Patent Application
20070191457
This invention relates to novel compounds having pharmacological activity, processes for their preparation, to compositions containing them and to their use in the treatment of CNS and other disorders.
WO 96/23783, WO 97/46699 and WO 97/48700 all disclose a series of indoline derivatives which are 5-HT2C receptor antagonists and which are claimed to be useful in the treatment of various CNS disorders.
A novel class of compounds possessing 5HT2C receptor activity has been found. The present invention therefore provides, in a first aspect, a compound of formula (I) or a pharmaceutically acceptable salt thereof:
wherein:
R1 is halogen, cyano, C1-6alkyl, C3-7cycloalkyl, C3-7cycloalkyloxy, C1-6alkoxy, C1-6alkylthio, hydroxy, amino, mono- or di-C1-6alkylamino, an N-linked 4 to 7 membered heterocyclic group, nitro, haloC1-6alkyl, haloC1-6alkoxy, aryl, —COOR3, —COR4 (wherein R3 and R4, are independently hydrogen or C1-6alkyl) or —COR5 (wherein R5 is amino, mono- or di-C1-6alkylamino or an N-linked 4 to 7 membered heterocyclic group);
p is 0, 1 or 2 or 3;
Q is a 6-membered aromatic group or a 6-membered heteroaromatic group;
A is —(CH2—CH2)—, —(CH═CH)—, or a group —(CHR7)— wherein R7 is hydrogen, halogen, hydroxy, cyano, nitro, C1-6alkyl, C3-7cycloalkyl, C3-7cycloalkyloxy, haloC1-6alkyl, C1-6alkoxy, haloC1-6alkoxy or C1-6alkylthio;
R2 is hydrogen, halogen, hydroxy, cyano, nitro, C1-6alkyl, C1-6alkanoyl, C3-7cycloalkyl, C3-7cycloalkyloxy, haloC1-6alkyl, C1-6alkoxy, haloC1-6alkoxy, C1-6alkylthio, amino, mono- or di-C1-6alkylamino or an N-linked 4 to 7 membered heterocyclic group;
X is oxygen, sulfur, —CH2— or NR8 wherein R8 is hydrogen or C1-6alkyl;
Y is a single bond, —CH2—, —(CH2)2— or —CH═CH—; and
Z is an optionally substituted N-linked heterocyclic group or a C-linked 4 to 7 membered heterocyclic group containing at least one nitrogen, or Z is —NR9R10 where R9 and R10 are independently hydrogen or C1-6alkyl.
The following terms, whether used alone or as part of another group are to be given the following meanings, unless otherwise stated.
The term “halogen” and its abbreviated form “halo” are used herein to describe fluorine, chlorine, bromine or iodine.
The term “alkyl” is used herein to describe a straight chain or branched fully saturated hydrocarbon group. “C1-6alkyl” refers to alkyl groups having from one to six carbon atoms, including all isomeric forms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, neopentyl, sec-pentyl, n-pentyl, isopentyl, tert-pentyl and hexyl.
The term “C1-6alkanoyl” refers to an alkanoyl group having from 1 to 6 carbon atoms, such as methanoyl (or “formyl”), ethanoyl (or “acetyl”), propanoyl, isopropanoyl, butanoyl, isobutanoyl, sec-butanoyl, pentanoyl, neopentanoyl, sec-pentanoyl, isopentanoyl, tertpentanoyl and hexanoyl.
The term “C1-6alkoxy” refers to a straight chain or branched chain alkoxy (or “alkyloxy”) group having from one to six carbon atoms, including all isomeric forms, such as methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentoxy, neopentoxy, sec-pentoxy, n-pentoxy, isopentoxy, tert-pentoxy and hexoxy.
The term “C3-7cycloalkyl” refers to a cycloalkyl group consisting of from 3 to 7 carbon atoms, such as cyclopropane, cyclobutane, cyclopentane, cyclohexane and cycloheptane. Optional substituents for C3-7cycloalkyl includes one or more halogen, hydroxy, oxo, C1-6alkyl, cyano, CF3, OCF3, C1-6alkoxy and C1-6alkanoyl.
The term “C1-6alkylthio” refers to a straight chain or branched chain alkylthio group having from one to six carbon atoms, such as methylthio, ethylthio, propylthio, isopropylthio, butylthio, isobutylthio, sec-butylthio, tert-butylthio, pentylthio, neopentylthio, sec-pentylthio, n-pentylthio, isopentylthio, tert-pentylthio and hexylthio.
The term “mono- or di-C1-6alkylamino” refers to an amino group which is substituted by one C1-6alkyl group or an amino group which is substituted by two C1-6alkyl groups, the two amino groups being the same or different. Examples of monoC1-6alkylamino include methylamine, ethylamine, propylamine, isopropylamine, butylamine, isobutylamine, sec-butylamine, tert-butylamine, pentylamine, neopentylamine, sec-pentylamine, n-pentylamine, isopentylamine, tert-pentylamine and hexylamine. Examples of di-C1-6alkylamino include dimethylamine, diethylamine, dipropylamine, diisopropylamine, dibutylamine, diisobutylamine, disec-butylamine, ditert-butylamine, dipentylamine, dineopentylamine, dihexylamine, butylmethylamino, isopropylmethylamino, ethylisopropylamino, ethylmethylamino, etc.
The term “5- or 6-membered aromatic or heteroaromatic group” is used herein to describe groups such as phenyl, pyrrolyl, pyrrolinyl, pyrazolinyl, imidazolyl, pyrazolyl, oxadiazolyl, isothiazolyl, thiazolyl, pyridyl, thiazinyl, pyridazinyl, pyrimidinyl and pyrazinyl.
The term “aryl” is used herein to describe 5- or 6-membered aromatic or heteroaromatic groups such as phenyl, pyrrolyl, pyrrolinyl, pyrazolinyl, imidazolyl, pyrazolyl, oxadiazolyl, isothiazolyl, thiazolyl, pyridyl, thiazinyl, pyridazinyl, pyrimidinyl and pyrazinyl, as well as naphthyl and other bicyclic heteroaromatic groups. These groups may be optionally substituted by one or more of C1-6alkyl (to form “arylC1-6alkyl”), halogen, CF3 or C1-6alkoxy (to form “arylC1-6alkoxy”).
The terms “halo C1-6alkoxy” or “haloC1-6alkyl” are used to describe a C1-6alkoxy or a C1-6alkyl group, respectively, substituted with one or more halogens. Examples include —CHCl2, —CF3, —OCF3, etc.
The term “heterocyclic group” is used herein to describe a stable aromatic or non-aromatic ring containing 1, 2 or 3 heteroatoms selected from nitrogen, sulphur and oxygen. Suitable examples of 4 to 7 membered heterocyclic groups include azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, oxazolinyl, isothiazolidinyl, thiazolidinyl, pyrrolyl, pyrrolinyl, pyrazolinyl, imidazolyl, pyrazolyl, isothiazolyl, thiazolyl, piperidyl, piperazinyl, morpholinyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, azepinyl, azepanyl, dioxolanyl, thienyl, tetrahydrothienyl, tetrahydrofuryl, dioxanyl and dithianyl.
The term “N-linked heterocyclic group” is used herein to describe a heterocyclic group which is linked to the remainder of the molecule via a nitrogen atom. Suitable examples of 4 to 7 membered N-linked heterocyclic groups include azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, oxazolinyl, isothiazolidinyl, thiazolidinyl, pyrrolyl, pyrrolinyl, pyrazolinyl, imidazolyl, pyrazolyl, piperidyl, piperazinyl, morpholinyl and azepanyl.
The term “C-linked heterocyclic group containing at least one nitrogen” is used herein to describe a heterocyclic group which is contains at least one nitrogen atom and is linked to the remainder of the molecule via a carbon atom. Suitable examples of 4 to 7 membered C-linked heterocyclic groups containing at least one nitrogen include azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, pyrrolyl, pyrrolinyl, pyrazolinyl, imidazolyl, pyrazolyl, piperidyl, piperazinyl, morpholinyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, azepinyl and azepanyl.
The term “optionally substituted N-linked or C-linked 4 to 7 membered heterocyclic group” means that more than one optional substituent may be present in the N-linked or C-linked 4 to 7 membered heterocyclic group, which may be the same or different, and may be attached to any carbon atom of the heterocycle or an available nitrogen atom.
Suitable optional substituents for the N-linked or C-linked heterocycle include C1-6alkyl, amino, mono- or di-C1-6alkylamino, aryl, arylC1-16alkyl, arylamino, hydroxy, C1-6alkylamido, hydroxyC1-6alkyl, C1-6alkoxycarbonyl, halogen, haloC1-6alkyl, a heteroaromatic group (such as indole or benzimidazole), an aromatic or non-aromatic N-linked or C-linked heterocycle or an aromatic or non-aromatic heterocycleC1-6alkyl optionally substituted by C1-6alkyl. Examples of aromatic or non-aromatic heterocycleC1-6alkyl include heterocycle-methyl (such as pyridinyl-methyl and benzimidazolyl-methyl) and heterocycle-ethyl (such as morpholinyl-ethyl and indolyl-ethyl).
Substituents in the N-linked or C-linked heterocycle may form a bridge structure, to form a group such as for example 2-oxa-5-azabicyclo[2.2.1]heptyl. Such a bicyclic group may be further substituted by the substituents listed above. More than one substituent may be present on the same carbon atom to form spiro structures such as 1,4 and 1,5 dioxa spiro compounds.
When p is 2 or 3, R1 may be the same or different.
When A is a group —(CHR7)—, preferably R7 is hydrogen. Preferably A is CH2—.
Preferably Q is phenyl, as follows:
Preferably p is 1, 2 or 3 and R1 is/are halogen (particularly chloro or fluoro), C1-6alkyl (particularly methyl) or CF3.
