecule to induce mutations, restriction enzyme cleavage of a nucleic acid fragment, ligation of nucleic acid fragments, polymerase chain reaction (PCR) amplification and/or mutagenesis of selected regions of a nucleic acid sequence, synthesis of oligonucleotide mixtures and ligation of mixture groups to “build” a mixture of nucleic acid molecules and combinations thereof. Nucleic acid molecule homologs can be selected from a mixture of modified nucleic acids by screening for the function of the protein encoded by the nucleic acid with respect to the induction of an anti-viral response, for example by the methods described herein.
A polynucleotide or oligonucleotide sequence can be deduced from the genetic code of a protein, however, the degeneracy of the code must be taken into account. For example, an oligonucleotide having a nucleic acid sequence: ctggtgaccgtgaatccatttgtgtctgtggccacagccaactcg (SEQ ID NO:19) encodes a p15 antigen derived from West Nile Virus E protein: LVTVNPFVSVATANS (SEQ ID NO:11). However, nucleic acid sequences of the invention also include sequences, which are degenerate as a result of the genetic code, which sequences may be readily determined by those of ordinary skill in the art. In other particular embodiments, the viral antigens of the invention are encoded by oligonucleotides having a nucleic acid sequence as set forth in any one of SEQ ID NOS:20, 22 and 45-55 (see Table 1).
The oligonucleotides or polynucleotides of the invention may contain a modified internucleoside phosphate backbone to improve the bioavailability and hybridization properties of the oligonucleotide or polynucleotide. Linkages are selected from the group consisting of phosphodiester, phosphotriester, methylphosphonate, phosphoro selenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoroanilidate, phosphoramidate, phosphorothioate, phosphorodithioate or combinations thereof.
Additional nuclease linkages include alkylphosphotriester such as methyl- and ethylphosphotriester, carbonate such as carboxymethyl ester, carbamate, morpholino carbamate, 3′-thioformacetal, silyl such as dialkyl (C1-C6)- or diphenylsilyl, sulfamate ester, and the like. Such linkages and methods for introducing them into oligonucleotides are described in many references, e.g. reviewed generally by Peyman and Ulmann, (1990).
The present invention includes a nucleic acid sequence of the present invention operably linked to one or more transcription control sequences to form a recombinant molecule. The phrase “operably linked” refers to linking a nucleic acid sequence to a transcription control sequence in a manner such that the molecule is able to be expressed when transfected (i.e., transformed, transduced or transfected) into a host cell. Transcription control sequences are sequences which control the initiation, elongation, and termination of transcription. Particularly important transcription control sequences are those which control transcription initiation, such as promoter, enhancer, operator and repressor sequences. Suitable transcription control sequences include any transcription control sequence that can function in at least one of the recombinant cells of the present invention. A variety of such transcription control sequences are known to those skilled in the art. Preferred transcription control sequences include those which function in animal, bacteria, helminth, insect cells, and animal cells.
A nucleic acid molecule of the invention may be inserted into appropriate expression vector, i.e., a vector which contains the necessary elements for the transcription and translation of the inserted coding sequence.
Vectors can be introduced into cells or tissues by any one of a variety of known methods within the art, including in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination. Such methods are generally described in Sambrook et al., (1989, 1992), in Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. 1989.
A recombinant cell of the present invention comprises a cell transfected with a nucleic acid molecule that encodes a viral antigen of the invention. A variety of expression vector/host systems may be utilized to contain and express sequences encoding the viral antigens of the invention. These include, but are not limited to, microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid, or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with virus expression vectors (e.g., baculovirus); plant cell systems transformed with virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or with bacterial expression vectors (e.g., Ti or pBR322 plasmids); or animal cell systems. The invention is not limited by the host cell employed. The expression of the construct according to the present invention within the host cell may be transient or it may be stably integrated in the genome thereof.
Vaccine Compositions and Methods Thereof
According to some aspects the present invention provides a vaccine comprising an isolated viral antigenic peptide and a peptide comprising a T cell epitope of HSP60, wherein the HSP60 peptide enhances the immunogenicity of the viral antigenic peptide by at least two fold compared to the peptide without the HSP60 peptide. In certain currently preferred embodiments the immunogenicity is enhanced by at least 4-5 fold.
In certain embodiments the vaccine compositions comprise a T cell epitope of HSP60 suitable to enhance the immunogenicity when used as an adjuvant peptide that is mixed with the viral antigen. According to certain particular embodiments, the adjuvant peptide is selected from Ec27 and analogs and derivatives thereof. In alternative embodiments the vaccine comprises a T cell epitope of HSP60 suitable to enhance the immunogenicity of the viral antigenic peptide when used in conjugates where the HSP60 peptide is covalently linked to the viral antigenic peptide. In some particular embodiments, the peptide carrier is selected from p458, Ec27 and analogs and derivatives thereof. The enhanced immunogenicity of said viral antigen is measured by at least one of the following: serum titer of antibodies directed to said viral antigen; T cell proliferation in the presence of said viral antigen; cytokine secretion induced by said viral antigen; specific T cell mediated lysis of virus-infected cells; and reduction of detectable viral load.
In another aspect, the invention provides vaccine compositions comprising the conjugates of the invention and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
In another aspect, the invention provides vaccine compositions comprising a polypeptide or peptide, said polypeptide or peptide comprising an amino acid sequence as set forth in any one of SEQ ID NOS:11-12, 21 and 25-44, and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
In one embodiment of the invention, the composition is useful for treating or preventing a viral infection in a subject in need thereof, as described herein.
The vaccine composition of the invention is administered to a subject in need thereof in an effective amount. According to the present invention, an “effective amount” is an amount that when administered to a subject results in a substantial increase in the immune response of the subject to said viral antigen, as described herein.
According certain embodiments, the subject is selected from the group consisting of humans, non-human mammals and non-mammalian animals (e.g. birds). In a preferred embodiment, the subject is human.
Pharmaceutical and veterinary compositions for use in accordance with these embodiments may be formulated in conventional manner using one or more physiologically acceptable carriers or excipients (vehicles). The carrier(s) are “acceptable” in the sense of being compatible with the other ingredients of the composition and not deleterious to the recipient thereof. The vaccine composition can be optionally administered in a pharmaceutically or physiologically acceptable vehicle, such as physiological saline or ethanol polyols such as glycerol or propylene glycol.
The polypeptides and peptides of the invention may be formulated into the vaccine as neutral or salt forms. Pharmaceutically acceptable salts include the acid addition salts (formed with free amino groups of the peptide) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids such as acetic, oxalic, tartaric and maleic. Salts formed with the free carboxyl groups may also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine and procaine.
