Patent Application
20130035321
Retinoblastoma (Rb) is an ocular cancer that affects approximately 5,000 to 8,000 children worldwide each year and constitutes the most common primary ocular tumor of childhood (Abramson, Invest. Ophthalmol. Vis. Sci. 2005, 46, 2683-2691). This type of cancer occurs in germline (40%) and non-germline (60%) forms and results from the loss of function of both alleles of the retinoblastoma tumor suppressor gene (Rb1) in retinal progenitor cells (Cavenee et al., Science 1985, 228, 501-503; Friend et al., Nature 1986, 323, 643-646; Godbout et al., Nature 1983, 304, 451-453). Given that the primary role of the retinoblastoma pathway is to regulate cell proliferation, loss of Rb1 plays an important role in cancer development in both ocular and nonocular cancers.
The current survival rate associated with retinoblastoma is approximately 90% in developing countries (De Potter et al., Curr. Opin. Ophthalmol. 2002, 13, 331-336). However, effective treatment is often drastic and may require removal of the affected eye (i.e., enucleation). Although other forms of treatment exist, they are usually used as supplements to enucleation in multimodal treatment regimens rather than substitutes thereof. Exemplary combinations of such multimodal treatment regimens include the use of external beam therapy and/or chemotherapy to shrink the tumor prior to local treatment such as thermotherapy, cryotherapy, and brachytherapy (Wilson, et al., Investigative Ophthalmology & Visual Science, 2006, 47(4), 1269-1273). Unfortunately, complications from the use of multimodal treatment approaches can be severe and are particularly harmful to young children.
Complications from radiotherapy and/or systemic chemotherapy are especially common in children genetically predisposed to tumor development as a result of, for instance, loss of Rb1. Long term effects of external beam radiotherapy can include cataracts, radiation retinopathy, impaired vision, temporal bone suppression (De Potter et al., Curr. Opin. Ophthalmol. 2002, 13, 331-336), and an increased incidence of secondary cancers, particularly for children under the age of one. Side effects of systemic chemotherapy are similarly undesirable and may include cytopenia, neutropenia, gastrointestinal distress, and neurotoxicity (Brichard et al., Med. Pediatr. Oncol. 2002, 38, 411-415; Benz et al., Arch. Ophthalmol. 2000, 118, 577-578; Beck et al., J. Clin. Oncol. 2000, 18, 2881-2887; Friedman et al., J. Clin. Oncol. 2000, 18, 12-17; Rizzuti et al., Arch. Ophthalmol. 2008, 126, 862-865). Platinum-based drugs used for the treatment of childhood malignancies have been shown to increases a child's risk of developing secondary malignancies, and etoposide, a topoisomerase II inhibitor also used to treat certain types of childhood cancers, has been linked to the development of secondary leukemias (Klein et al., Eur. J. Cancer 2003, 39, 808-817; Nishimura et al., J. Clin. Oncol. 2001, 19, 4182-4183; Chantada et al., Med. Pediatr. Oncol. 2003, 40, 158-161). Consequently, available treatments for retinoblastoma are limited and often devastating, necessitating the development of new, more effective alternatives.
Retinoblastoma treatment regimens incorporating chemotherapy stand to benefit substantially from drug delivery of the chemotherapeutic agent into the ophthalmic artery. One such treatment method currently being pursued is direct intraarterial infusion (Abramson et al., Ophthalmology 2008, 115(8), 1398-1404). Intraarterial infusion delivers the drug locally and provides a promising new approach to chemotherapy by preventing toxic agents from entering the systemic circulation. Minimized systemic exposure affords many drugs diminished toxicity and improved efficacy to the extent that reinvestigation of chemotherapeutic agents previously thought to be unsuitable (e.g., cardenolides) may now lead to new treatment options when combined with intraarterial infusion.
The present invention describes a chemical screen aimed specifically at identifying alternative chemotherapeutic agents for local delivery in the treatment of retinoblastoma. Many of the inventive compounds screened herein are novel derivatives of a class of compounds known as the cardenolides. Cardenolides were first identified as potentially useful chemotherapeutic agents in an earlier screen that assayed the activity of libraries of known compounds against various cancer cell lines. Currently, cardenolides are used in the clinic for the treatment of heart disease. Many members of this class of compounds have well-characterized and well-defined pharmacological profiles. Thus, cardenolides provide an attractive scaffold for synthesizing novel compounds and derivatives for use in the treatment of retinoblastoma, particularly via local administration of the inventive compound into the ophthalmic artery.
In one aspect, the present invention provides novel compounds that may be used to treat an ocular cancer (e.g., retinoblastoma). In another aspect, the present invention provides a method of treating an ocular cancer comprising administering a therapeutically effective amount of a compound of the instant invention locally to the eye of a subject with an ocular cancer. In yet another aspect, the invention provides pharmaceutical compositions of the inventive compounds. In some embodiments, the inventive compound useful in treating an ocular cancer is of the formula:
wherein:
the dashed line between C4 and C5 indicates that the bond between C4 and C5 is either a single or a double bond; and wherein the dashed line between C4 and C5 cannot be a double bond if the bond between C5 and C6 is a double bond.
the dashed line between C5 and C6 indicates that the bond between C5 and C6 is either a single or a double bond; and wherein the dashed line between C5 and C6 cannot be a double bond if the bond between C4 and C5 is a double bond.
the dashed line between C16 and C17 indicates that the bond between C16 and C17 is either a single or a double bond;
R1 is
R2 is H, OH, OAc, or ORb, wherein Rb is a suitable protecting group;
R3 is H, OH, OAc, or ORc, wherein Rc is a suitable protecting group;
R4 is H, OH, OAc, or ORd, wherein Rd is a suitable protecting group;
R5 is H, C1-6 alkyl, OH, OAc, or ORe, wherein Re is a suitable protecting group; wherein R4 and R5 may be optionally taken together to form an epoxide;
R6 is H, OH, OAc, or ORf, wherein Rf is a suitable protecting group; and wherein L is an integer from 0 to 1;
R7 is H, C1-6 alkyl, OH, OAc, or ORg, wherein Rg is a carbohydrate moiety or a suitable protecting group;
R8 is H, OH, OAc, C1-6 alkyl, CHO, CH2OH, CH2ORh, CO2H, CO2Rh, or ORh, wherein Rh is a suitable protecting group; and
R9 is H, OH, OAc, or ORi, wherein Ri is a suitable protecting group;
or a pharmaceutically acceptable salt thereof.
In certain embodiments, R7 is ORg. In some embodiments, Rg is a carbohydrate moiety. In certain embodiments, Rg is a glycoside. In certain embodiments, Rg is a starch, glycogen, dextran, cyclodextran, or hyaluranic acid. In some embodiments, Rg is an oligosaccharide. In certain embodiments, Rg is a disaccharide such as sucrose, lactose, or maltose. In some embodiments, Rg is a monosaccharide such as glucose, fructose, galactose, mannose, xylose, or ribose. In some embodiments, Rg is a carbohydrate derivative (e.g., an ester, ether, aminated, amidated, sulfated, phosphosubstituted, or otherwise suitably protected carbohydrate. (e.g., an ester, ether, aminated, amidated, sulfated, phosphosubstituted, or otherwise suitably protected carbohydrate).
In some embodiments, the compound of the invention is of the formula:
wherein:
R1 is
R2 is H, OH, OAc, or ORb′, wherein Rb is a suitable protecting group;
R3 is H, OH, OAc, or ORc′, wherein Rc is a suitable protecting group;
R4 is H, C1-6 alkyl, OH, OAc, or ORd′, wherein Rd is a suitable protecting group;
R5 is H, OH, OAc, or ORe, wherein Re is a suitable protecting group;
R6 is H, OH, OAc, or ORf, wherein Rf is a suitable protecting group;
R7 is H, C1-6 alkyl, CHO, CH2OH, CH2ORg, CO2H, or CO2Rg wherein Rg is a suitable protecting group; and
R8 is H or C1-6 alkyl; or a pharmaceutically acceptable salt thereof.
In certain embodiments, the compound of the invention is of any of the formulae:
In some embodiments, the invention provides a method of treating a subject with an ocular cancer comprising administering a therapeutically effective amount of an inventive compound locally to the eye with the cancer. In certain embodiments, the invention also provides pharmaceutical compositions containing a therapeutically effective amount of an inventive compound or a pharmaceutically acceptable salt thereof. In some embodiments, the pharmaceutical composition is suitable for intraarterial infusion.
Exemplary ocular cancers that may be treated using the inventive compounds or methods include, but are not limited to, retinoblastoma (Rb), medulloepithelioma, ocular melanoma, lymphoma, or other cancers which have metastasized to the eye. In some embodiments, the ocular cancer is retinoblastoma. In some embodiments, the treatment method further comprises a second type of therapy. In certain embodiments, the second type of therapy comprises administration of a second chemotherapeutic agent. In certain embodiments, the second type of therapy is radiation therapy. In another aspect, the instant invention provides a method of treating an ocular cancer comprising administering a therapeutically effective amount of a compound of the invention via direct intraarterial infusion into the ophthalmic artery of the eye of a subject with ocular cancer. In certain embodiments, the subject is a human. Exemplary ocular cancers that may be treated by local administration of compound of the invention include, but are not limited to, ocular cancers such as retinoblastoma, medulloepithelioma, ocular melanoma, lymphoma, or other cancer which has metastasized to the eye. In certain embodiments, the cancer being treated based on the present invention is retinoblastoma.
In another aspect, the instant invention provides a method of inhibiting the growth of ocular cancer cells comprising contacting in vitro ocular cancer cells with an effective amount of a compound of the invention to inhibit the growth of the cells. In some embodiments, inhibiting the growth of ocular cancer cells further comprises contacting the cells with a second chemotherapeutic agent in combination with the compound of the invention. In certain embodiments, the cells are derived from a retinoblastoma tumor. Exemplary human retinoblastoma cell lines include Y79, WERI-Rb-1, RB355, or Y79LUC.
In certain embodiments, the instant invention provides a pharmaceutical composition for treating an ocular cancer via local administration comprising a compound of the invention and a pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical composition comprising a compound of the invention is administered via direct intraarterial infusion into the ophthalmic artery of the eye of a subject with one of the above-mentioned cancers.
The instant invention also provides methods for treating cancer by locally administering a compound of the instant invention and a pharmaceutically acceptable excipient to a subject in need thereof. In certain embodiments, local administration comprises intraarterial infusion, and the cancer is a hematopoietic cancer, liposarcoma, lung cancer, brain cancer, liver cancer, or pancreatic cancer. In some embodiments, the subject with one of the above-mentioned cancers is a mammal, e.g., a human, mouse, rat, guinea pig, dog, cat, horse, cow, pig, or non-human primate, such as a monkey, chimpanzee, or baboon. In one embodiment, the subject is a human. In some embodiments, the subject is a bird, reptile, amphibian, fish, and/or worm. In some embodiments, an animal may be an experimental animal, a transgenic animal, genetically-engineered animal, and/or a clone. In certain embodiments, the subject is a human under the age of 18. In certain embodiments, the subject is a human under the age of 12. In some embodiments, the subject is a human under the age of 5, 4, 3, 2, or 1.
All publications and patent documents cited in this application are incorporated by reference in their entirety for all purposes to the same extent as if the contents of each individual publication or patent document were incorporated herein.
Definitions of specific functional groups and chemical terms are described in more detail below. For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75th Ed., inside cover, and specific functional groups are generally defined as described therein. Additionally, general principles of organic chemistry, as well as specific functional moieties and reactivity, are described in Organic Chemistry, Thomas Sorrell, University Science Books, Sausalito, 1999; Smith and March, March's Advanced Organic Chemistry, 5th Edition, John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; Carruthers, Some Modern Methods of Organic Synthesis, 3rd Edition, Cambridge University Press, Cambridge, 1987; the entire contents of each of which are incorporated herein by reference.
It will be appreciated that the compounds, as described herein, may be substituted with any number of substituents or functional moieties. In general, the term “substituted” whether preceded by the term “optionally” or not, and substituents contained in formulas of this invention, refer to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent. When more than one position in any given structure is substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. As used herein, the term “substituted” is contemplated to include all permissible substituents of organic compounds. In a broad aspect, the permissible substituents include acyclic and cyclic, branched and unbranched, carbocyclic and heterocyclic, aromatic and nonaromatic, aliphatic and heteroaliphatic, carbon and heteroatom substituents of organic compounds. For purposes of this invention, heteroatoms such as nitrogen may have hydrogen substituents and/or any permissible substituents of organic compounds described herein which satisfy the valencies of the heteroatoms. Furthermore, this invention is not intended to be limited in any manner by the permissible substituents of organic compounds. Combinations of substituents and variables envisioned by this invention are preferably those that result in the formation of stable compounds useful in the treatment, for example of proliferative diseases, including, but not limited to cancer. The term “stable”, as used herein, typically refers to compounds which possess stability sufficient to allow manufacture and which maintain the integrity of the compound for a sufficient period of time to be detected and preferably for a sufficient period of time to be useful for the purposes detailed herein.
