Described below are a confocal spectrometer and a method for imaging in a confocal spectrometer.
Confocal spectrometers operate on the basis of optical systems which have a common focus. In this way, a spatially pointwise measurement of scattered light can be carried out on an object to be imaged. Single-channel spectrometers to date generally use a linear camera in order to acquire the spectrum for one channel. It is therefore possible to acquire a spatially resolved image of the object only by scanning the object surface, that is to say by a time-based scan.
Multichannel spectrometers use a camera chip for linear sampling of a surface, spectral resolution taking place on the camera chip in a direction perpendicular to the spatial resolution. Such systems are also known as so-called hyperspectral imaging systems. In these systems as well, scanning of the object surface is necessary for imaging acquisition of the object.
Document EP 1 984 770 B1 discloses a confocal spectrometer system, encoding of a profile of an object being carried out by the spectral variation of a polychromatic light source. To this end, imaging optics with chromatic aberration are used in order to generate a wavelength-dependent position of the imaging focus along the optical axis.
Document DE 697 300 30 T2 discloses a confocal spectroscopic imaging system in which a modulator for imaging an illumination pattern onto an object to be imaged is used, so that spatial resolution of the object is possible by the illumination pattern sequence.
There is a need for an imaging spectrometer which, for a stationary object, delivers a spectrum of the reflected or scattered light for each image point in order to generate an image contrast.
One aspect is a confocal spectrometer having a broadband light source, a first aperture device arranged in front of the light source and having a first slit grid of a main slit direction, which is configured in order to generate a slit-shaped pattern of the light source, first imaging optics, which are configured in order to focus the slit-shaped pattern of the light source onto an object to be imaged, and a detector system, which has a detector apparatus, which is configured in order to acquire the light reflected by the object in order to generate a spectrally resolved image of the object, second imaging optics, which are configured in order to focus the reflected light onto the detector apparatus, and a dispersion element, which is arranged in front of the second imaging optics and is configured in order to spectrally disperse the light reflected by the object along a dispersion axis perpendicular to the optical axis of the second imaging optics.
One essential idea of the method is to permit full spatial resolution at the same time as full spectral resolution of the image of an object in a spectrometer. To this end, the confocal technique is used with an imaging aperture device, the aperture device having a slit pattern which projects a slit grid onto the entire object. When the projected slit grid reflected by the object is imaged confocally onto a detector apparatus, spectral resolution can be carried out in the intermediate spaces of the slit grid. This makes possible a spectrally dispersive element, which can image the reflected light with spectral resolution into the respective slit intermediate spaces.
According to one embodiment, the detector system may furthermore include a second aperture device having a second slit grid of the main slit direction of the first slit grid, which is arranged between the dispersion element and the detector apparatus and is configured in order to make a spectral selection of the reflected light striking the detector apparatus.
According to an embodiment, the second aperture device may be displaceable along the dispersion axis direction. This advantageously permits mechanical selection of a wavelength, to be imaged, of the reflected light.
According to another embodiment, the second slit grid may have a multiplicity of first slits, which are offset in relation to the slits of the first slit grid by a first predetermined distance perpendicularly to the main slit direction, and a multiplicity of second slits, which are offset in relation to the slits of the first slit grid by a second predetermined distance, different to the first distance, perpendicularly to the main slit direction. This offers the advantage that, for certain applications in which particular wavelengths of the reflected light are of interest, for example medical imaging methods in surgery or tissue diagnosis, a predefined selection of a number of wavelengths can be carried out without the second aperture device having to be mechanically displaced along the dispersion axis. In this way, complete spatially and spectrally resolved images of an object can be acquired confocally in a very short time.
According to another embodiment, the first aperture device may include a multiplicity of cylindrical lenses, which are configured in order to image light of the light source onto the slits of the first slit grid. This offers the advantage that the light intensity of the light source can be used maximally, since almost all of the light of the light source can be collimated onto the slit grid.
According to another embodiment, the spectrometer may furthermore include a beam splitter element, which is arranged in the beam path of the first imaging optics and is configured in order to deviate the reflected light of the object out of the beam path of the first imaging optics into the detector system. In this way, physical decoupling of the detector system from the imaging system is advantageously possible.
According to another embodiment, the dispersion element may include a prism, a diffraction grating, an interference filter or an acousto-optical modulator.
