The present application is a National Phase entry of PCT Application No. PCT/EP2009/003893, filed May 30, 2009, which claims priority from German Application Number 102008026774.0, filed Jun. 4, 2008, 2008, the disclosures of which are hereby incorporated by reference herein in their entirety.
The invention relates to a controller for movable elements in microscope objectives for the adjustment of particularly hard-to-reach microscope objectives for ideal imaging quality and means for storing the adjustment movements of the movable elements.
It is known, as described in DE 198 04 470 C1, that for the adjustment to varying cover glass thicknesses, microscope objectives exhibit several lens group mounts, whereby one is designed as a correcting mount for the adjustment to varying cover glass thicknesses and axially movable between the lens group mounts, which are stationary with regard to the objective, whereby the correcting mount supports itself on the curve of a curve ring, arranged within the mount carrier, and which rotates. In order for the correcting mount to execute this shift, a lug, movable around the optical axis by means of a knurled ring in the mount carrier, is arranged at its end perpendicular to the optical axis. It is also known that the rotational movement of the movable elements is converted to a linear movement of the movable elements by means of a curved track. The disadvantage of using curves can be seen in the manufacture of the surfaces of said curves. Therefore, the evenness of the face of the produced curve in microscope objectives with regard to imaging quality of the microscope objective is very important. For achieving a sufficient surface quality, the faces of the curves must be ground in order to assure that no visible disturbances occur during the movement of the movable elements. Even though it is technically possible to grind a groove that is shaped as a curve, it is not applicable for reproducible serial production. A curve control via cam discs is utilized in the area of stereomicroscopy.
Here, the grinding of cam discs for increased surface quality is technically possible since it does not require the use of small grinders as for a groove in microscope objectives. Furthermore, the use of a curve control is advantageous due to the greater allowable tolerances in the area of stereomicroscopy. For the movement of the movable elements, a lever is also partially utilized for moving the movable elements. Even though a quick movement can be achieved via lever adjustment, a continuous movement of several movable elements together, as required in microscope objectives, cannot be achieved. Therefore, the adjustment via a lever is mainly suited for a quick positioning in the end positions.
In DE 101 59 239 A1, a microscope objective with motorized adjustable lenses for mapping of a sample is described. Said microscope objective is characterized in that all lenses together are arranged motorized adjustable within the lens housing, whereby said design serves for adjusting the scanning plane with a microscope objective. From DE 198 22 256 A1, a design for the direct control of the movement of a zoom system within a stereomicroscope is known, which consists of direct motorized drives for at least one movable lens group, whereby this design is a combination of motorized zoom and motorized focus. Furthermore, in DE 103 61 912 A1, a microscope objective with axially adjustable correcting mounts is described, whereby the axial movements of lens groups in a microscope objective for correcting of cover glass fluctuations and/or varying immersion media is provided via threaded rings. Thereby, corrections of the microscope objective are executed manually through the turning of a knurled ring. If the microscope objective is difficult to access, an adjustment for correcting the imaging quality on the microscope objective is very elaborate, i.e., if the observation position in a Petri dish changes, the bottom thickness of the Petri dish also changes. If said fluctuations are to be compensated in order to achieve a sharp high-contrast image, the knurled ring of the microscope objective must be turned. Therefore, for sample examinations with varying cover glass and bottom thicknesses, a microscope user must perform a variety of elaborate adjustments for positioning and attaining a very high imaging quality during the sample examination.
Based on said prior art, the invention is based on the task of creating a controller for microscope objectives for correcting the spherical aberration and for the adjustment of particularly hard-to-reach microscope objectives for best imaging quality, and which allows for an automatic adjustment of the varying movable elements of a microscope objective and, therefore, a simple, cost-efficient, user-friendly, and precise compensation, particularly, of cover glass fluctuations and varying bottom thicknesses of Petri dishes for improving the imaging quality.
For solving said task, the invention suggests a controller for movable elements in microscope objectives with which at least two movable elements of a microscope objective, designed as mounted lenses or lens groups, which are motor-driven in axial direction via respectively assigned adjusting rings along the optical axis of the microscope objective and are movable relative to the housing of the microscope objective. Thereby, in one embodiment a transmitting of the rotary movement of motors adjusting rings is advantageously effected by gear units, whereby a control unit, externally controlled and positioned within the microscope objective, for storing various characteristics for travel paths of the movable elements is provided.
