Coumarin derivatives, and processes for their preparation

Abstract

The present invention relates to new coumarin derivatives represented by the general formula I ##STR1## wherein R.sup.1 =NH.sub.2 or --NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F,R.sup.1 =R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H,R.sup.1 =H or OH, R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H,R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2,R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3 or CF.sub.3, R.sup.4 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2,processes for the preparation thereof and their use as intermediates for the synthesis of new coumarin derivatives with potential biological action.

Description

The present invention relates to new coumarin derivatives represented by the general formula I ##STR2## wherein

R.sup.1 =NH.sub.2 or --NHCH.dbd.C(CO.sub.2 C.sub.2 H ).sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F, R.sup.1 =R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H, R.sup.1 =H or OH, R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 R=H, R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3 or CF.sub.3, R.sup.4 =--NHCH.dbd.C(C.sub.2 C.sub.2 H.sub.5).sub.2.

According to the present invention the new coumarin derivatives of the general formula I are prepared by reacting substituted coumarins of the formula II ##STR3## wherein

R.sup.1 =R.sup.3 =R.sup.4 =R.sup.5 =H, R.sup.2 =NH.sub.2, R.sup.1 =R.sup.3 =NH.sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H, R.sup.1 =OH, R.sup.2 =R.sup.4 =R.sup.5 =H, R.sup.3 =NH.sub.2, R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =NH.sub.2, R.sup.1 =N.sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F, R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3 or CF.sub.3, R.sup.4 =NH.sub.2, with diethyl-ethoxymethylene malonate of the formula III C.sub.2 H.sub.5 OCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2 III at the temperature of 120.degree. C. within a period from 10 minutes to 27 hours, whereat coumarin malonate esters of the general formula I with the above defined substituents are obtained.

The starting substituted aminocoumarins of the general formula II, with the exception of the compound with R.sup.1 =NH.sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F, have already been described in the literature: F. W. Linch, J. Chem. Soc. 101 (1912) 1758; G. T. Morgan, F. M. G. Micklethwait, J. Chem. Soc. 85 (1904)1230; G. Kokotos, C. Tzougraki, J. Heterocyclic Chem. 23 (1986) 87; I. C. Ivanov, S. K. Karagiosov, I. Manolov, Arch. Pharm. (Weinheim) 324 (1991) 61.

New coumarin derivatives of the general formula I are useful intermediates for the synthesis of new coumarin derivatives with a potential biological action such as antimicrobial, antitumour and antiviral action.

The present process is illustrated but in no way limited by the following examples.

EXAMPLE 1

3-amino-8-fluorocoumarin ##STR4##

A solution of 3-acetylamino-8-fluorocoumarin (3.00 g, 0.014 mole) in concentrated sulfuric acid (2.65 g, 0.027 mole) was heated at 75.degree.-80.degree. C. for 1 hour. The cooled solution was poured onto an ice-water mixture and it was alkalized with a saturated NaHCO.sub.3 water solution up to pH=6 and subsequently left to precipitate at +4.degree. C. The obtained 3-amino-8-fluorocoumarin was recrystallized from 50% ethanol (1.63 g, 67%). M.p.: 173.degree.-174.degree. C.

Analysis:

calculated for C.sub.9 H.sub.6 F.sub.3 NO.sub.2 : C=60.34; H=3.38; N=7.82. found: C=59.97; H=3.22; N=7.72.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 5.9 (s, NH.sub.2), 6.7 (s, H4); 7.1-7.2 (m, H5-H7). m/z: 178 (M.sup.-), 151, 141, 134, 127.

EXAMPLE 2

Diethyl-{�(2-oxo-2H�1!-benzopyrano-6-yl)amino!methylene}malonate ##STR5##

A solution of 6-aminocoumarin (2.00 g, 0.012 mole) in diethyl-etoxymethylene malonate (3.03 g, 0.014 mole) was heated for 10 minutes at 120.degree. C., whereat a solidification of the reaction mixture occurred. The obtained light brown precipitate of diethyl-{�(2-oxo-2H�1!-benzopyrano-6-yl)amino!methylene}malonate (3.95 g, 96%) was recrystallized from ethanol. M.p.: 141.degree.-142.degree. C.

