Patent Application
20210171563
The present invention is directed to various compounds, compositions, and methods for treating bacterial infections such as urinary tract infections.
Urinary tract infections (UTIs) are one of the most prevalent infections, afflicting 15 million women per year in the United States alone with annual healthcare costs exceeding $2-3 billion [1-3]. Nearly 50% of women will experience at least one UTI in their lifetime. Despite appropriate and often successful clearance of bacteriuria by antibiotic treatment, 20-30% of women will experience a recurrence within six months of the initial acute UTI [1,4]. Kidney infection, or pyelonephritis, represents a severe manifestation of UTI, with approximately 250,000 cases and 100,000 hospitalizations per year in the US [5]. Acute pyelonephritis requires hospital admission and intravenous antibiotics to thwart the long-term sequelae of kidney failure and renal scarring, and together with bacteremia, results in a mortality rate of 10-20% [6-8]. With the global dissemination and increase of antibiotic resistance, treatment of UTI is becoming a serious clinical challenge [9]. Antibiotic susceptibility tests indicate that many uropathogens are resistant to traditional first-line antibiotics like trimethoprim-sulfamethoxazole (TMP-SMZ) and even to last-line antibiotics such as ciprofloxacin and colistin [10-15]. The diminishing efficacy of antibiotic therapies toward UTIs and other infectious diseases necessitates alternative approaches to combatting bacterial pathogens. Recent efforts aim to target the virulence mechanisms that cause bacterial infection to provide much-needed therapeutic alternatives while simultaneously reducing the burden of antibiotic resistance and minimizing disruption of gastrointestinal microbial communities that are beneficial to human health [16].
Uropathogenic E. coli (UPEC) are the main etiological agent of UTIs, accounting for greater than 80% of community-acquired UTIs [17, 18]. Comparative genomic studies have revealed that UPEC are remarkably diverse such that only 60% of the genome is shared amongst all strains {Schreiber, 2017 #4724}. As a consequence, UTI risk and outcome are determined by complex interactions between host susceptibility and diverse bacterial urovirulence potentials, which can be driven by differences in the expression and regulation of conserved functions. The ability of UPEC to colonize various habitats such as gut, kidney and bladder, depends in large part on the repertoire of adhesins encoded in their genome. The most common mechanism for adhesion utilized by UPEC is mediated through the chaperone-usher pathway (CUP), which generates extracellular fibers termed pili that can confer bacterial adhesion to host and environmental surfaces, facilitate invasion into host tissues, and promote interaction with other bacteria to form biofilms [19]. Phylogenetic analysis of Escherichia genomes and plasmids predicts at least 38 distinct CUP pilus types, with single organisms capable of maintaining as many as 16 distinct CUP operons [20]. Many of these CUP pilus operons contain two-domain, tip-localized adhesins, each of which likely recognize specific ligands or receptors to mediate colonization of a host and/or environmental niche. For example, the type 1 pilus adhesin FimH binds mannosylated glycoproteins on the surface of the bladder epithelium, which is crucial for the establishment of cystitis [21, 22]. The structural basis of mannose recognition by the N-terminal receptor binding domain, or lectin domain (LD), of FimH has been leveraged to rationally develop high-affinity aryl mannosides [23-26]. In mouse models of UTI, it has been previously demonstrated that orally bioavailable mannosides that tightly bind FimH can prevent acute UTI, treat chronic UTI, and potentiate the efficacy of existing antibiotic treatments like TMP-SMZ, even against antibiotic-resistant E. coli strains [26]. Thus, use of mannosides that target the adhesin FimH represents the first successful application of an anti-virulence strategy in the treatment of UTI.
A homolog of the type 1 pilus, the F9 pilus is one of the most common CUP pili in the E. coli pan genome and an important urovirulence factor employed by UPEC for the maintenance of UTI {Wurpel, 2013 #4695; Wurpel, 2014 #4664}. It has been demonstrated that UPEC up-regulate the expression of F9 pili in response to bladder inflammation and epithelial remodeling induced upon UPEC infection [27]. These pili display the FimH-like adhesin FmlH, which is capable of binding terminal galactose (Gal), N-acetylgalactosamine (GalNAc), or Thomsen-Friedenreich (TF) antigen (Gal-β1-3-GalNAc-α). FmlH was shown to bind TF antigen within naive or infected kidneys and GalNAc or Tn antigen receptors within the inflamed bladder epithelium during chronic, unresolved UTI. Deletion of FmlH in the urosepsis isolate CFT073 resulted in a competitive defect in the ability of this strain to maintain murine UTI in C3H/HeN female mice. Furthermore, vaccination with the lectin domain of FmlH (FmlHLD) as the challenge antigen significantly protected mice from developing UTI. Thus, FmlH has been shown to serve a key role in the UPEC pathogenesis cascade and represents a promising new target for novel anti-virulence therapies for UTI in both the bladder and kidney habitats.
Generally, the present invention relates to various compounds, compositions, and methods that are useful for treating bacterial infections, including urinary tract infections. In various aspects, the present invention is directed to compounds of Formula (I) or salts or prodrugs thereof:
wherein:
each X is independently hydrogen, fluoro, or OR2;
each R2 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Y is O, S, substituted or unsubstituted hydrocarbylene, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted thio, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2,
each R3 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Z is hydrogen, OR4, SR4, or NHR4;
each R4 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each m is independently an integer from 0 to 10; and
R1 is a substituent of Formula (II), (III), (IV), (V), (VI), (VII), (VIII), (IX), (IXa), (X), (XI), (XII), (XIII), (XIV), or (XV):
wherein:
each A is independently CR5 or N;
each B is independently O, S, C(O), C(R5)2, or NR6;
each R5 is independently hydrogen, oxygen, halo, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)pN(R7)2, (CH2)pSO2R7, (CH2)pN(SO2R7)2, (CH2)pNR7C(O)R7, (CH2)pNR7C(R7)2OR7, (CH2)pNR7CO2R7, (CH2)pNR7C(O)N(R7)2, (CH2)pNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)pN(R7)SO2N(R7)2, or (CH2)pOSO2R7;
each R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)qNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)qN(R7)2, (CH2)pN(SO2R7)2, (CH2)pSO2R7, (CH2)qNR7C(O)R7, (CH2)qNR7C(R7)2OR7, (CH2)qNR7CO2R7, (CH2)qNR7C(O)N(R7)2, (CH2)qNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)qN(R7) SO2N(R7)2, or (CH2)pOSO2R7;
each R7 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each n is independently an integer from 0 to 10;
each p is independently an integer from 0 to 10; and
each q is independently an integer from 1 to 10, with the proviso that:
(a) when (i) each X is OH, (ii) Y is O, (iii) Z is OR4, (iv) R4 is hydrogen, (v) R1 is a substituent of Formula (II), and (vi) n is 0, then R1 is not unsubstituted phenyl and each R5 is not nitro or amino;
(b) when (i) each X is OH, (ii) Y is O, (iii) Z is OR4, (iv) R4 is hydrogen, (v) R1 is a substituent of Formula (VII), and (v) B is N, then R1 is not unsubstituted indolyl and each R5 is not chlorine;
c) when (i) each X is OH, (ii) Y is O, (iii) Z is OR4, (iv) R4 is hydrogen, and (v) R1 is a substituent of Formula (IX), then R1 is not unsubstituted biphenyl;
(d) when (i) each X is OH, (ii) Y is O, (iii) Z is OR4, (iv) R4 is hydrogen, and (v) R1 is a substituent of Formula (X), then R1 is not
(e) when (i) each X is OH, (ii) Y is O, (iii) Z is OR4, (iv) R4 is hydrogen, and (v) R1 is a substituent of Formula (XI) or (XII), then each B is not C(O);
(f) when (i) each X is OH, (ii) Y is S, (iii) Z is OR4, (iv) R4 is hydrogen, and (v) R1 is a substituent of Formula (II), and (vi) n is 2, then R1 is not unsubstituted phenyl; and
(g) when (i) each X is OH, (ii) Y is O, (iii) Z is NHC(O)CH3, (iv) R1 is a substituent of Formula (II), and (v) n is 0, then R1 is not unsubstituted phenyl and each R5 is not nitro.
The present invention further relates to pharmaceutical compositions comprising a therapeutically effective amount of at least one compound described by Formula (I) or salt or prodrug thereof:
wherein:
each X is independently hydrogen, fluoro, or OR2;
each R2 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Y is O, S, substituted or unsubstituted hydrocarbylene, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted thio, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2,
each R3 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Z is hydrogen, OR4, SR4, or NHR4;
each R4 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each m is independently an integer from 0 to 10; and
R1 is a substituent of Formula (II), (III), (IV), (V), (VI), (VII), (VIII), (IX), (IXa), (X), (XI), (XII), (XIII), (XIV), or (XV):
wherein:
each A is independently CR5 or N;
each B is independently 0, S, C(O), C(R5)2, or NR6;
each R5 is independently hydrogen, oxygen, halo, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)pN(R7)2, (CH2)pSO2R7, (CH2)pN(SO2R7)2, (CH2)pNR7C(O)R7, (CH2)pNR7C(R7)2OR7, (CH2)pNR7CO2R7, (CH2)pNR7C(O)N(R7)2, (CH2)pNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)pN(R7)SO2N(R7)2, or (CH2)pOSO2R7;
each R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R′, (CH2)qN(R7)2, (CH2)pN(SO2R7)2, (CH2)pSO2R7, (CH2)qNR7C(O)R7, (CH2)qNR7C(R7)2OR7, (CH2)qNR7CO2R7, (CH2)qNR7C(O)N(R7)2, (CH2)qNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)qN(R7) SO2N(R7)2, or (CH2)pOSO2R7;
each R7 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each n is independently an integer from 0 to 10;
each p is independently an integer from 0 to 10; and
each q is independently an integer from 1 to 10.
The present invention also relates to methods of use including a method of treating a bacterial infection comprising administering to a subject in need thereof a pharmaceutical composition comprising a therapeutically effective amount of at least one compound as described herein.
Other objects and features will be in part apparent and in part pointed out hereinafter.
Panel shows a synthetic scheme for rationally designed compound 29β-NAc, with the carboxylic acid designed to interact with R142, the phenyl ring designed to interact with Y46, and the N-Acetyl group designed to interact with K132.
Corresponding reference characters indicate corresponding parts throughout the drawings.
Generally, the present invention is directed to various compounds, compositions, and methods for treating bacterial infections such as urinary tract infections (UTI).
Applicants have discovered various high-affinity galactosides (e.g., aryl galactosides and N-acetylgalactosaminosides) that bind to and inhibit FmlH, conferring significant therapeutic efficacy in the treatment of bacterial infections including chronic UTI. This discovery supports the mechanistic and therapeutic value of anti-virulence strategies that aim to define the structure-function relationships of diverse bacterial adhesins at the molecular level and to leverage these structural biology insights to high-affinity galactosides for the treatment of UTI and other bacterial infections.
In accordance with the present invention, various compounds useful for inhibiting FmlH include compounds of Formula (I) or salt or prodrug thereof:
wherein:
each X is independently hydrogen, fluoro, or OR2; each R2 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Y is O, S, substituted or unsubstituted hydrocarbylene, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted thio, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2,
each R3 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Z is hydrogen, OR4, SR4, or N(R4)2 (e.g., preferably NHR4);
each R4 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each m is independently an integer from 0 to 10; and
R1 is a substituent of Formula (II), (III), (IV), (V), (VI), (VII), (VIII), (IX), (IXa), (X), (XI), (XII), or (XIII):
wherein:
each A is independently CR5 or N;
each B is independently O, S, C(O), C(R5)2, or NR6;
each R5 is independently hydrogen, oxygen, halo, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)pN(R7)2, (CH2)pSO2R7, (CH2)pN(SO2R7)2, (CH2)pNR7C(O)R7, (CH2)pNR7C(R7)2OR7, (CH2)pNR7CO2R7, (CH2)pNR7C(O)N(R7)2, (CH2)pNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)pN(R7)SO2N(R7)2, or (CH2)pOSO2R′;
each R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)qN(R7)2, (CH2)qN(SO2R7)2, (CH2)pSO2R7, (CH2)qNR7C(O)R7, (CH2)qNR7C(R7)2OR7, (CH2)qNR7CO2R7, (CH2)qNR7C(O)N(R7)2, (CH2)qNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)pN(R7)SO2N(R7)2, or (CH2)pOSO2R7;
each R7 is independently hydrogen or substituted or unsubstituted hydrocarbyl,
each n is independently an integer from 0 to 10,
each p is independently an integer from 0 to 10;
each q is independently an integer from 1 to 10 with the proviso that:
In various embodiments, each X is independently OH. In various embodiments each X is independently OR2 and each R2 is independently hydrogen, substituted or unsubstituted C1-C30 alkyl. In some embodiments, R2 is independently hydrogen or C1-C30 alkyl.
In various embodiments, Y is O, S, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2. In some embodiments, m is an integer between 0 and 5. In these and other embodiments, each R3 is independently hydrogen or substituted or unsubstituted C1-C30 alkyl. In some embodiments, each R3 is independently hydrogen or C(R8)2C(O)N(R9)2, wherein each R8 and R9 are independently hydrogen, C1-C30 alkyl, or aryl. In certain embodiments, each R8 and R9 are independently hydrogen or C1-C6 alkyl.
In various embodiments, Z is OR4 or NHR4. In some embodiments, each R4 is independently hydrogen or substituted or unsubstituted C1-C30 alkyl. In further embodiments, each R4 is independently hydrogen or C1-C30 alkyl (e.g., C1-C20 alkyl, C1-C10 alkyl, or C1-C6 alkyl), SO2R10, or C(O)R10, wherein R10 is a C1-C10 alkyl or haloalkyl. In some embodiments, Z is OH, NHSO2CF3, NHC(O)CF3, or NHC(O)CH3. In certain embodiments, Z is NHC(O)CH3.
In various embodiments, n is independently an integer from 0 to 3. In some embodiments, n is 2. In certain embodiments, p is independently an integer from 0 to 3 and/or q is independently an integer from 1 to 3. In some embodiments, p is 0.
In various embodiments, each A is CR5 or N and each R5 is independently H, O, CN, OR7, NO2, C(R7)2OR7, C(O)(R7)2, CO2R7, N(R7)2, N(SO2R7)2, SO2R7, NR7C(O)R7, NR7C(R7)2OR7, NR7CO2R7, NR7C(O)N(R7)2, NR7SO2R7, CON(R7)2, SO2N(R7)2, OSO2R7,N(R7)SO2N(R7)2, halo, substituted or unsubstituted haloalkyl, or substituted or unsubstituted aryl. In some embodiments, each R5 is independently H, O, CN, NO2, OR7, CH2OR7, CO(R7)2, CO2R7, N(R7)2, SO2R7, NR7C(O)R7, NR7CO2R7, NR7SO2R7, CON(R7)2, OSO2R7, N(R7)SO2N(R7)2, N(SO2R7)2, halo, haloalkyl, or substituted or unsubstituted aryl. In further embodiments, each R5 is independently H, F, Cl, O, NO2, CONHCH3, OH, OCH3, CO(CH3)2, CONH2, CN, CF3, NHSO2CH3, NHSO2CF3, NHSO2N(R7)2CO2H, CO2CH3, CO2CH2CH3, SO2CH3, NHCOCH3, OSO2CH3, NHSO2R7, N(SO2R7)2, benzyl, phenyl, a hydroxyl-substituted phenyl (e.g., a radical of phenol, pyrocatechol or benzene-1,2, 3-triol) or a alkoxyl-substituted phenyl (e.g., a radical of 2-methyoxyphenol).
In various embodiments, each B is independently 0, S, C(O), or NR6. In some embodiments, R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted aryl, CN, OR7, C(R7)2OR7, C(O)R7, CO2R7, CON(R7)2, SO2R7, C(O)R7, SO2N(R7)2, or OSO2R7. In certain embodiments, each R6 is independently hydrogen, substituted or unsubstituted aryl, CN, OR7, CH2OR7, C(O)(R7)2, CO2R7, CF3, or CON(R7)2.
In various embodiments, each R7 is independently hydrogen, substituted or unsubstituted C1-C30 alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted haloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted aralkyl. In some embodiments, each R7 is independently hydrogen, phenyl, benzyl, halo-substituted phenyl, CF3, C1-C30 alkyl, or a C3-C12 cycloalkyl (e.g., cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl).
In various embodiments, when the compound of Formula (I) has the following structure:
then R1 is a substituent of Formula (III), (IV), (V), (VI), (VIII), (IXa), or (XIII):
In other embodiments, when the compound of Formula (I) has the following structure:
then R1 is a substituent of Formula (III), (IV), (V), (VI), (VII), (VIII), (IX), (IXa), (X), (XI), (XII), or (XIII):
In various embodiments, R1 is selected from the group consisting of:
In various embodiments, R1 is selected from the group consisting of:
In various embodiments, the compound of Formula (I) is selected from the group consisting of:
In various embodiments, the compound is selected from the group consisting of:
In various embodiments, the compound is selected from the group consisting of:
In various embodiments, the compound is capable of inhibiting FmlH. In some embodiments the compound can achieve at least 10% inhibition of FmlH. In some embodiments, the compound can achieve at least 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% inhibition of FmlH at a concentration of 100 nM.
As used herein, the term “hydrocarbyl” refers to hydrocarbyl moieties containing, for example, 1 to about 50 carbon atoms, 1 to about 30 carbon atoms, or 1 to about 20 carbon atoms, including branched or unbranched species, saturated or unsaturated species, and cyclic or acyclic species. Preferred hydrocarbyl can be selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylamino, thioalkyl, haloalkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, N-heterocyclyl, heterocyclylalkyl, aryl, aralkyl heteroaryl, N-heteroaryl, heteroarylalkyl, and the like. A hydrocarbyl may be optionally substituted (i.e., “substituted hydrocarbyl). Hence, various hydrocarbyls can be further selected from substituted alkyl, substituted cycloalkyl, substituted aryl, and so on.
The term “hydrocarbylene” as used herein describes radicals joined at two ends thereof to other radicals in an organic compound, and which consists of the elements carbon and hydrogen. These moieties include alkylene, alkenylene, alkynylene, and arylene moieties. These moieties also include alkyl, alkenyl, alkynyl, and aryl moieties substituted with other aliphatic or cyclic hydrocarbon groups, such as alkaryl, alkenaryl and alkynaryl. These moieties can contain, for example, 1 to about 50 carbon atoms, 1 to about 30 carbon atoms, or 1 to about 20 carbon atoms.
The substituted hydrocarbyl and hydrocarbylene moieties described herein are substituted with at least one atom other than carbon, including moieties in which a carbon chain atom is substituted with a hetero atom such as nitrogen, oxygen, silicon, phosphorous, boron, sulfur, or a halogen atom.
The term “aryl” as used herein alone or as part of another group denote optionally substituted homocyclic aromatic groups, preferably monocyclic or bicyclic groups containing from 6 to 12 carbons in the ring portion, such as phenyl, biphenyl, naphthyl, substituted phenyl, substituted biphenyl or substituted naphthyl. The term “aralkyl” as used herein denotes a group containing both alkyl and aryl structures such as benzyl.
In various aspects, the present invention generally relates to pharmaceutical compositions comprising a therapeutically effective amount of at least one compound of Formula (I) or salt or prodrug thereof:
wherein:
each X is independently hydrogen, fluoro, or OR2;
each R2 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Y is O, S, substituted or unsubstituted hydrocarbylene, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted thio, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2,
each R3 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
Z is hydrogen, OR4, SR4, or N(R4)2 (e.g., preferably NHR4);
each R4 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each m is independently an integer from 0 to 10; and
R1 is a substituent of Formula (II), (III), (IV), (V), (VI), (VII), (VIII), (IX), (IXa), (X), (XI), (XII) or (XIII):
wherein:
each A is independently CR5 or N;
each B is independently 0, S, C(O), C(R5)2, or NR6;
each R5 is independently hydrogen, oxygen, halo, substituted or unsubstituted amino, substituted or unsubstituted amido, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)pNO2, (CH2)pCN, (CH2)pOR7, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)pN(R7)2, (CH2)pN(SO2R7)2, (CH2)pSO2R7, (CH2)pNR7C(O)R7, (CH2)pNR7C(R7)2OR7, (CH2)pNR7CO2R7, (CH2)pNR7C(O)N(R7)2, (CH2)pNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)pN(R7)SO2N(R7)2 or (CH2)pOSO2R7;
each R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted hydrocarbyl, substituted or unsubstituted aryl, (CH2)qNO2, (CH2)pCN, (CH2)pOR7, (CH2)qN(SO2R7)2, (CH2)pC(R7)2OR7, (CH2)pC(O)(R7)2, (CH2)pCO2R7, (CH2)qN(R7)2, (CH2)pSO2R7, (CH2)qNR7C(O)R7, (CH2)pNR7C(R7)2OR7, (CH2)qNR7CO2R7, (CH2)qNR7C(O)N(R7)2, (CH2)qNR7SO2R7, (CH2)pCON(R7)2, (CH2)pSO2N(R7)2, (CH2)qN(R7)SO2N(R7)2, or (CH2)pOSO2R7;
each R7 is independently hydrogen or substituted or unsubstituted hydrocarbyl;
each n is independently an integer from 0 to 10;
each p is independently an integer from 0 to 10; and
each q is independently an integer from 1 to 10.
