Patent Application
20210338690
The present invention provides some anti-coronaviral compounds and the method and composition/pharmaceutical composition for prevention and treatment of coronavirus infections, particularly the disease caused by SARS-COV-2.
Severe acute respiratory syndrome (SARS) outbreak in Nov. 1, 2002 to Jun. 18, 2003 led to 801 deaths in over 29 countries and 8465 probable cases around the world according to the World Health Organization (WHO) (Chen et al., 2005). SARS, an enveloped β coronavirus containing positive-sense, single-stranded RNA, has a genome size of about 30 kb, in which open reading frame (ORF) 1a and 1b encode for two respective polyproteins (pps), pp1a and pp1ab (Hegyi et al., 2002; Needle et al., 2015). To complete its lifecycle, successful replication and proteolytic processing are imperative (Herold et al., 1998). Indeed, the consensus functions of these virus-encoded proteolytic proteins are found in all coronaviruses, specifically papline-like protease (PLpro) and chymotrypsin-like protease (3CLpro) (Herold et al., 1998). In proteolytic processing of pp1a and pp1ab, PLpro and 3CLpro cleave the first three sites and the remaining 11 locations, respectively, yielding a total of 16 nonstructural proteins (nsp1-16) (Hegyi et al., 2002; Needle et al., 2015). Thus, 3CLpro inhibition has been regarded as a molecular approach in anti-SARS drug discovery and developments (Chen et al., 2005; Jo et al., 2020).
SARS-COV-2 is a novel coronavirus that spreads rapidly since its identification in patients with severe pneumonia in Wuhan, China (named as COVID-19), has been reported in 25 countries, with nearly 72000 laboratory-confirmed cases and a death toll of 1775 worldwide as of Feb. 17, 2020 (Coronavirus disease 2019 (Covid-19) Situation Report—28, 2020; Li & De Clercq, 2020). Devastatingly, no drug or vaccine has yet been approved to treat human coronaviruses (Li & De Clercq, 2020). Concerning the current outbreak of SARS-CoV-2 and the therapeutic experience of SARS and MERS (another β coronavirus), many studies extensively investigate the possibility of using the existing antiviral agents used for HIV, hepatitis B virus, hepatitis C virus and influenza infections for the treatment or intervention of SARS-COV-2 (De Clercq & Li, 2016; Li & De Clercq, 2020). In the meantime, SARS-CoV-2 has been characterized as an enveloped, positive-sense, single-stranded RNA β coronavirus, similar to SARS and MERS (Li & De Clercq, 2020). Consistent with the characteristics of coronaviruses, SARS-CoV-2 genome encodes structural proteins (e.g., spike glycoproteins), nonstructural proteins (e.g., 3CLpro, PLpro, helicase, RNA-dependent RNA polymerase), and accessory proteins. Regarding the available genomic sequence of SARS-COV-2, SARS and MERS, a high-level conservation of the proteolytic sites and proteolytic enzymes was found, whence repurposing SARS and MERS protease inhibitors for treatment of SARS-COV-2 is worth considering (Liu et al., 2020). As 3CLpro plays a pivotal role in SARS, it is reasonable to approach protease inhibition by targeting the 3CLpro of SARS-COV-2 instead of its PLpro to intercept its lifecycle (Chen et al., 2005; Jo et al., 2020; Liu et al., 2020).
Currently, disulfiram, an approved drug to treat alcohol dependence, has been reported to inhibit the PLpro of MERS and SARS in cell cultures but has yet been evaluated clinically (Li & De Clercq, 2020). In addition, clinical trials of HIV protease inhibitors (lopinavir and ritonavir) in SARS-CoV-2 patients have also commenced, yet it is uncertain if it can effectually inhibit those of SARS-CoV-2, as HIV and β coronavirus proteases belong to the aspartic protease family and the cysteine protease family, respectively (Li & De Clercq, 2020; Zumla et al., 2016). On the other hand, remdesivir, a nucleotide analog of RNA dependent RNA polymerase inhibitor approved for HIV treatment, is currently under clinical trials in SARS-CoV-2 patients with estimated completion dates in April, 2020; galidesivir, another nucleotide analog of RNA dependent RNA polymerase inhibitor in early-stage clinical studies for HCV treatment, has shown broad-spectrum antiviral activities against severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS) in preclinical studies (Wang et al., 2020; Zumla et al., 2016). However, one might expect that a nucleoside analog can elicit toxicity that are still beyond our knowledge (Feng, 2018).