According to another embodiment, the detector apparatus may include a CCD sensor array, a CMOS sensor array or an avalanche photodiode array. In this case, the detector apparatus may be configured in order to spectrally resolve reflected image points of the object along an array axis. This is particularly advantageous, since individual image pixels of the object can respectively be imaged onto a subarray of pixels of the array of the detector apparatus. With the aid of this subarray of pixels, both spatially and spectrally resolved images of an object can be produced, which entails information enrichment in spatial representation of objects, particularly for medical imaging applications.
According to another embodiment, the light source may be a white light source. In this way, advantageously, at any time in the imaging each spectral component is equally available for acquisition in the reflected light spectrum. In particular, different wavelengths of the reflected light spectrum can thus be acquired simultaneously.
According to another aspect, described below is a method for imaging in a confocal spectrometer, by imaging a broadband light source onto a first aperture device having a first slit grid of a main slit direction for generating a slit pattern, focusing the slit pattern onto an object to be imaged, spectrally dispersing the light reflected by the object along a dispersion axis which is perpendicular to the main slit direction, focusing the spectrally dispersed reflected light onto a detector apparatus, and detecting the reflected light in the detector apparatus in order to generate a spectrally resolved image of the object.
According to one embodiment, the method may include focusing the spectrally dispersed reflected light onto a second aperture device having a second slit grid with the main slit direction of the first slit grid, which is arranged in front of the detector apparatus.
According to an embodiment, the method may include displacing the second aperture device along the dispersion axis direction in order to select the wavelength of the detected light. In this way, different wavelengths of the reflected light spectrum can be selected for acquisition in a controlled way during the spectroscopic acquisition.
These and other aspects and advantages will become more apparent and more readily appreciated from the following description of the exemplary embodiments and configurations with reference to the appended drawings, in which:
The described configurations and refinements may, where expedient, be combined with one another in any desired way. Further possible configurations, refinements and implementations also include not explicitly mentioned combinations of the features described above or below in relation to the exemplary embodiments.
The appended drawings are intended to impart further understanding of the embodiments. They illustrate embodiments and serve in connection with the description to explain principles and concepts. Other embodiments and many of the advantages mentioned are revealed with reference to the drawings. The elements of the drawings are not necessarily shown true to scale with respect to one another. References which are the same denote components which are the same or have a similar effect. The direction terminology used below with terms such as “up”, “down”, “right”, “left”, “front”, “rear”, etc. is used merely for easier understanding of the drawings, and represents no restriction of generality.
Reference will now be made in detail to the preferred embodiments, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
The imaging system 1 has a light source 11. The light source 11 may be a broadband or polychromatic light source 11, that is to say a light source 11 which emits light over a wide frequency or wavelength range. For example, the light source 11 may be a white light source, a Globar, a Nernst lamp, a nickel-chromium filament, a halogen gas discharge lamp, a xenon gas discharge lamp, a superluminescent diode, an LED or a similar polychromatic light source. Furthermore, the spectral wavelength range which the emission spectrum of the light source 11 covers may lie in the UV range, in the visible light range and/or in the infrared range.
The light emitted by the light source 11 may be collimated by a lens 12 to form a parallel ray bundle and directed onto a first aperture device 14. The first aperture device 14 may have a slit-shaped grid. An example of such a slit-shaped grid is represented schematically in
In the imaging system 1, provision may be made for the collimated light to be focused by cylindrical lenses 13a in a cylindrical lenses arrangement 13 onto the slits of the slit grid 14k of the first aperture device 14. In this case, one of the cylindrical lenses 13a may respectively be assigned to each slit 14k. The cylindrical lenses arrangement 13 may, for example, be connected integrally to the first aperture device 14. By use of the cylindrical lenses 13, a larger fraction of the light of the light source 11 can be used for projection of the slit grid 14k of the first aperture device 14 onto the object 16.
The light passing through the first aperture device 14 may be focused by first imaging optics 15 onto the object 16. In this case, the object 16 is illuminated on its surface at a focal point 16a by the light of the light source 11. The illumination is carried out in the pattern of the slit structure of the first aperture device 14. To this end, for example, tube optics 15a and an objective lens device 15b may be used.
The light scattered or reflected by the object 16 is guided back into the imaging optics 15 by the objective lens device 15b. A beam splitter element 15c, which may for example be a polarizing beam splitter, an interference filter or a similar optical element that splits an incident light beam, may be arranged in the imaging optics 15. The scattered or reflected light is deviated into the detector system 2 via a beam path having an optical axis.
