Corylus plant named 'Wepster'

Information

  • Patent Application
  • 20150143594
  • Publication Number
    20150143594
  • Date Filed
    November 19, 2013
    11 years ago
  • Date Published
    May 21, 2015
    9 years ago
  • US Classifications
  • International Classifications
    • A01H5/00
Abstract
A new and distinct cultivar of Corylus plant named ‘Wepster’ characterized by high vigor and an upright-spreading plant habit, yellowish-green developing and fully expanded leaves during the spring and summer, resistance to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Muller, presence of random amplified polymorphic DNA markers 152-800 and 268-580 amplified by the polymerase chain reaction and which are linked to a dominant allele for resistance to eastern filbert blight from the cultivar Gasaway, expression of incompatibility alleles Si and S2 in the styles, and DNA fingerprints at 8 of 20 microsatellite marker loci differ from both parents OSU 440.005 and ‘Tonda Pacifica’, and from one parent at an additional 12 marker loci.
Description
BOTANICAL DENOMINATION


Corylus avellana


VARIETY DESIGNATION

‘Wepster’


BACKGROUND

The present Invention relates to a new and distinct cultivar of Corylus plant, botanically known as Corylus avellana, and hereinafter referred to by the name ‘Wepster’. Corylus avellana is in the family Betulaceae.


The new Corylus resulted from a controlled cross of female parent ‘Tonda Pacifica’ and male parent OSU 440.005 (unpatented) made in 1997 by Shawn A. Mehlenbacher and David C. Smith. ‘Tonda Pacifica’ is protected by U.S. Plant Pat. No. 22,715 (issued May 8, 2012). Hybrid seeds from the cross were harvested in August 1997, stratified, and seedlings grown in the greenhouse during the summer of 1998. From this cross, a total of 182 seedling trees were planted in the field in Corvallis, Oreg., USA in October, 1998. ‘Wepster’ was discovered and selected by the Inventors as a single plant within the progeny of the stated cross-pollination in a controlled environment in Corvallis, Oreg. It was originally assigned the designation OSU 894.030 (unpatented), which indicates the row and tree location of the original seedling. OSU 440.005 (unpatented) is from a cross of OSU 49.073×VR 8-32 (both unpatented). OSU 49.073 is from a cross of OSU 14.084בTombul Ghiaghli’ (both unpatented). OSU 14.084 is from a cross of ‘Barcelona’בDaviana’ (both unpatented). VR 8-32 is from a cross of ‘Montebello’ בGasaway’ (both unpatented). ‘Tonda Gentile delle Langhe’ (unpatented), the female parent of ‘Tonda Pacifica’, is an important cultivar in Piemonte, northern Italy. ‘Barcelona’, Oregon's most widely planted hazelnut cultivar, is known as ‘Castanyera’ (unpatented) where it originated in Catalunya, Spain. ‘Daviana’, originally from England, is the most widely planted pollinizer in ‘Barcelona’ orchards. ‘Tombul Ghiaghli’, obtained from Greece, is similar to the Turkish cultivar ‘Mincane’ (unpatented). ‘Montebello’ is widely grown under several names in Sicily. ‘Gasaway’ is the source of a single dominant gene for resistance to eastern filbert blight.


The new cultivar was asexually reproduced by rooted suckers annually for eight years (2004- 05 and 2007-12) in Corvallis, Oreg. The new cultivar was also asexually propagated by whip grafting in 2005 in Corvallis, Oreg. The unique features of this new Corylus are stable and reproduced true-to-type in successive generations of asexual reproduction.


SUMMARY

The following traits have been repeatedly observed and are determined to be the unique characteristics of ‘Wepster’. These characteristics in combination distinguish ‘Wepster’ as a new and distinct cultivar:


1. High vigor and upright-spreading plant habit.


2. Yellowish-green developing and fully expanded leaves during the spring and summer.


3. Resistance to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Muller.


4. Presence of random amplified polymorphic DNA markers 152-800 and 268-580 in DNA of ‘Wepster’ amplified by the polymerase chain reaction. These two markers are linked to a dominant allele for resistance to eastern filbert blight from the cultivar Gasaway (unpatented).


5. Expression of incompatibility alleles S1 and S2 in the styles.


6. DNA fingerprints at 8 of 20 microsatellite marker loci differ from both parents, ‘Tonda Pacifica’ and OSU 440.005, and from one parent at an additional 12 marker loci. Additional DNA fingerprints of grandparent ‘Tonda Gentile delle Langhe’, standard cultivar ‘Barcelona’, eastern filbert blight resistance source ‘Gasaway’ and five cultivars released by Oregon State University hazelnut breeding program are shown in attached Table 2.