The vaccine composition may optionally comprise additional adjuvants such as vegetable oils or emulsions thereof, surface active substances, e.g., hexadecylamin, octadecyl amino acid esters, octadecylamine, lysolecithin, dimethyl-dioctadecylammonium bromide, N,N-dicoctadecyl-N′-N′ bis(2-hydroxyethyl-propane diamine), methoxyhexadecylglycerol, and pluronic polyols; polyamines, e.g., pyran, dextransulfate, poly IC, carbopol; peptides, e.g., muramyl dipeptide, dimethylglycine, tuftsin; immune stimulating complexes; oil emulsions (including, but not limited to, oil-in-water emulsions having oil droplets in the submicron range, such as those disclosed by U.S. Pat. Nos. 5,961,970, 4,073,943 and 4,168,308); liposaccharides such as MPL® and mineral gels. The antigens of this invention can also be incorporated into liposomes, cochleates, biodegradable polymers such as poly-lactide, poly-glycolide and poly-lactide-co-glycolides, or ISCOMS (immunostimulating complexes), and supplementary active ingredients may also be employed. The protein and peptide antigens of the present invention can be coupled to albumin or to other carrier molecule in order to modulate or enhance the immune response, all as are well known to those of ordinary skill in the vaccine art.
The vaccines can be administered to a human or animal by a variety of routes, including but not limited to parenteral, intradermal, transdermal (such as by the use of slow release polymers), intramuscular, intraperitoneal, intravenous, subcutaneous, oral and intranasal routes of administration, according to protocols well known in the art. The particular dosage of the conjugate antigen will depend upon the age, weight and medical condition of the subject to be treated, as well as on the identity of the antigen and the method of administration. Suitable doses will be readily determined by the skilled artisan. A preferred dose for human intramuscular, subcutaneous and oral vaccination is between about 6 μg to about 70 mg per kg body weight, preferably between about 15 μg to about 28 mg per kg body weight, and more preferably between about 40 μg to about 7 mg per kg body weight. Adjustment and manipulation of established dosage ranges used with traditional carrier antigens for adaptation to the present vaccine is well within the ability of those skilled in the art.
In various embodiments, the vaccine composition s of the invention may be used in combination with other treatments and medicaments, e.g. anti-viral drugs. For example, a conjugate comprising a CTL epitope derived from 1E-1 protein of HCMV and a peptide carrier of the invention may be administered to HCMV infected subjects in combination with gancyclovir therapy. Doses and administration regimes of gancyclovir are known in the art.
In another aspect, the present invention is directed to the use of a conjugate of the invention for the preparation of a vaccine composition useful for conferring anti-vial immunity.
In yet another aspect, the invention provides methods for increasing the immunogenicity of a viral antigen which comprises linking the antigen to a synthetic peptide carrier of the invention.
In another aspect, the invention provides methods for immunizing a subject in need thereof against a viral infection, comprising administering to the subject an effective amount of a vaccine composition comprising a conjugate of the invention and a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
In various embodiments, the vaccine composition may be administered to said subject before the exposure of said subject to the virus or after exposure of said subject to said virus.
In another aspect, the invention provides methods comprising:
Diagnostic Kits and Methods Thereof
Other embodiments of the present invention are directed to diagnostic compositions and kits and uses thereof for the diagnosis of flavivirus infection.
The present invention provides a method for diagnosing the presence of, or exposure to a flavivirus in a patient, comprising testing said patient for the presence of anti-flavivirus antibodies or of a T cells which immunoreact with flavivirus epitopes using a peptide according to Table 1 or analogs, derivatives and salts thereof as antigen.
In one embodiment, the method comprises the steps of:
A biological specimen or sample that may be assayed for flavivirus infection may include, for example, mammalian body fluids (e.g. serum, tissue extracts, tissue fluids, mucosal secretions), in vitro cell culture supernatants, cell lysates and cells or tissue from the subject that have been cultured in cell culture (e.g. leukocyte samples such as peripheral blood mononuclear cells). Methods of obtaining a suitable biological sample from a subject are known to those skilled in the art.
In certain embodiments, the peptides and peptide compositions prepared in accordance with the present invention can be used to detect anti-flavivirus antibodies and diagnose flavivirus infection by using them as the test reagent in an enzyme-linked immunoadsorbent assay (ELISA), an enzyme immunodot assay, a passive hemagglutination assay (e.g., PHA test), an antibody-peptide-antibody sandwich assay, a peptide-antibody-peptide sandwich assay, or other well-known immunoassays. In accordance with the present invention, any suitable immunoassay can be used with the subject peptides. Such techniques are well known to the ordinarily skilled artisan and have been described in many standard immunology manuals and texts. In one particular embodiment, the immunoassay is an ELISA using a solid phase coated with the peptide compositions of the present invention. For example, such a kit for determining the presence of anti-flavivirus antibodies may contain a solid-phase immobilized peptide of the invention and a tagged antibody capable of recognizing the non-variable region of the anti-flavivirus antibody to be detected, such as tagged anti-human Fab. The kit may also contain directions for using the kit and containers to hold the materials of the kit. Any conventional tag or label may be used, such as a radioisotope, an enzyme, a chromophore or a fluorophore. A typical radioisotope is iodine-125 or sulfur-35. Typical enzymes for this purpose include horseradish peroxidase, horseradish galactosidase and alkaline phosphatase.
In other embodiments, the presence of T cells immunoreactive with flavivirus epitopes may be determined, for example, by determining T cell proliferation or cytokine secretion induced by the novel viral peptide epitopes of the invention, using methods well known in the art. Several non-limitative examples of determining T cell reactivity with peptide antigens are presented in the Examples herein. For example, a kit for diagnosing flavivirus exposure or infection by testing for the presence of a T cell which immunoreacts with flaviviral epitopes, may comprise: an antigen selected from the peptides of the invention; a suitable medium for culture of lymphocytes (T cells); and either a labeled nucleotide for the T cell proliferation test, or a cytokine, e.g., interferon-gamma, assay kit, for the cytokine test.
In various embodiments, the method may comprise the steps of:
The term “binds specifically to the biological sample” as used herein refers to occurrence of an immune reaction between a component of the biological specimen or sample (e.g. antibodies and T cells) and the viral peptide antigen having higher affinity or extent than to another antigen. For example, specific binding may be measured by determining the extent of antigen-antibody complex formation, T cell proliferation or cytokine secretion. Thus, for example, step (b) may include determining the extent of antigen-antibody complex formation, wherein an antigen-antibody complex formation level significantly higher than the level obtained for a sample obtained from a subject not previously exposed to or infected by a flavivirus is indicative of exposure of the subject to the flavivirus.
