Patent Grant
8304406
This application is a National Stage Application filed under 35 U.S.C. §371 based on International Application No. PCT/EP2007/002985, filed Apr. 3, 2007, which claims priority to European Patent Application Number 06007929.0, filed Apr. 15, 2006, the entire contents each of which are incorporated herein by reference.
The foregoing applications, and all documents cited therein and all documents cited or referenced therein, and all documents cited or referenced herein, including any U.S. or foreign patents or published patent applications, International patent applications, as well as, any non-patent literature references and any manufacturer's instructions, are hereby expressly incorporated herein by reference.
The present invention relates to pharmaceutical compositions and combinations for treating, preventing or managing pulmonary hypertension comprising small molecule heterocyclic pharmaceuticals, and more particularly, substituted pyridines and pyridazines optionally combined with at least one additional therapeutic agent.
Substituted pyridines and pyridazines as mentioned in the present invention are described in WO 01/023375 for treating abnormal angiogenesis or hyperpermeability processes which are linked to cancer.
Pulmonary hypertension refers to a disease characterized by sustained elevations of pulmonary artery pressure (L. J. Rubin, The New England Journal of Medicine, 1997, 336(2), 111). Current treatment of pulmonary hypertension depends on the stage and the mechanism of the disease. Typical treatments for pulmonary hypertension include anticoagulation, oxygen supplementation, conventional vasodilator therapy, transplantation and surgical care. Therapeutic agents presently used for the treatment of pulmonary hypertension include e.g. calcium channel blockers and pulmonary vasodilators
The present invention provides pharmaceutical compositions for treating, preventing or managing pulmonary hypertension comprising a compound of formula I and optionally at least one further therapeutic agent.
The present invention can be used e.g. by administering a compound of formula I and optionally a further therapeutic agent, pharmaceutically-acceptable salts thereof, and derivatives thereof, etc.
The compounds with the structure of formula I, pharmaceutically acceptable salts, polymorphs, solvates, hydrates metabolites and prodrugs thereof, including diastereoisomeric forms (both isolated stereoisomers and mixtures of stereoisomers) are collectively referred to herein as the “compounds of formula I”.
In its broadest aspect, the present invention relates to the sum of three sets of chemical compounds, or pharmaceutically acceptable salts or prodrugs thereof, with each set overlapping the others in scope. The generalized structural formula for the compounds in each of the three sets of compounds is the same, but it should be noted that the definitions of the several groups comprising the general structure in each set differ somewhat. Thus, the defined sets of chemical compounds differ from each other, but overlap in their scopes.
The first set of compounds have the generalized structural formula
wherein
In the above substructures, G1 is a substituent independently selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted saturated heterocyclylalkyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted partially unsaturated heterocyclylalkyl; —OCO2R3; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —CHO; —OCON(R6)2; —NR3CO2R6; and —NR3CON(R6)2
The group R3 is H or lower alkyl. R6 is independently selected from the group consisting of H; alkyl; cycloalkyl; optionally substituted aryl; optionally substituted aryl lower alkyl, lower alkyl-N(R3)2, and lower alkyl-OH.
In generalized structural formula (I), R4 is H, halogen, or lower alkyl. The subscript p is 0, 1, or 2; and X is selected from the group consisting of O, S, and NR3.
The linking moiety Y is selected from the group consisting of lower alkylene; —CH2—O—; —CH2—S—; —CH2—NH—; —O—; —S—; —NH—; —O—CH2—; —S(O)—; —S(O)2—; —SCH2—; —S(O)CH2—; —S(O)2CH2—; —CH2S(O)—; —CH2S(O)2; —(CR42)n—S(O)p-(5-membered heteroaryl)-(CR42)s—; and —(CR42)n—C(G2)(R4)—(CR42)n—. In the latter two linking groups Y, n and s are each independently 0 or an integer of 1-2. The substituent G2 is selected from the group consisting of —CN, —CO2R3, —CON(R6)2, and —CH2N(R6)2.
Z represents CR4 or N.
Regarding the ring containing A, B, D, E, and L, the number of possible substituents G3 on the ring is indicated by subscript q, which is 0, 1, or 2.
Substituent moieties G3 are monovalent or bivalent moieties selected from the group consisting of: lower alkyl; —NR3COR6; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; —OR6; —SR6; —S(O)R6; —S(O)2R6; —OCOR6; —COR6; —CO2R6; —CH2OR3; —CON(R6)2; —S(O)2N(R6)2; —NO2; —CN; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and bivalent bridge of structure T2=T2−T3. In this bivalent bridge, each T2 independently represents N, CH, or CG3′; and T3 represents S, O, CR4G3′, C(R4)2, or NR3. G3′ represents any of the above-defined moieties G3 which are monovalent; and the terminal T2 of the bridge is bound to L, and T3 is bound to D, thus forming a 5-membered fused ring.
In the ring shown at the left in generalized structural formula (I), A and D independently represent N or CH; B and E independently represent N or CH; and L represents N or CH; with the provisos that a) the total number of N atoms in the ring containing A, B, D, E, and L is 0, 1, 2, or 3; b) when L represents CH and any G3 is a monovalent substituent, at least one of A and D is an N atom; and c) when L represents CH and a G3 is a bivalent bridge of structure T2=T2−T3, then A, B, D, and E are also CH.
J is a ring selected from the group consisting of aryl; pyridyl; and cycloalkyl. The subscript q′ represents the number of substituents G4 on ring J and is 0, 1, 2, 3, 4, or 5.
The possible substituents G4 on ring J are monovalent or bivalent moieties selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; —OCO2R3; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted hetero-arylalkyl); —CHO; —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and fused ring-forming bivalent bridges attached to and connecting adjacent positions of ring J, said bridges having the structures:
When G4 is an alkyl group located on ring J adjacent to the linkage —(CR42)p—, and X is NR3 wherein R3 is an alkyl substituent, then G4 and the alkyl substituent R3 on X may be joined to form a bridge of structure —(CH2)p′— wherein p′ is 2, 3, or 4, with the proviso that the sum of p and p′ is 2, 3, or 4, resulting in formation of a nitrogen-containing ring of 5, 6, or 7 members.
Additional provisos are that: 1) in G1, G2, G3, and G4, when two groups R3 or R6 are each alkyl and located on the same N atom they may be linked by a bond, an O, an S, or NR3 to form a N-containing heterocycle of 5-7 ring atoms; and 2) when an aryl, heteroaryl, or heterocyclyl ring is optionally substituted, that ring may bear up to 5 substituents which are independently selected from the group consisting of amino, mono-loweralkyl-substituted amino, di-loweralkyl-substituted amino, lower alkanoylamino, halogeno, lower alkyl, halogenated lower alkyl, hydroxy, lower alkoxy, lower alkylthio, halogenated lower alkoxy, halogenated lower alkylthio, lower alkanoyl-oxy, —CO2R3, —CHO, —CH2OR3, —OCO2R3, —CON(R6)2, —OCON(R6)2, —NR3CON(R6)2, nitro, amidino, guanidino, mercapto, sulfo, and cyano; and 3) when any alkyl group is attached to O, S, or N, and bears a hydroxyl substituent, then said hydroxyl substituent is separated by at least two carbon atoms from the O, S, or N to which the alkyl group is attached.
The second set of compounds have the generalized structural formula
In the above bridge substructures, the subscript m is 0 or an integer 1-4; indicating that the resultant fused rings may optionally bear up to four substituents G1.
G1 is a substituent independently selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted saturated heterocyclylalkyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted partially unsaturated heterocyclylalkyl; —OCO2R3; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —CHO; —OCON(R6)2; —NR3CO2R6; and —NR3CON(R6)2
The group R3 is H or lower alkyl. R6 is independently selected from the group consisting of H; alkyl; cycloalkyl; optionally substituted aryl; optionally substituted aryl lower alkyl; lower alkyl-N(R3)2, and lower alkyl-OH.
In generalized structural formula (I), R4 is H, halogen, or lower alkyl; the subscript p is 0, 1, or 2; and X is selected from the group consisting of O, S, and NR3.
The linking moiety Y is selected from the group consisting of lower alkylene; —CH2—O—; —CH2—S—; —CH2—NH—; —O—; —S—; —NH—; —O—CH2—; —S(O)—; —S(O)2—; —SCH2—; —S(O)CH2—; —S(O)2CH2—; —CH2S(O)—; —CH2S(O)2—; —(CR42)n—S(O)p-(5-membered heteroaryl)-(CR42)s—; and —(CR42)n—C(G2)(R4)—(CR42)n—. In the latter two linking groups Y, subscripts n and s are each independently 0 or an integer of 1-2. G2 is selected from the group consisting of —CN, —CO2R3, —CON(R6)2, and —CH2N(R6)2.
Z represents N or CR4.
Regarding the ring containing A, B, D, E, and L, the number of possible substituents G3 on the ring is indicated by the subscript q, which is 1 or 2.
Substituents G3 are monovalent or bivalent moieties selected from the group consisting of lower alkyl; —NR3COR6; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; —OR6; —SR6; —S(O)R6; —S(O)2R6; —OCOR6; —COR6; —CO2R6; —CH2OR3; —CON(R6)2; —S(O)2N(R6)2; —NO2; —CN; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and bivalent bridge of structure T2=T2−T3. In this bivalent bridge, each T2 independently represents N, CH, or CG3′; and T3 represents S, O, CR4G3′, C(R4)2, or NR3. G3′ represents any of the above-defined moieties G3 which are monovalent; and the terminal T2 is bound to L, and T3 is bound to D, thus forming a 5-membered fused ring.
In the ring shown at the left in generalized structural formula (I), A and D independently represent CH; B and E independently represent CH; and L is CH; with the proviso that the resulting phenyl ring bears as a G3 substituent said bivalent bridge of structure T2=T2−T3.
J is a ring selected from the group consisting of aryl; pyridyl; and cycloalkyl. The subscript q′ represents the number of substituents G4 on ring J and is 0, 1, 2, 3, 4, or 5.
G4 is a monovalent or bivalent moiety selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkyl-amino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoyl-amino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; —OCO2R3; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —CHO; —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and fused ring-forming bivalent bridges attached to and connecting adjacent positions of ring J, said bridges having the structures:
When G4 is an alkyl group located on ring J adjacent to the linkage —(CR42)p—, and X is NR3 wherein R3 is an alkyl substituent, then G4 and the alkyl substituent R3 on X may be joined to form a bridge of structure —(CH2)p′— wherein p′ is 2, 3, or 4, with the proviso that the sum of p and p′ is 2, 3, or 4, resulting in formation of a nitrogen-containing ring of 5, 6, or 7 members.
Additional provisos are that: 1) in G1, G2, G3, and G4, when two groups R3 or R6 are each alkyl and located on the same. N atom they may be linked by a bond, an O, an S, or NR3 to form a N-containing heterocycle of 5-7 ring atoms; and 2) when an aryl, heteroaryl, or heterocyclyl ring is optionally substituted, that ring may bear up to 5 substituents which are independently selected from the group consisting of amino, mono-loweralkyl-substituted amino, di-loweralkyl-substituted amino, lower alkanoylamino, halogeno, lower alkyl, halogenated lower alkyl, hydroxy, lower alkoxy, lower alkylthio, halogenated lower alkoxy, halogenated lower alkylthio, lower alkanoyloxy, —CO2R3, —CHO, —CH2OR3, —OCO2R3, —CON(R6)2, —OCON(R6)2, —NR3CON(R6)2, nitro, amidino, guanidino, mercapto, sulfo, and cyano; and 3) when any alkyl group is attached to O, S, or N, and bears a hydroxyl substituent, then said hydroxyl substituent is separated by at least two carbon atoms from the O, S, or N to which the alkyl group is attached.
The third set of compounds have the generalized structural formula
In the above bridge structures, the subscript m is 0 or an integer 1-4; indicating that the resultant fused rings may optionally bear up to four substituents G1.
G1 is a substituent independently selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted saturated heterocyclylalkyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted partially unsaturated heterocyclylalkyl; —OCO2R3; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroaryl-alkyl); —CHO; —OCON(R6)2; —NR3CO2R6; and —NR3CON(R6)2.
The group R3 is H or lower alkyl. R6 is independently selected from the group consisting of H; alkyl; cycloalkyl; optionally substituted aryl; optionally substituted aryl lower alkyl; lower alkyl-N(R3)2, and lower alkyl-OH.
In generalized structural formula (I), R4 is H, halogen, or lower alkyl; the subscript p is 0, 1, or 2; and X is selected from the group consisting of O, S, and NR3.
The linking moiety Y is selected from the group consisting of lower alkylene; —CH2—O—; —CH2—S—; —CH2—NH—; —O—; —S—; —NH—; —O—CH2—; —S(O)—; —S(O)2—; —SCH2—; —S(O)CH2—; —S(O)2CH2—; —CH2S(O)—; —CH2S(O)2—; —(CR42)n—S(O)p-(5-membered heteroaryl)-(CR42)s—; and —(CR42)n—C(G2)(R4)—(CR42)s—. In the latter two linking groups Y, subscripts n and s are each independently 0 or an integer of 1-2. G2 is selected from the group consisting of —CN, —CO2R3, —CON(R6)2, and —CH2N(R6)2
Z represents CR4.
Regarding the ring containing A, B, D, E, and L, the number of possible substituents G3 on the ring is indicated by the subscript q, which is 1 or 2.
Substituents G3 are monovalent or bivalent moieties selected from the group consisting of —NR3COR6; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; —OR6; —SR6; —S(O)R6; —S(O)2R6; —OCOR6; —COR6; —CO2R6; —CH2OR3; —CON(R6)2; —S(O)2N(R6)2; —NO2; —CN; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and bivalent bridge of structure T2=T2−T3. In this bivalent bridge, each T2 independently represents N, CH, or CG3′; and T3 represents S, O, CR4G3′, C(R4)2, or NR3. G3′ represents any of the above-defined moieties G3 which are monovalent; and the terminal T2 is bound to L, and T3 is bound to D, thus forming a 5-membered fused ring.
In the ring shown at the left in generalized structural formula (I), A and D independently represent N or CH; B and E independently represent N or CH; and L represents N or CH; with the provisos that a) the total number of N atoms in the ring containing A, B, D, E, and L is 0, 1, 2, or 3; and b) when L represents CH and any G3 is a monovalent substituent, at least one of A and D is an N atom; and c) when L represents CH and a G3 is a bivalent bridge of structure T2=T2−T3, then A, B, D, and E are also CH.
J is a ring selected from the group consisting of aryl; pyridyl; and cycloalkyl. The subscript q′ represents the number of substituents G4 on ring J and is 0, 1, 2, 3, 4, or 5.
