Patent Application
20150316658
The present disclosure relates to a contrast agent for optical imaging, a use thereof and an apparatus using the same, and more particularly, to a contrast agent allowing for optical imaging even without having a fluorophore or a luminophore, a use thereof and an apparatus using the same.
With the recent rapid development, various molecular imaging techniques allow to observe many in vivo processes, which was impossible in the past. Especially, nuclear imaging using radioisotopes is widely used in basic biological studies using small animals, preclinical researches and clinical applications, based on the advantages of high sensitivity, good tissue penetration and excellent quantification. The radionuclides may be categorized into five groups depending on their decay modes. They are: α decay, β− decay, β decay, electron capture and isomeric transition. Among them, β+-decaying nuclides (18F, 11C, 13N, 15O, 64Cu, 124I and 68Ga), which emit positrons during radioactive decay, are used to construct positron emission tomography (PET) images. The nuclides that emit gamma rays with appropriate energy during isomeric transition may be used for a gamma camera or single photon emission computed tomography (SPECT). 99mTc is the representative example. In β− decay, an electron is emitted during the decay process. 3H, 14C, 35S, etc., which are widely utilized in radioisotope experiments in biological researches, belong to this category. Also, those widely used in radiation therapy using the emitted electrons, such as 32P, 131I, 89Sr, 90Y, 153Sm, 177Lu and 186Re, belong to this category. The nuclides that decay by electron capture include 111In, 123I, 125I, 201Tl and 67Ga. They may be used for gamma camera imaging since gamma ray is also emitted during the decay. As for the nuclides that undergo α decay, 211At finds its application in radiation therapy.
A variety of radiopharmaceuticals have already been developed for use in gamma cameras and SPECT, whereby images are acquired based on gamma radiation, PET, wherein positron-emitting nuclides are used, or the like. They are widely used for accurate diagnosis of various diseases including cancer. Besides, therapeutic radiopharmaceuticals using various β−- or α-emitting radionuclides are gradually extending their applications. However, unlike optical imaging, nuclear imaging is not easily applicable in basic pre-screening stages since it requires expensive instruments such as SPECT or PET and expert operators. Furthermore, nuclear imaging has lower temporal resolution and requires more time to detect high-energy gamma rays and collect data for constructing images, as compared to the optical imaging.
In contrast to the nuclear imaging, optical imaging such as fluorescence and bioluminescence imaging is easy to handle and the instrumentation is much less expensive than PET or SPECT. Therefore, many basic and biomedical studies employ optical imaging to visualize molecular processes in living organisms. The optical imaging provides high sensitivity and excellent temporal resolution. However, quantification based on optical signals is not accurate and has limited applications in clinics due to the absorption and scattering of light in the body and low tissue penetration resulting therefrom.
Accordingly, there have been various attempts to combine nuclear imaging and optical imaging having their strong and weak points, and hybrid type imaging probes have been developed using nanoparticles such as quantum dots and near-IR fluorophores. However, in order to obtain both nuclear and optical images using one contrast agent, a hybrid type probe prepared by conjugating a radionuclide for nuclear imaging with a fluorescent or bioluminescent probe for optical imaging is needed. Especially, whereas nuclear imaging requires only one radioisotope to be introduced, optical imaging requires that a fluorophore or a luminophore (e.g., luciferase) be attached to the radioisotope. However, since most fluorophores or luminophores are bulky metal complexes, aromatic organic compounds or nanoparticles such as quantum dots, they change the physicochemical properties of the target molecules in many cases and, thus, their use in living organisms is extremely restricted. Although Korean Patent Publication No. 2007-0029030 discloses nanoparticles coated with a silica shell to confer both optical and magnetic properties, introduction of an organic fluorophore is still needed to exhibit optical properties.
The present disclosure is directed to providing a contrast agent including a radionuclide, which allows optical imaging without a fluorophore or a luminophore.
The present disclosure is also directed to providing a contrast agent including a radionuclide and a fluorophore, which allows optical imaging without energy input from outside by absorbing the energy emitted from the radionuclide and emitting light through the fluorophore, leading to stronger emission.
The present disclosure is also directed to providing a method for acquiring optical images of a living organism in a noninvasive manner in real time, by injecting a contrast agent including a radionuclide into the living organism.
The present disclosure is also directed to providing a contrast agent for trimodality imaging that can be prepared easier without having to attach a fluorophore and provides excellent resolution with superior tissue penetration, as compared to the existing contrast agents for trimodality imaging.
The present disclosure is also directed to providing various apparatuses for acquiring optical images, capable of converting the energy of a charged particle emitted from a contrast agent for optical imaging into light and, thereby, improving resolution of optical imaging.
In one general aspect, the present disclosure provides a contrast agent for optical imaging, including a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
In another general aspect, the present disclosure provides a contrast agent for optical imaging, including: a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay; and a fluorophore, wherein the energy emitted from the radionuclide is accumulated in the fluorophore and light is emitted from the fluorophore:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
According to a specific embodiment of the present disclosure, the fluorophore may be one or more selected from a group consisting of a quantum dot nanoparticle, Cy3.5, Cy5, Cy5.5, Cy7, indocyanine green (ICG), Cypate, ITCC, NIR820, NIR2, IRDye78, IRDye80, IRDye82, Cresy Violet, Nile Blue, Oxazine 750, Rhodamine800 and Texas red.
In another general aspect, the present disclosure provides a method for acquiring optical images, including injecting the afore-described contrast agent for optical imaging into a living organism.
In another general aspect, the present disclosure provides a contrast agent for trimodality (optical/PET/MR) imaging, including a superparamagnetic nanoparticle labeled with a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
In another general aspect, the present disclosure provides an apparatus for acquiring optical images by detecting light emitted from a contrast agent for optical imaging, including: a chamber accommodating a subject containing the contrast agent for optical imaging; a conversion means provided in the chamber and converting the energy of a charged particle emitted from the contrast agent for optical imaging into light; and a light detection means detecting the light converted by the conversion means.
The terms used in the specification will be described briefly.
Unless specified otherwise, a “contrast agent for optical imaging” refers to a chemical or biological substance which allows, after being injected into a subject, acquisition of optical images by detecting fluorescent or luminescent light outside the subject.
A “multimodality contrast agent” refers to a contrast agent that can be used for different imaging modes, such as a contrast agent that can be used for both nuclear imaging (e.g., PET) and optical imaging or one that can be used for nuclear imaging, optical imaging and magnetic resonance (MR) imaging.
“Autoluminescence” refers to emission of light without energy input from outside.
An “optical/nuclear bimodal imaging contrast agent” refers to a contrast agent that can be used for both nuclear imaging (e.g., PET) and optical imaging.
To perform treatment and diagnosis at the same time means that radiation therapy and monitoring of, e.g., the effect of the radiation therapy by means of noninvasive nuclear or optical imaging are carried out using a single radiopharmaceutical.
The contrast agent for optical imaging of the present disclosure does not require a fluorophore or a luminophore since it uses a radionuclide that emits light by converting the energy of a charged particle emitted during radioactive decay into light energy. Thus, optical images can be acquired while minimizing the change of physicochemical properties of the target molecules. Furthermore, when a fluorophore is attached, light emission may be enhanced without energy input from outside since light is emitted from the fluorophore. Accordingly, it may be widely used to acquire optical images in various applications including clinical studies. Especially, it is very useful in acquiring optical images of a living organism in real time, in a noninvasive manner.
When a superparamagnetic nanoparticle is labeled with a radionuclide satisfying Equation 1, an optical/PET/MR trimodal imaging contrast agent may be prepared simply and in high yield, without attachment of a fluorophore needed for conventional optical imaging.
In addition, the contrast agent for optical imaging of the present disclosure is applicable to commercially available optical imaging instruments to acquire optical images without any modification. The optical imaging using the radionuclide allows noninvasive imaging studies using numerous radiopharmaceuticals developed thus far and less expensive optical imaging instruments without further pretreatment processes, which were possible only with expensive instruments.
