Claims
- 1. A computer-readable product embodying a program for execution by a computer to identify a plurality of molecular tags by determining locations of peaks in electropherogram data correlated with such tags, the program comprising instructions for:
(a) reading electropherogram data from a storage medium, the electropherogram data obtained by electrophoretic separation of the plurality of molecular tags and one or more electrophoretic standards, each electrophoretic standard and molecular tag having a different electrophoretic mobility such that upon electrophoretic separation each electrophoretic standard and molecular tag forms a distinct peak in the electropherogram data, and each peak of such one or more electrophoretic standards and molecular tags having a migration interval and each migration interval having a mean; (b) determining a peak location of at least one electrophorctic standard within a migration interval in the electropherogram data; (c) determining peak locations of peaks within a migration interval in the electropherogram data closest to an electrophoretic standard or a qualified peak in a qualified peak set, the peak locations being relative to the location of the closest electrophoretic standard or qualified peak, and correlating with a molecular tag or an electrophoretic standard a peak within the migration interval whose location is closest to the mean of the migration interval; (d) determining a peak signal-to-noise ratio of the peak and adding the peak to the qualified peak set if the peak signal-to-noise ratio is greater than or equal to 1.5; and (e) repeating steps (c) and (d) until a peak location is correlated to every molecular tag having a peak in the electropherogram data.
- 2. The computer-readable product of claim I wherein said step of reading includes prvding at least two said electrophoretic standards, a first electrophoretic standard having an electrophoretic mobility higher than any of said electrophoretic mobilities of said molecular tags and a second electrophoretic standard having an electrophoretic mobility lower than any of said electrophoretic mobilities of said molecular tags.
- 3. The computer-readable product of claim 2 wherein each of said locations of said peaks correlated with said molecular tags is determined as a ratio of the difference between a migration time of such peak and a migration time of said closest electrophoretic standard or qualified peak and the difference between a migration time of said second electrophoretic standard and the migration time of said closest electrophoretic standard or qualified peak.
- 4. The computer-readable product of claim 1 further including a step of determining for each said molecular tag a peak size and correlating the peak size to an amount of said molecular tag.
- 5. A computer system for identifying a plurality of molecular tags by determining locations of peaks in electropherogram data correlated with such molecular tags, the computer system comprising:
an input device for inputting electropherogram data from a storage medium, the electropherogram data obtained by electrophoretic separation of the plurality of molecular tags and one or more electrophoretic standards, each electrophoretic standard and molecular tag having a different electrophoretic mobility such that upon electrophoretic separation each electrophoretic standard and molecular tag forming a distinct peak in the electropherogram data, and each peak of such one or more electrophoretic standards and molecular tags having a migration interval and each migration interval having a mean; a memory for storing the electropherogram data; and a processing unit programmed for: (a) determining a peak location of at least one electrophoretic standard within a migration interval in the electropherogram data; (b) determining peak locations of peaks within a migration interval in the electropherogram data closest to an electrophoretic standard or a qualified peak in a qualified peak set, the peak locations being relative to the location of the closest electrophoretic standard or qualified peak, and correlating with a molecular tag or an electrophoretic standard a peak within the migration interval whose location is closest to the mean of the migration interval; (c) determining a peak signal-to-noise ratio of the peak and adding the peak to the qualified peak set if the peak signal-to-noise ratio is greater than or equal to 1.5; (d) repeating steps (b) and (c) until a peak location is correlated to every molecular tag having a peak in the electropherogram data.
CROSS-REFERENCE TO RELATED APPLICATIONS AND PATENTS
[0001] This application claims priority from U.S. provisional application Ser No. 60/369,652 filed 2 Apr. 2002, which is incorporated herein by reference in its entirety.
[0002] This invention relates to method for detecting and/or measuring one or more analytes in an assay by the generation and electrophorectic separation of molecular tags.
Provisional Applications (1)
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Number |
Date |
Country |
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60369652 |
Apr 2002 |
US |