Claims
- 1. Method for determining an analyte in a sample, comprising contacting said sample with a composition which comprises:
- (i) an analyte oxidizing agent,
- (ii) an electron transfer agent,
- (iii) a complex of ferric ions and a chelating agent selected from the group consisting of a citrate ion containing compound, a malate ion containing compound having an affinity for ferric ions greater than its affinity for ferrous ions, with the proviso that an iminodiacetate containing compound is not present, and
- (iv) a ferrous ion complexing agent which forms a color when complexed to ferrous ions, and determining formation of a color in said sample as a determination of said analyte therein.
- 2. The method of claim 1, wherein said analyte oxidizing agent comprises at least one enzyme.
- 3. The method of claim 2, wherein said at least one enzyme is glucose oxidase.
- 4. The method of claim 2, wherein said at least one enzyme is cholesterol oxidase.
- 5. The method of claim 1, wherein said electron transfer agent is a ferricyanide.
- 6. The method of claim 1, wherein said electron transfer agent is phenazine ethosulfate.
- 7. The method of claim 1, wherein said electron transfer agent is phenazine methosulfate.
- 8. The method of claim 1, wherein said chelating agent is a citrate ion containing a compound.
- 9. The method of claim 1, wherein said chelating agent is a malate ion containing compound.
- 10. The method of claim 1, wherein said ferrous ion complexing agent is ferrozine.
- 11. The method of claim 1, wherein said composition further comprises a buffer.
Parent Case Info
This application is a divisional of Ser. No. 08/010,232 filed Jan. 28, 1993, now abandoned.
US Referenced Citations (5)
Non-Patent Literature Citations (1)
Entry |
Al-Jobore et al; Chem. Abstract 94(1): 1872p from Can. J. Biochem., 58(12), 1397-1404 (1980). |
Divisions (1)
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Number |
Date |
Country |
Parent |
10232 |
Jan 1993 |
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