The kits and methods of the present invention may be used, in some embodiments, for the differential diagnosis of a flavivirus infection, enabling the identification of the particular flavivirus strain infecting the subject or to which the subject was exposed. For example, a biological specimen may be assayed for the presence of anti-Dengue antibodies using the peptides having an amino acid sequence as set forth in SEQ ID NOS:30-33 to determine the strain of Dengue virus infecting the subject (e.g. to distinguish between Dengue 1, 2, 3 or 4 infection).
The following examples are presented in order to more fully illustrate some embodiments of the invention. They should, in no way be construed, however, as limiting the broad scope of the invention.
A. CMV Vaccination
Materials and Methods
Mice
BALB/c female mice were purchased from Harlan Olac (Bicester, UK). Mice were maintained under specific pathogen free conditions and were allowed to adjust to the facility for 1 week before any experiments were performed. For the pathogenesis experiments, mice were used at 6 to 8 weeks of age and for the immunization experiments, mice were used at 3 weeks of age. The mouse experiments were approved by and performed according to the guidelines of the Ben Gurion University Faculty of Health Sciences Animal Safety Committee.
MCMV
The Smith strain of MCMV was obtained from the American Type Culture Collection (ATCC) (Rockville, Md.). Highly virulent salivary gland-passaged MCMV stocks were prepared as a 10% (wt/vol) homogenate of salivary gland from day 14-infected BALB/c in DMEM-10% FCS. Homogenates were clarified by low speed centrifugation, DMSO was added to final concentration of 10%, and virus stocks were stored in aliquots at −70° C. until use (Palmon et al., 1996).
MCMV titers in these salivary gland suspension (SGS) stocks were determined by a quantitative plaque assay (Rager Zisman et al., 1973). Briefly, confluent monolayers of secondary mouse embryo fibroblasts (MEF) were prepared in 24 well plates. Serial 10-fold dilutions of SGS containing MCMV were prepared in DMEM supplemented with 2% FCS. The growth medium from each well in MEF plates was aspirated, and duplicate wells were inoculated with 0.2 ml of diluted SGS. After an adsorption period of 1 hour at 37° C., monolayers were overlayed with 0.8 ml of growth medium containing 0.75% carboxymethyl cellulose (CMC), incubated for 5 days at 37° C. in a humidified 5% CO2 incubator, fixed in PBS-10% formaldehyde and stained with Crystal Violet to visualize virus plaques. Titers were expressed as log10 pfu/0.1 gr tissue. Thorough this study virus stocks containing 1.75×108 pfu/0.1 g of tissue were used.
Infection with MCMV and Virus Titers in Target Organs
To study the course of MCMV infection in naïve or immunized BALB/c mice, mice were inoculated intraperitoneally (i.p.) with 5×104 pfu of stock virus in 0.2 ml PBS. Mice were sacrificed at different time points, spleens and salivary glands (pooled 3 mice per group at each time point) were removed and 10% (wt/vol) homogenates were prepared as previously described (Palmon et al., 1996). Samples were stored at −70° C. until infectious virus titrations were performed on primary cultures of MEF.
Preparation of DNA and Amplification by PCR
DNA was extracted from naïve and infected spleens and salivary gland using QiAmp Tissue Kit (QIAGEN Inc. Chatsworth, Calif., USA), according to appropriate QiAmp protocols. DNA oligonucleotide primers were synthesized according to the published sequence of MCMV gB gene (Rapp et al., 1992). The sequence of gB sense strand primer was based on the cDNA sequence no. 2416-2443 (5′-AAG-CAG-CAC-ATC-CGC-ACC-CTG-AGC-GCC-3′ SEQ ID NO:17) and the antisense no. 2745-2772 (5′-CCA-GGC-GCT-CCC-GGC-GGC-CCG-CTC-TCG-3′ SEQ ID NO:18). This gB gene primer pair amplifying a 356 bp segment was found the most sensitive in previous studies (Palmon et al., 1996). For gene amplification, 1 μg of DNA sample was added to the reaction mixture containing 200 μM each dNTP, 100 pmol each primer, 1.0 mM MgSO4, 10 mM KCl, 10 mM (NH4)2SO4, 20 mM Tris-HCl (pH 8.8), 0.1% Triton X-100 and 2 U of vent polymerase (Biolabs) in a total reaction volume of 50 μl each. Samples were amplified for 30 cycles in an automated thermal cycler (Perkin Elmer Cetus, USA). Each cycle entailed denaturation at 94° C. for 60 sec, annealing at 68° C. for 90 sec and primer extension at 72° C. for 120 sec. PCR products were electrophoretically separated on 1.5% agarose gel, stained with ethidium bromide, and photographed. The lower limit of detection for this method under the experimental conditions was 5 femtograms of viral DNA corresponding to about 20 copies of the MCMV genome (Palmon et al., 1996).
Peptides
Peptides were prepared in the Weizmann Institute of Science (Rehovot, Israel), and in Albert Einstein College of Medicine (New-York USA). The purity of the peptides was ascertained by analytical reversed-phase HPLC and amino acid (aa) analysis. The sequences of the six peptides synthesized are: 89pep (MCMV pp 89 ie1-CTL epitope, Reddehase et al., 1989)-YPHFHPTNL (SEQ ID NO:5); p458 (the active peptide derived from mouse HSP60, Konen Waisman et al., 1999)-NEDQKIGIEIIKRALKI (SEQ ID NO:2); p458-89pep (combined)-NEDQKIGIEIIKRALKIYPHFHPTNL (SEQ ID NO:6); negative control for p458 (the p431 peptide of the mycobacterial HSP60, 442val-deleted)-EGDEATGANI-KVALEA (SEQ ID NO:7); control-89pep (combined)-EGDEATGANI-KVALEAYPHFHPTNL (SEQ ID NO:8); and TTp30-89pep (combined)-FNNFTVSFWLRVPKVSASHLEYPHFMPTNL (SEQ ID NO:9). The p30 of TT (aa 947-967) (Panina-Bordignon et al., 1989) (SEQ ID NO:10) is now being used as a carrier peptide in various vaccines (Brander et al., 1996; Keitel et al., 1999). The mycobacterial p431 peptide (442val-deleted) was used as a negative control peptide since it is homologous in sequence to mammalian p458, but did not elicit a CD4+-dependent immune response against itself or p458.