Analysis:

calculated for C.sub.17 H.sub.17 NO.sub.6 : C=61.62; H=5.17; N=4.23. found: C=61.87; H=4.99; N=4.17.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 1.3 (t, CH.sub.3), 4.3 (q, CH.sub.2); 6.6 (d; H3); 7.7 (d, H8); 7.9 (d, H7), 8.5 (d; H5); 10.0 (d; H4); 12.4 (bs, NH).

EXAMPLE 3

Tetraethyl-{�(2-oxo-2H�1!-benzopyrano-3,6-di-yl)diamino!dimethylene}malonate ##STR6##

It was prepared according to the method described in Example 2 starting from 3,6-diaminocoumarin (1.00 g, 5.676 mmole) and diethyl-etoxymethylene malonate (2.81 g, 0.013 mole). Reaction duration: 6 hours. Recrystallization from ethanol gave yellow-green crystals of tetraethyl-{�(2-oxo-2H�1!-benzopyrano-3,6-di-yl)diamino!-dimethylene}malonate (2.55 g, 87%). M.p.: 131.degree.-134.degree. C.

Analysis:

calculated for C.sub.25 H.sub.28 N.sub.2 O.sub.10 : C=58.13; H=5.46; N=5.43. found: C=57.74; H=5.19; N=5.40.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 1.2 (t, CH.sub.3), 4.2 (q, CH.sub.2); 7.4 (m, C H), 7.6 (s, H5); 7.9 (s, H4); 8.2-8.5 (m; H7-H8); 10.6-10.8 (m; 2NH).

EXAMPLE 4

Diethyl-{�(3-hydroxy-2-oxo-2H�1!-benzopyrano-6-yl)amino!methylene}malonate ##STR7##

It was prepared according to the method described in Example 2 starting from 6-amino-3-hydroxycoumarin (0.88 g, 4.967 mmole). Reaction duration: 1 hour. The yellow-brown precipitate of diethyl-{�(3-hydroxy-2-oxo-2H�1!-benzopyrano-6-yl)-amino!methylene}malonate obtained by cooling the solution was recrystallized from glacial acetic acid (1.52 g, 88%).

Analysis:

calculated for C.sub.17 H.sub.17 NO.sub.7 : C=58.79; H=4.93; N=4.03. found: C=58.64; H=4.64; N=4.25.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 1.3 (t, CH.sub.3), 4.2 (q, CH.sub.2); 7.1-8.4 (m; Ar H); 10.5 (s; NH); 10.8 (d; OH). m/z: 347 (M.sup.+), 302, 149, 79, 61, 45, 43.

EXAMPLE 5

Diethyl-{�(3-hydroxy-2-oxo-2H�1!-benzopyrano-8-yl)amino!methylene}malonate ##STR8##

It was prepared according to the method described in Example 2 starting from 8-amino-3-hydroxycoumarin (1.93 g, 0.011 mole). Reaction duration: 4 hours. Recrystallization from glacial acetic acid gave a precipitate of diethyl-{�(3-hydroxy-2-oxo-2H�1!-benzopyrano-8-yl)amino!methylene}malonate in the form of brown shiny plate crystals (3.37 g, 89%).

Analysis:

calculated for C.sub.17 H.sub.17 NO.sub.7 : C=58.79; H=4.93; N=4.03. found: C=58.98; H=4.89; N=3.91.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 1.3 (t, CH.sub.3), 4.2 (q, CH.sub.2); 7.1-8.5 (m; Ar H); 10.7 (s; NH); 11.1 (d; OH). m/z: 347 (M.sup.+), 302, 273, 199, 177, 70, 61, 47.

EXAMPLE 6

Diethyl-{�(8-fluoro-2-oxo-2H�1!-benzopyrano-3-yl)amino!methylene}malonate ##STR9##

It was prepared according to the method described in Example 2 starting from 3-amino-8-fluorocoumarin (2.00 g, 9.250 mmole). Reaction duration: 5 hours. Cooling of the solution gave the precipitate of light yellow diethyl-{�(8-fluoro-2-oxo-2H�1!-benzo-pyrano-3-yl)amino!methylene}malonate, which was recrystallized from ethanol (2.76 g, 95%). M.p.: 175.degree.-177.degree. C.