In comparisons in two replicated trials conducted in Corvallis, Oreg., plants of the new Corylus differed from plants of the Corylus avellana cultivar ‘Barcelona’ (unpatented), and other cultivars and selections of Corylus avellana known to the Inventors primarily in nut size, nut shape, kernel percentage (ratio of kernel weight to nut weight), frequency of blank nuts (nuts lacking kernels), time of pollen shed, time of nut maturity, length of the husk or involucre, and plant size.





BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying colored photographs illustrate the overall appearance of the new cultivar, showing the colors as true as it is reasonably possible to obtain in colored reproductions of this type. Colors in the photographs may differ slightly from the color values cited in the detailed botanical description which accurately describe the colors of the new Corylus.



FIG. 1 shows typical nuts, raw kernels, and blanched kernels of ‘Wepster’ hazelnut (OSU 894.030) compared to those of ‘Yamhill’ hazelnut.



FIG. 2 shows the typical nuts, raw kernels, and blanched kernels of ‘Wepster’ hazelnut (OSU 894.030) compared to those of ‘Barcelona’, ‘Felix,’ and ‘York’ hazelnut cultivars.



FIG. 3 shows the blanched kernels of ‘Wepster’ hazelnut (OSU 894.030).



FIG. 4 shows the tree of the new cultivar ‘Wepster’ growing in a field in January, in Corvallis, Oreg.



FIG. 5 shows a pruned tree of the new cultivar ‘Wepster’ in January, in Corvallis, Oreg.



FIG. 6 shows the husks and nuts of ‘Wepster’ hazelnut.



FIG. 7 shows a tree of the new cultivar ‘Wepster,’ 8th leaf, growing in a field in the summer, in Corvallis, Oreg.





DETAILED DESCRIPTION

The cultivar ‘Wepster’ has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature and light intensity, without, however, any variance in genotype. The aforementioned images and following observations and measurements describe plants grown in Corvallis, Oreg. under commercial practice outdoors in the field during the fall, winter and spring. Plants used for the photographs and description were propagated by tie-off layerage and growing on their own roots, and about seven years old. In the following description, color references are made to The Royal Horticultural Society Colour Chart, 1966 Edition, except where general terms of ordinary dictionary significance are used.

  • Botanical classification: Corylus avellana cultivar Wepster.
  • Parentage:
      • Female, or seed, parent.—Corylus avellana cultivar Tonda Pacifica (U.S. Plant Pat. No. 22,715).
      • Male, or pollen, parent.—Corylus avellana selection OSU 440.005 (unpatented).
  • Propagation (type rooted suckers):
      • Time to initiate roots.—about 30 days at 20° C.
      • Time to produce a rooted young plant.—about six months at 22° C.
      • Root description.—Fine to thick; freely branching; creamy white in color.
  • Propagation (type whip grafting):
      • Time to budbreak on the scions.—about 14 days at 25° C.
      • Time to produce a grafted plant.—about six months at 25° C.
  • Plant description:
      • Type.—perennial shrub. Upright-spreading plant habit.
      • Growth and branching habit.—freely branching; about 15 lateral branches develop per plant. Pinching, i.e., removal of the terminal apices, enhances branching with lateral branches potentially forming at every node.
      • Size.—plant height. — About 6 meters; plant diameter or spread is about 6 meters.
      • Vigor.—high vigor growth habit.
  • Lateral branch description:
      • Length.—about 32 cm.
      • Diameter.—about 6 mm.
      • Internode length.—about 3.0 cm.
      • Texture.—smooth, glabrous.
      • Strength.—strong.
      • Color.—immature. — 152B; mature. — 152B.
  • Foliage description:
      • Arrangement.—alternate, simple.
      • Length.—about 10.2 cm.
      • Width.—about 9.1 cm.
      • Shape.—oblong to ovate.
      • Apex.—obtuse to acute.
      • Base.—cordate.
      • Margin.—serrate.
      • Texture, upper and lower surfaces.—slightly pubescent.
      • Venation pattern.—pinnate.
  • Color — Developing foliage, upper surface 144A, lower surfaces: 187A.
      • Fully expanded foliage, upper surface.—Spring and summer, 143A; late summer and fall, 143A.
      • Fully expanded foliage, lower surface.—Spring and summer, 139C; late summer and fall, 139C.
      • Venation, upper surface.—Spring and summer, 139C; late summer and fall, 139C.
      • Venation, lower surface.—Spring and summer, 139D; late summer and fall, 139D.
  • Petiole description:
      • Length.—about 2.7 cm.
      • Diameter.—about 1.8 mm.
      • Texture, upper and lower surfaces.—pubescent.
      • Color.—upper surface: Spring and summer, 139D; late summer and fall, 139D. lower surface: Spring and summer, 139D; late summer and fall, 139D.
  • Flower description:
      • Male inflorescences.—catkins, color prior to elongation 176C.
      • Female inflorescence.—style color 048B.
  • Nut description:
      • Length.—about 18.3 mm.
      • Width.—about 19.0 mm.
      • Depth.—about 16.6 mm.
      • Nut shape.—round.
      • Nut shape index [(width+depth)/2*length].—0.97.
      • Nut compression index (width/depth).—1.15.
      • Nut shell color.—164A.
      • Nut weight.—about 2.39 grams.
      • Kernel weight.—about 1.11 grams.
      • Kernel percentage (kernel weight/nut weight).—about 43.9%.
  • Disease/pest resistance: Plants of the new Corylus are highly resistant to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Muller. Plants of the new Corylus are highly resistant to bud mites (Phytoptus avellanae Nal.), while plants of ‘Tonda Gentile delle Langhe’ are highly susceptible, and plants of ‘Barcelona’ are highly resistant.
  • Temperature tolerance: Plants of the new Corylus have been observed to tolerate temperatures from -10 to 38 degrees C. in the field in Corvallis, Oreg.