And, the apparatus for acquiring optical images of the present disclosure provides better resolution since it includes a conversion means converting the energy of a charged particle emitted from the contrast agent for optical imaging of the present disclosure into light. It also allows optical imaging using the same labeling method as that of nuclear imaging. Although it was difficult to detect the nuclides emitting little or no gamma rays (e.g., β−-emitting nuclides such as 32P) using a radio-TLC scanner, an effect similar to that of radio-TLC scanning may be attained with the optical imaging.
The above and other objects, features and advantages of the present disclosure will become apparent from the following description of certain exemplary embodiments given in conjunction with the accompanying drawings, in which:
a shows a luminescence image of a BALB/c mouse at 1 hour post-injection of [18F]FDG, and
a shows a luminescence image of a 96-well plate containing 300 μL of an aqueous solution of 124I, 18F, 68Ga, 131I and 99mTc at various concentrations,
FIG. 14 shows photographic (A) and luminescence (B) images of a plant (arabidopsis) at 10 minutes after immersion in water (left, control) or in a [32P]phosphoric acid solution. Radiation from selected leaves (red ovals in (A)) with time is shown in (C).
Hereinafter, the embodiments of the present disclosure will be described in detail with reference to accompanying drawings.
As described above, there have been various attempts to combine nuclear imaging and optical imaging having their strong and weak points, and hybrid type imaging probes have been developed using nanoparticles such as quantum dots and near-IR fluorophores. However, in order to obtain both nuclear and optical images using one contrast agent, a hybrid type probe prepared by conjugating a radionuclide for nuclear imaging with a fluorescent or bioluminescent probe for optical imaging is needed. Especially, whereas the nuclear imaging requires only one radioisotope to be introduced to obtain nuclear images, the optical imaging requires that a fluorophore or a luminophore be attached to the radioisotope. However, since most fluorophores or luminophores are bulky metal complexes, aromatic organic compounds or nanoparticles such as quantum dots, they change the physicochemical properties of the target molecules in many cases and, thus, their use in living organisms is restricted.
The inventors of the present disclosure have confirmed that specific radionuclides can emit light even without a fluorophore or a luminophore and that optical images can be acquired by detecting the emitted light. Accordingly, when the specific radionuclides are used, for example, as a contrast agent, optical images can be acquired easily without having to attach a fluorophore. As a result, the optical images can be acquired in real time without changing the physicochemical properties of a substrate.
A contrast agent for optical imaging according to an embodiment of the present disclosure comprises a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
Equation 1 is an equation about the threshold for the emission of Cerenkov radiation. Autoluminescence can occur from the radionuclide when it emits a charged particle having energy with a threshold T satisfying Equation 1.
a shows a luminescence image of a BALB/c mouse bearing luciferase-transfected 4T1 tumour cells taken in luminescence mode at 1 hour after injecting the PET tracer [18F]FDG (18F-analog of glucose, 409 μCi). An intense signal was detected in the brain and brown fat area on the shoulder. The injection route of [18F]FDG (tail) was also clearly visualized. Because there is no external light source in the luminescence imaging mode and no luciferin had been injected before, the light signal is believed to have originated from the injected radiotracer. The luminescence image is also well matched with the expected biodistribution pattern of high [18F]FDG uptake in the brain and brown fat. A different radionuclide, [124I]NaI ion (500 μCi), was injected intraperitoneally into a normal mouse (C3H/HeN) and the luminescence image was measured in an attempt to detect radionuclides by luminescence imaging (
The origin and properties of the luminescence light were characterized systematically using five different radioisotope samples at various activity concentrations and taking luminescence images (
The relationship between the luminescence signal and decay mode of each radionuclide was examined further and the intensity of the luminescence signal depending on radioactivity was quantified by in vitro luminometric assay. Eight different radionuclide samples, which decay in various decay modes, were prepared at consecutively diluted concentrations (
A close examination of the physical properties of the selected radionuclides revealed a good correlation between the luminescence intensity and the energy of particles emitted during decay, such as positrons and electrons, rather than the decay mode, Q-value, and energy of γ-rays emitted from radionuclides (Table 1).
68Ga
32P
124I
18F
131I
64Cu
111In
99mTc
35S
[a]Physical properties of the radionuclides were adapted from MIRD-07.
[b]Relative luminescence intensity was calculated from in vitro luminometer data with respect to the luminescence signal of 131I set to 1.
[c]Relative intensity was calculated from the IVIS 200 data.
Among the five nuclides emitting strong to moderate luminescence signals, three nuclides, 68Ga, 124I and 18F, decayed via β+ decay and emitted positrons with 62 + mean energy (Eβ+mean) of 829, 819 and 250 keV, respectively. On the other hand, 32P and 131I decayed via one hundred percent β− decay and also emitted β− particles with Eβ−mean of 695 and 182 keV, respectively. 64Cu followed both β+ and β− decay modes and emitted both high energy positron (Eβ+mean 278 keV) and electron (Eβ−mean 190 keV). However, three nuclides, 111In, 99mTc and 35S, with weak luminescence signal emitted only low energetic β− particles or very small quantity of β− particles.
To conclude, luminescence occurs during radioactive decay of some radionuclides and optical images can be acquired only with the radionuclides without additional fluorophore or luminophore.
The good correlation between the luminescence intensity and energy of charged particle emitted during decay is reminiscent of Cerenkov radiation. Cerenkov radiation is the light emitted when a charged particle travels with a velocity greater than that of light in a given medium. When a charged particle traveling with a velocity greater than that of light in a medium loses its kinetic energy and slows down to the speed of light, the decreased kinetic energy is turned into light energy. A typical example of Cerenkov radiation is the bluish glow observed around an operating nuclear reactor core. Until now, the applications of Cerenkov radiation have been concerned primarily with cosmic-ray and high-energy physics measurements. Some high energy β-emitting nuclides, such as 32P, were measured using Cerenkov light, which is commonly known as ‘Cerenkov counting’. The threshold for the emission of Cerenkov radiation can be calculated by Equation 1.
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where T is the threshold for the emission of Cerenkov radiation, and n is the refractive index of the medium.
Because water has a refractive index of 1.33, the minimum energy for the emission of Cerenkov radiation is 262 keV when a contrast agent comprising the radionuclide of the present disclosure is injected into a living organism. Therefore, theoretically any radionuclide that emits a positron or β-particle with energy >262 keV during decay can emit Cerenkov radiation and be detected using a high-sensitivity luminescence detector. This explains why high energy electron- and positron-emitting nuclides, such as 68Ga, 32P, 124I, 18F, 131I and 64Cu, emit strong luminescence light while 111In, 99mTc and 35S emit very weak light signals.
The charged particle emitted during the decay of a radionuclide has a varying energy distribution. To take 18F as an example, the emitted charged particle has a mean energy of 250 keV. Since the maximum emission energy is 634 keV, the emitted charged particle has a continuous energy distribution between 0 and 634 keV. From the requirement of Equation 1, the minimum energy required for radiation in air (refractive index=1.0003) is 20355 keV. Accordingly, light is not emitted for the radionuclide, since the maximum energy is only 634 keV. However, if the medium is water (refractive index=1.33), the minimum energy required for radiation is smaller than in air, with 262 keV. Thus, among the emitted charged particles, one having an energy of 262 keV or higher can emit light. In other words, when a charged particle traveling with a speed greater than that of light loses its kinetic energy and slows down to the speed of light, the decreased kinetic energy is turned into light energy. If the medium is glass (refractive index=1.52) with a refractive index higher than that of water, a charged particle having an energy 168 keV or higher can emit light. In this example, although luminescence occurs both in water or glass, glass having a higher refractive index requires less energy for radiation than water having a lower refractive index. As a result, the energy emitted from charged particles with a broader energy distribution can be converted into light, and thus light emission is enhanced.
Specifically, as seen from
However, the medium is not limited to glass. Depending on the type and state of the subject, air (refractive index=1.0003), water (refractive index=1.33) or other materials with high refractive indices (e.g., glass, high refractive plastic, etc.) may be used as a medium to enhance the intensity of Cerenkov emission from the radionuclide. By using a material with a high refractive index as the medium, the minimum energy required for the emission of Cerenkov light can be reduced and, hence, the light emission can be improved. Moreover, by injecting a substance with a high refractive index into the subject, more distinct optical images can be obtained since the light emission is enhanced.