Certain compounds of the present invention can comprise one or more asymmetric centers, and thus can exist in various isomeric forms, e.g., stereoisomers and/or diastereomers. Thus, inventive compounds and pharmaceutical compositions thereof may be in the form of an individual enantiomer, diastereomer, or geometric isomer, or may be in the form of a mixture of stereoisomers. In certain embodiments, the compounds of the invention are enantiopure compounds. In certain other embodiments, mixtures of stereoisomers or diastereomers are provided.
Furthermore, certain compounds, as described herein may have one or more double bonds that can exist as either the Z or E isomer, unless otherwise indicated. The invention additionally encompasses the compounds as individual isomers substantially free of other isomers and alternatively, as mixtures of various isomers, e.g., racemic mixtures of stereoisomers. In addition to the above-mentioned compounds per se, the invention also encompasses pharmaceutically acceptable derivatives of these compounds and compositions comprising one or more compounds.
Where a particular enantiomer is desired, it may, in some embodiments be provided substantially free of the corresponding enantiomer, and may also be referred to as “optically enriched.” “Optically enriched,” as used herein, means that the compound is made up of a significantly greater proportion of one enantiomer. In certain embodiments the compound is made up of at least about 90% by weight of a particular enantiomer. In other embodiments the compound is made up of at least about 95%, 96%, 97%, 98%, or 99% by weight of a desired enantiomer. A desired enantiomer may be isolated from a racemic mixture by any method known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) or the formation and crystallization of chiral salts, or the enantiomer may be prepared by asymmetric syntheses. See, for example, Jacques et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen et al., Tetrahedron 33:2725 (1977); Eliel, Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); Wilen, Tables of Resolving Agents and Optical Resolutions p. 268 (E. L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, Ind. 1972).
The term “acyl”, as used herein, refers to a carbonyl-containing functionality, e.g., —C(═O)R′, wherein R′ is an aliphatic, alicyclic, heteroaliphatic, heterocyclic, aryl, heteroaryl, (aliphatic)aryl, (heteroaliphatic)aryl, heteroaliphatic(aryl), or heteroaliphatic(heteroaryl) moiety, whereby each of the aliphatic, heteroaliphatic, aryl, or heteroaryl moieties is substituted or unsubstituted, or is a substituted (e.g., hydrogen; or aliphatic, heteroaliphatic, aryl, or heteroaryl moieties) oxygen or nitrogen containing functionality (e.g., forming a carboxylic acid, ester, or amide functionality).
The term “aliphatic”, as used herein, includes both saturated and unsaturated, straight chain (i.e., unbranched) or branched aliphatic hydrocarbons, which are optionally substituted with one or more functional groups. As will be appreciated by one of ordinary skill in the art, “aliphatic” is intended herein to include, but is not limited to, alkyl, alkenyl, and alkynyl moieties. Thus, as used herein, the term “alkyl” includes straight and branched alkyl groups. An analogous convention applies to other generic terms such as “alkenyl”, “alkynyl” and the like. Furthermore, as used herein, the terms “alkyl”, “alkenyl”, “alkynyl” and the like encompass both substituted and unsubstituted groups. In certain embodiments, as used herein, “lower alkyl” is used to indicate those alkyl groups (substituted or unsubstituted, branched or unbranched) having 1-6 carbon atoms.
In certain embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-20 aliphatic carbon atoms. In certain other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-10 aliphatic carbon atoms. In yet other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-8 aliphatic carbon atoms. In still other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-6 aliphatic carbon atoms. In yet other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-4 carbon atoms. Illustrative aliphatic groups thus include, but are not limited to, for example, methyl, ethyl, n-propyl, isopropyl, allyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, sec-pentyl, isopentyl, tert-pentyl, n-hexyl, sec-hexyl, and the like, which again, may bear one or more substituents. Alkenyl groups include, but are not limited to, for example, ethenyl, propenyl, butenyl, 1-methyl-2-buten-1-yl, and the like. Representative alkynyl groups include, but are not limited to, ethynyl, 2-propynyl(propargyl), 1-propynyl, and the like.
The term “alicyclic,” as used herein, refers to compounds which combine the properties of aliphatic and cyclic compounds and include but are not limited to cyclic, or polycyclic aliphatic hydrocarbons and bridged cycloalkyl compounds, which are optionally substituted with one or more functional groups. As will be appreciated by one of ordinary skill in the art, “alicyclic” is intended herein to include, but is not limited to, cycloalkyl, cycloalkenyl, and cycloalkynyl moieties, which are optionally substituted with one or more functional groups. Illustrative alicyclic groups thus include, but are not limited to, for example, cyclopropyl, —CH2-cyclopropyl, cyclobutyl, —CH2-cyclobutyl, cyclopentyl, —CH2-cyclopentyl, cyclohexyl, —CH2-cyclohexyl, cyclohexenylethyl, cyclohexanylethyl, norborbyl moieties and the like, which again, may bear one or more substituents.
The term “alkoxy” (or “alkyloxy”), or “thioalkyl” as used herein refers to an alkyl group, as previously defined, attached to the parent molecular moiety through an oxygen atom or through a sulfur atom. In certain embodiments, the alkyl group contains 1-20 aliphatic carbon atoms. In certain other embodiments, the alkyl group contains 1-10 aliphatic carbon atoms. In yet other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-8 aliphatic carbon atoms. In still other embodiments, the alkyl group contains 1-6 aliphatic carbon atoms. In yet other embodiments, the alkyl group contains 1-4 aliphatic carbon atoms. Examples of alkoxy, include but are not limited to, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, tert-butoxy, neopentoxy, and n-hexoxy. Examples of thioalkyl include, but are not limited to, methylthio, ethylthio, propylthio, isopropylthio, n-butylthio, and the like.
The term “alkylamino” refers to a group having the structure —NHR′ wherein R′ is alkyl, as defined herein. The term “aminoalkyl” refers to a group having the structure —NH2R′, wherein R′ is alkyl, as defined herein. In certain embodiments, the alkyl group contains 1-20 aliphatic carbon atoms. In certain other embodiments, the alkyl group contains 1-10 aliphatic carbon atoms. In yet other embodiments, the alkyl, alkenyl, and alkynyl groups employed in the invention contain 1-8 aliphatic carbon atoms. In still other embodiments, the alkyl group contains 1-6 aliphatic carbon atoms. In yet other embodiments, the alkyl group contains 1-4 aliphatic carbon atoms. Examples of alkylamino include, but are not limited to, methylamino, ethylamino, iso-propylamino, and the like.
Some examples of substituents of the above-described aliphatic (and other) moieties include, but are not limited to aliphatic; heteroaliphatic; aryl; heteroaryl; alkylaryl; alkylheteroaryl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; F; Cl; Br; I; —OH; —NO2; —CN; —CF3; —CH2CF3; —CHCl2; —CH2OH; —CH2CH2OH; —CH2NH2; —CH2SO2CH3; —C(O)Rx; —CO2(Rx); —CON(Rx)2; —OC(O)Rx; —OCO2Rx; —OCON(Rx)2; —N(Rx)2; —S(O)2Rx; —NRx(CO)Rx; wherein each occurrence of Rx independently includes, but is not limited to, aliphatic, alicyclic, heteroaliphatic, heterocyclic, aryl, heteroaryl, alkylaryl, or alkylheteroaryl, wherein any of the aliphatic, heteroaliphatic, alkylaryl, or alkylheteroaryl substituents described above and herein may be substituted or unsubstituted, branched or unbranched, cyclic or acyclic, and wherein any of the aryl or heteroaryl substituents described above and herein may be substituted or unsubstituted. Additional examples of generally applicable substituents are illustrated by the specific embodiments shown in the Examples that are described herein.
In general, the term “aryl,” as used herein, refers to a stable mono- or polycyclic, unsaturated moiety having preferably 3-14 carbon atoms, each of which may be substituted or unsubstituted. In certain embodiments, the term “aryl” refers to a planar ring having p-orbitals perpendicular to the plane of the ring at each ring atom and satisfying the Huckel rule where the number of pi electrons in the ring is (4n+2) wherein n is an integer. A mono- or polycyclic, unsaturated moiety that does not satisfy one or all of these criteria for aromaticity is defined herein as “non-aromatic”, and is encompassed by the term “alicyclic”.
In general, the term “heteroaryl,” as used herein, refers to a stable mono- or polycyclic, unsaturated moiety having preferably 3-14 carbon atoms, each of which may be substituted or unsubstituted; and comprising at least one heteroatom selected from O, S, and N within the ring (i.e., in place of a ring carbon atom). In certain embodiments, the term “heteroaryl” refers to a planar ring comprising at least one heteroatom, having p-orbitals perpendicular to the plane of the ring at each ring atom, and satisfying the Huckel rule where the number of pi electrons in the ring is (4n+2) wherein n is an integer.
It will also be appreciated that aryl and heteroaryl moieties, as defined herein may be attached via an alkyl or heteroalkyl moiety and thus also include -(alkyl)aryl, -(heteroalkyl)aryl, -(heteroalkyl)heteroaryl, and -(heteroalkyl)heteroaryl moieties. Thus, as used herein, the phrases “aryl or heteroaryl moieties” and “aryl, heteroaryl, -(alkyl)aryl, -(heteroalkyl)aryl, -(heteroalkyl)heteroaryl, and -(heteroalkyl)heteroaryl” are interchangeable. Substituents include, but are not limited to, any of the previously mentioned substituents, i.e., the substituents recited for aliphatic moieties, or for other moieties as disclosed herein, resulting in the formation of a stable compound.
The term “aryl,” as used herein, does not differ significantly from the common meaning of the term in the art and refers to an unsaturated cyclic moiety comprising at least one aromatic ring. In certain embodiments, “aryl” refers to a mono- or bicyclic carbocyclic ring system having one or two aromatic rings including, but not limited to, phenyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl, and the like.
The term “heteroaryl,” as used herein, does not differ significantly from the common meaning of the term in the art and refers to a cyclic aromatic radical having from five to ten ring atoms of which one ring atom is selected from S, O, and N; zero, one or two ring atoms are additional heteroatoms independently selected from S, O, and N; and the remaining ring atoms are carbon, the radical being joined to the rest of the molecule via any of the ring atoms, such as, for example, pyridyl, pyrazinyl, pyrimidinyl, pyrrolyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isooxazolyl, thiadiazolyl, oxadiazolyl, thiophenyl, furanyl, quinolinyl, isoquinolinyl, and the like.
It will be appreciated that aryl and heteroaryl groups (including bicyclic aryl groups) can be unsubstituted or substituted, wherein substitution includes replacement of one or more of the hydrogen atoms thereon independently with any one or more of the following moieties including, but not limited to: aliphatic; alicyclic; heteroaliphatic; heterocyclic; aromatic; heteroaromatic; aryl; heteroaryl; alkylaryl; heteroalkylaryl; alkylheteroaryl; heteroalkylheteroaryl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; F; Cl; Br; I; —OH; —NO2; —CN; —CF3; —CH2CF3; —CHCl2; —CH2OH; —CH2CH2OH; —CH2NH2; —CH2SO2CH3; —C(O)Rx; —CO2(Rx); —CON(Rx)2; —OC(O)Rx; —OCO2Rx; —OCON(Rx)2; —N(Rx)2; —S(O)Rx; —S(O)2Rx; —NRx(CO)Rx wherein each occurrence of Rx independently includes, but is not limited to, aliphatic, alicyclic, heteroaliphatic, heterocyclic, aromatic, heteroaromatic, aryl, heteroaryl, alkylaryl, alkylheteroaryl, heteroalkylaryl, or heteroalkylheteroaryl, wherein any of the aliphatic, alicyclic, heteroaliphatic, heterocyclic, alkylaryl, or alkylheteroaryl substituents described above and herein may be substituted or unsubstituted, branched or unbranched, saturated or unsaturated, and wherein any of the aromatic, heteroaromatic, aryl, heteroaryl, -(alkyl)aryl or -(alkyl)heteroaryl substituents described above and herein may be substituted or unsubstituted. Additionally, it will be appreciated that any two adjacent groups taken together may represent a 4, 5, 6, or 7-membered substituted or unsubstituted alicyclic or heterocyclic moiety. Additional examples of generally applicable substituents are illustrated by the specific embodiments described herein.