If R1 is attached at the position marked below with an asterisk, then R1 is preferably fluoro:
When Q is phenyl and p is 1, preferably R1 is attached at the position marked below with an asterisk:
When Q is phenyl and p is 2 or 3, preferably R1 is attached at two or more of the positions marked below with arrows:
Preferably R2 is C1-6alkoxy (particularly methoxy).
Preferably X is oxygen.
Preferably Y is —CH2—.
Preferably Z is an optionally substituted N-linked 4 to 7 membered heterocycle, in particular optionally substituted piperidyl. Preferred substituents include halogen (particularly fluoro) and C1-6alkyl (particularly methyl).
Preferred compounds are compounds of formula (Ia):
wherein R1, p, R2, X, Y, Z, are as defined for formula (I) and A1 is —CH2— or —HC(Me)—. Preferred features of formula (I) also apply to formula (Ia).
Preferred compounds include:
The compounds of formula (I) can form acid addition salts. It will be appreciated that for use in medicine the salts of the compounds of formula (I) should be pharmaceutically acceptable. Suitable pharmaceutically acceptable salts will be apparent to those skilled in the art and include those described in J. Pharm. Sci., 1977, 66, 1-19, such as acid addition salts formed with inorganic acids e.g. hydrochloric, hydrobromic, sulfuric, nitric or phosphoric acid; and organic acids e.g. succinic, maleic, acetic, fumaric, citric, tartaric, benzoic, p-toluenesulfonic, methanesulfonic or naphthalenesulfonic acid.
The compounds of this invention may be in crystalline or non-crystalline form, and, if crystalline, may optionally be hydrated or solvated. This invention includes within its scope stoichiometric hydrates as well as compounds containing variable amounts of water.
Certain compounds of formula (I) are capable of existing in stereoisomeric forms (e.g. geometric or (“cis-trans”) isomers, diastereomers and enantiomers) and the invention extends to each of these stereoisomeric forms and to mixtures thereof including racemates. The different stereoisomeric forms may be separated one from the other by the usual methods, or any given isomer may be obtained by stereospecific or asymmetric synthesis. The invention also extends to any tautomeric forms and mixtures thereof.
The present invention also provides a process for the preparation of a compound of formula (I) or a pharmaceutically acceptable salt thereof, which process comprises:
(a) reacting a compound of formula (II):
wherein R1, R2, p, A, X, and Y are as defined for formula (I), and L is a leaving group, with a compound of formula (III):
Z-H (III)
wherein Z is as defined for formula (I); or
(b) reacting a compound of formula (IV):
wherein Rx is alkyl and LG is a suitable leaving group, with a compound of formula (V) or a corresponding salt;
or
(c) reacting a compound of formula (VI):
with a compound of formula (V) in the presence of AlMe3 or a similar oxophilic reagent followed by treatment of the resulting amide under dehydrating conditions, e.g. with PPh3 and dialkylazadicarboxylate;
and thereafter, for either process (a), process (b) or process (c), optionally followed by:
For the reaction of process (a), suitably L is mesylate. The reaction may take place in a solvent such as DMF in the presence of sodium iodide and potassium carbonate.
For the reaction of process (b), LG is a leaving group such as halogen, for example chloro or bromo, or a sulfonate ester, for example mesyl or tosyl. Rx is alkyl, for example Me. The reaction may take place in a solvent such as DMF at elevated temperature, suitably 150° C. in a microwave reactor. Where this reaction does not lead to spontaneous cyclisation of the intermediate alkylation product this material is treated with AlMe3 or a similar oxophilic reagent.
Compounds of formula (I) can be converted into further compounds of formula (I) using standard techniques. For example, and by way of illustration rather than limitation, a compound wherein A is —(HCOH)— may be converted to a compound wherein A is —(CH2)— by using a suitable reducing agent such as triethylsilane-trifluoroacetic acid using dichloromethane as solvent.
Compounds of formulae (II), (III) and (IV) are commercially available or may be prepared according to methods described herein or may be prepared according to known methods or by analogous methods thereto.
Those skilled in the art will appreciate that it may be necessary to protect certain groups to carry out the above processes. Suitable protecting groups and methods for their attachment and removal are conventional in the art of organic chemistry, such as those described in Greene T. W. ‘Protective groups in organic synthesis’ New York, Wiley (1981).
Pharmaceutically acceptable salts may be prepared conventionally by reaction with the appropriate acid or acid derivative.
In another aspect, the present invention provides a pharmaceutical composition comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier or excipient.
In a further aspect, the present invention provides a process for preparing a pharmaceutical composition, the process comprising mixing a compound of formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier or excipient.
A pharmaceutical composition of the invention, which may be prepared by admixture, suitably at ambient temperature and atmospheric pressure, is usually adapted for oral, parenteral or rectal administration and, as such, may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, reconstitutable powders, injectable or infusible solutions or suspensions or suppositories. Orally administrable compositions are generally preferred.
Tablets and capsules for oral administration may be in unit dose form, and may contain conventional excipients, such as binding agents (e.g. pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose), fillers (e.g. lactose, microcrystalline cellulose or calcium hydrogen phosphate), tabletting lubricants (e.g. magnesium stearate, talc or silica), disintegrants (e.g. potato starch or sodium starch glycollate); and acceptable wetting agents (e.g. sodium lauryl sulphate). The tablets may be coated according to methods well known in normal pharmaceutical practice.
Oral liquid preparations may be in the form of, for example, aqueous or oily suspension, solutions, emulsions, syrups or elixirs, or may be in the form of a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents (e.g. sorbitol syrup, cellulose derivatives or hydrogenated edible fats), emulsifying agents (e.g. lecithin or acacia), non-aqueous vehicles (which may include edible oils e.g. almond oil, oily esters, ethyl alcohol or fractionated vegetable oils), preservatives (e.g. methyl or propyl-p-hydroxybenzoates or sorbic acid), and, if desired, conventional flavourings or colorants, buffer salts and sweetening agents as appropriate. Preparations for oral administration may be suitably formulated to give controlled release of the active compound.
For parenteral administration, fluid unit dosage forms are prepared utilising a compound of the invention or pharmaceutically acceptable salt thereof and a sterile vehicle. Formulations for injection may be presented in unit dosage form e.g. in ampoules or in multi-dose, utilising a compound of the invention or pharmaceutically acceptable salt thereof and a sterile vehicle, optionally with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile pyrogen-free water, before use. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions, the compound can be dissolved for injection and filter sterilised before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents are dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner, except that the compound is suspended in the vehicle instead of being dissolved, and sterilisation cannot be accomplished by filtration. The compound can be sterilised by exposure to ethylene oxide before suspension in a sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound.
Lotions may be formulated with an aqueous or oily base and will in general also contain one or more emulsifying agents, stabilising agents, dispersing agents, suspending agents, thickening agents, or colouring agents. Drops may be formulated with an aqueous or non-aqueous base also comprising one or more dispersing agents, stabilising agents, solubilising agents or suspending agents. They may also contain a preservative.
The compounds of the invention may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. containing conventional suppository bases such as cocoa butter or other glycerides.
The compounds of the invention may also be formulated as depot preparations. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds of the invention may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
For intranasal administration, the compounds of the invention may be formulated as solutions for administration via a suitable metered or unitary dose device or alternatively as a powder mix with a suitable carrier for administration using a suitable delivery device. Thus compounds of formula (I) may be formulated for oral, buccal, parenteral, topical (including ophthalmic and nasal), depot or rectal administration or in a form suitable for administration by inhalation or insufflation (either through the mouth or nose).
The compounds of the invention may be formulated for topical administration in the form of ointments, creams, gels, lotions, pessaries, aerosols or drops (e.g. eye, ear or nose drops). Ointments and creams may, for example, be formulated with an aqueous or oily base with the addition of suitable thickening and/or gelling agents. Ointments for administration to the eye may be manufactured in a sterile manner using sterilised components.
The compounds of the present invention have affinity for the 5-HT2C receptor. The affinity can be determined by assessing their ability to displace [3H]-mesulergine from rat or human 5-HT2C clones expressed in 293 cells in vitro, as described in WO 94/04533.
All the Example compounds were tested according to this assay and were found to have pKi values >5.8. Some compounds show a considerably higher affinity in the range of 7.0 to >9.0 in human cells.
The intrinsic activity of the compounds of this invention can be determined according to the [35S]GTPγS functional assay which is described in WO 99/07700.
Compounds of formula (I) and their pharmaceutically acceptable salts are of use in the treatment of certain CNS disorders such as depression (which term is used herein to include bipolar depression, unipolar depression, single or recurrent major depressive episodes with or without psychotic features, catatonic features, melancholic features, atypical features or postpartum onset, seasonal affective disorder, dysthymic disorders with early or late onset and with or without atypical features, neurotic depression and social phobia, depression accompanying dementia for example of the Alzheimer's type, vascular dementia with depressed mood, schizoaffective disorder or the depressed type, and depressive disorders resulting from general medical conditions including, but not limited to, myocardial infarction, diabetes, miscarriage or abortion, etc), anxiety including generalised anxiety and social anxiety disorder, schizophrenia, panic disorder, agoraphobia, social phobia, epilepsy, obsessive compulsive disorder and post-traumatic stress disorder, pain (particularly neuropathic pain), migraine, memory disorders, including dementia, amnesic disorders and age-associated memory impairment, disorders of eating behaviours including anorexia nervosa and bulimia nervosa, sexual dysfunction, sleep disorders (including disturbances of circadian rhythm, dyssomnia, insomnia, sleep apnea and narcolepsy), withdrawal from abuse of drugs such as of cocaine, ethanol, nicotine, benzodiazepines, alcohol, caffeine, phencyclidine (phencyclidine-like compounds), opiates (e.g. cannabis, heroin, morphine), sedative ipnotic, amphetamine or amphetamine-related drugs (e.g. dextroamphetamine, methylamphetamine) or a combination thereof, Alzheimer's disease, motor disorders such as Parkinson's disease, dementia in Parkinson's disease, neuroleptic-induced Parkinsonism and tardive dyskinesias, as well as other psychiatric disorders, disorders associated with spinal trauma and/or head injury such as hydrocephalus, gastrointestinal disorders such as IBS (Irritable Bowel Syndrome), Crohn's disease, ulcerative colitis, non-steroidal anti-inflammatory drug induced damage) as well as microvascular diseases such as macular oedema and retinopathy.
