Claims
- 1. A compound having a double chain and which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein at least one portion of said DNA (-chain) has been replaced by a 2'-O-substituted RNA, wherein said 2'-O-substituted RNA has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA, and wherein, when said compound is subjected to the action of an enzyme having ribonuclease H activity, the RNA (+chain) is cleaved at a position corresponding to the unreplaced portion of the DNA (-chain).
- 2. The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a methyl group.
- 3. The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on all hydroxyl groups of the ribose moieties of the RNA by a methyl group.
- 4. The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by an ethyl group.
- 5. The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a propyl group.
- 6. A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein said 2'-O-substituted RNA oligomer has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA oligomer, wherein said 2'-O-substituted RNA oligomer is conjugated to said DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety.
- 7. The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a methyl group.
- 8. The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on all 2'-hydroxyl groups of the ribose moieties of the RNA by a methyl group.
- 9. The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by an ethyl group.
- 10. The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a propyl group.
- 11. A nucleoside compound of the formula: ##STR37## wherein X is a monomethoxy-trityl or dimethoxy-trityl radical, Y represents --P(OCH.sub.3)--N--(CH(CH.sub.3).sub.2).sub.2, --P(OCH.sub.2 CH.sub.2 CN)--N--(CH(CH.sub.3).sub.2).sub.2, or --CO(CH.sub.2).sub.m --CO--NH--(CH.sub.2).sub.n-(CPG), m and n are each an integer of 1 to 10, and R represents a radical of one of the formulae: ##STR38##
- 12. The nucleoside compound of claim 11, wherein R is: ##STR39##
- 13. The nucleoside compound of claim 11, wherein R is: ##STR40##
- 14. The nucleoside compound of claim 11, wherein R is: ##STR41##
- 15. The nucleoside compound of claim 11, wherein R is: ##STR42##
- 16. A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein the 2'-O-substituted RNA is conjugated to the DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety, wherein the DNA oligomer is from 3 to 6 bases long.
- 17. The mixed oligomer of claim 16, wherein at least one, and up to all, of the 2'-hydroxyl groups in the sugar portion of the RNA has a substituent.
Priority Claims (2)
Number |
Date |
Country |
Kind |
61-211157 |
Sep 1986 |
JPX |
|
62-13726 |
Jan 1987 |
JPX |
|
Parent Case Info
This application is a continuation of application Ser. No. 07/092,198, filed on Sept. 2, 1987, now abandoned.
US Referenced Citations (5)
Foreign Referenced Citations (3)
Number |
Date |
Country |
0090789 |
Mar 1982 |
EPX |
56-44400 |
Feb 1982 |
JPX |
0004199 |
Jan 1985 |
JPX |
Non-Patent Literature Citations (2)
Entry |
Hideo Inoue, et al, Nucleic Acids Research, Symposium Series No. 16, 1985. |
Koster, H. et al. N--acyl Protecting Groups for Deoxynucleosides. A Quantitative and Comparative Study (1981). |
Continuations (1)
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Number |
Date |
Country |
Parent |
92198 |
Sep 1987 |
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