There are yet to find vaccines or antiviral drugs to prevent or treat human coronavirus infections. There is an urgent need for exploring and developing a safe anti-coronavirus therapy, particularly against SARS-COV-2.
It is unexpectedly found in the present invention that some triterpenes are effective in inhibition of coronavirus infections, especially SARS-COV-2.
In one object, the present invention provides a method for preventing and/or treating a coronavirus infection, comprising administering to a subject in need thereof a compound or a pharmaceutically acceptable salt thereof, in which the compound is selected from the group consisting of:
wherein R1 is O, α-OH or β-H; R2 is H or OH; R3 is O, α-H, β-OH, β-OAc or H2; R4 is H or OH; R5 is H, OH or ORx; R6 is COORx or COO(CH2)n—CH3; n is an integer from 0-3; R7 is H, Oh, ORx or OAc; R8 is CH3 or COORx; R21 is CH3, COORx, or COO(CH2)n—CH3; n is an integer from 0-3; the dotted line represents a single bond or a double bond, Rx is H or a C1-8 alkyl; and
wherein each of R21, R22, R23, R24, R25, R26, R27, R28, R29, R30, X1 and X2 is H, OH, C1-8 alkyl ,NRx, SRx, ORx, pyrazoline, cysteine, glutathione, halogen, COORx, or COO(CH2)n—CH3; n is an integer from 0-3; each of Y1, Y2, Z1, Z2, W1 and W2 is H, OH, C1-8 alkyl, or X1 and X2together form —O—, or Y1 and Y2, Z1 and Z2, or W1 and W2 together form an epoxy.
In some particular examples of the present invention, the compound is selected from the group consisting of:
In one further aspect, the present invention provides a composition/pharmaceutical composition for preventing and/or treating a coronavirus infection, particularly SARS-COV-2 comprising a therapeutically effective amount of any of the compounds disclosed herein or pharmaceutically acceptable thereof, or its mixture, in combination of a pharmaceutically acceptable carrier.
Optionally, the composition/pharmaceutical composition according to the invention may comprise at least one additional anti-viral therapeutic agent.
In one yet aspect, the present invention provides a use of any of the compounds disclosed herein or pharmaceutically acceptable salts, or its mixture for manufacturing a medicament for preventing and/or treating a coronavirus infection, particularly SARS-COV-2.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention.
The foregoing summary, as well as the following detailed description of the invention, will be better understood when read in conjunction with the appended drawings. For the purpose of illustrating the invention, there are shown in the drawings embodiments which are presently preferred.
In the drawings:
The above summary of the present invention will be further described with reference to the embodiments of the following examples. However, it should not be understood that the content of the present invention is only limited to the following embodiments, and all the inventions based on the above-mentioned contents of the present invention belong to the scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by a person skilled in the art to which this invention belongs.
As used herein, the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a sample” includes a plurality of such samples and equivalents thereof known to those skilled in the art.
In the present invention, to evaluate the effect of prospecting drugs on proteolytic processing inhibition in high-throughput, the synthetic peptides labelled fluorescence resonance energy transfer (FRET) pairs were employed as those used in the previous studies, in which the quenched fluorophore is released upon cleavage of the FRET-labelled peptides, generating fluorescent signals that can be monitored in real-time (Chen et al., 2005; Jean et al., 1995; Jo et al., 2020). It is confirmed in the present invention that any or its mixture of the compounds disclosed herein is effective in inhibition of a cysteine protease, particularly 3CLpro of SARS-CoV-2.
The present invention provides a method for preventing and/or treating a coronavirus infection, particularly SARS-COV-2 comprising administering to a subject in need thereof a compound or pharmaceutically acceptable salt thereof, or its mixture, in which the compound is selected from the group consisting of:
wherein R1 is O, α-OH or β-H; R2 is H or OH; R3 is O, α-H, β-OH, β-OAc or H2; R4 is H or OH; R5 is H, OH or ORx; R6 is COORx or COO(CH2)n—CH3; n is an integer from 0-3; R7 is H, Oh, ORx or OAc; R8 is CH3 or COORx; R21 is CH3, COORx, or COO(CH2)n—CH3; n is an integer from 0-3; the dotted line represents a single bond or a double bond, Rx is H or a C1-8 alkyl; and
wherein each of R21, R22, R23, R24, R25, R26, R27, R28, R29, R30, X1 and X2 is H, OH, C1-8 alkyl, NRx, SRx, ORx, pyrazoline, cysteine, glutathione, halogen, COORx, or COO(CH2)n—CH3; n is an integer from 0-3; each of Y1, Y2, Z1, Z2, W1 and W2 is H, OH, C1-8 alkyl, or X1 and X2 together form —O—, or Y1 and Y2, Z1 and Z2, or W1 and W2 together form an epoxy.