G4 is a monovalent or bivalent moiety selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; cycloalkyl; lower alkenyl; lower cycloalkenyl; halogen-substituted alkyl; amino-substituted alkyl; N-lower alkylamino-substituted alkyl; N,N-di-lower alkylamino-substituted alkyl; N-lower alkanoylamino-substituted alkyl; hydroxy-substituted alkyl; cyano-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; phenyl lower alkoxycarbonyl-substituted alkyl; halogen-substituted alkylamino; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; cyano-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; amidino; guanidino; sulfo; —B(OH)2; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted saturated heterocyclyl; optionally substituted partially unsaturated heterocyclyl; —OCO2R3; optionally substituted hetero-arylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); —CHO; —OCON(R6)2; —NR3CO2R6; —NR3CON(R6)2; and fused ring-forming bivalent bridges attached to and connecting adjacent positions of ring J, said bridges having the structures:
When G4 is an alkyl group located on ring J adjacent to the linkage —(CR42)p—, and X is NR3 wherein R3 is an alkyl substituent, then G4 and the alkyl substituent R3 on X may be joined to form a bridge of structure —(CH2)p′— wherein p′ is 2, 3, or 4, with the proviso that the sum of p and p′ is 2, 3, or 4, resulting in formation of a nitrogen-containing ring of 5, 6, or 7 members.
Additional provisos are that: 1) in G1, G2, G3, and G4, when two groups R3 or R6 are each alkyl and located on the same N atom they may be linked by a bond, an O, an S, or NR3 to form a N-containing heterocycle of 5-7 ring atoms; and 2) when an aryl, heteroaryl, or heterocyclyl ring is optionally substituted, that ring may bear up to 5 substituents which are independently selected from the group consisting of amino, mono-loweralkyl-substituted amino, di-loweralkyl-substituted amino, lower alkanoylamino, halogeno, lower alkyl, halogenated lower alkyl, hydroxy, lower alkoxy, lower alkylthio, halogenated lower alkoxy, halogenated lower alkylthio, lower alkanoyloxy, —CO2R3, —CHO, —CH2OR3, —OCO2R3, —CON(R6)2, —OCON(R6)2, —NR3CON(R6)2, nitro, amidino, guanidino, mercapto, sulfo, and cyano; and 3) when any alkyl group is attached to O, S, or N, and bears a hydroxyl substituent, then said hydroxyl substituent is separated by at least two carbon atoms from the O, S, or N to which the alkyl group is attached.
Pharmaceutically acceptable salts of these compounds as well as commonly used prodrugs of these compounds such as O-acyl derivatives of invention compounds which contain hydroxy groups are also within the scope of the invention.
The invention also relates to pharmaceutical compositions comprising one or more of the compounds of the invention, or their salts or prodrugs, in a pharmaceutically acceptable carrier.
The prefix “lower” denotes a radical having up to and including a maximum of 7 atoms, especially up to and including a maximum of 5 carbon atoms, the radicals in question being either linear or branched with single or multiple branching.
“Alkyl” means a hydrocarbon radical having up to a maximum of 12 carbon atoms, which may be linear or branched with single or multiple branching. Alkyl is especially lower alkyl.
Where the plural form is used for compounds, salts, and the like, this is taken to mean also a single compound, salt, or the like.
Any asymmetric carbon atoms may be present in the (R)-, (S)- or (R,S) configuration, preferably in the (R)- or (S)-configuration. Substituents at a double bond or a ring may be present in cis-(═Z—) or trans (=E-) form. The compounds may thus be present as mixtures of isomers or as pure isomers, preferably as enantiomer-pure diastereomers and having pure cis- or trans-double bonds.
Lower alkylene Y may be branched or linear but is preferably linear, especially methylene (—CH2), ethylene (—CH2—CH2), trimethylene (—CH2—CH2—CH2) or tetramethylene (—CH2CH2CH2CH2). When Y is lower alkylene, it is most preferably methylene.
“Aryl” means an aromatic radical having 6 to 14 carbon atoms, such as phenyl, naphthyl, fluorenyl or phenanthrenyl.
“Halogen” means fluorine, chlorine, bromine, or iodine but is especially fluorine, chlorine, or bromine.
“Pyridyl” means 1-, 2-, or 3-pyridyl but is especially 2- or 3-pyridyl.
“Cycloalkyl” is a saturated carbocycle that contains between 3 and 12 carbons but preferably 3 to 8 carbons.
“Cycloalkenyl” means a non-reactive and non-aromatic unsaturated carbocycle that contains between 3 and 12 carbons but preferably 3 to 8 carbons and up to three double bonds. It is well known to those skilled in the art that cycloalkenyl groups that differ from aromatics by lacking only one double bond such as cyclohaxadiene are not sufficiently non-reactive to be reasonable drug substances and therefor their use as substituents is not within the scope of this invention.
Cycloalkyl and cycloalkenyl groups may contain branch points such that they are substituted by alkyl or alkenyl groups. Examples of such branched cyclic groups are 3,4-dimethylcyclopentyl, 4-allylcyclohexyl or 3-ethylcyclopent-3-enyl.
Salts are especially the pharmaceutically acceptable salts of compounds of formula I such as, for example, acid addition salts, preferably with organic or inorganic acids, from compounds of formula I with a basic nitrogen atom. Suitable inorganic acids are, for example, halogen acids such as hydrochloric acid, sulfuric acid, or phosphoric acid. Suitable organic acids are, for example, carboxylic, phosphonic, sulfonic, or sulfamic acids, for example acetic acid, propionic acid, octanoic acid, decanoic acid, dodecanoic acid, glycolic acid, lactic acid, -hydroxybutyric acid, gluconic acid, glucosemonocarboxylic acid, fumaric acid, succinic acid, adipic acid, pimelic acid, suberic acid, azeiaic acid, malic acid, tartaric acid, citric acid, glucaric acid, galactaric acid, amino acids, such as glutamic acid, aspartic acid, N-methylglycine, acetylaminoacetic acid, N-acetylasparagine or N-acetylcysteine, pyruvic acid, acetoacetic acid, phosphoserine, 2- or 3-glycerophosphoric acid.
In the definition of Y, the diradical “-(5 member heteroaryl)-” denotes a 5-membered aromatic heterocycle containing 1-3 heteroatoms selected from O, S, and N, the number of N atoms being 0-3 and the number of O and S atoms each being 0-1 and connected to the sulfur from a carbon and to —(CR42)s— through a C or N atom. Examples of such diradicals include
In the definitions of G1, G2, G3, and G4 the statement is made that when two groups R3 or R6 are found on a single N, they can be combined into a heterocycle of 5-7 atoms. Examples of such heterocycles, including the N to which they are attached, are:
“Heterocyclyl” or “heterocycle” means a five- to seven-membered heterocyclic system with 1-3 heteroatoms selected from the group nitrogen, oxygen, and sulfur, which may be unsaturated or wholly or partly saturated, and is unsubstituted or substituted especially by lower alkyl, such as methyl, ethyl, 1-propyl, 2-propyl, or tert-butyl.
When an aryl, heteroaryl, or heterocyclyl ring is said to be optionally substituted, that ring may bear up to 5 substituents which are independently selected from the group consisting of amino, mono- or di-loweralkyl-substituted amino, lower alkanoylamino, halogeno, lower alkyl, halo-genated lower alkyl such as trifluoromethyl, hydroxy, lower alkoxy, lower alkylthio, halogenated lower alkoxy such as trifluoromethoxy, halogenated lower alkylthio such as trifluoromethylthio, lower alkanoyloxy, —CO2R3, —CHO, —CH2OR3, —OCO2R3, —CON(R6)2, —OCO N(R6)2, —NR3CON(R6)2, nitro, amidino, guanidino, mercapto, sulfo, and cyano.
In the ring attached to Y, the ring members A, B, D, E, and L may be N or CH, it being understood that the optional substituents G3 are necessarily attached to carbon and not nitrogen, and that when a given carbon bears a substituent group G3, that G3 group is in place of the H atom the carbon would bear in the absence of the G3 group.
Examples of ring J together with two adjacent G4 moieties which taken together form a second fused ring are:
“Heteroaryl” means a monocyclic or fused bicyclic aromatic system with between 5 and 10 atoms in total of which 1-4 are heteroatoms selected from the group comprising nitrogen, oxygen, and sulfur and with the remainder being carbon. Heteroaryl is preferably a monocyclic system with 5 or 6 atoms in total, of which 1-3 are heteroatoms.
“Alkenyl” means an unsaturated radical having up to a maximum of 12 carbon atoms and may be linear or branched with single or multiple branching and containing up to 3 double bonds. Alkenyl is especially lower alkenyl with up to 2 double bonds.
“Alkanoyl” means alkylcarbonyl, and is especially lower alkylcarbonyl.
Halogenated lower alkyl, halogenated lower alkoxy and halogenated lower alkylthio are substituents in which the alkyl moieties are substituted either partially or in full with halogens, preferably with chlorine and/or fluorine and most preferably with fluorine. Examples of such substituents are trifluoromethyl, trifluoromethoxy, trifluoromethylthio, 1,1,2,2-tetrafluoroethoxy, dichloromethyl, fluoromethyl and difluoromethyl.
When a substituent is named as a string of fragments such as “phenyl-lower alkoxycarbonyl-substituted alkylamino,” it is understood that the point of attachment is to the final moiety of that string (in this case amino) and that the other fragments of that string are connected to each other in sequence as they are listed in the string. Thus an example of “phenyl-lower alkoxycarbonyl-substituted alkylamino” is:
When a substituent is named as a string of fragments with a bond at the start (typically written as a dash) such as “—S(O)p (optionally substituted heteroarylalkyl)”, it is understood that the point of attachment is to the first atom of that string (in this case S or sulfur) and that the other fragments of that string are connected to each other in sequence as they are listed in the string. Thus an example of “—S(O)p (optionally substituted heteroarylalkyl)” is:
It is to be understood that the left-most moiety of each of the variants of the linker Y is connected to the ring containing A, B, D, E, and L and that the right-most moiety of the linker is connected to the pyridazine fragment of the generalized formulae. Thus, examples of the use of the linker “—CH2—O—” or of the linker “—O—CH2-” are represented in the following invention compounds:
In generalized structural formula (I), the preferred and most preferred groups are as follows.
R1 and R2 preferably:
wherein
each T2 independently represents N, CH, or CG1;
T3 represents S, O, CH2, or NR3; and
with the proviso that when T3 is O or S, at least one T2 is CH or CG1.
Most preferably, any group G1 is located on a non-terminal atom of the bridge. Most preferably, in the bridge in iii), the terminal T2 is N or CH, the non-terminal T2 is CH or CG1, and T3 is S or O.
The subscript m is preferably 0 or an integer 1-2, and substituents G1 are preferably selected from the group consisting of —N(R6)2; —NR3COR6; halogen; lower alkyl; hydroxy-substituted alkyl; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; hydroxy-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —NO2; —CN; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; optionally substituted heteroarylalkyloxy; and —S(O)p (optionally substituted heteroarylalkyl). Most preferably, m is 0, and G1 is a substituent independently selected from the group consisting of —N(R6)2; —NR3COR6; halogen; —OR6 wherein R6 represents lower alkyl; —NO2; optionally substituted heteroaryloxy; and optionally substituted hetero-arylalkyloxy.
When R6 is an alkyl group, it is preferably lower alkyl. The group R4 is preferably H; p is preferably 0 or 1; and X is preferably NR3.
In the linker group Y, the subscripts n and s are preferably 0 or 1, most preferably O. Preferably, Y is selected from the group consisting of lower alkylene, —CH2—O—; —CH2—S—; —CH2—NH—; —S—; —NH—; —(CR42)n—S(O)p-(5-membered heteroaryl)-(CR42)s—; —(CR42)n—C(G2)(R4)—(CR42)s—; and —O—CH2—. Most preferably, Y is selected from the group consisting of —CH2—O—; —CH2—NH—; —S—; —NH—; —(CR42)n—S(O)p-(5-membered heteroaryl)-(CR42)s—; and —O—CH2—.
In the ring at the left side of the structure (I), A, D, B, and E are preferably CH, and L is N or CH, with the proviso that when L is N, any substituents G3 are preferably monovalent, and when L is CH then any substituents G3 are preferably divalent.
The substituents G3 are preferably selected from the group consisting of monovalent moieties lower alkyl; —NR3COR6; —OR6; —SR6; —S(O)R6; —S(O)2R6; —CO2R6; —CON(R6)2; —S(O)2N(R6)2; —CN; optionally substituted aryl; optionally substituted heteroaryl; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroarylalkyl); and bivalent bridge of structure T2=T2−T3 wherein T2 represents N or CH. T3 is preferably S, O, CR42, or NR3.
Most preferably, G3 is selected from the group consisting of monovalent moieties lower alkyl; —NR3COR6; —CO2R6; —CON(R6)2; —S(O)2N(R6)2; and bivalent bridge of structure T2=T2−T3 wherein T2 represents N or CH. Most preferably T3 is S, O, CH2, or NR3.
Most preferably, the subscript q, which represents the number of substituents G3, is 1.
Ring J is preferably a phenyl ring, and subscript q′ representing the number of substituents G4 on the phenyl ring, is preferably 0, 1, 2, or 3. Subscript q′ is most preferably 1, or 2.
G4 moieties are preferably selected from the group consisting of —N(R6)2; —NR3COR6; halogen; alkyl; halogen-substituted alkyl; hydroxy-substituted alkyl; carboxy-substituted alkyl; lower alkoxycarbonyl-substituted alkyl; amino-substituted alkylamino; N-lower alkylamino-substituted alkylamino; N,N-di-lower alkylamino-substituted alkylamino; N-lower alkanoylamino-substituted alkylamino; hydroxy-substituted alkylamino; carboxy-substituted alkylamino; lower alkoxy-carbonyl-substituted alkylamino; phenyl-lower alkoxycarbonyl-substituted alkylamino; —OR6; —SR6; —S(O)R6; —S(O)2R6; halogenated lower alkoxy; halogenated lower alkylthio; halogenated lower alkylsulfonyl; —OCOR6; —COR6; —CO2R6; —CON(R6)2; —CH2OR3; —NO2; —CN; optionally substituted heteroarylalkyl; optionally substituted heteroaryloxy; —S(O)p (optionally substituted heteroaryl); optionally substituted heteroarylalkyloxy; —S(O)p (optionally substituted heteroaryl-alkyl); as well as
fused ring-forming bridges attached to and connecting adjacent positions of the phenyl ring, said bridges having the structures:
Alkyl groups which constitute all or part of a G4 moiety are preferably lower alkyl.
