Claims
- 1. A synthetic nucleic acid encoding a protein selected from the group consisting of a γ-carboxylase comprising an amino acid sequence as set forth in SEQ ID NO:2 or 4 and a protein having at least 95% identity to said γ-carboxylase and capable of γ-carboxylating Conantokin G.
- 2. The synthetic nucleic acid of claim 1, wherein said nucleic acid is selected from the group consisting of a nucleic acid which comprises a nucleotide sequence as set forth in SEQ ID NO:1 or 3 and a nucleic acid which comprises a nucleotide sequence having at least 95% identity to the nucleotide sequence set forth in SEQ ID NO:1 or 3.
- 3. A vector comprising the nucleic acid of claim 1.
- 4. The vector of claim 3 which is an expression vector.
- 5. A vector comprising the nucleic acid of claim 2.
- 6. The vector of claim 5 which is an expression vector.
- 7. Host cells containing the vector of claim 3.
- 8. Host cells containing the vector of claim 4.
- 9. Host cells containing the vector of claim 5.
- 10. Host cells containing the vector of claim 6.
- 11. The host cells of claim 8 which further comprises an expression vector comprising a nucleic acid sequence encoding a conantokin.
- 12. The host cells of claim 10 which further comprises an expression vector comprising a nucleic acid sequence encoding a conantokin.
- 13. A method for producing γ-carboxylase which comprises growing the host cells of claim 8 under conditions suitable for growth and isolating the expressed γ-carboxylase.
- 14. A method for producing γ-carboxylase which comprises growing the host cells of claim 10 under conditions suitable for growth and isolating the expressed γ-carboxylase.
- 15. A method for producing γ-carboxylated conantokin which comprises growing the host cells of claim 11 under conditions suitable for growth and isolating the γ-carboxylated conantokin.
- 16. A method for producing γ-carboxylated conantokin which comprises growing the host cells of claim 12 under conditions suitable for growth and isolating the γ-carboxylated conantokin.
- 17. An isolated γ-carboxylase selected from the group consisting of a γ-carboxylase comprising an amino acid sequence as set forth in SEQ ID NO:2 or 4 and a protein having at least 95% identity to said γ-carboxylase and capable of γ-carboxylating Cononatokin G.
- 18. A method for producing γ-carboxylated conantokin which comprises combining together a γ-carboxylase and a conantokin propeptide containing a Conus γ-carboxylase recognition sequence to produce a γ-carboxylated conantokin and isolating the γ-carboxylated conantokin, wherein said γ-carboxylase is selected from the group consisting of a γ-carboxylase comprising an amino acid sequence as set forth in SEQ ID NO:2 or 4 and a protein having at least 95% identity to said γ-carboxylase and capable of γ-carboxylating Conantokin G.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] The present application is a continuation of U.S. patent application Ser. No. 10/213,439 filed 7 Aug. 2002. The present application is related to and claims priority under 35 U.S.C. §119(e) to U.S. provisional patent application Ser. No. 60/310,496 filed 8 Aug. 2001, incorporated herein by reference.
Government Interests
[0002] This invention was made with Government support under Grant No. PO1 GM48677 awarded by the National Institute of General Medical Sciences, National Institutes of Health, Bethesda, Md. The United States Government has certain rights in the invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60310496 |
Aug 2001 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
10213439 |
Aug 2002 |
US |
Child |
10788266 |
Mar 2004 |
US |