The present invention also provides a composition/pharmaceutical composition for preventing and/or treating a coronavirus infection, particularly SARS-COV-2 infection, which comprises a therapeutically effective amount of a compound as disclosed herein or mixture thereof and a pharmaceutically acceptable carrier.
In one embodiment, the compound is
wherein R1 is O, α-OH or β-H; R2 is H or OH; R3 is O, α-H, β-OH, β-OAc or H2; R4 is H or OH; R5 is H, OH or ORx; R6 is COORx or COO(CH2)n—CH3; n is an integer from 0-3; R7 is H, Oh, ORx or OAc; R8 is CH3 or COORx; R21 is CH3, COORx, or COO(CH2)n—CH3; n is an integer from 0-3; the dotted line represents a single bond or a double bond, Rx is H or a C1-8 alkyl; or
wherein each of R21, R22, R23, R24, R25, R26, R27, R28, R29, R30, X1 and X2 is H, OH, C1-8 alkyl, NRx, SRx, ORx, pyrazoline, cysteine, glutathione, halogen, COORx, or COO(CH2)n—CH3; n is an integer from 0-3, Rx is H or a C1-8 alkyl; each of Y1, Y2, Z1, Z2, W1 and W2 is H, OH, C1-8 alkyl, X1 and X2 together form —O—, or Y1 and Y2, Z1 and Z2, or W1 and W2 together form an epoxy.
In the example of the invention, the compound of formula (I) may be:
In another example of the invention, the compound of formula (I) may be:
In one yet example of the invention, the compound of formula (I) may be:
In further example of the invention, the compound of formula (I) may be:
In one particular example of the invention, the compound of formula (I) may be lanostane:
In addition, the compound of formula (II) may be
or its derivatives, such as
Accordingly, the preferred compound is selected from the group consisting of:
According to the invention, the most preferred compound is selected from the group consisting of:
In one particular example of the invention, the compound of formula (II) is
The term “coronavirus” as used herein refers to a Coronaviruse in the subfamily Orthocoronavirinae, the family Coronaviridae, order Nidovirales, and realm Riboviria, which is enveloped viruses with a positive-sense single-stranded RNA genome and a nucleocapside of helical symmetry. They have characteristic club-shaped spikes that project from their surface, which in electron micrographs create an image reminiscent of the solar corona from which their name derives. Coronaviruses cause diseases in mammals and birds, including humans. In humans, coronaviruses cause respiratory tract infections, including common cold, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and SARS-COV-2.
The term “cysteine protease” as used herein refers to thiol proteases, are enzymes that degrade proteins, sharing a common catalytic mechanism that involves a nucleophilic cysteine thiol in a catalytic triad or duad. One example of cysteine protease in a virus is 3CLpro in SARS-COV-2.
The term “treat,” “treating” or “treatment” as used herein refers to the application or administration of a composition including one or more active agents to a subject afflicted with a disease, a symptom or conditions of the disease, or a progression of the disease, with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improve, or affect the disease, the symptoms or conditions of the disease, the disabilities induced by the disease, or the progression of the disease.
The term “prevent,” “prevention” or “preventing” as used herein refers to the prevention of the recurrence, onset, or development of a virus infection, one or more symptoms thereof, or a respiratory condition associated with, potentiated by, or potentiating a coronavirus infection in a subject.
The term “subject” as used herein includes human or non-human animals, such as companion animals (e.g. dogs, cats, etc.), farm animals (e.g. cattle, sheep, pigs, horses, etc.), or experimental animals (e.g. rats, mice, guinea pigs, etc.).
The term “therapeutically effective amount” as used herein refers to an amount of a pharmaceutical agent which, as compared to a corresponding subject who has not received such amount, results in an effect in treatment, healing, prevention, or amelioration of a disease, disorder, or side effect, or a decrease in the rate of advancement of a disease or disorder. The term also includes within its scope amounts effective to enhance normal physiological function.