When G4 is an alkyl group located on ring J adjacent to the linkage —(CR42)p—, and X is NR3 wherein R3 is an alkyl substituent, then G4 and the alkyl substituent R3 on X may be joined to form a bridge of structure —(CH2)p′— wherein p′ is preferably 2 or 3, with the proviso that the sum of p and p′ is 2 or 3, resulting in formation of a nitrogen-containing ring of 5 or 6 members. Most preferably, the sum of p and p′ is 2, resulting in formation of a 5-membered ring.
Most preferably, in G1, G2, G3, and G4, when two groups R6 are each alkyl and located on the same N atom they may be linked by a bond, an O, an S, or NR3 to form a N-containing heterocycle of 5-6 ring atoms.
Preferably, when an aryl, heteroaryl, or heterocyclyl ring is optionally substituted, that ring may bear up to 2 substituents which are independently selected from the group consisting of amino, mono-loweralkyl-substituted amino, di-loweralkyl-substituted amino, lower alkanoylamino, halo-geno, lower alkyl, halogenated lower alkyl, hydroxy, lower alkoxy, lower alkylthio, halogenated lower alkoxy, halogenated lower alkylthio, —CH2OR3, nitro, and cyano.
The method of the invention is intended to be employed for treatment of VEGF-mediated conditions in both humans and other mammals.
The compounds may be administered orally, dermally, parenterally, by injection, by inhalation or spray, or sublingually, rectally or vaginally in dosage unit formulations. The term ‘administered by injection’ includes intravenous, intraarticular, intramuscular, subcutaneous and parenteral injections, as well as use of infusion techniques. Dermal administration may include topical application or transdermal administration. One or more compounds may be present in association with one or more non-toxic pharmaceutically acceptable carriers and if desired, other active ingredients.
Compositions intended for oral use may be prepared according to any suitable method known to the art for the manufacture of pharmaceutical compositions. Such compositions may contain one or more agents selected from the group consisting of diluents, sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide palatable preparations.
Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. These excipients may be, for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; and binding agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. These compounds may also be prepared in solid, rapidly released form.
Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.
Aqueous suspensions containing the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions may also be used. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxypropyl-methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl, p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.
Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example, sweetening, flavoring and coloring agents, may also be present.
The compounds may also be in the form of non-aqueous liquid formulations, e.g., oily suspensions which may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or peanut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
Pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oil phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening and flavoring agents.
Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
The compounds may also be administered in the form of suppositories for rectal or vaginal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal or vaginal temperature and will therefore melt in the rectum or vagina to release the drug. Such materials include cocoa butter and polyethylene glycols.
Compounds of the invention may also be administered transdermally using methods known to those skilled in the art (see, for example: Chien; “Transdermal Controlled Systemic Medications”; Marcel Dekker, Inc.; 1987. Lipp et al. WO 94/04157 3 Mar. 1994). For example, a solution or suspension of a compound of Formula I in a suitable volatile solvent optionally containing penetration enhancing agents can be combined with additional additives known to those skilled in the art, such as matrix materials and bacteriocides. After sterilization, the resulting mixture can be formulated following known procedures into dosage forms. In addition, on treatment with emulsifying agents and water, a solution or suspension of a compound of Formula I may be formulated into a lotion or salve.
Suitable solvents for processing transdermal delivery systems are known to those skilled in the art, and include lower alcohols such as ethanol or isopropyl alcohol, lower ketones such as acetone, lower carboxylic acid esters such as ethyl acetate, polar ethers such as tetrahydrofuran, lower hydrocarbons such as hexane, cyclohexane or benzene, or halogenated hydrocarbons such as dichloromethane, chloroform, trichlorotrifluoroethane, or trichlorofluoroethane. Suitable solvents may also include mixtures one or more materials selected from lower alcohols, lower ketones, lower carboxylic acid esters, polar ethers, lower hydrocarbons, halogenated hydrocarbons.
Suitable penetration enhancing materials for transdermal delivery systems are known to those skilled in the art, and include, for example, monohydroxy or polyhydroxy alcohols such as ethanol, propylene glycol or benzyl alcohol, saturated or unsaturated C8-C18 fatty alcohols such as lauryl alcohol or cetyl alcohol, saturated or unsaturated C8-C18 fatty acids such as stearic acid, saturated or unsaturated fatty esters with up to 24 carbons such as methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl isobutyl tert-butyl or monoglycerin esters of acetic acid, capronic acid, lauric acid, myristinic acid, stearic acid, or palmitic acid, or diesters of saturated or unsaturated dicarboxylic acids with a total of up to 24 carbons such as diisopropyl adipate, diisobutyl adipate, diisopropyl sebacate, diisopropyl maleate, or diisopropyl fumarate. Additional penetration enhancing materials include phosphatidyl derivatives such as lecithin or cephalin, terpenes, amides, ketones, ureas and their derivatives, and ethers such as dimethyl isosorbid and diethyleneglycol monoethyl ether. Suitable penetration enhancing formulations may also include mixtures one or more materials selected from monohydroxy or polyhydroxy alcohols, saturated or unsaturated C8-C18 fatty alcohols, saturated or unsaturated C8-C18 fatty acids, saturated or unsaturated fatty esters with up to 24 carbons, diesters of saturated or unsaturated dicarboxylic acids with a total of up to 24 carbons, phosphatidyl derivatives, terpenes, amides, ketones, ureas and their derivatives, and ethers.
Suitable binding materials for transdermal delivery systems are known to those skilled in the art and include polyacrylates, silicones, polyurethanes, block polymers, styrene-butadiene coploymers, and natural and synthetic rubbers. Cellulose ethers, derivatized polyethylenes, and silicates may also be used as matrix components. Additional additives, such as viscous resins or oils may be added to increase the viscosity of the matrix.
For all regimens of use disclosed herein for compounds of Formula I, the daily oral dosage regimen will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily dosage for administration by injection, including intravenous, intramuscular, subcutaneous and parenteral injections, and use of infusion techniques will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily rectal dosage regimen will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily vaginal dosage regimen will preferably be from 0.01 to 200 mg/Kg of total body weight. The daily topical dosage regimen will preferably be from 0.1 to 200 mg administered between one to four times daily. The transdermal concentration will preferably be that required to maintain a daily dose of from 0.01 to 200 mg/Kg. The daily inhalation dosage regimen will preferably be from 0.01 to 10 mg/Kg of total body weight.
It will be appreciated by those skilled in the art that the particular method of administration will depend on a variety of factors, all of which are considered routinely when administering therapeutics. It will also be understood, however, that the specific dose level for any given patient will depend upon a variety of factors, including, but not limited to the activity of the specific compound employed, the age of the patient, the body weight of the patient, the general health of the patient, the gender of the patient, the diet of the patient, time of administration, route of administration, rate of excretion, drug combinations, and the severity of the condition undergoing therapy. It will be further appreciated by one skilled in the art that the optimal course of treatment, i.e., the mode of treatment and the daily number of doses of a compound of Formula I or a pharmaceutically acceptable salt thereof given for a defined number of days, can be ascertained by those skilled in the art using conventional treatment tests.
General Preparative Methods
The compounds of the invention may be prepared by use of known chemical reactions and procedures. Nevertheless, the following general preparative methods are presented to aid the reader in synthesizing the KDR inhibitors, with more detailed particular examples being presented below in the experimental section describing the working examples.
All variable groups of these methods are as described in the generic description if they are not specifically defined below. When a variable group or substituent with a given symbol (i.e. R3, R4, R6, G1, G2, G3, or G4) is used more than once in a given structure, it is to be understood that each of these groups or substituents may be independently varied within the range of definitions for that symbol. As defined above, the compounds of the invention contain ring units each of which may independently bear between 0 and 5 substituents G1, G3, or G4, which are not defined as H. By contrast, it is to be noted that in the general method schemes below, the G1, G3, or G4 substituents are used as if their definition includes H, to show where such G1, G3, or G4 substituents may exist in the structures, and for ease in drawing. No change in the definition of G1, G3, or G4 is intended by this non-standard usage, however. Thus, only for purposes of the general method schemes below, G1, G3, or G4 may be H in addition to the moieties set forth in the definitions of G1, G3, or G4. The ultimate compounds contain 0 to 5 non-hydrogen groups G1, G3, or G4.
Within these general methods the variable M is equivalent to the moiety
in which each variable group or substituent is allowed to independently vary within the limits defined earlier for that symbol.
Within these general methods the variable Q1 is equivalent to the moiety
in which L is N and each other variable group or substituent is allowed to independently vary within the limits defined earlier for that symbol.
Within these general methods the variable Q2 is equivalent to the moiety
in which each variable group or substituent is allowed to independently vary within the limits defined earlier for that symbol.
It is recognized that compounds of the invention with each claimed optional functional group cannot be prepared with each of the below-listed methods. Within the scope of each method optional substituents are used which are stable to the reaction conditions, or the functional groups which may participate in the reactions are present in protected form where necessary, and the removal of such protective groups is completed at appropriate stages by methods well known to those skilled in the art.
General Method A—The compounds of formula I-A in which X, M, and Q2 are defined as above, Y is —CH2—O—, —CH2—S—, —CH2—NH—, —O—, —S—, or —NH—, and R1 and R2 together with the carbons to which they are attached form a fused 5-membered ring aromatic heterocycle, hal is halogen (Cl, Br, F, or I but preferably Cl, Br or F) are conveniently prepared according to a reaction sequence as shown in Method A. Thus, a heterocycle of formula II in which R is lower alkyl can be made by one skilled in the art according to the corresponding published procedures in the reference table. In the cases of thiophene-2,3-dicarboxylic acid (table entry 1) and pyrazole-3,4-dicarboxylic acid (table entry 10), the carboxylic acids are converted to methyl or ethyl esters by treatment with the corresponding alcohol and catalytic mineral acid (typically sulfuric acid) at reflux. The diester of formula II is treated with hydrazine hydrate to furnish intermediate III (for specific reaction conditions see Robba, M.; Le Guen, Y. Bull. Soc. Chem. Fr. 1970 12 4317). Compound III is treated with a halogenating agent such as phosphorous oxychloride, phosphorous oxybromide, phosphorous pentabromide, or phosphorous pentachloride to yield dihalo intermediate IV. The dichloro or dibromo intermediates can be converted to the difluoro intermediate (when desired) by reaction with hydrogen fluoride. By using iodo reagents such as potassium iodide or tetrabutyl-ammonium iodide in subsequent steps, the iodo intermediate is formed in the reaction mixtures without being isolated as a pure substance. Dihalo intermediate IV is treated with a nucleophile of formula V in refluxing alcohol or other suitable solvent such as tetrahydrofuran (THF), dimethoxyethane (DME), dimethylformamide (DMF), dimethylsulfoxide (DMSO), or the like to furnish the intermediate of formula VI. Such condensations can also be done in a melt free of solvent and can be catalyzed by acids such as HCl or bases such as triethylamine or 1,8-diazobicyclo[5.4.0]undec-7-ene (DBU). The compound of formula VI is reacted with compounds of formula VII in a suitable aprotic solvent such as DMSO, DMF or solvent free often with a basic catalyst such as DBU or CsCO4, or a crown ether such as 18-crown-6 at temperatures usually between room temperature and reflux to furnish invention compound of formula I-A. It is understood that the nature of the starting materials will dictate the choice of suitable solvents, catalyst (if used) and temperature by one skilled in the art. Intermediates of formula V and VII are often commercial or are conveniently prepared by methods well known to those skilled in the art. For example see Martin, I., et al. Acta. Chem. Scand. 1995 49 230 for the preparation of VII in which Y is —CH2—O— and Q2 is 4-pyridyl substituted by a 2-aminocarbonyl group (2-CONH2).
General Method B—The compounds of formula I-B in which M, X, and Q2 are as defined above and Y is —CH2—O—, —CH2—S—, —CH2—NH—, —O—, —S—, or —NH— are conveniently prepared as shown in Method B. According to a procedure described in the literature (Tomisawa and Wang, Chem. Pharm. Bull., 21, 1973, 2607, 2612), isocarbostyril VIII is reacted with PBr5 in a melt to form 1,4-dibromoisoquinoline IX. Intermediate IX is treated with a nucleophile of formula V in refluxing alcohol to furnish intermediate of formula X. Such condensations can also be done in a melt free of solvent and can be catalyzed by acids such as HCl or bases such as triethylamine or 1,8-diazobicyclo[5.4.0]undec-7-ene (DBU). The compound of formula X is reacted with compounds of formula VII in a suitable aprotic solvent such as DMSO, DMF or solvent free often with a basic catalyst such as DBU or CsCO4 at elevated temperatures to furnish invention compound of formula I-B. This method is most useful when Y is —C1-12—S— or —S—.
General Method C—The compounds of formula I-C in which M, X, R1, R2, m and Q2 are defined as above are conveniently prepared according by a reaction sequence as shown in method C. In this method m is preferably 0 and R1 and R2 together with the carbons to which they are attached form a fused benzene or fused 5-member ring aromatic heterocycle. Starting material XI is either commercial or is prepared by one skilled in the art as shown in the reference table below. Starting material XI is reacted with urea or ammonia, usually at elevated temperature and pressure (in the case of ammonia), to form imide XII. The imide is reacted with an aldehyde XIII in acetic acid and piperidine at reflux to yield intermediate XIV. Reaction of XIV with sodium borohydride in methanol or other suitable solvents according to the general procedure described by I. W. Elliott and Y. Takekoshi (J. Heterocyclic Chem. 1976 13, 597) yields intermediate XV. Treatment of XV with a suitable halogenating agent such as POCl3, POBr3, PCl5, PBr5 or thionyl chloride yields halo intermediate XVI which is reacted with nucleophile of formula V in refluxing alcohol to furnish invention compound of formula I-C. Such condensations can also be done in a melt free of solvent and can be catalyzed by acids such as HCl or bases such as triethylamine or 1,8-diazobicyclo[5.4.0]undec-7-ene (DBU). Alternatively, reagent V can be condensed with intermediate XV be heating the two components with P2O5 in a melt to yield invention compound of structure I-C. This last method is especially effective when X is an amine linker.
General Method D—The compounds of formula I-D-1 in which R1, R2, R6, M, X, Y, G3 and Z are defined as above and q is 0 or 1 are conveniently prepared via a reaction sequence as shown in Method D. Thus, pyridine substituted pyridazines or pyridines (I-D-1) are functionalized into substituted 2-aminocarbonyl pyridines of formula (I-D-2) by the use of formamides (XVII) in the presence of hydrogen peroxide and iron salts, according to a procedure described in the literature (Minisci et al., Tetrahedron, 1985, 41, 4157). This method works best when R1 and R2 together constitute a fused aromatic heterocycle or fused aromatic carbocycle. In those cases that Z is CH and R1 and R2 do not form a fused aromatic, an isomeric side product in which Z is CCONHR6 can be formed and, if so formed, is removed from the desired product by chromatography.