It is to be understood that, as used herein, the term “treatment” refers to alleviation of established symptoms as well as prophylaxis.
Thus the present invention also provides a compound of formula (I) or a pharmaceutically acceptable salt thereof, for use as a therapeutic substance. In particular, the present invention provides a compound of formula (I) or a pharmaceutically acceptable salt thereof for use in the treatment of the above disorders. In particular the invention provides a compound of formula (I) or a pharmaceutically acceptable salt thereof for use as a therapeutic substance in the treatment of a CNS disorder. Preferably the CNS disorder is depression or anxiety.
Compounds of the invention may be administered in combination with other active substances such as 5HT3 antagonists, NK-1 antagonists, serotonin agonists, selective serotonin reuptake inhibitors (SSRI), noradrenaline re-uptake inhibitors (SNRI), tricyclic antidepressants or dopaminergic antidepressants.
Suitable 5HT3 antagonists which may be used in combination of the compounds of the inventions include for example ondansetron, granisetron, metoclopramide.
Suitable serotonin agonists which may be used in combination with the compounds of the invention include sumatriptan, rauwolscine, yohimbine, metoclopramide.
Suitable SSRIs which may be used in combination with the compounds of the invention include fluoxetine, citalopram, femoxetine, fluvoxamine, paroxetine, indalpine, sertraline, zimeldine.
Suitable SNRIs which may be used in combination with the compounds of the invention include venlafaxine and reboxetine.
Suitable tricyclic antidepressants which may be used in combination with a compound of the invention include imipramine, amitriptiline, chlomipramine and nortriptiline.
Suitable dopaminergic antidepressants which may be used in combination with a compound of the invention include bupropion and amineptine.
It will be appreciated that the compounds of the combination or composition may be administered simultaneously (either in the same or different pharmaceutical formulations), separately or sequentially.
The invention further provides a method of treatment of the above disorders in mammals including humans, which comprises administering to the sufferer a therapeutically safe and effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof. In particular the invention provides a method of treatment of a CNS disorder in mammals including humans, which comprises administering to the sufferer a therapeutically safe and effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof. Preferably the disorder is depression or anxiety.
In another aspect, the invention provides for the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for use in the treatment of the above disorders. In particular the present invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for use in the treatment of a CNS disorder. Preferably the CNS disorder is depression or anxiety.
The composition of the present invention may contain from 0.1% to 99% by weight, preferably from 10 to 60% by weight, of the active material, depending on the method of administration. The dose of the compound used in the treatment of the aforementioned disorders will vary in the usual way with the seriousness of the disorders, the weight of the sufferer, and other similar factors. However, as a general guide suitable unit doses may be 0.05 to 1000 mg, more suitably 1.0 to 200 mg, and such unit doses may be administered more than once a day, for example two or three times a day. Such therapy may extend for a number of weeks or months. When administered in accordance with the invention, no unacceptable toxicological effects are expected with the compounds of the invention.
All publications, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein as though fully set forth.
The following Descriptions and Examples illustrate the preparation of compounds of the present invention.
General Procedure 1 (G1): Hydrochloride Salt Formation
To a solution of the free base in CH2Cl2 was added excess HCl (1M in Et2O). The resulting mixture was evaporated to dryness and the residue triturated using a mixture of EtOAc:Et2O ca. 1:1 and dried to give the respective hydrochloride salt.
General Procedure 2 (G2): Bromination to Form Substituted Benzyl Bromides
A mixture of the substituted toluene, N-bromosuccinimide (typically 1.1 eq.) and benzoyl peroxide (0.05 eq.) in dry CCl4 (2 ml per mmol substituted toluene) was heated at reflux.
After complete conversion (typically 5 h; NMR control) the mixture was allowed to cool, filtered (washing with Et2O), concentrated and submitted to column chromatography to give the respective substituted benzyl bromide.
General Procedure 3 (G3): Methyl Ester Formation
To the substituted benzoic acid in dry CH2Cl2 (ca. 0.2-0.5 M) containing catalytic DMF (ca. 0.1 eq.) at 0° C. was added with stirring oxalyl chloride (1.3 eq.). The ice bath was removed. After gas evolution had ceased (typically 1-2 h) volatiles were removed in vacuo. To the residue was added dry methanol (to give a ca. 1 M solution; CAUTION: on scales above ca. 5 mmol this involved a significant exotherme) and the mixture was kept for 16 h. The material obtained after evaporation of the volatiles was used as such or purified by column chromatography.
General Procedure 4 (G4): Chlorination to Form Substituted Benzyl Chlorides
A mixture of the substituted toluene, N-chlorosuccinimide (typically 1.1 eq.) and benzoyl peroxide (0.05 eq.) in dry CCl4 (2 ml per mmol substituted toluene) was heated at reflux. After complete conversion (typically 5 h; NMR control) the mixture was allowed to cool, filtered (washing with Et2O), concentrated and submitted to column chromatography to give the respective substituted benzyl chloride.
To a solution of 2-methoxy-5-nitrophenol (10 g) in DMF (45 ml) at room temperature were added K2CO3 (23 g) and 1-(2-chloroethyl)piperidin hydrochloride (12 g). The suspension was stirred at room temperature for 3 days. The suspension was diluted with water and twice extracted with EtOAc: Et2O 1:2, the combined organic phases were washed with brine, concentrated to dryness in vacuo, to obtain the title product as a brown oil (99%). NMR (1H, CDCl3): δ 7.85 (dd, 1H), 7.75 (d, 1H), 6.90 (d, 1H), 4.20 (t, 2H), 3.90 (s, 3H), 2.80 (t, 2H), 2.45-2.60 (m, 4H), 1.55-1.65 (m, 4H) (m, 4H), 1.35-1.50 (m, 2H). MS (m/z): 281[MH]+.
To 1-[2-(2-methoxy-5-nitro-phenoxy)-ethyl]-piperidine (59 mmol) in a mixture of MeOH (140 ml), NH4Cl (17.1 g), water (140 ml) and conc. aqueous HCl (4.9 ml) was added iron powder (10.6 g). The mixture was heated at reflux with vigorous stirring for 90 min. and concentrated to ca. half volume in vacuo. EtOAc was added, filtered (Celite), washing with water. Et2O was added, the layers were mixed and the aqueous layer collected. K2CO3 was added until pH ca. 9-10 and the mixture was twice extracted with EtOAc:Et2O 1:1. The combined organic layers were dried (K2CO3), filtered and concentrated to give the free base of the title compound as a red oil (56 mmol). NMR (1H, CDCl3): δ 6.67 (d, 1H), 6.30 (d, 1H), 6.20 (dd, 1H), 4.07 (t, 2H), 3.75 (s, 3H), 3.40 (bs, 2H), 2.80 (t, 2H), 2.40-2.55 (m, 4H), 1.51-1.62 (m, 4H), 1.35-1.46 (m, 2H).
Batches of this material were converted to the hydrochloride salt as required by treating a solution of the free base in dichloromethane with 1 eq. HCl (1 M solution in Et2O), followed by evaporation to dryness.
To a NaOH solution (14 g of NaOH, 350 mmol, in 300 ml of water) at 0° C. containing 4-metylpiperidine (36 ml, 310 mmol) chloroacetylchloride (23.7 ml, 305 mmol) was added in 10 min under vigorous stirring at 0° C. After 3 h the mixture was acidified using HCl (2M aqueous) and the aqueous phase was extracted twice with CH2Cl2 (2×150 ml). The combined organic extracts were dried over anhydrous Na2SO4, filtered and concentrated to dryness in vacuo to give 37 g of intermediate which was dissolved in N-methyl pyrrolidone (120 ml).
To this solution 2-methoxy-5-nitrophenol (24 g, 156 mmol), K2CO3 (21 g, 152 mmol) and NaI (2 g, 13 mmol) were added. The suspension was mechanically stirred and heated at 120° C. After 16 h the reaction was cooled and diluted with Et2O (500 ml), the organic phase was washed twice with water (2×150 ml), then with NaOH (aqueous, 1M, 100 ml) and finally with brine (150 ml).
The organic phase was dried over anhydrous Na2SO4, filtered and concentrated to dryness in vacuo to give 35 g of the title compound as a yellow solid. NMR (1H, CDCl3): δ 8.00 (dd, 1H), 7.76 (d, 1H), 6.98 (d, 1H), 4.87 (s, 2H), 4.55 (d, 1H), 4.02 (s, 3H), 3.85 (d, 1H), 3.13 (t, 1H), 2.68 (t, 1H), 1.55-1.85 (m, 4H), 1.10-1.30 (m, 1H), 1.01 (d, 3H). MS (m/z): 309 [MH]+.
2-(2-Methoxy-5-nitro-phenoxy)-1-(4-methyl-piperidin-1-yl)-ethanone (11 g, 35.7 mmol) was dissolved in anh. THF (20 ml), 1M BH3. THF (2.2 eq, 78.5 ml) was added dropwise to the solution, and the mixture was heated at reflux for 4 hours. The solution was cooled to room temperature, CH3OH (100 ml) was added, and the solvent was removed under reduced pressure. The residue was dissolved in CH3OH (100 ml), 6N HCl (200 ml) was added, and the solution was heated to reflux for 30′. The CH3OH was removed under reduced pressure and the remaining aqueous solution was made basic (pH>10) with 2.5 M NaOH. The basic solution was extracted with ethyl acetate (3×80 ml). The combined organic extracts were dried over anhydrous Na2SO4 and the solvent was removed under reduced pressure to give 9.4 g of the title product as a red oil. (yield: 90%). NMR (1H, CDCl3): δ 7.91 (dd, 1H), 7.80 (d, 1H), 6.90 (d, 1H), 4.21 (t, 2H), 3.96 (s, 3H), 2.97 (m, 2H), 2.85 (t, 2H), 2.12 (m, 2H), 1.64 (m, 2H), 1.4 (m, 1H), 1.29 (m, 2H), 0.92 (d, 3H). MS (m/z): 295 [MH]+.