For use in therapy, the therapeutically effective amount of the compound is formulated as a pharmaceutical composition for administration. Accordingly, the invention further provides a pharmaceutical composition comprising a therapeutically effective amount of any or its mixture of these compounds disclosed herein, and one or more pharmaceutically acceptable carriers.
For the purpose of delivery and absorption, a therapeutically effective amount of the active ingredient according to the present invention may be formulated into a pharmaceutical composition in a suitable form with a pharmaceutically acceptable carrier. Based on the routes of administration, the pharmaceutical composition of the present invention comprises preferably from 0.1% to 100% in weight of the total weight of the active ingredient.
The term “pharmaceutically acceptable carrier” used herein refers to a carrier(s), diluent(s) or excipient(s) that is acceptable, in the sense of being compatible with the other ingredients of the formulation and not deleterious to the subject to be administered with the pharmaceutical composition. Any carrier, diluent or excipient commonly known or used in the field may be used in the invention, depending to the requirements of the pharmaceutical formulation. Said carrier may be a diluent, vehicle, excipient, or matrix to the active ingredient. Some examples of appropriate excipients include lactose, dextrose, sucrose, sorbose, mannose, starch, Arabic gum, calcium phosphate, alginates, tragacanth gum, gelatin, calcium silicate, microcrystalline cellulose, polyvinyl pyrrolidone, cellulose, sterilized water, syrup, and methylcellulose. The composition may additionally comprise lubricants, such as talc, magnesium stearate, and mineral oil; wetting agents; emulsifying and suspending agents; preservatives, such as methyl and propyl hydroxybenzoates; sweeteners; and flavoring agents.
The composition of the present invention can provide the effect of rapid, continued, or delayed release of the active ingredient after administration to the patient. According to the invention, the pharmaceutical composition may be adapted for administration by any appropriate route, including but not limited to oral, rectal, nasal, topical, vaginal, or parenteral route (such as intramuscular, intravenous, subcutaneous, and intraperitoneal), transdermal, suppository, and intranasal methods.
Regarding parenteral administration, it is preferably used in the form of a sterile water solution, which may comprise other substances, such as salts or glucose sufficient to make the solution isotonic to blood. The water solution may be appropriately buffered (preferably with a pH value of 3 to 9) as needed. Preparation of an appropriate parenteral composition under sterile conditions may be accomplished with standard pharmacological techniques well known to persons skilled in the art.
In one particular example of the invention, the pharmaceutical composition is formulated for oral administration. Such formulations may be prepared by any method known in the art of pharmacy. According to the present invention, the form of said composition may be tablets, pills, powder, lozenges, packets, troches, elixers, suspensions, lotions, solutions, syrups, soft and hard gelatin capsules, suppositories, sterilized injection fluid, and packaged powder.
In the invention, the method and composition/pharmaceutical composition are effective in treating a virus infection through an inhibition of a cysteine protease in a virus, particularly an RNA-dependent virus. Accordingly, the invention also provides a method and composition/pharmaceutical composition for treatment and/or prevention of a virus infection through inhibition of a cysteine protease in a virus, comprising using the compounds disclosed herein or pharmaceutically acceptable salt thereof.
Exemplified viruses which are responsive include, without limitation, a coronavirus, and a HIV. Preferably, the viral infection is a coronavirus. More preferably, the viral infection is a SARS, MERS and SARS-COV-2.
In another aspect, the present invention provides a method for treating or preventing an RNA-dependent virus infection through inhibiting a cysteine protease in a virus. One example of the virus is an RNA-dependent virus, such as SARS, MERS and SARS-COV-2; particularly SARS-COV-2.
In one further aspect, the present invention provides a composition/pharmaceutical composition for treating and/or preventing a virus infection through inhibiting a cysteine protease in a virus, which comprises any of the compounds disclosed herein, pharmaceutically acceptable salt thereof, or its mixture. Optionally, the composition/pharmaceutical composition may comprise at least one additional anti-viral therapeutic agent.
In one further aspect, the present invention provides a use of any of the compounds disclosed herein for manufacturing a medicament for treating or preventing a virus infection through inhibiting a cysteine protease in a virus.
The present invention is further illustrated by the following examples, which are provided for the purpose of demonstration rather than limitation.