General Method E—The compounds of formula I-E-1 and I-E-2 in which R1, R2, R6, M, X, Y, G3, and Z are defined as above, q is 0 or 1, and R3 is lower alkyl are conveniently prepared via a reaction sequence as shown in Method E. Thus, pyridine substituted pyridazines or pyridines (I-D-1) are functionalized into substituted 2-alkoxycarbonyl pyridines of formula (I-E-1) by the use of monoalkyloxalates (XVIII) in the presence of S2O8−2, acid and catalytic amounts of AgNO3, according to a procedure described in the literature (Coppa, F. et al., Tetrahedron Letters, 1992, 33 (21), 3057). Compounds of formula I-E-1 in which R3 is H are then formed by hydrolysis of the ester with a base such as sodium hydroxide in methanol/water. Compounds of formula I-E-2 in which the R6 groups are independently defined as above, but especially including those compounds in which neither R6 is H, are conveniently prepared from the acid (I-E-1, R3═H) by treatment with amine XIX in the presence of a coupling agent such as DCC (dicyclohexylcarbodiimide). This method works best when R1 and R2 together constitute a fused aromatic heterocycle or fused aromatic carbocycle. In those cases that Z is CH and R1 and R2 do not form a fused aromatic, an isomeric side product in which Z is CCO2R3 can be formed in the first step and, if so formed, is removed from the desired product by chromatography.
General Method F—The compounds of formula I-F in which M, Q2 and X are defined as above, m is an integer of 1-5, and R1 and R2 together with the carbons to which they are attached form a fused 5-membered ring aromatic heterocycle can be prepared via a reaction sequence as shown in method F. The readily available heterocyclylcarboxylic acid starting material XX is reacted with butyl lithium followed by dimethylformamide to yield the aldehyde with structure XXI. Reaction of XXI with hydrazine yields pyridazinone XXII. Treatment of XXII with a suitable halogenating agent such as POCl3, POBr3, PCl5, PBr5 or thionyl chloride yields a halo intermediate which is reacted with nucleophile of formula V in refluxing alcohol to furnish intermediate compound of formula XXIII. Such condensations can also be done in a melt free of solvent and can be catalyzed by acids such as HCl or bases such as triethylamine or 1,8-diazobicyclo[5.4.0]undec-7-ene (DBU). Alternatively, reagent V can be condensed with intermediate XXII be heating the two components with P2O5 in a melt to yield XXII. This last method is especially effective when X is an amine linker. Formation and alkylation of the Reissert compound XXIII with halide XXIV is done as described by the general method of F. D. Popp, Heterocycles, 1980, 14, 1033 to yield the intermediate of structure XXV. Treatment of XXV with base then yields invention compound I-F.
General Method G—The compounds of formula I-G in which M, Q2 and X are defined as above, m is an integer of 1-4, and R1 and R2 together with the carbons to which they are attached form a fused 5-membered ring aromatic heterocycle can be prepared via a reaction sequence as shown in method G. Aldehyde XXI, from method F, can be reduced with sodium borohydride to yield a hyroxyacid which is lactonized using methods well known to those skilled in the art such as with toluenesulfonyl chloride to yield lactone XXVI. Condensation of intermediate XXVI with aldehyde XIII in the presence of a base such as sodium methoxide usually in a solvent such as methanol under reflux yields an intermediate of structure XXVII. Reaction of XXVII with hydrazine or preferably with hydrazine hydrate at a temperature of 100-150° C. leads to an intermediate of structure XXVIII. Conversion of intermediate XXVIII to invention compound of structure I-G is done by methods as described in method C by using XXVIII rather than XV.
General Method H—The compounds of formula I-H in which the R1, R2, M, X, R6, q and G3 are defined as above are conveniently prepared via a reaction sequence as shown in Method H. Thus the methods described in Martin, I; Anvelt, J.; Vares, L.; Kuehn, I.; Claesson, A. Acta Chem. Scand. 1995, 49, 230-232 or those of methods D or E above by substituting readily available pyridine-4-carboxylic ester XXX for I-D-1 are used to convert XXX into XXXI. Reduction of the ester as described by Martin, et al. above is next done with a mild reducing agent such as NaBH4 such that the amide substituent is left unchanged to yield alcohol XXXII. This alcohol is then heated with a base such as DBU or CsCO4 with halopyridazine VI from method A under anhydrous conditions to yield the invention compound with formula I-H.
General Method I—Invention compounds having formula I-I in which the R1, R2, M, X, R6, q, and G3 are defined as above and W is a bond or —CH2— are conveniently prepared via a reaction sequence as shown in Method I. This method is especially useful when q is 1 and XXXIII is 4-chloropyridine. Alternatively, other 4-halopyridines such as 4-fluoropyridine or 4-bromopyridine can be used in this process. Thus readily available 4-halopyridines XXXIII are converted to intermediates of formula XXXIV by using the general procedures of methods D or E above by substituting the 4-halopyridine for I-D-1. Reaction of XXXIV with either potassium or sodium hydrogen sulfide yields a thiol having formula XXXV. Alternatively, the alcohol function of intermediate XXXII from method H is converted to a leaving group by reaction with methanesulfonyl chloride and a suitable base such as triethylamine in the cold such that polymeric material formation is minimized and the resultant intermediate is reacted with either potassium or sodium hydrogen sulfide to yield a thiol having formula XXXVI. Either thiol have formula XXXV or formula XXXVI is reacted with intermediate VI from method A and a suitable base such as diisopropylethylamine or CsCO4 in DMF or other suitable anhydrous solvent or in the absence of solvent to yield I-D-9.
General Method J—Invention compounds such as those having formula I-J-1 or I-J-2 in which the R1, R2, M, X, W, and G3 are defined as above and having a sulfoxide or sulfone within the structure are conveniently prepared via a reaction sequence as shown in Method J. Reaction of compounds of this invention that contain a thioether group either as part of a substituent G1, G3, or G4 or as part of Y as shown in the representative structure I-I from Method I can be converted to the invention compounds with a sulfoxide moiety such as I-J-1 by treatment with one equivalent of m-chloroperbenzoic acid in methylene chloride or chloroform (MCPBA, Synth. Commun., 26, 10, 1913-1920, 1996) or by treatment with sodium periodate in methanol/water at between 0° C. and room temperature (J. Org. Chem., 58, 25, 6996-7000, 1993). The expected side products consisting of mixtures of various N oxides and the sulfone I-J-2 can be removed by chromatography. The sulfone I-J-2 is obtained by the use of an additional equivalent of MCPBA or preferably by use of potassium permanganate in acetic acid/water (Eur. J. Med. Chem. Ther., 21, 1, 5-8, 1986) or by use of hydrogen peroxide in acetic acid (Chem. Heterocycl. Compd., 15, 1085-1088, 1979). In those cases that unwanted N oxides become a significant product, they can be converted back to the desired sulfoxides or sulfones by hydrogenation in ethanol/acetic acid with palladium on carbon catalysts (Yakugaku Zasshi, 69, 545-548, 1949, Chem. Abstr. 1950, 4474).
General Method K—Invention compounds having formula I-K in which the R1, R2, M, X, and Q1 are defined as above are conveniently prepared via a reaction sequence as shown in Method K. One skilled in the art prepares starting materials of structure XXXVII by methods known in the literature. For example XXXVII wherein R1 and R2 together with the carbons to which they are attached form a 2,3-substituted thiophene, furan, pyrrole, cyclopentadienyl, oxazole or thiazole are prepared using the general chemistry given in J. Org. Chem., 1981, 46, 211 and hydrolizing the initially formed tert-butyl ester with trifluoroacetic acid. The pyrazole starting material can be prepared by reacting 2-oxo-3-pentyn-1,5-dioic acid (J. Chem. Phys. 1974, 60, 1597) with diazomethane. The starting material wherein R1 and R2 together with the carbons to which they are attached form a phenyl are prepared by the methods of Cymerman-Craig et al., Aust. J. Chem. 1956, 9, 222, 225. Compounds of formula XXXVII in which R1 and R2 are lower alkyl are conveniently prepared according to procedures given in patent CH 482415 (Chem. Abstr. 120261u, 1970). The crude diacid of formula XXXVII is subsequently treated with hydrazine to furnish pyridazinone XXXVIII (for specific reaction conditions see Vaughn, W. R.; Baird, S. L. J. Am. Chem. Soc. 1946 68 1314). Pyridazinone XXXVIII is treated with a chlorinating agent such as phosphorous oxychloride to yield an intermediate dichloro species which undergoes hydrolysis upon aqueous workup to furnish chloropyridazine XXXIX. Chloro acid XXXIX is treated with a nucleophile of formula V in the presence of a base such as sodium hydride in a solvent such as DMF or in the absence of a solvent. The resultant acid XXXX is reduced with a reducing agent such as BH3.THF according to the procedure of Tilley, J. W.; Coffen D. L. Schaer, B. H.; Lind, J. J. Org. Chem. 1987 52 2469. Product alcohol XXXXI is reacted with a base and optionally substituted 4-halo-pridyl, optionally substituted 4-halo-pyrimidyl or optionally substituted 4-halo-pyridazyl (XXXVII) to furnish invention compound of formula I-K (for specific reaction conditions see Barlow, J. J.; Block, M. H.; Hudson, J. A.; Leach, A.; Longridge, J. L.; Main, B. g.; Nicholson, S. J. Org. Chem. 1992 57 5158).
General Method L—Invention compounds having formula I-L in which the R1, R2, M, X, and Q1 are defined as above are conveniently prepared via a reaction sequence as shown in Method L. Thus alcohol of formula XXXXI from method K is reacted with methanesulfonyl chloride in the presence of a suitable base followed by potassium or sodium hydrogen sulfide to yield thiol XXXXIII. The thiol is then reacted with 4-halopyridine XXXXII from method K in the presence of a suitable base such as triethylamine to yield invention compound I-K. Alternatively, XXXXI is converted to halo intermediate of formula XXXXIV by methods well known to those skilled in the art and the halide is reacted with thiol XXXXV to yield I-K. Intermediate XXXXIV can also be converted to intermediate XXXXIII by treatment with KHS or NaHS. Reagents XXXXV are either commercially available such as 4-mercaptopyridine or can be prepared by one skilled in the art such as by method I above.
Further Therapeutic Agents
The compounds of formula I according to the present invention can be combined with further therapeutic agents presently used to treat, prevent or manage pulmonary hypertension such as, but not limited to, anticoagulants, diuretics, cardiac glycosides, calcium channel blockers, vasodilators, prostacyclin analogues, endothelium antagonists, phosphodiesterase inhibitors, endopeptidase inhibitors, lipid lowering agents, thromboxane inhibitors and other therapeutics known to reduce pulmonary artery pressure.
Examples of anticoagulants include, but are not limited to, e.g. warfarin useful in the treatment of patients with pulmonary hypertension having an increased risk of thrombosis and thromboembolism.
Examples of calcium channel blockers include, but are not limited to, diltiazem, felodipine, amlodipine and nifedipine particularly useful for vasoreactive patients at right heart catheterization.
Examples of vasodilators include, but are not limited to, e.g. prostacyclin, epoprostenol, treprostinil and nitric oxide (NO).
Examples of phosphodiesterase inhibitors include, but are not limited to, particularly phospho-diesterase V inhibitors such as e.g. tadalafil, sildenafil and vardenafil.
Examples of endothelin antagonists include, but are not limited to, e.g. bosentan and sitaxentan, preferably bosetan.
Examples of prostacyclin analogues include, but are not limited to, e.g. ilomedin, treprostinil and epoprostenol.
Examples of lipid lowering agents include, but are not limited to, e.g. HMG CoA reductase inhibitors such as simvastatin, pravastatin, atorvastatin, lovastatin, itavastatin, fluvastatin, pitavastatin, rosuvastatin, ZD-4522 and cerivastatin
Examples diuretics include, but are not limited to, e.g. chlorthalidon, indapamid, bendro-flumethiazid, metolazon, cyclopenthiazid, polythiazid, mefrusid, ximapid, chlorothiazid and hydrochlorothiazid particularly useful to manage peripheral edema.
Examples of other therapeutics known to reduce pulmonary artery pressure include, but are not limited to, e.g. ACE inhibitors such as enalapril, ramipril, captopril, cilazapril, trandolapril, fosinopril, quinapril, moexipril, lisinopril and perindopril, or AT II inhibitors such as losartan, candesartan, irbesartan, embusartan, valsartan and telmisartan, or iloprost, betaprost, L-arginine, omapatrilat, oxygen particularly useful in those patients with resting or exercise-induced hypoxemia or digoxin particularly useful to improve right ventricular function in patients with right ventricular failure.
Furthermore the compounds and combinations of the invention can be combined with kinase inhibitors and/or elastase inhibitors.
Examples of kinase inhibitors include, but are not limited to, e.g. BMS-354825, canertinib, erlotinib, gefitinib, imatinib, lapatinib, lestaurtinib, lonafarnib, pegaptanib, pelitinib, semaxanib, tandutinib, tipifarnib, vatalanib, lonidamine, fasudil, leflunomide, bortezomib, imatinib, erlotinib and glivec. Preference is given to glivec.
Furthermore the compounds and combinations of the invention can be combined with NO-independent and heme-dependent guanylyl cyclase stimulators and/or NO- and heme-independent guanylyl cyclase activators.
Examples of NO-independent and heme-dependent guanylyl cyclase stimulators include, but are not limited to, e.g. compounds described in WO 00/06568, WO 00/06569, WO 02/42301 and WO 03/095451. Preference is given to methyl 4,6-diamino-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]-5-pyrimidinyl(methyl)carbamate and 4,6-diamino-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]-5-pyrimidinylcarbamate.
Examples of NO- and heme-independent guanylyl cyclase activators include, but are not limited to, e.g. compounds described in WO 01/19355, WO 01/19776, WO 01/19778, WO 01/19780, WO 02/070462, WO 02/042301 and WO 02/070510. Preference is given to 4-[((4-Carboxybutyl)-{2-[(4-phenetylbenzyl)oxy]phenethyl}amino)-methyl]benzoic acid.
Indications
The compounds and combinations according to the present invention can be used for manufacture of a medicament for treating, preventing and managing pulmonary hypertension. Also the present invention provides methods of treating, preventing and managing pulmonary hypertension, comprising administering effective amounts of at least one compound of formula I and optionally at least one further therapeutic agent according to the invention. An “effective amount” is the quantity of the compound that is useful to achieve the desired result, e.g., to treat, prevent or manage the disease or condition.