The charged particle emitted from the radionuclide may be electron, positron or a particle depending on the mode of radioactive decay.
The intensity of a photon emitted by Cerenkov radiation is proportional to 1/λ2 and increases as the energy of the charged particle increases beyond the Cerenkov threshold. This explains further why the relative intensity of 68Ga and 32P is higher than that of 124I, 18F, 131I and 64Cu. Although the mean positron energy of 68Ga is similar to 124I (829 vs. 819 keV), the relative intensity of 68Ga is more than 4 times higher than that of 124I because the branching ratio to the positron decay of 68Ga is approximately 4 times larger than that of 124I (89 vs. 23%). The radionuclide satisfying Equation 1 is likely to be a radionuclide following β+, β− or electron capture decay. In this regard, the radionuclide that can be employed in the present disclosure may be a radionuclide that decays via β+ decay such as 10C, 11C, 13O, 14O, 15O, 12N, 13N, 15F, 17F, 18F, 32Cl, 33Cl, 34Cl, 43Sc, 44Sc, 45Ti, 51Mn, 52Mn, 52Fe, 53Fe, 55Co, 56Co, 58Co, 61Cu, 62Cu, 62Zn, 63Zn, 64Cu, 65Zn, 66Ga, 66Ge, 67Ge, 68Ga, 69Ge, 69As, 70As, 71Se, 71Se, 71As, 72As 73Se, 74Kr, 74Br, 75Br, 76Br, 77Br, 77Kr, 78Br, 78Rb, 79Rb, 79Kr, 81Rb, 82Rb, 84Rb, 84Zr, 85Y, 86Y, 87Y, 87Zr, 88Y, 89Zr, 92Tc, 93Tc, 94Tc, 95Tc, 95Ru, 95Rh, 96Rh, 97Rh, 98Rh, 99Rh, 100Rh, 101Ag, 102Ag, 102Rh, 103Ag, 104Ag, 105Ag, 106Ag, 108In, 109In, 110In, 115Sb, 116Sb, 117Sb, 115Te, 116Te, 117Te, 117I, 118I, 118Xe, 119Xe, 119I, 119Te, 120I, 120Xe, 121Xe, 121I, 122I, 123Xe, 124I, 126I, 128I, 129La, 130La, 131La, 132La, 133La, 135La, 136La, 140Sm, 141Sm, 142Sm, 144Gd, 145Gd, 145Eu, 146Gd, 146Eu, 147Eu, 147Gd, 148Eu, 150Eu, 190Au, 191Au 192Au, 193Au, 193Tl, 194Au, 195Tl, 196Tl, 197Tl, 198Tl, 200Tl, 200Bi, 202Bi, 203Bi, 205Bi or 206Bi, a radionuclide that decays via β− decay such as 3H, 14C, 35S, 32P, 131I, 59Fe, 60Co, 67Cu, 89Sr, 90Sr, 90Y, 99Mo, 133Xe, 137Cs, 153Sm, 177Lu or 186Re, or a radionuclide that decays via electron capture such as 111In, 123I, 125I, 201Tl, 67Ga, 51Cr, 57Co, 58Co, 62Zn or 82Sr. Most specifically, it may be 18F, 11C, 13N, 15O, 60Cu, 64Cu, 67Cu, 124I, 68Ga, 52Fe, 58Co, 3H, 14C, 35S, 32P, 131I, 59Fe, 60Co, 89Sr, 90Sr, 90Y, 99Mo, 133Xe, 137Cs, 153Sm, 177Lu, 186Re 123I, 125I, 201Tl or 67Ga, but is not limited thereto. Since other radionuclides that do not decay via β+, β− or electron capture can also emit light, they may also be used as the radionuclide of the present disclosure if Equation 1 is satisfied.
Thus, it is thought that Cerenkov radiation is the main contribution of the luminescence occurring during radioactive decay of the radionuclide according to the present disclosure, and it may be utilized in a contrast agent and for optical imaging without having to attach a fluorophore or a luminophore. Specifically, NIH3T6.7 tumours implanted in a nude mouse were clearly visualized by 124I-labeled Herceptin (trastuzumab) antibody in luminescence imaging (
The application of Cerenkov imaging can be extended easily to multimodality imaging. A single imaging probe radiolabeled with an appropriate radioisotope can be used as an optical and nuclear dual imaging probe on account of the intrinsic dual imaging potential of the radionuclide emitting high energetic particles during decay.
In a specific embodiment, the present disclosure provides a contrast agent for trimodality (optical/PET/MR) imaging, comprising a superparamagnetic nanoparticle labeled with a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
Multimodality imaging has been considered as an important diagnosis tool during the past decades in basic biomedical researches and clinical studies. Nevertheless, the currently available imaging modalities, including optical, X-ray, nuclear, magnetic resonance (MR) and ultrasound imaging, cannot provide all the information needed for elucidating the complicated biological events in living organisms. Each imaging modality has its own advantages and disadvantages in terms of sensitivity, temporal and spatial resolution, tissue penetration, quantification, instrument cost, availability, radiation exposure, functional information, and so forth. Therefore, the combination of two or more imaging modalities often provide the combined advantages of each while compensating for the weak points of each modality. Among the various multimodality imaging agents that have been developed in this context to date, optical/nuclear, optical/MR and nuclear/MR dual imaging probes have attracted special interest because of the high degree of complementarity among these three optical, nuclear and MR imaging modalities. Optical imaging techniques such as fluorescence and bioluminescence imaging can be obtained in short scanning time (<1 min) with high sensitivity using relatively cheap imaging instruments. But, they suffer from a low tissue penetration problem, leading to poor quantification and limitation in use to imaging of small animals. Nuclear imaging such as positron emission tomography (PET) has the advantages of high sensitivity and accurate quantification, even for humans, but its spatial resolution is poor, being limited to the range of several millimeters. On the other hand, MR imaging (MRI) can provide excellent anatomical information in the sub-millimeter range, especially for soft tissue, but its sensitivity is far inferior to that of optical and nuclear imaging. Nuclear imaging and MRI both provide three-dimensional (3D) tomographic images for clinical use, but the high cost of imaging scanners has limited access by basic researchers.
Therefore, the development of an optical/PET/MR imaging probe will provide numerous benefits. Using a single hybrid probe, the biological events of interest in living organisms can be quickly screened with high sensitivity using an easily accessible optical imaging instrument, so that, subsequently, only the selected subjects are subjected to PET and MR imaging for further accurate visualization of the injected probe supported by detailed 3D anatomical information. In many cases, however, integrating individual imaging probes into a single hybrid system to achieve such multimodality imaging requires multi-step synthesis and purification processes, which often result in significant loss of optical, PET and MR signals. This explains why only a few examples of triple-modality imaging probes have been reported so far. Therefore, a facile method for the preparation of a triple-modality probe is needed.
The triple-modality imaging probe of the present disclosure can be constructed by conjugating an appropriate PET radionuclide that is able to generate Cerenkov light to a magnetic probe. Unlike the existing methods that combine three different individual probes to generate triple-modality images, the probe can be prepared by using only two different components: the PET and MR imaging agents.
In order to evaluate the feasibility of using Cerenkov light as a new optical imaging modality, the inventors selected a 124I radioisotope among the many PET radionuclides due to its sufficiently long half-life (4.2 days) for probe preparation, and more importantly, its emission of high-energy positrons during its decay with a β+ mean energy (Eβ+mean) of 819 keV, leading to strong Cerenkov radiation. On the other hand, they selected a thermally cross-linked, superparamagnetic iron oxide nanoparticle (TCL-SPION) as an MRI contrast agent due to its high stability in vivo and excellent anti-biofouling property owing to the cross-linked polymer coating layers containing polyethylene glycol (PEG) on the magnetite core.