In various embodiments, the pharmaceutical composition comprises at least one compound of Formula (I) wherein each X is independently OH or CR2. In some embodiments, each R2 is independently hydrogen or substituted or unsubstituted C1-C30 alkyl. In some embodiments each R2 is independently hydrogen or a C1-C30 alkyl.
In various embodiments, the pharmaceutical composition comprises at least one compound of Formula (I) wherein Y is O, S, C(R3)2, (CH2)m, N(R3), N(H)R3, CO2, COOR3, SO2, SO2R3, (CH2)mO, O(CH2)m, (CH2)mS, S(CH2)m, C(O), C(O)N(R3), N(R3)C(O), R3N(R3)C(O), C(O)N(R3)R3, SO2N(R3), or N(R3)SO2. In some embodiments, m is independently an integer between 0 and 5. In various embodiments, each R3 is independently hydrogen or substituted or unsubstituted C1-C30 alkyl. In some embodiments each R3 is independently hydrogen or C(R8)2C(O)N(R9)2, wherein each R8 and R9 are independently hydrogen, C1-C30 alkyl, or aryl. In some embodiments, R8 and R9 are independently hydrogen or C1-C6 alkyl. In some embodiments, Y is N-(tert-butyl)-2-(N-methylacetamido) acetamide.
In various embodiments, the pharmaceutical composition comprises at least one compound of Formula (I) wherein Z is OR4 or NHR4. In some embodiments, each R4 is independently hydrogen or substituted or unsubstituted C1-C30 alkyl. In certain embodiments, each R4 is independently hydrogen, C1-C30 alkyl (e.g., C1-C20 alkyl, C1-C10 alkyl, or C1-C6 alkyl), SO2R10, or C(O)R10, wherein R10 is a C1-C10 alkyl or haloalkyl. In some embodiments, Z is OH, NHSO2CF3, NHC(O)CF3, or NHC(O)CH3. In some embodiments, Z is NHC(O)CH3.
In various embodiments, the pharmaceutical composition comprises at least one compound of Formula (I) wherein n is independently an integer from 0 to 3. In some embodiments, n is 2. In further embodiments, each p is independently an integer from 0 to 3 and/or each q is independently an integer from 1 to 3. In some embodiments, p is 0.
In various embodiments, the pharmaceutical composition comprises at least one compound of Formula (I) wherein each A is N or CR5 and each R5 is independently H, O, CN, OR7, NO2, C(R7)2OR7, C(O)(R7)2, CO2R7, N(R7)2, SO2R7, NR7C(O)R7, NR7C(R7)2OR7, NR7CO2R7, NR7C(O)N(R7)2, NR7SO2R7, CON(R7)2, SO2N(R7)2, OSO2R7, N(R7)SO2N(R7)2, N(SO2R7)2, halo, substituted or unsubstituted haloalkyl, or substituted or unsubstituted aryl. In some embodiments, each R5 is independently H, O, CN, NO2, OR7, CH2OR7, CO(R7)2, CO2R7, N(R7)2, SO2R7, NR7C(O)R7, NR7CO2R7, NR7SO2R7, CON(R7)2, OSO2R7, N(R′)SO2N(R7)2, N(SO2R7)2, halo, haloalkyl, or substituted or unsubstituted aryl. In further embodiments, each R5 is independently H, F, Cl, O, NO2, CONHCH3, OH, OCH3, CO(CH3)2, CONH2, CN, CF3, NHSO2CH3, NHSO2CF3, CO2H, CO2CH3, CO2CH2CH3, SO2CH3, NHCOCH3, OSO2CH3, NHSO2R7, N(SO2R7)2, benzyl, phenyl, a hydroxyl-substituted phenyl (e.g., a radical of phenol, pyrocatechol or benzene-1,2, 3-triol) or a alkoxyl-substituted phenyl (e.g., a radical of 2-methyoxyphenol).
In various embodiments, each B is independently 0, S, C(O), or NR6. In some embodiments, each R6 is independently hydrogen, substituted or unsubstituted haloalkyl, substituted or unsubstituted aryl, CN, OR7, C(R7)2OR7, C(O)R7, CO2R7, CON(R7)2, SO2R7, C(O)R7, SO2N(R7)2, or OSO2R7. In certain embodiments each R6 is independently hydrogen, substituted or unsubstituted aryl, CN, OR7, CH2OR7, C(O)(R7)2, CO2R7, CF3, or CON(R7)2.
In further embodiments, R7 is independently hydrogen, substituted or unsubstituted C1-C30 alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted haloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted aralkyl. In some embodiments, each R7 is independently hydrogen, C1-C30 alkyl, CF3, phenyl, benzyl, halo-substituted phenyl, or C3-C12 cycloalkyl (e.g., cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl).
In various embodiments, the pharmaceutical composition comprises a compound of Formula (I) wherein R1 of Formula (I) is selected from the group consisting of:
In various embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a compound selected from the group consisting of:
In various embodiments, the pharmaceutical composition comprises a compound selected from the group consisting of:
In various embodiments, the pharmaceutical composition comprises at least one compound selected from the group consisting of:
In various embodiments, the at least one compound is capable of inhibiting FmlH. In some embodiments the compound can achieve at least 10% inhibition of FmlH. In some embodiments, the compound can achieve at least 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% inhibition of FmlH at a concentration of 100 nM.
In various embodiments, the pharmaceutical compositions can further comprise at least one additional active ingredient. In some embodiments, the active ingredient comprises an antibacterial. Antibacterials include, for example, trimethoprim, sulfamethoxazole, fosfomycin, nitrofurantoin, cephalexin, ceftriaxone, amoxicillin, ceftazidime, clavulanate, avibactam, ceftolozane, tazobactam or any combination thereof. In some embodiments, the antibacterial comprises at least one cephalosporin. In some embodiments, the cephalosporin comprises cefpodoxime, cefdinir, cefaclor or any combination thereof. In some embodiments, the antibacterial comprises at least one fluoroquinolone. In some embodiments, the fluoroquinolone comprises ciprofloxacin and/or levofloxacin.
In additional embodiments, the antibacterial comprises an inhibitor of FimH. Effective inhibitors of FimH include mannoside based compounds. Such mannoside inhibitors are described in U.S. Pat. Nos. 8,937,167; 9,567,362; and US 2017/0247401, which are hereby incorporated by reference herein.
Pharmaceutical compositions containing one or more of the compounds described herein can be formulated in any conventional manner. Proper formulation is dependent in part upon the route of administration selected. Routes of administration include, but are not limited to, oral, parenteral (e.g., intravenous, intra-arterial, subcutaneous, rectal, subcutaneous, intramuscular, intraorbital, intracapsular, intraspinal, intraperitoneal, or intrasternal), topical (nasal, transdermal, intraocular), intravesical, intrathecal, enteral, pulmonary, intralymphatic, intracavital, vaginal, transurethral, intradermal, aural, intramammary, buccal, orthotopic, intratracheal, intralesional, percutaneous, endoscopical, transmucosal, sublingual and intestinal administration.
As used herein, “a therapeutically effective amount” is an amount capable of causing a pharmaceutical effect in a subject thereof with minimal side effects. In various cases the pharmaceutical effect of “an effective amount” is a reduction or depletion in the colonization of any type of pathological bacteria in human tissue.
Typically, the normal dosage amount of the inhibitor can vary from about 0.05 to about 500 mg per kg body weight depending upon the route of administration. The active ingredient can be administered in a single dose per day, or alternatively, in divided doses (e.g., twice per day, three times a day, four times a day, etc). In general, lower doses can be administered when a parenteral route is employed. Thus, for example, for intravenous administration, a dose in the range, for example, from about 0.05 mg to about 30 mg, from about 0.1 mg to about 25 mg, or from about 0.1 mg to about 20 mg per kg body weight can be used.
In various embodiments, the pharmaceutical compositions can comprise at least one excipient. Pharmaceutically acceptable excipients for use in the compositions of the present invention are selected based upon a number of factors including the particular compound used, and its concentration, stability and intended bioavailability; the subject, its age, size and general condition; and the route of administration.
The pharmaceutical compositions can be formulated, for example, for oral administration. The pharmaceutical compositions can be formulated as tablets, dispersible powders, pills, capsules, gel-caps, granules, solutions, suspensions, emulsions, syrups, elixirs, troches, lozenges, or any other dosage form that can be administered orally. Pharmaceutical compositions can include one or more pharmaceutically acceptable excipients. Suitable excipients for solid dosage forms include sugars, starches, and other conventional substances including lactose, talc, sucrose, gelatin, carboxymethylcellulose, agar, mannitol, sorbitol, calcium phosphate, calcium carbonate, sodium carbonate, kaolin, alginic acid, acacia, corn starch, potato starch, sodium saccharin, magnesium carbonate, microcrystalline cellulose, colloidal silicon dioxide, croscarmellose sodium, talc, magnesium stearate, and stearic acid. Further, such solid dosage forms can be uncoated or can be coated to delay disintegration and absorption.
The pharmaceutical compositions can also be formulated for parenteral administration, e.g., formulated for injection via intravenous, intra-arterial, subcutaneous, rectal, subcutaneous, intramuscular, intraorbital, intracapsular, intraspinal, intraperitoneal, or intrasternal routes. Dosage forms suitable for parenteral administration include solutions, suspensions, dispersions, emulsions or any other dosage form that can be administered parenterally.
Pharmaceutically acceptable excipients are identified, for example, in The Handbook of Pharmaceutical Excipients, (American Pharmaceutical Association, Washington, D.C., and The Pharmaceutical Society of Great Britain, London, England, 1968). Additional excipients can be included in the pharmaceutical compositions of the invention for a variety of purposes. These excipients can impart properties which enhance retention of the compound at the site of administration, protect the stability of the composition, control the pH, facilitate processing of the compound into pharmaceutical compositions, and so on. Other excipients include, for example, fillers or diluents, surface active, wetting or emulsifying agents, preservatives, agents for adjusting pH or buffering agents, thickeners, colorants, dyes, flow aids, non-volatile silicones, adhesives, bulking agents, flavorings, sweeteners, adsorbents, binders, disintegrating agents, lubricants, coating agents, and antioxidants.
As used herein, the term “prodrug” refers to a derivative of a compound that can hydrolyze, oxidize, or otherwise react under biological conditions (in vitro or in vivo) to provide a compound as described herein. Prodrugs may only become active upon some reaction under biological conditions, but they may have activity in their unreacted forms. Examples of prodrug moieties include substituted and unsubstituted, branch or unbranched lower alkyl ester moieties, (e.g., propionoic acid esters), lower alkenyl esters, di-lower alkyl-amino lower-alkyl esters (e.g., dimethylaminoethyl ester), acylamino lower alkyl esters (e.g., acetyloxymethyl ester), acyloxy lower alkyl esters (e.g., pivaloyloxymethyl ester), aryl esters (phenyl ester), aryl-lower alkyl esters (e.g., benzyl ester), substituted (e.g., with methyl, halo, or methoxy substituents) aryl and aryl-lower alkyl esters, amides, lower-alkyl amides, di-lower alkyl amides, and hydroxy amides. Prodrugs and their uses are well known in the art (see, e.g., Berge, et al. 1977 J. Pharm. Sci. 66:1-19). Prodrugs can typically be prepared using well-known methods, such as those described in Burger's Medicinal Chemistry and Drug Discovery (1995, Manfred E. Wolff ed., 5th ed. 172-178, 931-932).
“Salt” as used herein refers to pharmaceutically acceptable salts of the compounds described herein which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). Examples of pharmaceutically acceptable salts include, but are not limited to, nontoxic acid addition salts which are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate and aryl sulfonate.
The compounds of Formula (I) are useful for inhibiting FmlH. FmlH is a component of F9 pilus, an important urovirulance factor employed by uropathogenic E. coli (UPEC) for the maintenance of urinary tract infections (UTI). Deletion of FmlH in the urosepsis isolate, CFT073, results in a competitive defect for UPEC to maintain UTI in mouse models of the disease.
Accordingly, in various embodiments, the invention is directed to methods of treating bacterial infections in a subject in need thereof comprising administering to the subject a therapeutically effective amount of at least one compound of Formula (I). In some embodiments, the method of treating bacterial infections in the subject comprises administering to the subject any pharmaceutical composition described herein.
In various embodiments, the method of treating bacterial infections further comprises administering one or more additional active ingredients. In various embodiments the method further comprises administering an antibacterial. The antibacterial can comprise any antibacterial understood by practitioners in the art to be suitable for the specific infection. In some embodiments, the antibacterial comprises trimethoprim, sulfamethoxazole, fosfomycin, nitrofurantoin, cephalexin, ceftriaxone, amoxicillin, ceftazidime, clavulanate, avibactam, ceftolozane, tazobactam or any combination thereof. In some embodiments, the antibacterial comprises at least one cephalosporin. In some embodiments, the cephalosporin comprises cefpodoxime, cefdinir, cefaclor or any combination thereof. In some embodiments, the antibacterial comprises at least one fluoroquinolone. In some embodiments, the fluoroquinolone comprises ciprofloxacin or levofloxacin.
In various embodiments, the antibacterial comprises an inhibitor of FimH. Effective inhibitors of FimH include mannoside based compounds as described herein.
In various embodiments, the bacterial infections to be treated by the methods described herein can be urinary tract infections (e.g., cystitis), kidney infections (e.g. pyelonephritis).
The following non-limiting examples are provided to further illustrate the present invention.
The following examples describe the steps employed to design and test the novel galactoside inhibitors of FmlH of the current invention.
The compounds used in the following examples were synthesized and analyzed using the following protocols which will be referred to in later examples.
Starting materials, reagents, and solvents were purchased from commercial vendors unless otherwise noted. In general, anhydrous solvents were used for carrying out all reactions. 1H NMR spectra were measured on a Varian 400 MHz NMR instrument equipped with an auto sampler. The chemical shifts were reported as 8 ppm relative to TMS using residual solvent peak as the reference unless otherwise noted. The following abbreviations were used to express the peak multiplicities: s=singlet; d=doublet; t=triplet; q=quartet; m=multiplet; br=broad. High-performance liquid chromatography (HPLC) was carried out on GILSON GX-281 using Waters C18 5 μM, 4.6*50 mm and Waters Prep C18 5 μM, 19*150 mm reverse phase columns, eluted with a gradient system of 5:95 to 95:5 acetonitrile:water with a buffer consisting of 0.05-0.1% TFA. Mass spectroscopy (MS) was performed on HPLC/MSD using a gradient system of 5:95 to 95:5 acetonitrile:water with a buffer consisting of 0.05-0.1% TFA on a C18 or C8 reversed phased column and electrospray ionization (ESI) for detection. All reactions were monitored by thin layer chromatography (TLC) carried out on either Merck silica gel plates (0.25 mm thick, 60F254) or Millipore Silica gel aluminum sheets (60F254) and visualized by using UV (254 nm) or dyes such as KMnO4, p-Anisaldehyde and CAM (Hannesian's Stain). Molecular sieves (3 Å) were crushed and activated in vacuo at 390° C. overnight, then stored in a drying oven (300° C.) until just prior to use. Silica gel chromatography was carried out on a Teledyne ISCO CombiFlash purification system using pre-packed silica gel columns (12 g˜330 g sizes). All compounds used for biological assays are greater than 95% pure based on NMR and HPLC by absorbance at 220 nm and 254 nm wavelengths.
The two main reaction schemes to synthesize the galactosides and galactosamines used in Examples 18-28 are summarized herein:
Reaction scheme A describes boron trifluoride promoted glycosidation of protected galactose followed by deprotection to yield galactosides. This process was used to synthesize compounds 1-3, 5-9, 18-19 and 28-32β-Gal and 2-3, 18-19 and 28-32α-Gal. Reaction scheme B shows the Koenigs-Knorr type reaction followed by deprotection for the substitution of a galactosyl halide with an alcohol to yield galactosides and galactosaminides. 20β-GalNac, 21β-Gal, 30β-GalNAc, and 29β-GalNac were produced using this procedure. The aglycone “R” groups that identify each of the galactosides/galactosamines are described in
The common details of the glycosylation and deacetylation protocols used to synthesize these compounds are described below. Specific changes relevant to given compounds are described in later examples.
Glycosylation Protocol A: Starting from Galactose 1,2,3,4,6 Penta-O-Acetyl-β-D-Galactose.
Under nitrogen atmosphere, a solution of 1,2,3,4,6-penta-O-acetyl-β-D-galactose (0.25 mmol), phenol derivative (0.50 mmol), and 3 Å molecular sieves was stirred in either CH2Cl2 or 1,2-dichloroethane (5 mL) for 1 h. Boron trifluoride diethyl etherate (0.75 mmol) was then added dropwise, and the solution was stirred for the specified time and temperature, monitoring by TLC and LCMS. Upon completion, the reaction was cooled to room temperature (rt) and neutralized with Et3N. The sieves were filtered off, and the remaining filtrate was washed with sat. aq. NaHCO3 (2×1 mL), and brine (1×1 mL), dried over Na2SO4, and concentrated in vacuo. The resulting residue was then purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, to give the glycosylation product.
Glycosylation Protocol B: Starting from 2,3,4,6-Tetra-O-Acetyl-α-D-Galactopyranosyl Bromide
Under nitrogen atmosphere, 1,2-dichloroethane (2 mL) was added to a flask containing 2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl bromide (103 mg, 0.25 mmol) and phenol derivative (0.75 mmol). Silver carbonate (0.50 mmol) was then added, and the solution was stirred for specified time and temperature, monitoring by TLC and LCMS. Upon completion, the reaction was cooled, filtered and concentrated in vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, to give the glycosylation product.
Glycosylation Protocol C: Starting from 2-Acetamido-3,4,6-Tri-O-Acetyl-2-Deoxy-α-D-Galactopyranosyl chloride (biphasic conditions)
1N aqueous NaOH solution (1 mL) was added into a solution of 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-α-D-galactopyranosyl chloride (100 mg, 0.273 mmol), tetrabutylammonium bromide (88 mg, 0.273 mmol) and phenol derivative (0.546 mmol) in dichloromethane (2 mL) at room temperature. The reaction was stirred until the TLC indicated complete disappearance of chloride. The reaction was then diluted with dichloromethane (10 mL) and washed with water, followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated in vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, to give the glycosylation product.
Glycosylation Protocol D: Starting from 2,3,4,6-Tetra-O-Acetyl-α-D-Galactopyranosyl Bromide (Biphasic Conditions)
1N aqueous NaOH solution (1 mL) was added into a solution of 2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl bromide (200 mg, 0.487 mmol), benzyltriethylammonium chloride (111 mg, 0.0.487 mmol) and phenol derivative (0.975 mmol) in chloroform (2 mL) at room temperature. The reaction was stirred at 60° C. until the TLC indicated complete disappearance of starting material. The reaction was cooled and diluted with dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, to give the glycosylation product.
Acetyl esters were removed by dissolving the galactoside intermediate (0.5 mmol) in 20 mL of methanol, with a catalytic amount of sodium methoxide (0.02 M), and stirred overnight at room temperature. Upon completion, H+ exchange resin (DOWEX 50WX4-100) was added to neutralize the mixture. The resin was filtered off and the filtrate was concentrated and then dried in vacuo, and the residue was purified by HPLC (C18, 15*150 mm column; eluent: acetonitrile/water (0.05% TFA).