TABLE 1







Primers and annealing temperatures for the 20 microsatellite marker


loci used to fingerprint ‘Wepster’ and other hazelnut cultivars.




















Repeat








Primers 5′-3′
Primers 5′-3′



motif
Locus
Size
Ta
n
He
Ho
PIC
r
LG
(forward)
(reverse)
Reference






















A614
(TC)17
125-156
60
14
0.85 
0.85
0.84
0.00
6
Hex-TGGCAGAG
R-GCAGTGGA
Gurcan et



(CA)10








CTTTGTCAGCTT
GGATTGCTGACT
al. 2010



NNN(CA)6








(SEQ ID NO: 1)
(SEQ ID NO: 2)






A616
(AC)11
136-162
60
13
0.85
0.85
0.83
0.00
8
Fam-CACTCATAC
R-ATGGCTTTT
Gurcan et












CGCAAACTCCA
GCTTCGTTTTG
al. 2010












(SEQ ID NO: 3)
(SEQ ID NO: 4)






A640
(CT)15
354-378
67
11
0.80
0.73
0.77
0.04
10
F-TGCCTCTGCAGTTA
Fam-CGCCATATAATT
Gurcan et



(CA)13








GTCATCAAATGTAGG
GGATGCTTGTTG
al. 2010












(SEQ ID NO: 5)
(SEQ ID NO: 6)






B617
(GA)15
280-298
60
9
0.80
0.78
0.78
0.01
8
Fam-TCCGTGTTGAGT
R-TGTTTTTGGTG
Gurcan et












ATGGACGA
GAGCGATG
al. 2010












(SEQ ID NO: 7)
(SEQ ID NO: 8)






B619
(TC)21
146-180
60
14
0.88
0.88
0.87
0.00
3
Fam-AGTCGGCTCC
R-GCGATCTGA
Gurcan et












CCTTTTCTC
CCTCATTTTTG
al. 2010












(SEQ ID NO: 9)
(SEQ ID NO: 10)






B634
(AG)15
218-238
60
9
0.76
0.76
0.73
0.00
4
Hex-CCTGCATCCA
R-GTGCAGAGG
Gurcan et












GGACTCATTA 60
TTGCACTCAAA
al. 2010












(SEQ ID NO: 11)
(SEQ ID NO: 12)






B671
(AG)6NN
221-249
60
13
0.86
0.88
0.84
−0.01
9
Hex-TTGCCAGTG
R-ACCAGCTCT
Gurcan et



(GA)17








CATACTCTGATG
GGGCTTAACAC
al. 2010












(SEQ ID NO: 13)
(SEQ ID NO: 14)






B709
(GA)21
219-233
60
16
0.87
0.80
0.86
0.04
8
Fam-CCACCAAC
R-GCGAAATGG
Gurcan et












TGTTTCACACCA
AGCTCTTGAAC
al. 2010












(SEQ ID NO: 15)
(SEQ ID NO: 16)






B733
(TC)15
161-183
60
8
0.68
0.68
0.63
0.00
7,2
Ned-CACCCTCTT
R-CATCCCCTG
Gurcan et