The detector system 2 includes a spectrally dispersive element 21, which causes spectral splitting of the light, reflected in broadband fashion by the object, along a dispersion direction. The dispersion direction axis D may in this case be perpendicular to the optical axis A, so that the spectral information of the scattered or reflected light is resolved along the dispersion direction axis D. The dispersion element 21 may, for example, be a prism, a diffraction grating, a holographic grating, a blazed grating, an acousto-optical modulator, an interference filter or a similar element.
The spectrally dispersed light may be focused by a focusing lens 22 onto a second aperture device 23. The second aperture device 23 may, in particular, have a slit grid similar to the first aperture device 14. The spectrally dispersed light is imaged through the second aperture device 23 onto a detector apparatus 24.
It may in this case be possible to have a one-dimensional sensor array, for example a CCD sensor array, a CMOS sensor array, an avalanche photodiode array or a similar one-dimensional matrix of photosensitive sensor elements as the detector apparatus 24. The detector apparatus 24 may in this case be displaced together with the second aperture device 23 along the dispersion direction axis D, so that a fraction of the spectrally dispersed light of the dispersion element 21 can respectively be selected by the second aperture device 23 and imaged onto the detector apparatus 24.
As an alternative, it may also be possible not to use a second aperture device 23. In this case, a two-dimensional sensor array, for example a CCD sensor array, a CMOS sensor array, an avalanche photodiode array or a similar two-dimensional matrix of photosensitive sensor elements may be used as the detector apparatus 24. In this way, each wavelength fraction of the spectrally dispersed light can be acquired along the array axis which extends parallel to the dispersion direction axis D. To this end, the spectrally dispersed light may be focused directly by the focusing lens 22 onto the detector apparatus 24. An exemplary embodiment of such a detector apparatus 24 is schematically represented for illustration in
Two neighbor pixels 26k+1,n and 26k,n+1 of the subarray 26k,n are shown in a dashed contour. The neighbor pixel 26k+1,n in this case images an image point of the object 16 following on from the pixel 26k,n in the lateral spatial direction, while the neighbor pixel 26k,n+1 images an image point of the object 16 following on from the pixel 26k,n in the vertical spatial direction. Within each subarray, spectral resolution of the respective image point of the object 16 can take place along the array axis S, since the spectrally dispersive element 21 causes spectral splitting of the object image along the dispersion direction axis D, which may for example coincide with the array axis S. The selection of the spectral range, to be determined, of the reflected light may, for example, take place within the subarray 26k,n by the electronic drive of the spectrally assigned pixels respectively lying along the array axis S.
When a second aperture device 23 is used, only that spectral part of the spectrally dispersed light which corresponds to the lateral offset of the second aperture device 23 along the dispersion direction axis D in relation to the position of the first aperture device 13 is deviated onto the detector apparatus 24. In other words, a spectral selection of the reflected light can be made by a lateral offset of the slit grid of the second aperture device 23, so that only a part of a two-dimensional detector apparatus 24 is illuminated.
The spectral image acquisition may, for example, be carried out by a scanning lateral offset movement of the aperture device 23. As an alternative, it may be possible to make a spectral selection by an electronic drive of the pixels of the detector apparatus 24.
For certain applications, for example in the medical field, it may be expedient to make a preselection of spectral ranges to be resolved.
Next is focusing 202 of the slit pattern onto an object to be imaged is carried out. Then, spectral dispersion 203 of the light reflected by the object takes place along a dispersion axis, which is perpendicular to the main slit direction. The spectral dispersion may for example be carried out with the aid of a prism, a diffraction grating, an interference filter or an acousto-optical modulator.
Fourth is focusing 204 of the spectrally dispersed reflected light onto a detector apparatus may be carried out. In this case, it may be possible to focus the spectrally dispersed light onto a second aperture device having a second slit grid with the main slit direction of the first slit grid. It is in this case possible for a part of the light reflected by the object to be deviated with a beam splitter element out of the beam path of the imaging of the slit pattern.