To a solution of 1-[2-(2-methoxy-5-nitro-phenoxy)-ethyl]-4-methyl-piperidine (9.5 g, 32.3 mmol) in abs. EtOH (200 ml) were added Pd/C 10% (1 g) and the reaction mixture was hydrogenated at room temperature at 1 atm. After 5 hrs the reaction mixture was filtered on a Celite pad and the solution was concentrated in vacuo to give 8.5 g of the title product as a brown solid (yield: 99%). NMR (1H, CDCl3): δ 6.61 (d, 1H), 6.26 (d, 1H), 6.04 (dd, 1H), 4.60 (bs, 2H), 3.91 (t, 2H), 3.58 (s, 3H), 2.85 (m, 2H), 2.61 (t, 2H), 1.97 (m, 2H), 1.52 (m, 2H), 1.3 (m, 1H), 1.1 (m, 2H), 0.86 (d, 3H).
Batches of this material were converted to the hydrochloride salt as required as described for Preparation 2.
The title compound was obtained from 2-bromo-6-methyl-benzoic acid according to Procedure G3 (quant. yield): NMR (1H, CDCl3): δ 7.47 (t, 1H), 7.19-7.23 (m, 2H), 4.03 (s, 3H), 2.40 (s, 3H).
The compounds from Preparations 7 and 8 were prepared by applying Procedure G2 to the respective substituted toluenes:
To 2-chlorobenzoic acid (0.32 g) in dry THF (10 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.3 M in cyclohexane, 3.7 ml). After 1 h at −78° C. acetaldehyde (0.17 ml) was added in one portion with vigorous stirring. The mixture was allowed to warm to 0° C. before conc. aqueous HCl (0.4 ml) was added. The mixture was concentrated in vacuo, partitioned between water and CH2Cl2, the organic layer concentrated and submitted to column chromatography to yield the title compound as a colourless waxy solid (0.11 g). NMR (1H, CDCl3): δ 7.55 (t, 1H), 7.43 (d, 1H), 7.30 (d, 1H), 5.47 (q, 1H), 1.60 (d, 3H). MS (m/z): 183 [MH]+ (1Cl).
To a solution of 3,4-difluorophthalic anhydride (200 mg, 1.09 mmol) in dry MeOH (2 ml) at 0° C. was added MeONa (30% in MeOH, 1.14 mmol). The solution was allowed to warm to room temperature and stirred for 3 hours. After removing the solvent under reduced pressure, the residue was diluted with water, acidified using conc. aqueous HCl (1.09 mmol) and extracted with EtOAc (×3). The organic phases were collected, dried over Na2SO4, filtered and concentrated to dryness in vacuo to give the title compound (186 mg). NMR (1H, d6-DMSO): δ 13.6 (bs, 1H), 7.88/7.75 (m, 2H), 3.80 (s, 3H). MS (m/z): 215 [M−H]−.
3,4-Difluoro-phthalic acid-2-methyl ester (185 mg, 0.86 mmol), triethylamine (0.86 mmol) and ethyl chloroformate (0.86 mmol) were dissolved in 2 ml of dry THF under stirring at 0° C. The mixture was stirred for an additional 30 minutes at 0° C. then the precipitate was filtered off and washed with THF. The filtrate was added dropwise to a solution of NaBH4 (2.14 mmol) in H2O (1 ml) at 0° C. When the addition was completed the mixture was stirred at room temperature for 1 hour. The mixture was acidified with 1N HCl and concentrated under reduced pressure and the residue was extracted with CH2Cl2. The extract was dried over Na2SO4 and evaporated to give the title compound (100 mg). NMR (1H, d6-DMSO): δ 7.97 (dd, 1H), 7.80 (dd, 1H), 5.37 (s, 2H).
To a solution of 4,5-dichlorophthalic anhydride (5.0 g, 23 mmol) in dry MeOH (60 ml) at 0° C. was added MeONa (30% in MeOH, 24.2 mmol). The solution was allowed to warm to room temperature and stirred for 3 hours. After removing the solvent under reduced pressure, the residue was diluted with water, acidified using conc. aqueous HCl (23 mmol) and extracted with EtOAc (×3). The organic phases were collected, dried over Na2SO4, filtered and concentrated to dryness in vacuo to give the title compound as a colourless solid (5.3 g): NMR (1H, d6-DMSO): δ 13.7 (bs, 1H), 8.00 (s, 1H), 7.95 (s, 1H), 3.80 (s, 3H). MS (m/z): 247 [M−H]− (2Cl).
To 4,5-dichlorophthalic acid-2-methyl ester (22.7 mmol) and triethylamine (3.5 ml) in dry THF (75 ml) was added over 10 min via syringe at 0° C. with vigorous stirring ethyl chloroformate (2.4 ml). The mixture was stirred for an additional 30 minutes at 0° C. The precipitate was removed by filtration and washed with THF (10 ml). The filtrate was added over 15 min dropwise with stirring to a solution of NaBH4 (2.2 g) in H2O:THF 1:1 (75 ml) at 0° C. The mixture was stirred at room temperature for 1 hour, acidified (1M aqueous HCl, 100 ml) and partially concentrated under reduced pressure (to remove the bulk THF). The product was collected by filtration, washed with water and a small volume of MeOH (2 ml). The material thus obtained was partitioned between sat. aqueous NaHCO3 and CH2Cl2, dried (MgSO4) and evaporated to give the title compound (3.2 g) as a colourless solid: NMR (1H, d6-DMSO): δ 8.12 (s, 1H), 8.00 (s, 1H), 5.37 (s, 2H).
To 2,4-dichlorobenzoic acid (2.76 g) in dry THF (100 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.4 M in cyclohexane, 23.7 ml). After 1 h at −78° C. methyl Iodide (1.17 ml) was added in one portion with vigorous stirring. The mixture was allowed to warm to 25° C. over 4 h and water was added. After 5 min it was partitioned between aqueous HCl (1M) and EtOAc. The organic layer was washed (brine), dried (MgSO4), filtered and concentrated in vacuo to give a yellow oil (3 g) that was treated as described in procedure G3 (assuming 14.4 mmol substituted benzoic acid content). Column chromatography gave a yellow oil (3.0 g) which consisted of a mixture of compounds as judged by NMR. This material (using 3 g N-bromosuccinimide) was treated as described in procedure G2 to give, besides 3-bromomethyl-2,4-dichloro benzoic acid methyl ester (1.1 g) a colourless oil (1.9 g) consisting of a mixture of compounds as judged by NMR, containing 2-bromomethyl-4,6-dichlorobenzoic acid methyl ester. This material (estimated from NMR to contain 2.9 mmol substituted benzyl bromide) and 5-amino-2-methoxy phenol (0.48 g) in dry DMF (2 ml) were heated in a microwave reactor to 150° C. for 12 min. The mixture was concentrated in vacuo, triturated with CH2Cl2:EtOAc:petroleum ether 1:1:5 (ca. 15 ml), and then ca. 2 ml each of CH2Cl2 and MeOH to give the title compound as an off-white solid (0.61 g). Additional material 0.06 g) was obtained from chromatography of the organic extracts: NMR (1H, d6-DMSO): δ 9.16 (s, 1H), 7.76-83 (m, 2H), 7.37 (s, 1H), 7.12 (d, 1H), 6.95 (d, 1H), 4.88 (s, 2H), 3.75 (s, 3H). MS (m/z): 322 [M−H]− (2Cl).
3-Bromomethyl-2,4-dichloro benzoic acid methyl ester (1.1 g, cf synthesis in P14) was stirred for 1 h at 25° C. with Zn powder (0.5 g) in HOAc (5 ml). Et2O (20 ml) was added, the mixture filtered, washing with Et2O. The solution was concentrated in vacuo and the residue submitted to column chromatography to give the product as a slowly solidifying colourless oil (0.54 g): NMR (1H, CDCl3): δ 7.50 (d, 1H), 7.31 (d, 1H), 3.90 (s, 3H), 2.50 (s, 3H).
2,4-Dichloro-3-methyl benzoic acid methyl ester was heated at 45° C. for 1 h in MeOH (5 ml), THF (5 ml) and aqueous KOH (2 M, 2.5 ml). Water was added, the mixture concentrated somewhat in vacuo, acidified (aqueous HCl 1 M) and extracted with EtOAc. The organic layer was washed (brine), dried (MgSO4), concentrated and triturated with Et2O: petroleum ether 1:1 to give the title compound as a colourless solid (0.43 g): NMR (1H, CDCl3): δ 7.70 (d, 1H), 7.35 (d, 1H), 2.52 (s, 3H) (carboxylic acid proton not observed). MS (m/z): 203 [M−H]− (2Cl).
To 2,4-dichloro-3-methyl benzoic acid (0.43 g) in dry THF (10 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.4 M in cyclohexane, 3.5 ml). After 1 h formaldehyde (generated by thermal depolymerisation of 0.4 g paraformaldehyde and transferred via a nitrogen stream through a cannula) was blown onto the reaction mixture with vigorous stirring. The mixture was allowed to warm to 0° C. over 90 min and conc. aqueous HCl (1 ml) was added. After 1 h at 25° C. the mixture was concentrated in vacuo and submitted to column chromatography to give the title compound as a colourless creamy solid (0.14 g): NMR (1H, CDCl3): δ 7.41 (s, 1H), 5.18 (s, 2H), 2.54 (s, 3H).