Immunization and Challenge of Mice with MCMV
The immunizing dose of each peptide was equimolar to 15 μg of p458 (Konen Waisman et al., 1999). All peptides were emulsified in incomplete Freund's adjuvant (IFA), and the volumes for intra-footpad (i.f.p.) and subcutaneous (s.c.) injections were 50 μl and 100 μl respectively. Two different protocols were used. To study the immune response of mice to the chimeric peptide (p458-89pep), groups of 6-week old to 8-week old mice were immunized once into the hind footpad with peptides emulsified in IFA. Ten days later several mice were sacrificed, and organs were harvested for IFNγ and IL-4 assays. To study the protective efficacy of the combined peptide, groups of 3-week old mice were immunized and boosted according to the following protocol: mice were immunized i.f.p. on day (−24), and boosted s.c. two weeks later on day (−10). Ten days later (day 0), mice were challenged IP with 5×104 pfu of MCMV. Mice were sacrificed on days 14, 21, and 28 after challenge, and organs were harvested for virus titrations, PCR, cytotoxic T cell and cytokine assays.
Preparation of Spleen and Salivary Gland Mononuclear Cell Cultures
Spleen pulp was extruded from the capsule in a non-tissue culture Petri dish in RPMI-1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 10 mM HEPES and 5% FCS (base-RPMI). Spleen cell suspensions were passaged through a cell strainer, washed once, treated 2 min with ACK lysing buffer (0.15M NH4Cl, 0.01 KHCO3; 2 ml/spleen) for elimination of erythrocytes, and washed twice in base-medium. Splenocytes were resuspended in RPMI-1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 10 mM HEPES, 5×10−5M β-mercaptoethanol and 10% FCS (complete RPMI) in a final concentration of 5×106 cells/ml.
Salivary gland cell suspensions were prepared by initially cutting the salivary glands into small fragments (<2 mm) in a non-tissue culture Petri dish. Fragments were treated with base-medium containing 1 mg of collagenase-dispase (Roche Diagnostics, Germany)/ml and 50 μg of DNase I (Boehringer Mannheim, Germany)/ml. After 1 h incubation in 37° C., cells were resuspended in 45% Percoll (Sigma Chemical Co., Israel), overlayed on 66% Percoll and centrifuged at 800 g for 25 min. Mononuclear cells collected at the interphase, were counted and resuspended in complete-RPMI to a final concentration of 5×106 cells/ml.
IFNγ and IL-4 ELISA Assays
Mononuclear cell cultures from spleens and salivary gland were prepared as described above. Cell suspensions were divided into 24 well plates (5×106 cells/well) and were stimulated in vitro with either 10 μg/ml of 89pep or p458 or with 5 μg/ml Concanavalin-A (Con-A). Cells were incubated for 72 h (with or w/o stimulation) at 37° C. in a humidified 5% CO2 incubator. After incubation supernatants were collected, and IFNγ and IL-4 levels were measured using indirect ELISA according to Pharmingen cytokine ELISA protocol (Pharmingen, San Diego, Calif.).
FACS Analysis of Cell Phenotypes and Intracellular IFNγ
For phenotypic analysis, spleen and salivary gland mononuclear cells of MCMV-infected and naïve mice were cultured as described above and were stained for CD8 and CD4, IFNγ and IL-5 using directly-labeled antibodies (PharMingen, San Diego, Calif.). Intracellular cell staining (ICCS) for IFNγ, IL-4 and IL-5 was performed using PharMingen's Cytofix/Cytoperm Plus kit with GolgiPlug (containing Brefeldin A) according to the manufacturer's instructions. Briefly, GolgiPlug was added to the 8 h-incubated immune cell cultures (established as described above, with or w/o peptide stimulation). After the additional 6 h of incubation in the incubator cells (minimum 106 per sample) were harvested, washed in PBS supplemented with 2% FBS and 0.09% Sodium azide, and incubated in 50 μl of FC blocker, labeled with anti-CD4 and anti-CD8 surface markers. Then cells were fixed, permeabilized and treated with anti-IFNγ, anti-IL-4 or anti-IL-5 antibodies for intracellular cytokine detection. Stained cells were immediately analyzed on a FACSCalibur flow cytometer (Becton-Dickinson, Mansfield, Mass.) and 50,000 to 100,000 events/sample were acquired and analyzed with CellQuest software.
Cytotoxic T Cell Assay
The cytotoxic activity against the MCMV 89pep was assessed in a 4-h cytotoxic assay using the CytoTox 96 non-radioactive, colorimetric-based kit (Promega, Madison, Wis.), according to manufacturer instructions. This assay is based on the quantitative measurement of lactate dehydrogenase, a stable cytosolic enzyme that is released upon cell lysis. Spleen cell suspensions from immunized mice, prepared as described above, were re-stimulated in vitro for 6 days with 89pep (10 μg/ml) and rhIL-2 (25 IU/ml) from day 2. Target cells for the lysis assay were P815 cells (mastocytoma, H-2d). P815 were either non-pulsed or pulsed with 89pep (1 μg/ml) for 2 h and then washed before incubation with effector cells. In all experiments shown, the spontaneous release was less than 25% of maximal release. Each point in a lysis assay represents the average of triplicate values. The range of the triplicates was within 5% of their mean.
MCMV infection is characterized by different kinetics and viral loads in different organs (Mercer and Spector, 1986). BALB/c mice, 6-8 weeks old, were injected i.p. with 5×104 pfu of MCMV. Mice were sacrificed on days 1, 3, 7, 14 and 28 after infection, and spleens and salivary glands were assayed for infectious virus and MCMV DNA.
In the salivary gland, virus appeared on day 7. Virus replication in this organ steadily increased, peaking by day 14 (3×108 pfu/0.1 gr tissue,
89pep is the H-2Ld-restricted YPHFMPTNL epitope of MCMV-pp 89 (Reddehase et al., 1989). We synthesized chimeric p458-89pep and compared its protective efficacy against MCMV to that induced by 89pep alone or by negative control-89pep. p458 is a MHC class II-restricted peptide derived from murine HSP60 (aa 458-474) and capable of inducing CD4+ T responses in BALB/c mice (Amir-Kroll et al., 2003). The mycobacterial HSP60 431-447 aa peptide (with a val deletion at position 442) did not elicit an immune response to itself or to p458, and thus served as a negative control peptide for immunization; control-89pep.