Finally, detection 205 of the reflected light is carried out in order to generate a spectrally resolved image of the object. The detection of the reflected light may for example be carried out with a two-dimensional CCD sensor array, a CMOS sensor array or an avalanche photodiode array. In this case, the reflected image points of the object may be spectrally resolved along an array axis. When a second aperture device is used, in order to select the wavelength of the detected light it may be possible to displace the second aperture device along the dispersion axis direction in order to select the wavelength of the detected light. In this case, a one-dimensional sensor array may also be used as the detector apparatus, for example a sensitive one-dimensional avalanche photodiode array which can be displaced together with the second aperture device along the dispersion axis direction.
The imaging system 1 has a light source 11. The light source 11 may be a broadband or polychromatic light source 11, that is to say a light source 11 which emits light over a wide frequency or wavelength range. For example, the light source 11 may be a white light source, a Globar, a Nernst lamp, a nickel-chromium filament, a halogen gas discharge lamp, a xenon gas discharge lamp, a superluminescent diode, an LED or a similar polychromatic light source. Furthermore, the spectral wavelength range which the emission spectrum of the light source 11 covers may lie in the UV range, in the visible light range and/or in the infrared range.
The light emitted by the light source 11 may be collimated by a lens 12 to form a parallel ray bundle and directed onto a first aperture device 34. The first aperture device 34 may have a structured arrangement of a multiplicity of holes, so-called pinholes. One example of such a structured arrangement may be a Nipkow disk, as is represented by way of example in
The first aperture device 34 in
In the imaging system 1, provision may be made for the collimated light to be focused by lenses 33a in a lens arrangement 33 onto the holes of the first aperture device 34. In this case, one of the lenses 33a may respectively be assigned to each hole 34k. The lens arrangement 33 may, for example, be connected integrally to the first aperture device 34. By virtue of the lenses 33, a higher fraction of the light of the light source 11 can be used for projection of the structure of holes 34k of the first aperture device 34 onto the object 16.
The light passing through the first aperture device 34 may be focused by first imaging optics 15 onto the object 16. In this case, the object 16 is illuminated on its surface at a focal point 16a by the light of the light source 11. The illumination is carried out by rotation of the first aperture device 34 over the entire field of view of the object 16. To this end, for example, tube optics 15a and an objective lens device 15b may be used.
The light scattered or reflected by the object 16 is guided back into the imaging optics 15 by the objective lens device 15b. A beam splitter element 15c, which may for example be a polarizing beam splitter, an interference filter or a similar optical element that splits an incident light beam, may be arranged in the imaging optics 15. The scattered or reflected light is deviated into the detector system 2 via a beam path having an optical axis A.
The detector system 2 includes a spectrally dispersive element 41, which causes spectral splitting of the light, reflected in broadband fashion by the object, along a dispersion direction. The dispersion direction axis D may in this case be perpendicular to the optical axis A, so that the spectral information of the scattered or reflected light is resolved along the dispersion direction axis D. The dispersion element 41 may, for example, be a prism, a diffraction grating, a holographic grating, a blazed grating, an acousto-optical modulator, an interference filter or a similar element.
The spectrally dispersed light may be focused by a focusing lens 22 onto a second aperture device 43. The second aperture device 43 may, in particular, have a hole 35k pattern similar to the first aperture device 34. The spectrally dispersed light is imaged through the second aperture device 43 onto a detector apparatus 24. The detector apparatus 24 may for example include a two-dimensional CCD sensor array, a CMOS sensor array, an avalanche photodiode array or a similar matrix of photosensitive sensor elements.
The second aperture device 43 can in this case rotate about an axis B, so that the rotation of the holes coincides with that of the holes 35k of the first aperture device 34. In this way, light reflected or scattered by the object 16 can be imaged confocally with the first aperture device 43. This means that depth selection can be carried out, since only image points on the object 16 which lie within the focal depth of the focal point 16 can be imaged through the second aperture device 43.
By the spectral dispersion of the dispersion element 41 along the dispersion axis D, a lateral offset of the second aperture device 43 along this dispersion direction axis D can be carried out for spectral selection of the confocally acquired light of the object 16. In other words, at the same time as full lateral resolution of the object 16, spectral resolution of the object 16 is possible at the same time by adjusting a lateral offset between the first aperture device 34 and the second aperture device 43 with respect to the optical axis A.
As an alternative, it is also possible to achieve a displacement of the spectrum with respect to the optical axis by manipulation of the dispersion element 41. For example, a prism 41 may be rotated or an acousto-optical modulator 41 may be driven accordingly.