Analysis:

calculated for C.sub.17 H.sub.16 FNO.sub.6 : C=58.45; H=4.62; N=4.01. found: C=58.38 H=4.46; N=3.98.

Example 7

Diethyl-{�(4-methyl-2-oxo-2H�1!-benzopyrano-7-yl)amino!methylene}malonate ##STR10##

It was prepared according to the method described in Example 2 starting from 7-amino-4-methylcoumarin (5.00 g, 0.029 mole). Reaction duration: 30 minutes. The precipitated diethyl-{�(4-methyl-2-oxo-2H�1!-benzopyrano-7-yl)amino!methylene}malonate was recrystallized from ethanol (9.70 g, 98%). M.p.: 139.degree.-140.degree. C.

Analysis:

calculated for C.sub.18 H.sub.19 NO.sub.6 : C=62.60; H=5.55; N=4.06. found: C=61.97 H=5.05; N=4.25.

EXAMPLE 8

Diethyl-{�(4(trifluoromethyl)-2-oxo-2H�1!-benzopyrano-7-yl)amino!methylene}malonate ##STR11##

It was prepared according to the method described in Example 2 starting from 7-amino-4-trifluoromethyl)coumarin (3.00 g, 0.015 mole). Reaction duration: 27 hours. By cooling yellow diethyl-{�(4-trifluoromethyl-2-oxo-2H�1!-benzopyrano-7-yl)-amino!methylene}malonate was precipitated, which was recrystallized from ethanol (4.83 g, 92%). M.p.: 125.degree.-126.degree. C.

Analysis:

calculated for C.sub.18 H.sub.16 F.sub.3 NO.sub.6 : C=54.14; H=4.04; N=3.51. found: C=54.12 H=4.17; N=3.46.

.sup.1 H-NMR (DMSO-d.sub.6). .delta./ppm: 1.3 (t; CH.sub.3); 4.2 (q; CH.sub.2); 6.8 (s, H3), 7.4-7.6 (m; H6, H8, C H); 8.4 (d, H5); 10.7 (d; NH).

Claims

1. Coumarin derivative of the formula I ##STR12## wherein R.sup.1 =NH.sub.2 or --NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F,

R.sup.1 =R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H,

R.sup.1 =OH, R.sup.3 =NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H,

R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, or

R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3 or CF.sub.3, R.sup.4 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2.

2. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =NH.sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F.

3. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.2 =R.sup.4 =R.sup.5 =H, R.sup.1 =R.sup.3 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2.

4. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =OH, R.sup.3 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.4 =R.sup.5 =H.

5. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2.

6. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =--NHCH .dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F.

7. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3, R.sup.4 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2.

8. Coumarin derivative of the formula I of claim 1, characterized in that R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CF.sub.3,R.sup.4 =--NHCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2.

9. A process for the preparation of a compound of the formula I of claim 1, characterized in that a compound of the formula II ##STR13## wherein R.sup.1 =R.sup.3 =R.sup.4 =R.sup.5 =H, R.sup.2 =NH.sub.2,

R.sup.1 =R.sup.3 =NH.sub.2, R.sup.2 =R.sup.4 =R.sup.5 ==H,

R.sup.1 =OH, R.sup.2 =R.sup.4 =R.sup.5 =H, R.sup.3 =NH.sub.2,

R.sup.1 =OH, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =NH.sub.2,

R.sup.1 =NH.sub.2, R.sup.2 =R.sup.3 =R.sup.4 =H, R.sup.5 =F, or

R.sup.1 =R.sup.3 =R.sup.5 =H, R.sup.2 =CH.sub.3 or CF.sub.3, R.sup.4 =NH.sub.2,

and diethyl-ethoxymethylene malonate of the formula III

C.sub.2 H.sub.5 OCH.dbd.C(CO.sub.2 C.sub.2 H.sub.5).sub.2 III

are heated at the temperature of 120.degree. C. within a period from 10 minutes to 27 hours, followed by cooling, whereat coumarin malonate esters are precipitated.