To investigate whether immunization with the different peptides would decrease MCMV replication in salivary glands, 3-week old BALB/c mice were immunized twice with IFA alone, 89pep, p458-89pep or control-89pep (
Peptides for vaccination were emulsified in IFA. Ten days after the last immunization, mice were challenged i.p. with 5×104 pfu of MCMV (day 0). Mice were sacrificed on days 14, 21, and 28 after challenge, and infectious virus titers and MCMV-DNA were measured by plaque and PCR assays in the salivary glands. As shown in
On day 28, however, MCMV was not detectable in the salivary glands of the p458-89pep-immunized mice (<2 log10 pfu/0.1 g). Immunization with 89pep alone showed a marginal advantage compared to IFA-immunized mice (
It is well established that clearance of MCMV during acute infection depends primarily on Th1 IFNγ secretion and protective CTL responses (Mercer and Spector, 1986; Reddehase et al., 1989). We tested whether IFNγ secretion was stimulated by 89pep from spleen (
We investigated whether immunization with p458-89pep induced 89pep-specific IFNγ secretion. Mice received a single immunization with the following peptides: p458-89pep, 89pep, p458, control-89pep or TTp30-89pep. TTp30 is a MHC class II-restricted peptide capable of inducing vigorous CD4+ T responses and IFNγ production in BALB/c mice and used as a universal adjuvant (Panina-Bordignon et al., 1989, Amir-Kroll et al., 2003). In addition, a non-vaccinated group was infected with MCMV. Ten days after immunization with the different peptides or infection, mice were sacrificed, and spleen cell and salivary gland cultures were prepared and stimulated in vitro for 3 d with 89pep or p458.
The response of spleen cell cultures to stimulation with p458 induced high levels of IFNγ in mice immunized with p458 or p458-89pep, but not in other groups; this indicates that the responses were immunologically specific (
We characterized the nature of cells secreting the IFNγ by flow cytometry. Mice were immunized once with the different peptides and an additional group was infected with MCMV. Seven days later, spleens were removed and cell suspensions were cultured for 5 days with or without 89pep. Immunization with p458-89pep followed by 5 days of re-stimulation with 89pep induced IFNγ+CD8+ T cells and no IFNγ+CD4+ T cells (
We also investigated whether the CD8+IFNγ+ cells induced by the p458-89pep were able to lyse specifically 89pep-loaded target cells. Mice were immunized once and 7 d later, spleens were harvested and re-stimulated with 89pep. Six days later, lytic activity was assayed on P815 (H-2d) loaded with the 89pep. No lytic activity was observed from the cultures of 89pep-immunized mice, but CTLs induced by p458-89pep lysed the target cells (
We found, above, that IFNγ secretion in the salivary gland was virus-specific and depended on MCMV infection (
3-week old BALB/c mice were challenged i.p. with 5×104 pfu of MCMV (day 0). On day 6, mice were immunized once with IFA alone, 89pep or p458-89pep emulsified in IFA, as described above. Mice were treated with 100 μg of the anti viral medication Gancyclovir (GCV, Roche, Basel, Switzerland) i.p. on days 1, 2, 3, 4, 8, 9, 10 and 11 after challenge. Mice were sacrificed on day 30 after challenge and MCMV-DNA was measured by a PCR assay in the salivary glands, as specified above (
As can be seen in
B. WNV Vaccination
Materials and Methods
Mice
BALB/c female mice were purchased from Harlan Olac (Jerusalem, Ill.) at the age of 14 days (10-12 g body weight). Mice were maintained under specific pathogen free conditions and were allowed to adjust to the facility for 1 week before experiments were performed. Mice were used at the age of 3-6 weeks unless otherwise stated. Age- and sex-matched animals were used as controls. Mice were maintained in isolation cages and were fed and watered ad libitum. The mouse experiments were approved and performed according to the guidelines of the Ben Gurion University, Faculty of Health Sciences, Animal Safety Committee.
Cell Cultures
The Vero cell line was derived from African Green Monkey (ATCC® number: CCL-81). The cells were grown in DMEM supplemented with 10% FCS, 1% nonessential amino acids and antibiotics. The cells were maintained in a humidified atmosphere at 37° C. in 5% CO2 and were used for growing virus stocks, virus titration and neutralization assays.
Virus, Virus Titrations and WNV Antigen
The strain of West Nile Virus (WNV) was isolated from a human case of WNV infection (Goldblum et al., 1954). Signature amino acid motifs indicate that this strain belongs to lineage I. Virus plaque assays were performed on Vero cell monolayers in 24 well plates as previously described (Ben-Nathan et al., 1996). Virus stock titers were expressed as plaque-forming units (pfu) per ml. A single virus stock containing 5×107 pfu/ml was prepared in Vero cells, stored in aliquots at −70° C., and was used throughout this study. WNV antigen (WNV Ag) was prepared as previously described (Ben-Nathan et al., 2003).
Peptides
Peptides were synthesized at Sigma-Aldrich (Rehovot, Israel). Peptide purity was ascertained by analytical reversed-phase HPLC and amino acid (aa) analysis and was assessed on >95% purity. The sequences of the six peptides synthesized are: Ep15 (derived from the E3 domain of WNV)-LVTVNPFVSVATANS, SEQ ID NO:11; p458 (the active peptide derived from mouse HSP60)-NEDQKIGIEIIKRALKI, SEQ ID NO:2; p32 (p458-Ep15 combined)-NEDQKIGIEIIKRALKILVTVNPFVSVATANS, SEQ ID NO:13; pmock, a negative control for p458 (the p431 peptide of the mycobacterial HSP60, 442val-deleted)-EGDEATGANIKVALEA, SEQ ID NO:7; p458-89pep (combined p458 and 89pep, 89pep is a nonapeptide, YPHFHPTNL SEQ ID NO:5, which consists of a MCMV pp 89 ie1-CTL epitope)-NEDQKIGIEIIKRALKIYPHFHPTNL, SEQ ID NO:6. The mycobacterial p431 peptide (442val-deleted) was used as a negative control peptide since it is homologous in sequence to mammalian p458, but did not elicit a CD4+-dependent immune response or antibodies against itself or p458.
Antibodies and Sera
Human intravenous immunoglobulin-IL (IVIG-IL): the IgG preparation from Israeli donors (IVIG-IL; OMRIGAM 5% intravenous IgG) containing 50 mg/ml IgG (total protein 5% w/v) was a gift from Omrix Biopharmaceuticals Ltd, Israel. This product has an anti-WNV antibody titer of 1:1600 by ELISA and of >1:80 by plaque-reduction neutralization testing (PRNT) (Ben-Nathan et al., 2003). Mouse WNV antiserum was prepared by intraperitoneal (IP) injection of 5-week old BALB/c mice with 1×104 pfu of WNV per mouse. Two weeks later, surviving mice were boosted with 1×104 pfu and bled 7 days later. Blood was centrifuged (4000 rpm for 7 min), and serum was collected and stored at −20° C. The antibody titer, measured by ELISA, was 1:2400. Serum from mock-injected naïve mice was used as a negative control.