The term “pulmonary hypertension” according to the invention include, but is not limited to, primary pulmonary hypertension, secondary pulmonary hypertension, familial pulmonary hyper-tension, sporadic pulmonary hypertension, precapillary pulmonary hypertension, pulmonary arterial, pulmonary artery hypertension, idiopathic pulmonary hypertension, thrombotic pulmonary arteriopathy, plexogenic pulmonary arteriopathy and pulmonary hypertension associated with or related to, left ventricular dysfunction, mitral valvilar disease, constrictivepericarditis, aortic stenosis, cardiomyopathy, mediastinal fibrosis, anomalous pulmonary venous drainage, pulmonary venoocclusive disease, collagen vascular disease, congenital heart disease, congenital heart disease, pulmonary venus hypertension, chronic obstructive pulmonary disease, interstitial lung disease, sleep-disordered breathing, alveolarhyperventilation disorder, chronic exposure to high altitude, neonatal lung disease, alveolar-capillary dysplasia, sickle cell disease, other coagulation disorders, chronic thromboemboli, connective tissue disease, lupus, schistosomiasis, sarcoidosis or pulmonary capillary hemangiomatosis.
Any form of pulmonary hypertension can be treated in accordance with the present invention, including, but not limited to, mild, e.g., associated with increases of mean blood pressure of about 20-30 mm Hg at rest; moderate, e.g., associated with increases of 30-39 mm Hg at rest; and severe, e.g., associated with increases of 40 mm Hg or more at rest.
Pulmonary hypertension includes pulmonary arterial hypertension (PAH), and includes, primary pulmonary hypertension (PPH), idiopathic PAH (IPAH), familial PAH (FPAH). Several classifications systems for pulmonary hypertension have been published, including the Evian Nomenclature and Classification of pulmonary hypertension (PH) (1998) and the Revised Nomenclature and Classification of PH (2003). See, Lewis et al., Chest, 2004, 126 73-10, which is hereby incorporated by reference in its entirety. Any disease PH listed in these classification schemes can be treated, managed, or prevented in accordance with the present invention. Risk factors and diagnostic criteria for PH are described in McGoon et al., Chest, 126, 14-34, 2004, which is hereby incorporated by reference in its entirety.
The following list is the 2003 classification proposed at the Third World Conference on Pulmonary Hypertension: PAH, IPAH, FPAH, collagen vascular disease, congenital systemic to pulmonary shunts (large, small, repaired or nonrepaired), Portal hypertension, drugs and toxins, other (glycogen storage disease, gaucher disease, hereditary hemorrhagic telangiectasia, hemoglobinopathies, myeloproliferative disorders, splenectomy), associated with significant venous or capillary involvement, pulmonary venous hypertension, pulmonary capillary heman-giomatosis, pulmonary venous hypertension, left-sided atrial ventricular heart disease, left-sided valvular heart disease, pulmonary hypertension associated with hypoxemia, COPD, interstitial lung disease, sleep-disordered breathing, alveolar hypoventilation disorders, chronic exposure to high altitude, PH due to chronic thrombotic and/or embolic disease, thromboembolic obstruction of proximal pulmonary arteries, thromboembolic obstruction of distal pulmonary arteries, pulmonary embolism (tumor, parasites, foreign material), sarcoidosis, histiocytosis X, lymphangiomatosis, compression of pulmonary vessels (adenopathy, tumor, fibrosing mediastinitis)
Any of the above-mentioned disorders can be associated with an increased risk of pulmonary hypertension, including, subjects having, e.g., congenital heart disease (e.g., Eisenmenger syndrome); left heart disease; pulmonary venous disease (e.g., fibrosis tissue narrowing or occluding pulmonary veins and venules); pulmonary arterial disease; diseases causing alveolar hypoxia; fibrotic lung diseases; Williams syndrome; subjects with intravenous drug abuse injury; pulmonary vasculitis (such as Wegener's, Goodpasture's, and Churg-Strauss syndromes); emphysema; chronic bronchitis; kyphoscoliosis; cystic fibrosis; obesity-hyper-ventilation and sleep apnea disorders; pulmonary fibrosis; sarcoidosis; silocosis; CREST (calcinosis cutis, Raynaud phenomenon; esophageal motility disorder; sclerodactyl), and teleangiectasia) and other connective tissue diseases. For example, a subject who possesses a BMPR2 mutation (bone morphogenetic protein receptor II) has a 10-20% lifetime risk of acquiring FPAH. Subjects with hereditary hemorrhagic telangiectasa were also identified as being at risk for IPAH, especially those carrying mutations in ALK1. See, McGoon et al., Chest, 2004, 126, 14-34.
According to the invention the term “treating” refers to the administration of a pharmaceutical composition after the onset of symptoms of pulmonary hypertension, whereas “preventing” refers to the administration prior to the onset of symptoms, particularly to patients at risk of pulmonary hypertension. The term “managing” encompasses preventing the recurrence of pulmonary hypertension in a patient who suffered from pulmonary hypertension.
Administration
Compounds or drug combinations of the present invention can be administered in any form by any effective route, including, e.g., oral, parenteral, enteral, intravenous, intraperitoneal, topical, transdermal (e.g., using any standard patch), ophthalmic, nasally, local, non-oral, such as aerosol, inhalation, subcutaneous, intramuscular, buccal, sublingual, rectal, vaginal, intra-arterial, and intrathecal, etc. They can be administered alone, or in combination with any ingredient(s), active or inactive.
Preference is given to an oral administration.
Compounds or drug combinations of the present invention can be converted in a known manner into the usual formulations, which may be liquid or solid formulations e.g. without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid and liquid aerosols and emulsions.
Examples of solid formulations for oral administration are described in U.S. provisional application No. 60/605,752.
The combinations of the present invention can be administered at any time and in any effective form. For example, the compounds can be administered simultaneously, e.g., as a single composition or dosage unit (e.g., a pill or liquid containing both compositions), or they can be administered as separate compositions, but at the same time (e.g., where one drug is administered intravenously and the other is administered orally or intramuscularly). The drugs can also be administered sequentially at different times. Agents can be formulated conventionally to achieve the desired rates of release over extended period of times, e.g., 12-hours, 24-hours. This can be achieved by using agents and/or their derivatives which have suitable metabolic half-lives, and/or by using controlled release formulations.
The drug combinations can be synergistic, e.g., where the joint action of the drugs is such that the combined effect is greater than the algebraic sum of their individual effects. Thus, reduced amounts of the drugs can be administered, e.g., reducing toxicity or other deleterious or unwanted effects, and/or using the same amounts as used when the agents are administered alone, but achieving greater efficacy.
Compounds or drug combinations of the present invention can be further combined with any other suitable additive or pharmaceutically acceptable carrier. Such additives include any of the substances already mentioned, as well as any of those used conventionally, such as those described in Remington: The Science and Practice of Pharmacy (Gennaro and Gennaro, eds, 20th edition, Lippincott Williams & Wilkins, 2000); Theory and Practice of Industrial Pharmacy (Lachman et al., eds., 3rd edition, Lippincott Williams & Wilkins, 1986); Encyclopedia of Pharmaceutical Technology (Swarbrick and Boylan, eds., 2nd edition, Marcel Dekker, 2002). These can be referred to herein as “pharmaceutically acceptable carriers” to indicate they are combined with the active drug and can be administered safely to a subject for therapeutic purposes.
In addition, compounds or drug combinations of the present invention can be administered with other active agents or other therapies that are utilized to treat any of the above-mentioned diseases and/or conditions.
The present invention provides also combinations of at least one compound of Formula I and at least one other therapeutic agent mentioned above useful in treating a disease or disorder. “Combinations” for the purposes of the invention include:
The dosage of each agent of the combination can be selected with reference to the other and/or the type of disease and/or the disease status in order to provide the desired therapeutic activity. For example, the active agents in the combination can be present and administered in a fixed combination. “Fixed combination” is intended here to mean pharmaceutical forms in which the components are present in a fixed ratio that provides the desired efficacy. These amounts can be determined routinely for a particular patient, where various parameters are utilized to select the appropriate dosage (e.g., type of disease, age of patient, disease status, patient health, weight, etc.), or the amounts can be relatively standard.
The amount of the administered active ingredient can vary widely according to such considerations as the particular compound and dosage unit employed, the mode and time of administration, the period of treatment, the age, sex, and general condition of the patient treated, the nature and extent of the condition treated, the rate of drug metabolism and excretion, the potential drug combinations and drug-drug interactions, and the like.
In another embodiment of the invention the compound of formula I is administered in combination with at least one further therapeutic agent in an amount that those of ordinary skill in the art can determine by their professional judgement.
The pharmaceutical composition according to the invention is administered one or more, preferably up to three, more preferably up to two times per day. Preference is given to an administration via the oral route. With each administration the number of tablets or capsules taken in at the same time should not exceed two.
Nevertheless, it may in some cases be advantageous to deviate from the amounts specified, depending on body weight, individual behaviour toward the active ingredient, type of preparation and time or interval over which the administration is effected. For instance, less than the aforementioned minimum amounts may be sufficient in some cases, while the upper limit specified has to be exceeded in other cases. In the case of administration of relatively large amounts, it may be advisable to divide these into several individual doses over the day.
The combination can comprise effective amounts of at least one compound of Formula I and at least one other therapeutic agent mentioned above, which achieves a greater therapeutic efficacy than when either compound is used alone. The combination can be useful to treat, prevent or manage pulmonary hypertension, where the therapeutic effect is not observed when the agents are used alone, or where an enhanced effect is observed when the combination is administered.
The relative ratios of each compound in the combination can also be selected based on their respective mechanisms of action and the disease biology. The relative ratios of each compound can vary widely and this invention includes combinations for treating, preventing or managing pulmonary hypertension where the amounts of the formula I compound and the other therapeutic agent can be adjusted routinely such that either is present in higher amounts.
The release of one or more agents of the combination can also be controlled, where appropriate, to provide the desired therapeutic activity when in a single dosage form, combination pack, kit or when in separate independent dosage forms.
Preference is given to a combination comprising a compound of formula I and at least one compound selected from the group consisting of phosphodiesterase V inhibitors, endothelin antagonists, prostacyclin analogues, kinase inhibitors and elastase inhibitors. More preferably a combination comprising 4-(4-chlorophenylamino)-7-(2-aminocarbonyl-4-pyridylmethoxy)furo-[2,3-d]pyridazine and at least one compound selected from the group consisting of tadalafil, sildenafil, vardenafil, bosentan, sitaxentan, ilomedin, treprostinil and epoprostenol is used. Most preferably a combination comprising 4-(4-chlorophenylamino)-7-(2-aminocarbonyl-4-pyridylmethoxy) furo-[2,3-d]pyridazine and bosentan or vardenafil is used.
Step 1: Preparation of Intermediate A: A mixture of 2.90 g, 19.07 mMol of isocarbostyril and 14.40 g, 33.68 mMol of phosphorus pentabromide were allowed to melt together at 140° C. The melt turned into a red liquid and after about 10 minutes the reaction mixture solidified and was cooled. The reaction mixture was crushed up and dumped into ice water. The resulting solid was filtered and air-dried. wt. 5.50 g, 96% yield, mp.=94-96°. Rf=0.66 in 40% ethyl acetate in hexanes.
Step 2: A mixture of 1.00 g, 3.49 mMol of 1,4-dibromoisoquinoline (Intermediate A) from step 1 and 4-chloroaniline were melted together at 140°. The reaction mixture turned into a deep red liquid and after about 10 minutes the reaction mixture solidified and was done. The reaction mixture was broken up and triturated with a 50/50 methanol/THF mixture then filtered and air dried without further purification. wt. 0.75 g, 64.4%, mp.=260-263°. Rf=0.58 in 40% ethyl acetate in hexanes.
Step 3: A mixture of 0.05 g, 0.1498 mMol of 1-(4-chloroaniline)-4-bromoisoquiniline and 0.02 g, 0.18 mMol of 4-mercaptopyridine were combined and melted together at 140° for about 10 minutes. The resulting reaction mixture was purified on a 1000 micron prep plate using 5% methanol in hexanes as the solvent. wt. 0.0103 g, 19% yield, mp. 192-195°. Rf=0.50 in 40% ethyl acetate in hexanes.
The procedure used for the preparation of Example 1 was used to prepare the title compound by substituting 5-aminoindane for 4-chloroaniline in step 2. Mp. 100-103°, TLC Rf 0.40 (40% ethyl acetate in hexanes).
The procedure used for the preparation of Example 1 was used to prepare the title compound by substituting 6-aminobenzothiazole for 4-chloroaniline in step 2.
TLC Rf 0.36 (5% methanol/methylene chloride); MS=387
Step 1: A mixture of homophthalimide (770 mg, 4.78 mmol), 4-pyridinecarboxaldehyde (0.469 mL, 4.78 mmol) and piperidine (0.5 mL) in acetic acid (25 mL) was heated at reflux for 1 h. The resultant solution was cooled to room temperature. The solid product was removed by filtration, washed by water (4×10 mL) and dried under vacuum to afford 920 mg (3.67 mmol, 77% yield) of a mixture of Z and E isomers of the above compound. 1H-NMR (DMSO-d6) complex proton signals shown in aromatic region due to existence of both E and Z isomers. MS ES 251 (M+H)+, 252 (M+2H)+.
Step 2: To a suspension of starting material (1.70 g, 6.8 mmol) in methanol (250 mL) at 0° C. was added slowly sodium borohydride (3.0 g, 79 mmol). The mixture was allowed warmed to rt and continued stirring for 1 hr. The reaction was quenched with water (10 mL) and stirred for 10 minutes. The resulting mixture was concentrated to remove solvent. To the residue was added water with ice (100 mL), and adjusted the pH=2 with 2 N HCl solution. Stirred for 10 minutes, added 2 N NaOH until pH of the solution was about 11. The resulting solution was extracted by CH2Cl2 (4×100 mL). The combined organic layers were collected, dried over MgSO4 and concentrated. The residue was purified by column chromatography (1:10 v/v methanol-dichloromethane) to afford 400 mg of the title compound as a solid (1.70 mmol, yield 25%). 1H-NMR (MeOH-d4) 8.33 to 8.39 (m, 4H), 7.50 to 7.68 (m, 3H), 7.30-7.31 (m, 2H), 7.14 (s, 1H), 4.15 (s, 2H); MS ES 237 (M+H)+, 238 (M+2H); TLC (1:10 v/v methanol-dichloromethane) Rf=0.40.