An example embodiment is designed in such a way that the individual adjusting rings are separately movable by means of motors, assigned to the individual adjusting rings respectively, or that the adjustment of two or more movable elements along the optical axis of the microscope objective is coupled in such a way that, for example, two adjusting rings are partially or completely coupled and rotationally movable via a motor, or that all adjusting rings are coupled with each other and movable along the optical axis of the microscope objective, allowing therefore for simultaneous focusing.
A further advantage of the motorized control is the free configuration of the characteristics for travel paths for each motor, which, e.g., can be designed as positioning drive.
For example, each movable element can be positioned axially by an adjusting ring and a motor. The individual motors can be controlled in such a way that the movable element to be controlled travels along an axial, linear path and/or an axial curved path. During the axial movement of a movable element on a curved path, the individual path points are defined for the positioning drive and stored in the internal control unit. Therefore, with a thread adjustment, which effects a linear movement of the movable elements, via the stored characteristic for the positioning motor, it is possible to design the control of the motor in such a way that the rotational speed of the motor, controlled with the stored characteristic, results in an axial movement of the movable element on a curved path.
In an example embodiment, the characteristics for the travel paths of the movable elements of the microscope objective are stored in the internal control unit, whereby correcting of the imaging quality and focusing can be executed in accordance with the various characteristics for travel paths. As a result, every microscope objective can be switched on the stand, allowing for the use of a multiple turret or a switch of objectives during the measuring process. Furthermore, the storage of the characteristics for travel paths of the movable elements of every microscope objective in the internal control unit prevents collisions of the starting and end points of the travel paths of the individual movable elements, which vary due to mechanically caused tolerances.
In another example embodiment, the internal control unit receives its control commands from an external control unit which is positioned either in the microscope or a stationary control unit, allowing for an automated observation of samples while taking into account a continuously corrected imaging quality.
With the controller, according to the invention, the mechanical advantages of the thread adjustment can be used in such a way that in addition to the heretofore executed linear movements of the movable elements, said movable elements can also be moved along programmable curved paths, allowing for increased degrees of freedom for the layout of the optical design of a objective, which, among others, allow for a more cost-efficient construction of the design.
A further advantage of utilizing the controller is the option of focusing into the sample (internal focusing) while maintaining the image quality through appropriate adjustment of the movable elements. This is particularly significant for focusing with a microscope objective through a cover glass or the bottom of a Petri dish into an aqueous solution since the correcting and focusing position of the microscope objective must be adjusted due to the dispersion of light in water. As a result, the application of a microscope objective is further expanded. Until now it has only been possible to adjust the spherically corrected focal point of a microscope objective for varying cover glass thicknesses and/or immersion media directly at the cover glass or bottom of a Petri dish.
By means of the new controller for the movable elements it is also possible to now include in the calculations for the optical design additional values for the new positions of the movable elements for focusing in the sample, i.e., away from the cover glass or the bottom of a Petri dish. Additionally, said values are also stored in the internal control unit of the microscope objective. Therefore, with a microscope objective, the focus can be changed in such a way that a new focal point within the sample (internal focusing) can be mapped while maintaining imaging quality.
In the following, the invention is further explained by means of drawings with schematically shown embodiments.
In the embodiment in accordance with
During the axial movement of a movable element 2 on a curved path, the individual path points are defined for the positioning drive and stored in the internal control unit 15, so that with a thread adjustment, which effects a linear movement of the movable elements 2, via the stored characteristic for the motor 4, it is possible to design the control of the motor 4 in such a way that the rotational speed of the motor 4, controlled with the stored characteristic, results in an axial movement of the movable element 2 on a curved path. This results in variation of axial movement relative to rotational movement.
According to
Due to the rotational movement of the motors 4, the movable elements 2, designed to include lenses or lens groups, are moved along the optical axis of the microscope objective 1, whereby simultaneous focusing is possible. According to
The height adjustment of the movable element 2, effected by said movable element 2, designed as lenses or lens groups, in dependence of the rotation angle of the motor 4 is shown in diagrams.
