Claims
- 1. A method for constructing a rotatable culture vessel for containing a liquid culture medium, and defining an annular culture chamber, for use with conventional laboratory roller devices, the method comprising:
- subjecting a one piece, self-supporting and substantially rigid plastic molded annularly shaped housing having an inner cylindrically shaped wall and an outer cylindrically shaped wall forming therebetween an annular chamber disposed about a common longitudinal axis and an annular transverse end wall so as to have an open bore through said housing to bombardment means for producing microperforations through said inner and outer walls while rotating and moving the housing relative to the bombardment means to develop said microperforations along the length and about the periphery of said walls where such microperforations are sized for admitting oxygen in the atmosphere to the annular chamber while retaining the liquid culture media in the annular chamber.
- 2. The method as set forth in claim 1 wherein said bombardment means is a neutron generator.
- 3. The method as set forth in claim 1 wherein said bombardment means is a laser.
- 4. A method for constructing a rotatable culture vessel, defining an annular culture chamber for containing a liquid culture medium, for use with conventional laboratory roller devices comprising:
- selecting a porous material which is polymeric and hydrophobic as well as compatible to cell growth; and
- forming said culture vessel by sintering said material into a one piece plastic molded substantially rigid, self-supporting, annularly shaped housing with an inner cylindrically shaped wall and an outer cylindrically shaped wall forming a chamber of annular configuration therebetween disposed about a common longitudinal axis and an annular transverse end wall so as to have an open bore through said housing and;
- forming microperforations through said inner and outer walls along the length and about the periphery of said walls during the sintering of said material by sintering the material to fuse together particles of the plastic to obtain a desired porosity and pore diameter, where such microperforations are sized for admitting oxygen in the atmosphere through the outer wall to the annular chamber, and through the inner wall to the annular chamber, while retaining the liquid culture medium in the annular chamber.
- 5. The method of claim 1, wherein said step of subjecting said annularly shaped housing to said bombardment means comprises simultaneously subjecting both said inner and said outer cylindrically shaped walls to said bombardment means, for simultaneously forming said microperforations through both said inner and outer walls.
RELATED APPLICATIONS
This application is a division of U.S. patent application Ser. No. 07/996,263, filed Dec. 23, 1992, now U.S. Pat. No. 5,330,908.
ORIGIN OF THE INVENTION
The invention described herein was made by an employee of the United States Government and may be manufactured and used by or for the Government of the United States of America for governmental purposes without the payment of any royalties thereon or thereafter.
US Referenced Citations (9)
Foreign Referenced Citations (3)
Number |
Date |
Country |
63-23936 |
Jan 1988 |
JPX |
1089285 |
Nov 1967 |
GBX |
1375204 |
Nov 1974 |
GBX |
Divisions (1)
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Number |
Date |
Country |
Parent |
996263 |
Dec 1992 |
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