Core B: Qunatitative Analysis and Proteomics Core to Detect S-Nitrosylation

Information

  • Research Project
  • 8292292
  • ApplicationId
    8292292
  • Core Project Number
    P01ES016738
  • Full Project Number
    5P01ES016738-04
  • Serial Number
    16738
  • FOA Number
  • Sub Project Id
    9001
  • Project Start Date
    7/1/2011 - 12 years ago
  • Project End Date
    6/30/2013 - 10 years ago
  • Program Officer Name
  • Budget Start Date
    7/1/2011 - 12 years ago
  • Budget End Date
    6/30/2012 - 11 years ago
  • Fiscal Year
    2011
  • Support Year
    4
  • Suffix
  • Award Notice Date
    9/29/2011 - 12 years ago

Core B: Qunatitative Analysis and Proteomics Core to Detect S-Nitrosylation

The mission of this Quantitative Analysis and Proteomics Core (QAPC) will be to provide comprehensive proteomic approaches for the individual projects of this NIEHS Center to detect and characterize PD-related proteins, especially with regard to S-nitrosylation (reaction of the NO group with critical cysteine thiol to regulate protein function) and further oxidation reactions. Core B will support the efforts of Project 1, Project 2, and Project 3 by analysis and quantification of PDrelated proteins and their possible S-nitrosylation (including SNO-parkin, SNO-DJ-1, and SNOprotein disulfide isomerase [SNO-PDI]) using multiple chemical and proteomic approaches. Core B will also support the efforts of Project 4 by detecting possible biomarkers represented by these SNO-proteins in a-synuclein-over-expressing or mitochondrial complex I inhibitor-treated in hESCderived neuronal cell models. This Core will also interact with Core E (High-throughput Chemical Library Screening) by providing DAN assays that detect S-nitrosylated proteins in 384- and 1536- well multiwell formats in order to screen for small molecules that prevent S-nitrosylation of PDrelated proteins such as parkin, DJ-1 and PDI. These small molecules will then be tested for neuroprotection in PD model systems in Projects 2, 3, and 4.

IC Name
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
  • Activity
    P01
  • Administering IC
    ES
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
    123808
  • ARRA Funded
    False
  • CFDA Code
  • Ed Inst. Type
  • Funding ICs
    NIEHS:123808\
  • Funding Mechanism
    Research Projects
  • Study Section
    ZES1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    SANFORD-BURNHAM MEDICAL RESEARCH INSTIT
  • Organization Department
  • Organization DUNS
    020520466
  • Organization City
    LA JOLLA
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    920371005
  • Organization District
    UNITED STATES