5,6-Dichlorophthalide (P13, 1.5 g) was heated at 50° C. for 2 h in MeOH (15 ml), THF (15 ml) and aqueous KOH (2 M, 7.5 ml). Aqueous HCl (1 eq., 2 M) was added, the mixture concentrated to dryness in vacuo, and triturated with THF and subsequently with iPrOH to give a colourless solid (2 g). To this material in DMF (dry, 10 ml) and DCM (dry, 10 ml) was added imidazole (0.75 g) and tert-butyldimethylsilyl chloride (TBDMS-Cl, 1.7 g). After 16 h at 25° C. excess water was added, the mixture concentrated somewhat, acidified (1 M aqueous HCl) and extracted with Et2O. The organic layer was washed (water, 2*brine), dried (MgSO4), concentrated, triturated with some petroleum ether and submitted to column chromatography to give the title compound as a colourless solid (0.29 g, additional product contained in the petroleum ether solution from the trituration): NMR (1H, CDCl3): δ 8.13 (s, 1H), 7.87 (s, 1H), 5.00 (s, 2H), 0.94 (s, 9H), 0.12 (s, 6H) (carboxylic acid proton not observed). MS (m/z): 333 [M−H]− (2Cl).
To 2-(tert-butyl-dimethyl-silanyloxymethyl)-4,5-dichloro-benzoic acid (0.28 g) in dry THF (5 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.4 M in cyclohexane, 1.4 ml). After 1 h at −78° C. methyl iodide (0.078 ml) was added in one portion with vigorous stirring. The mixture was allowed to warm to 25° C. over 4 h and water and excess conc. aqueous HCl were added. The mixture was concentrated in vacuo and treated with excess HCl in Et2O and AcOEt for 1 h. The mixture was concentrated in vacuo and submitted to column chromatography to give the title compound as an orange solid (0.13 g): NMR (1H, CDCl3): δ 7.42 (s, 1H), 5.18 (s, 2H), 2.80 (s, 3H).
To a solution of 2-methoxy-5-nitro-phenol (1.0 g, 5.9 mmol) in dry DMF (20 ml), under N2, was added K2CO3 (1.1 eq., 0.9 g) and 2-bromoethyl acetate (1.1 eq, 0.714 ml). After heating to 60° C. for 4 hours, a saturated solution of NH4Cl was added and the mixture was extracted with dichloromethane. The organic phase was washed with water, dried over Na2SO4, filtered and then concentrated in vacuo, to give 1.5 g of the crude intermediate compound. This was dissolved in dry methanol (34 ml) and MeONa (31 mg, 0.58 mmol) was added. After 1 h an excess of Amberlite IR120 (H+ form) was added, the solution was filtered and concentrated in vacuo. The residue was dissolved in dichloromethane and washed with NaOH 0.1 N and water. The organic phase was dried over Na2SO4, filtered and then concentrated in vacuo, to give 1.1 g of the title compound as a pale yellow solid (m.p. 115° C.). NMR (1H, CDCl3): δ 7.95 (dd, 1H), 7.80 (d, 1H), 6.94 (d, 1H), 4.21 (t, 2H), 4.03 (t, 2H), 3.98 (s, 3H), 2.3 (bs, 1H). MS (m/z): 214 [MH]+.
Crude 2-(2-methoxy-5-nitro-phenoxy)-ethanol (0.43 g, 2 mmol) was dissolved in dry THF, 4,4-dimethylglutarimide (0.28 g, 2 mmol), triphenylphosphine (0.52 g, 2 mmol) and diethylazodicarboxylate (1.1 ml, 2.4 mmol) were added at 45° C. After 6 h the reaction mixture was concentrated in vacuo and purified by flash-chromatography (cyclohexane/ethyl ether 3/7) to give 0.41 g of the title compound as a white solid. NMR (1H, CDCl3): δ 7.88 (d, 1H), 7.73 (s, 1H), 6.85 (d, 1H), 4.30-4.15 (m, 4H), 3.90 (s, 3H), 2.52 (s, 4H), 1.13 (s, 6H).
1-[2-(2-Methoxy-5-nitrophenoxy)ethyl]-4,4-dimethylpiperidine-2,6-dione (426 mg, 1.26 mmol) was dissolved in dry THF (13 ml), 1M BH3. THF (2.2 eq, 3.8 ml) was added dropwise to the solution, and the mixture was heated at reflux for 4 hours. The solution was cooled to room temperature, CH3OH (10 ml) was added, and the solvent was removed under reduced pressure. The residue was dissolved in CH3OH (10 ml), 6N HCl (10 ml) was added, and the solution was heated to reflux for 2 h. The CH3OH was removed under reduced pressure and the remaining aqueous solution was made basic (pH>10) with 2.5 M NaOH. The basic solution was extracted with ethyl acetate (3×20 ml). The combined organic extracts were dried over anhydrous Na2SO4 and the solvent was removed under reduced pressure to give after flash chromatography (cyclohexane/ethyl acetate 3/7) the title compound (240 mg) as a yellow oil (62%): NMR (1H, CDCl3): δ 7.85 (d, 1H), 7.75 (s, 1H), 6.85 (d, 1H), 4.17 (t, 2H), 3.92 (s, 3H), 2.84 (t, 2H), 2.46 (m, 4H), 1.37 (m, 4H), 0.86 (s, 6H). MS (m/z): 309 [MH]+.
1-[2-(2-Methoxy-5-nitro-phenoxy)ethyl]-4,4-dimethylpiperidine (337 mg) was dissolved in MeOH (12 ml), Pd/C 10% was added and the mixture was allowed to react under H2 at atmospheric pressure for 3 h. The mixture was filtered and concentrated in vacuo to give 328 mg of the title compound as a pink oil: NMR (1H, CDCl3): δ 6.67 (d, 1H), 6.30 (s, 1H), 6.22 (d, 1H), 4.12 (t, 2H), 3.76 (s, 3H), 2.78 (t, 2H), 2.46 (m, 4H), 1.37 (m, 4H), 0.86 (s, 6H) (NH2 not observed). MS (m/z): 279 [MH]+.
2-Methoxy-4-nitro-phenol (338 mg) was allowed to react with 2-(hexamethylene-imino)ethyl chloride hydrochloride following Preparation 20 to give 417 mg of the title compound as a red oil: NMR (1H, CDCl3): δ 7.92 (dd, 1H), 7.75 (d, 1H), 6.87 (d, 1H), 4.28 (t, 2H), 3.95 (s, 3H), 3.05 (t, 2H), 2.78 (m, 4H), 1.80-1.45 (m, 8H). MS (m/z): 295 [MH]+.
1-[2-(2-Methoxy-5-nitrophenoxy)ethyl]azepane (324 mg) was reduced following Preparation 23 to give the title compound (230 mg) as a red oil: NMR (1H, CDCl3): δ 6.68 (d, 1H), 6.35 (d, 1H), 6.20 (d, 1H), 4.15 (t, 2H), 3.75 (s, 3H), 3.4-3.6 (m, 2H), 2.98 (t, 2H), 2.80 (m, 4H), 1.78-1.42 (m, 8H). MS (m/z): 265 [MH]+.
Benzyl 2-(chloromethyl)benzoate [obtained from the reaction of 2-(chloromethyl)benzoyl chloride (e.g. Hinton, I. G., Mann, F. G. J. Chem. Soc. 1959, 599-610) with benzyl alcohol] and 4-methoxy-3-(2-piperidin-1-yl-ethoxy)-phenylamine hydrochloride (P2, 1 eq.) in dry DMF (0.5 ml) were heated to 120° C. for 1 h. The mixture was concentrated in vacuo and submitted to column chromatography, followed by conversion to the hydrochloride salt (G1) to give the title compound as an off-white waxy solid (30 mg): NMR (1H, d6-DMSO): δ 10 (t, 1H), 7.75-7.77 (m, 2H), 7.65-7.69 (m, 2H), 7.53-7.56 (m, 1H), 7.37 (dd, 1H), 7.10 (d, 1H), 5.00 (s, 2H), 4.39 (t, 2H), 3.80 (s, 3H), 3.57 (bd, 2H), 3.48-3.51 (m, 2H), 3.00-3.06 (m, 2H), 1.68-1.84 (m, 5H), 1.37-1.41 (m, 1H).
MS (m/z): 367 [MH]+.
2-Bromomethyl-5-fluoro-benzoic acid methyl ester (P7, 0.12 g) and 4-methoxy-3-(2-piperidin-1-yl-ethoxy)-phenylamine hydrochloride (P2, 1.05 eq.) in dry DMF (0.5 ml) were heated to 120° C. for 2 h. The mixture was concentrated in vacuo, the residues partitioned between aqueous NaHCO3 and EtOAc, the organic layer concentrated and submitted to column chromatography, followed by conversion to the hydrochloride salt (G1) to give the title compound as an off-white solid (0.18 g): NMR (1H, CDCl3): δ 7.42-7.62 (m, 3H), 7.36 (d, 1H), 7.25-7.30 (m, 1H), 6.90 (d, 1H), 4.82 (s, 2H), 4.61 (bs, 2H), 3.85 (s, 3H), 3.61-3.74 (m, 2H), 3.37-3.47 (m, 2H), 2.83-2.97 (m, 2H), 2.18-2.38 (m, 2H), 1.80-1.95 (m, 3H), 1.35-1.51 (m, 1H) (hydrochloride proton not observed). MS (m/z): 385 [MH]+.