To evaluate the sensitivity of the Cerenkov luminescence imaging, a series of solutions (300 μL) containing 124I and TCL-SPION were imaged by optical, microPET and MR imaging (
The spatial resolution of Cerenkov imaging was studied using a Derenzo phantom having holes of different sizes filled with radioactive sources. A Derenzo phantom containing 32 μCi/mL 124I solution was imaged by luminescence and microPET (
The great potential of Cerenkov radiation as a new optical imaging modality and, hence, the intrinsic optical/PET dual-imaging property of some PET radionuclides provide a simpler method of preparing a hybrid optical/PET/MR imaging nanoprobe by conjugating an appropriate PET radionuclide (124I) to an MRI probe (TCL-SPION). A schematic illustration for the preparation of the 124I-labeled TCL-SPION is shown in
The radiolabeling of the tyramine-conjugated TCL-SPIONs with 124I was easily accomplished using commercially available Iodo-Beads (Pierce Biochemical Co., USA). Radioactive iodine offers the significant two advantages of well-established radioiodination and a high radiolabeling yield that can easily be achieved using one of several commercially available labeling procedures. In the treatment of [124I]NaI with Iodo-Beads, cationic iodine species (ICl) are formed and these reactive electrophiles form bonds with the phenol group at the ortho position (
To examine the potential of the 124I-labeled TCL-SPIONs as a triple-modality imaging probe in vivo, the inventors performed sentinel lymph node imaging. Accurate imaging of the sentinel lymph node, especially the differentiation of a metastasized lymph node against tumor-free sentinel lymph nodes, is crucial, because many tumors spread out to other organs via the lymphatic vessels, and the surgical dissection area is determined based on the location of the metastasized sentinel lymph nodes. The tyramine-conjugated TCL-SPIONs have a hydrodynamic size of about 40 nm, which is included in the ideal size range for a lymph node imaging agent (5-50 nm), and its high in vivo stability and anti-biofouling properties further increase its suitability for lymph node imaging.
A tumor metastasis model was prepared by injecting the mouse breast cancer cell line 4T1 into the left shoulder of normal BALB/c mice. About ten days later, the 124I-labeled TCL-SPIONs (38 μg, 20 μCi per injection) were injected into both front paws of a 4T1 tumor-bearing BALB/c mouse. Luminescence, microPET and MR imaging were then performed to see if the metastasized lymph node could be differentiated from a tumor-free sentinel lymph node (
Although the superparamagnetic iron oxide-based nanoparticle (TCL-SPION) was described as an example of the superparamagnetic nanoparticle that can be used in the present disclosure, other various known superparamagnetic nanoparticles may also be used. The superparamagnetic nanoparticle may be modified partly or wholly on its surface in order to make labeling with the radionuclide satisfying Equation 1 easier.
In conclusion, Cerenkov imaging showed great potential as a new optical imaging modality. Thanks to the intrinsic optical/PET dual-imaging properties of 124I, the extra step of conjugating a fluorescent dye that is needed for optical imaging could be omitted, resulting in a simpler but higher yielding preparation of a hybrid nanoprobe for triple-modality imaging. The lymph node metastasis was screened with high sensitivity within a few minutes using an easily accessible optical imager, but the final diagnosis was based on accurate 3D visualization of accumulated nanoprobes with detailed anatomical information provided by PET and MR imaging. This facile method for the preparation of multimodality imaging probes based on Cerenkov imaging has a promising potential for application in many other biomedical and clinical research fields such as cell trafficking, tumor imaging and theragnosis.
According to another embodiment of the present disclosure, it is possible to monitor pure β+- or β−-emitting radionuclides by non-invasive optical imaging using the contrast agent of the present disclosure. Thus far, due to the lack of γ-ray emission during decay, pure β− emitters, such as 32P and 90Y, could be radioassayed using only invasive methods, such as liquid scintillation and Cerenkov counting, in which the measurements were performed with extracts. As an example, the phosphate uptake pattern in a plant (arabidopsis) was imaged in real time in luminescence mode, using 32P-labeled phosphoric acid [32P]phosphoric acid (
In another embodiment, the present disclosure provides a contrast agent for optical imaging comprising: a radionuclide which emits a charged particle having energy with a threshold T satisfying Equation 1 during radioactive decay; and a fluorophore, wherein the energy emitted from the radionuclide is accumulated in the fluorophore and light is emitted from the fluorophore:
T (keV)=511[1/(1−1/n2)1/2−1] [Equation 1]
where n is the refractive index of a medium.
Descriptions about Equation 1 and the radionuclide satisfying the equation will be omitted, since they are the same as described above. The existing imaging agent requires the attachment of, for example, a fluorophore to a substrate for optical imaging even if it already includes a radionuclide. In that case, the procedure of irradiating light in the UV or visible region was needed to excite the fluorophore prior to optical imaging. In contrast, the radionuclide according to the present disclosure satisfying Equation 1 can autoluminesce without external energy input since the fluorophore can absorb the light emitted from the radionuclide and reaches the excited state. Specifically, as can be seen from
Examples of the fluorophore that can be used in the present disclosure may include fluorescein, rhodamine, Lucifer yellow, B-phycoerythrin, 9-acridine isothiocyanate, Lucifer yellow VS, 4-acetamido-4′-isothiocyanatostilbene 2,2′-disulfonic acid, 7-diethylamino-3-(4′-isothiocyanatophenyl)-4-methylcoumarin, succinimidylpyrene butyrate, 4-acetamido-4′-isothiocyanatostilbene 2,2′-disulfonic acid derivatives, LC™-Red 640, LC™-Red 705, Cy5, Cy5 lissamine, isothiocyanate, erythrosin isothiocyanate, diethylenetriamine pentaacetate, 1-dimethylaminonaphthyl 5-sulfonate, 1-anilino-8-naphthalene sulfonate, 2-p-touidinyl-6-naphthalene sulfonate, 3-phenyl-7-isocyanatocoumarin, 9-isothiocyanatoacridine, acridine orange, 9-(isothio-(2-benzoxazolyl)phenyl)maleimide, benzoxadiazole, stilbene, pyrene, fluorophore-containing silica, a group II/IV semiconductor quantum dot, a group III/V semiconductor quantum dot, a group IV semiconductor quantum dot, or a bi- or multi-hybrid structure. Specifically, the fluorophore may be one or more selected from a group consisting of a quantum dot nanoparticle, Cy3.5, Cy5, Cy5.5, Cy7, indocyanine green (ICG), Cypate, ITCC, NIR820, NIR2, IRDye78, IRDye80, IRDye82, cresyl violet, Nile blue, Oxazine 750, Rhodamine 800, lanthanides and Texas red. Most specifically, the fluorophore may be a quantum dot nanoparticle of a group II-VI or group III-V compound. In this case, the quantum dot nanoparticle may be one or more selected from a group consisting of CdSe, CdSe/ZnS, CdTe/CdS, CdTe/CdTe, ZnSe/ZnS, ZnTe/ZnSe, PbSe, PbS, InAs, InP, InGaP, InGaP/ZnS and HgTe. The molar ratio of the radionuclide to the fluorophore may be from 1:1000 to 1000:1, but the present disclosure is not limited thereto.
Further, the present disclosure provides a contrast agent for optical imaging comprising: the autoluminescent radionuclide of the present disclosure; and at least one of a bioactive substance or a chemical active substance. Specifically, the bioactive substance or the chemical active substance may be selective for or specific to a targeted organ or tissue, and may further comprise the fluorophore described above.
The bioactive substance or chemical active substance may be a hormone, an amino acid, a peptide, a peptidomimetic, a protein, a nucleoside, a nucleotide, a nucleic acid, an enzyme, a carbohydrate, a glycomimetic, a lipid, albumin, a monoclonal or polyclonal antibody, a receptor, an encapsulation compound such as cyclodextrin, or a receptor binding molecule. Specific examples of the bioactive substance or chemical active substance include: steroid hormones for the treatment of breast and prostate lesions; somatostatin, bombesin, cholecystokinin (CCK) and neurotensin receptor binding molecules for the treatment of neuroendocrine tumors; CCK receptor binding molecules for the treatment of lung cancer; ST receptor and carcinoembryonic antigen (CEA) binding molecules for the treatment of colorectal cancer; dihydroxyindolecarboxylic acid and other melanin producing biosynthetic intermediates for the treatment of melanoma; integrin receptor and atherosclerotic plaque binding molecules for the treatment of vascular diseases; and amyloid plaque binding molecules for the treatment of brain lesions. Other examples of the bioactive substance or chemical active substance include synthetic polymers, such as polyaminoacids, polyols, polyamines, polyacids, oligonucleotides, aborols, dendrimers, and aptamers.