33% Wt. Methylamine in absolute ethanol solution (5 mL) was added to the galactoside intermediate (0.105 mmol), and the reaction was stirred at room temperature (0.5-1 h) until TLC indicated complete disappearance of the staring material. Complete evaporation of the solvent provides the pure compound, which was further purified by HPLC (C18, 15*150 mm column; eluent: acetonitrile/water (0.05% TFA).
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 2-methylphenol (0.052 mL, 0.51 mmol) in 1,2-dichloroethane (rt for 10 h), to give glycosylation product, 2-methylphenyl 2,3,4,6-tetra-O-acetyl-α/13-D-galactopyranoside, in 14% yield (alpha); and 6% yield (beta); ESI-MS [M+Na]+ calcd for C21H26O10Na+461.14, found 461.3 (alpha) and 461.3 (beta). Subsequently, the acetates were removed using the deprotection protocol “A” described in Example 1 to give the title compounds (2α), and separately (2β), each in quantitative yield. Analytical data for (2α): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.15-7.19 (m, 1H), 7.07-7.14 (m, 2H), 6.82-6.92 (m, 1H), 5.52 (d, J=2.7 Hz, 1H), 3.97-4.01 (m, 3H), 3.93 (t, J=6.1 Hz, 1H), 3.62-3.75 (m, 2H), 2.28 (s, 3H); ESI-MS [M+Na]+ calcd for C13H18O6Na+293.10, found 293.3. Analytical data for (213): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.05-7.20 (m, 3H), 6.86-6.94 (m, 1H), 4.85 (d, Hz, 1H), 3.89-3.93 (m, 1H), 3.83 (dd, J=9.8, 7.8 Hz, 1H), 3.76 (d, J=2.0 Hz, 1H), 3.75 (s, 1H), 3.63-3.67 (m, 1H), 3.57 (dd, J=9.8, 3.5 Hz, 1H), 2.27 (s, 3H); ESI-MS [M+Na]+ calcd for C13H18O6Na+293.10, found 293.3.
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 2-cyanophenol (0.061 g, 0.51 mmol) in 1,2-dichloroethane (rt for 10 h), to give glycosylation product, 2-cyanophenyl 2,3,4,6-tetra-O-acetyl-α/13-D-galactopyranoside, in 10% yield (alpha) and 10% yield (beta); ESI-MS [M+Na]+ calcd for C21H23NO10Na+472.12, found 472.3 (alpha) and 472.3 (beta). The acetates were subsequently removed using the deprotection protocol “A” described in Example 1 to give the title compounds (3a), and separately (3β), each in quantitative yield. Analytical data for (3a): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.56-7.66 (m, 2H), 7.42 (d, J=8.2 Hz, 1H), 7.10-7.17 (m, 1H), 5.78 (d, J=3.1 Hz, 1H), 4.03-4.10 (m, 2H), 4.00-4.03 (m, 1H), 3.93 (t, J=6.1 Hz, 1H), 3.61-3.72 (m, 2H); ESI-MS [M+H]+ calcd for C13H15NO6H+282.10, found 282.3. Analytical data for (3β): 1H NMR (400 MHz, dimethylsulfoxide-d6) δ ppm 7.67 (dd, J=7.8, 1.6 Hz, 1H), 7.58 (ddd, J=8.9, 7.3, 1.8 Hz, 1H), 7.27 (d, J=8.6 Hz, 1H), 7.01-7.12 (m, 1H), 5.15 (d, J=5.5 Hz, 1H), 5.04 (d, J=7.8 Hz, 1H), 4.83 (d, J=5.9 Hz, 1H), 4.58 (t, J=5.5 Hz, 1H), 4.53 (d, J=4.3 Hz, 1H), 3.63-3.67 (m, 1H), 3.52-3.61 (m, 1H), 3.32-3.51 (m, 4H); ESI-MS [M+H]+ calcd for C13H15NO6H+282.10, found 282.3
Following glycosylation protocol “B”, described in Example 1, acetobromogalactose tetraacetate (0.100 g, 0.24 mmol) was coupled with guaiacol (0.090 g, 0.73 mmol) in 1,2-dichloroethane (40° C. for 1 h), to give glycosylation product, 2-cyanophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 16% yield; ESI-MS [M+Na]+ calcd for C21H26O11Na+ 477.14, found 477.3. The acetates were subsequently removed via the deprotection protocol “A” described in Example 1 to give the title compound (5β) in quantitative yield. Analytical data for (513): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.18 (d, J=7.4 Hz, 1H), 6.97-7.01 (m, 2H), 6.86-6.91 (m, 1H), 4.85 (d, 1H), 3.89 (d, J=3.5 Hz, 1H), 3.85 (s, 3H), 3.81-3.84 (m, 1H), 3.76 (d, J=2.0 Hz, 1H), 3.74 (s, 1H), 3.62-3.66 (m, 1H), 3.58 (dd, J=9.8, 3.5 Hz, 1H); ESI-MS [M+Na]+ calcd for C13H18O7Na+309.10, found 309.3.
Following glycosylation protocol “B”, described in Example 1, acetobromogalactose tetraacetate (0.100 g, 0.24 mmol) was coupled with 2-chlorophenol (0.074 mL, 0.73 mmol) in 1,2-dichloroethane (40° C. for 1 h), to give the glycosylation product, 2-chlorophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 23% yield; ESI-MS [M+Na]+ calcd for C20H23ClO10Na+481.09, found 481.3. The acetates were subsequently removed via the deprotection protocol “A” described in Example 1 to give the title compound (6β) in quantitative yield. Analytical data for (6β): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.32-7.38 (m, 1H), 7.19-7.29 (m, 2H), 6.93-7.02 (m, 1H), 4.95 (d, J=7.4 Hz, 1H), 3.92 (d, J=3.5 Hz, 1H), 3.88 (dd, J=9.4, 7.8 Hz, 1H), 3.72-3.82 (m, 2H), 3.66-3.71 (m, 1H), 3.59 (dd, J=9.4, 3.1 Hz, 1H); ESI-MS [M+Na]+ calcd for C42H43N3O6Na+ 313.05, found 313.2.
Following glycosylation protocol “B”, described in Example 1, acetobromogalactose tetraacetate (0.100 g, 0.24 mmol) was coupled with 3,5-dimethoxyphenol (0.113 g, 0.73 mmol) in 1,2-dichloroethane (40° C. for 1 h), to give the glycosylation product, 3,5-dimethoxyphenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 8% yield; ESI-MS [M+Na]+ calcd for C22H28O12Na+507.15, found 507.3. The acetates were then removed using the deprotection protocol “A”, described in Example 1, to give the title compound (7β) in quantitative yield. Analytical data for (7β): 1H NMR (400 MHz, Methanol-d4) δ ppm 6.32 (d, J=2.0 Hz, 2H), 6.15 (t, J=2.2 Hz, 1H), 4.81 (d, J=7.8 Hz, 1H), 3.88 (d, J=3.1 Hz, 1H), 3.71-3.82 (m, 9H), 3.65-3.70 (m, 1H), 3.57 (dd, J=9.6, 3.3 Hz, 1H); ESI-MS [M+Na]+ calcd for C14H20O8Na+ 339.11, found 339.3.
Following glycosylation protocol “B”, described in Example 1, acetobromogalactose tetraacetate (0.100 g, 0.24 mmol), was coupled with p-cresol (0.077 mL, 0.73 mmol) in 1,2-dichloroethane (40° C. for 1 h), to give the glycosylation product, 4-methylphenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 3% yield; ESI-MS [M+Na]+ calcd for C21H26O10Na+461.14, found 461.3. The acetates were then removed via the deprotection protocol “A” described in Example 1 to give the title compound (8β) in quantitative yield. Analytical data for (8β): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.04-7.09 (m, 2H), 6.96-7.01 (m, 2H), 4.79 (d, J=7.8 Hz, 1H), 3.88-3.91 (m, 1H), 3.71-3.80 (m, 3H), 3.62-3.67 (m, 1H), 3.54-3.59 (m, 1H), 2.26 (s, 3H); ESI-MS [M+Na]+ calcd for C13H18O6Na+293.10, found 293.2.
Following glycosylation protocol “B”, described in Example 1, acetobromogalactose tetraacetate (0.100 g, 0.24 mmol) was coupled with 4-cyanophenol (0.087 g, 0.73 mmol) in 1,2-dichloroethane (40° C. for 1 h), to give the glycosylation product, 4-cyanophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 16% yield; ESI-MS [M+Na]+ calcd for C21H23NO10Na+472.12, found 472.3. The acetates were subsequently removed via the deprotection protocol “A” described in Example 1 to give the title compound (9β) in quantitative yield. Analytical data for (9β): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.66 (d, J=9.0 Hz, 2H), 7.23 (d, J=8.6 Hz, 2H), 4.97 (d, J=7.8 Hz, 1H), 3.91 (d, J=3.5 Hz, 1H), 3.70-3.86 (m, 4H), 3.60 (dd, J=9.8, 3.5 Hz, 1H); ESI-MS [M+H]+ calcd for C13H15NO6H+282.10, found 282.2
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 1-naphthol (0.074 g, 0.51 mmol) in CH2Cl2 (rt for 48 h), to give glycosylation product, 1-naphthalenyl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside, in 14% yield (alpha), and 40% yield (beta); ESI-MS [M+Na]+ calcd for C24H26O10Na+497.14, found 497.3 (alpha) and 497.3 (beta). The acetates were then removed via the deprotection protocol “A” described in Example 1 to give the title compounds (18a), and separately (1813), each in quantitative yield. Analytical data for (18α): 1H NMR (400 MHz, Methanol-d4) δ ppm 8.38 (ddt, J=6.3, 3.6, 0.8 Hz, 1H), 7.83-7.77 (m, 1H), 7.53-7.27 (m, 5H), 5.72 (d, J=3.7 Hz, 1H), 4.20-4.05 (m, 2H), 4.04 (dd, J=3.4, 1.3 Hz, 1H), 3.98 (td, J=6.1, 5.6, 1.3 Hz, 1H), 3.75-3.64 (m, 2H); ESI-MS [M+Na]+ calcd for C16H18O6Na+329.10, found 329.3. Analytical data for (18β): 1H NMR (400 MHz, Methanol-d4) δ ppm 8.43-8.36 (m, 1H), 7.83-7.76 (m, 1H), 7.53-7.41 (m, 3H), 7.37 (t, J=8.0 Hz, 1H), 7.22 (dd, J=7.7, 0.9 Hz, 1H), 5.07 (d, J=7.8 Hz, 1H), 4.02-3.92 (m, 2H), 3.84-3.70 (m, 3H), 3.63 (dd, J=9.7, 3.4 Hz, 1H); ESI-MS [M+Na]+ calcd for C16H18O6Na+329.10, found 329.3.
Following glycosylation protocol “D”, described in Example 1, 2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl bromide (200 mg, 0.487 mmol) was coupled with 8-hydroxy isoquinoline (141.53 mg, 0.975 mmol), to give glycosylation product, 8-isoquinolinyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside, in 86% yield; ESI-MS [M+H]+ calcd for C23H25NO10H+476.16, found 476.3 The acetates were then removed via deprotection protocol “B” to give the title compound (2113) in 87% yield. Analytical data for (21β): 1H NMR (400 MHz, Methanol-d4) δ ppm 9.69 (s, 1H), 8.44 (d, J=5.5 Hz, 2H), 7.89-7.90 (m, 1H), 7.68-7.81 (m, 2H), 7.57 (d, J=8.2 Hz, 1H), 7.40 (d, J=7.8 Hz, 1H), 5.16 (d, J=7.8 Hz, 1H), 3.99-4.07 (m, 1H), 3.97 (d, J=3.5 Hz, 1H), 3.76-3.85 (m, 3H), 3.67 (dd, J=9.8, 3.1 Hz, 1H); ESI-MS [M+H]+ calcd for C15H17NO6H+308.11, found 308.3.
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 2-phenylphenol (0.087 g, 0.51 mmol) in CH2Cl2 (rt for 48 h), to give glycosylation product, [1,1′-biphenyl]-2-yl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside, in 38% yield (alpha) and 15% yield (beta); ESI-MS [M+Na]+ calcd for C26H28O10Na+ 523.16, found 523.4 (alpha) and 523.4 (beta). The acetates were then removed via deprotection protocol “A”, to give the title compounds (28α), and separately (28β), each in quantitative yield. Analytical data for (28α): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.60-7.54 (m, 2H), 7.40 (t, J=7.6 Hz, 2H), 7.36-7.28 (m, 4H), 7.09 (m, 1H), 5.61 (d, J=3.7 Hz, 1H), 3.90 (m, 1H), 3.80 (d, J=3.4 Hz, 1H), 3.70 (m, 1H), 3.61-3.47 (m, 3H); ESI-MS [M+Na]+ calcd for C18H20O6Na+355.12, found 355.3. Analytical data for (2813): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.62-7.58 (m, 2H), 7.40-7.34 (m, 2H), 7.31-7.25 (m, 4H), 7.07 (m, 1H), 5.00 (d, J=7.7 Hz, 1H), 3.88 (dd, J=3.3, 1.0 Hz, 1H), 3.79-3.65 (m, 4H), 3.54 (dd, J=9.6, 3.4 Hz, 1H); ESI-MS [M+Na]+ calcd for C18H20O6Na+355.12, found 355.3.
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.200 g, 0.51 mmol) was coupled with 2′-hydroxy[1,1′biphenyl]-3-carboxylic acid methyl ester (0.234 g, 1.02 mmol) in CH2Cl2 (rt for 1.5 h), to give glycosylation product, 2′-(2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranosyloxy)-[1,1′-Biphenyl]-3-carboxylic acid methyl ester, in 5% yield (alpha) and 18% yield (beta); ESI-MS [M+Na]+ calcd for C28H30O12Na+581.16, found 581.4 (alpha) and 581.4 (beta). The acetates were then removed via deprotection protocol “A” to give the title compounds (30α) and separately (30β), each in quantitative yield. Analytical data for (30α): 1H NMR (400 MHz, Methanol-d4) δ 8.23 (m, 1H), 7.97 (m, 1H), 7.86 (m, 1H), 7.53 (tt, J=7.7, 0.7 Hz, 1H), 7.39-7.31 (m, 3H), 7.11 (m, 1H), 5.65 (d, J=3.7 Hz, 1H), 3.95-3.90 (m, 4H), 3.85 (d, J=3.4 Hz, 1H), 3.73 (dd, J=10.1, 3.4 Hz, 1H), 3.66-3.52 (m, 4H); ESI-MS [M+Na]+ calcd for C20H22O8Na+413.12, found 413.3. Analytical data for (30β): 1H NMR (400 MHz, Methanol-d4) δ 8.24 (m, 1H), 7.92 (m, 1.0 Hz, 2H), 7.50 (td, J=7.8, 0.6 Hz, 1H), 7.37-7.30 (m, 3H), 7.14-7.08 (m, 1H), 5.03 (d, J=7.7 Hz, 1H), 3.92 (d, J=0.8 Hz, 3H), 3.88 (dd, J=3.5, 0.9 Hz, 1H), 3.80-3.66 (m, 4H), 3.56 (ddd, J=9.6, 3.4, 0.7 Hz, 1H); ESI-MS [M+Na]+ calcd for C20H22O8Na+413.12, found 413.3.
Compound 30α (38 mg, 0.097 mmol) and separately 30β (38 mg, 0.097 mmol), produced as described in Example 12, were each dissolved in MeOH (2 mL) at rt. Then, [0.5 M] NaOH aq. (2 mL) was added dropwise, and the reactions were monitored by LCMS. After 24 h, the reactions were acidified with [0.2 N] HCl aq. to a pH of ˜3-4. The solvents were then evaporated under reduced pressure, and the compounds were purified by HPLC (C18, 15*150 mm column; eluent: acetonitrile/water (0.05% TFA) to give the title compounds (29α) in 5% yield, and separately (29β) in 88% yield. Analytical data for (29α): 1H NMR (300 MHz, methanol-d4) δ ppm 8.22 (d, J=1.6 Hz, 1H), 7.84-8.02 (m, 2H), 7.53 (td, J=7.7, 4.5 Hz, 1H), 7.29-7.40 (m, 3H), 7.06-7.16 (m, 1H), 5.63-5.69 (m, 1H), 3.89-3.96 (m, 1H), 3.85 (br. s., 1H), 3.72-3.78 (m, 1H), 3.54-3.67 (m, 3H); ESI-MS [M+Na]+ calcd for C19H20O8Na+399.11, found 399.6. Analytical data for (29β): 1H NMR (300 MHz, methanol-d4) δ ppm 8.16 (s, 1H), 7.87 (d, J=7.8 Hz, 1H), 7.81 (d, J=7.8 Hz, 1H), 7.41 (t, J=7.8 Hz, 1H), 7.19-7.30 (m, 3H), 6.98-7.06 (m, 1H), 4.94 (d, J=7.8 Hz, 1H), 3.80 (d, J=3.1 Hz, 1H), 3.55-3.72 (m, 4H), 3.48 (dd, J=9.6, 3.3 Hz, 1H); 13C NMR (100 MHz, methanol-d4) δ 170.1, 155.7, 140.1, 135.6, 132.0, 131.7, 131.6, 130.2, 129.1, 123.6, 116.6, 102.6, 77.0, 75.2, 72.3, 70.2, 62.4; ESI-MS [M+Na]+ calcd for C19H20O8Na+399.11, found 399.6.
Following glycosylation protocol “C”, described in Example 1, 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-α-D-galactopyranosyl chloride (100 mg, 0.273 mmol), was coupled with 2′-hydroxy[1,1′biphenyl]-3-carboxylic acid methyl ester (0.125 g, 0.546 mmol), to give glycosylation product, 2′-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-galactopyranosyloxy)-[1,1′-Biphenyl]-3-carboxylic acid methyl ester. The acetates were removed via deprotection protocol B, to give the title compound (30β-Nac) in 80% yield. Analytical data for (30β-Nac): 1H NMR (400 MHz, Methanol-d4) δ ppm 8.08 (s, 1H), 7.94 (d, J=7.4 Hz, 1H), 7.72 (d, J=7.4 Hz, 1H), 7.43-7.49 (m, 1H), 7.27-7.38 (m, 3H), 7.10 (t, J=7.2 Hz, 1H), 5.06 (d, J=8.6 Hz, 1H), 4.04 (t, J=9.6 Hz, 1H), 3.90 (s, 3H), 3.72-3.87 (m, 4H), 3.57-3.69 (m, 3H), 1.60 (s, 3H); ESI-MS [M+Na]+ calcd for C22H25NO8Na+ 454.15, found 454.3.
NaOH (79 mg, 1.97 mmol) was added into a solution of 30β-NAc (Example 13, 110 mg, 0.197 mmol) in 50% water in methanol (10 mL) at room temperature. The reaction was stirred at the same temperature (15 h) until the TLC indicated complete disappearance of the staring material. The reaction solution was neutralized with 6N aqueous HCl and the MeOH was evaporated in vacuo. The aqueous solution was adjusted to a pH˜2 with 6N aqueous HCl and the product was extracted with ethyl acetate (3×10 mL). The organic layers were combined and washed with brine, dried over Na2SO4, and concentrated in vacuo. The resulting residue was purified by silica gel chromatography with dichloromethane/methanol combinations as eluent provide the title compound (29β-NAc) in 67% yield. Analytical data for (29β-NAc): 1H NMR (300 MHz, methanol-d4) δ ppm 8.00 (s, 1H), 7.87 (d, J=7.8 Hz, 1H), 7.64 (d, J=7.4 Hz, 1H), 7.35-7.42 (m, 1H), 7.21-7.29 (m, 3H), 6.98-7.07 (m, 1H), 5.01 (d, J=8.6 Hz, 1H), 3.94 (dd, J=10.6, 8.6 Hz, 1H), 3.78 (d, J=3.1 Hz, 1H), 3.64-3.76 (m, 2H), 3.54-3.61 (m, 2H), 1.55 (s, 3H); ESI-MS [M+Na]+ calcd for C21H23NO8Na+440.13, found 440.3
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 3-phenylphenol (0.087 g, 0.51 mmol) in CH2Cl2 (rt for 72 h), to give glycosylation product, [1,1′-biphenyl]-3-yl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside, in 36% yield (alpha) and 23% yield (beta). Analytical data for [1,1′-biphenyl]-3-yl 2,3,4,6-tetra-O-acetyl-α-D-galactopyranoside: ESI-MS [M+Na]+ calcd for C26H28O10Na+523.16, found 523.4. Analytical data for [1,1′-biphenyl]-3-yl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside: ESI-MS [M+Na]+ calcd for C26H28O10Na+523.16, found 523.3. The acetates were removed via deprotection protocol A, to give the title compounds (31α), and separately (31β), each in quantitative yield. Analytical data for (31α): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.49 (m, 2H), 7.35-7.13 (m, 6H), 7.06 (m, 1H), 5.46 (d, J=2.7 Hz, 1H), 3.94-3.83 (m, 4H), 3.66-3.55 (m, 2H); ESI-MS [M+Na]+ calcd for C18H20O6Na+355.12, found 355.3. Analytical data for (31β): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.61-7.56 (m, 2H), 7.42-7.22 (m, 6H), 7.08 (m, 1H), 4.91 (d, J=7.7 Hz, 1H), 3.89 (dd, J=3.5, 0.9 Hz, 1H), 3.84-3.66 (m, 4H), 3.58 (dd, J=9.7, 3.4 Hz, 1H); ESI-MS [M+Na]+ calcd for C18H20O6Na+ 355.12, found 355.3.