2-Bromo-6-bromomethyl-benzoic acid methyl ester (P8, 0.71 mmol) and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 1.5 eq.) in dry DMF (2 ml) were heated in a microwave reactor to 150° C. for 10 min. The mixture was concentrated in vacuo, and the residue submitted to column chromatography, followed by conversion to the hydrochloride salt (G1) to give the title compound as a colourless powder: NMR (1H, d4-MeOH): δ 7.79 (d, 1H), 7.69 (d, 1H), 7.61 (d, 1H), 7.52 (t, 1H), 7.26 (dd, 1H), 7.12 (d, 1H), 4.91 (s, 2H), 4.41 (t, 2H), 3.90 (s, 3H), 3.73-3.79 (m, 2H), 3.58 (bs, 2H), 3.11 (bt, 2H), 1.93-2.02 (m, 2H), 1.66-1.82 (m, 1H), 1.43-1.58 (m, 2H), 1.04 (d, 3H) (only data for major protonation isomer given). MS (m/z): 459/461 [MH]+ (1 Br).
To 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine (P5, 0.47 mmol) in dry toluene (1 ml) was added trimethylaluminium (2 M solution in hexanes, 1.1 ml). After 15 min a solution of 7-chloro-3-methyl-3H-isobenzofuran-1-one (P9) in dry CH2Cl2 (5 ml) was added. After 20 h the mixture was heated at 37° C. for 5 h. After cooling silica gel and CH2Cl2 were added and the material filtered and washed with a 1:10:30 mixture of conc. aqueous NH3:MeOH:CH2Cl2. Column chromatography of the resulting solution after concentration gave a brown film (0.12 g) which consisted of a mixture of compounds as judged by NMR. To this material was added PPh3 (0.26 g) and THF (dry, 1 ml) followed by slow addition over 20 min at 50° C. of diisopropyl azodicarboxylate (0.20 ml). After 30 min volatiles were evaporated and the residue submitted to repeated column chromatography followed by salt formation (G1) to give the title compound as a colourless film (12 mg). NMR (1H, d4-MeOH): δ 7.57-7.65 (m, 2H), 7.50 (dd, 1H), 7.36 (d, 1H), 7.12-7.14 (m, 2H), 5.28 (q, 1H), 4.40 (q, 2H), 3.92 (s, 3H), 3.75 (d, 2H), 3.55 (t, 2H), 3.10 (t, 2H), 1.98 (d, 2H), 1.74 (m, 1H), 1.50 (d, 2H), 1.40 (d, 3H), 1.04 (d, 3H)(only data for major protonation isomer given). MS (m/z): 429 [MH]+ (1Cl).
To 2-trifluoromethylbenzoic acid (0.95 g) in dry THF (30 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.3 M in cyclohexane, 9.2 ml). After 1 h at −78° C. methyl iodide (0.75 ml) was added in one portion with vigorous stirring. The mixture was allowed to slowly warm to 25° C. and kept for 16 h before sat. aqueous NaHCO3 was added. The mixture was stirred for 3 h, mixed with EtOAc:petroleum ether 1:1 and the layers were separated. The aqueous layer was acidified using 2 M aqueous HCl and extracted with EtOAc. This organic layer was collected, concentrated in vacuo and treated as described in procedure G3. Column chromatography gave a colourless oil (0.83 g) which consisted of a mixture of compounds as judged by NMR. This material (assuming 2 mmol content of the substituted toluene) was treated as described in procedure G2 to give material (0.73 g) consisting of a mixture of compounds as judged by NMR. This material and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 2.3 mmol) in dry DMF (4.5 ml) were heated in a microwave reactor to 150° C. for 15 min. The mixture was concentrated in vacuo, and the residue submitted to column chromatography, followed by conversion to the hydrochloride salt (G1) to give the title compound as a faint yellow solid: NMR (1H, d4-MeOH): δ 7.89 (t, 1H), 7.85-7.80 (m, 3H), 7.26 (dd, 1H), 7.12 (d, 1H), 5.01 (s, 2H), 4.42 (t, 2H), 3.90 (s, 3H), 3.76 (d, 2H), 3.58 (t, 2H), 3.12 (t, 2H), 1.98 (d, 2H), 1.74 (m, 1H), 1.50 (q, 2H), 1.04 (d, 3H)(only data for major protonation isomer given). MS (m/z): 449 [MH]+.
To 2,4-dichlorobenzoic acid (0.96 g) in dry THF (30 ml) was added slowly via syringe with vigorous stirring at −78° C. sec-butyllithium (1.3 M in cyclohexane, 9.2 ml). After 1 h at −78° C. methyl iodide (0.44 ml) was added in one portion with vigorous stirring. The mixture was allowed to warm to 25° C. over 4 h and water was added. After 5 min it was partitioned between aqueous HCl (1M) and EtOAc. The organic layer was washed (brine), dried (MgSO4), filtered and concentrated in vacuo to give a yellow oil that was treated as described in procedure G3 (assuming 5 mmol substituted benzoic acid content). Column chromatography gave a yellow oil (0.81 g) which consisted of a mixture of compounds as judged by NMR. This material (assuming 3.7 mmol content of the of substituted toluene) was treated as described in procedure G2 to give a colourless oil (0.59 g) consisting of a mixture of compounds as judged by NMR. Half of this material and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 0.65 mmol) in dry DMF (1.3 ml) were heated in a microwave reactor to 150° C. for 15 min. The mixture was concentrated in vacuo, and the residue submitted to column chromatography, followed by conversion to the hydrochloride salt (G1) to give the title compound as a faint yellow solid (0.11 g): NMR (1H, d4-MeOH): δ 7.76 (d, 1H), 7.62 (s, 1H), 7.57 (s, 1H), 7.24 (dd, 1H), 7.11 (d, 1H), 4.91 (s, 2H), 4.40 (t, 2H), 3.90 (s, 3H), 3.72-3.79 (m, 2H), 3.57 (t, 2H), 3.11 (t, 2H), 1.90-2.04 (m, 2H), 1.65-1.82 (m, 1H), 1.44-1.57 (m, 2H), 1.04 (d, 3H) (only data for major protonation isomer given). MS (m/z): 449 [MH]+ (2Cl).
2-Chloro-6-methylbenzoic acid (887 mg) was treated as described in procedure G3 to give the corresponding ester (929 mg).
Methyl 2-chloro-6-methyl benzoate (185 mg) was treated as described in procedure G2 to give the respective benzyl bromide as a colourless oil (213 mg) after flash chromatography (cyclohexane/ethyl acetate 95/5).
After heating to 60° C. for 2 h the bromide, 4-methoxy-3-(2-piperidin-1-ylethoxy)-phenylamine (P2, 0.1 mmol) and K2CO3 (0.1 mmol) in dry DMF (5 ml) and 1,4-dioxane (3 ml), 2,6-lutidine (0.76 mmol) was added. The mixture was heated to 95° C. for 4 h, then a saturated solution of NH4Cl was added, the mixture was extracted with dichloromethane, washed with brine and concentrated in vacuo. The residue was submitted to column chromatography (eluent: CH2Cl2/MeOH=95/5), the free base was then converted to the hydrochloride salt (G1), to give a light brown powder (70 mg). NMR (1H, CDCl3): δ 9.43 (bs, 1H), 7.75 (d, 1H), 7.65 (m, 2H), 7.53 (d, 1H), 7.32 (dd, 1H), 7.10 (d, 1H), 4.97 (s, 2H), 4.38 (t, 2H), 3.81 (s, 3H), 3.62 (d, 2H), 3.49 (t, 2H), 3.05 (t, 2H), 1.81 (m, 4H), 1.6 (m, 2H). MS (m/z): 401 [MH]+ (1Cl).
The title compound was prepared in an analogous manner to the compound of Example 7. NMR (1H, CDCl3): δ 10.15 (bs, 1H), 7.72 (m, 4H), 7.36 (dd, 1H), 7.12 (d, 1H), 5.0 (s, 2H), 4.41 (t, 2H), 3.81 (s, 3H), 3.55 (m, 2H), 3.49 (t, 2H), 3.05 (m, 2H), 1.81 (m, 4H), 1.4 (m, 2H). MS (m/z): 401 [MH]+ (1Cl).
The title compound was prepared in an analogous manner to the compound of Example 7. NMR (1H, CDCl3): δ 9.84 (bs, 1H), 7.75 (d, 1H), 7.70 (d, 1H), 7.67 (d, 1H), 7.55 (dd, 1H), 7.31 (dd, 1H), 7.06 (d, 1H), 4.95 (s, 2H), 4.33 (t, 2H), 3.76 (s, 3H), 3.52 (m, 2H), 3.46 (m, 2H), 3.0 (m, 2H), 1.72 (m, 4H), 1.35 (m, 2H). MS (m/z): 401 [MH]+ (1Cl).
To a solution of cis-2,6-dimethylpiperidine (1.48 mmol) and triethylamine (1.78 mmol) in dichloromethane (15 ml) was added chloroacetylchloride (1.78 mmol) at 0° C. After 5 h at 25° C. the mixture was washed with 5% NaHCO3, brine and evaporated in vacuo to give a yellow solid (433 mg). The α-cloroamide (200 mg) was reduced as described in procedure P4 to give 1-(2-chloroethyl)-2,6-dimethy-piperidine (139 mg).
To a solution of 5,7-dichloro-2-(3-hydroxy-4-methoxy-phenyl)-2,3-dihydroisoindol-1-one (P14, 78 mg, 0.24 mmol) in DMF (1.5 ml) at 25° C. were added K2CO3 (40 mg, 0.29 mmol) and 1-(2-chloroethyl)-2,6-dimethy-piperidine (51 mg, 0.29 mmol). The suspension was stirred at 40° C. for 6 h, then at room temperature for 72 h. The suspension was diluted with a saturated solution of NH4Cl and extracted with dichloromethane. The combined organic extracts were washed with brine and concentrated in vacuo. The residue was submitted to column chromatography (eluent: CH2Cl2/MeOH=9/1), the free base was then converted to the hydrochloride salt (G1), to give a light yellow powder (34 mg). NMR (1H, CDCl3): δ 9.66 (bs, 1H), 7.74 (d, 1H), 7.11 (d, 1H), 7.68 (d, 1H) 7.23 (dd, 1H), 7.06 (d, 1H), 4.94 (s, 2H), 4.32 (t, 2H), 3.77 (s, 3H), 3.66 (m, 2H), 3.44 (m, 2H), 1.7-1.45 (m, 4H), 1.85 (d, 2H), 1.38 (d, 6H). MS (m/z): 463 [MH]+ (2Cl).