The term “cycloalkyl,” as used herein, refers specifically to groups having three to seven, preferably three to ten, carbon atoms. Suitable cycloalkyls include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and the like, which, as in the case of aliphatic, alicyclic, heteroaliphatic or heterocyclic moieties, may optionally be substituted with substituents including, but not limited to aliphatic; alicyclic; heteroaliphatic; heterocyclic; aromatic; heteroaromatic; aryl; heteroaryl; alkylaryl; heteroalkylaryl; alkylheteroaryl; heteroalkylheteroaryl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; F; Cl; Br; I; —OH; —NO2; —CN; —CF3; —CH2CF3; —CHCl2; —CH2OH; —CH2CH2OH; —CH2NH2; —CH2SO2CH3; —C(O)Rx; —CO2(Rx); —CON(Rx)2; —OC(O)Rx; —OCO2Rx; —OCON(Rx)2; —N(Rx)2; —S(O)2Rx; —NRx(CO)Rx; wherein each occurrence of Rx independently includes, but is not limited to, aliphatic, alicyclic, heteroaliphatic, heterocyclic, aromatic, heteroaromatic, aryl, heteroaryl, alkylaryl, alkylheteroaryl, heteroalkylaryl or heteroalkylheteroaryl, wherein any of the aliphatic, alicyclic, heteroaliphatic, heterocyclic, alkylaryl, or alkylheteroaryl substituents described above and herein may be substituted or unsubstituted, branched or unbranched, saturated or unsaturated, and wherein any of the aromatic, heteroaromatic, aryl or heteroaryl substituents described above and herein may be substituted or unsubstituted. Additional examples of generally applicable substituents are illustrated by the specific embodiments shown in the Examples that are described herein.
The term “heteroaliphatic,” as used herein, refers to aliphatic moieties in which one or more carbon atoms in the main chain have been substituted with a heteroatom. Thus, a heteroaliphatic group refers to an aliphatic chain which contains one or more oxygen, sulfur, nitrogen, phosphorus or silicon atoms, e.g., in place of carbon atoms. Heteroaliphatic moieties may be linear or branched, and saturated or unsaturated. In certain embodiments, heteroaliphatic moieties are substituted by independent replacement of one or more of the hydrogen atoms thereon with one or more moieties including, but not limited, to aliphatic; alicyclic; heteroaliphatic; heterocyclic; aromatic; heteroaromatic; aryl; heteroaryl; alkylaryl; alkylheteroaryl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; F; Cl; Br; I; —OH; —NO2; —CN; —CF3; —CH2CF3; —CHCl2; —CH2OH; —CH2CH2OH; —CH2NH2; —CH2SO2CH3; —C(O)Rx; —CO2(Rx); —CON(Rx)2; —OC(O)Rx; —OCO2Rx; —OCON(Rx)2; —N(Rx)2; —S(O)2Rx; —NRx(CO)Rx, wherein each occurrence of Rx independently includes, but is not limited to, aliphatic, alicyclic, heteroaliphatic, heterocyclic, aromatic, heteroaromatic, aryl, heteroaryl, alkylaryl, alkylheteroaryl, heteroalkylaryl or heteroalkylheteroaryl, wherein any of the aliphatic, alicyclic, heteroaliphatic, heterocyclic, alkylaryl, or alkylheteroaryl substituents described above and herein may be substituted or unsubstituted, branched or unbranched, saturated or unsaturated, and wherein any of the aromatic, heteroaromatic, aryl or heteroaryl substituents described above and herein may be substituted or unsubstituted. Additional examples of generally applicable substituents are illustrated by the specific embodiments described herein.
The term “heterocycloalkyl,” “heterocycle,” or “heterocyclic,” as used herein, refers to compounds which combine the properties of heteroaliphatic and cyclic compounds and include, but are not limited to, saturated and unsaturated mono- or polycyclic cyclic ring systems having 5-16 atoms wherein at least one ring atom is a heteroatom selected from O, S, and N (wherein the nitrogen or sulfur heteroatom may optionally be oxidized), wherein the ring systems are optionally substituted with one or more functional groups, as defined herein. In certain embodiments, the term “heterocycloalkyl”, “heterocycle” or “heterocyclic” refers to a non-aromatic 5-, 6- or 7-membered ring or a polycyclic group wherein at least one ring atom is a heteroatom selected from O, S, and N (wherein the nitrogen and sulfur heteroatoms may be optionally be oxidized), including, but not limited to, a bi- or tri-cyclic group, comprising fused six-membered rings having between one and three heteroatoms independently selected from oxygen, sulfur and nitrogen, wherein (i) each 5-membered ring has 0 to 2 double bonds, each 6-membered ring has 0 to 2 double bonds and each 7-membered ring has 0 to 3 double bonds, (ii) the nitrogen and sulfur heteroatoms may be optionally be oxidized, (iii) the nitrogen heteroatom may optionally be quaternized, and (iv) any of the above heterocyclic rings may be fused to an aryl or heteroaryl ring. Representative heterocycles include, but are not limited to, heterocycles such as furanyl, thiofuranyl, pyranyl, pyrrolyl, thienyl, pyrrolidinyl, pyrazolinyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, piperidinyl, piperazinyl, oxazolyl, oxazolidinyl, isooxazolyl, isoxazolidinyl, dioxazolyl, thiadiazolyl, oxadiazolyl, tetrazolyl, triazolyl, thiatriazolyl, oxatriazolyl, thiadiazolyl, oxadiazolyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, dithiazolyl, dithiazolidinyl, tetrahydrofuryl, and benzofused derivatives thereof. In certain embodiments, a “substituted heterocycle, or heterocycloalkyl or heterocyclic” group is utilized and as used herein, refers to a heterocycle, or heterocycloalkyl or heterocyclic group, as defined above, substituted by the independent replacement of one, two, or three of the hydrogen atoms thereon with, but are not limited to, aliphatic; alicyclic; heteroaliphatic; heterocyclic; aromatic; heteroaromatic; aryl; heteroaryl; alkylaryl; heteroalkylaryl; alkylheteroaryl; heteroalkylheteroaryl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; F; Cl; Br; I; —OH; —NO2; —CN; —CF3; —CH2CF3; —CHCl2; —CH2OH; —CH2CH2OH; —CH2NH2; —CH2SO2CH3; —C(O)Rx; —CO2(Rx); —CON(Rx)2; —OC(O)Rx; —OCO2Rx; —OCON(Rx)2; —N(Rx)2; —S(O)2Rx; —NRx(CO)Rx wherein each occurrence of Rx independently includes, but is not limited to, aliphatic, alicyclic, heteroaliphatic, heterocyclic, aromatic, heteroaromatic, aryl, heteroaryl, alkylaryl, alkylheteroaryl, heteroalkylaryl or heteroalkylheteroaryl, wherein any of the aliphatic, alicyclic, heteroaliphatic, heterocyclic, alkylaryl, or alkylheteroaryl substituents described above and herein may be substituted or unsubstituted, branched or unbranched, saturated or unsaturated, and wherein any of the aromatic, heteroaromatic, aryl or heteroaryl substitutents described above and herein may be substituted or unsubstituted. Additional examples or generally applicable substituents are illustrated by the specific embodiments described herein.
The terms “carbohydrate” as used herein refers to compounds of the general molecular formula CnH2nOn. Most carbohydrates are aldehydes or ketones with multiple hydroxyl groups, usually one on each carbon atom of the molecule. A carbohydrate may be a monosaccharide, disaccharide, trisaccharide, oligosaccharide, or polysaccharide. The most basic carbohydrate is a monosaccharide, such as glucose, sucrose, galactose, mannose, ribose, arabinose, xylose, or fructose. Disaccharides are two joined monosaccharides. Exemplary disaccharides include sucrose, maltose, cellobiose, and lactose. Typically, an oligosaccharide includes between three and six monosaccharide units (e.g., raffinose, stachyose), and polysaccharides include six or more monosaccharide units. Exemplary polysaccharides include starch, glycogen, and cellulose. Carbohydrates may contain modified saccharide units such as 2′-deoxyribose wherein a hydroxyl group is removed, 2′-fluororibose wherein a hydroxyl group is replaced with a fluorine, or N-acetylglucosamine, a nitrogen-containing form of glucose (e.g., 2′-fluororibose, deoxyribose, and hexose). Carbohydrates may exist in many different forms, for example, conformers, cyclic forms, acyclic forms, stereoisomers, tautomers, anomers, and isomers.
Additionally, it will be appreciated that any of the alicyclic or heterocyclic moieties described above and herein may comprise an aryl or heteroaryl moiety fused thereto. Additional examples of generally applicable substituents are illustrated by the specific embodiments described herein. The terms “halo” and “halogen” as used herein refer to an atom selected from fluorine, chlorine, bromine, and iodine.
The terms “halo” and “halogen” as used herein refer to an atom selected from fluorine, chlorine, bromine, and iodine.
The term “haloalkyl” denotes an alkyl group, as defined above, having one, two, or three halogen atoms attached thereto and is exemplified by such groups as chloromethyl, bromoethyl, trifluoromethyl, and the like.
The term “amino,” as used herein, refers to a primary (—NH2), secondary (—NHRx, tertiary (—NRxRy), or quaternary (—N+RxRyRz) amine, where Rx, Ry and Rz, are independently an aliphatic, alicyclic, heteroaliphatic, heterocyclic, aryl, or heteroaryl moiety, as defined herein. Examples of amino groups include, but are not limited to, methylamino, dimethylamino, ethylamino, diethylamino, diethylaminocarbonyl, methylethylamino, iso-propylamino, piperidino, trimethylamino, and propylamino.
The term “alkylidene,” as used herein, refers to a substituted or unsubstituted, linear or branched saturated divalent radical consisting solely of carbon and hydrogen atoms, having from one to n carbon atoms, having a free valence “-” at both ends of the radical. In certain embodiments, the alkylidene moiety has 1 to 6 carbon atoms.
The term “alkenylidene,” as used herein, refers to a substituted or unsubstituted, linear or branched unsaturated divalent radical consisting solely of carbon and hydrogen atoms, having from two to n carbon atoms, having a free valence “-” at both ends of the radical, and wherein the unsaturation is present only as double bonds and wherein a double bond can exist between the first carbon of the chain and the rest of the molecule. In certain embodiments, the alkenylidene moiety has 2 to 6 carbon atoms.
The term “alkynylidene,” as used herein, refers to a substituted or unsubstituted, linear or branched unsaturated divalent radical consisting solely of carbon and hydrogen atoms, having from two to n carbon atoms, having a free valence “-” at both ends of the radical, and wherein the unsaturation is present only as triple or double bonds and wherein a triple or double bond can exist between the first carbon of the chain and the rest of the molecule. In certain embodiments, the alkynylidene moiety has 2 to 6 carbon atoms.
Unless otherwise indicated, as used herein, the terms “alkyl”, “alkenyl”, “alkynyl”, “heteroalkyl”, “heteroalkenyl”, “heteroalkynyl”, “alkylidene”, alkenylidene”, -(alkyl)aryl, -(heteroalkyl)aryl, -(heteroalkyl)aryl, -(heteroalkyl)heteroaryl, and the like encompass substituted and unsubstituted, and linear and branched groups. Similarly, the terms “aliphatic”, “heteroaliphatic”, and the like encompass substituted and unsubstituted, saturated and unsaturated, and linear and branched groups. Similarly, the terms “cycloalkyl”, “heterocycle”, “heterocyclic”, and the like encompass substituted and unsubstituted, and saturated and unsaturated groups. Additionally, the terms “cycloalkenyl”, “cycloalkynyl”, “heterocycloalkenyl”, “heterocycloalkynyl”, “aromatic”, “heteroaromatic, “aryl”, “heteroaryl” and the like encompass both substituted and unsubstituted groups.
The phrase, “pharmaceutically acceptable derivative,” as used herein, denotes any pharmaceutically acceptable salt, ester, or salt of such ester, of such compound, or any other adduct or derivative which, upon administration to a patient, is capable of providing (directly or indirectly) a compound as otherwise described herein, or a metabolite or residue thereof. Pharmaceutically acceptable derivatives thus include among others pro-drugs. A pro-drug is a derivative of a compound, usually with significantly reduced pharmacological activity, which contains an additional moiety, which is susceptible to removal in vivo yielding the parent molecule as the pharmacologically active species. An example of a pro-drug is an ester, which is cleaved in vivo to yield a compound of interest. Pro-drugs of a variety of compounds, and materials and methods for derivatizing the parent compounds to create the pro-drugs, are known and may be adapted to the present invention. The biological activity of pro-drugs and pro-drugs may also be altered by appending a functionality onto the compound, which may be catalyzed by an enzyme. Also, included are oxidation and reduction reactions, including enzyme-catalyzed oxidation and reduction reactions. Certain exemplary pharmaceutical compositions and pharmaceutically acceptable derivatives will be discussed in more detail herein below.