Following glycosylation protocol “A”, described in Example 1, β-D-galactose pentaacetate (0.100 g, 0.26 mmol) was coupled with 4-phenylphenol (0.087 g, 0.51 mmol) in CH2Cl2 (rt for 72 h), to give glycosylation product, [1,1′-biphenyl]-4-yl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside, in 26% yield (alpha) and 14% yield (beta). Analytical data for [1,1′-biphenyl]-4-yl 2,3,4,6-tetra-O-acetyl-α-D-galactopyranoside: ESI-MS [M+Na]+ calcd for C26H28O10Na+523.16, found 523.3. Analytical data for [1,1′-biphenyl]-4-yl 2,3,4,6-tetra-O-acetyl-α/β-D-galactopyranoside: ESI-MS [M+Na]+ calcd for C26H28O10Na+523.16, found 523.3. Step 2: The acetates were removed via deprotection protocol A, to give the title compounds (32α), and separately (32β), each in quantitative yield. Analytical data for (32α): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.59-7.49 (m, 4H), 7.44-7.36 (m, 2H), 7.31-7.19 (m, 3H), 5.54 (d, J=2.7 Hz, 1H), 4.03-3.92 (m, 4H), 3.70 (dd, J=6.3 Hz, 2H); ESI-MS [M+Na]+ calcd for C18H20O6Na+355.12, found 355.3. Analytical data for (32β): 1H NMR (400 MHz, Methanol-d4) δ ppm 7.61-7.56 (m, 2H), 7.42-7.22 (m, 6H), 7.08 (m, 1H), 4.91 (d, J=7.7 Hz, 1H), 3.89 (dd, J=3.5, 0.9 Hz, 1H), 3.84-3.66 (m, 4H), 3.58 (dd, J=9.7, 3.4 Hz, 1H); ESI-MS [M+Na]+ calcd for C18H20O6Na+355.12, found 355.3.
An ELISA-based competition assay was performed to detect binding of FmlHLD to surface-immobilized desialylated bovine submaxillary mucin (ds-BSM) in the presence or absence of soluble compounds at 1 and 0.1 mM (unless otherwise noted): galactosidase (gal), N-acetyl galactosidase (GalNAc), TF, mannose (1 mM only), glucose (1 mM only), lactose (galactose-(β1-4-glucose, 1 mM only), o-nitrophenyl β-galactoside (ONPG, 0.1 mM only) and isopropyl β-thiogalactoside (IPTG, 0.1 mM only). Immulon 4HBX 96-well plates were coated overnight with 1 μg bovine submaxillary mucin (Sigma). Coated wells were then treated with 100 μl of Arthrobacter ureafaciens sialidase (AUS) (10 mU/ml) diluted in PBS for 1 hour at 37° C. Thereafter, wells were incubated with 200 μl blocking buffer (PBS+1% BSA) for 2 hours at 23° C., followed by incubation with 100 μl biotinylated FmlHLD diluted in blocking buffer to 20 μg/ml in the presence or absence of the tested compounds for 1 hr at 23° C. After washing 3× with PBS+0.05% TWEEN-20, 100 μl Streptavidin-HRP conjugate (BD Biosciences; 1:2000 dilution in blocking buffer) was added to each well for 1 hr at 23° C. After a final round of washing, plates were developed with 100 μl Tetramethylbenzidine (TMB, BD Biosciences) substrate, quenched within 1-2 min with 50 μl M H2504, and absorbance measured at 450 nm. The results are summarized in
To further understand the FmlHLD binding pocket to further the design of appropriate inhibitors, X-ray crystallography was implemented to elucidate the three-dimensional structures of apo and ligand-bound FmlHLD. The parameters used for data collection and analysis are summarized in Table 1, and described in detail below.
aRmerge = Σhkl Σi |Ii(hkl)-<I(hkl)>|/Σhkl ΣiIi(hkl), where the sum i is over all separate measurements of the unique reflection hkl.
bRpim = Σhkl [1/(n-1)]1/2 Σi|Ii(hkl)-<I(hkl) >|/Σhkl Σi Ii(hkl)
bRwork = Σhkl ||Fobs| - |Fcalc||/Σhkl |Fobs|
cRfree, calculated the same as for Rwork but on the 5% data randomly excluded from the refinement calculation.
First, a crystal structure of apo FmlHLD was solved at 1.6 Å resolution by molecular replacement (MR) using FimHLD (PDB ID 3MCY) as the search model. Briefly, crystals of apo FmlHLD in 10 mM Hepes pH 7.5, 50 mM NaCl were grown by mixing 2 μl protein (6 mg/ml) with 2 μl of mother liquor (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 28% PEG 3350) and equilibrated against 1 ml mother liquor in the reservoir. Additional co-crystals of FmlHLD bound to TF or ONPG were grown by mixing 2 μl protein (6 mg/ml) in the presence of 5 mM compound with 2 μl of mother liquor (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 32% PEG 3350). These crystals were transferred into cryoprotectant (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 35% PEG 4000, 10% glycerol) and then flash frozen in liquid nitrogen. Diffraction data for FmlHLD apo and TF were collected at 100 K at an in-house facility equipped with a rotating anode Rigaku MicroMax 007 generator, a Rayonix Marmux X-ray source (Evanston, Ill.), and a Mar345 image plate detector. Diffraction data for FmlHLD apo, ONPG were collected at 100 K at the ALS Beamline 4.2.2. Data were indexed and integrated in iMosflm [43], XDS [44], or HKL2000 and scaled by Scala [45]. The phase problem was solved by molecular replacement (MR) using Phaser-MR in PHENIX [46] with FimHLD from PDB ID 3MCY. Several rounds of refinements were performed in PHENIX to improve the final models. Within this structure, two copies of FmlHLD are found in the asymmetric unit, each of which adopts a canonical I3-sandwich fold, with three distinct binding loops (Loop 1: residues 10-15; Loop 2: residues 44-53; Loop 3: residues 132-142) that form a wide, shallow, solvent-exposed binding pocket (
The co-crystal structure of FmlHLD-TF reveals two copies of FmlHLD-TF in the unit cell, in which each TF adopts a distinct ligand conformation (
The FmlHLD-ONPG co-crystal structure also shows two copies of FmlHLD in the unit cell, in which a sulfate ion occupies the binding pocket of chain A while ONPG occupies the binding pocket of chain B (
A virtual library composed of approximately 1800 known galactosides was assembled by mining the ZINC12 database [29] for all known galactose sub-structures. Thereafter, an exhaustive virtual screen was performed using AutoDock Vina to computationally dock the galactoside library in the binding pocket of FmlHLD (from a FmlHLD-ONPG co-crystal structure; PDB ID 6AOY), generating a ranked list of top binding poses and associated docking scores for each galactoside. To identify top hits, the inventors abstained from directly comparing raw binding scores, as large, lipophilic molecules tend to have artificially high predicted binding interactions due to their contribution to hydrophobic interactions as calculated by the empirical scoring function of AutoDock Vina. Instead, the results of the virtual screen were evaluated per group efficiency (GE), which, in this context, measures the contribution of the aglycone group within each galactoside (X) to docking score (DS) with respect to the number of heavy atoms (HA) present in the aglycone group [GE=(DSX-DSGal)/(HAX-HAGal)]. Data are presented in
Thereafter, the binding poses of top hits were visually inspected to inform structure-guided drug design. In addition to the canonical binding observed for the Gal moiety of top-scoring galactosides in the cleft of the binding pocket, most top hits also interacted with specific hot spot residues near the galactose binding pocket, which were sought to leverage for lead optimization. These hot spot residues included (i) residue Y46, which caps the top of the binding pocket and can contribute hydrophobic interactions, (ii) residue K132, which lies at the bottom of the sugar binding pocket and can engage polar groups linked to the Gal sugar, and (iii) residue R142, which points towards an empty, solvent-exposed cleft near the binding pocket and can contribute electrostatic interactions (
To explore structure-activity relationships (SAR), a large library of galactoside analogs were acquired or synthesized (
Select top hit compounds and few low-scoring analogs from the virtual screening, as well as synthetic galactosides, were tested in the ELISA-based competition assay for their ability to inhibit binding of FmlHLD to ds-BSM. The ELISA assay was performed using the protocol described in Example 18. Direct comparison of inhibitory potency among galactosides led to delineation of basic SAR (Table 2, below). All compounds were tested at 100 μM; a subset was tested at 10 μM; and the strongest subset was additionally tested at 1 μM. The summary of the results are in Table 2. Percent inhibition (PI) values are reported as the mean with standard error of the mean for galactosides tested at 100 μM (PI100), 10 μM (PI10), and 1 μM (PI1) in the ELISA-based competition assay. Compounds indicated in the table are shown in
When tested at 100 μM, phenyl β-galactoside 1β (beta isomer of 1) exhibited significantly higher binding inhibition (77%) than Gal (8.1%), indicating that the phenyl group enhances binding to FmlHLD (Table 2). Various ortho-substituents on the phenyl ring additionally conferred substantial improvements in inhibitory potency, as observed with 2β (87%), 3β (95%), 4β (ONPG; 93%), 5β (97%), and 6β (90%). In contrast, the meta methoxy groups in compound 7β (76%) did not enhance binding strength compared to 1β. Further, para-substituted functional groups displayed variable inhibitory potencies relative to 1β, with enhancements observed in 8β (86%) and 90 (86%), no significant effect observed in 11β (78%) or 11β-thio (72%), and reduction observed in 1013 (65%). Thus, ortho-substituted phenyl β-galactosides generally outperformed other simple phenyl galactosides.
Complex heterocyclic galactosides, such as coumarins 12β (85%) and 14β (89%), which differ only by a methyl group, displayed significant inhibitory potencies against FmlHLD, while the related galactoside 13β (50%) displayed reduced inhibitory activity likely because of its fluoro-substituents (
Moreover, the tested GalNAc-derived compounds possessed significantly higher inhibitory potency compared to their Gal-derived counterparts, as observed with 4β-NAc (87%) relative to 4β (31%) when tested for inhibition at 10 μM (Table 2). These results suggested that the N-acetyl group together with other functional groups contributes to binding by targeting distinct components of the binding pocket of FmlH. In contrast, galactosides with α-linkages (28-30α) or disaccharides with aglycone moieties (33-35) were generally poor inhibitors of FmlH, except for 11α-NAc (82%) (Table 2).
Consistent with the above-mentioned SAR, the ortho-biphenyl galactoside 28β (91%) was more potent than the meta 31β (57%) or para 32β (30%) analogs (Table 2). This inhibition was enhanced with the addition of a carboxylate group at the meta position on the biphenyl B-ring (29β), intended to target the pocket formed by N140 and R142, and which resulted in a compound (29β) that exhibited greater inhibition (99%) compared to 28β when tested at 100 μM (Table 2). This difference in activity was further highlighted when these compounds were tested for inhibition at 10 μM and 1 μM (Table 2). Importantly, 30β, the methyl ester of 29β (87%), tested at 100 μM results in a reduction in binding, suggesting that the negative charge of the carboxylic acid likely mediates a critical electrostatic interaction with R142 of FmlHLD. Finally, the GalNAc version of 2913 was synthesized (Example 15) to increase its binding affinity and found that 29β-NAc (93%) had significant improvement in activity over 29β (75%) when tested at 10 μM. Final evaluation of the highest performing galactosides in the ELISA-based competition assay at a concentration of 10 μM and 1 μM allowed for a clearer ranking of compounds, where 29β-NAc clearly stood out as the most potent (Table 2).
Bio-layer interferometry (BLI) was used to quantitate the binding affinity of select galactosides to FmlH.
Bio-layer interferometry (BLI) protocol: Streptavidin (SA) pins were first dipped in a baseline in PBS (pH 7.4) for 120 s, followed by loading of 5-10 μg/mL biotinylated ser-TF (Toronto Research Chemicals) in PBS for 300 s, quenching by 10 μg/mL biocytin in PBS for 240 s, and another baseline step in PBS for 120 s. Thereafter, pins were dipped in PBS for 120 s and transferred to protein samples (varying concentration of FmlHLD or fixed concentration of FmlHLD with varying concentration of galactoside compounds) for association for 300-600 s. Equilibrium binding response values were used to determine the affinity of interaction between FmlHLD and immobilized ser-TF or between FmlHLD and galactosides in solution.
Steady-state analysis of binding responses from incubation of biotinylated serine-linked TF (ser-TF) immobilized on Streptavidin pins with varied titrations of FmlHLD in solution revealed a dissociation constant, KD, of 15.0±0.8 μM (
To elucidate the molecular basis for galactoside inhibition of FmlH, co-crystal structures of FmlHLD bound to 4β, 5β, 20β, and 29β-NAc were determined (
Crystals of apo FmlHLD in 10 mM Hepes pH 7.5, 50 mM NaCl were grown by mixing 2 μl protein (6 mg/ml) with 2 μl of mother liquor (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 28% PEG 3350) and equilibrated against 1 ml mother liquor in the reservoir. Co-crystals of FmlHLD bound to TF or galactosides 4β (in space group P 2 21 21), 5β, and 20β were grown by mixing 2 μl protein (6 mg/ml) in the presence of 5 mM compound with 2 μl of mother liquor (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 32% PEG 3350). These crystals were transferred into cryoprotectant (0.2 M ammonium sulfate, 0.1M NaCl, 0.1 M MES (pH 5.6), 35% PEG 4000, 10% glycerol) and then flash frozen in liquid nitrogen. Co-crystals of FmlHLD bound to galactoside 29β-NAc were grown by mixing 2 μl protein (10 mg/ml) with 2 μl of mother liquor (0.7 M LiSO4, 2% PEG8000). These crystals were transferred into cryoprotectant (1 M LiSO4, 10% PEG8000, 25% glycerol). Diffraction data for FmlHLD TF, 4β (in space group C 1 2 1), and 29β-NAc structures were collected at 100 K at an in-house facility equipped with a rotating anode Rigaku MicroMax 007 generator, a Rayonix Marmux X-ray source (Evanston, Ill.), and a Mar345 image plate detector. Diffraction data for FmlHLD apo, 4β, 5β, and 20β structures were collected at 100 K at the ALS Beamline 4.2.2. Data were indexed and integrated in iMosflm [43], XDS [44], or HKL2000 and scaled by Scala [45]. The phase problem was solved by molecular replacement (MR) using Phaser-MR in PHENIX [46] with FimHLD from PDB ID 3MCY. Several rounds of refinements were performed in PHENIX to improve the final models.
The galactosides examined (4β, 5β, and 20β, and 29β) share a common aglycone motif consisting of a phenyl ring with an ortho-substituted functional group. As predicted from computational studies, the sugar portion of these galactosides all resided within the cleft of the binding pocket. The phenyl groups directly attached to the sugar portion of all four compounds lie along the same three-dimensional plane. In this nearly-identical conformation, the phenyl ring was found to be oriented perpendicularly to the side chain of residue Y46, revealing edge-to-face π-stacking which likely contributed to the enhanced affinity observed for all β-galactosides. For 4β, 5β, and 20β, the ortho-substituents pointed toward R142 but were too distant (>7 Å) for direct interaction and instead formed H-bonds with water molecules that in turn interacted with residues K132 and R142 (
In contrast, the biphenyl scaffold of 29β-NAc presented the carboxylic acid to engage in a direct charge-charge interaction with the guanidinium side chain of R142 (
To confirm the importance of the binding pocket explored extensively in previous examples, a target mutation (K132Q) was made to produce a putative binding-null mutant FmlH. The ability of K132Q-FmlH to bind ds-BSM in vitro was compared to native FmlH in an ELISA assay using the procedure described in Example 18. Data is summarized in
To assess therapeutic efficacy, galactosides were evaluated for their ability to reduce bacterial burdens in the urinary tracts of C3H/HeN mice during chronic UTI. 7-8 week old female C3H/HeN mice were obtained from Envigo (Indianapolis, Ind.). Mice were anesthetized and inoculated via transurethral catheterization with 50 μl of CFT073 bacterial suspension (˜1-2*108 CFU in total) in phosphate-buffered saline (PBS). Chronic cystitis in C3H/HeN mice was defined as urine titers of >104 CFU/ml lasting at least two to four weeks, as well as bladder inflammation and edema at euthanasia [30]. Further, C3H/HeN mice are genetically predisposed to vesicoureteral reflux (retrograde flow of urine from the bladder to the kidneys), which can lead to bacterial colonization of the kidneys, renal abscess formation, scarring and atrophy {Murawski, 2010 #4776}. Accordingly, high levels of CFT073 kidney colonization were also observed in control (vehicle-treated) animals.
Mice meeting the criteria for chronic cystitis (high titers of bacteriuria (>104 CFU/ml) and edematous and inflamed bladders after 2 weeks) were then transurethrally inoculated either with 50 mg/kg compound or vehicle control (10% DMSO). Compounds tested were: 29β-NAc (50 mg/kg), mannoside 4Z269 (a type 1 pilus adhesin FimH inhibitor, 50 mg/kg), or 29β-NAc+4Z269. Mice were sacrificed 6 hours post treatment and bacteria colonizing the bladder or kidney were plated for quantification. Data are presented in
Treatment of mice experiencing chronic cystitis with 50 mg/kg 29β-NAc through transurethral delivery 6 hours prior to euthanasia led to a significant reduction of CFT073 in the bladders of infected mice relative to vehicle control (
The relevance of FmlH and FmlH-targeting galactosides in human UTI was assessed by performing immunofluorescence analysis of wild-type (WT) FmlHLD or mutant (K132Q) FmlHLD binding to human bladder and non-malignant kidney biopsied tissue in the presence or absence of 29β-NAc. The mutant FmlHLD(K132Q) was shown to have reduced inhibition in previous Example 25.
Frozen, de-identified human bladder and kidney sections were obtained from the Tissue Procurement Core and stored stably at −80° C. These tissue section slides were removed from the freezer and allowed to thaw at room temperature for 10-20 min. After applying a hydrophobic barrier pen around the tissue, slides were rehydrated in 200 μl buffer (5% BSA, 0.2% Triton X-100 in PBS) for 10 min. Buffer was gently aspirated and slides were blocked for 1 hr at room temperature with 200 μl buffer. Thereafter, buffer was gently aspirated and slides were incubated with 200 μl sample overnight at 4° C. Samples diluted in buffer included 50 μg/ml FmlHLD wild-type (WT), 50 μg/ml FmlHLD K132Q, and 50 μg/ml FmlHLD WT incubated with 100 μM 29β-NAc. Samples were gently aspirated and slides were washed three times in buffer for 5 min each. Next, slides were incubated with a mouse anti-FmlH polyclonal antibody (1:500 dilution in buffer) for 1 hr at room temperature. Slides were washed again three times in buffer and then incubated in the dark with donkey anti-mouse IgG, Alexa Fluor 594 and Wheat Germ Agglutinin, Alexa Fluor 633 (each 1:500 dilution in buffer) for 1 hr at room temperature. Slides were washed once with buffer and then incubated in the dark with DAPI (1:1000 dilution in buffer) for 5 min at room temperature. After washing twice with buffer, coverslips were mounted using 80 μl mounting media. Slides were loaded onto a Zeiss LSM 880 Confocal Laser Scanning Microscope (Carl Zeiss Inc., Thornwood, N.Y.) equipped with a diode 405-430 laser, a HeNe 543 laser, and a HeNe 633 laser. Images were acquired with a 20×, 0.8 numerical aperture Zeiss Plan Apochromat objective using ZEN 2 imaging software.