Recognition of WNV-Ag and Peptides by IVIG and Mouse Sera
ELISA tests were performed according to the method described by Martin et al (Martin et al, 2000) with slight modifications. Briefly, microtiter plates were coated and incubated overnight at 4° C. with 100 μl of the different peptides (1 μg/well) or WNV antigen diluted 1:700 in coating buffer (NaHCO3, pH=9.6). After incubation, the coating buffer was decanted and the plates were washed twice in PBS containing 0.05% Tween 20 and 0.2% sodium azide (washing buffer). After blocking for 1 h with a 200 μl/wen of PBS containing 0.05% Tween 20 and 2.5% nonfat dry milk, the plates were washed 4 times in washing buffer and 100 μl of IVIG-IL or mouse sera at 1:40 dilution were added to each well (2-4 wells per sample). Negative and positive controls of human or mouse sera were tested in each plate. After incubation for 1 h at 37° C. in a humidified atmosphere, the plates were washed 5 times, and 1000 of 1:1000 diluted HRP-Streptavidin-conjugated anti-human IgG (Sigma-Aldrich) or 1:1000 diluted HRP-Streptavidin-conjugated anti-mouse IgG (SouthernBiotech, Birmingham, Ala.) respectively, was added to each well. After incubation at 37° C. for 1 h, the plates were washed 5 times and 1000 of TMB substrate (DAKO Carpinteria, Calif.) was added to each well and incubated at room temperature for 30 min. The color intensity was measured by ELISA-reader (Dynatec MR 5000) at the absorbance of 405 nm.
Lymphocyte Proliferation Assay
Splenocytes from immunized or naive mice were prepared as described above, resuspended in complete RPMI in a final concentration of 2×105 cells per well in 96-well plates and stimulated in vitro with either 10 μg/ml of different peptides, 10 μl/ml of WNV-Ag, or 5 μg/ml of Concanavalin-A (Con-A). Cell cultures were incubated at 37° C. in 5% CO2 for 5 days. At the end of incubation, 1 μCi of 3H-Thymidine (Amersham Biosciences, Buckinghamshire, England) was added to each well for 12 h. Radioactive counting was performed on a β-counter (WALLAC 1409).
Immunization of Mice with Peptides and WNV Challenge
The immunizing dose of each peptide was equimolar to 15 μg of p458 (Konen Waisman et al., 1999). All peptides were emulsified in incomplete Freund's adjuvant (IFA), and injected at 50 μl/mouse intrafootpad (IFP). Mice were immunized with the different peptides 2-3 times according to the experimental protocol. One week after the last immunization, mice were bled and sacrificed. Spleens were harvested, splenocyte cultures prepared and tested for T cell proliferation and cytokine secretion. Blood samples were centrifuged (4000 rpm for 7 min), and then sera were collected and tested for anti-WNV antibodies by ELISA and neutralization assays.
To study the immunogenicity and protective efficacy of the peptides, groups of 3-week old mice were immunized and boosted according to the following protocol: mice were immunized IFP and boosted once or twice, 1 week apart. One week after the last boost, the mice were challenged IP with 1×106 pfu of WNV. Mortality was recorded for the next 21 days. For virology studies, surviving and moribund mice were sacrificed on day 7 after the challenge, and organs were harvested for virus titrations and RT-PCR.
Virus Load in Brain Tissue of Infected Mice
Brain tissues were removed from infected or immunized and challenged mice, and 10% (wt/vol) homogenates were prepared in DMEM-10% DMSO. The homogenates were then aliquoted and stored at −70° C. until further analysis. Virus levels were determined by plaque titration on Vero cell monolayers as previously described (Ben-Nathan et al., 1996), and expressed as pfu/0.1 gr brain tissue.
RNA Extraction and RT-PCR
RNA was extracted from brain tissues from mice using RNEasy Midi Kit (QIAGEN, Hilden, Germany), and RT-PCR was performed on RNA extracts using Endo-Free Reverse Transcriptase (Ambion, Huntingdon, UK) and Biomix-Red (Bioline, London, UK). WNV E protein primers (5′-ACGAAGTGGCCATTTTTGTC-3′, SEQ ID NO:81/5′-TTGATGCAGAGCTCCCTCTT-3′, SEQ ID NO:82) were chosen using Primer3 program (Whitehead Institute for Biomedical Research). PCR products were electrophoretically separated on 1.5% agarose gel, stained with ethidium bromide and imaged using a CCD camera (Imagechem 5500).
Preparation of Spleen Cell Cultures and Cytokine Analysis
Spleens of immunized mice were harvested, and spleen pulp was extruded from the capsule in RPMI-1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 10 mM HEPES and 5% FCS (base-RPMI). Spleen cell suspensions were passed through a cell strainer, washed, treated for 2 min with ACK lysing buffer (0.15M NH4Cl, 0.01 KHCO3) for elimination of erythrocytes, and washed twice in base-medium. Splenocytes were resuspended in RPMI-1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 10 mM HEPES, 5×10−5M β-mercaptoethanol and 10% FCS (complete RPMI) in a final concentration of 5×106 cells/ml in a 24-well plates. Cell suspensions were stimulated in vitro with either 10 μg/ml of different peptides, 10 μl/ml of WNV-Ag, or 5 μg/ml of Con-A. The cells were then incubated at 37° C. in 5% CO2 for 72 h. Supernatants were collected, diluted by 2, and IFNγ and IL-4 concentrations were measured by indirect ELISA method using commercial kits (PharMingen, San Diego, Calif.) according to manufacturer instructions.
Virus Plaque Reduction Neutralization Testing (PRNT)
The titer of neutralizing antibodies was determined using a modified plaque reduction test (PRNT). Briefly, serial 2 fold dilutions (1:4 to 1:512) of mouse sera were prepared in 96-well flat-bottom microtiter plates and 104 pfu of WNV in equal volumes was added to duplicate wells of each dilution. After 30 min of incubation at room temperature, 5×104 of Vero cells were added to each well of the sera-virus mixtures. The plates were incubated for 72 h in a humidified atmosphere at 37° C. and 5% CO2 and plaques were counted. Plaque-reduction neutralizing antibody titers were expressed as the reciprocal of the highest dilution that gave 50% plaque reduction (PRNT50).