In an embodiment of the present disclosure, the bioactive substance or chemical active substance may be selected from nanoparticles, antibodies (e.g., NeutroSpect®, Zevalin® and Herceptin®)), proteins (e.g., TCII, HSA, annexin and Hb), peptides (e.g., octreotide, bombesin, neurotensin and angiotensin), nitrogen-containing simple or complex carbohydrates (e.g., glucosamine and glucose), nitrogen-containing vitamins (e.g., vitamin A, B1, B2, B12, C, D2, D3, E, H and K), nitrogen-containing hormones (e.g., estradiol, progesterone and testosterone), nitrogen-containing active pharmaceuticals (e.g., celecoxib or other nitrogen-containing NSAIDs, AMD3100, CXCR4 and CCR5 antagonists) or nitrogen-containing steroids.
As described above, the contrast agent according to the present disclosure may comprise various bioactive substances or chemical active substances. To increase, for example, specificity for a particular target tissue, organ receptor or other biologically expressed composition, multiple bioactive substances or chemical active substances may be utilized. In such instances, the bioactive substances or chemical active substances may be the same or different. For example, a single contrast agent may comprise multiple antibodies or antibody fragments, which are directed against desired antigens or haptens. Typically, the antibodies used in the contrast agent may be monoclonal antibodies or antibody fragments that are directed against desired antigens or haptens. Thus, for example, the contrast agent may comprise two or more monoclonal antibodies having specificity for a desired epitope and thereby increasing concentration of the contrast agent at the desired site. Similarly and independently, the contrast agent may comprise two or more different bioactive substances or chemical active substances each of which is targeted to a different site of the same target tissue or organ. By utilizing multiple bioactive substances or chemical active substances in this manner, the contrast agent may be advantageously concentrated at several areas of the target tissue or organ, thereby potentially increasing the effectiveness of therapeutic treatment. Further, the contrast agent may have a certain ratio of the bioactive substance or chemical active substance designed to concentrate the contrast agent at a target tissue or organ such that the desired therapeutic and/or diagnostic results may be optimally achieves.
The contrast agent for optical imaging of the present disclosure is able to perform radiation therapy and diagnosis at the same time. By injecting a contrast agent for optical imaging comprising a radionuclide emitting β− ray or α particles into a living organism, optical images may be obtained through luminescence resulting from the electrons or a particles emitted with sufficient energy during decay while treating a tumor or a disease, which may be utilized to monitor, for example, the therapeutic effect.
In another embodiment, the present disclosure provides a pharmaceutical composition comprising the contrast agent of the present disclosure and a pharmaceutically acceptable carrier. Specifically, the pharmaceutical composition according to the present disclosure may comprise a contrast agent, which forms a complex with a metal, dispersed in a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier (also known as excipient, vehicle, adjuvant or diluent in the related art) is usually a pharmaceutically inactive substance, providing adequate hardness or shape to the composition without negatively affecting the therapeutic and/or diagnostic effect of the contrast agent. In general, the carrier is regarded as “pharmaceutically or pharmacologically acceptable” unless it leads to adverse reactions, allergic reactions or other undesired reactions when administered to a mammal, especially human.
The selection of the pharmaceutically acceptable carrier is likely to be affected at least in part by the targeted administration route. In general, the pharmaceutical composition according to the present disclosure may be formulated to be applicable to any administration route allowing access to the target tissue. For example, adequate administration routes may include oral, parenteral (e.g., intravenous, intraarterial, subcutaneous, rectal, intramuscular, intraorbital, intracapsular, intramedullary, intraperitoneal or intrasternal), topical (e.g., nasal, transdermal or intraocular), intravesical, intrathecal, intraabdominal, intrapulmonary, intralymphatic, intracavitary, intravaginal, transurethral, intradermal, intratympanic, intrabreast, buccal, in situ, endotracheal, intraleisonal, endoscopic, transmucosal or sublingual routes.
Pharmaceutically acceptable solvents that can be used in the present disclosure are known to those skilled in the art. For example, The Chemotherapy Source Book (Williams & Wilkens Publishing) may be referred to.
The administration dosage of the contrast agent and/or the pharmaceutical composition according to the present disclosure may be determined easily by those skilled in diagnosis or treatment of diseases. The administration dosage of the contrast agent will be different depending on the age, sex, general health and body weight of the subject, the kind of treatment accompanied, the number of treatments, if any, and the desired effect. Given a specific mode of administration, the actual amount of the delivered contrast agent as well as the administration scheme needed to attain the desired effect will be different, at least in part, depending on the bioavailability of the contrast agent in a living organism, hindrance to treatment or diagnosis, administration dosage for the desired treatment or diagnosis, and other factors apparent to those skilled in the art. The administration dosage for an animal, especially human, should be sufficient enough to exhibit a desired therapeutic or diagnostic effect over an appropriate period.
The present disclosure provides a contrast agent having an adequate radioactivity. In general, it is desired that a radioactive complex is formed in a solution having a radioactivity concentration of about 0.01-1000 mCi/mL. In general, a unit administration dosage may have a radioactivity of about 0.01-1000 mCi, specifically about 1-300 mCi. The volume of a solution injected as a unit administration dosage is about 0.01-20 mL.
A linker may be used to attach the radionuclide with the bioactive substance or chemical active substance. The linker may be a compound represented by Chemical Formula 1, but is not limited thereto:
where R, R1 and R2 are independently C1-10 alkyl, C1-10 alkenyl, C1-10 alkynyl, C1-10 aryl, C1-10 arylalkyl or C1-10 heteroaryl, and n is independently an integer from 1 to 20.
As described earlier, the contrast agent for optical imaging of the present disclosure may be used in existing apparatuses for acquiring optical images. More desirably, the present disclosure provides an apparatus for acquiring optical images by detecting light emitted from the contrast agent for optical imaging according to the present disclosure in order to more clearly visualize Cerenkov light, comprising: a chamber accommodating a subject containing the contrast agent for optical imaging; a conversion means provided in the chamber and converting the energy of a charged particle emitted from the contrast agent for optical imaging into light; and a light detection means detecting the light converted by the conversion means. To describe specifically the features of the apparatus referring to
Specifically, the subject may contain the contrast agent for optical imaging of the present disclosure therein. The subject may be one commonly used to acquire an optical image without particular limitation. Specifically, it may be a living organism, a tissue, a TLC plate, a gel, a sample, and so forth.
The conversion means 130 serves to convert the energy of a charged particle emitted from the contrast agent for optical imaging included in the subject into light. Specifically, it is the medium of Equation 1. The conversion means 130 may be provided at any position where the light emitted from the subject can reach, without particular limitation. Specifically, it may be provided in contact with or very close to the subject. The conversion means 130 may also be provided on the supporting means 140. As described earlier referring to Equation 1, since the intensity of Cerenkov radiation is proportional to the refractive index of the medium, the higher the refractive index of the conversion means 130, the easier it is to convert the energy of the charged particle emitted from the contrast agent for optical imaging included in the subject into Cerenkov light. Specifically, if the subject is a simple sample, the conversion means 130 may have a refractive index higher than that of air (1.0003). If the subject is a living organism (e.g., mouse or human), the conversion means 130 may have a refractive index higher than that of water (1.33), more specifically, 1.50 or higher. Although glass having a refractive index of 1.52 was used as the conversion means in the examples related to
Specifically, the conversion means 130 may be in the form of a sheet. The thickness may be 0.1-20 mm, but is not limited thereto. The number of the conversion means 130 may be one or more. The conversion means 130 may be configured to be separable as occasion demands and may be configured to be on/off controllable. In addition, it may be configured to have a variable refractive index using, for example, a filter. Also, the apparatus may be configured to capture the light emitted from the subject directly without using the conversion means.