Representative images are depicted in
The Ugi reaction was used to combine an aldehyde, amine, carboxylic acid, and isocyanide into a galactoside containing an “N-(tert-butyl)-2-(N-methylacetamido) acetamide” linkage as described in the General Scheme outlined below.
Using the following reactants, specific quinoline containing galactosides with the “N-(tert-butyl)-2-(N-methylacetamido) acetamide” linkage were synthesized: β-GalNAc-amine (2R,3R,4R,5R,6R)-5-acetamido-2-(acetoxymethyl)-6-aminotetrahydro-2H-pyran-3,4-diyl diacetate), f3-Gal amine ((2R,3S,4S,5R,6R)-2-(acetoxymethyl)-6-aminotetrahydro-2H-pyran-3,4,5-triyl triacetate), carboxylic acid (quinoline-8-carboxylic acid), and isocyanide (2-isocyano-2-methylpropane). The specific reaction protocols are diagrammed and described below:
(A) General Ugi Reaction Procedure with β-GalNAc/β-Gal Amine
Into a solution of aldehyde (38 mg, 0.650 mmol) in methanol (5 mL), β-GalNAc or β-Gal amine (400 mg, 0.650 mmol) was added and stirred for 5 minutes at room temperature. Then, carboxylic acid (200 mg, 0.650 mmol), and isocyanide (130 μL, 0.650 mmol) were added. The reaction was continually stirred until no noticeable starting reagents were visualized using TLC. Upon completion of the reaction, methanol was evaporated under reduced pressure and the crude compound subjected to flash column chromatography (EtOAc/Hexanes) to yield pure compound ((2R,3R,4R,5R,6R)-5-acetamido-2-(acetoxymethyl)-6-(N-(2-(tert-butylamino)-2-oxoethyl)quinoline-8-carboxamido)tetrahydro-2H-pyran-3,4-diyl diacetate and (2R,3S,4S,5R,6R)-2-(acetoxymethyl)-6-(N-(2-(tert-butylamino)-2-oxoethyl)quinoline-8-carboxamido)tetrahydro-2H-pyran-3,4,5-triyl triacetate).
(B) General Procedure for Deacetylation
33% Wt. Methylamine in absolute ethanol solution (5 mL) was added to (2R, 3 S,4 S,5R, 6R)-2-(acetoxymethyl)-6-(N-(2-(tert-butylamino)-2-oxoethyl)quinoline-8-carboxamido)tetrahydro-2H-pyran-3,4,5-triyl triacetate (100 mg, 0.163 mmol). The reaction solution was stirred at the same temperature (0.5-1 h) until TLC indicated complete disappearance of staring material. Complete evaporation of the solvent provided the pure compound.
Compound was isolated as a white solid, 55 mg in 69% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 8.87-8.93 (m, 1H), 8.53 (d, J=7.8 Hz, 1H), 8.12 (d, J=8.6 Hz, 1H), 7.57-7.77 (m, 4H), 5.74 (d, J=9.8 Hz, 1H), 4.08 (d, J=16.4 Hz, 2H), 3.85 (br. s., 2H), 3.77 (d, J=10.6 Hz, 1H), 3.42-3.52 (m, 3H), 3.34 (br. s., 1H), 3.14 (d, J=9.8 Hz, 1H), 1.83 (br. s., 3H), 1.32 (s, 9H); LCMS (ESI): found [M+H]+, 489.4.
Compound was isolated as a white solid, 51 mg in 58% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 8.88 (d, J=3.9 Hz, 1H), 8.47 (d, J=8.6 Hz, 1H), 8.08 (d, J=9.4 Hz, 1H), 7.68 (br. s., 1H), 7.62 (dd, J=8.2, 4.3 Hz, 2H), 5.54 (d, J=9.0 Hz, 1H), 4.05 (d, J=8.6 Hz, 3H), 3.68 (br. s., 3H), 3.36-3.61 (m, 6H), 2.93 (dd, J=9.4, 2.7 Hz, 1H), 1.33 (s, 9H); LCMS (ESI): found [M+H]+, 448.4.
Using the reaction scheme described below, a variety of substituted biaryl galactosides and bi-aryl N-acetyl galactosides were synthesized. The detailed experimental protocols for each method illustrated (Method A, Method B, Suzuki reaction, Deacetylation) are outlined below.
General Procedure for Glycosylation (Method A).
1N aqueous NaOH solution (1 mL) was added into a solution of 2-acetamido-3,4,6,-tri-O-acetyl-1-chloro-1,2-dideoxy-α-D-galactopyranose (100 mg, 0.273 mmol), tetrabutylammonium bromide (88 mg, 0.273 mmol) and 2-bromo phenol (79 mg, 0.546 mmol) in dichloromethane (2 mL) at room temperature. The reaction solution was stirred at the same temperature until the TLC indicated complete disappearance of chloride. The reaction mass was then diluted with dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, giving rise to the 129 mg of Product A ((2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(2-bromophenoxy)tetrahydro-2H-ran-3,4-diyl diacetate) as a white solid.
General Procedure for Glycosylation (Method B)
1N aqueous NaOH solution (1 mL) was added into a solution of (2R,3S,4S,5R,6R)-2-(acetoxymethyl)-6-bromotetrahydro-2H-pyran-3,4,5-triyl triacetate (200 mg, 0.487 mmol), benzyltriethylammonium chloride (111 mg, 0.0.487 mmol) and 2-bromo phenol (79 mg, 0.975 mmol) in chloroform (2 mL) at room temperature. The reaction solution was stirred at 60° C. temperature until the TLC indicated complete disappearance of starting material. The reaction solution was then cooled and diluted with the dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, giving rise to the 200 mg of Product B ((2R,3S,4S,5R,6S)-2-(acetoxymethyl)-6-(2-bromophenoxy)tetrahydro-2H-pyran-3,4,5-triyl triacetate) as a white solid.
General Procedure for Suzuki Reaction
Under nitrogen atmosphere (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(2-bromophenoxy) tetrahydro-2H-pyran-3,4-diyl diacetate (100 mg, 0.199 mmol), 3-(N-methyl amino carbonyl) phenyl boronic acid (78 mg, 0.298 mmol), Pd(PPh3)4 (23 mg, 0.0199 mmol) and Cesium carbonate (211 mg, 0.597 mmol) were added to a reaction vial followed by a dioxane/water mixture (5:1, 3.6 mL), before heating the reaction solution to 80° C. The reaction mixture was stirred at 80° C. until TLC indicated complete disappearance of staring material (1 h). The reaction solution was then cooled and diluted with dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by column chromatography with hexane/ethyl acetate combinations as eluent, giving rise to the desired product.
General Procedure for Deacetylation
33% Wt. Methylamine in absolute ethanol solution was added into (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(quinolin-8-yloxy)tetrahydro-2H-pyran-3,4-diyl diacetate (50 mg, 0.105 mmol). The reaction solution was stirred at the same temperature (0.5-1 h) until TLC indicated complete disappearance of staring material. Complete evaporation of the solvent provided the pure compound.
These procedures were repeated using a variety of substituted reactants to produce Compounds 1189, 2021 and 2028-2050. The chemical identity and spectral characteristics for each compound are described in the following examples (Examples 31-53).
Compound was isolated as a white solid, 62 mg in 73% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.93 (d, J=3.91 Hz, 1H), 7.73 (t, J=5.09 Hz, 1H), 7.65 (t, J=5.87 Hz, 1H), 7.44-7.50 (m, 1H), 7.36 (d, J=4.70 Hz, 3H), 7.09-7.16 (m, 1H), 5.07-5.13 (m, 1H), 4.10-4.17 (m, 1H), 3.74-3.93 (m, 3H), 3.64-3.73 (m, 2H), 2.96 (d, J=5.09 Hz, 3H), 1.64 (d, J=4.70 Hz, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.03, 156.12, 140.03, 135.80, 133.90, 131.92, 130.44, 129.54, 126.83, 123.96, 116.96, 101.53, 77.41, 73.27, 69.79, 62.64, 54.23, 27.12, 22.82; LCMS (ESI): found [M+Na]+, 453.3.
Compound was isolated as a white solid, 30 mg in 70% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.25-7.36 (m, 3H), 7.10-7.23 (m, 2H), 6.83-7.09 (m, 4H), 5.06 (d, J=8.6 Hz, 1H), 4.11 (dd, J=10.4, 8.8 Hz, 1H), 3.88 (d, J=3.1 Hz, 1H), 3.74-3.86 (m, 2H), 3.60-3.72 (m, 2H), 1.73 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.31, 158.05, 156.13, 140.97, 132.87, 131.78, 129.79, 123.74, 122.35, 117.86, 117.09, 115.08, 101.44, 77.35, 73.63, 69.81, 62.63, 54.36, 22.96; LCMS (ESI): found [M+Na]+, 412.3.
Compound was isolated as a white solid, 32 mg in 85% yield; 41 NMR (400 MHz, CD3OD) δ ppm 7.24-7.41 (m, 5H), 7.20 (dd, J=10.4, 1.8 Hz, 1H), 7.07-7.14 (m, 1H), 7.00-7.07 (m, 1H), 5.06 (d, J=8.2 Hz, 1H), 4.10-4.19 (m, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.75-3.87 (m, 2H), 3.67-3.72 (m, 1H), 3.61 (dd, J=11.0, 3.1 Hz, 1H), 1.72 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.95, 165.19, 162.76156.03, 141.98, 131.78, 131.54, 130.82, 130.74, 130.47, 126.70, 126.67, 123.85, 117.70, 117.48, 116.99, 114.82, 114.61, 101.42, 77.41, 73.74, 69.81, 62.62, 54.12, 22.83; LCMS (ESI): found [M+Na]+, 414.3.
Compound was isolated as a white solid, 20 mg in 87% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.34-7.39 (m, 1H), 7.22-7.33 (m, 4H), 7.00-7.12 (m, 3H), 6.86 (d, J=8.2 Hz, 1H), 5.03 (d, J=8.2 Hz, 1H), 4.12 (t, J=9.8 Hz, 1H), 3.85-3.91 (m, 1H), 3.83 (s, 3H), 3.74-3.81 (m, 1H), 3.64-3.71 (m, 1H), 3.60 (dd, J=10.6, 2.3 Hz, 1H), 2.55 (s, 1H), 1.65 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.07, 160.81, 156.24, 141.06, 132.95, 131.84, 129.96, 123.85, 123.23, 117.29, 116.47, 113.80, 101.85, 77.40, 73.79, 69.82, 62.64, 55.83, 54.25, 22.80; LCMS (ESI): found [M+Na]+, 426.3.
Compound was isolated as a white solid, 35 mg in 91% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.33 (s, 1H), 8.19 (d, J=8.2 Hz, 1H), 7.87 (d, J=7.8 Hz, 1H), 7.62 (t, J=8.0 Hz, 1H), 7.35-7.41 (m, 3H), 7.13-7.18 (m, 1H), 5.07 (d, J=8.6 Hz, 1H), 4.08-4.14 (m, 1H), 3.89 (d, J=2.7 Hz, 1H), 3.75-3.87 (m, 3H), 3.67-3.73 (m, 1H), 3.61 (dd, J=10.6, 3.1 Hz, 1H), 2.55 (s, 1H), 1.63 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.74, 156.05, 149.62, 141.30, 137.07, 131.66, 131.20, 130.38, 125.60, 124.06, 122.87, 117.01, 101.51, 77.43, 73.50, 69.77, 62.62, 54.04, 22.77; LCMS (ESI): found [M+Na]+, 441.3.
Compound was isolated as a white solid, 29 mg in 86% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.55-7.60 (m, 2H), 7.47 (t, J=7.8 Hz, 1H), 7.29-7.39 (m, 4H), 7.08-7.14 (m, 1H), 5.17 (d, J=8.2 Hz, 1H), 3.98-4.06 (m, 1H), 3.89 (d, J=2.7 Hz, 1H), 3.73-3.84 (m, 2H), 3.65-3.73 (m, 2H), 3.12 (d, J=5.1 Hz, 6H), 1.74 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.94, 156.03, 140.15, 136.96, 132.31, 131.84, 130.44, 129.66, 129.41, 126.62, 123.90, 116.88, 101.05, 77.35, 73.42, 69.77, 62.63, 54.49, 40.58, 35.88, 23.01; LCMS (ESI): found [M+Na]+, 445.3.
Compound was isolated as a white solid, 13 mg in 85% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.99 (s, 1H), 7.79 (d, J=7.83 Hz, 1H), 7.68 (d, J=7.83 Hz, 1H), 7.45-7.50 (m, 1H), 7.31-7.39 (m, 4H), 7.12 (t, J=6.85 Hz, 1H), 5.11 (d, J=8.22 Hz, 1H), 4.10 (dd, J=8.80, 10.37 Hz, 1H), 3.89 (d, J=3.13 Hz, 1H), 3.75-3.87 (m, 3H), 3.64-3.72 (m, 3H), 1.64 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.01, 172.99, 156.13, 140.02, 135.11, 134.37, 131.91, 130.44, 129.96, 129.41, 127.24, 123.97, 117.00, 101.50, 77.41, 73.26, 62.63, 54.32, 22.82; LCMS (ESI): found [M+Na]+, 439.3.
Compound was isolated as a white solid, 16 mg in 84% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.77-7.82 (m, 2H), 7.67 (d, J=9.00 Hz, 1H), 7.53-7.59 (m, 1H), 7.31-7.40 (m, 3H), 7.10-7.17 (m, 1H), 5.07 (d, J=8.22 Hz, 1H), 4.10-4.18 (m, 1H), 3.90 (d, J=3.13 Hz, 1H), 3.74-3.87 (m, 2H), 3.67-3.73 (m, 1H), 3.61 (dd, J=2.54, 10.76 Hz, 1H), 1.70 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.77, 155.97, 141.05, 135.62, 134.28, 131.72, 131.03, 130.36, 123.99, 120.08, 116.88, 113.37, 101.38, 77.42, 73.58, 69.77, 62.61, 54.01, 22.87; LCMS (ESI): found [M+Na]+, 421.3.
Compound was isolated as a white solid, 14 mg in 86% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.78 (d, J=7.4 Hz, 1H), 7.68 (s, 1H), 7.53-7.63 (m, 2H), 7.36-7.39 (m, 2H), 7.30-7.36 (m, 1H), 7.11-7.17 (m, 1H), 5.07 (d, J=8.6 Hz, 1H), 4.11 (dd, J=10.4, 8.8 Hz, 1H), 3.89 (br. s., 1H), 3.75-3.86 (m, 2H), 3.66-3.73 (m, 1H), 3.61 (dd, J=10.8, 3.3 Hz, 1H), 1.63 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.39, 156.11, 140.70, 135.02, 131.74, 131.33, 130.81, 129.92, 126.95, 124.75, 117.02, 101.53, 77.44, 73.72, 69.78, 62.62, 54.13, 22.73; LCMS (ESI): found [M+Na]+, 464.3.
Compound was isolated as a white solid, 34 g in 96% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.40 (s, 1H), 7.36 (s, 1H), 7.32 (d, J=5.87 Hz, 3H), 7.22-7.29 (m, 2H), 7.07-7.12 (m, 1H), 5.12 (d, J=8.61 Hz, 1H), 4.11 (dd, J=8.61, 10.56 Hz, 1H), 3.89 (d, J=3.13 Hz, 1H), 3.73-3.85 (m, 2H), 3.63-3.71 (m, 2H), 3.03 (s, 3H), 1.73 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.06, 155.99, 140.93, 139.06, 131.97, 130.27, 127.25, 123.82, 120.68, 116.69, 101.09, 77.35, 73.46, 62.60, 54.24, 39.58, 23.04; LCMS (ESI): found [M+Na]+, 489.2.
Compound was isolated as a white solid, 49 mg in 65% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.03 (dd, J=1.96, 8.61 Hz, 1H), 7.78 (d, J=2.35 Hz, 1H), 7.29-7.36 (m, 2H), 7.15 (d, J=7.43 Hz, 1H), 7.03-7.10 (m, 2H), 4.95 (d, J=8.22 Hz, 1H), 3.96 (dd, J=8.80, 10.37 Hz, 1H), 3.83-3.88 (m, 1H), 3.82 (s, 3H), 3.73-3.80 (m, 1H), 3.62-3.67 (m, 1H), 3.58 (dd, J=3.13, 10.56 Hz, 1H), 1.62 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.77, 170.06, 162.78, 156.91, 134.12, 132.47, 132.33, 130.16, 129.56, 129.31, 123.58, 123.31, 116.78, 112.06, 111.59, 101.61, 77.29, 73.49, 69.80, 62.62, 56.50, 54.20, 22.87; LCMS (ESI): found [M+Na]+, 470.3.
Compound was isolated as a white solid, 36 mg in 91% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.02 (dd, J=8.6, 2.0 Hz, 1H), 7.77 (d, J=2.0 Hz, 1H), 7.29-7.37 (m, 2H), 7.14 (d, J=7.4 Hz, 1H), 7.04-7.11 (m, 2H), 4.94 (d, J=8.2 Hz, 1H), 4.34 (q, J=7.0 Hz, 2H), 3.96 (dd, J=10.4, 8.8 Hz, 1H), 3.83-3.87 (m, 2H), 3.82 (s, 3H), 3.74-3.80 (m, 1H), 3.62-3.67 (m, 1H), 3.57 (dd, J=11.0, 3.1 Hz, 1H), 1.60 (s, 3H), 1.37 (t, J=7.2 Hz, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.08, 168.72, 163.27, 157.34, 134.21, 132.57, 130.64, 129.92, 123.74, 117.21, 112.52, 102.09, 77.72, 73.94, 70.20, 63.04, 62.39, 56.93, 54.5623.30, 15.26; LCMS (ESI): found [M+Na]+, 498.3.
Compound was isolated as a white solid, 14 mg in 60% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.10 (s, 1H), 7.90 (d, J=7.8 Hz, 1H), 7.81 (d, J=7.8 Hz, 1H), 7.62-7.69 (m, 1H), 7.34-7.42 (m, 3H), 7.12-7.18 (m, 1H), 5.14 (d, J=8.2 Hz, 1H), 4.06 (dd, J=10.6, 8.6 Hz, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.75-3.87 (m, 3H), 3.63-3.71 (m, 2H), 3.24 (s, 2H), 1.68 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.81, 156.05, 141.91, 141.01, 136.05, 131.77, 131.06, 130.44, 129.69, 126.80, 124.10, 116.95, 101.46, 77.44, 73.41, 69.77, 62.61, 54.27, 44.65, 22.93; LCMS (ESI): found [M+Na]+, 474.3.
Compound was isolated as a white solid, 38 mg in 96% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.63 (t, J=1.8 Hz, 1H), 7.56 (d, J=9.0 Hz, 1H), 7.28-7.36 (m, 5H), 7.23 (s, 1H), 7.09 (td, J=7.2, 1.6 Hz, 1H), 5.11 (d, J=8.6 Hz, 1H), 4.13 (dd, J=10.6, 8.6 Hz, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.74-3.85 (m, 2H), 3.65-3.70 (m, 2H), 2.16 (s, 3H), 1.70 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.17, 171.86, 155.99, 140.15, 139.38, 132.16, 131.78, 130.08, 129.58, 126.81, 132.83, 123.23, 120.35, 116.84, 101.05, 77.32, 73.37, 69.80, 62.63, 54.32, 24.01, 23.02; LCMS (ESI): found [M+Na]+, 453.3.
Compound was isolated as a white solid, 33 mg in 87% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.53 (s, 1H), 7.27-7.40 (m, 6H), 7.06-7.12 (m, 1H), 4.97 (d, J=8.61 Hz, 1H), 4.63 (s, 2H), 4.18 (dd, J=8.61, 10.56 Hz, 1H), 3.88 (d, J=3.52 Hz, 1H), 3.82-3.86 (m, 1H), 3.76-3.81 (m, 1H), 3.66-3.71 (m, 1H), 3.59 (dd, J=3.13, 10.56 Hz, 1H), 1.54 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.24, 156.32, 142.22, 132.65, 131.80, 130.32, 129.99, 129.65, 129.33, 127.18, 123.90, 117.10, 101.91, 77.42, 73.47, 69.82, 65.84, 62.68, 54.09, 22.70; LCMS (ESI): found [M+Na]+, 426.3.