Step 3: A mixture of 4-chloroaniline (178 mg, 1.40 mmol), phosphorus pentoxide (396 mg, 1.40 mmol) and triethylamine hydrochloride (193 mg, 1.40 mmol) was heated and stirred under argon at 200° C. for 1.5 h or until a homogenous melt has formed. To the melt was added starting material (82 mg, 0.35 mmol). The reaction mixture was stirred at 200° C. for 2 h. The resultant solid black mass was cooled to 100° C. Methanol (5 mL) and water (10 mL) were added and the reaction mixture was sonicated until the black mass had become soluble. Dichloromethane (40 mL) was added and concentrated ammonia (˜2 mL) was added to adjust the mixture to pH=10. The organic layer was separated, and the aqueous layer was extracted with dichloromethane (3×20 mL). The combined organic layers were dried over MgSO4, filtered, and concentrated. Purification by preparative TLC plate (1:10 v/v methanol-dichloromethane) yielded 26 mg (0.08 mmol, 22% yield) of the title compound as a yellow solid. 1H-NMR (MeOH-d4) 8.37 (d, J=7.8 Hz, 3H), 7.86 (s, 1H), 7.55 to 7.77 (m, 5H), 7.27 to 7.33 (m, 4H), 4.31 (s, 2H); MS ES 346 (M+H)+; TLC (1:10 v/v methanol-dichloromethane) Rf=0.45.
The procedure used for the preparation of Example 4 was used to prepare the title compound by substituting 6-aminobenzothiazole for 4-chloroaniline in step 3. 1H-NMR (MeOH-d4) 9.08 (s, 1H), 8.37 to 8.59 (m, 4H), 7.79 to 8.01 (m, 2H), 7.60 to 7.78 (m, 4H), 7.30 (d, 2H), 4.34 (s, 2H); MS ES 369 (M+H)+; TLC (1:4 v/v hexane-ethyl acetate) Rf=0.20.
The procedure used for the preparation of Example 4 was used to prepare the title compound by substituting 5-aminoindane for 4-chloroaniline in step 3. 1H-NMR (MeOH-d4) 8.35 (m, 3H), 7.46 to 7.77 (m, 5H), 7.15 to 7.27 (m, 4H), 4.26 (s, 2H), 2.87 to 2.90 (m, 4H), 2.05 to 2.10 (m, 2H); MS ES 352 (M+H)+; TLC (1:4 v/v hexane-ethyl acetate) Rf=0.25.
The procedure used for the preparation of Example 4 was used to prepare the title compound by substituting 3-fluoro-4-methylaniline for 4-chloroaniline in step 3. 1H-NMR (MeOH-d4) 8.34 (d, 3H), 7.87 (s, 1H), 7.54 to 7.69 (m, 4H), 7.10 to 7.31 (m, 4H), 2.22 (s, 3H); MS ES 344 (M+2H)+; TLC (1:4 v/v hexane-ethyl acetate) Rf=0.20.
Step 1: A dry, 2 L, 3-necked, round-bottomed flask was equipped with a mechanical stirrer and addition funnel. To the flask was added 2-thiophenecarboxylic acid (25 g, 195 mmol) in anhydrous THF (500 mL) under argon. The mixture was cooled to −78° C. with a dry ice-isopropanol bath and allowed to stir for 30 min. n-Butyllithium in Hexanes (2.5 M, 172 mL) was added dropwise over 30 min. The reaction was kept at −78° C. for an additional hour with stirring then placed under an atmosphere of dry carbon dioxide. With addition of the carbon dioxide the reaction became thick. The reaction remained at −78° C. for an additional hour before warming to −10° C. The reaction was quenched with 2 N HCl (213 mL) and allowed to reach rt. The layers were separated and the aqueous layer was extracted with EtOAc (3×200 mL). The organic layers were combined, dried (Na2SO4) and concentrated by rotary evaporation. The brown solid was crystallized from hot isopropanol and dried overnight under vacuum. Desired thiophene-2,3-dicarboxylic acid was obtained (27.3 g, 159 mmol; 82% yield); 1H NMR (DMSO-d6) 7.69 (d, J=1.5, 1), 7.38 (d, J=4.8, 1); ES MS (M+H)+=173; TLC (Chloroform-MeOH-water, 6:4:1); Rf=0.74.
Step 1A: Alternatively, 3-thiophenecarboxylic acid rather than 2-thiophenecarboxylic acid has been used in step 1 to yield the same product.
Step 2: A 1 L, round-bottomed flask was equipped with a stir bar and reflux condenser. To the flask was added the product of step 1 (62 g, 360 mmol) in MeOH (500 mL) with a catalytic amount of H2SO4 (˜5 mL). The reaction was heated to reflux and stirred for 24 h. The reaction was cooled to rt and concentrated rotary evaporation. The brown mixture was purified by silica gel chromatography (Hexane-EtOAc 80:20 gradient to 60:40). Desired dimethyl thiophene-2,3-dicarboxylate was obtained (21.2 g, 106 mmol; 31% yield); 1H NMR (DMSO-d6) 7.93 (d, J=4.8, 1), 7.35 (d, J=4.8, 1), 3.8 (d, J=1, 6); ES MS (M+H)+=201; TLC (Hexane-EtOAc, 70:30); Rf=0.48.
Step 3: A 250 mL, round-bottomed flask was equipped with a stir bar and reflux condenser. To the flask was added the product of step 2 (16 g, 80 mmol), hydrazine hydrate (6.6 mL, 213 mmol), and EtOH (77 mL) and refluxed for 2.5 h. The reaction was cooled to rt and concentrated by rotary evaporation. Water (50 mL) was added and the filtrate was separated from the insoluble solids. The aqueous layer was concentrated by rotary evaporation to give a pale yellow solid. The solid was dried in a vacuum oven overnight at 50° C. Desired thieno[2,3-d]pyridazin-4,7-dione was obtained (12 g, 71 mmol; 89% yield); 1H NMR (DMSO-d6) 7.85 (d, J=5.1, 1), 7.42 (d, J=5.1, 1); ES MS (M+H)+=169; TLC (dichloromethane-MeOH, 60:40); Rf=0.58.
Step 4: Preparation of Intermediate B: A 250 mL, round-bottomed flask was equipped with a stir bar and reflux condenser. To the flask was added the product of step 3 (2.5 g, 14.8 mmol), phosphorus oxychloride (45 mL, 481 mmol), and pyridine (4.57 mL, 55 mmol) and refluxed for 2.5 h. The reaction was cooled to rt and poured over ice. The mixture was separated and the aqueous layer was extracted with chloroform (4×75 mL). The organic layers were combined, dried (Na2SO4) and concentrated by rotary evaporation to give a dark yellow solid. Desired 4,7-dichlorothieno[2,3-d]pyridazine (Intermediate B; 1.5 g, 7.3 mmol; 49% yield); mp=260-263° C.; 1H NMR (DMSO-d6) 8.55 (d, J=5.7, 1), 7.80 (d, J=5.7, 1); ES MS (M+H)+=206; TLC (hexane-EtOAc, 70:30); Rf=0.56. See also Robba, M.; Bull. Soc. Chim. Fr.; 1967, 4220-4235.
Step 5: A 250 mL, round-bottomed flask was equipped with a stir bar and reflux condenser. To the flask was added the product of step 4 (7.65 g, 37.3 mmol), 4-chloroaniline (4.76, 37.3 mmol) in EtOH (75 mL). The mixture was refluxed for 3 h. An orange solid precipitated from the reaction after 3 h. The reaction was cooled to rt and the solid was collected by filtration and washed with hexane. The desired 7-chloro-4-(4-chlorophenylamino)thieno[2,3-d]pyridazine was obtained (6.5 g, 21.9 mmol; 60% yield); mp=139-142° C.; ES MS (M+H)+=297; TLC (Hexane-EtOAc, 60:40); Rf=0.48.
Step 6: A 150 mL, round-bottomed flask was equipped with a stir bar and reflux condenser. To the flask was added the product of step 5 (0.33 g, 1.1 mmol), 4-pyridylcarbinol (1.2 g, 11.2 mmol) in DBU (2.5 mL, 16.7 mmol) and the mixture was heated to 125° C. for 24 hours. EtOAc (10 mL) was added to the reaction while hot and then the reaction was poured into water (10 mL). The layers were separated and the aqueous layer was extracted with EtOAc (3×10 mL). The organic layers were combined, dried (MgSO4) and concentrated by rotary evaporation. The resulting mixture was purified by silica gel chromatography (dichloromethane-methanol-acetone, 90:5:5) to give a pale yellow solid. The desired title compound was obtained (0.03 g, 0.08 mmol; 7.3% yield); mp=203-205° C. dec; ES MS (M+H)+=369; TLC (dichloromethane-methanol-acetone, 95:2.5:2.5); Rf=0.56.
Step 1: n-Butyllithium (2.5M in hexanes, 196 mL, 491 mmol) was introduced into a dry 3 L 3-necked flask fitted with an addition funnel, argon inlet, and mechanical stirrer. The mixture was diluted with dry THF (500 mL), and cooled to −78° C. 3-furoic acid (25 g, 223 mmol) was added as solution in THF (500 mL) dropwise. The mixture was stirred for 1.5 h, at which point dry carbon dioxide was bubbled through the reaction mixture for 1 h. After warming gradually to −10° C., the resultant thick white slurry was treated with aqueous HCl (2 N, 446 mL). The two layers were separated, and the aqueous layer was extracted with EtOAc (3×300 mL). The combined organics were dried (Na2SO4), filtered, and concentrated to afford crude furan-2,3-dicarboxylic acid as an orange solid (44 g) which was used without further purification. 1H NMR (300 MHz, d6-acetone) δ 7.06 (d, J=1.7, 1), 7.97 (d, J=1.7, 1), 10.7 (bs, 2H); TLC(CHCl3/MeOH/H2O 6:4:1) Rf=0.56.
Step 2: A dry 500 mL round bottomed flask was equipped with a stir bar and an argon inlet. The flask was charged with the crude diacid prepared in Step 1 (44 g) dissolved in MeOH (250 mL). To the reaction mixture was added chlorotrimethylsilane (80 mL, 630 mmol) portionwise. After stirring at room temperature for 15.5 h, the solution was concentrated to an oil and silica (5 g) was added. The mixture was suspended in MeOH (100 mL), and the volatiles were removed. Suspension in MeOH (100 mL) and the removal of the volatiles was repeated an additional two times. The residue was applied directly to the top of a flash chromatography column and was eluted hexanes/EtOAc 60:40 to yield dimethyl furan-2,3-dicarboxylate as an orange oil (38 g, 93% for Step 1 and Step 2 combined). 1H NMR (300 MHz, CDCl3) δ 3.81 (s, 3), 3.86 (s, 3), 6.71 (d, J=2.8, 1), 7.46 (d, J=2.8, 1); TLC (hexanes/EtOAc 60:40) Rf=0.46.
Step 3: A 500 mL round bottomed flask fitted with an argon inlet, a reflux condenser, and a stir bar was charged with dimethyl furan-2,3-dicarboxylate (44 g, 236 mmol) dissolved in EtOH (250 mL). Hydrazine hydrate (55% N2H4, 40 mL, 3.0 mmol) was added to the solution, and the reaction mixture was warmed to reflux. A yellow solid slowly precipitated over the course of 5.5 h, at which point the mixture was cooled to room temperature. The volatiles were removed under reduced pressure to furnish a yellow paste which was suspended in water and filtered. The yellow solid was washed with water and transferred to a 500 mL round bottomed flask fitted with an argon inlet, a reflux condenser, and a stir bar. The solid was suspended in aqueous HCl (2N, 200 mL), and the mixture was warmed to reflux. After heating for 4 h, the orange slurry was cooled to room temperature and filtered. The solid was washed thoroughly with water and dried under vacuum to yield 4,7-dioxo[2,3-d]furopyridazine as an orange solid (21.5 g, 60%). NMR (300 MHz, d6-DMSO) δ 7.00 (d, J=2.1, 1), 8.19 (d, J=2.1, 1H), 11.7 (bs, 2H).
Step 4: Preparation of Intermediate C: A 1 L round bottomed flask was fitted with a reflux condenser, a stir bar, and an argon inlet. The furan from Step 3 (15.5 g, 102 mmol) was added to a mixture of phosphorous oxychloride (300 mL) and pyridine (30 mL), and the resultant orange suspension was warmed to reflux. After heating the reaction mixture for 4 h, the volatiles were removed by rotary evaporation. The residue was poured onto ice, and the aqueous mixture was extracted with CHCl3 (4×250 mL). The combined organics were washed with brine, dried (MgSO4) and concentrated to afford 4,7-dichloro[2,3-d]furopyridazine (Intermediate C, 11.3 g, 59%) as an orange-red solid which was used without further purification. TLC (hexanes/EtOAc) Rf=0.352; 1H NMR (300 MHz, d6-DMSO) δ 7.40 (d, J=2.0, 1), 8.63 (d, J=2.0, 1).
Step 5: A 100 mL round bottomed flask fitted with a stir bar, an argon inlet, and a reflux condenser was charged with the product of Step 4 (1.50 g, 7.98 mmol) dissolved in ethanol (40 mL). Chloroaniline was added to this mixture (1.02 g, 7.98 mmol), and the resultant suspension was warmed to reflux. After heating for 4 h, the mixture was concentrated by rotary evaporation. The crude orange solid was applied to the top of a flash column and eluted with CH2Cl2/MeOH 97:3 to afford a mixture of 4-chloro-7-[N-(4-chlorophenyl)amino][2,3-c]furopyridazine and 7-chloro-4-[N-(4-chlorophenyl)amino]-[2,3-d]furopyridazine as a yellow powder (1.2 g, 55%). TLC (CH2Cl2/MeOH 97:3); Rf=0.7; NMR (300 MHz, d6-DMSO) δ major isomer (A) 7.40 (d, J=8.9, 2), 7.45 (d, J=2.0, 1), 7.87 (d, J=9.2, 2), 8.34 (d, J=2.0, 1) 9.62 (s, 1); minor isomer (B) 7.28 (d, J=2.0, 1), 7.40 (d, J=8.9, 2), 7.87 (d, J=9.2, 2), 8.48 (d, J=2.1, 1), 9.88 (s, 1).
Step 6: A 25 mL round bottomed flask was fitted with an argon inlet, a stir bar, and a reflux condenser. The product of step 5 (400 mg, 1.4 mmol) was combined with 4-pyridylcarbinol (782 mg, 7.17 mmol) and 1,8-diazabicyclo[5.4.0]undec-7-ene (2.5 mL 16.7 mmol), and the slurry was warmed to 125° C. After stirring for 24, the reaction was cooled, applied directly to the top of a flash column, and eluted with CH2Cl2/MeOH 95:5. The resultant yellow oil was rechromato-graphed under the same conditions to yield the title compound as part of a mixture of three components. HPLC separation (C18 column CH3CN/H2O 10:90 gradient to 100:0) furnished the title compound as an off white solid (13.7 mg, 3%). TLC (CH2Cl2/MeOH 95:5)=0.19; MP 198° C.; 1H NMR (300 MHz, CDCl3) δ 5.60 (s, 2), 6.6 (d, J=2.1, 1), 7.18-7.20 (m, 2), 7.35-7.43 (m, 6), 7.66 (d, J=2.1, 1) 8.54 (d, J=5.6, 2).