I. FRET Protease Assays with the SARS-CoV-2 3CLpro
The establishment of an ED-FRET platform follows the protocol given by Jo et al. (2020). Briefly, a custom proteolytic, fluorogenic peptide with DABCYL and EDANS on ends, DABCYL-TSAVLQSGFRKMG-EDANS (Genomics, Taiwan), contains the consensus nsp4/nsp5 cleavage sequence that can be recognized by 3CLpro of SARS-CoV-2. The peptide is dissolved in distilled water and incubated with 3CLpro of SARS-CoV-2. Measurements of the spectral-based fluorescence are determined by a SPARK® multimode microplate reader provided by TECAN. The proteolytic activity is determined at 37° C. by fluorescent intensity of EDANS upon peptide hydrolysis as a function of time, in which λexcitatron=340 nm, λemission=490 nm, bandwidths=9, 15 nm, respectively. Prior to the assay, the emission wavelength of the testing drugs at 340 nm excitation is examined to ensure that it does not overlap with the emission spectrum of EDANS.
Assays are conducted in triplicate in black 96-well microplates (Greiner) in 100 μL assay buffers containing 3CLpro of SARS-CoV-2 and the customized peptide. In SARS 3CLpro assay, 1 μM SARS-CoV-2 3CLpro containing 50 mM Tris pH 6.5 is incubated with 5 μM fluorescent substrate at 37° C. for 3 h before measuring Relative Fluorescence Unit (RFU).
II. Inhibition Assays in the Presence of the Compounds According to the Invention
There are five example compounds (AR100-DS1, AR101-DS1, AR101-DS2, AR101-DS3, and AR101-DS4) in the first screening process at 20 μM (n=3). The five compounds are given in the table below.
At first, the SARS-CoV-2 3CLpro and Arjil drugs will be mixed and pre-incubate at 37° C. for 1 h. Those manifesting inhibitory activity against 3CLpro of SARS-CoV-2 will be investigated further at different concentrations to characterize their IC50 values, using GraphPad Prism 7.03 (GraphPad Software, San Diego, Calif., USA).
Based the knowledge and sequence-based SARS-CoV-2 3CLpro, the efficacy of 3CLpro inhibiting the compounds according to the invention were assessed in vitro to determine their therapeutic potential in SARS-CoV-2 treatment. Concerning that no drug or vaccine has yet been approved to treat human SARS-CoV-2 infection, developing a broad-spectrum antiviral agent to combat against SARS-CoV-2 is of utmost importance and urgency. Enactment of ED-FRET technology and its workflow provides a robust, high-throughput drug discovery in the lab. Meanwhile, identification of SARS-CoV-2 3CLpro inhibiting agents from the 5 example compounds according to the invention acts as guidelines of probable therapeutic doses in clinical assessment and prompts patent application in the future, contributing to antiviral library construction.
III. Results
1. Identification of SARS-CoV-2 3CLpro Inhibitors
To determine the efficacy of the five example compounds according to the invention, SARS-CoV-2 3CLpro, IQF peptide substrate, and the example compounds were incubated in the constructed ED-FRET platform at 0.5 μM, 5 μM and 20 μM, respectively. As shown in
2. Characterization of the Half Maximal Inhibitory Concentration of Inhibitors
The inhibitory profiles of AR100-DS1, AR101 DS1, AR101 DS2, AR101 DS3 and AR101 DS4 at the concentration of 20 μM were determined and given in
The IC50 values of AR100 DS1 were given in
Given the above, all the example compounds including AR100 DS1, AR101 DS1, AR101 DS2, AR101 DS3 and AR101 DS4 were confirmed to have the inhibitory effect on SARS-CoV-2 3CLpro highlights their therapeutic potentials against SARS-CoV-2, particularly AR100 DS1, AR101 DS2 and AR101 DS3.
All publications, patents, and patent documents cited herein above are incorporated by reference herein, as though individually incorporated by reference.
The invention has been described with reference to various specific and preferred embodiments and techniques. However, one skilled in the art will understand that many variations and modifications may be made while remaining within the spirit and scope of the invention.
This application claims the benefit of prior U.S. Provisional Application Ser. No. 63/018,690 filed May 1, 2020, the disclosure of which is incorporated herein in it its entirety for all purposes.
| Number | Date | Country | |
|---|---|---|---|
| 63018690 | May 2020 | US |