Compound was isolated as a white solid, 34 mg in 87% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.53 (br. s., 1H), 7.41 (d, J=8.61 Hz, 1H), 7.27-7.35 (m, 4H), 7.16 (d, J=7.83 Hz, 1H), 7.06-7.11 (m, 1H), 5.11 (d, J=8.61 Hz, 1H), 4.09 (dd, J=8.61, 10.56 Hz, 1H), 3.88 (d, J=3.13 Hz, 1H), 3.77-3.85 (m, 2H), 3.74 (s, 3H), 3.68 (dd, J=3.13, 10.56 Hz, 2H), 1.70 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.17, 156.01, 140.27, 132.46, 131.85, 130.00, 129.57, 125.88, 123.39, 116.89, 101.08, 77.32, 73.34, 69.83, 62.61, 54.42, 52.67, 22.96; LCMS (ESI): found [M+Na]+, 469.2.
Compound was isolated as a white solid, 21 mg in 91% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.14 (dd, J=7.4, 1.2 Hz, 1H), 7.03 (t, J=6.8 Hz, 1H), 6.86 (d, J=9.0 Hz, 1H), 6.73 (dd, J=8.8, 2.9 Hz, 1H), 6.60 (d, J=3.1 Hz, 1H), 4.95 (d, J=8.2 Hz, 1H), 4.00 (dd, J=10.6, 8.6 Hz, 1H), 3.86 (d, J=2.7 Hz, 1H), 3.80-3.84 (m, 1H), 3.74-3.79 (m, 1H), 3.66 (s, 3H), 3.61-3.64 (m, 1H), 3.56-3.61 (m, 2H), 1.73 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.15, 156.75, 152.19, 151.93, 132.55, 130.44, 129.68, 123.21, 119.49, 117.10, 115.98, 114.77, 101.70, 77.25, 73.62, 69.80, 62.61, 57.44, 54.32, 23.02; LCMS (ESI): found [M+Na]+, 442.3.
Compound was isolated as a white solid, 13 mg in 82% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.25-7.34 (m, 3H), 7.20 (d, J=7.4 Hz, 1H), 7.14 (d, J=2.7 Hz, 1H), 7.02-7.10 (m, 2H), 5.01 (d, J=8.2 Hz, 1H), 3.93-4.01 (m, 1H), 3.83-3.89 (m, 1H), 3.79-3.83 (m, 1H), 3.74 (s, 1H), 3.64 (t, J=6.1 Hz, 2H), 3.23 (s, 3H), 1.72 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.87, 156.47, 143.93, 132.69, 128.77, 126.45, 123.27, 116.95, 113.58, 101.39, 77.30, 73.34, 69.75, 62.59, 56.79, 54.31, 37.38, 23.05; LCMS (ESI): found [M+Na]+, 520.2.
Compound was isolated as a white solid, 30 mg in 94% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.46-7.51 (m, 2H), 7.32-7.38 (m, 2H), 7.27-7.32 (m, 1H), 7.10-7.16 (m, 1H), 5.15 (d, J=8.22 Hz, 1H), 4.05 (dd, J=8.41, 10.76 Hz, 1H), 3.89 (d, J=3.13 Hz, 1H), 3.75-3.84 (m, 2H), 3.66-3.73 (m, 2H), 3.29 (s, 3H), 1.71 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.98, 156.04, 150.72, 141.82, 131.81, 131.30, 130.72, 129.89, 124.60, 124.02, 121.79, 117.10, 101.35, 77.43, 73.27, 69.79, 62.61, 54.47, 37.77, 22.96; LCMS (ESI): found [M+Na]+, 490.3.
Compound was isolated as a white solid, 34 mg in 97% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.19 (s, 1H), 7.75 (dd, J=13.5, 8.0 Hz, 2H), 7.49 (t, J=7.6 Hz, 1H), 7.32-7.40 (m, 3H), 7.11 (ddd, J=7.7, 5.2, 3.1 Hz, 1H), 5.05 (d, J=7.8 Hz, 1H), 3.90 (d, J=3.5 Hz, 1H), 3.67-3.80 (m, 4H), 3.61 (d, J=3.5 Hz, 1H), 3.58 (d, J=3.5 Hz, 1H), 2.94 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 170.95, 155.94, 140.12, 135.01, 133.79, 131.78, 130.37, 129.92, 126.89, 123.76, 116.84, 102.98, 77.17, 75.33, 72.62, 70.38, 62.54, 27.14; LCMS (ESI): found [M+Na]+, 412.3.
Compound was isolated as a white solid, 33 mg in 93% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.54 (s, 1H), 7.27-7.39 (m, 5H), 7.19-7.26 (m, 1H), 7.06-7.12 (m, 1H), 5.06 (d, J=7.8 Hz, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.72-3.77 (m, 2H), 3.66-3.72 (m, 2H), 3.57 (dd, J=9.6, 3.3 Hz, 1H), 3.01 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 153.37, 141.09, 138.77, 131.72, 129.98, 127.19, 123.71, 123.36, 120.63, 116.29, 101.96, 76.91, 75.02, 72.17, 70.15, 62.2739.16; LCMS (ESI): found [M+Na]+, 448.2.
Compound was isolated as a white solid, 42 mg in 89% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.64 (d, J=2.0 Hz, 1H), 8.59-8.61 (m, 1H), 8.55 (s, 1H), 7.37-7.49 (m, 3H), 7.16-7.22 (m, 1H), 5.05 (d, J=7.4 Hz, 1H), 3.91 (d, J=3.5 Hz, 1H), 3.75-3.83 (m, 2H), 3.67-3.74 (m, 2H), 3.60 (dd, J=9.8, 3.5 Hz, 1H), 2.98 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 168.34, 156.06, 149.96, 141.95, 136.66, 135.53, 131.66, 131.59, 129.82, 127.91, 124.16, 121.76, 117.42, 103.41, 77.28, 75.27, 72.54, 70.36, 62.54, 27.27; LCMS (ESI): found [M+Na]+, 457.2.
Compound was isolated as a white solid, 62 mg in 88% yield; 1H NMR (400 MHz, CD3OD) δ ppm; 8.73 (s, 2H), 8.58 (s, 1H), 7.37-7.46 (m, 4H), 7.15-7.21 (m, 1H), 5.01 (d, J=7.4 Hz, 1H), 3.89 (d, J=3.5 Hz, 1H), 3.74-3.83 (m, 2H), 3.66-3.72 (m, 2H), 3.56 (dd, J=9.6, 3.3 Hz, 1H); 13C NMR (100 MHz, CD3OD) δ ppm 167.88, 156.03, 149.75, 142.10, 137.57, 133.60, 131.57, 129.73, 124.13, 123.66, 117.52, 103.33, 77.27, 75.31, 72.33, 70.3562.51; LCMS (ESI): found [M+Na]+, 444.3.
General Procedure for Glycosylation.
1N aqueous NaOH solution (1 mL) was added into a solution of 2-Acetamido-3,4,6,-tri-O-acetyl-1-chloro-1,2-dideoxy-α-D-galactopyranose (100 mg, 0.273 mmol), tetrabutylammonium bromide (88 mg, 0.273 mmol) and 8-hydroxy quinoline (79 mg, 0.546 mmol) in dichloromethane (2 mL) at room temperature. The reaction solution was stirred at the same temperature until the TLC indicated complete disappearance of chloride. The reaction mass was then diluted with dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, giving rise to the 129 mg of (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(quinolin-8-yloxy)tetrahydro-2H-pyran-3,4-diyl diacetate as a white solid.
General Procedure for Deacetylation
33% Wt. Methylamine in absolute ethanol solution was added into (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(quinolin-8-yloxy)tetrahydro-2H-pyran-3,4-diyl diacetate (50 mg, 0.105 mmol). The reaction solution was stirred at the same temperature (0.5-1 h) until TLC indicates complete disappearance of staring material. Complete evaporation of the solvent provided the pure compound.
These procedures were repeated using different quinolines/isoquinolines in place of 8-hydroxyquinoline to produce compounds 1179, 1192, 1180, 1184, 2002, 2003, and 2007. The chemical/spectral properties for each isolated compound are described in the following Examples 54-60.
Compound was isolated as a white solid, 24 mg in 65% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 8.20 (d, J=8.2 Hz, 1H), 7.55 (d, J=7.4 Hz, 1H), 7.34-7.47 (m, 3H), 5.29 (d, J=8.6 Hz, 1H), 4.07 (t, J=9.2 Hz, 1H), 3.73 (br. s., 2H), 3.42-3.63 (m, 4H), 2.65 (s, 3H), 1.80 (s, 3H); 13C NMR (100 MHz, DMSO-d6) δ ppm 171.17, 158.45, 136.81, 127.82, 126.17, 123.08, 121.92, 114.67, 109.98, 99.94, 76.01, 71.56, 67.79, 60.73, 53.07, 25.11, 23.44; LCMS (ESI): found [M+H]+, 363.3.
Compound was isolated as a white solid, 24 mg in 54% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.77 (d, J=3.5 Hz, 1H), 8.29 (d, J=7.8 Hz, 1H), 7.87 (d, J=9.0 Hz, 1H), 7.58 (br. s., 1H), 7.42 (dd, J=8.2, 4.3 Hz, 1H), 7.34 (d, J=7.8 Hz, 1H), 5.27 (d, J=8.2 Hz, 1H), 4.31 (t, J=9.4 Hz, 1H), 3.98 (br. s., 1H), 3.75-3.88 (m, 4H), 2.00 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.40, 160.31, 151.64, 150.11, 138.26, 130.68, 126.14, 121.37, 121.06, 111.80, 100.98, 77.32, 73.12, 69.62, 62.38, 54.24, 23.15; LCMS (ESI): found [M+H]+, 371.1.
Compound was isolated as a white solid, 22 mg in 68% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.71 (d, J=3.5 Hz, 1H), 8.28 (d, J=8.2 Hz, 1H), 7.95 (d, J=9.0 Hz, 1H), 7.46-7.54 (m, 3H), 5.22 (d, J=8.2 Hz, 1H), 4.24-4.31 (m, 1H), 3.95 (d, J=3.1 Hz, 1H), 3.84-3.90 (m, 1H), 3.76-3.83 (m, 3H), 2.00 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.43, 157.36, 149.55, 145.41, 137.77, 131.01, 130.64, 124.19, 122.95, 111.95, 101.27, 77.54, 73.09, 69.82, 62.75, 54.35, 23.17; LCMS (ESI): found [M+H]+, 371.4.
Compound was isolated as a white solid, 57 mg in 96% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 8.55 (d, J=4.3 Hz, 1H), 7.58 (d, J=8.2 Hz, 1H), 7.38 (t, J=8.2 Hz, 1H), 7.22-7.28 (m, 2H), 5.14 (d, J=8.2 Hz, 1H), 3.86 (t, J=9.2 Hz, 1H), 3.51-3.59 (m, 2H), 3.30-3.41 (m, 3H), 2.35 (br. s., 3H), 1.66 (s, 3H); 13C NMR (100 MHz, DMSO-d6) δ ppm 171.03, 153.33, 149.16, 144.73, 139.81, 129.28, 126.78, 122.69, 118.20, 114.48, 99.68, 75.90, 71.66, 67.62, 60.60, 53.23, 23.32, 18.86; LCMS (ESI): found [M+H]+, 363.3.
Compound was isolated as a white solid, 50 mg in 85% yield; 1H NMR (400 MHz, CD3OD) δ ppm 9.13 (s, 1H), 8.31 (d, J=5.5 Hz, 1H), 7.87 (d, J=8.6 Hz, 1H), 7.75 (d, J=5.5 Hz, 1H), 7.66 (s, 1H), 7.49 (d, J=9.0 Hz, 1H), 5.24 (d, J=8.6 Hz, 1H), 4.29 (t, J=9.6 Hz, 1H), 3.95 (br. s., 1H), 3.75-3.91 (m, 4H), 1.99 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.43, 158.08, 152.36, 141.50, 133.87, 131.34, 129.50, 125.70, 122.21, 11.36, 101.15, 77.56, 73.08, 69.82, 62.76, 54.26, 23.16; LCMS (ESI): found [M+H]+, 371.3.
Compound was isolated as a yellow solid, 95 mg in 86% yield; 1H NMR (400 MHz, CD3OD) δ ppm 9.90 (br. s., 1H), 8.67 (d, J=8.6 Hz, 1H), 8.50 (d, J=8.6 Hz, 1H), 8.33 (t, J=7.6 Hz, 1H), 8.14 (d, J=8.6 Hz, 1H), 7.93-8.00 (m, 2H), 7.81-7.89 (m, 1H), 5.41 (d, J=8.2 Hz, 1H), 4.54 (t, J=10.2 Hz, 1H), 4.01 (br. s., 1H), 3.78-3.91 (m, 4H), 2.01 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 176.11, 131.33, 129.67, 129.38, 124.31, 120.09, 103.16, 77.72, 72.38, 69.46, 62.66, 54.37, 23.55; LCMS (ESI): found [M+H]+, 399.3.
Compound was isolated as a white solid, 57 mg in 78% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 9.18 (s, 1H), 8.41 (d, J=5.9 Hz, 1H), 8.06 (d, J=9.0 Hz, 1H), 7.79 (d, J=9.0 Hz, 1H), 7.69 (d, J=5.5 Hz, 1H), 7.43 (s, 1H), 7.29 (d, J=10.2 Hz, 1H), 5.19 (d, J=8.2 Hz, 1H), 4.80 (d, J=6.3 Hz, 1H), 4.67-4.76 (m, 2H), 4.00-4.12 (m, 1H), 3.77 (br. s., 1H), 3.50-3.69 (m, 4H), 1.80 (s, 3H); 13C NMR (100 MHz, DMSO-d6) δ ppm 169.63, 158.41, 151.62, 143.23, 136.81, 129.61, 124.54, 120.09, 119.74, 108.56, 99.05, 75.80, 71.16, 67.46, 60.35, 51.64, 23.11; LCMS (ESI): found[M+H]+, 371.3.
The compounds synthesized in Examples 30 to 60 were tested in the ELISA-based competition assay for their ability to inhibit binding of FmlHLD to ds-BSM. The ELISA assay was performed using the protocol described in Example 18. All compounds were tested at 1 μM and a subset was additionally tested at 100 nM. Results are summarized in Table 4. Average percent inhibition (PI) values are reported for compounds tested at 1 μM (PI1) and 100 nM (PI0.1).
The synthesis of substituted bi-aryl galactosides and bi-aryl N-acetyl galactosides, compounds 29β-NAc, 2050, 2051, 2021, 2078, 2073, 2077, 2028, 2029, 2030, 2035, 2037, 2059, 2064, 2062, 3044, 2065, 2032, 1189, 2033, 2038, and 2043, was previously described in Example 29 and the chemical identity provided in Examples 30-52. Using the same procedures (which a minor modification to the deacetylation step described below), three new compounds (3029, 3044, and 3049) were synthesized. For ease of reference, the general synthesis scheme for these compounds is depicted in Scheme 1, below. Note that it is identical to the reaction schemes described in Example 29. Briefly, reaction steps “a” and “d” refer to glycosylation Methods A and B, respectively, as described in Example 29. Reaction steps “b” refer to the Suzuki reaction described in Example 29. Reaction step “c” is deacetylation, which was performed using a 33 wt. % methylamine in absolute ethanol solution (for compounds 3044 and 3049), as described in Example 29, or using the following Deacetylation Procedure B (compounds 3029, 2050, 2051).
Deacetylation Procedure B (Compounds 2050, 2051 and 3029)
NaOH (27 mg, 0.66 mmol) was added into a solution of compound 6 (110 mg, 0.066 mmol) in methanol-water (1:1, 5 mL) at room temperature, stirred (15 h) until the TLC indicated complete disappearance of the staring material. The reaction solution was acidified pH-2 with 3N aqueous HCl and the product was extracted with ethyl acetate (3×10 mL). The organic layers were combined and washed with brine, dried over Na2SO4, and concentrated in vacuo. The resulting residue was subjected for HPLC purification provided compound 3029 and followed the same procedure for compounds 2050, 2051.
aReagents and conditions: (a) DCM, 1N NaOH, TBAB, 2-bromophenol, r.t., 1 h; (b) Pd(PPh3)4, Cs2CO3,1,4-dioxane/water (5:1), 80° C., 1 h; (c) NaOH, methanol/water (1:1), r.t., overnight for 3029, 2050, and 2051 or 33% Methylamine in absolute ethanol, r.t., 1 h for 32-51; (d) CHCl3, 1N NaOH, TEBAC, 2-bromophenol, r.t., 1 h. See Examples 62-64 and Table 5 for identity of R1, R2, and R3.
The chemical identity of each new compound (3029, 3044 and 3049) and their spectral properties are described in Examples 63-65.
Compound was isolated as a white solid, 30 mg in 98% yield; NMR (400 MHz, CD3OD) δ ppm 8.75 (s, 1H), 8.54 (d, J=1.6 Hz, 1H), 8.41 (d, J=1.6 Hz, 1H), 7.47-7.35 (m, 4H), 7.22-7.14 (m, 1H), 5.08 (d, J=8.2 Hz, 1H), 4.03 (t, J=9.6 Hz, 1H), 3.91-3.74 (m, 3H), 3.73-3.60 (m, 2H), 1.58 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.67, 167.67, 156.07, 149.69, 141.81, 137.16, 133.74, 131.65, 131.54, 129.77, 124.17, 123.83, 117.13, 111.59, 101.67, 77.45, 73.24, 69.78, 62.63, 54.21, 22.69; LCMS (ESI): C21H22N2O10, found [M+Na]+, 485.2.
Compound was isolated as a white solid, 20 mg in 63% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.70-7.65 (m, 2H), 7.44-7.38 (m, 1H), 7.38-7.31 (m, 4H), 7.13-7.08 (m, 1H), 5.10 (d, J=8.2 Hz, 1H), 4.18 (dd, J=8.6, 10.6 Hz, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.85-3.73 (m, 2H), 3.72-3.61 (m, 2H), 1.72 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.09, 155.88, 140.47, 137.15, 131.80, 130.33, 129.85, 128.45, 124.18, 123.80, 121.26, 116.52, 100.88, 77.31, 73.38, 69.76, 62.59, 54.06, 22.94; LCMS (ESI): C22H23F3N2O7, found [M+Na]+, 507.4.
Compound was isolated as a white solid, 25 mg in 63% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.70-7.65 (m, 2H), 7.44-7.38 (m, 1H), 7.38-7.31 (m, 4H), 7.13-7.08 (m, 1H), 5.10 (d, J=8.2 Hz, 1H), 4.18 (dd, J=8.6, 10.6 Hz, 1H), 3.89 (d, J=3.1 Hz, 1H), 3.85-3.73 (m, 2H), 3.72-3.61 (m, 2H), 1.72 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 174.09, 155.88, 140.47, 137.15, 131.80, 130.33, 129.85, 128.45, 124.18, 123.80, 121.26, 116.52, 100.88, 77.31, 73.38, 69.76, 62.59, 54.06, 22.94; LCMS (ESI): C20H23F3N2O8S, found [M+Na]+, 543.2.
The ability of the newly synthesized Gal and GalNAc analogs 3029, 3044, and 3049 and previously synthesized compounds 29β-NAc, 2050, 2051, 2021, 2078, 2073, 2077, 2028, 2029, 2030, 2035, 2037, 2059, 2064, 2062, 2065, 2032, 1189, 2033, 2038, and 2043 to inhibit FmlH activity was assessed using a previously described enzyme-linked immunosorbent assay (ELISA) (Example 18). Briefly, 1 μg bovine submaxillary mucin (Sigma) in 100 uL PBS were incubated with Immulon 4HBX 96-well plates overnight prior to treatment with 1 mU Arthrobacter ureafaciens sialidase for 1 hour at 37° C. to remove terminal sialic acid sugars. Wells were then blocked with 200 μL PBS+1% BSA for 2 hours at room temperature. Biotinylated FmlHLD was diluted to 20 μg/mL in blocking buffer and incubated in the presence or absence of compounds serially diluted 2× down eight rows for 1 hour at room temperature. Wells were washed three times with PBS 0.05% TWEEN-20 then incubated with 100 μL of streptavidin-HRP conjugate (BD Biosciences; 1:2,000 dilution in blocking buffer) for one hour. After three additional PBS+0.05% TWEEN washes, plates were developed with 100 μL of tetramethylbenzidine (BD Biosciences) substrate and quenched with 50 μL of 1 M H2SO4. Total bound portion concentration was measured by the absorbance at 450 nm. IC50s were determined using the Graphpad Prism software.