The light detection means 110 serves to detect the light converted by the conversion means 130 and convert it into an optical image, and may comprise a photographing or imaging device commonly used in an apparatus for acquiring optical images. Specifically, the light detection means 110 may comprise a lens and/or a filter 120 through which the light converted by the conversion means 130 passes and a charge-coupled device (CCD) camera 111 or a photomultiplier tube (PMT) for converting the light into an optical imaging. However, without being limited thereto, the light detection means commonly used in an apparatus for acquiring optical images may be used without limitation.
The chamber 160 may be one through which light cannot penetrate. The chamber may be made of a radiation-shielding lead or tungsten material, or may be equipped with a shielding means. However, the present disclosure is not limited thereto. The apparatus for acquiring optical images of the present disclosure may be used as an apparatus for luminescence imaging, fluorescence imaging, bioluminescence imaging, radioluminescence imaging or a combination thereof. However, without being limited thereto, it may be utilized without limitation as long as the principle of the present disclosure is applicable.
Further, in case the subject is an animal, an anesthetic means 150 for anesthetizing may be included in the apparatus for acquiring optical images of the present disclosure.
Operation of the apparatus for acquiring optical images according to the afore-described embodiment of the present disclosure will be described. First, a subject is positioned on the supporting means 140. The subject contains (through injection) the contrast agent for optical imaging of the present disclosure. Thus, a charged particle (e.g., electron or positron) having energy (kinetic energy) is emitted from the contrast agent for optical imaging included in the subject. If the emitted charged particle satisfies Equation 1, it has a velocity greater than that of speed of light. Then, the charged particle is decelerated to the speed of light while passing through the conversion means 130, and the corresponding energy difference is emitted as light. Specifically, the conversion means 130 may be made of a material having a high refractive index in order to reduce the minimum energy T required to emit Cerenkov light. The light converted by the conversion means 130 is detected by the light detection means 110 to construct an optical image.
An apparatus for acquiring optical images for medical use according to another embodiment of the present disclosure will be described referring to
Specifically, the apparatus 200 for acquiring optical images for medical use may comprise the supporting means 240, the conversion means 230 and the light detection means 220 inside the chamber 210. At least one of the supporting means 240 and the light detection means 220 may be configured to be freely movable relative to each other by providing a position control means. The position control means may be any one that can move the supporting means 240 or the light detection means 220 upward, downward, leftward and rightward. Accordingly, the subject positioned on the supporting means 240 may be examined wholly by moving the supporting means 240 or the light detection means 220. Also, the conversion means 230 may be moved together with the light detection means 220 or the supporting means 240.
The light detection means 220 may be brought in close proximity to the desired site (e.g., thyroid) of the subject using the position control means so as to acquire optical images. The chamber 210 may be one through which light cannot pass. The chamber itself and/or the light detection means 220 may be shielded from radiation. The supporting means 240 may have any shape as long as it is capable of supporting, for example, human, including bed or chair shapes.
According to another embodiment of the present disclosure, the apparatus for acquiring optical images of the present disclosure may be an endoscope. Specifically, referring to
The endoscope 300 may be inserted into a living organism. The living organism has the contrast agent for optical imaging of the present disclosure injected therein. Since the living organism is light-shielded in itself, an additional means to block light penetration into the endoscope 300 is not necessary. Differently from the apparatus for acquiring optical images according to the afore-described embodiment, the endoscope 300 comprises the light source 320 for illuminating the inside of the living organism. The light source 320 may be a lamp. It may be provided at a leading end portion of the endoscope 300, but its location is not limited thereto. And, the conversion means 330 for converting the energy of the charged particle into light in the living organism may be provided either in front of the light source 320 or between the light source 320 and the light detection means 360. Most specifically, it may be provided at the leading end portion of the endoscope, i.e. in front of the light source 320, so as to convert the energy of the charged particle emitted from the contrast agent for optical imaging injected into the living organism at a short distance. A body 310 of the endoscope 300 has the same configuration as the commonly used endoscope. It may include a means (e.g., optical fiber) to transmit the light converted by the conversion means 330 to the light detection means 360.
The principle of converting the energy of the charged particle emitted from the contrast agent for optical imaging, which is contained in the subject, into light using the conversion means 330 and then converting the converted light into an optical image using the light detection means 360 is the same as that described in the above embodiment.
Now, a radionuclide detection apparatus 400 according to another embodiment of the present disclosure will be described referring to
The radionuclide detection apparatus according to this embodiment is the same as the apparatus for acquiring optical images according to the above-described embodiments in basic configuration and operation principle. Hereunder, only the difference from the afore-described embodiments will be described.
The tube 450 may have a diameter of 0.1-10 mm, although not being limited thereto. A fluid passes inside the tube 450. The fluid contains the subject labeled with the contrast agent for optical imaging of the present disclosure. The subject may be any subject that can be subjected to high performance liquid chromatography (HPLC). Specifically it may be antibody, protein, antigen, peptide, nucleic acid, enzyme, cell, carbohydrate, vitamin, hormone, nanoparticle, inorganic support, polymer, single molecule or drug, but is not limited thereto.
The tube 450 penetrates the chamber 440. The tube 450 may have a light transmitting portion 460, so that the light emitted from the subject can be transmitted through the chamber 440. The light transmitting portion 460 may be made of any transparent material allowing penetration of light, without particular limitation. The light transmitting portion 460 may be formed at the position facing the conversion means 430.
The conversion means 430 converts the energy of the charged particle passing through the light transmitting portion 460 into light. The light converted by the conversion means 430 is then converted into an optical image by the light detection means 410.
Now, operation of the radionuclide detection apparatus according to this embodiment will be described. The fluid containing the subject flows in the tube 450. The tube 450 penetrates the chamber 440. The charged particle emitted from the radionuclide labeled at the subject passes through the light transmitting portion 460. Then, the energy of the charged particle is converted into light by the conversion means 430. The converted light is then converted into an optical signal by the light detection means 410. The light transmitting portion 460 may also perform the photoconversion instead of the conversion means 430. Specifically, in another embodiment, the light transmitting portion 460 may be made of a material with a high refractive index (e.g., glass or transparent plastic), so that it may perform the photoconversion even without the conversion means 430.
Unlike the existing apparatus for acquiring optical images, the apparatus for acquiring optical images and the radionuclide detection apparatus of the present disclosure may comprise the conversion means capable of converting the energy of the charged particle emitted from the radionuclide included in the subject into Cerenkov light and/or may comprise the radiation shielding means for protecting the chamber and/or the light detection means from the radionuclide.
The conversion means may be made of a material having a high refractive index. The apparatus for acquiring optical images and the radionuclide detection apparatus may be utilized widely as an apparatus for acquiring optical images for medical or non-medical use, as a radio-detector, for measurement of surface radioactive contamination, as a radiation detector for HPLC, and so forth.
The examples and experiments will now be described. The following examples and experiments are for illustrative purposes only and not intended to limit the scope of the present disclosure.
A BALB/c mouse with a luciferase-transfected 4T1 tumor was injected with 409 mCi of [18F]FDG via the tail vein. One hour after the injection, the mouse was scanned for 3 minutes in the prone position using IVIS 200.
500 mCi of 124I was injected intraperitoneally into a normal mouse (C3H/HeN, Orient Bio Inc., Korea). 40 minutes after the injection, a luminescence image was obtained by scanning the mouse for 1 minute in the lying position.