By the term “protecting group,” as used herein, it is meant that a particular functional moiety, e.g., O, S, or N, is temporarily blocked so that a reaction can be carried out selectively at another reactive site in a multifunctional compound. In certain embodiments, a protecting group reacts selectively in good yield to give a protected substrate that is stable to the projected reactions; the protecting group must be selectively removed in good yield by readily available, preferably nontoxic reagents that do not attack the other functional groups; the protecting group forms an easily separable derivative (more preferably without the generation of new stereogenic centers); and the protecting group has a minimum of additional functionality to avoid further sites of reaction. As detailed herein, oxygen, sulfur, nitrogen, and carbon protecting groups may be utilized. For example, in certain embodiments, as detailed herein, certain exemplary oxygen protecting groups are utilized. These oxygen protecting groups include, but are not limited to methyl ethers, substituted methyl ethers (e.g., MOM (methoxymethyl ether), MTM (methylthiomethyl ether), BOM (benzyloxymethyl ether), PMBM or MPM (p-methoxybenzyloxymethyl ether), to name a few), substituted ethyl ethers, substituted benzyl ethers, silyl ethers (e.g., TMS (trimethylsilyl ether), TES (triethylsilylether), TIPS (triisopropylsilyl ether), TBDMS (t-butyldimethylsilyl ether), tribenzyl silyl ether, TBDPS (t-butyldiphenyl silyl ether), to name a few), esters (e.g., formate, acetate, benzoate (Bz), trifluoroacetate, dichloroacetate, to name a few), carbonates, cyclic acetals and ketals. In certain other exemplary embodiments, nitrogen protecting groups are utilized. These nitrogen protecting groups include, but are not limited to, carbamates (including methyl, ethyl and substituted ethyl carbamates (e.g., Troc), to name a few) amides, cyclic imide derivatives, N-Alkyl and N-Aryl amines, imine derivatives, and enamine derivatives, to name a few. Certain other exemplary protecting groups are detailed herein, however, it will be appreciated that the present invention is not intended to be limited to these protecting groups; rather, a variety of additional equivalent protecting groups can be readily identified using the above criteria and utilized in the present invention. Additionally, a variety of protecting groups are described in Protective Groups in Organic Synthesis, Third Ed. Greene, T. W. and Wuts, P. G., Eds., John Wiley & Sons, New York: 1999, the entire contents of which are hereby incorporated by reference.
As used herein, the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N+(C1-4alkyl)4 salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Other pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate, and aryl sulfonate.
Additionally, as used herein, the term “pharmaceutically acceptable ester” refers to esters that hydrolyze in vivo and include those that break down readily in the human body to leave the parent compound or a salt thereof. Suitable ester groups include, for example, those derived from pharmaceutically acceptable aliphatic carboxylic acids, particularly alkanoic, alkenoic, cycloalkanoic and alkanedioic acids, in which each alkyl or alkenyl moeity advantageously has not more than 6 carbon atoms. Examples of particular esters include formates, acetates, propionates, butyrates, acrylates and ethylsuccinates.
Furthermore, the term “pharmaceutically acceptable prodrugs” as used herein refers to those prodrugs of the compounds of the present invention which are, within the scope of sound medical judgment, suitable for use in contact with the issues of humans and lower animals with undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio, and effective for their intended use, as well as the zwitterionic forms, where possible, of the compounds of the invention. The term “prodrug” refers to compounds that are rapidly transformed in vivo to yield the parent compound of the above formula, for example by hydrolysis in blood. A thorough discussion is provided in T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems, vol. 14 of the A.C.S. Symposium Series, and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference.
As used herein, the term “tautomer” includes two or more interconvertable compounds resulting from at least one formal migration of a hydrogen atom and at least one change in valency (e.g., a single bond to a double bond, a triple bond to a single bond, or vice versa). The exact ratio of the tautomers depends on several factors, including temperature, solvent, and pH. Tautomerizations (i.e., the reaction providing a tautomeric pair) may catalyzed by acid or base. Exemplary tautomerizations include keto-to-enol; amide-to-imide; lactam-to-lactim; enamine-to-imine; and enamine-to-(a different) enamine tautomerizations.
As used herein, the term “isomers” includes any and all geometric isomers and stereoisomers. For example, “isomers” include cis- and trans-isomers, E- and Z-isomers, R- and S-enantiomers, diastereomers, (
As used herein, the term “small molecule” refers to a non-peptidic, non-oligomeric organic compound either synthesized in the laboratory or found in nature. Small molecules, as used herein, can refer to compounds that are “natural product-like”, however, the term “small molecule” is not limited to “natural product-like” compounds. Rather, a small molecule is typically characterized in that it contains several carbon-carbon bonds, and has a molecular weight of less than 2000 g/mol, preferably less than 1500 g/mol, although this characterization is not intended to be limiting for the purposes of the present invention. Examples of “small molecules” that occur in nature include, but are not limited to, taxol, dynemicin, and rapamycin. Examples of “small molecules” that are synthesized in the laboratory include, but are not limited to, compounds described in Tan et al., (“Stereoselective Synthesis of over Two Million Compounds Having Structural Features Both Reminiscent of Natural Products and Compatible with Miniaturized Cell-Based Assays” J. Am. Chem. Soc. 120:8565, 1998; incorporated herein by reference). In certain other embodiments, natural-product-like small molecules are utilized.
As used herein the term “biological sample” includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from an animal (e.g., mammal) or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof. For example, the term “biological sample” refers to any solid or fluid sample obtained from, excreted by or secreted by any living organism, including single-celled micro-organisms (such as bacteria and yeasts) and multicellular organisms (such as plants and animals, for instance a vertebrate or a mammal, and in particular a healthy or apparently healthy human subject or a human subject affected by a condition or disease to be diagnosed or investigated).
The biological sample can be in any form, including a solid material such as a tissue, cells, a cell pellet, a cell extract, cell homogenates, or cell fractions; or a biopsy, or a biological fluid. The biological fluid may be obtained from any site (e.g., blood, saliva (or a mouth wash containing buccal cells), tears, plasma, serum, urine, bile, cerebrospinal fluid, amniotic fluid, peritoneal fluid, and pleural fluid, or cells therefrom, aqueous or vitreous humor, or any bodily secretion), a transudate, an exudate (e.g., fluid obtained from an abscess or any other site of infection or inflammation), or fluid obtained from a joint (e.g., a normal joint or a joint affected by disease such as rheumatoid arthritis, osteoarthritis, gout or septic arthritis).
The biological sample can be obtained from any organ or tissue (including a biopsy or autopsy specimen) or may comprise cells (whether primary cells or cultured cells) or medium conditioned by any cell, tissue or organ. Biological samples may also include sections of tissues such as frozen sections taken for histological purposes. Biological samples also include mixtures of biological molecules including proteins, lipids, carbohydrates, and nucleic acids generated by partial or complete fractionation of cell or tissue homogenates. Although the sample is preferably taken from a human subject, biological samples may be from any animal, plant, bacteria, virus, yeast, etc. If desired, the biological sample may be subjected to preliminary processing, including preliminary separation techniques.
The term animal, as used herein, refers to humans as well as non-human animals, at any stage of development, including, for example, mammals, birds, reptiles, amphibians, fish, worms and single cells. In certain embodiments, the non-human animal is a mammal (e.g., a rodent, a mouse, a rat, a rabbit, a monkey, a dog, a cat, a sheep, cattle, a primate, or a pig). An animal may be a transgenic animal.
Ocular cancer can be a difficult and devastating disease to treat, often resulting in the partial or total loss of vision due to enucleation or other therapies. Retinoblastoma is the most common childhood primary intraocular malignancy and affects approximately 5,000 to 8,000 children worldwide each year. Until the 1990's, enucleation and/or external beam radiotherapy (EBRT) were the standards of care for children with advanced bilateral disease. However, an increased incidence of secondary malignancies as well as disfigurement caused by orbital hypoplasia associated with scatter doses of radiation has spurred investigators to seek alternative forms of treatment. Accordingly, regimens incorporating systemic chemotherapy as a means of shrinking the tumor prior to focal treatments has replaced EBRT as the primary treatment for retinoblastoma. Unfortunately, the severe toxicity associated with systemic chemotherapy, especially in children under the age of one, limits the efficacy of this method of treatment and has led to the development of improved approaches for treating patients with retinoblastoma. One such approach involves localized delivery of a chemotherapeutic agent via direct intraarterial infusion into the ophthalmic artery. Direct intraarterial infusion, when applied to the treatment of retinoblastoma, involves the delivery of high-dose chemotherapy via ophthalmic artery infusion. This prevents the drug from entering the systemic bloodstream, substantially reducing systemic toxicity. Thus, direct intraarterial infusion through the ophthalmic artery allows for higher doses of chemotherapeutic agents for ocular cancers.
The present invention is based on a chemical screen aimed at identifying alternative chemotherapeutic agents for local delivery in the treatment of retinoblastoma. Many of the compounds identified herein are cardenolides or derivatives thereof. The activity of cardenolides against ocular cancers first emerged in a previous investigation in which a library of known compounds were screened to assess their utility as chemotherapeutic agents to be administered locally to a subject (e.g., to an eye with cancer). Cardenolides are currently used in the clinic for the treatment of heart disease and have well-characterized and well-defined pharmacological profiles. Thus, they provide an attractive scaffold for synthesizing novel cardenolides, derivatives thereof, and other structurally related compounds for use in the treatment of retinoblastoma, particularly via local administration of the compound or compounds into the ophthalmic artery.
In part, the present invention encompasses the recognition that an inventive compound of the formulae shown below may be used to treat an ocular cancer when administered locally to the eye of a subject with an ocular cancer. In one aspect, the present invention provides novel compounds that may be used to treat an ocular cancer (e.g., retinoblastoma). In certain embodiments, inventive compounds described herein are of the formula:
wherein:
the dashed line between C4 and C5 indicates that the bond between C4 and C5 is either a single or a double bond; and wherein the dashed line between C4 and C5 cannot be a double bond if the bond between C5 and C6 is a double bond.
the dashed line between C5 and C6 indicates that the bond between C5 and C6 is either a single or a double bond; and wherein the dashed line between C5 and C6 cannot be a double bond if the bond between C4 and C5 is a double bond.
the dashed line between C16 and C17 indicates that the bond between C16 and C17 is either a single or a double bond;
R1 is
R2 is H, OH, OAc, or ORb, wherein Rb is a suitable protecting group;
R3 is H, OH, OAc, or ORc, wherein Rc is a suitable protecting group;
R4 is H, OH, OAc, or ORd, wherein Rd is a suitable protecting group;
R5 is H, C1-6 alkyl, OH, OAc, or ORe, wherein Re is a suitable protecting group;
wherein R4 and R5 are optionally taken together to form an epoxide;
R6 is H, OH, OAc, or ORf, wherein Rf is a suitable protecting group; and wherein L is an integer from 0 to 1;
R7 is H, C1-6 alkyl, OH, OAc, or ORg, wherein Rg is a carbohydrate moiety or a suitable protecting group;
R8 is H, OH, OAc, C1-6 alkyl, CHO, CH2OH, CH2ORh, CO2H, CO2Rh or ORh, wherein Rh is a suitable protecting group; and
R9 is H, OH, OAc, or ORi, wherein Ri is a suitable protecting group;
or a pharmaceutically acceptable salt thereof.
In certain embodiments, the dashed line between C5 and C6 is a single bond. In certain embodiments, the dashed line between C5 and C6 is a double bond. In certain embodiments, the dashed line between C16 and C17 is a single bond. In certain embodiments, the dashed line between C16 and C17 is a double bond. In certain embodiments, the dashed line between C5 and C6 is a single bond and the dashed line between C16 and C17 is a single bond.
In certain embodiments, R1 is
In some embodiments R1 is
In some embodiments, R2 is H. In certain embodiments, R2 is OH. In certain embodiments, R2 is OAc. In certain embodiments, R2 is ORb, wherein Rb is a suitable protecting group.
In some embodiments, R3 is H. In some embodiments, R3 is OH. In some embodiments, R3 is OAc. In some embodiments, R3 is ORc, wherein Rc is a suitable protecting group
In some embodiments, R4 is H. In some embodiments, R4 is OH. In some embodiments, R4 is ORd, wherein Rd is a suitable protecting group. In certain embodiments, R4 is optionally taken together with R5 to form an epoxide.
In some embodiments, R5 is H. In some embodiments, R5 is methyl. In some embodiments, R5 is ethyl. In some embodiments, R5 is propyl. In some embodiments, R5 is butyl. In some embodiments, R5 is pentyl. In some embodiments, R5 is hexyl. In some embodiments, R5 is OH. In some embodiments, R5 is ORe, wherein Re is a suitable protecting group. In certain embodiments, R5 is optionally taken together with R4 to form an epoxide.
In some embodiments, R6 is H. In some embodiments, R6 is OH. In some embodiments, R6 is ORf, wherein Rf is a suitable protecting group.
In certain embodiments, L is 0. In certain embodiments, L is 1.