This competitive binding assay measures the concentration of compound required to inhibit 50% of binding (IC50) to desialylated bovine submaxillary mucin, which contains high levels of Gal and GalNAc epitopes. The resultant IC50 values for each compound are shown in Table 5. The majority of compounds (2021, 2078, 2073, 2077, 2028, 2029, 2030, 2035, 2037, 2059, 2064, 2065, 2032, 1189, and 2033) had equal or slightly reduced potency relative parent compound 29β-NAc. It is noteworthy that the ortho-methoxy biphenyl GalNAc carboxylic analog 2051 showed the weakest activity with a 6-fold drop in activity (IC50, 3.87 μM) relative to 29β-NAc. This is most likely a result of forced ring twisting of the B-ring due to steric interference from the large ortho substituent. However, changing the carboxylic acid to a smaller phenol in compound 2073 increases the potency (IC50, 0.51 μM) back to the level of compound 29β-NAc and is equivalent to the desmethoxy analog 2021. The potency was slightly enhanced when the acid is replaced with a reverse amide as in 2062 (IC50, 0.31 μM), but decreases in the normal amide 1189 (IC50, 3.36 μM). However, the addition of a reverse methyl sulfonamide 2038 resulted in a 3-fold greater potency than 29β-NAc (IC50 0.23 μM), but as in amide 1189, the methyl sulfonamide derivative 2043 showed a loss in activity relative to 29β-NAc. This SAR suggests that distal placement of an H-bond acceptor (i.e., a carbonyl of the reverse amide or S═O bond of the sulfonamide) provides a greater binding benefit than a H-bond donor, presumably due to improved interactions with the Arg142 and/or Lys132 of FmlH. In general, it was discovered that groups which can accept an H-bond in the meta position of the B-ring show the best activity.
Similarly to the FmlH ligands shown in Example 23 (Table 3) the lead biphenyl GalNAc sulfonamide 2038 is more potent than its matched pair Gal derivative 2043 by about 5-fold. This trend has been demonstrated in all paired analogs hitherto synthesized. However, surprisingly, a reversal of this trend was seen when the potency of compounds 3029 and 2050 were assessed, as the B-ring disubstituted 3-nitro 5-carboxy analog 2050 (IC50, 0.28 μM) was 6-fold more active than the corresponding GalNAc version 3029 (IC50, 2.20 μM).
To determine the structural basis for the divergent SAR of Gal (2050) versus GalNAc (3029) and attempt to explain the unfavorable effect on binding from the N-acetyl group on GalNAc 3029 potency relative to Gal 2050, co-crystals were obtained and the X-ray structures of both 2050 and 3029 in complex with FimHLD were solved to 1.39 Å and 1.31 Å resolution, respectively (
FmlH protein used in crystallographic studies was expressed and purified as previously described (Kalas V et al., Structure-based discovery of glycomimetic FmlH ligands as inhibitors of bacterial adhesion during urinary tract infection. PNAS 2018). Briefly, protein was expressed in the periplasm of E. coli C600 cells containing pTRC99a encoding the first 182 amino acids of the CFT073 FmlH protein (corresponding to the signal sequence and lectin domain) and a C-terminal 6x-his tag. Periplasmic isolates prepared as previously described were washed over a cobalt affinity column (GoldBio) and eluted in 20 mM Tris 8.0+250 mM Imidazole. Fractions containing protein of the expected molecular weight were then diluted 5-fold in 20 mM Tris 8.0 to a final concentration of 50 mM Imidazole, washed over an anion exchange column (GE Healthcare Mono Q) with 20 mM Tris 8.0, and eluted in 20 mM Tris 8.0+250 mM NaCl. Resulting fractions were pooled and dialyzed in 1 mM HEPES pH 7.5+50 mM NaCl and concentrated as needed for further study. Note that FmlH protein purified in this way was also used in the ELISA assays described in Example 66 above. Protein used in ELISA assays was biotinylated using anNHS-PEG4-Biotin and Biotinylation Kits (ThermoFisher).
Crystallography Studies
All protein solutions were generated by adding 10 μL 50 mM compound dissolved in 100% DMSO to FmlH in 10 mM HEPES pH 7.5+50 mM NaCl immediately before setting up drops for a final concentration of 9 mg/mL FmlDLD, 5 mM compound, and 10% DMSO. Crystals of FmlH-3029 were grown by mixing 1 μL protein solution (9 mM FmlHLD, 5 mM compound 3029, 9 mM HEPES pH 7.5, 45 mM NaCl) with 1 μL 0.1 M Tris 8.0+0.8 M AmSO4 using the hanging drop vapor diffusion method. Square pyramidal crystals began appearing after approximately 24 hours and continued to grow for up to seven days. Crystals were harvested after 10 days, cryoprotected in a solution containing 0.1 M Tris 8.0, 0.8 M AmSO4, and 30% glycerol for 10 seconds, and flash-frozen in liquid nitrogen. To generate crystals of FmlH-2050, drops containing 9 mg/mL FmlH, 2.5 mM 2050, 10% DSMO, 0.1 M Tris 8.0 and 0.8 M AmSO4 were allowed to equilibrate over a 1M well solution of 0.1 M Tris 8.0+0.8 M AmSO4 for two days FmlH-2050 co-crystals were then transferred the pre-equilibrated drops and allowed to soak for 48 hours before cryoprotection in 0.1 M Tris 8.0, 0.8 M AmSO4, and 30% and flash-freezing in liquid nitrogen.
All data were collected on ALS Beamline 4.2.2 at an X-ray wavelength of 1.00 Å. Raw data were processed using XDS, Aimless, and Pointless (14, 15). The phase problem was solved using Phaser-MR in the Phenix suite using the apo FmlHLD structure (PDBID: 6AOW) as a search model (16). Iterative rounds of Phenix. Refine and Coot were used to refine the final model. Guided ligand replacement was performed using Phenix.
Surprisingly, it was found that the nitro group on the biphenyl B-ring, and not the carboxylic acid as previously observed, was bound in the pocket with R142. This contrasts with the FmlH co-crystal structure of 29β-NAc, in which the carboxylic acid occupies that pocket (
In an effort to further improve the potency of lead compound 2038, a series of additional rationally-directed modifications were explored. These include substitutions at the meta (R4) and para (R5)-positions of the biphenyl ring A while keeping the meta-substituted methyl sulfonamide B ring constant (3078-3085, 3090; Table 6). Also evaluated were different sulfonamides as in 3086-3087 and N-substitutions on the GalNAc ring including 3088 and 3089. This focused library of substituted biphenyl sulfonamide analogs (3086-3087) were synthesized as outlined in Scheme 2 and the N-substituted galactosamine derivatives 3088-3089 in Scheme 3. Compounds 3078-3085 and 3090 were synthesized following a similar reaction sequence as described in Scheme 1 (Examples 29 and 62). However, sulfonamide analogs 3086 and 3087 were prepared via sulfonylation of intermediate aniline 726. As shown in Scheme 3, GalNAc derivatives 3088 and 3089 were generated first by Koenig-Knorr type glycosylation reaction28 between 3,4,6-tri-O-acetyl-2-amino-2-deoxy-α-D-galactopynosyl bromide.HBr29 (52σ) and sodium 2-bromo-3-methylphenolate30 (53σ) to give bromide intermediate 62σ. Derivatization with trifluoroacetic acid anhydride or methanesulfonyl chloride yielded N-substituted galactosamine intermediates 75σ and 76σ. Subsequent Suzuki cross-coupling reaction with (3-(methylsulfonamido)phenyl)boronic acid followed by treatment with 33% Methylamine in absolute ethanol provided the target compounds 3088 and 3089.
Reaction schemes 2 and 3 are illustrated here and specific details are described in the following Example 68.
As described above in Example 68, new compounds 3078-3085 and 3090 were synthesized following a similar procedure to Example 29. Compounds 3086-3089 were each synthesized using newer protocols described in Schemes 2 and 3. However, for ease of reference, all synthesis procedures are described herein below (including those previously described in Example 29 which are represented in Scheme 1 and 2, steps (a), (b), and (e)). In general, the reactions performed were classified as “glycosylation”, Suzuki, or deacetylation reactions.
General Materials and Methods
Starting materials, reagents, and solvents were purchased from commercial vendors unless otherwise noted. In general, anhydrous solvents are used for carrying out all reactions. 1H and 13C NMR spectra were measured on a Varian 400 MHz and 100 MHz NMR spectrometers. The chemical shifts were reported as 8 ppm relative to TMS using residual solvent peak as the reference unless otherwise noted. The following abbreviations were used to express the peak multiplicities: s=singlet; d=doublet; t=triplet; q=quartet; m=multiplet; br=broad. High-performance liquid chromatography (HPLC) was carried out on GILSON GX-281 using Waters C18 5 μM, 4.6*50 mm and Waters Prep C18 5 μM, 19*150 mm reverse phase columns, eluted with a gradient system of 5:95 to 95:5 acetonitrile:water with a buffer consisting of 0.05-0.1% TFA. Mass spectroscopy (MS) was performed on HPLC/MSD using a gradient system of 5:95 to 95:5 acetonitrile:water with a buffer consisting of 0.05-0.1% TFA on a C18 or C8 reversed phased column and electrospray ionization (ESI) for detection. All reactions were monitored by thin layer chromatography (TLC) carried out on either Merck silica gel plates (0.25 mm thick, 60F254) and visualized by using UV (254 nm) or dyes such as 5% H2SO4 in ethanol. Silica gel chromatography was carried out on a Teledyne ISCO CombiFlash purification system using pre-packed silica gel columns (4 g to 80 g sizes). All compounds used for biological assays are greater than 95% purity based on NMR and HPLC by absorbance at 220 nm and 254 nm wavelengths.
Glycosylation Reactions
Method A (corresponding to step “a” in Scheme 2). Synthesis of (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(2-bromophenoxy)tetrahydro-2H-pyran-3,4-diyl diacetate (46). 1N aqueous NaOH solution (1 mL) was added into a solution of 2-Acetamido-3,4,6,-tri-O-acetyl-1-chloro-1,2-dideoxy-α-D-galactopyranose 226 (100 mg, 0.273 mmol), tetrabutylammonium bromide (88 mg, 0.273 mmol) and 2-bromo phenol (79 mg, 0.546 mmol) in dichloromethane (2 mL) at room temperature. Stir the reaction solution at the same temperature until the TLC indicates complete disappearance of chloride. Dilute the reaction mass with dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated undervacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate (2:3) combinations as eluent, giving rise to the compound 4σ and followed the same procedure for compounds 54σ-61σ (Note: Analytical data in SI)
Method B (step “a” in Scheme 1 only; not used in Schemes 2 or 3). (2R,3S,4S,5R,6S)-2-(acetoxymethyl)-6-(2-bromophenoxy)tetrahydro-2H-pyran-3,4,5-triyl triacetate (5σ). 1N aqueous NaOH solution (1 mL) was added into a solution of (2R,3S,4S,5R,6R)-2-(acetoxymethyl)-6-bromotetrahydro-2H-pyran-3,4,5-triyl triacetate 36 (200 mg, 0.487 mmol), benzyltriethylammonium chloride (111 mg, 0.0.487 mmol) and 2-bromo phenol (79 mg, 0.975 mmol) in chloroform (2 mL) at room temperature. Stir the reaction solution at 60° C. temperature until the TLC indicates complete disappearance of starting material. Cool the reaction solution and dilute with the dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate combinations as eluent, giving rise to the desired compound 5σ (Note: Analytical data in SI)
Method C corresponds to Scheme 3 and is divided into three steps (procedures 1-3) to result in the production of compounds 63σ and 64σ which are used to generate 3088 and 3089 using the Suzuki reaction and deacetylation procedures described below.
Procedure 1. Synthesis of (2R,3R,4R,5R,6S)-2-(acetoxymethyl)-5-amino-6-(2-bromo-3-methylphenoxy)tetrahydro-2H-pyran-3,4-diyl diacetate (62σ). Stir the solution of 3,4,6-Tri-O-acetyl-2-amino-2-deoxy-α-D-galactopyranosyl bromide.HBr, 52σ (550 mg, 1.363 mmol) and sodium 2-bromo-3-methyl phenol 53σ (570 mg, 2.726 mmol) in acetonitrile (40 mL) at room temperature for 24 h. Evaporate the solvent under reduced pressure, residue was diluted with DCM (50 mL) and washed with Sat.NaHCO3 and brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo and the resulting residue was purified by silica gel chromatography with hexane/ethyl acetate (2:3) provides the compound 62σ (Note: Analytical data in SI)
Procedure 2. Synthesis of (2R,3R,4R,5R,6S)-2-(acetoxymethyl)-6-(2-bromo-3-methylphenoxy)-5-(methylsulfonamido)tetrahydro-2H-pyran-3,4-diyl diacetate (63a). Trifluoroacetic anhydride (0.22 mL, 1.581 mmol) was added into a solution of (2R,3R,4R,5R,6S)-2-(acetoxymethyl)-5-amino-6-(2-bromo-3-methylphenoxy)tetrahydro-2H-pyran-3,4-diyl diacetate 62σ (250 mg, 0.527 mmol) and triethylamine (0.22 mL, 1.581 mmol) in dichloromethane (1 mL), stirred the solution at room temperature for 15 h. Dilute the reaction mass with dichloromethane (10 mL) and washed with Sat.NaHCO3 (5 mL) followed by brine (5 mL). The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate (2:3) combinations as eluent, giving rise to the compound 63σ (Note: Analytical data in SI).
Procedure 3. Synthesis of (2R,3R,4R,5R,6S)-2-(acetoxymethyl)-6-(2-bromo-3-methylphenoxy)-5-(methylsulfonamido)tetrahydro-2H-pyran-3,4-diyl diacetate (64σ). Methane sulfonyl chloride (119 mg, 1.038 mmol) was added into a solution of (2R,3R,4R,5R,6S)-2-(acetoxymethyl)-5-amino-6-(2-bromo-3-methylphenoxy)tetrahydro-2H-pyran-3,4-diyl diacetate 62σ (246 mg, 0.519 mmol) and triethylamine (0.22 mL, 1.556 mmol) in dichloromethane (1 mL), stirred the solution at room temperature for 1 h. Dilute the reaction mass with dichloromethane (10 mL) and washed with Sat.NaHCO3 (5 mL) followed by brine (5 mL). The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by silica gel chromatography with hexane/ethyl acetate (2:3) combinations as eluent, giving rise to the compound 646 (Note: Analytical data in SI).
Suzuki Reactions
The synthesis of compounds 3078-3085 and 3088-3090 used a Suzuki reaction procedure described in Example 29 (and here referred to as Procedure 1). The Suzuki reactions used in the synthesis of intermediates of 3086 (73σ) and 3087 (74σ) are described in Procedures 2 and 3, respectively.
Procedure 1. Under nitrogen atmosphere charge (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-(2-bromophenoxy) tetrahydro-2H-pyran-3,4-diyl diacetate (100 mg, 0.199 mmol), 3 (N-methyl amino carbonyl) phenyl boronic acid (78 mg, 0.298 mmol), Pd(PPh3)4 (23 mg, 0.0199 mmol) and Cesium carbonate (211 mg, 0.597 mmol) in reaction vial and add nitrogen gas bobbled 1,4-dioxane/water mixture (5:1, 3.6 mL) was added, heated the reaction solution to 80° C. Stir the reaction mixture at 80° C. until TLC indicates complete disappearance of staring material (1 h). Cool the reaction solution to RT and dilute with the dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated undervacuo. The resulting residue was purified by column chromatography with hexane/ethyl acetate (1:3) combinations as eluent, giving rise to the desired products 66-28σ (Scheme 1), 656-72σ (Scheme 2) and 756-77σ (Scheme 3)(Note: Analytical data in SI)
Procedure 2. Synthesis of (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-((3′-(cyclopropanesulfonamido)-6-methyl-[1,1′-biphenyl]-2-yl)oxy)tetrahydro-2H-pyran-3,4-diyl diacetate (73σ, acetylated form of 3086)). Cyclopropanesulfonyl chloride (54 mg, 0.189 mmol) was added into a solution of amine 72σ (100 mg, 0.378 mmol) and triethyl amine (0.08 mL, 0.567 mmol) in DCM (2.5 mL) at room temperature, stirred solution for 2 h. Dilute with the dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by column chromatography with hexane/ethyl acetate (3:2) giving rise to the desired products 736, acetylated form of 3086. (Note: Analytical data in SI).
Procedure 3. Synthesis of (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-((3′-((N,N-dimethylsulfamoyl)amino)-6-methyl-[1,1′-biphenyl]-2-yl)oxy)tetrahydro-2H-pyran-3,4-diyl diacetate (74σ, acetylated form of 3087). N,N-Dimethylsulfonyl chloride (54 mg, 0.189 mmol) was added into a solution of amine 72σ (100 mg, 0.378 mmol) and triethylamine (0.08 mL, 0.567 mmol) in DMF (2.5 mL) mixed in, stirred under microwaves at 80° C. for 2 h. Cool the reaction solution to RT and dilute with the dichloromethane (10 mL) and washed with water followed by brine. The organic layer was collected, dried over Na2SO4 and concentrated under vacuo. The resulting residue was purified by column chromatography with hexane/ethyl acetate (4:1) combinations as eluent, giving rise to the desired products 74σ (acetylated form of 3087). (Note: Analytical data in SI).
Deacetylation Reactions
Procedure 1. Synthesis of Compounds (3029). NaOH (27 mg, 0.66 mmol) was added into a solution of compound 6σ (110 mg, 0.066 mmol) in methanol-water (1:1, 5 mL) at room temperature, stirred (15 h) until the TLC indicated complete disappearance of the staring material. The reaction solution was acidified pH˜2 with 3N aqueous HCl and the product was extracted with ethyl acetate (3×10 mL). The organic layers were combined and washed with brine, dried over Na2SO4, and concentrated in vacuo. The resulting residue was subjected for HPLC purification provided compound 3029 and followed the same procedure for compounds 2050, 2051 and 3082.
Procedure 2. Excess amount of 33% Wt. methylamine in absolute ethanol solution (5 mL) was added into (2R,3R,4R,5R,6S)-5-acetamido-2-(acetoxymethyl)-6-((3′-hydroxy-[1,1′-biphenyl]-2-yl)oxy)tetrahydro-2H-pyran-3,4-diyl diacetate (50 mg, 0.097 mmol). Stir the reaction solution at the same temperature (0.5-1 h) until TLC indicates complete disappearance of staring material. Complete evaporation of the solvent provides the desired product compound 2021, which was subjected for HPLC purification and followed the same procedure for compounds 2078, 2073, 2077, 2028, 2029, 2030, 2035, 2037, 2059, 2064, 2062, 3044, 2065, 2032, 1189, 2033, 3049, 2038, and 2043 (described in Examples 30-60 and Example 62), 3078-3081 and 3083-3090.
Compound was isolated as a white solid, 30 mg in 94% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.63-7.58 (m, 1H), 7.57-7.49 (m, 3H), 7.39-7.33 (m, 1H), 7.23 (d, J=8.2 Hz, 1H), 7.16 (s, 1H), 6.98 (d, J=7.4 Hz, 1H), 5.11 (d, J=8.6 Hz, 1H), 3.97 (t, J=9.6 Hz, 1H), 3.87 (d, J=2.4 Hz, 1H), 3.85-3.73 (m, 2H), 3.73-3.67 (m, 1H), 3.63 (dd, J=2.4, 10.6 Hz, 1H), 3.00 (s, 3H), 1.78 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.81, 156.82, 152.49, 139.54, 135.32, 130.82, 130.48, 126.29, 121.27, 120.33, 118.20, 101.19, 77.60, 73.35, 69.74, 62.63, 53.92, 39.34, 23.16; LCMS (ESI): C21H25N3O10S, found [M+H]+, 512.3.