Steps 5A and 6A: Alternatively 4,7-dibromo[2,3-d]furopyridazine (Intermediate G below) is used to prepared the title compound by following step 5 but substituting the dibromo intermediate for the dichloro intermediate. Step 6A is conducted by melting the two components together in the presence of CsCO4 rather than 1,8-diazabicyclo[5.4.0]undec-7-ene. The crude product is purified as above.
Intermediates D to G: Preparation of other bicyclic 4,5-fused-3,6-dihalopyridazines
The general procedures of example 9, steps 2 to 4 are used by substituting the appropriate heterocycledicarboxylic acid for furan-2,3-dicarboxylic acid to yield the substituted dichloropyridazines D to G found in the below table. The dibromofuropyridazine G was prepared using steps 2-3 from example 9 and then conducting step 4′ as follows: to 0.50 g (3.287 mmol) of the product of step 3 was added 2.83 g (6.57 mmol) of phosphorus pentabromide. This was heated to 125° C. At about 115° C. the reaction mixture melted and then re-solidified before it reached 125° C. The reaction mixture was cooled and the solid residue was crushed up and dumped into ice water. The resulting solid was then filtered and vacuum dried. wt.=0.75 g (82% yield). In several cases the dichloropyridazines are known materials, as indicated by the given reference. All of these dihaloheterocycles can be used to prepare the claimed invention compounds.
Intermediate H: Preparation of (2-methylaminocarbonyl-4-pyridyl)methanol
Step 1: A stirred solution of ethyl isonicotinate (250 mL, 1.64 mole) and concentrated sulfuric acid (92 mL, 1.64 mole) in N-methylformamide (2.0 L) was cooled to 6° C. with an ice bath. Iron (II) sulfate heptahydrate (22.8 g, 0.0812 mole, milled with a mortar and pestle) was added, followed by the dropwise addition of 30% aqueous hydrogen peroxide (56 mL, 0.492 mole). The additions of iron (II) sulfate and hydrogen peroxide were repeated four additional times, while the reaction temperature was kept below 22° C. After the reaction mixture was stirred for thirty minutes, sodium citrate solution (2 L, 1 M) was added (pH of the resulting mixture was about 5). The mixture was extracted with dichloromethane (1 L, 2×500 mL). The combined organic extracts were washed with water (2×500 mL), 5% aqueous sodium bicarbonate (3×100 mL), and brine (500 mL). The resulting organic solution was then dried over sodium sulfate, filtered and concentrated in vacuo to afford a solid. The crude solid was triturated with hexanes, filtered, washed with hexanes and dried under vacuum to give 270.35 g (79.2%) of pastel yellow solid. 1H NMR (DMSO-d6, 300 MHz): S 8.9 (d, 1H), 8.3 (m, 1H), 8.0 (dd, 1H), 4.4 (q, 2H), 2.8 (d, 3H), 1.3 (t, 3H).
Step 2: To a mechanically stirred slurry of the product of step 1 (51.60 g, 0.248 mole) in EtOH (1.3 L) was added sodium borohydride (18.7 g, 0.495 mole). The reaction mixture was stirred at rt for 18 hr. The resulting solution was quenched carefully with saturated aqueous ammonium hydrochloride (2 L). Gas evolution was observed during quenching. The resulting mixture was basified with conc. ammonium hydroxide solution (200 ml) to pH=9. It was then extracted with EtOAc (8×400 mL). The combined organic layers were dried (MgSO4), filtered, and concentrated in vacuo to give Intermediate H as a clear light yellow oil (36.6 g, 89% yield). 1H NMR (DMSO-d6, 300 MHz): δ 8.74 (q, 1H), 8.53 (dd, 1H), 7.99 (m, 1H), 7.48 (m, 1H), 5.53 (t, 1H), 4.60 (d, 2H), 2.81 (d, 3H); MS m/z 167 [M+H]+.
Intermediates I to N: General Method for Preparation of [2-(N-Substituted)aminocarbonyl-4-pyridyl]methanol Intermediates
To a 0° C. solution of the amine 2 (3 equiv) in benzene is added trimethyl aluminum (3 equiv). Gas evolution is observed and the reaction is then allowed to warm to rt and stir for 1 h. (Lipton, M. F. et al. Org. Synth. Coll. Vol. 6, 1988, 492 or Levin, J. I. et al. Synth. Comm., 1982, 12, 989). The known carbinol 1 (1 equiv, Hadri, A. E.; Leclerc, G. Heterocyclic Chem, 1993, 30, 631) is added to the aluminum reagent and the mixture is heated to reflux for 1 h. The reaction is quenched with water and concentrated. The crude product is usually purified by silica gel column chromatography (20/1 EtOAc/MeOH) to afford title compound 3. The final products are generally confirmed by LC/MS and NMR spectroscopy.
A 25 mL, 3-necked, round-bottomed flask was equipped with a stir bar and thermometer. To the flask was added the product of Example 8 (0.475 g, 1.29 mmol), iron sulfate heptahydrate (0.179 g, 0.64 mmol), formamide (11.15 mL, 281 mmol) and conc. H2SO4 (0.14 mL). The mixture was stirred for 30 min at rt at which time H2O2 (0.2 mL, 6.44 mmol) was added drop wise to the mixture. The reaction stirred at room temperature for an additional hour and then heated to 55° C. over 30 min. The reaction was kept at this temperature for 3 h and then cooled to rt. An aqueous solution of sodium citrate (0.27M, 1 mL) was added to the reaction and subsequently the layers were separated and the aqueous layer was extracted with EtOAc (4×5 mL). The organic layers were combined, dried (MgSO4) and concentrated by rotary evaporation. The resulting solid was taken up in hot acetone and separated from any remaining solids by filtration. The filtrate was then concentrated by rotary evaporation and the resulting residue was taken up in hot MeOH and the white solid was collected by filtration. Desired compound (0.014 g, 0.034 mmol; 2.7% yield); mp=233° C.; ES MS (M+H)+=412; TLC (dichloromethane-methanol-acetone, 95:2.5:2.5); Rf=0.20.
The procedure used for the preparation of Example 10 was used to prepare the title compound by substituting methylformamide for formamide: 1H NMR (DMSO-d6) 8.80 (d, 1), 8.62 (d, 1), 8.31 (d, 1), 8.09 (d, 2), 7.86 (d, 2), 7.65 (d, 1), 7.35 (d, 2), 5.74 (s, 2), 2.84 (d, 3); ES MS (M+H)+=426 (ES); Rf (95/2.5/2.5 DCM/MeOH/Acetone)=0.469.
The procedure used for the preparation of Example 10 was used to prepare the title compound by substituting the product of example 4 for the product of example 8. The crude product was purified by preparative TLC plate (1:4 v/v hexane-ethyl acetate, 19% yield) of the title compound as a yellow solid. 1H-NMR (MeOH-d4) 8.42 (d, 1H), 8.34 (d, 1H), 7.94 (s, 1H), 7.88 (s, 1H), 7.55 to 7.76 (m, 5H), 7.26 to 7.36 (m, 3H), 4.34 (s, 2H); MS ES 389 (M+H)+; TLC (1:4 v/v hexane-ethyl acetate) Rf=0.44.
The procedure used for the preparation of Example 11 was used to prepare the title compound by substituting the product of example 4 for the product of Example 8. The crude product was purified by column chromatography (2:3 v/v hexane-ethyl acetate, 20% yield) of the title compound as a yellow solid. 1H-NMR (MeOH-d4) 8.42 (d, 1H), 8.33 (d, 1H), 7.88 (d, 2H), 7.55 to 7.77 (m, 5H), 7.28 to 7.36 (m, 3H), 4.34 (s, 2H), 2.89 (s, 3H); MS ES 403 (M+H)+; TLC (2:3 v/v hexane-ethyl acetate) Rf=0.30.
To a suspension of the final product from Example 9 (19.20 g, 54.4 mmol) in N-methylformamide (200 mL) and distilled water (20 mL) at room temperature was added concentrated H2SO4 (2.9 mL, 54.4 mmol) dropwise. The mixture was stirred until it became a clear solution. To this solution was added FeSO4.7H2O (1.51 g, 5.43 mmol) in one portion, followed by the addition of hydroxylamine-O-sulfonic acid (HOSA, 1.84 g, 16.3 mmol). The additions of FeSO4.7H2O and HOSA were repeated in 10 min. intervals for 11 times. HPLC assay showed the consumption of most starting material. The reaction mixture was cooled with an ice bath. A solution of sodium citrate (600 mL, 1M, 600 mmol) was added under vigorous stirring. The resulting suspension was stirred vigorously for additional 10 min. The solid was collected by filtration, washed with water (3×100 mL), and dried under vacuum at 50° C. for 16 hours. The crude product (21 g) was purified by filtering through a silica gel pad eluting with 5% CH3OH/CH2Cl2. The resulting 3.7 g product was recrystallized in CH3CN (125 mL, boiled for 1.5 hours). The solid was collected by filtration, washed with CH3CN (2×15 mL), and dried under vacuum at 50° C. for 16 hours. The final product (4-(4-chlorophenylamino)-7-(2-methylaminocarbonyl-4-pyridylmethoxy)furo-[2,3-d]pyridazine) is a light yellow solid (3.38 g, 15.2%). mp=223-224° C.
A major byproduct was isolated through the above silica gel pad filtration. The structure of the byproduct (4-(4-chlorophenylamino)-2-methylaminocarbonyl-7-(2-methylaminocarbonyl-4-pyridylmethoxy)furo-[2,3-d]pyridazine) was characterized by 1H NMR, 2D NMR, elemental analysis, and MS. 1H NMR (DMSO-d6, 300 MHz): δ 9.32 (br s, 1H), 8.93 (q, 1H), 8.79 (q, 1H), 8.63 (dd, 1H), 8.12 (m, 1H), 7.91 (m, 3H), 7.70 (dd, 1H), 7.35 (m, 2H), 5.76 (br s, 2H), 2.81 (d, 6H). MS m/z 467 [M+H]+.
To a mixture of the Intermediate from Example 9, step 5 (10.0 g, 35.7 mmol), Intermediate H (12.4 g, 74.6 mmol), and 18-crown-6 (0.42 g, 1.59 mmol) in toluene (100 mL) was added KOH powder (4.4 g, 85%, 66.7 mmol) in one portion at room temperature. The reaction mixture was then heated to 85±2° C. under vigorous stirring. The reaction mixture was stirred vigorously at this temperature overnight. After it was cooled to room temperature, toluene solution was decanted off and water (100 mL) was added to the gummy residue. The resulting mixture was stirred vigorously until it became a free flowing suspension. The solids were collected by filtration, washed with water (2×10 mL), and dried under vacuum at 45° C. for 16 hours. The yellow/brown solids were suspended in acetonitrile (70 mL) and the suspension was stirred at reflux for 2 hours. After it was cooled to room temperature, the solids were collected by filtration, washed with small amount of acetonitrile, and dried under vacuum at 45° C. overnight. The title product was isolated in 46% yield (6.73 g) as a light yellow solid.
The procedure used for the preparation of Example 14 was used to prepare the title compound by substituting Formamide for N-methylformamide. The reaction was conducted with 500 mg of final product from Example 9 and proportional amounts of solvents and reagents. The crude product was purified by HPLC on a 75×30 mm C18 column and a linear gradient elution from 10 to 100% acetonitrile in water with 0.1% trifluoroacetic acid at 10 ml/min. over 10 min. to yield 18 mg of the title compound as a yellow solid: HPLC (50×4.6 mm YMC CombiScreen® C18 column, linear gradient 10 to 100% acetonitrile in water with 0.1% trifluoroacetic acid at 3 ml/min. over 5 min., UV detection at 254 nm) 2.35 min. peak; MS ES 396 (M+H)+.
To the dichloride from Example 8, step 4 (1.00 g, 4.90 mmol) was added p-chloroaniline (622 mg, 4.90 mmol) and absolute ethyl alcohol (10.0 mL). The mixture was refluxed at 95° C. for 2 hrs and then cooled to room temperature. The yellow precipitate (2) that formed was filtered and washed with isopropyl alcohol, 4.0 N KOH, H2O, and then hexane. The filtrate (2) was then mixed 6-aminobenzothiazole (883 mg, 5.88 mmol) in 10 mL of n-butanol, and heated at 150° C. overnight. The reaction was allowed to cool to room temperature before the solvent was removed by rotary evaporation. The residue was treated sequentially with aqueous 4.0 N KOH solution and extracted with dichloromethane (50 mL), dried (MgSO4), and the solvent evaporated. The crude product was purified by flash chromatography on silica gel using 95% dichloromethane/methanol as the eluent. The structure of the pure title compound was confirmed by LC/MS and NMR: TLC (30% EtOAc/Hexanes) Rf(3)=0.20; 1H NMR (DMSO)S 7.2 (dd, 3H), 7.38 (dd, 3H), 7.65 (d, 1H), 8.0 (d, 1H), 8.45 (d, 1H), 8.8 (s, 1H); LC/MS m/z 410 rt=4.21 min.
The procedure used for the preparation of Example 17 was used to prepare the title compound by substituting 5-aminoindane for 4-chloroaniline. The crude product was purified by flash chromatography on silica gel using 30% ethyl acetate/hexane as the eluent. The structure of the pure title compound was confirmed by LC/MS and NMR: TLC (30% EtOAc/Hexanes) Rf(3)=0.20; (3) 1H NMR (DMSO) δ 2.0 (m, 2H), 2.85 (m, 4H), 7.18 (d, 1H), 7.8 (d, 1H), 7.95 (d, 1H), 8.10 (d, 1H), 8.18 (d, 1H), 8.7 (d, 2H), 9.1 (d, 2H), LC/MS m/z 414 rt=4.43 min.