In certain embodiments, R7 is H. In some embodiments, R7 is methyl. In some embodiments, R7 is ethyl. In some embodiments, R7 is propyl. In some embodiments, R7 is butyl. In some embodiments, R7 is pentyl. In some embodiments, R7 is hexyl. In some embodiments, R7 is OH or ORg, wherein Rg is a suitable protecting group. In some embodiments, Rg is a carbohydrate moiety such as, for instance, a glycoside. In certain embodiments, Rg is a starch. In certain embodiments, Rg is glycogen. In certain embodiments, Rg is dextran. In certain embodiments, Rg is cyclodextran. In certain embodiments, Rg is a hyaluranic acid. In some embodiments, Rg is an oligosaccharide. In certain embodiments, Rg is a disaccharide. In certain embodiments, Rg is sucrose. In certain embodiments, Rg is lactose. In certain embodiments, Rg is maltose. In some embodiments, Rg is a monosaccharide. In certain embodiments, Rg is glucose. In certain embodiments, Rg is fructose. In certain embodiments, Rg is galactose. In certain embodiments, Rg is mannose. In certain embodiments, Rg is xylose. In certain embodiments, Rg is ribose. In some embodiments, Rg is a carbohydrate derivative (e.g., an ester, ether, aminated, amidated, sulfated, phosphosubstituted, or otherwise suitably protected carbohydrate).
In certain embodiments, Rg is of the formulae:
In some embodiments, R8 is H. In some embodiments, R8 is OH. In some embodiments, R8 is OAc. In some embodiments, R8 is methyl. In some embodiments, R8 is ethyl. In some embodiments, R8 is propyl. In some embodiments, R8 is butyl. In some embodiments, R8 is pentyl. In some embodiments, R8 is hexyl. In some embodiments, R8 is CHO. In some embodiments, R8 is CH2OH. In some embodiments, R9 is CH2ORh. In some embodiments, R8 is CO2H. In some embodiments, R8 is CO2Rh. In some embodiments, R8 is ORh, wherein Rh is a suitable protecting group.
In some embodiments, R9 is H. In some embodiments, R9 is OH. In some embodiments, R9 is OAc. In some embodiments, R9 is ORi, wherein Ri; is a suitable protecting group.
In certain embodiments, the compound is of the formula:
wherein R2, R3, R4, R5, R6, R8, R9, L, Rg, and the dashed lines are as defined herein; and pharmaceutically acceptable salts thereof. In some embodiments, the dashed line represents a double bond, and L is 0. In some embodiments, the dashed line represents a single bond, and L is 1.
In certain embodiments, the compound is of the formulae:
wherein R3, R4, R5, R6, R8, R9, L, Rg, and the dashed lines are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
wherein R3, R4, R5, R6, R8, R9, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
wherein R4, R5, R6, R8, R9, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
wherein R3, R4, R5, R6, R8, R9, and Rg, are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
wherein R3, R4, R5, R6, R8, R9, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
wherein R3, R4, R5, R6, R8, R9, and Rg, are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
wherein R2, R3, R5, R6, R8, R9, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof. In certain embodiments, the compound is as defined herein and Rg is a carbohydrate moiety.
In certain embodiments, the compound is of the formulae:
wherein R2, R3, R5, R6, R9, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
wherein R2, R3, R5, R6, R8, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula: In certain embodiments, the compound is of the formula:
wherein R2, R3, R4, R5, R6, R8, R9, L, Rg, and the dashed lines are as defined herein; and pharmaceutically acceptable salts thereof. In some embodiments, the dashed line represents a double bond, and L is 0. In some embodiments, the dashed line represents a single bond, and L is 1.
In certain embodiments, the compound If of the formula:
In certain embodiments, the compound is of the formula:
In certain embodiments, the compound is of the formula:
In certain embodiments, the compound is of the formula:
In certain embodiments, the compound is of the formula:
In certain embodiments, the compound is of the formula:
wherein R7, R8, L, Rg, and the dashed line are as defined herein; and pharmaceutically acceptable salts thereof. In some embodiments, the compound is as shown above and Rg is:
In certain embodiments, the compound is of the formula:
wherein R8 and Rg are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
wherein R8 is as defined herein and Rg is a carbohydrate; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
wherein R8 is as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
and pharmaceutically acceptable salts thereof.
In other embodiments, the compound of the invention is of the formula:
wherein:
R1 is
R2 is H, OH, OAc, or ORb, wherein Rb is a suitable protecting group;
R3 is H, OH, OAc, or ORc, wherein Rc is a suitable protecting group;
R4 is H, C1-6 alkyl, OH, OAc or ORd, wherein Rd is a suitable protecting group;
R5 is H, OH, OAc, or ORe, wherein Re is a suitable protecting group;
R6 is H, OH, OAc, or ORf, wherein Rf is a suitable protecting group;
R7 is H, C1-6 alkyl, CHO, CH2OH, CH2ORg, CO2H, or CO2Rg wherein Rg is a suitable protecting group; and
R8 is H or C1-6 alkyl; or a pharmaceutically acceptable salt thereof.
In some embodiments, R1 is:
In some embodiments, R1 is:
In some embodiments, R2 is H. In some embodiments, R2 is OH. In some embodiments, R2 is OAc. In some embodiments, R2 is ORb, wherein Rb is a suitable protecting group.
In some embodiments, R3 is H. In some embodiments, R3 is OH. In some embodiments, R3 is OAc. In some embodiments, R3 is ORc, wherein Rc is a suitable protecting group.
In some embodiments, R4 is H. In some embodiments, R4 is methyl. In some embodiments, R4 is ethyl. In some embodiments, R4 is propyl. In some embodiments, R4 is butyl. In some embodiments, R4 is pentyl. In some embodiments, R4 is hexyl. In some embodiments, R4 is OH. In some embodiments, R4 is OAc. In some embodiments, R4 is ORd, wherein Rd is a suitable protecting group, R5 is H. In some embodiments, R5 is OH. In some embodiments, R5 is OAc. In some embodiments, R5 is or ORe wherein Re is a suitable protecting group.
In some embodiments, R6 is H. In some embodiments, R6 is OH. In some embodiments, R6 is OAc. In some embodiments, R6 is ORf wherein Rf is a suitable protecting group.
In some embodiments, R7 is H. In some embodiments, R7 is methyl. In some embodiments, R7 is ethyl. In some embodiments, R7 is propyl. In some embodiments, R7 Is butyl. In some embodiments, R7 is pentyl. In some embodiments, R7 is hexyl. In some embodiments, R7 is CHO. In some embodiments, R7 is CH2OH. In some embodiments, R7 is or CH2ORg. In some embodiments, R7 is CO2H. In some embodiments, R7 is CO2Rg, wherein Rg is a suitable protecting group. In some embodiments, R8 is H. In some embodiments, R8 is methyl. In some embodiments, R8 is ethyl. In some embodiments, R8 is propyl. In some embodiments, R8 Is butyl. In some embodiments, R8 is pentyl. In some embodiments, R8 is hexyl.
In certain embodiments, the compound has the stereochemistry of the formula:
wherein R1, R2, R3, R4, R5, R6, R7, and R8 are as defined herein; and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formula:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is of the formulae:
and pharmaceutically acceptable salts thereof.
In certain embodiments, the compound is any one of the following:
and pharmaceutically acceptable salts thereof.
Some of the foregoing compounds include one or more asymmetric centers, and thus can exist in various isomeric forms, e.g., stereoisomers and/or diastereomers. Thus, compounds useful in the present invention and pharmaceutical compositions thereof may be in the form of an individual enantiomer, diastereomer, or geometric isomer, or may be in the form of a mixture of stereoisomers. In certain embodiments, the compositions utilized in the invention include only one stereoisomer of a compound of the invention. In certain other embodiments, mixtures of stereoisomers or diastereomers are utilized.
The invention additionally encompasses the compounds as individual isomers substantially free of other isomers and alternatively, as mixtures of various isomers, e.g., racemic mixtures of stereoisomers. In addition to the compounds described herein, the invention also encompasses pharmaceutically acceptable derivatives of these compounds, and compositions comprising one or more inventive compounds and/or one or more pharmaceutically acceptable excipients.
Compounds utilized in the invention may be prepared by crystallization of the compound under different conditions and may exist as one or a combination of polymorphs of the compound. For example, different polymorphs may be identified and/or prepared using different solvents, or different mixtures of solvents for recrystallization; by performing crystallizations at different temperatures; or by using various modes of cooling, ranging from very fast to very slow cooling during crystallizations. Polymorphs may also be obtained by heating or melting the compound followed by gradual or fast cooling. The presence of polymorphs may be determined by solid probe NMR spectroscopy, IR spectroscopy, differential scanning calorimetry, powder X-ray diffractogram, and/or other techniques. Compounds utilized in the invention may also exist as amorphous compounds. The present invention encompasses the inventive compounds, their derivatives, their tautomers, their pro-drugs, their stereoisomers, their polymorphs, their pharmaceutically acceptable salts, their pharmaceutically acceptable solvates, their pharmaceutically acceptable hydrates, their pharmaceutically acceptable co-crystals, and pharmaceutically acceptable compositions thereof.
The instant invention provides methods of administering a therapeutically effective amount of an inventive compound to a subject in need thereof. In certain embodiments, the inventive method comprises administering a therapeutically effective amount of a compound of the invention locally to the eye of a subject with an ocular cancer. Local administration offers an improved approach to drug delivery because it allows the cytotoxic drug to bypass the systemic circulation and thereby minimizes the systemic toxicity of chemotherapeutic agents. Less systemic exposure allows for more effective local administration to the subject, potentially improving a subject's prognosis.
In certain embodiments, local administration is achieved via periocular administration using depots or implants that may produce high intraocular concentrations of the drug without the constraints of systemic toxicity. Prior studies with chemotherapeutics have shown, for example, that periocular administration can increase ocular concentrations by up to approximately 10-fold, compared to systemic administration (Mendelsohn et al., Archives of Ophthalmology 1998; 116:1209-1212). Such periocular implants could allow for more sustained exposure of the tumor to the drug.
Local administration of an inventive compound as described herein to the eye of a subject with ocular cancer allows for improved efficacy and/or decreased toxicity. In some embodiments, the subject being treated is a mammal. In some embodiments, the subject is a rodent. In some embodiments, the subject is a rat. In some embodiments, the subject is a mouse. In certain embodiments, the subject is a human. In certain embodiments, the subject is a human less than 18 years of age. In certain embodiments, the subject is a human less than 10 years of age. In certain embodiments, the subject is a human less than 5 years of age. In certain embodiments, the subject is a human less than 2 years of age. In certain embodiments, the subject is less than one year of age. In certain embodiments, the subject is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 years of age.
The ocular cancer being treated using the inventive treatment may be any one of a number of ocular cancers. In some embodiments, the ocular cancer is retinoblastoma. In certain embodiments, the ocular cancer is advanced retinoblastoma. In certain embodiments, the ocular cancer is advanced bilateral retinoblastoma. In certain embodiments, the retinoblastoma is intraocular retinoblastoma. In certain embodiments, the retinoblastoma is extraocular retinoblastoma. In certain embodiments, the retinoblastoma is recurrent retinoblastoma. In some embodiments, the cancer is medulloepithelioma. In some embodiments, the cancer is a ocular melanoma. In some embodiments, the cancer is lymphoma. In some embodiments, the cancer is a cancer that has metastasized to the eye.
The treatment may include a second therapy. The second therapy may be chemotherapy, radiotherapy, cryotherapy, external beam radiotherapy, thermotherapy, or brachytherapy. In certain embodiments, the second type of therapy used with local administration of a cardenolide is external beam radiotherapy. In certain embodiments, the second type of therapy is administration of a second chemotherapeutic agent.
The second chemotherapeutic agent may be a topoisomerase I inhibitor (e.g., topotecan), a topoisomerase II inhibitor (e.g., etoposide), a mitotic inhibitor (e.g., vincristine), an antimetabolite/thymidylate synthase inhibitor (e.g., 5-fluorouracil), a calcium channel blocker (e.g., verapamil), a nitrogen mustard alkylating agent (e.g., melphalan) or other alkylating agent (e.g., carboplatin, cisplatin), an MDM2 antagonist (e.g., Nutlin-3), an immunosuppressant (e.g., cyclosporin), a podophyllotoxin (e.g., teniposide), or a form of vitamin D3 (e.g., calcitriol). In certain embodiments, the second chemotherapeutic is an inhibitor of MDR1/Pgp. In some embodiments, the second chemotherapeutic agent is a multidrug resistance-associated protein-1 (MRP1) inhibitor or an ABC transporter inhibitor. In certain embodiments, the second chemotherapeutic agent is any one of those described herein and the inventive compound is any of the compounds described herein. In some embodiments, the second agent is delivered locally. In some embodiments, the second agent is delivered systemically.
In some embodiments, the inventive compound is administered locally to the site of the disease. Local administration may comprise local intraarterial infusion such as, for instance, direct intraarterial infusion into an artery that delivers blood to the diseased site. In some embodiments, when the disease is an ocular cancer, local administration comprises direct intraarterial infusion into the ophthalmic artery. In certain embodiments, direct intraarterial infusion into an ophthalmic artery of a subject with ocular cancer includes performing an arteriogram in order to visualize the vasculature around a desired artery, cathetering the desired artery, optionally performing a second angiogram to confirm that the desired artery vascularizes the diseased site, and infusing the artery with a therapeutically effective amount of chemotherapeutic agent, wherein the agent is a compound of the invention. In certain embodiments, a compound of the invention and carboplatin are administered as a combination therapy. In certain embodiments, and carboplatin are administered as a combination therapy.