CACCACCTCAT
TTGGAGTTTTC
al. 2010












(SEQ ID NO: 17)
(SEQ ID NO: 18)






B749
(TC)12
200-210
60
6
0.60
0.64
0.51
−0.03
1
Hex-GGCTGACA
R-TCGGCTAGG
Gurcan et












ACACAGCAGAAA
GTTAGGGTTTT
al. 2010












(SEQ ID NO: 19)
(SEQ ID NO: 20)






B767
(TC)15
198-238
60
16
0.87
0.80
0.86
0.04
8
Fam-CCACCAACT
R-GCGAAATGG
Gurcan et



(AT)7








GTTTCACACCA
AGCTCTTGAAC
al. 2010












(SEQ ID NO: 21)
(SEQ ID NO: 22)






B774
(AG)15
195-213
60
8
0.80
0.80
0.77
0.01
5
Ned-GTTTTGCGA
R-TGTGTGTGGT
Gurcan et












GCTCATTGTCA
CTGTAGGCACT
al. 2010












(SEQ ID NO: 23)
(SEQ ID NO: 24)






B795
(TC)8NS
296-332
60
12
0.76
0.74
0.74
0.01
NA
Fam-GACCCACAA
R-TGGGCATCAT
Gurcan et



(CT)7NS








ACAATAACCTATCTC
CCAGGTCTA
al. 2010



(CT)10








(SEQ ID NO: 25)
(SEQ ID NO: 26)




NS(TC)5
















C115
(TAA)5
167-226
60
14
0.80
0.80
0.77
0.00
4
Fam-ATTTTCCGC
GTTTCCAGAT
Bassil et



(GAA)12








AGATAATACAGG
CTGCCTCCATATAAT
al. 2005b,












(SEQ ID NO: 27)
(SEQ ID NO: 28)
Gokirmak et














al. 2009





KG807
(TAAA)AA
226-248
54
4
0.67
0.78
0.60
-0.07
11
AAGCAAGAAAGGGA
FAM-CTTACAGATA
Gurcan and



(TAAA)2








TGGT
AATGGCTCAAA
Mehlenbacher



A(TAAA)2








(SEQ ID NO: 29)
(SEQ ID NO: 30)
2010





KG809
(AGG)6
333-345
55
5
0.66
0.64
0.60
0.01
4
Hex-AGGCATCA
F-GGAAGGTGA
Gurcan and












GTTCATCCAA
GAGAAATCAAGT
Mehlenbacher












(SEQ ID NO: 31)
(SEQ ID NO: 32)
2010





KG811
(GA)17
240-278
58
12
0.83
0.82
0.81
0.01
2
Ned-AAGGCGGCA
F-GAACAACTGAA
Gurcan and












CTCGCTCAC
GACAGCAAAG
Mehlenbacher












(SEQ ID NO: 33)
(SEQ ID NO: 34)
 2010





KG827
(CT)13AA
264-282
67
9
0.78
0.84
0.75
−0.04
9
Fam-AGAACTCCGAC
GAGGGAGCAAGTCAA
Gurcan and



(CA)7








TAATAATCCTAACCC
AGTTGAGAAGAAA
Mehlenbacher












TTGC
(SEQ ID NO: 36)
2010












(SEQ ID NO: 35)







KG830
(CT)14
279-311
67
9
0.79
0.78
0.76
0.00
9
Ned-TGGAGGAAGT
AAAGCAACTCATAGCT
Gurcan and



GTATT








TTTGAATGGTAGTAG
GAAGTCCAATCA
Mehlenbacher



(CA)8








AGGA
(SEQ ID NO: 38)
2010












(SEQ ID NO: 37)







Soman-G
(AAT)5
193-200
54
3
0.60
0.98
0.51
−0.27
NA
Hex-TGGCGTTGCA
R-GCCATCTTTAG
unpub-


(= 856 −









ACATATTCTC
AAAGTTCGATACAG
lished


MS1-13)









(SEQ ID NO: 39)
(SEQ ID NO: 40)






Primer fluorescent tags are FAM, HEX, and NED.


Ta: annealing temperature (° C.)


N: number of alleles


He: expected heterozygosity


Ho: observed heterozygosity


PIC: polymorphism information content


r: estimated null allele frequency


LG: linkage group


Reference: for development and characterization













TABLE 2







Allele sizes in ‘Wepster’ and other hazelnut cultivars at 20 microsatellite loci.




