A 96-well plate filled with each of the successively diluted solutions (300 mL) of [124I]NaI, [18F]FDG, [68Ga]GaCl3, [131I]NaI and [99mTc]TcO4− was scanned for 1 minute using IVIS 200 (
2.0, 1.08, 1.84, 3.06, 1.08, 2.20, 2.42 and 3.0 mCi/mL [32P]phosphoric acid, [124I]NaI, [18F]FDG, [131I]NaI, [64Cu]CuCl2, 111[In]InCl3, [99mTc]TcO4− and [35S]methionine, respectively, were diluted successively one third to prepare 10-11 solutions for each radionuclide. A 96-well plate containing 300 mL of a solution of each radionuclide at different concentrations was scanned for 30 seconds at 470 nm using a bench top luminometer (SpectraMax L, MDS Analytical Technologies, USA). The result is shown in
A similar relationship between the luminescence intensity and radioisotope species was observed. In general, all the radionuclides showed increase in luminescence intensity with increasing radiation activity. However, three isotopes, 111In, 99mTc and 35S, showed much slower increase in light signal compared to the other five nuclides, 32P, 124I, 18F, 131I and 64Cu. In particular, 35S did not show any noticeable increase in luminescence even at the highest activity. Whereas the nuclides emitting high-energy electrons and positrons, 68Ga, 32P, 124I, 18F, 131I and 64Cu, showed strong signals, 111In, 99mTc and 35S showed weak signals.
Therefore, it was confirmed that the luminescence originates from the radionuclides. Also, it was shown that the luminescence of the radionuclides is highly dependent on their decay mode. All the luminescence signals could be blocked completely by covering the well containing the radionuclide with black paper, which suggests that the detected signal originates from the light emitted from the radionuclide, not from any interaction between the charged particle or gamma ray and the detector.
A small quantity of Herceptin (trastuzumab) was radiolabeled with 124I using Iodo-Beads (Pierce Biochemical Co., USA) according to the manufacturer's instructions. To put briefly, the beads were washed with PBS (pH 7.2) and dried on filter paper at room temperature. The washed beads were put in a [124I]NaI (37 MBq) solution in 100 μL of PBS. After shaking for 5 minutes, the [124I]NaI solution containing Iodo-Beads was mixed with an aqueous solution of Herceptin (30 μg, 30 μL of distilled water). Labeling was performed for 15 minutes at room temperature while gently shaking. The reaction was terminated by removing the beads from the reaction tube. The labeling yield was monitored on a silica plate using acetone as a developing solvent, using a radio-TLC imaging scanner (Bioscan Inc., Washington D.C., USA). Unlabeled free 124I ions were removed using a centrifugal filter (Microcon YM 50, Millipore Inc., USA), and the purified 124I-labeled Herceptin (3.29 MBq) was injected into a nude mouse bearing a NIH3T6.7 tumor on the shoulder and flank. The mouse was scanned for 20 min using a microPET at 2 days after the injection, which was immediately followed by luminescence imaging (scan time=1 min).
Immediately after the in vivo imaging, internal organs as well as tumors were dissected out, and luminescence and microPET images were obtained with scan times of 1 minute and 20 minutes, respectively. Relative intensity of each organ in the luminescence and microPET images was calculated by quantification of ROI drawn on each excised organ.
Herceptin was easily radioiodinated with 124I (3.29 MBq) using the well-established Iodo-Beads method, by which an oxidized I+ ion reacts with the tyrosine residue of the antibody. The radiolabeling yield was 78% and the radiochemical purity was increased to 92% after purification using the centrifugal filter. The NIH3T6.7 tumours implanted in the nude mouse were clearly visualized by 124I-labeled Herceptin antibody in luminescence imaging (
Ex vivo optical and microPET images of the dissected organs and tumors showed different radiation uptake patterns (
(1) Synthesis of tyramine-conjugated poly(TMSMA-r-PEGMA-r-NAS)
Poly(TMSMA-r-PEGMA-r-NAS) was synthesized according to the previously reported method. Specifically, as illustrated in
(2) Synthesis of Tyramine-Conjugated TCL-SPION
FeCl3.6H2O (0.5 g, 1.85 mmol) and FeCl2.4H2O were dissolved in deionized water (30 mL) from which gas had been removed by blowing nitrogen for 30 minutes. Then, 7.5 mL of NH4OH (˜28% water) was added to the solution under nitrogen atmosphere while strongly stirring. 30 minutes later, an external magnetic field was applied. The resulting precipitate was washed with deionized water to collect the black particles. After discarding the supernatant, 250 mg of the tyramine-conjugated poly(TMSMA-r-PEGMA-r-NAS) in 30 mL of deionized water was added, and the mixture solution was stirred for 2 hours. After purification to remove the remaining unreacted polymer, ultrasonic pulverization was performed for 30 minutes in 20 mL of deionized water using a VCX-500 ultrasonic processor (Sonics & Materials, Inc., Newtown, Conn.). Then, after applying an external magnetic field overnight, the precipitated particles were removed by centrifugation for 10 minutes at 10,000 rpm. The supernatant was diluted with deionized water and then heated for 1.5 hours at 80° C. in order to form cross-linkages between polymer rings on the particle surface. The resulting thermally cross-linked tyramine-conjugated superparamagnetic iron oxide nanoparticle (TCL-SPION) was concentrated for animal tests by filtration (Amicon Co., USA). The magnetite core was analyzed by tunneling electron microscopy (TEM) using a TECNAI F20 electron microscope (Philips Lectronic Instruments Corp., Mahwah, N.J.) operating at 200 kV. The hydrodynamic particle size of the tyramine-conjugated TCL-SPION was measured as 39.8±8.3 nm using a Photal ELS-8000 electrophoretic light scattering apparatus (Otsuka Electronics Co., Japan). The TEM analysis revealed that the magnetite core size was between 4 and 11 nm. The nanoparticle was dispersed well in aqueous solution and was stable without any aggregation. TCL-SPION and Feridex (control) solutions were prepared at 0.05, 0.1, 0.2 and 0.4 mg Fe/mL concentration. Their T2 relaxivity coefficients (r2) were measured with different echo time in a fast spin echo sequence (repetition time (TR)=4000 ms, echo time (TE)=10, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1400, 1500, 1800, and 1900 ms, field of view (FOV)=9, matrix=320×160, slice thickness=5 mm).
(3) Radiolabeling of Tyramine-Conjugated TCL-SPION with 124I
Radiolabeling of the tyramine-conjugated TCL-SPIONs with 124I was easily accomplished using commercially available Iodo-Beads (Pierce Biochemical Co., USA) according to the manufacturer's instructions. To put briefly, the beads were washed with PBS and dried on filter paper at room temperature. The washed beads were put in a [124I]NaI (1.85 mCi) solution in 100 μL of PBS. After shaking for 5 minutes, the [124I]NaI solution containing Iodo-Beads was mixed with an aqueous solution of the nanoparticle (152 μg, 20 μL of distilled water). Labeling was performed for 15 minutes at room temperature while gently shaking. The reaction was terminated by removing the beads from the reaction tube. The labeling yield was monitored on a silica plate using acetone as a developing solvent, using a radio-TLC imaging scanner (Bioscan Inc., Washington D.C., USA). The radiolabeling yield was 79%. Unlabeled free 124I ions were removed using a centrifugal filter (Microcon YM 50, Millipore Inc., USA). The radiochemical purity of was 92% after the purification.
(4) Comparison of Optical, microPET and MR Imaging
To evaluate the sensitivity of Cerenkov luminescence imaging, a series of solutions (300 μL) containing 124I and TCL-SPION were imaged by optical, microPET and MR imaging (
Referring to
b. The spatial resolution of Cerenkov imaging was studied using a Derenzo phantom having holes of different sizes filled with radioactive solutions. The core part of the Derenzo phantom was filled with a [124I]NaI solution (254 μCi in 8 mL distilled water). The luminescence and microPET images were obtained for 1 minute and 20 minutes, respectively. The PET images were reconstructed using the ordered subset expectation maximization 3D-MAP algorithm. The Derenzo phantom containing 32 μCi of 124I was imaged by luminescence and microPET imaging (
c. Animal experiments were conducted to investigate tissue penetration of Cerenkov imaging. A series of different concentrations of [124I]NaI in 10 μL volumes was injected into the back muscle (longissimus muscle group of the thoracic and lumbar vertebrae region) of Sprague-Dawley rats (ca. 420 g, Orient Bio Inc., Korea) at depths of 4 mm and 7 mm with increasing concentrations. The luminescence images were obtained using IVIS 200 after each injection for 1 minute. The rats were sacrificed by cervical dislocation just before radioactivity injection to prevent the fast diffusion of the injected radiation into the surrounding tissue, and the hair around the injection area was removed. All the luminescence images were obtained using IVIS 200 (Caliper Life Sciences, Inc., USA) in bioluminescence mode, with no excitation light source or emission filter.
