Claims
- 1. A method for the detection of a pathogen in a sample which comprises the steps of:
- (a) providing a reagent complex comprising a solid support labeled with a fluorescent moiety and having attached thereto a single-stranded DNA probe to which is annealed a complementary DNA labeled with a fluorescent quencher;
- (b) contacting the reagent complex with a sample under conditions in which a nucleotide sequence of the pathogen, if present in the sample, hybridizes to the single-stranded DNA probe and displaces the DNA labeled with the fluorescent quencher; and
- (c) detecting an increase in fluorescent signal from the solid support as indicative of the presence of the pathogen in the sample.
- 2. The method of claim 1, wherein the solid support is a microsphere.
Parent Case Info
This application is a substitute of application Ser. No. 07/773,371, filed Oct. 8, 1991.
GOVERNMENT INTEREST
The invention described herein may be manufactured, used, and licensed by or for the United States Government.
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