The time of infusion ranges from approximately 1 minute to approximately 120 minutes. In some embodiments, the time of infusion ranges from approximately 1 minute to approximately 90 minutes. In some embodiments, the time of infusion ranges from approximately 1 minute to approximately 60 minutes. In some embodiments, the time of infusion ranges from approximately 5 minutes to approximately 45 minutes. In some embodiments, the time of infusion ranges from approximately 15 minutes to approximately 45 minutes. In some embodiments, the time of infusion is approximately 30 minutes. In certain embodiments, the treatment is repeated at least two times. In certain embodiments, the treatment is repeated at least three times. In certain embodiments, the treatment is repeated at least four times. In certain embodiments, the treatment is repeated 2, 3, 4, 5, 6, 7, 8, 9, or 10 times. In some embodiments, the treatment is repeated upon recurrence of the cancer. In certain embodiments, the treatment is repeated at least once a day. In certain embodiments, the treatment is repeated at least once every other day. In certain embodiments, the treatment is repeated at least once a week. In certain embodiments, the treatment is repeated at least twice a week. In certain embodiments, the treatment is repeated at least once a month. In certain embodiments, the treatment is repeated at least twice a month. In certain embodiments, the treatment is repeated at least three or four times a month. In certain embodiments, the treatment is repeated at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 times a month for as many months as necessary to achieve a desired outcome.
The efficacy of the inventive treatment may be evaluated using any method known in the art. The treatment of the cancer may be evaluated by physical examination, laboratory testing, imaging studies, electrophysiological studies, etc. Exemplary methods include external examination, visual acuity testing, pupil and motility evaluation, complete fundus examination under anesthesia including RetCam digital photography, and standard electroretinogram testing under photopic and scotopic conditions. In some embodiments, evaluation may include systemic evaluations comprising interval medical history, weight and height measurements, and complete blood counts. In some embodiments, the efficacy of the inventive treatment is evaluated using any combination of methods known in the medical arts.
Methods of assaying compounds are needed to determine an inventive compound's efficacy in treating different types of cancer. The inhibition of the growth of cells may be determined in vivo or in vitro. The instant invention provides methods of inhibiting the growth of ocular cells comprising contacting in vitro ocular cancer cells with an effective amount of a compound of the instant invention to inhibit the growth of cells. This method may comprise steps of contacting a compound of the instant invention with cells, and then incubating the cells under suitable conditions to test for the inhibition of growth. This method may comprise repeating these steps using various concentrations of the inventive compound to fully determine the extent of activity against cell growth.
The cells may be derived from a cancer cell line or a biological sample (e.g., a biopsy). Any cells found in the eye may be used in the inventive method. In certain embodiments, the cells are normal cells. In certain embodiments, the cells are cancer cells. In certain embodiments, the cancer cells are ocular cancer cells such as retinoblastoma cells. In certain embodiments, the ocular cancer cell line is the human retinoblastoma cell line Y79, WERI-Rb-1, RB355, or Y79LUC. In some embodiments, the ocular cancer cell line is a melanoma cell line. In certain embodiments, the ocular cancer is a uveal melanoma cell line such as C918 or Mum2b. In certain embodiments, the cells are derived from a biopsy of a patient with an ocular cancer. Such cells may be tested in vitro to determine the efficacy of a compound of the invention in treating the patient's cancer cells.
The instant invention also provides methods of identifying compounds that are useful in treating retinoblastoma, particularly those that may be useful in local delivery of a the compound to a diseased site. Such methods were used in a previous investigation to identify known compounds (e.g., cardenolides) that act as antiproliferative agents from a large library of known compounds as described below in the Examples. In certain embodiments, the invention provides methods of identifying inventive compounds which inhibit cellular proliferation. In certain embodiments, the invention provides methods of evaluating the potency of a test compound against a particular type of cell. In some embodiments, the invention provides methods of assessing the in vivo efficacy of a test compound against a particular cancer. In certain embodiments, the inventive methods are high-throughput methods. For example, hundreds or thousands of inventive compounds may be evaluated in parallel. In some embodiments, in order to identify chemical scaffolds with broad cytotoxicity toward retinoblastoma, libraries of compounds can be screened against two different cell lines in parallel.
In certain embodiments, the instant invention provides a method of screening a library of compounds based on a cytotoxicity assay. In some embodiments, the instant invention includes a method of identifying compounds which inhibit cellular proliferation. This method comprises the steps of first providing a test compound of interest, contacting the test compound with cells of interest, and incubating the test compound and cells under suitable conditions to determine if the compound inhibits the proliferation of the cells. The antiproliferative activity of the compounds of interest can be assessed using any of the methods known in the medical arts.
In certain embodiments, the library of compounds to be tested comprises novel compounds. In certain embodiments, the library of compounds comprises natural products and their derivatives; synthetic and natural toxic substances; inhibitors of DNA/RNA synthesis, protein synthesis, cellular respiration, and membrane integrity; and classical and experimental pesticides, herbicides, and endocrine disruptors. In some embodiments, the library of compounds comprises alkaloids, sesquiterpenes, diterpenes, pentacyclic triterpenes, and/or sterols. In certain embodiments, the library of compounds comprises compounds of one of the following formulae:
In some embodiments, the library of compounds comprises cardenolides and novel derivatives thereof. In some embodiments, the library of compounds comprises cardiac glycosides. In certain embodiments, the library of compounds comprises compounds with molecular structures related to a known cardenolide such as, for instance, digoxin or ouabain.
Any cells including normal and abnormal cells may be used in the inventive methods. Cells used in the inventive method may be ocular cancer cells. In some embodiments, the cells used in the inventive method are retinoblastoma cells. In certain embodiments, the cells are human retinoblastoma cells such as, for instance, Y79, WERI-Rb-1, and RB355 cells. In certain embodiments, the cells utilized in the inventive method are a luciferase-expressing Y79LUC cell line. In some embodiments, the cells utilized in the inventive method are human uveal melanoma C918 cells. In some embodiments, the cells utilized in the inventive method are human uveal melanoma Mum2b cells.
In certain embodiments, the cells are incubated with a test compound for approximately 1 minute to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 1 hour to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 12 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 24 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 36 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 120 hours. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 96 hours. In certain embodiments, the cells are incubated with a test compound for approximately 62 hours to approximately 82 hours. In certain embodiments, the cells are incubated with a test compound for approximately 72 hours. In certain embodiments, the cells are incubated with a test compound for 1, 2, 3, 4, 5, 6, or 7 days.
In some embodiments, the instant invention provides a method of determining dose response using a cytotoxicity assay. In some embodiments, the instant invention includes a method of performing dose response studies comprising the steps of providing a test compound, contacting the test compound with a cell, and incubating the cell with the compound under suitable conditions to determine the cytotoxicity of the compound. The antiproliferative activity of the test compound can then be assessed using a method known to those of ordinary skill in the art. This process can then be repeated using different concentrations of a test compound in order to calculate the IC50. In certain embodiments, the test compound is an inventive compound. In certain embodiments, the cells are retinoblastoma cells.
In certain embodiments, the cells are incubated with a test compound (e.g., an inventive compound) for approximately 1 minute to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 1 hour to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 12 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 24 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 36 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 1 week. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 120 hours. In certain embodiments, the cells are incubated with a test compound for approximately 48 hours to approximately 96 hours. In certain embodiments, the cells are incubated with a test compound for approximately 62 hours to approximately 82 hours. In certain embodiments, the cells are incubated with a test compound for approximately 72 hours. In certain embodiments, the cells are incubated with a test compound for approximately 1, 2, 3, 4, 5, 6, or 7 days.
In certain embodiments, after a specified amount of time (e.g., 72 hours) an indicator of cell viability (e.g., Alamar Blue) is added, and the mixture is incubated for an additional period of time. In some embodiments, this additional period of time ranges from approximately 1 hour to approximately 48 hours. In some embodiments, this additional period of time ranges from approximately 12 hour to approximately 36 hours. In some embodiments, this additional period of time is approximately 24 hours.
The inhibition of cell proliferation may be measured using methods or technology known in the art. In some embodiments, inhibition of cell proliferation is measured using a substance which produces a detectable signal that is proportional to the amount of inhibition of cell proliferation. In some embodiments, inhibition of cell proliferation is quantified using one of any indicators known to those of ordinary skill in the art that produces a quantifiable signal, the intensity of which is detectable and proportional to the amount of inhibition. In some embodiments, inhibition of cell proliferation is quantified using an indicator which fluoresces. Exemplary indicators include Tyramide-Alexa Fluor 488, Alamar Blue, etc.
As detailed herein, in assays to determine the ability of a compound (e.g., an inventive compound) to inhibit cancer cell growth certain compounds may exhibit IC50 values≦100 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦50 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦40 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦30 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦20 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦10 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦7.5 μM. In certain embodiments, the inventive compound exhibits IC50 values≦5 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦2.5 μM. In certain embodiments, the inventive compound exhibits IC50 values≦1 μM. In certain embodiments, the inventive compound exhibits IC50 values≦0.75 μM. In certain embodiments, the inventive compound exhibits IC50 values≦0.5 μM. In certain embodiments, the inventive compound exhibits IC50 values≦0.25 μM. In certain embodiments, the inventive compound exhibits IC50 values≦0.1 μM. In certain other embodiments, the inventive compound exhibits IC50 values≦75 nM. In certain other embodiments, the inventive compound exhibits IC50 values≦50 nM. In certain other embodiments, the inventive compound exhibits IC50 values≦25 nM. In certain other embodiments, the inventive compound exhibits IC50 values≦10 nM. In other embodiments, the inventive compound exhibits IC50 values≦7.5 nM. In other embodiments, the inventive compound exhibits IC50 values≦5 nM.
The instant invention also provides methods of inducing apoptosis comprising contacting a cell with an inventive compound in an effective amount to induce apoptosis. In some embodiments, the instant invention provides a method of inducing apoptosis comprising the steps of first providing an inventive compound, contacting the inventive compound with a cell, and then incubating the cell under suitable conditions to induce apoptosis. The extent of apoptosis can be assessed using methods known to detect cells undergoing apoptosis.
In certain embodiments, the cell is an ocular cancer cell. In certain embodiments, the cell is a retinoblastoma cell. In certain embodiments, the cell is derived from a human retinoblastoma cell line such as Y79, WERI-Rb-1, RB355, and Y79LUC. In certain embodiments, the cell is derived from a melanoma cell line such as a uveal melanoma cell line. In certain embodiments, the uveal melanoma cell line is C918 or Mum2b. In certain embodiments, the compound is of any one of the formulae described herein.
The method may further comprise administering a second chemotherapeutic agent, for instance, carboplatin, etoposide, teniposide, or verapamil to the cell. In some embodiments, the second chemotherapeutic agent is vincristine, calcitriol, melphalan, 5-fluorouracil, cyclosporin, cisplatin, or Nutlin-3. In certain embodiments, the second chemotherapeutic is an inhibitor of MDR1/Pgp. In some embodiments, the second chemotherapeutic agent is a multidrug resistance-associated protein-1 (MRP1) inhibitor. In some embodiments, the second chemotherapeutic agent is an ABC transporter inhibitor.
The extent of apoptosis may be measured using an indicator of apoptosis. In some embodiments, the extent of apoptosis is quantified using one of any indicators known to those of ordinary skill in the art that produces a quantifiable signal, the intensity of which is detectable and proportional to the extent of apoptosis. In some embodiments, the extent of apoptosis is determined using an indicator medium which fluoresces. In certain embodiments, the extent of apoptosis is determined using immunofluorescence detection of cleaved Caspase-3. In some embodiments, the extent of apoptosis is determined using nuclear staining techniques.
The instant invention also provides a method of assaying in vivo the efficacy of an inventive compound against tumor cells by providing a therapeutically effective amount of a compound in a composition suitable for administration to a host animal with a tumor, administering said composition to a host animal with a tumor, and lastly assessing the antitumor effect of the compound by monitoring the tumor over a period of time.
In some embodiments, the tumors are artificially implanted tumors. In some embodiments, the tumors are xenografts. In some embodiments, the tumors are xenografts comprising cells selected from any one of the group consisting of human retinoblastoma cells Y79, WERI-Rb-1, RB355, and Y79LUC. In some embodiments, the tumors are xenografts comprising Y79LUC cells embedded in matrigel. In some embodiments, the tumors are xenografts comprising cells selected from any one of the group consisting of human uveal melanoma cells lines C918 and Mum2b.