2-Chloro-methylbenzoic acid (200 mg) was treated as described in procedure G3 to give the corresponding ester (204 mg).
Methyl 2-chloro-6-methyl benzoate (204 mg) was treated as described in procedure G4 to give a colourless oil (180 mg) which consisted of a mixture of starting material and chloro derivative as judged by NMR. After heating this mixture and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 0.44 mmol) in dry DMF (1.5 ml) and dry 1,4-dioxane (0.7 ml) to 95° C. for 1 h, 2,6-lutidine (0.37 mmol) was added. The mixture was heated to 95° C. for 2 h, concentrated in vacuo and the residue submitted to column chromatography (eluent: CH2Cl2/MeOH=95/5), Half of the free base was converted to the hydrochloride salt (G1), obtaining a faint yellow powder (27 mg): NMR (1H, d6-DMSO): δ 9.9/9.7 (b, 1H), 7.75 (d, 1H), 7.63 (m, 2H), 7.53 (d, 1H), 7.30 (dd, 1H), 7.09 (d, 1H), 4.86 (s, 2H), 4.37 (t, 2H), 3.79 (s, 3H), 3.61 (m, 2H), 3.48 (m, 2H), 3.05 (m, 2H), 1.80 (m, 2H), 1.60 (m, 1H), 1.45 (m, 2H), 0.92 (d, 3H) (only data for major protonation isomer given). MS (m/z): 415 [MH]+ (1Cl).
Examples 12 and 13 were prepared in analogy to Example 11:
NOTE: Compound (E12) was obtained according to Example 11 using procedure G2 instead of G4
To a solution of 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine (P5, 0.74 mmol) in dry toluene (2 ml) at 0° C. was added AlMe3 (2M in hexane, 2.0 mmol). After stirring the mixture at r.t. for 30 minutes, a solution of 7-methylphthalide (100 mg, 0.68 mmol) in CH2Cl2 (0.5 ml) was added at 0° C. The solution was stirring at r.t. for 12 h. After removing the solvent under reduced pressure, the residue was diluted with CH2Cl2 and washed with NaOH (1 M) and brine. The organic phase was dried over Na2SO4, filtered and concentrated to dryness in vacuo. The crude was submitted to column chromatography to give a yellow oil (225 mg). To a solution of this material (168 mg, 0.4 mmol) in THF (1.5 ml) at 0° C. were added PPh3 (0.8 mmol) and diisopropyl azodicarboxylate (0.8 mmol). The solution was stirred at 25° C. for 8 h, the solvent removed under reduced pressure and the residue purified by flash chromatography (CH2Cl2/MeOH=9/1) followed by conversion to hydrochloride salt (G1) to give the title compound (89 mg): NMR (1H, (CD3)2CO): δ 7.87 (d, 1H), 7.48 (m, 2H), 7.42 (dd, 1H), 7.26 (dd, 1H), 7.05 (d, 1H), 4.92 (s, 2H), 4.69 (t, 2H), 3.86 (s, 3H), 3.65/3.1 (m/m, 2/2H), 3.47 (t, 2H), 2.70 (s, 3H), 1.9/1.8 (m/m, 2/2H), 1.70 (m, 1H), 0.99 (d, 3H) (only data for major protonation isomer given, hydrochloride proton not observed). MS (m/z): 395 [MH]+.
The title compound (36 mg) was obtained from 6,7-difluorophthalide (P11) (100 mg, 0.59 mmol) in analogy to Example 14: NMR (1H, d6-DMSO): δ 9.35 (bs, 1H), 7.79 (m, 2H), 7.66 (d, 1H), 7.31 (dd, 1H), 7.08 (d, 1H), 4.94 (s, 2H), 4.31 (t, 2H), 3.76 (s, 3H), 3.58/3.27 (m/m, 2/2H), 3.47 (m, 2H), 1.80/1.37 (m/m, 2/2H), 1.57 (m, 1H), 0.89 (d, 3H) (only data for major protonation isomer given). MS (m/z): 417 [MH]+.
To a solution of 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine (P5, 4.2 mmol) in dry CH2Cl2 (20 ml) at 0° C. was added AlMe3 (2M in hexane, 6.2 mmol). After 15 minutes 5,6-dichlorophthalide (P13, 0.83 g) was added and the solution allowed to warm to 25° C. After 1.5 h additional 5,6-dichlorophthalide (P13, 0.07 g) was added. After additional 30 min NaOH (1 M aqueous) and CH2Cl2 were added. The resulting slurry was filtered through Celite, washing with some CH2Cl2. The CH2CO2 layer (ca. 100 ml) was found to contain only a small fraction of the amide intermediate. The filtration residues and the aqueous phase were extracted using 1:4 isopropanol:EtOAc, filtered, the organic layer washed (brine), dried (Na2SO4), filtered and concentrated to dryness in vacuo to provide the amide intermediate (1.6 g) as an off-white solid. To this material was added PPh3 (1.3 g) and THF (dry, 13 ml) followed by slow addition over 10 min at 50° C. of diisopropyl azodicarboxylate (1.0 ml). After 20 min water (3 drops) was added, after additional 5 min volatiles evaporated and the residue submitted to column chromatography followed by salt formation (G1) to give the title compound as a colourless film (0.37 g material of high purity besides 0.93 g material of lesser purity): NMR (1H, d6-DMSO): δ 9.5 (bs, 1H), 8.00 (s, 1H), 7.95 (s, 1H), 7.70 (d, 1H), 7.33 (dd, 1H), 7.10 (d, 1H), 4.90 (s, 2H), 4.35 (t, 2H), 3.79 (s, 3H), 3.61 (m, 2H), 3.50 (m, 2H), 3.04 (m, 2H), 1.80 (m, 2H), 1.60 (m, 1H), 1.40 (m, 2H), 0.92 (d, 3H) (only data for major protonation isomer given). MS (m/z): 449 [MH]+ (2Cl).
A solution of 3-fluorophthalic anhydride (166 mg, 1 mmol) and 4-methoxy-3-[2-(piperidin-1-yl)-ethoxy]-phenylamine (P2, 1 mmol) in dry DMF (1 ml) were heated in a microwave reactor to 200 W, 150 PSI for 10 min. After removing the solvent under reduced pressure, the residue was diluted with Et2O/CH2Cl2 (4/1) and washed with water. The organic phase was dried over Na2SO4, filtered and concentrated to dryness in vacuo. The crude was submitted to column chromatography (CH2Cl2:MeOH=9:1) to give the corresponding phthalimide derivative (300 mg); MS (m/z): 399 [MH]+.
To a solution of this material (185 mg, 0.46 mmol) in dry THF (4 ml) at 0° C. was added NaBH4 (1.2 mmol). The solution was cooled at room temperature and stirred for 8 h. After removing the solvent under reduced pressure, the residue was diluted with CH2Cl2 and washed with sat. aqueous NaHCO3 and brine. The organic phase was dried over Na2SO4, filtered and concentrated to dryness in vacuo. The crude was submitted to column chromatography (CH2Cl2:MeOH=9:1) to give a yellow oil (40 mg, 0.1 mmol)); MS (m/z): 401 [MH]+. To this material was added CH2Cl2 (2 ml), Et3SiH (0.8 mmol) and TFA (4 mmol). After 12 hours at room temperature volatiles were evaporated and the residue diluted with CH2Cl2 and washed with sat. aqueous NaHCO3 and brine. The organic phase was dried over Na2SO4, filtered and concentrated to dryness in vacuo. The crude was submitted to column chromatography (CH2Cl2:MeOH=9:1) to give a mixture of compounds (22 mg) as judged by NMR.
The title compound (1.5 mg) was obtained from this mixture of isomers by HPLC purification: NMR (1H, (CD3)2CO): δ 13.1 (bs, 1H), 7.80 (d, 1H), 7.67 (m, 1H), 7.50 (m, 2H), 7.23 (t, 1H), 7.07 (d, 1H), 5.05 (s, 2H), 4.69 (bs, 2H), 3.87 (s, 3H), 3.64 (bs, 2H), 3.47 (bs, 2H), 3.09 (bs, 2H), 2.18 (m, 2H), 1.85 (m, 2H), 1.50 (m, 2H).
The title compound (4 mg) was obtained by HPLC purification from the mixture of compounds described in the preparation of Example 17: NMR (1H, (CD3)2CO): δ 7.86 (d, 1H), 7.7/7.6 (m, 2H), 7.55 (m, 2H), 7.43 (t, 1H), 7.07 (d, 1H), 5.10 (s, 2H), 4.70 (t, 2H), 3.88 (s, 3H), 3.66 (d, 2H), 3.49 (t, 2H), 3.12 (t, 2H), 2.20 (m, 2H), 1.85 (m, 2H), 1.35 (m, 2H), (hydrochloride proton not observed).