Compound was isolated as a white solid, 25 mg in 88% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.64 (dd, J=2.9, 6.46 Hz, 1H), 7.53-7.47 (m, 2H), 7.45-7.39 (m, 1H), 7.34-7.26 (m, 2H), 7.14 (d, J=7.4 Hz, 1H), 5.09 (d, J=8.6 Hz, 1H), 4.03-3.96 (m, 1H), 3.87 (d, J=2.7 Hz, 1H), 3.83-3.73 (m, 2H), 3.72-3.66 (m, 1H), 3.65-3.60 (m, 1H), 3.05 (s, 3H), 1.78 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.83, 156.43, 139.53, 136.87, 135.97, 131.11, 130.50, 128.08, 127.49, 121.62, 119.11, 114.82, 101.08, 77.57, 73.29, 69.74, 62.63, 53.95, 39.60, 23.11; LCMS (ESI): C22H25N3O8S, found [M+Na]+, 514.2.
Compound was isolated as a white solid, 7 mg in 59% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.40-7.28 (m, 2H), 7.25 (br. s., 2H), 7.14 (dd, J=8.0, 12.3 Hz, 2H), 6.88 (t, J=9.0 Hz, 1H), 5.10 (d, J=8.2 Hz, 1H), 4.00 (t, J=9.6 Hz, 1H), 3.87 (d, J=2.4 Hz, 1H), 3.84-3.73 (m, 2H), 3.71-3.60 (m, 2H), 3.03 (s, 3H), 1.77 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.91, 139.12, 133.95, 130.61, 130.01, 128.28, 124.36, 121.10, 112.25, 110.79, 110.56, 101.05, 77.42, 73.40, 69.74, 62.59, 54.09, 39.50, 23.11; LCMS (ESI): C21H25FN2O8S, found [M+H]+, 485.3.
Compound was isolated as a white solid, 37 mg in 90% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.52-7.45 (m, 1H), 7.45-7.40 (m, 2H), 7.30-7.23 (m, 3H), 7.18-7.10 (m, 2H), 7.06-7.00 (m, 2H), 6.94 (d, J=8.2 Hz, 1H), 5.06 (d, J=8.2 Hz, 1H), 5.00 (s, 2H), 4.01-3.93 (m, 1H), 3.85 (d, J=2.7 Hz, 1H), 3.84-3.71 (m, 2H), 3.70-3.64 (m, 1H), 3.60 (dd, J=3.1, 10.6 Hz, 1H), 2.90 (s, 3H), 1.76 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 170.06, 156.30, 139.07, 138.98, 134.93, 131.98, 130.13, 129.62, 129.30, 124.27, 123.41, 120.80, 119.40, 101.05, 77.43, 73.54, 69.75, 68.27, 62.62, 54.02, 39.46, 23.15; LCMS (ESI): C29H32N2O10S, found [M+H]+, 601.3.
Compound was isolated as a white solid, 22 mg in 92% yield; H NMR (400 MHz, CD3OD) δ ppm 7.49-7.37 (m, 3H), 7.33-7.27 (m, 1H), 7.19-7.13 (m, 2H), 7.00 (d, J=7.4 Hz, 1H), 5.06 (d, J=8.6 Hz, 1H), 4.00-3.91 (m, 1H), 3.86 (d, J=2.7 Hz, 1H), 3.83-3.72 (m, 2H), 3.71-3.65 (m, 1H), 3.61 (dd, J=3.1, 10.6 Hz, 1H), 2.99 (s, 3H), 1.78 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.87, 171.76, 156.34, 139.18, 138.89, 135.62, 132.08, 129.79, 124.11, 120.69, 119.15, 101.09, 77.41, 73.55, 69.77, 62.63, 54.07, 39.29, 23.18; LCMS (ESI): C22H26N2O10S, found [M+H]+, 511.2
Compound was isolated as a white solid, 13 mg in 93% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.28 (q, J=8.4 Hz, 2H), 7.18-7.14 (m, 2H), 7.04 (d, J=7.8 Hz, 1H), 6.95 (d, J=8.6 Hz, 1H), 6.77 (d, J=8.2 Hz, 1H), 5.06 (d, J=8.6 Hz, 1H), 3.93 (dd, J=8.6, 10.6 Hz, 1H), 3.86 (d, J=3.1 Hz, 1H), 3.82-3.73 (m, 2H), 3.71 (s, 3H), 3.68-3.59 (m, 2H), 3.02 (s, 3H), 1.78 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.90, 159.07, 156.88, 138.65, 136.71, 130.33, 129.54, 128.81, 124.94, 120.47, 109.18, 106.85, 100.95, 77.27, 73.56, 69.75, 62.58, 56.49, 54.21, 39.35, 23.16; LCMS (ESI): C22H28N2O9S, found [M+Na]+, 519.3.
Compound was isolated as a white solid, 31 mg in 97% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.35 (t, J=7.0 Hz, 1H), 7.23-7.16 (m, 2H), 7.15-7.10 (m, 1H), 7.07 (s, 1H), 6.95 (d, J=7.4 Hz, 2H), 5.01 (d, J=8.6 Hz, 1H), 3.94-3.71 (m, 4H), 3.68-3.56 (m, 2H), 3.01 (s, 3H), 2.05 (s, 3H), 1.81 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 171.92, 140.15, 138.68, 132.35, 130.18, 129.51, 125.15, 100.98, 77.21, 69.75, 62.58, 54.15, 39.46, 23.24, 20.79; LCMS (ESI): C22H28N2O8S, found [M+H]+, 481.4.
Compound was isolated as a white solid, 54 mg in 97% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.92-7.82 (m, 2H), 7.65-7.59 (m, 1H), 7.47-7.41 (m, 2H), 7.39-7.26 (m, 3H), 7.21 (d, J=12.9 Hz, 1H), 7.03 (d, J=7.0 Hz, 1H), 5.15 (d, J=8.6 Hz, 1H), 4.02-3.94 (m, 1H), 3.87 (br. s., 1H), 3.86-3.76 (m, 2H), 3.73-3.58 (m, 2H), 3.07 (s, 3H), 1.80 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.36, 129.10, 128.02, 127.62, 126.97, 126.39, 125.34, 124.61, 124.44, 120.75, 101.40, 39.59.23.25; LCMS (ESI): C25H28N2O8S, found [M+Na]+, 517.3
Compound was isolated as a white solid, 22 mg in 93% yield; 1H NMR (400 MHz, CD3OD) δ ppm 7.31-7.38 (m, 1H), 7.26-7.16 (m, 2H), 7.11 (d, J=9.8 Hz, 2H), 6.95 (d, J=7.4 Hz, 2H), 5.03 (d, J=8.2 Hz, 1H), 3.91-3.81 (m, 2H), 3.80-3.71 (m, 2H), 3.67-3.56 (m, 2H), 2.65-2.53 (m, 1H), 2.05 (s, 3H, 1.83 (s, 3H), 1.07-0.95 (m, 4H); 13C NMR (100 MHz, CD3OD) δ ppm 156.23, 139.99, 138.70, 132.40, 129.98, 125.10, 124.87, 100.85, 77.18, 69.73, 62.60, 54.22, 30.52, 23.24, 20.81, 6.06; LCMS (ESI): C24H30N2O8S, found [M+Na]+, 529.3.
Compound was isolated as a white solid, 21 mg in 89% yield; 1H NMR (400 MHz, DMSO-d6) δ ppm 9.77 (br. s., 1H), 7.56 (d, J=7.8 Hz, 1H), 7.28-7.17 (m, 2H), 7.14 (d, J=7.8 Hz, 1H), 7.02 (d, J=8.2 Hz, 1H), 6.82 (d, J=7.4 Hz, 1H), 4.93 (d, J=8.6 Hz, 1H), 3.79-3.69 (m, 1H), 3.66 (br. s., 1H), 3.57-3.47 (m, 3H), 3.47-3.40 (m, 1H), 2.70 (s, 6H), 1.99 (s, 3H), 1.74 (s, 3H); 13C NMR (100 MHz, DMSO-d6) δ ppm 171.99, 155.37, 135.52, 128.92, 128.12, 126.42, 125.47, 118.37, 99.26, 99.08, 75.89, 72.05, 71.74, 68.16, 61.03, 52.29, 37.60, 21.68; LCMS (ESI): C23H31N3O8S, found [M+Na]+, 532.3.
Compound was isolated as a white solid, 6 mg in 23% yield; 1H NMR (400 MHz, CD3OD) δ ppm 8.93 (d, J=9.4 Hz, 1H), 7.29 (t, J=6.5 Hz, 1H), 7.22 (br. s., 2H), 7.08-6.98 (m, 2H), 6.95 (d, J=7.4 Hz, 1H), 6.83 (br. s., 1H), 5.11 (d, J=8.6 Hz, 1H), 4.05-3.95 (m, 1H), 3.87 (d, J=2.4 Hz, 1H), 3.83-3.72 (m, 2H), 3.66 (d, J=9.4 Hz, 2H), 3.02 (br. s., 3H), 2.04 (s, 3H) (Note: 13C NMR not provide due to due to insufficient quantity); LCMS (ESI): C22H25F3N2O8S, found [M+Na]+, 557.3.
Compound was isolated as a white solid, 39 mg in 97% yield; 3H NMR (400 MHz, CD3OD) δ ppm 7.40-7.35 (m, 1H), 7.28-7.19 (m, 2H), 7.14 (d, J=9.8 Hz, 1H), 7.09-6.94 (m, 3H), 5.08 (d, J=16.8 Hz, 1H), 3.89 (br. s., 1H), 3.73 (d, J=5.9 Hz, 2H), 3.64-3.60 (m, 1H), 3.55-3.50 (m, 1H), 3.48-3.37 (m, 1H), 2.59-2.39 (m, 3H), 2.08 (br. s., 3H); 13C NMR (100 MHz, CD3OD) δ ppm 149.33, 130.37, 129.45, 125.22, 77.05, 69.81, 62.34, 57.85, 41.92, 39.63, 20.94; LCMS (ESI): C20H28N2O9S2, found [M+Na]+, 539.3
Compound was isolated as a white solid, 20 mg in 84% yield; 3H NMR (400 MHz, CD3OD) δ ppm 7.57-7.48 (m, 2H), 7.46-7.41 (m, 1H), 7.37-7.30 (m, 1H), 7.27-7.21 (m, 1H), 7.21-7.14 (m, 1H), 7.10 (s, 3H), 6.98-6.89 (m, 1H), 5.06 (d, J=8.0, 1H), 3.91-3.83 (m, 2H), 3.83-3.72 (m, 2H), 3.68 (t, J=6.1 Hz, 1H), 3.65-3.55 (m, 1H), 2.99 (s, 3H), 1.83 (s, 3H); 13C NMR (100 MHz, CD3OD) δ ppm 173.60, 157.18, 139.04, 137.11, 130.51, 129.72, 120.90, 120.39, 120.30, 119.97, 100.84, 77.48, 73.64, 73.34, 69.76, 62.63, 53.89, 39.20, 39.13, 23.28; LCMS (ESI): C22H25F3N2O8S, found [M+H]+, 535.3.
The potency of all compounds 3078-3090 were assessed using the ELISA assay described above to measure the IC50. These values are shown in Table 2. All N-acetyl compounds had excellent activity with an IC50 of 180 nM or better. We found that all analogs substituted with any of the various functional groups installed at the ortho position (R4) of the biphenyl A-ring (relative to the B-ring) further improved IC50s relative to lead compound 50 (R4=H). It is noteworthy that the cyclopropyl sulfonamide 3086 and the dimethyl sulfonyl urea derivative 3087 retain the same activity as the methyl sulfonamides. Compound 3090, containing the methyl sulfonamide in the meta position of the biphenyl B-ring and a trifluoromethyl group in the ortho R4 position on the B-ring exhibited the highest potency of the compounds tested, with an IC50 of 85 nM. Even the fused naphthyl A-ring 3085 has excellent potency with an IC50 of 0.18 μM. When the acetyl group of compound 3084 is replaced, the trifluoroacetamide retains potent activity (IC50 0.11 μM) while the methyl sulfonamide loses significant activity with an IC50 of only 3.5 μM. Table 6 summarizes the results.
To determine the molecular basis for the high potency exhibited by the biphenyl sulfonamides and the corresponding SAR, we solved an X-ray crystal structure of compound 3090 bound to FmlHLD. The co-crystal structure was solved to 1.75 Å resolution (
As previously described, all protein solutions were generated by adding 10 μL 50 mM compound dissolved in 100% DMSO to FmlH in 10 mM HEPES pH 7.5+50 mM NaCl immediately before setting up drops for a final concentration of 9 mg/mL FmlDLD, 5 mM compound, and 10% DMSO. Co-crystals of FmlH-3090 (AM2134) were grown by mixing 14 protein solution with 14 mother liquor containing 0.7 M LiSO4+20% PEG 8000 on a glass coverslip over 1 mL mother liquor. Thin, needle-like crystals appeared after approximately 72 hours. Crystals were cryoprotected in 1 M LiSO4+20% PEG 8000+25% glycerol for 10 seconds before and flash-freezing in liquid nitrogen. All data were collected on ALS Beamline 4.2.2 at an X-ray wavelength of 1.00 Å. Raw data were processed using XDS, Aimless, and Pointless (14, 15). The phase problem was solved using Phaser-MR in the Phenix suite using the apo FmlHLD structure (PDBID: 6AOW) as a search model (16). Iterative rounds of Phenix. Refine and Coot were used to refine the final model. Guided ligand replacement was performed using Phenix.
As previously observed in the 29β-NAc-FmlHLD co-crystal structure (
Due to the labile nature of the 0-glycosidic linkage of the biphenyl Gal and GalNAc FmlH antagonists, studies were pursued to evaluate their stability. To evaluate their therapeutic potential for advancing into planned animal studies, the in vitro stability was assessed of six leading compounds 3079, 3080, 3084, 3086, 3088, and 3090 based on their potency and structural diversity (Table 6). These compounds were assessed for their stability in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), mouse liver microsomes, and blood plasma (Table 7). All compounds tested exhibited a high degree of stability, with some variation seen in the plasma stability. These findings are consistent with our earlier characterization of FimH antagonists (mannosides). In these studies, it was demonstrated that the lability of the 0-glycosidic linkage both in vitro and in vivo resulted in the appearance and detection of the phenol product of metabolism in mouse plasma and urine. The two most stable analogs, 3086 and 3090, were subjected to further testing for their pharmacokinetics (PK) in rats.
In this example, the concentration of compounds 3086 and 3090 in rat plasma and urine following either a 10 mg/kg oral dose (PO; circular dots) or a 3 mg/kg intravenous dose (IV; square dots) was determined (
In order to determine if the improved PK properties of 3086 relative to 3090 are a consequence of the CH3 versus CF3 group on the biphenyl A-ring or the cyclopropyl sulfonamide versus methyl sulfonamide of the B-ring, an additional study was performed in mice with compound 3084, the methyl sulfonamide derivative of 3086 or the CH3 derivative of 3090. This enabled the determination of the isolated effects of a single substitution. These studies were conducted via 20 mg/kg intraperitoneal (IP) injection to inform planned future IP studies in murine studies of chronic UTI, which require a single IP dose of galactoside to persist in the plasma for 6 hours prior to measurement of bladder bacterial burdens (
While not a perfect comparison to 3086 and 3090, the half-life, t1/2 in the mouse is calculated to be 1.13 h and the clearance rate appears to be slower than either that of 3086 or 3090. The compound 3084 shows moderate compound exposure at 8 h with a Cmax of 7897 ng/mL and a calculated AUC of 6300 ng·h/mL. This compound has an IC50 of 120 nM, which equates to a concentration of 57.7 ng/mL. At the 4 h timepoint the average concentration of 3084 was 79.5 ng/mL. By extrapolating these kinetics we can infer that the plasma concentration of this compound would likely remain well above the IC50 for the 6 h, the exact timeframe required for our murine model of chronic UTI.
The compounds synthesized herein were tested in the ELISA-based competition assay for their ability to inhibit binding of FmlHLD to ds-BSM. The ELISA assay was performed using the protocol described in Example 18. Compounds were tested at 10004, 1004, 1 μM, and 100 nM. as indicated on the table. In addition, the Kd and IC50 values for some of the compounds are noted. Results are summarized in Table 8. ND means the corresponding value was not determined for this summary (but may be available elsewhere in the Examples).
The following abbreviations used herein are defined as follows:
Ac=acetyl; Ac2O=aceic anhydride; Bn=benzyl; BnBr=benzyl bromide; OSO4=osmium tetraoxide; BCl3=boron trichloride; NaIO4=sodium periodate; CuSO4=copper sulfate; n-BuiLi=n-butyl lithium; Cy=cyclohexyl; dba=dibenzylideneacetone; DCI=4,5-dicyanoimidazole; DDTT=3-((dimethylaminomethylidene)amino)-3H-1,2,4-dithiazole-5-thione; DMA=N,N-dimethylacetamide; DMAP=4-Dimethylaminopyridine; DMOCP=2-chloro-5,5-dimethyl-2-oxo-1,3,2-dioxaphosphorinane; DMP=Dess-Martin periodinane; DMTr=dimethoxytrityl=(4-methoxyphenyl)2 (phenyl)methyl; Piv=pivaloyl=(CH3)3C—C(═O)—; NaOH=sodium hydroxide; NaH=sodium hydride; M=molar; nM=nanomolar; μM=micromolar mL=milliliter; h=hour; min.=minute; HCl=hydrogen chloride; H2O=water; MS=mass spectrometry; LCMS=Liquid chromatography/mass spectrometry; ES+=electrospray positive ionization; 1H-NMR=proton nuclear magnetic resonance; 13C-NMR=carbon-13 nuclear magnetic resonance; 31P-NMR=phosphorous-31 nuclear magnetic resonance; MHz=megahertz; H=hydrogen; RT=rt=room temperature; ° C.=Celsius; Br2=bromine; NaHSO3=sodium bisulfite; NMP=N-Methyl-2-pyrrolidone; NMM=N-methyl morpholine; NMO=N-methyl morpholine N-oxide; MW=microwave; KF=potassium fluoride; Pd (dppf)Cl2=[1,1′-bis (diphenylphosphino)ferrocene]palladium (II) dichloride; PE=petroleum ether; EtOAc=EA=EtOAc; CDCl3=deuterated chloroform; DMSO-d6=dimethyl sulfoxide deuterated-6; CD3CN=deuterated acetonitrile; LTBA=lithium tri (tert-butoxy)aluminium hydride=LiAlH (Ot-Bu)3; MeOH=methanol; NaOMe=sodium methoxide; D2O=deuterated water; prep-HPLC=preparative high pressure liquid chromatography, also known as preparative high performance liquid chromatography; DMSO=dimethyl sulfoxide; MeCN=CH3CN=acetonitrile; CH3I=methyl iodide; NH3=ammonia; NH4OH=ammonium hydroxide; NIS=N-iodosuccinimide; DMF=N, N-dimethylformamide; K3PO4=potassium phosphate, tribasic; N2=nitrogen; Py=pyridine; THF=tetrahydrofuran; Cs2CO3=cesium carbonate; Na2CO3=sodium carbonate; NaHCO3=sodium bicarbonate; Na2SO4=sodium sulfate; TEA=triethylamine; TBSCl=tert-butyldimethylsilyl chloride; TMSCl=trimethylsilyl chloride; TMS=trimethylsilyl; TMSOTf=trimethylsilyl triflate; TFA=trifluoroacetic acid; DCM=CH2Cl2=dichloromethane; Hunig's base=DIPEA=iPr2NEt=N, N-diisopropylethylamine; K2CO3=potassium carbonate; KOAc=potassium acetate; μl=microliter; g=gram; mg=milligram.
When introducing elements of the present invention or the preferred embodiments(s) thereof, the articles “a”, “an”, “the” and “said” are intended to mean that there are one or more of the elements. The terms “comprising”, “including” and “having” are intended to be inclusive and mean that there may be additional elements other than the listed elements.
In view of the above, it will be seen that the several objects of the invention are achieved and other advantageous results attained.
As various changes could be made in the above compounds, compositions and methods without departing from the scope of the invention, it is intended that all matter contained in the above description and shown in the accompanying figures shall be interpreted as illustrative and not in a limiting sense.
Having described the invention in detail, it will be apparent that modifications and variations are possible without departing from the scope of the invention defined in the appended claims.
This application claims priority of U.S. provisional application Ser. No. 62/583,357 filed on Nov. 8, 2017, and U.S. provisional application Ser. No. 62/726,303 filed on Sep. 2, 2018, the entire disclosures of which are incorporated herein by reference.
This invention was made with government support under DK108840 awarded by the National Institutes of Health. The government has certain rights in the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2018/059877 | 11/8/2018 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62726303 | Sep 2018 | US | |
| 62583357 | Nov 2017 | US |