Xenograft tumors are typically grown in the host animal to a certain size prior to administration of an inventive compound. In some embodiments, xenografts are grown to a size ranging from approximately 50 to approximately 500 mm3. In some embodiments, xenografts are grown to a size ranging from approximately 100 to approximately 400 mm3. In some embodiments, xenografts are grown to a size ranging from approximately 200 to approximately 300 mm3. In some embodiments, xenografts are allowed to reach a size of at least approximately 250 mm3 prior to administration of the compound.
The method comprises administration of an inventive compound in a therapeutically effective dose to a host animal. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 0.1 mg/kg to approximately 50.0 mg/kg. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 0.5 mg/kg to approximately 50.0 mg/kg. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 0.5 mg/kg to approximately 40.0 mg/kg. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 0.5 mg/kg to approximately 30.0 mg/kg. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 1.0 mg/kg to approximately 25.0 mg/kg. In some embodiments, a therapeutically effective dose comprises an amount ranging from approximately 1.5 mg/kg to approximately 15.0 mg/kg. In some embodiments, treatment is administered locally. In some embodiments, treatment is administered by continuous infusion over a certain period of time. In certain embodiments, administration is via intraarterial infusion. In certain embodiments, administration is via intraarterial infusion via an artery feeding the tumor being treated. In some embodiments, when treatment of the eye is desired, intraarterial infusion occurs via the ophthalmic artery of the eye of the host animal.
In some embodiments, the efficacy of an inventive compound is measured by measuring tumor size over a period of time before, during, and/or after treatment with said compound. In some embodiments, tumor size is measured once a week. In some embodiments, tumor size is measured twice a week. In some embodiments, tumor size is measured daily. In some embodiments, tumor size is measured once a day. In some embodiments, tumor size is measured twice a day. In some embodiments, tumor size is measured once every other day. In some embodiments, tumor size is measured once every three days. In certain embodiments, tumor size is measured at intervals as required by any one of the methods known to those of skill in the art. In some embodiments, tumor size is measured externally twice a week with a caliper. In certain embodiments, tumor size is measured once a week using an imaging technique (e.g., MRI, X-ray, CT). In some embodiments, the imaging technique is bioluminescent imaging. In certain embodiments, bioluminescent imaging comprises anesthetization of the host animal, injection of a bioluminescent compound, and subsequent measurement of photonic emission. In some embodiments, imaging of the tumor is achieved using any of the methods known in the medical arts.
A subject may be any animal. In certain embodiments, the subject is any mammal (e.g., humans, domestic/veterinary/farm animals such as dogs, cats, cows, sheep, etc.). In some embodiments, the subject is a rodent. In certain embodiments, the subject is a human (e.g., child, juvenile, adult, male, female). In certain embodiments, the subject is an experimental animal such as a mouse, rat, dog, or non-human primate.
A therapeutically effective amount of a compound comprises administering an amount necessary to achieve a desired result. The exact amount required will vary from subject to subject, depending on the species, age, general condition of the subject, the severity of the disease, the particular anticancer agent, its mode of administration, the desired outcome, the xenograft, and the like.
In certain embodiments of the present invention, a “therapeutically effective amount” of a compound or pharmaceutical composition is that amount effective for inhibiting cell proliferation in a subject or a biological sample (e.g., in cells). In certain embodiments, cell proliferation is inhibited by about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, or about 99%. In certain embodiments, the compound inhibits cell proliferation by at least about 25%, at least about 50%, at least about 75%, or at least about 90%.
In certain embodiments of the present invention, a “therapeutically effective amount” refers to an amount of a compound or composition sufficient to inhibit cell proliferation, or refers to an amount of a compound or composition sufficient to reduce the tumor burden in a subject. In certain embodiments, the tumor burden is reduced by about 1%, about 5%, about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, or about 99%. In certain embodiments, the tumor burden is reduced by at least about 25%, at least about 50%, at least about 75%, or at least about 90%.
In certain embodiments of the present invention a “therapeutically effective amount” of the inventive compound or pharmaceutical composition is that amount effective for reducing or inhibiting the growth of tumor cells and/or killing tumor cells.
In another aspect, the present invention provides pharmaceutical compositions comprising an inventive compound, or a pharmaceutically acceptable form thereof, and a pharmaceutically acceptable excipient. In certain embodiments, a therapeutically effective amount of an inventive compound for the treatment of an ocular cancer is included in the pharmaceutical composition. In certain embodiments, a therapeutically effective amount of an inventive compound for the treatment of an ophthalmic condition is included in the pharmaceutical composition. In certain embodiments, a therapeutically effective amount of an inventive compound suitable for intraarterial administration is included in the pharmaceutical composition. In some embodiments, the cancer being treated is a hematopoietic cancer, a liposarcoma, a lung cancer, a brain cancer, a liver cancer, a pancreatic cancer, or an ocular cancer. In certain embodiments, the cancer is retinoblastoma, medulloepithelioma, ocular melanoma, or lymphoma.
It will also be appreciated that the compound of the instant invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable form thereof. According to the present invention, a pharmaceutically acceptable form includes, but is not limited to, pharmaceutically acceptable salts, esters, salts of such esters, or a prodrug or other adduct or derivative of a compound of the instant invention which upon administration to a subject in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite thereof.
As described above, the pharmaceutical compositions of the present invention comprise a pharmaceutically acceptable carrier, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants, and the like, as suited to the particular dosage form desired. Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutical compositions and known techniques for the preparation thereof. Except insofar as any conventional carrier medium is incompatible with a cardenolide, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutical composition, its use is contemplated to be within the scope of this invention. Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose, and cellulose acetate; powdered tragacanth; malt; gelatine; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil, sesame oil; olive oil; corn oil and soybean oil; glycols; such as propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogenfree water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator.
Injectable preparations, for example, sterile injectable aqueous or oleaginous may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland or fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
Compound of the instant invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage. The expression “dosage unit form” as used herein refers to a physically discrete unit of therapeutic agent appropriate for the subject to be treated. It will be understood, however, that the total daily usage of the compound and/or composition of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well known in the medical arts (see, for example, Goodman and Gilman's, The Pharmacological Basis of Therapeutics, Tenth Edition, A. Gilman, J. Hardman and L. Limbird, eds., McGraw-Hill Press, 155-173, 2001, which is incorporated herein by reference in its entirety).
Furthermore, after formulation with an appropriate pharmaceutically acceptable excipient in a desired dosage, the pharmaceutical compositions of this invention can be administered to humans and other animals
In certain embodiments, the compound of the invention may be administered at dosage levels of approximately 0.001 mg/kg to approximately 50 mg/kg, from approximately 0.01 mg/kg to approximately 25 mg/kg, or from approximately 0.1 mg/kg to approximately 10 mg/kg of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect. It will also be appreciated that dosages smaller than approximately 0.001 mg/kg or greater than approximately 50 mg/kg can be administered to a subject.
It will also be appreciated that the compound and/or pharmaceutical composition of the present invention can be formulated and employed in combination therapies, that is, the compound and/or pharmaceutical composition can be formulated with or administered concurrently with, prior to, or subsequent to, one or more other desired therapeutic agents. The particular combination of therapies (e.g., chemotherapy, radiation therapy, cryotherapy, brachytherapy, etc.) to be employed in a combination regimen will take into account compatibility of the desired therapeutics and/or therapies and the desired therapeutic effect to be achieved. It will also be appreciated that the therapies employed may achieve a desired effect for the same disorder (for example, an inventive compound may be administered concurrently with another anticancer agent, or they may achieve different effects (e.g., control of any adverse effects).
For example, other therapies or anticancer agents that may be used in combination with an inventive compound include surgery, radiotherapy (e.g., γ-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachytherapy, and systemic radioactive isotopes, etc.), endocrine therapy, biologic response modifiers (e.g., interferons, interleukins, and tumor necrosis factor (TNF)), hyperthermia and cryotherapy, agents to attenuate any adverse effects (e.g., antiemetics), and other approved chemotherapeutic drugs, including, but not limited to, alkylating drugs (mechlorethamine, chlorambucil, Cyclophosphamide, Melphalan, Ifosfamide), antimetabolites (Methotrexate), purine antagonists and pyrimidine antagonists (6-Mercaptopurine, 5-Fluorouracil, Cytarabile, Gemcitabine), spindle poisons (Vinblastine, Vincristine, Vinorelbine, Paclitaxel), podophyllotoxins (Etoposide, Irinotecan, Topotecan), antibiotics (Doxorubicin, Bleomycin, Mitomycin), nitrosoureas (Carmustine, Lomustine), inorganic ions (Cisplatin, Carboplatin), enzymes (Asparaginase), and hormones (Tamoxifen, Leuprolide, Flutamide, and Megestrol), to name a few. For a more comprehensive discussion of updated cancer therapies see, The Merck Manual, Seventeenth Ed. 1999, the entire contents of which are hereby incorporated by reference. See also the National Cancer Institute (CNI) website (www.nci.nih.gov) and the Food and Drug Administration (FDA) website for a list of the FDA approved oncology drugs (www.fda.gov/cder/cancer/druglistframe).
In certain embodiments, the pharmaceutical composition of the present invention further comprises one or more additional therapeutic agents (e.g., chemotherapeutic and/or palliative agents). For example, additional therapeutic agents for conjoint administration or inclusion in a pharmaceutical composition with an inventive compound may be an approved chemotherapeutic agent and/or palliative agent, or it may be any one of a number of agents undergoing approval by the Food and Drug Administration. For purposes of the invention, the term “palliative” refers to treatment that is focused on the relief of symptoms of a disease and/or side effects of a therapeutic regimen, but is not curative. For example, palliative treatment encompasses painkillers and antinausea medications. In addition, chemotherapy, radiotherapy, and surgery can all be used palliatively (that is, to reduce symptoms without going for cure; e.g., for shrinking tumors and reducing pressure, bleeding, pain, and/or other symptoms or signs of cancer).
The representative examples which follow are intended to help illustrate the invention, and are not intended to, nor should they be construed to, limit the scope of the invention. Indeed, various modifications of the invention and many further embodiments thereof, in addition to those shown and described herein, will become apparent to those skilled in the art from the full contents of this document, including the examples which follow and the references to the scientific and patent literature cited herein. It should further be appreciated that, unless otherwise indicated, the entire contents of each of the references cited herein are incorporated herein by reference to help illustrate the state of the art. The following examples contain important additional information, exemplification and guidance which can be adapted to the practice of this invention in its various embodiments and the equivalents thereof.
Cell Lines and Tissue Culture.
The human retinoblastoma cell lines Y79 and WERI-Rb-1 were purchased from the American Type Culture Collection (Manassas, Va.). The human retinoblastoma cell line RB355 originally established by Dr. Brenda Gallie (University of Toronto) and the luciferase-expressing Y79LUC cell line were kindly provided by Dr. Michael Dyer (Saint Jude Children's Research Hospital). The human uveal melanoma cell lines C918 and Mum2b originally established by Dr. Mary Hendrix (University of Iowa) were generously provided by Dr. Daniel Albert (University of Wisconsin). The cell lines Y79, WERI-Rb-1 and RB355 were grown in RPMI 1640 (Invitrogen, Carlsbad, Calif.) with 20% (v/v) fetal bovine serum (Omega Scientific, Tarzana, Calif.), 1% (v/v) penicillin-streptomycin (Gemini Bio-Products, Sacramento, Calif.), 2 mM glutamine (Invitrogen, Carlsbad, Calif.), 1 mM sodium pyruvate (Invitrogen, Carlsbad, Calif.), 4.5 g/L glucose (Invitrogen, Carlsbad, Calif.). The cell line C918 was cultured in DMEM with 10% (v/v) fetal bovine serum (Omega Scientific, Tarzana, Calif.), and 1% (v/v) penicillin-streptomycin. All cell lines were grown under an atmosphere of 5% CO2 95% air at 37° C. under 85% humidity.
Library compounds were pre-plated in 1 μL of 1% DMSO (v/v) into 1536-well microtiter plates (#3893, Corning Inc., Corning, N.Y.) using a TPS-384 Total Pipetting Solution (Apricot Designs. Monrovia, Calif.). Cells were added in 8 μl, medium to the screening plates using a Flexdrop IV (Perkin Elmer, Waltham, Mass.). After 72 h incubation was added 1 μL Alamar Blue using Flexdrop. The cells were then incubated for another 24 h, and the fluorescence intensity was read on the Amersham LEADseeker™ Multimodality Imaging System equipped with Cy3 excitation and excitation filters and FLINT epi-mirror. The signal inhibition induced by the compounds was expressed as a percentage compared to high and low controls located on the same plate, as defined as % Inhibition=(high control average−read value)/(high control average−low control average)×100.