Methyl 2,4,6-trimethyl benzoate (12 mmol) was treated as described in procedure G2 to give a colourless oil (2.6 g) which consisted of a mixture of compounds as judged by NMR. A portion of this material (0.22 g) and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 1.3 mmol) in dry DMF (2.5 ml) were heated in a microwave reactor to 150° C. for 15 min. The mixture was concentrated in vacuo, and the residue submitted to column chromatography, followed by conversion to the hydrochloride salt (G1). This was extracted with hot EtOAc. On standing at 25° C. the title compound crystallised from this solution as a faint yellow powder (15 mg): NMR (1H, d4-MeOH): δ 7.79 (d, 1H), 7.22-7.26 (m, 2H), 7.10-7.15 (m, 2H), ca. 4.8 (under H2O resonance, 2H), 4.41 (t, 2H), 3.91 (s, 3H), 3.76 (bd, 2H), 3.58 (t, 2H), 3.13 (bt, 2H), 2.66 (s, 3H), 2.43 (s, 3H), 1.90-2.04 (m, 2H), 1.70-1.82 (m, 1H), 1.45-1.57 (m, 2H), 1.05 (d, 5H)(only data for major protonation isomer given). MS (m/z): 409 [MH]+.
Examples 20-23 were prepared in analogy to Example 11 but using procedure G2 instead of G4:
7-Bromo-2-{4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]phenyl}-2,3-dihydroisoindol-1-one (free base, 0.15 g, prepared as described for Example 3) was heated at reflux in the presence of CuCN (61 mg) in dry DMF (7 ml) for 2 h. The mixture was allowed to cool, diluted with half conc. aqueous Na2CO3 and extracted with DCM (2 portions), the aqueous layer further diluted with brine and extracted again (EtOAc). The combined organic extracts were concentrated in vacuo, the residue purified by column chromatography followed by preparative HPLC, followed by conversion to the hydrochloride salt (G1) to give the title compound (14 mg) as a yellow solid: NMR (1H, d4-MeOH): δ 7.94 (2d, 2H), 7.90 (t, 1H), 7.83 (t, 1H), 7.28 (dd, 1H), 7.14 (d, 1H), 5.02 (s, 2H), 4.43 (t, 2H), 3.92 (s, 3H), 3.77 (d, 2H), 3.60 (t, 2H), 3.12 (bt, 2H), 1.93-2.02 (m, 2H), 1.66-1.82 (m, 1H), 1.43-1.58 (m, 2H), 1.04 (d, 3H)(only data for major protonation isomer given). MS (m/z): 406 [MH]+.
To a solution of 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine (P5, 0.18 g) in dry CH2Cl2 (2 ml) at 0° C. was added AlMe3 (2M in hexane, 0.54 ml). After 15 minutes 5,7-dichloro-6-methyl-phthalide (P17, 0.18 g) was added and the solution allowed to warm to 25° C. After 5 h silica gel, water (few drops), 10% conc. aqueous NH3/MeOH:CH2Cl2 1:10 were added with vigorous stirring, the resulting slurry removed by filtration and washed well with 10% conc. aqueous NH3/MeOH:CH2Cl2 1:10. The solution thus obtained was concentrated in vacuo and the residue submitted to column chromatography to provide the amide intermediate (0.26 g) as a red gum. To this material was added PPh3 (0.19 g) and THF (dry, 2 ml) followed by slow addition over 3 min at 50° C. of diisopropyl azodicarboxylate (0.14 ml). After 10 min the mixture was allowed to cool to 25° C., volatiles evaporated and the residue submitted to repeated column chromatography followed by salt formation (G1) to give the title compound as a colourless solid (0.15 g): NMR (1H, d4-MeOH): δ 7.76 (d, 1H), 7.65 (s, 1H), 7.23 (dd, 1H), 7.10 (d, 1H), 4.86 (s, 2H), 4.40 (t, 2H), 3.90 (s, 3H), 3.75 (d, 2H), 3.57 (t, 2H), 3.10 (t, 2H), 2.56 (s, 3H), 1.97 (d, 2H), 1.60-1.80 (m, 1H), 1.42-1.56 (m, 2H), 1.03 (d, 3H) (only data for major protonation isomer given). MS (m/z): 463 [MH]+ (2Cl).
The title compound (yellow solid, 0.14 g) was prepared in an analogous manner to the compound of Example 25, starting from 5,6-dichloro-7-methyl-phthalide (P19, 0.13 g): NMR (1H, d4-MeOH): δ 7.8 (d, 1H), 7.7 (s, 1H), 7.3 (dd, 1H), 7.17 (d, 1H), 4.9 (s, 2H), 4.45 (t, 2H), 3.94 (s, 3H), 3.8 (d, 2H), 3.6 (t, 2H), 3.15 (t, 2H), 2.6 (s, 3H), 2.0 (d, 2H), 1.80 (m, 1H), 1.45-1.62 (m, 2H), 1.09 (d, 3H) (only data for major protonation isomer given). MS (m/z): 463 [MH]+ (2Cl).
2-(Bromomethyl)-4,6-dichlorobenzoic acid methyl ester (200 mg, 50% purity, 0.33 mmol, cf. intermediate in Preparation 14), and 3-[2-(4,4-dimethylpiperidin-1-yl)ethoxy]-4-methoxyphenylamine hydrochloride (124 mg, 0.39 mmol) were dissolved in dry DMF (4 ml) and allowed to react in a microwave oven at 95° C. for 20 min. A saturated solution of NaHCO3 was added and the mixture was extracted with dichloromethane. The organic phase was washed with brine, dried over Na2SO4 and concentrated in vacuo. The residue was purified by flash chromatography (CH2Cl2/MeOH 9/1), to give 34 mg of the title compound, which was transformed into the hydrochloride salt (40 mg, white solid) by addition of HCl in Et2O. NMR (1H, DMSO-d6): δ 9.68 (bs, 1H), 7.74 (s, 1H), 7.71 (s, 2H), 7.27 (dd, 1H), 7.09 (d, 1H), 4.94 (s, 2H), 4.36 (t, 2H), 3.78 (s, 3H), 3.53 (t, 2H), 3.46 (d, 2H), 3.18 (q, 2H), 1.69 (t, 2H), 1.53 (d, 2H), 1.08 (s, 3H), 0.95 (s, 3H). MS (m/z): 463 [MH]+ (2Cl).
2-(Bromomethyl)-4,6-dichlorobenzoic acid methyl ester (232 mg, 60% purity, 0.46 mmol, cf. intermediate in Preparation 14) and 3-(2-azepan-1-ylethoxy)-4-methoxyphenylamine hydrochloride (140 mg, 0.46 mmol) were dissolved in dry DMF (4 ml) and reacted in a microwave oven at 130° C. for 20 min. A saturated solution of NaHCO3 was added and the mixture was extracted with dichloromethane. The organic phase was washed with brine, dried over Na2SO4 and concentrated in vacuo. The residue was purified by flash chromatography (CH2Cl2/MeOH 9/1), to give 77 mg of the title compound, which was converted into the hydrochloride salt (83 mg, white solid) by addition of HCl in Et2O. NMR (1H, CDCl3): δ 9.70 (bs, 1H), 7.72 (d, 1H), 7.70 (d, 1H), 7.68 (d, 1H), 7.23 (dd, 1H), 7.06 (d, 1H), 4.92 (s, 2H), 4.32 (t, 2H), 3.77 (s, 3H), 3.53 (q, 2H), 3.45 (m, 2H), 3.26 (m, 2H), 1.80 (m, 4H), 1.60 (m, 4H). MS (m/z): 449 [MH]+ (2Cl).
The title compound was prepared from 2-methyl-piperidine following the procedure used for Example 10. NMR (1H, DMSO-d6): δ 9.8 (bs, 1H), 7.78 (m, 3H), 7.28 (dd, 1H), 7.13 (d, 1H), 4.99 (s, 2H), 4.39 (m, 2H), 3.82 (s, 3H), 3.7-3.2 (m, 5H), 2.0-1.40 (m, 6H), 1.40 (d, 3H). MS (m/z): 449 [MH]+ (2Cl).
Ethyl 2,6-dimethyl-4-trifluoromethylpyridine-3-carboxylate (Katsumaya, I. et al., Synthesis 1997, 11, 1321-1324, 1.0 g) was treated as described in Procedure G2 to provide a colourless oil (0.55 g) containing ca. 50 mol % ethyl 2-bromomethyl-6-methyl-4-trifluoromethylpyridine-3-carboxylate. This material (0.33 g) and 4-methoxy-3-[2-(4-methyl-piperidin-1-yl)-ethoxy]-phenylamine hydrochloride (P5, 1.25 mmol) in dry DMF (2.5 ml) were heated in a microwave reactor to 150° C. for 15 min. The mixture was concentrated in vacuo, and the residue submitted to column chromatography, followed by trituration with MeOH:H2O 5:1 and subsequently a small volume of MeOH (ca. 3 ml), followed by conversion to the hydrochloride salt (G1) to give the title compound as an orange solid (0.15 g): NMR (1H, d4-MeOH): δ 7.84 (d, 1H), 7.77 (s, 1H), 7.32 (dd, 1H), 7.18 (d, 1H), 5.04 (s, 2H), 4.47 (t, 2H), 3.95 (s, 3H), 3.81 (d, 2H), 3.63 (t, 2H), 3.14 (t, 2H), 2.80 (s, 3H), 1.99 (d, 2H), 1.70-1.90 (m, 1H), 1.45-1.60 (m, 2H), 1.09 (d, 3H) (only data for major protonation isomer given). MS (m/z): 464 [MH]+.
The title compound was prepared in analogy to Example 11 but using procedure G2 instead of G4: NMR (1H, d6-DMSO): δ 7.77 (m, 2H), 7.32 (dd, 1H), 7.18 (d, 1H), 5.05 (s, 2H), 4.45 (t, 2H), 3.94 (s, 3H), 3.81/3.18 (d/t, 4H), 3.60 (t, 2H), 2.05/1.59 (d/t, 4H), 1.78 (m, 1H), 1.09 (d, 3H) (only data for major protonation isomer given). MS (m/z): 467 [MH]+ (2Cl).
| Number | Date | Country | Kind |
|---|---|---|---|
| 0308025.6 | Apr 2003 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP04/03678 | 4/5/2004 | WO | 9/26/2006 |