Isotypes of Anti-WNV Antibodies
Plates were coated with the WNV-Ag diluted 1:700 in coating buffer. After overnight incubation at 4° C., blocking and washing, various dilutions of mouse sera were added in triplicates. Negative and positive controls of mouse sera were tested in each plate. Total IgG assessment was performed using HRP-Streptavidin-conjugated anti-mouse IgG (SouthernBiotech). For antibody isotype identification, incubation with biotin-conjugated anti-mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM antibodies (PharMingen, San Diego, Calif.) was followed by incubation with 1:1000 diluted HRP-Streptavidin (Jackson Laboratories, West Grove, Pa.). After substrate addition the color intensity was measured by ELISA-reader at 405 nm.
Based on the antigenic propensity method (Kolaskar et al., 1990), calculated using a free B-cell epitope program (BcePred Server, http://www.imtech.res.in/raghava/bcepred/index.html), and based on a free MHC epitope program ProPred server, http://www.imtech.res.in/raghava/propred), we have identified a p15 peptide from different WNV-E proteins (from the immunogenic E3 domain, aa LVTVNPFVSVATANS or aa LVTVNPFVSVATANA; SEQ ID NOS:11 and 12, respectively) as a candidate B-cell continuous epitope and MHC-II-restricted epitope of the WNV (recognized by different human and murine MHC-II molecules).
Next, it was examined whether the candidate epitope is recognized by a serum immunoglobulin pool taken from Israeli donors (IVIG-IL), which contains anti-WNV Abs. Wells were coated with the different peptides (1 μg/well) or with the WNV-Ag (1:700 dilution). After blocking and washing, IVIG-IL was added at 1:40 dilution and binding was detected. The results demonstrate for the first time that the p15 peptide is recognized by IVIG-IL (
We showed that HSP60-p458 (NEDQKIGIEIIKRALKI SEQ ID NO:2) used as a carrier peptide for a MHC-I epitope of CMV enhanced immune response to CMV and immunization with the chimeric peptide, p458-CMV epitope, cleared MCMV from salivary glands of mice challenged with the virus. In accordance we tested the WNV-protective efficacy of p458-p15 (NEDQKIGIEIIKRALKILVTVNPFVSVATANS) chimeric peptide (termed as p32, SEQ ID NO:13). Table 2 summarizes 5 different experiments; immunization with p15 alone showed slight protective efficacy while immunization with a mixture of p15 and p458 did not protect mice. Nonetheless, when mice were immunized with p32, the chimeric p458-p15 peptide, a high degree of protection against a fatal challenge with virulent WNV was achieved.
Mice were immunized 3 times with the different peptides or a peptide mix at 7 day intervals. Seven days after the 3rd immunization mice were challenged with 106 pfu of WNV and survival was monitored for 21 days post challenge. Results are the summary of 5 different experiments, performed in the same conditions.
To investigate whether immunization with p32 resulted in clearance of WNV, virus levels in the brain, which is the prime target organ of this neurotropic virus, were examined.
Mice were immunized 3 times with p32 at 7 day intervals. Seven days after the last immunization, immunized and non-immunized mice were challenged IP with 106 pfu of WNV. Seven days after challenge, mice were sacrifice and different organs were extracted (brain, lungs, heart, liver and spleen). Organs were taken also from control naïve mice (non-immunized and non-infected matched mice). Viral loads were determined by RT-PCR for viral genome and plaque assay for infectious virus titers (pfu/0.1 gr tissue). RT-PCR results are representative from one experimental mouse while infectious titers are the average for all experimental groups. * Detection level of the plaque assay is <101.
Virus levels were determined in the brain by RT-PCR and plaque assay on day 7 after challenge (
We next investigated the immune responses elicited following immunization with p32. Immunization with p32 induced high titer of WNV-specific Abs as compared to immunization with p15 alone (
Mice were either immunized 3 times with p32 or challenged with WNV. Seven days after the 3rd immunization or WNV challenge, respectively, mice were bled and titers of WNV-neutralizing Abs in sera were tested (PRNT50). Results are the average of 4 independent ELISA experiments.
Next, it was examined whether p32 immunization and sublethal infection with WNV induce similar isotypes of anti-WNV antibodies. WNV-Ag was used as the antigen in the ELISA plates.
WNV-specific T-cell responses were examined as follows: Six-week old BALB/c mice were infected with a sublethal dose of WNV (0.66×102 pfu), and spleens were harvested 6 days later. Spleen cell suspensions were prepared, cultured in 96 wells plates and incubated for 3 days with the 10 μg/ml of EP15, p32, p458 or Con-A (5 μg/ml) for 3 days. Cell proliferation was measured by the WST-1 method and the results are the average of 3 independent experiments. As shown in
Conjugates of p458 and Ec27 with the novel viral antigens of the invention, having amino acid sequences as set forth in SEQ ID NOS:13-14, 23, 56-75 and 77-79 (see Table 4 below), are synthesized as described above.
Mice are immunized 3 times with the different peptides or with control peptides (corresponding non-conjugated viral antigens and with the carrier peptides alone) at 7 day intervals, as described above. Seven days after the 3rd immunization mice are challenged with 106 pfu of the corresponding flavivirus and survival is monitored for 21 days post challenge.
In other experiments, the immunogenicity of the conjugates is determined by the following assays: in some experiments, mice are immunized and assayed for serum titers of virus-specific and viral peptide-specific antibodies by ELISA essays, as described above. In other experiments, LNC from immunized mice are examined in the presence of the corresponding viral antigen in proliferation assays and IFN-γ secretion assays, as described above.
Mice were immunized with the following peptides as described in Example 8 using the following peptides: p15 (SEQ ID NO:11), p32 (SEQ ID NO:13), Ec27-p15 (KKARVEDALHATRAAVEEGVLVTVNPFVSVATANS, SEQ ID NO:77), and Mock-p15 (EGDEATGANIKVALEALVTVNPFVSVATANS, SEQ ID NO:80—the p431 peptide fused to p15).
Spleens were harvested 10 days after the immunization and splenocytes were cultured with p15 (25 μg/ml) for 3 days. Cell proliferation and IFNγ levels in the supernatants of spleen cell cultures were measured. Results are the average of 3 independent proliferation experiments. Ec27-Ep15* and Ec27-Ep15** are two identical but independent groups in the same experiment.
As shown in
Sera from WNV-exposed and non-exposed human subjects was assayed for antibodies recognizing the novel WNV peptide antigens, p15 (SEQ ID NO:11) and p17 (SEQ ID NO:21).
Sera S2 and S6 are from human that were not infected with WNV while sera S1, S3-S5 and S7 are from human that had history of WNV infection. IVIG is pool of plasmas from Israeli citizens that was shown to contain antibodies against WNV.
As can be seen, both p15 and p17 were specifically recognized by WNV-exposed sera and not by sera from unexposed subjects. Human sera from Dengue-infected humans also did not recognize the p15 (data not shown). Thus, p15 and p17 are suitable diagnostic peptides useful for determining WNV exposure or infection.