Tonda
OSU
Tonda Gentile









Locus
Wepster
Pacifica
440.005
delle Langhe
Barcelona
Yamhill
Dorris
York
Felix
Santiam
Gasaway





A614
135/158
135/150
125/158
125/135
125/132
132/158
132/158
124/158
138/143
132/158
143/158


A616
152/160
150/160
152/160
150/152
144/152
150/150
150/152
144/152
150/152
150/152
150/150


A640
368/374
368/374
355/368
355/368
355/374
355/368
372/374
363/374
368/372
355/362
362/368


B617
293/295
293/293
293/295
285/295
285/289
289/295
287/295
287/289
287/287
285/295
291/295


B619
166/172
166/172
166/166
150/166
158/172
158/172
158/166
158/166
158/166
158/166
172/176


B634
228/228
228/228
228/234
228/228
228/228
236/236
228/228
228/236
228/236
222/236
222/234


B671
239/249
229/239
249/249
239/243
225/229
225/243
229/249
243/249
229/237
225/237
237/249


B709
229/235
229/235
223/229
229/229
227/235
229/229
229/229
229/233
229/233
229/229
229/229


B733
173/175
173/175
173/175
173/175
173/175
181/185
173/181
173/181
175/181
175/181
175/175


B749
207/209
207/209
209/209
207/209
209/209
209/209
207/207
209/209
207/207
209/209
207/209


B767
200/242
200/218
212/242
214/218
214/240
214/238
214/218
236/238
214/214
212/214
214/214


B774
203/207
203/207
203/211
203/211
203/207
203/211
203/207
203/209
203/213
209/213
203/209


B795
333/333
315/333
333/333
315/333
333/333
333/333
333/333
333/333
321/333
317/333
317/319


C115
183/194
174/183
194/216
174/174
174/194
197/216
194/216
197/197
197/216
194/197
216/219


KG807
252/252
228/252
252/252
238/252
238/252
230/252
242/252
242/252
238/242
242/252
242/252


KG809
342/342
339/342
342/348
339/342
339/339
348/348
339/348
339/348
339/348
339/342
339/348


KG811
257/257
245/257
257/267
257/267
261/267
251/261
257/267
257/257
251/267
257/267
257/261


KG827
270/282
270/284
272/282
268/278
282/284
268/282
272/284
268/272
272/284
272/272
272/282


KG830
295/305
291/295
295/305
291/295
291/295
291/295
295/297
295/295
293/303
291/295
291/305


Soman-G
196/200
196/200
196/196
196/200
196/200
196/200
196/200
196/200
196/200
196/200
196/196









REFERENCES

Bassil N. V., Botta R., Mehlenbacher S. A. 2005a. Microsatellite markers in hazelnut: Isolation, characterization and cross-species amplification. J. Amer. Soc. Hort. Sci. 130:543-549.


Bassil N. V., Botta R., Mehlenbacher S. A. 2005b. Additional microsatellite markers of the European hazelnut. Acta Hort. 686:105-110.


Boccacci P., Akkak A., Bassil N. V., Mehlenbacher S. A., Botta R. 2005. Characterization and evaluation of microsatellite loci in European hazelnut (C. avellana) and their transferability to other Corylus species. Molec. Ecol. Notes 5:934-937.


Boccacci P., Akkak, A. and Botta, R. 2006. DNA typing and genetic relations among European hazelnut (Corylus avellana L.) cultivars using microsatellite markers. Genome 49:598-611.


Gökirmak T., Mehlenbacher S. A., Bassil N. V. 2009. Characterization of European hazelnut (Corylus avellana) cultivars using SSR markers. Genetic Resources and Crop Evolution 56:147-172.


Gürcan, K., S. A. Mehlenbacher and V. Erdogan. 2010a. Genetic diversity in hazelnut cultivars from Black Sea countries assessed using SSR markers. Plant Breeding (available on-line doi :10. 1111/j. 1439-0523. 2009. 01753. x).


Gürcan, K., S. A. Mehlenbacher, N. V. Bassil, P. Boccacci, A. Akkak and R. Botta. 2010b. New microsatellite markers for Corylus avellana from enriched libraries. Tree Genetics and Genomes (available on-line as DOI 10.1007/s11295-010-0269-y).


Gürcan, K. and S. A. Mehlenbacher. 2010. Development of microsatellite marker loci for European hazelnut (Corylus avellana L.) from ISSR fragments. Molecular Breeding (available on-line).

Claims
  • 1. A new and distinct cultivar of Corylus plant named ‘Wepster’, as illustrated and described.
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT

This invention was made with government support under Specific Cooperative Agreement No. 58-5358-9-447 awarded by the United States Department of Agriculture. The government has certain rights in the invention.