4,7-Dichloro[2,3-d]furopyridazine from step 4 of Example 9 (95 mg, 0.50 mmol) and 5-bromoindoline (100 mg, 0.50 mmol) were refluxed in 60 mL of absolute ethanol at 95° C. for 2 hrs. The reaction mixture was allowed to cool to room temperature and the precipitate that formed was filtered and washed with isopropyl alcohol, 4.0 N KOH, H2O, and hexane, and then dried. The intermediate of about 95% purity (rt=4.72, (M+H)+350) and was used in the next step without further purification. 4-Pyridylcarbinol (28 mg, 0.26 mmol) and sodium hydride (60%, 50 mg, 1.25 mmol) were stirred in 20 mL of anhydrous tetrahydrofuran at 0° C. under Argon for 20 min. and then 44 mg of the above intermediate (0.13 mmol) was added. The reaction was stirred at 0° C. for 2 hrs and the temperature was allowed to rise to room temperature. The mixture was stirred for another 12 hrs and the solvent was evaporated under reduced pressure. The solid that was obtained was dissolved in 50 mL of dichloromethane and washed with K2CO3 solution and H2O. The organic layer was separated, dried (MgSO4), and evaporated under reduced pressure. The crude product was purified by preparative TLC(Rf=0.3) on silica gel using dichloromethane/methanol (95:5) as the eluent. The structure of the pure title compound was confirmed by LC/MS and NMR: 1H NMR (CDCl3) δ 3.20 (m, 2H), 4.30˜4.50 (m, 2H), 5.60 (s, 2H), 6.9˜8.0 (m, 7H), 8.60 (m, 2H); LC/MS (M+H)+423 rt=4.49 min.
To a suspension of 4,7-Dichloro[2,3-d]furopyridazine from step 4 of Example 9 (400 mg, 2.12 mmol, 1 equiv) and p-anisidine (p-MeOC6H4NH2) (260 mg, 2.12 mmol, 1 equiv) in DME (5 mL) was added water (1 mL). The resulting solution was heated at 50° C. for 48 h. After cooling to rt, the brown precipitate was removed by filtration and the filtrate was concentrated in vacuo to afford the crude product as a brown solid. Trituration of the brown solid with CH2Cl2 furnished 292 mg (50%) of the intermediate 4-(4-methoxyphenylamino)-7chlorofuro-[2,3-d]pyridazine which was confirmed by LC/MS and NMR. A suspension of this intermediate (292 mg, 1.06 mmol, 1 equiv), (2-methylaminocarbonyl-4-pyridyl)methanol (Intermediate H, 529 mg, 3.18 mmol, 3 equiv) and 18-crown-6 (42 mg, 0.16 mmol, 15 mol %) in toluene (4 mL) was stirred at rt for 20 min. KOH (178 mg, 3.18 mmol, 3 equiv) was then added and the reaction mixture was heated to 80° C. for 36 h. After cooling to rt, water (10 mL) was added and the mixture was stirred vigorously until a fine white suspension was formed. The suspension was filtered and washed with water and diethyl ether to provide 125 mg (29%) of the desired product as a light yellow solid: (M+H)+406; R1=0.50 (100% EtOAc).
The procedure used for the preparation of Example 20 was used to prepare the title compound by substituting 4-pyridylmethanol for (2-methylaminocarbonyl-4-pyridyl)methanol. The pure product was isolated by chromatography on a flash column: (M+H)+349; R1=0.3 (95:5 CH2Cl2/CH3OH).
The procedure used for the preparation of Example 16 was used to prepare the title compound by substituting the product of Example 21 for the product from Example 9. The reaction was conducted with 250 mg of the starting material and proportional amounts of solvents and reagents. The crude product was purified by HPLC on a 75×30 mm C18 column and a linear gradient elution from 10 to 100% acetonitrile in water with 0.1% trifluoroacetic acid at 10 ml/min. over 10 min. to yield 16 mg of the title compound as a yellow solid: HPLC (50×4.6 mm YMC CombiScreen® C18 column, linear gradient 10 to 100% acetonitrile in water with 0.1% trifluoroacetic acid at 3 ml/min. over 5 min., UV detection at 254 nm) 1.98 min. peak; MS ES 392 (M+H)+.
Method A-1: Equal equivalents of dichloride (1) and M-NH2 are refluxed in the appropriate amount of absolute ethanol at 95° C. for 2 hrs. The reaction mixture is allowed to cool to room temperature and the precipitate (2) that forms is filtered and washed sequentially with isopropyl alcohol, 4.0 N KOH, H2O, and hexane, and then dried. The filtrate (2) is then reacted with 1.2 equivalent of Q-NH2 in an appropriate amount of n-butyl alcohol at 150° C. for 10 hrs. The reaction is cooled to room temperature before the solvent is evaporated under reduced pressure. The residue is treated with aqueous 4.0 N KOH solution and extracted with dichloromethane. The organic layer is dried (MgSO4) and evaporated. The crude product (3) is purified by preparative thin layer chromatography (TLC) or flash chromatography on silica gel using dichloromethane/methanol (95:5) as the eluent. Final product is confirmed by LC/MS and/or NMR. The invention compounds of Examples 23-25, 48, and 76-80 as shown in the below table were prepared by method A-1.
Method A-2: One equivalent of dichloride (1) and 2.2 equivalent of M-NH2 are refluxed in an appropriate amount of n-butanol at 150° C. for 10 hrs. The reaction mixture is allowed to cool to room temperature and the precipitate (4) that forms is filtered and washed sequentially with isopropyl alcohol, 4.0 N KOH, H2O, and hexane, and then dried. The crude product (4) is purified by preparative TLC or flash chromatography on silica gel using dichloromethane/methanol (95:5) as the eluent. Final product is confirmed by LC/MS and/or NMR. The invention compounds of Examples 26-33 and 75 as shown in the below table were prepared by method A-2.
Method A-3: One equivalent of dichloride (1) and one equivalent of M-NH2 are suspended in DME (0.3M) and water is added until a solution was formed. The reaction mixture is heated to 65° C. for 48 h. After cooling to rt, the resulting precipitate is filtered and washed with DME to provide the intermediate product (2) which is confirmed by LC/MS and NMR. In some instances, intermediate (2) is further purified by preparative TLC or washed with other solvents. A suspension of (2) (1 equiv), carbinol (3) (3 equiv), and 18-crown-6 (10 mol %) in toluene (0.3M) is stirred at rt for 10 min. KOH (3 equiv) is then added and the reaction mixture is heated to 80° C. for 24 h. After cooling to rt, water is added and the mixture is stirred vigorously until a suspension is formed. The suspension is filtered and washed with water to provide the desired product (4). Preparative TLC and/or washing with other solvents is used to further purify final products in some examples. The final products are assigned by LC/MS and NMR spectroscopy. Final product is confirmed by LC/MS and/or NMR. The invention compounds of Examples 34-47, 49-74, and 81-82D as shown in the below table were prepared by method A-3.
Compounds that were Prepared by Parallel Methods A-1, A-2 or A-3
A-1d
63c
A-3e
A-3e
A-3f
aHPLC - electrospray mass spectra (HPLC ES-MS) were obtained using a Hewlett-Packard 1100 HPLC equipped with a quaternary pump, a variable wavelength detector, a YMC Pro C18 2.0 mm x 23 mm column, and a Finnigan LCQ ion trap mass spectrometer with electrospray ionization. Gradient elution from 90% A to 95% B over 4 minutes was used on the HPLC. Buffer A was 98% water, 2% Acetonitrile and 0.02% TFA. Buffer B was 98% Acetonitrile, 2% water and 0.018% TFA. Spectra were scanned from 140-1200 amu using a variable ion time according to the number of ions in the source;
bAn HPLC assay with UV peak detection was run in addition to the HPLC ES-MS experiment and the conditions are: 50 x 4.6 mm YMC CombiScreen ® C18 column, linear gradient 10 to 100% acetonitrile in water with 0.1% trifluoroacetic acid at 3 ml/min. over 5 min., UV detection at 254 nm;
cThe product was purified by RP-HPLC on a C18 column using a water/acetonitrile gradient with added trifluoroacetic acid such that the trifluoroacetate salt was isolated by evaporation of the pure product;
d4-pyridylmethanol as indicated, was used in step 2 of method A-1 rather than an amine;
eFor preparation of 5-amino-2,3-dihydrobenzofurane see Mitchell, H.; Leblanc, Y. J. Org. Chem. 1994, 59, 682-687.
fThe reference to make the known TBS protected alcohol intermediate is: Parsons, A. F.; Pettifer, R. M. J. Chem. Soc. Perkin Trans. 1, 1998, 651.
The deprotection of
was accomplished in the following manner: Three equiv of a 1.0 Molar solution of TBAF in THF was added to a solution of the protected alcohol in THF (0.05 Molar) at rt. The reaction mixture was allowed to stir at rt for 1 h and was quenched with water followed by extraction with EtOAc.
Method B-1: Dibromoisoquinoline (5, 29 mg, 0.1 mmol) Example 1, step 1, and M-NH2 (0.2 mmol) in 8-mL vial were heated in 1 mL of n-butanol at 90° C. for 36 hrs. The mixture was cooled to room temperature and the solvent was evaporated under reduced pressure. 4-Mercaptopyridine (23 mg, 0.2 mmol) and cesium carbonate (67 mg, 0.2 mmol) were added to the vial. The mixture was heated at 180° C. for 1 hr and was allowed to cool to room temperature. Methanol (2 mL) was added to the vial and the mixture was sonicated for 10 min and filtered. The methanol solution of reaction mixture was collected and evaporated under reduced pressure. The formation of product was confirmed by LC/MS. The invention compounds of Examples 83-92 as shown in the below table were prepared by method B-1.
Compounds that were Prepared by Method B-1
Method A-1 or A-2, as indicated, were used to prepare the novel phthalimide invention compounds 93-105 from 1,4-dichlorophthalazine (for preparation see Novartis patent WO98/35958, Nov. 2, 1998) rather than the dichloroheterocyclopyridazines together with the appropriate bicyclic and substituted anilines.
Novel Phthalazines that were Prepared by Methods A-1 or A-2
The product of Example 14 (1.50 g, 3.66 mmol) was stirred as a slurry in methanol (20 ml) as a solution of toluenesulfonic acid hydrate (0.701 g, 3.67 mmol) in methanol (5 ml plus 5 ml rinse) was added quickly dropwise. All materials dissolved over 5 min to yield a yellow solution. Anhydrous ether (30 ml) was added and stirring was continued for 5 minutes until solid began to precipitate. The resultant mixture was chilled with stirring in an ice/water bath for 45 minutes and then the solid title product (Example 104) was collected by filtration, washed with ether and dried at 55° C. in a vacuum oven until NMR analysis showed a lack of solvents (1.5 hours). Other compounds were prepared in a similar way by using a variety of acids rather than toluenesulfonic acid. Scale up and use of less methanol in the first step generally led to quicker precipitation of salts and a variety of solvents were used rather than ether, as indicated, to help crystallize the individual salts. In some cases the methanol was first removed by evaporation in vacuo. Final drying took between 1.5 hours and several days, depending on the quantity of material and the specific specific acid used.
Salts of Example 14 that were Prepared
Biological Protocols and In Vitro Test Data
The effects of the compounds and drug combinations according to the invention are tested in vitro on isolated rat pulmonary arteries and in vivo in monocrotaline-treated rats with pulmonary hypertension.
Isolated Small Pulmonary Arteries
Male Wistar rats (250-300 g) are anaesthetized with ether, and the lungs are removed. The left pulmonary arterial vessel is dissected and placed in ice-cold Krebs-Henseleit (KH) buffer of following composition (in mmol/l): NaCl 112, KCl 5.9, CaCl2 2.0 MgCl2 1.2, NaH2PO4 1.2, NaHCO3 25, glucose 11.5 and optionally the compound/combination to be tested in a concentration of 10−10 to 10−4 mol/1.
For measurement of isometric tension, ring segments, 2 mm in length, are mounted in a small vessel chamber myograph. Two wires (40 μm diameter) are introduced through the lumen of the segments and mounted according to the method described by Mulvany and Halpern (Circulation Research 1977; 41:19-26). After a 30 min equilibration period in oxygenated KH solution at 37° C. and pH=7.4, segments are stretched to their optimal lumen diameter for active tension development which is determined based on the internal circumference-wall tension ratio of the segments by setting their internal circumference to 90% of what the vessels would have if they are exposed to a passive tension equivalent to that produced by a transmural pressure of 30 mmHg.
Afterwards, segments are washed three times with KH solution and left to equilibrate for 30 min. Segment contractility is then tested by an initial exposure to a high K+ solution (120 mmol/l K+—KH solution, which is identical to KH solution except that NaCl is replaced by KCl on an equimolar basis).
The vessels are than pre-contracted using K+ (50 mmol/l) KH solution. When the contraction is stabilized, an accumulative dose response curve of the compound/combination tested is constructed. The stabilized contraction induced by K+ (50 mmol/l) KH solution is defined as 100% tension. The relaxation is expressed as percentage tension.
Pulmonary Artery Pressure in Monocrotaline Treated Rats
Male Sprague Dawley rats (250-300 g) are treated with monocrotaline 60 mg/kg subcutanously (=day 0). On day 14 after monocrotaline injection treatment the compound/combination to be tested is administered. On day 28 hemodynamic parameters, i.e. right ventricular pressure, systemic blood pressure, heart rate, arterial and venous oxygen saturation are measured and compared with untreated control animals.
Results:
The mentioned monocrotaline (MCT) treated rats are randomized to receive example 14 [4-(4-chlorophenylamino)-7-(2-methylaminocarbonyl-4-pyridylmethoxy)furo-[2,3-d]pyridazine] 10 mg/kg or vehicle by gavage once daily after the onset of moderate pulmonary arterial hypertension starting 14 days after the injection of MCT until the final hemodynamic measurement on day 28. In animals with MCT induced pulmonary arterial hypertension treatment with example 14 markedly decreases right ventricular systolic pressure, compared to vehicle treated animals (control: 25±0.56 mmHg; example 14: 42.04±3.21 mmHg vs. placebo: 71.02±5.38 mmHg) (mean±SEM). This effect of example 14 is paralleled by an inhibition of right ventricular hypertrophy (right ventricle/left ventricle+septum ratio control: 0.26±0.01; example 14: 0.32±0.02 vs. placebo: 0.54±0.04).
| Number | Date | Country | Kind |
|---|---|---|---|
| 06007929 | Apr 2006 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP2007/002985 | 4/3/2007 | WO | 00 | 12/28/2009 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2007/118602 | 10/25/2007 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 20060041006 | Ibrahim et al. | Feb 2006 | A1 |
| Number | Date | Country |
|---|---|---|
| 103 10 908 | Sep 2004 | DE |
| WO-00-09495 | Feb 2000 | WO |
| WO-01-23375 | Apr 2001 | WO |
| WO-2006-066937 | Jun 2006 | WO |
| WO-2007-039736 | Apr 2007 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20100113452 A1 | May 2010 | US |