To find dominant T helper cell epitopes derived from the sequence of the E. coli variant of HSP60 (GroEL), BALB/c mice were inoculated with heat-inactivated E. coli bacteria and the proliferative responses of draining LNCs to a set of overlapping GroEL peptides (Table 5), and the whole GroEL molecule (Purchased from Sigma), were analyzed.
BALB/c mice were immunized s.c. with 107 glutaraldehyde-attenuated E. coli bacteria (
T-cell proliferation assays were performed as follows: Draining lymph node cells (LNC) of immunized mice were cultured (2×105/well) in 200 μl RPMI 1640 medium supplemented with 2 mM glutamine, non-essential amino acids, 1 mM sodium pyruvate, 100 U/ml penicillin, 100 μg/ml streptomycin (BioLab, Jerusalem, Israel), 5×10−5M β-mercaptoethanol (Fluka AG, Buchs, Switzerland), 10 mM HEPES buffer (Sigma), and 1% syngeneic normal mouse serum. After four days of incubation, [3H]-thymidine (0.5 μCi of 5 Ci/mmol, Nuclear Research Center, Negev, Israel) was added for additional sixteen hours, and the thymidine incorporation was measured. Results are expressed as the mean cpm, or the stimulation index (SI), i.e. the mean cpm of test cultures (with antigen) divided by the mean cpm of control cultures (without antigen).
As can be seen in
It was also tested whether the Ec27 peptide itself is immunogenic by immunization of BALB/c mice with 20 μg of the Ec27 peptide in IFA.
BALB/c mice were immunized s.c. with 20 μg Ec27 peptide emulsified in IFA. Ten days later lymph node cells were taken and assessed for specific proliferation of 2×105 cells in the presence of the Ec27 peptide, the acetylcholine receptor peptide 259-271 (AcR 259-271, VIVELIPSTSSAV SEQ ID NO:84), or GroEL at the concentrations 10 μg/ml, 2 μg/ml, or without antigen (BG). Results are shown as mean cpm of quadruplicate wells. The standard deviations are indicated.
To learn whether the immunogenicity of the Ec27 peptide is restricted to a certain MHC haplotype, we compared four strains mice with different MHC haplotypes in their response to the Ec27 peptide. The immunized mice were of the BALB/c (H-2d), BALB/k (H-2k), BALB/b (H-2b), or SJL (H-2S) strain. As can be seen in
Because the Ec27 peptide was found to be an immunodominant peptide of the GroEL molecule, which is the major immunogen of bacteria, it was interesting to learn whether the Ec27 peptide can be used as an adjuvant, as can the bacteria. Therefore, BALB/c and C57BL/6 mice were immunized subcutaneously with 20 μg of the monoclonal anti-p53 antibody PAb-246 (described in Yewdell et al., 1986) in different immunogenic compositions: the antibody was either emulsified in Complete Freund's Adjuvant (246 in CFA), in Incomplete Freund's Adjuvant (246 in IFA), or together with 50 μg the Ec27 peptide (as a mixture) in IFA (246 in Ec27). Three weeks later, all mice received a boost of the PAb-246 antibody subcutaneously in IFA. Ten days after the boost, mice were bled and their antibody responses to the PAb-246 immunogen were compared by ELISA (
Dots represent individual sera, bars indicate the median of each group.
S. pneumoniae serotype 4 capsular polysaccharide (PnTy4) was obtained from the American Type Culture Collection (ATCC; Rockville, Md., USA). PnTy4 was coupled to carrier peptides by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (CDI; Aldrich, Wis., USA) using standard procedure.
The peptide carriers used in this example are: p458 (SEQ ID NO:2), p458s (SEQ ID NO:87, EGDIETGVNIVLKALTA), MOG (SEQ ID NO: 85, MEVGWYRSPFSRVVHLYRNGK).
BALB/c mice were immunized with the indicated peptide-polysaccharide conjugate in IFA or IFA mixed with 50 μg Ec27 as described above, in the WNV vaccination example. LNC were collected and assayed for nitric oxide (NO) production and proliferation.
Table 6 shows the levels of Nitric Oxide (NO) Production (nM) in lymph node cells (LNC) derived from mice immunized with peptide-sugar conjugates in IFA or in IFA+Ec27. The cells were cultured for 72 h with either Concanavalin A (Con A) or the peptide-sugar immunogen in the presence or absence of a macrophage cell line (J774). NO production was assayed using a Nitrate/Nitrile colorimetric assay according to the manufacturer's instructions.
The proliferation of lymph node cells in response to immunogen was performed as described above (Example 14).
Table 7 shows the proliferation of LNC derived from mice immunized with peptide-sugar conjugates in IFA or in IFA+ec27. The cells were cultured for 72 h with Concanavalin A (Con A), the peptide-sugar immunogen (Ty4), the peptide immunogen without the sugar, or the sugar conjugated to a control peptide (MOG-Ty4). The proliferative response is given as the stimulation index (SI).
In the tables, BG refers to background values; ConA refers to concanavalin A; P458-Ty4 is a conjugate between the p458 peptide and the S. pneumoniae serotype 4 capsular polysaccharide; P458s-Ty4 is a conjugate between the p458s peptide and the S. pneumoniae serotype 4 capsular polysaccharide.
This application is a divisional of U.S. patent application Ser. No. 11/908,474, filed May 15, 2008, which is U.S. national stage of PCT/IL2006/000222, filed Feb. 21, 2006, which is based on and claims the benefit of U.S. Provisional Patent Application No. 60/661,017, filed Mar. 14, 2005, the contents of each of which is expressly incorporated herein in its entirety by this reference.
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| 4073943 | Wretlind | Feb 1978 | A |
| 4168308 | Wretlind et al. | Sep 1979 | A |
| 5154923 | Van Eden | Oct 1992 | A |
| 5736146 | Cohen | Apr 1998 | A |
| 5869058 | Cohen et al. | Feb 1999 | A |
| 5961970 | Lowell | Oct 1999 | A |
| Number | Date | Country |
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| 262710 | Jun 1988 | EP |
| 9531994 | Nov 1995 | WO |
| 9010449 | Sep 1999 | WO |
| 02062959 | Aug 2002 | WO |
| 03026579 | Apr 2003 | WO |
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| Number | Date | Country | |
|---|---|---|---|
| 20110256165 A1 | Oct 2011 | US |
| Number | Date | Country | |
|---|---|---|---|
| 60661017 | Mar 2005 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 11908474 | US | |
| Child | 13164411 | US |