To this end, a polarizer 41, which linearly polarizes the light emerging from the light source 11, may be provided behind the lens 12. The incident light passes through the beam splitters 45a and 45b in a straight line when the latter are polarization-dependent beam splitters, for example s-polarizing beam splitters. Due to the p-polarizing beam splitters 45c and 45d and the mirror elements 45e and 45f, the incident light is guided along the beam path W to the object. With the aid of a lambda/4 plate 46, phase rotation of the polarization through 90° can be carried out.
The light reflected or scattered by the object is phase-shifted again through 90° by the lambda/4 plate 46, so that the reflected light can pass unimpeded in a straight line through the p-polarizing beam splitters 45d and 45c, and is deviated along the beam path X at the beam splitter 45b. The optical path lengths over the beam paths W and X may in this case be the same. In the beam path X, there is a spectrally dispersive element 43, for example a prism, which causes spectral splitting of the reflected or scattered light of the object. By rotation of the beam splitter 45a, it is possible to carry out spectral selection of the reflected or scattered light which is guided via the aperture device 34 onto a beam splitter 42 and deviated from there through a focusing lens 22 onto the detector apparatus 24. As an alternative, it may be possible to achieve wavelength selection for imaging onto the detector apparatus 24 by rotation of the spectrally dispersive element 41.
First, imaging 501 of a broadband light source takes place through a rotatable aperture device having a structured arrangement of a multiplicity of holes. The light source may in this case be a white light source or a polychromatic light source. The rotatable aperture device may, for example, include a Nipkow disk. Then, focusing 502 of the image of the structured arrangement of the multiplicity of holes onto an object to be imaged takes place. In this case, the imaging of the light source may be imaging of the light source on the structured arrangement of the multiplicity of holes with the aid of a multiplicity of lenses assigned to the holes.
Next, spectral dispersion 503 of the light reflected by the object is carried out with the aid of a dispersion element, for example a prism, a diffraction grating, an interference filter, or an acousto-optical modulator. Fourth, focusing 504 of the spectrally dispersed reflected light onto a rotatable aperture device having a structured arrangement of a multiplicity of holes is carried out. In this case, the rotatable aperture device may be displaced perpendicularly to the optical axis of the spectrometer for selection of the wavelength of the detected light. As an alternative, the dispersion element may be displaced perpendicularly to the optical axis of the spectrometer for selection of the wavelength of the detected light.
Finally, detection 505 of the reflected light passing through the rotatable aperture device is carried out in order to generate a spectrally resolved image of the object. The detection of the reflected light may be carried out with the aid of a CCD sensor array, a CMOS sensor array or an avalanche photodiode array, so that the reflected image points of the object can be spectrally resolved along an array axis.
Although principles, technical effects and features have only been presented and explained with reference to some of the figures, it is however readily possible to apply configuration variants and modifications of an embodiment explained in one of the figures to any other of the embodiments of the other figures.
Described above is a confocal spectrometer having a broadband light source, a first aperture device arranged in front of the light source and having a first slit grid of a main slit direction, which is configured in order to generate a slit-shaped pattern of the light source, first imaging optics, which are configured in order to focus the slit-shaped pattern of the light source onto an object to be imaged and a detector system, which includes a detector apparatus, which is configured in order to acquire the light reflected by the object in order to generate a spectrally resolved image of the object, second imaging optics, which are configured in order to focus the reflected light onto the detector apparatus, and a dispersion element, which is arranged in front of the second imaging optics and is configured in order to spectrally disperse the light reflected by the object along a dispersion axis perpendicular to the optical axis of the second imaging optics.
A description has been provided with particular reference to preferred embodiments thereof and examples, but it will be understood that variations and modifications can be effected within the spirit and scope of the claims which may include the phrase “at least one of A, B and C” as an alternative expression that means one or more of A, B and C may be used, contrary to the holding in Superguide v. DIRECTV, 358 F3d 870, 69 USPQ2d 1865 (Fed. Cir. 2004).
Number | Date | Country | Kind |
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102011083718.3 | Sep 2011 | DE | national |
This application is the U.S. national stage of International Application No. PCT/EP2012/067421, filed Sep. 6, 2012 and claims the benefit thereof. The International application claims the benefit of German Application No. 102011083718.3 filed on Sep. 29, 2011, both applications are incorporated by reference herein in their entirety.
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/EP2012/067421 | 9/6/2012 | WO | 00 | 3/28/2014 |