Dose response studies were performed in 384-well microtiter plates (#3712, Corning Inc., Corning, N.Y.) according to the following protocol: cells were added in 45 μL medium to the screening plates using Flexdrop. After 72 h incubation was added 5 μL Alamar Blue using Flexdrop. The cells were then incubated for another 24 h, and the fluorescence intensity was read on LEADseeker™ Multimodality Imaging System as previously described. To calculate the IC50 for each compound toward each cell line, the dose response was assessed in duplicate and using 12 point doubling dilutions with 100 μM compound concentration as the upper limit. The dose response curve for each set of data was fitted separately, and the two IC50 values obtained were averaged. For compounds having an IC50 below 1 μM or 0.1 μM, the dose response study was repeated using dilutions starting at 10 μM or 1 μM for more accurate determination of the IC50 value.
Automation System & Screening Data Management.
The assays were performed on a fully automated linear track robotic platform (CRS F3 Robot System, Thermo Electron, Canada) using several integrated peripherals for plate handling, liquid dispensing, and fluorescence detection. Screening data files from the Amersham LEADseeker™ Multimodality Imaging System were loaded into the HTS Core Screening Data Management System, a custom built suite of modules for compound registration, plating, data management, and powered by ChemAxon Cheminformatic tools (ChemAxon, Hungary).
Chemical Libraries, Automation System & Screening Data Management.
The library used for the pilot screen combines 2,640 chemicals obtained commercially from Prestwick and MicroSource. The MicroSource Library contains 2,000 biologically active and structurally diverse compounds from known drugs, experimental bioactives, and pure natural products. The library includes a reference collection of 160 synthetic and natural toxic substances (inhibitors of DNA/RNA synthesis, protein synthesis, cellular respiration, and membrane integrity), a collection of 80 compounds representing classical and experimental, pesticides, herbicides, and endocrine disruptors, a unique collection of 720 natural products and their derivatives. The collection includes simple and complex oxygen heterocycles, alkaloids, sesquiterpenes, diterpenes, pentercyclic triterpenes, sterols, and many other diverse representatives. The Prestwick Chemical Library is a unique collection of 640 high purity chemical compounds, all off patent and carefully selected for structural diversity and broad spectrum, covering several therapeutic areas from neuropsychiatry to cardiology, immunology, anti-inflammatory, analgesia and more, with known safety, and bioavailability in humans. The library is constituted of 90% of marketed drugs and 10% bioactive alkaloids or related substances.
Y79 cells seeded in culture medium in a 24-well plate were treated with either vincristine, etoposide, or ouabain at various concentrations in 1% DMSO (v/v) or with 1% DMSO (v/v) alone as a carrier control for 48 h or 72 h. After a wash in PBS, cells were fixed in solution in 4% (v/v) in PBS for 10 minutes. After a wash in PBS, cells for each condition were dried on a glass slide and washed once with water.
The immunofluorescence detection of cleaved Caspase-3 was performed at the Memorial Sloan-Kettering Cancer Center Molecular Cytology Core Facility using a Discovery XT processor (Ventana Medical Systems, Tucson, Ariz.). A rabbit polyclonal Cleaved Caspase 3 (Asp175) antibody (#9661L, Cell Signaling, Danvers, Mass.) was used at a concentration of 0.1 μg/ml. Cells were blocked for 30 minutes in 10% (v/v) normal goat serum, 2% (v/v) BSA in PBS prior to incubation with the primary antibody for 3 hours and subsequent 20 minutes incubation with biotinylated goat anti-rabbit IgG (#PK6101, Vector labs, Burlingame, Calif.) diluted 1:200. The detection was performed with Secondary Antibody Blocker, Blocker D, Streptavidin-HRP D (Ventana Medical Systems, Tucson, Ariz.), followed by incubation with Tyramide-Alexa Fluor 488 (#T20922, Invitrogen, Carlsbad, Calif.). Nuclear staining was then performed by incubating the slides for 15 minutes in a 8 μM Hoechst 33342 (Molecular Probes, Eugene, Oreg.) solution in PBS and washing once with PBS. Automated fluorescence imaging of the green channel (activated Caspase-3) and blue channel (nuclei) was performed using an IN Cell Analyzer 1000 (GE Healthcare).
Subcutaneous xenograft experiments were performed at the Memorial Sloan-Kettering Cancer Center Antitumor Assessment Core Facility. Y79LUC cells (10E6) embedded in matrigel (BD Biosciences, San Jose, Calif.) were injected subcutaneously in the right flank of 8-weeks old ICR/SCID male mice. Treatment started when the tumors reached approximately 250 mm3. The mice were randomized into three groups and two mice per group were treated with either 10% DMSO (v/v) (control group), 1.5 mg/kg ouabain or 15 mg/kg ouabain. Treatment was performed by continuous subcutaneous infusion using osmotic minipumps (#1007D, Alzet, Cupertino, Calif.). Tumor size was measured externally two times a week with a caliper. Body weight of each mouse was monitored as well as other signs of toxicity throughout the treatment period. Bioluminescent imaging of the tumors was performed once a week and prior to sacrifice as follows: mice were anesthetized by isofluorane inhalation and injected with D-luciferin at 50 mg/Kg (Xenogen) intraperitoneally; photonic emission was measured with the In Vivo Imaging System (IVIS 200, Xenogen) with a collection time of 5 seconds.
The aim was to identify alternative cytotoxic agents for retinoblastoma among known drugs and bioactive agents. To meet this goal, a combined library of 2,640 commercially-obtained chemicals representing biologically active and structurally diverse compounds from known drugs, experimental bioactives, and pure natural products, were screened, most of which were off-patent. For the screen, the use of the well-described cytotoxicity assay based on the reduction of the dye resazurin and commercially sold as Alamar Blue 28 (Ahmed et al., J. Immunol. Methods 1994, 170, 211-224) was relied on due to its compatibility with the requirements of high-throughput screening (Shum et al., J. Enzyme Inhib. Med. Chem. 2008, 1) In this assay, the fluorescence emitted by the living cells upon metabolism of Alamar Blue is proportional to the number of metabolically active cells. Hence, the cytotoxicity or the cytostaticity of a compound can be assessed relative to a control. In an effort to identify chemical scaffolds with broad activity for retinoblastoma as opposed to compounds only cytotoxic toward one specific retinoblastoma cell line, a strategy was adopted to screen the combined drug library in parallel against two retinoblastoma cell lines. Y79 (Reid et al., J. Natl. Cancer Inst. 1974, 53, 347-360) and the RB355 (Fournier et al., Invest. Ophthalmol. Vis. Sci. 1987, 28, 690-699) human cell lines were chosen as models of retinoblastoma because they are among the few well-established human retinoblastoma cell lines available and because it was possible to optimize their growth in high density format. Duplicate sets of the combined library of 2,640 compounds were tested at 10 μM consecutively the same day for each cell line. After statistical analysis of the duplicate sets of data to assess the reproducibility of the screen and to ensure the absence of systematic error, it was calculated the average percentage inhibition for each compound based on high and low controls present on each plate as previously described (Antczak et al., J. Biomol. Screen 2007, 12, 521-535). When the newly generated Y79 and RB355 data sets were compared, it was found that a large population of the tested compounds was active only toward one of the two cell lines (
Cytotoxicity profiling was performed for these 11 positives against the human retinoblastoma cell lines Y79, RB355 and WERI-Rb-1, as well as against the uveal melanoma cell lines C918 and Mum2b. It was found that all 11 selected positives had broad and potent cytotoxic activity against these five ocular cancer cell lines with calculated IC50s ranging from 40 nM to 27 μM (
Cardenolides Constitute a Class of Drugs with Broad and Potent Cytotoxic Activity Toward Ocular Cancer Cells.
A structural analysis of the positives identified during the screen revealed that the five ion pump effectors that we previously characterized (
To explore the structure activity relationship (SAR) within this chemical class a collection of 35 cardenolides and derivatives was constituted. The dose response for each compound toward the ocular cancer cell lines Y79, RB355, WERI-Rb-1 and C918 was then assessed. The results of this structure-activity relationship (SAR) study are summarized in
The potency of a representative of the cardenolide scaffold was compared to known effective agents against retinoblastoma. Namely, the dose response of the drug ouabain was tested with the human retinoblastoma cell lines Y79 and RB355, and its potency was compared to vincristine, etoposide, carboplatin, cisplatin, nutlin-3 and calcitriol. Ouabain was chosen as a representative of cardenolides because it demonstrated broad and potent activity toward all the tested cell lines (
To determine whether the anti-proliferative effects of the drug ouabain was mediated by induction of apoptosis, immunostaining of activated Caspase-3 in Y79 cells treated with cardenolides or known agents for retinoblastoma for 72 h (green channel) was performed; treated cells were also stained with Hoechst to image the nuclei (blue channel) (
The therapeutic effect of the drug ouabain in a mouse xenograft model of retinoblastoma was also investigated. Three groups of two 8 weeks old ICR/SCID male mice bearing Y79 tumors implanted in the flank were treated with either vehicle only, 1.5 mg/kg ouabain or 15 mg/kg ouabain. Mice were continuously infused subcutaneously using an osmotic minipump delivery system. Evaluation of tumor burden by bioluminescent imaging shows that ouabain at 15 mg/kg rapidly induced a dramatic decrease in tumor size leading to complete tumor regression (as assessed by bioluminescence imaging) after 14 days of treatment (
This research presents an alternative strategy aiming at identifying novel agents for treating retinoblastoma among already approved drugs. Known drugs may have previously unreported antiproliferative properties for retinoblastoma, and could therefore potentially be repositioned as novel drugs for retinoblastoma cells. To investigate this, a combined library of 2,640 marketed drugs and bioactive compounds was compiled and a cytotoxicity assay amenable to high-throughput screening for the human retinoblastoma cell lines Y79 and RB355 was developed. A striking finding of this screening campaign was the discovery of the broad and potent antiproliferative activity toward retinoblastoma cells of the well-described chemical class of cardenolides. This observation was confirmed by establishing basic structure activity relationships (SAR) for a series of 35 cardenolides and derivatives in a panel of four ocular cancer cell lines. When the in vitro antiproliferative properties of the drug ouabain was compared to known or experimental agents for retinoblastoma, it was observed that the potency of ouabain is comparable to agents currently used in clinic. Furthermore, it was demonstrated that the drug ouabain induces apoptosis in Y79 human retinoblastoma cells at a dose of 0.5 μM. This observation is in agreement with previous studies showing that cardenolides induce apoptosis in various cell types (Newman et al., Mol. Interv. 2008, 8, 36-49. Finally, when the therapeutic effect of ouabain was assessed in a xenograft model of retinoblastoma, a drastic response leading to complete tumor regression after 14 days of treatment was observed. Even at the high dose of 15 mg/kg ouabain used in this study, no signs of toxicity were observed.
A collection of 45 cardenolides and derivatives was constituted. The cytotoxicity profiling of those 45 compounds was performed on a panel of human ocular cancer cell lines:
Dose response studies were performed in 384-well microtiter plates (#3712, Corning Inc., Corning, N.Y.) according to the following protocol: cells were added in 45 μL medium to the screening plates using Flexdrop. After 72 h incubation was added 5 μL Alamar Blue using Flexdrop. The cells were then incubated for another 24 h, and the fluorescence intensity was read on LEADseeker™ Multimodality Imaging System as previously described. To calculate the IC50 for each compound toward each cell line, the dose response was assessed in duplicate and using 12 point doubling dilutions with 100 μM compound concentration as the upper limit. The dose response curve for each set of data was fitted separately, and the two IC50 values obtained were averaged. For compounds having an IC50 below 1 μM, the dose response study was repeated using dilutions starting at 10 μM or 1 μM for more accurate determination of the IC50 value. IC50 of 0.1 corresponds to <0.1 μM and IC50 of 100 corresponds to >100 μM.
A 4-year old boy with stage Vb retinoblastoma (international classification group E) of the right eye presented after incomplete response to three cycles of intraarterial melphalan chemotherapy administered elsewhere 7 weeks, 4 weeks, and 3 days prior to presentation. Fundus examination revealed a solitary, cream-colored, 12×6 mm tumor with type III regression, a retinal detachment, and extensive, non-calcified vitreous seeds (
Three weeks later, there was no regression on the main tumor or vitreous seeds, and after consideration of the treatment options, the family opted to try intraarterial digoxin. Institutional review board approvals were obtained, and an Investigational New Drug (IND) permit was provided by the FDA.
Exam remained stable three weeks after intraarterial digoxin (25 μg) into the ophthalmic artery as previously described for melphalan (Abramson et al., Ophthalmology 2008; 115:1398-1404). Three weeks after a second round of intraarterial digoxin at a higher dose (125 μg), there was decreased tumor size, fewer vitreous seeds, and resolution of the retinal detachment (
The patient was treated with oral digoxin (10 μg/kg daily) for three weeks in his home country. He suffered no side effects or EKG abnormalities, and the serum digoxin level remained in the cardiac target range. After three weeks, reexamination revealed progression of disease (
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/US2010/058567 | 12/1/2010 | WO | 00 | 10/10/2012 |
| Number | Date | Country | |
|---|---|---|---|
| 61265961 | Dec 2009 | US | |
| 61308059 | Feb 2010 | US |