(5) Triple-Modality Imaging of Radiolabeled Nanoparticles
Triple-modality images were obtained for a tumor metastasis model using the contrast agent for trimodality imaging prepared in Example 6, (3). Specifically, mouse breast cancer cell line 4T1 was cultured in RPMI 1640 containing 10% FBS and 1% antibiotics (penicillin and streptomycin) and maintained at 37° C. in a 5% CO2 incubator. The 4T1 tumor cells (1×107) were injected into the left shoulder of normal BALB/c mice (female, Orient Bio Inc., Korea). After 9 days, the tumor size grew to about 144 mm3. 124I-labeled nanoparticles (38 μg, 20 μCi) were subcutaneously injected into both front paws of the 4T1 tumor-bearing BALB/c mouse, and microPET imaging was performed for 30 minutes. MR imaging was performed successively at 1.5 T (GE Signa Excite 1.5T, USA) in a T2-weighted fast spin echo sequence. Luminescence imaging was performed for 1 minute. The dissected sentinel lymph node was microPET imaged for 20 minutes and then scanned for 3 minutes using IVIS 200.
As seen from
(6) Histological Examination and Immunohistochemistry
After the optical imaging in Example 6, (5), the mice were anesthetized using isoflurane/oxygen mixture gas. The lymph nodes and tumors were excised surgically, fixed using 10% neutral formalin, embedded in paraffin wax, and then stained with hematoxylin and eosin (H&E) and Prussian blue. For immunohistochemistry, intrinsic peroxidase activity was inhibited using 3% hydrogen peroxide. Then, antigens were retrieved with microwaves using 10 mmol/L citrate. Anti-cytokeratin 8 and 18 (CK8/18, Novocastra Laboratories, UK) were used for the immunostaining. The antigen-antibody complex was visualized by an avidin-biotin peroxidase complex solution using the ABC kit (Vector Laboratories, USA). Commercially available 3,3′-diaminobenzidine (Zymed, USA) was as a chromogen used for color development. The cells stained with Mayer's hematoxylin were counted.
Prussian blue staining is a method commonly used to detect Fe3+ in tissues. Prussian blue binds with Fe3+ in the tissue to produce bright blue color. Since TCL-SPION is an iron oxide (Fe3+)-based nanoparticle, the presence of TCL-SPION in the tissue can be detected as bright blue color through Prussian blue staining CK8/18 immunohistochemistry has been employed to detect epithelial-derived tumor cells. CK8/18 is expressed in high levels in the cytoplasm of tumor cells. A lot of tumor cells were metastasized to the left brachial lymph node and, hence, uptake of TCL-SPION was low. However, in the right brachial lymph node, only a few tumor cells were observed, and uptake of TCL-SPION was much higher in the right brachial lymph node than the left (
In conclusion, Cerenkov imaging showed great potential as a new optical imaging modality. Thanks to the intrinsic optical/PET dual-imaging properties of 124I, the extra step of fluorophore conjugation that is needed for optical imaging could be skipped, resulting in the simpler but higher yielding preparation of a hybrid nanoprobe for triple-modality imaging. The lymph node metastasis was screened with high sensitivity within a few minutes using an easily available optical imager, but the final diagnosis was based on accurate 3D visualization of accumulated nanoprobes with detailed anatomical information provided by PET and MR imaging. This facile method for the preparation of multimodality imaging probes based on Cerenkov imaging has a promising potential for application in many other biomedical and clinical research fields such as cell trafficking, tumor imaging and theragnosis.
Prior to luminescence imaging, mature arabidopsis (Arabidopsis thaliana, Columbia-O ecotype) had been kept in the dark for 60 minutes. The root of arabidopsis was immersed in a [32P]phosphoric acid (37 MBq/5 mL) solution and in 32P-free water (control). Immediately thereafter, a dynamic luminescence scan was performed with 2-minute exposure at every 5 minutes for 1 hour. The luminescence signal from the aqueous solution was blocked with black paper-covered aluminum foil. The luminescence intensity of selected leaves was quantified as a function of time.
The absorption pattern of phosphate by the plant was imaged in real time using [32P]phosphoric acid in luminescence mode (
Luminescence images of sequentially diluted [99mTc]TcO4− solutions (300 μL/well) were obtained for 5 minutes after blocking the wells containing radionuclides of different concentrations using black opaque paper. Only the 2.9 mCi/mL solution emitted very faint light (
[18F]FDG and [18F]KF having similar radioactivity in 300 μL of an aqueous solution were diluted sequentially by one third. The light intensity was measured using an in vitro luminometer. The relationship between the relative light unit (RLU) and radioactivity was plotted, and a straight line was drawn using the least-squares method for both radionuclides. There was no significant difference in luminescence between [18F]FDG and [18F]KF (
Thus, it was shown that, although [18F]FDG and [18F]KF are different in chemical forms with the former being a compound and the latter ion, the two do not exhibit significant difference in luminescence intensity.
The emission spectra were measured using FluoroLog-3 (Horiba Jobin Yvon Inc. USA). 3 mL of an aqueous solution of [124I]NaI (2.75 mCi) in a quartz cuvette was scanned before and after adding 10 μL, of quantum dots (Qdot® ITK™ 800, Invitrogen, USA), which emit maximum fluorescence at 800 nm. The scan time was 1 second per 1 nm wavelength with a total of 40 scans. Quantum dots of the same concentration were scanned in the same manner as the control. The luminescence intensity of [124I]NaI was much higher than the control solution of quantum dots without radioactivity (
Thus, it was shown that Cerenkov radiation may be employed as an internal light source and the signals and wavelengths may be controlled by attaching a fluorophore such as quantum dots. Also, tissue penetration may be improved therethrough.
A 131I-labeled compound spotted onto a C18 TLC plate was developed with a mobile phase (MeOH: 10% ammonium acetate buffer=3:7), and the plate was dried completely in air. The plate was scanned for 2 minutes using a radio-TLC imaging scanner (AR-2000, Bioscan, USA), and imaged for 1 minute using IVIS 200. The plate was covered with a transparent glass plate (1.2 mm thickness) prior to the optical imaging measurement in order to attain a higher luminescence signal. The relative intensities of the different spots were measured by counting the photons emitted from each selected spot. The percentage of the region of interest (ROI) from the luminescence imaging and the radio-TLC scanning was comparable (
Thus, it was shown that the luminescence intensity can be significantly improved by covering with a transparent glass plate having a high refractive index. Also, it was shown that a TLC plate can be imaged directly and quantified rapidly using optical imaging, and Cerenkov imaging might be a good adjuvant to radio-TLC scanning, particularly for radionuclides emitting little or no γ-rays.
First, lines were drawn on white paper with a pencil, and some lines were drawn over the lines with a 32P solution (1 mCi/100 μL in water). The luminescence images of the lines were obtained 1 minute before (left) and after (right) covering the paper with a 1.2 mm-thick transparent glass plate (
Those skilled in the art will appreciate that the conceptions and specific embodiments disclosed in the foregoing description may be readily utilized as a basis for modifying or designing other embodiments for carrying out the same purposes of the present disclosure. Those skilled in the art will also appreciate that such equivalent embodiments do not depart from the spirit and scope of the disclosure as set forth in the appended claims.
The contrast agent for optical imaging of the present disclosure is very useful in medical and bioscience industries since it allows easy obtainment of optical images.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2009-0100745 | Oct 2009 | KR | national |
This application is a divisional of U.S. application Ser. No. 13/503,256, filed Jul. 16, 2012, which is a national phase entry of International Application No. PCT/KR2010/007287, filed Oct. 22, 2010, which claims priority from Korean Patent Application No. 10-2009-0100745, filed Oct. 22, 2009, each of which is incorporated by reference herein in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 13503256 | Jul 2012 | US |
| Child | 14606504 | US |
