Claims
- 1. A method comprising continuous countercurrent chromatographic separation of analytes wherein the analytes are separated by size exclusion chromatography with no adsorption of the analytes by a sorbent, said separation being based upon calculating net flow ratios of a simulated moving bed method from parameters of checked, calculated effective porosities, residence times; and band broadening, thereby allowing stabilization of a raffinate front of the component retained the shortest in the region of Section IV.
- 2. A method according to claim 1, wherein the chromatography is by simulated moving bed chromatography.
- 3. A method according to claim 2, further comprising:calculating separation column residence times of separated substances and band broadening of substance peaks; obtaining data of batch chromatograms; calculating effective porosities of each separated substance and residence times from the data; estimating effective molecular diffusion coefficients from Stokes-Einstein relation and molecular weights of the separated substances; calculating band broadening from the effective molecular diffusion coefficients; checking the agreement of the calculated effective porosities, residence times, and band broadening with an observed elution profile, and optionally, improving matching of the calculated and experimental data by at least one iteration step; and calculating net flow rate ratios of the simulated moving bed method from parameters of the checked, calculated effective porosities, residence times, and band broadening.
- 4. A method according to claim 2, further comprising conducting the method with units wherein the units are connected by a two-way or multiport valve.
- 5. A method according to claim 2, wherein the separation is conducted under isocratic conditions.
- 6. A method according to claim 1, wherein the analytes are separated by diffusion with no sorbent adsorption.
- 7. A method according to claim 1, for purifying proteins from transgenic animal milk, proteins from blood plasma, plasmids, salts from feed mixes, loaded liposomes, peptides, or viruses.
- 8. A method according to claim 1, further comprising conducting the method with eight columns, wherein each column has a diameter of 26 mm and a length of 150 mm.
- 9. A method according to claim 1, wherein the size exclusion chromatography is gel permeation chromatography.
Priority Claims (1)
Number |
Date |
Country |
Kind |
198 58 892 |
Dec 1998 |
DE |
|
REFERENCE TO RELATED APPLICATIONS
This application is a 371 of PCT/EP99/09495 filed Dec. 4, 1999.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/EP99/09495 |
|
WO |
00 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO00/37156 |
6/29/2000 |
WO |
A |
US Referenced Citations (7)
Foreign Referenced Citations (2)
Number |
Date |
Country |
4316136 |
Nov 1994 |
DE |
9734918 |
Sep 1997 |
WO |
Non-Patent Literature Citations (1)
Entry |
Snyder, Introduction to modern liquid chromatography John Wiley, 1979, pages 484,485, and 663. |