Depending on the correction mode, the movable elements 2 are moved in accordance with varying characteristics, e.g., column 1=correction mode, column 2=focusing mode. As a result, every microscope objective 1 can be switched on the stand, allowing for the use of a multiple turret or a switch of microscope objectives 1. Since the characteristics and their references for the travel paths 16 of the movable elements 2 of the microscope objective 1 are stored in the internal control unit 15, the starting and end points, which vary due to mechanically caused tolerances, of the travel paths of the individual movable elements 2 can also be stored and no collisions occur. The internal control unit 15 receives its control commands from an external control unit 14, which can be positioned either in the microscope or in a stationary control unit.
The invention is not restricted to the embodiments but manifoldly variable within the scope of the disclosure.
1 Microscope objective
2 Movable element
3 Adjusting ring
4 Motor
5 Pinion
6 Sprocket
7 Internal thread
8 External thread
9 Inner bushing
10 Lug
11 Elongated hole
12 Fixed bushing
13 Bushing for lens holder
14 External control unit
15 Internal control unit
16 Characteristic for travel paths
Number | Date | Country | Kind |
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10 2008 026 774 | Jun 2008 | DE | national |
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/EP2009/003893 | 5/30/2009 | WO | 00 | 12/3/2010 |
Publishing Document | Publishing Date | Country | Kind |
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WO2009/146868 | 12/10/2009 | WO | A |
Number | Name | Date | Kind |
---|---|---|---|
4161756 | Thomas | Jul 1979 | A |
4329578 | Rosner | May 1982 | A |
4885600 | Iwasa | Dec 1989 | A |
5140458 | Takagi et al. | Aug 1992 | A |
5144491 | Ushiro et al. | Sep 1992 | A |
5825535 | Biber | Oct 1998 | A |
5856886 | Kaizu | Jan 1999 | A |
5930732 | Domanik et al. | Jul 1999 | A |
6023381 | Bender | Feb 2000 | A |
6366398 | Ouchi | Apr 2002 | B1 |
6504656 | Winterot et al. | Jan 2003 | B1 |
6909540 | Engelhardt et al. | Jun 2005 | B2 |
7230773 | Nomura | Jun 2007 | B2 |
7271966 | Herbst et al. | Sep 2007 | B2 |
7327514 | Uhl | Feb 2008 | B2 |
7599615 | Hsiao | Oct 2009 | B2 |
7679849 | Lule | Mar 2010 | B2 |
8094389 | Terada | Jan 2012 | B2 |
20020001126 | Engelhardt | Jan 2002 | A1 |
20030103263 | Engelhardt et al. | Jun 2003 | A1 |
20060182001 | Isobe et al. | Aug 2006 | A1 |
20070097495 | Knoblich | May 2007 | A1 |
20080025715 | Ishii | Jan 2008 | A1 |
20080266440 | Yazdanfar et al. | Oct 2008 | A1 |
20120188660 | Fahlbusch | Jul 2012 | A1 |
Number | Date | Country |
---|---|---|
26 11 639 | Sep 1977 | DE |
29 25 906 | Jan 1981 | DE |
690 23 387 | Apr 1996 | DE |
196 37 398 | Apr 1997 | DE |
198 04 470 | Aug 1999 | DE |
198 22 256 | Dec 1999 | DE |
101 59 239 | Jun 2003 | DE |
691 33 245 | Jan 2004 | DE |
103 61 912 | Jul 2005 | DE |
10 2004 034 992 | Feb 2006 | DE |
20 2008 006 065 | Aug 2008 | DE |
1 319 968 | Jun 2003 | EP |
1 532 576 | Nov 1978 | GB |
07-333480 | Dec 1995 | JP |
07-333480 | Dec 1995 | JP |
Entry |
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International Preliminary Report on Patentability for International Application No. PCT/EP2009/003893, dated Sep. 2, 2014, 7 pages. |
Number | Date | Country | |
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20110082590 A1 | Apr 2011 | US |