Claims
- 1. A particle reagent having high refractive index consisting essentially of:
- (A) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (1) an ethylenically unsaturated monomer having a functional group capable of reacting with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (2) optionally, other ethylencially unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (3) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerization in the presence of said inner core; and
- wherein said polymer particle has an approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (B) a compound of biological interest or its antibody.
- 2. The particle reagent of claim 1 wherein said outer shell is not more than 5 parts by weight of said polymer particle.
- 3. A particle reagent having high refractive index consisting essentially of:
- (A) a polymer particle having an inner core and and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (1) an ethylenically unsaturated monomer having a functional group capable of reacting with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (2) optionally, other ethylenically unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (3) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerizable in the presence of said inner core; and
- wherein said polymer particle has an approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (B) a compound of biological interest through a proteinaceous material.
- 4. A method for measuring compounds of biological interest comprising the steps of
- (A) incubating
- (1) a particle reagent having high refractive index essentially of:
- (a) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (i) an ethylenically unsaturated monomer having a functional group capable of reacting with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (ii) optionally, other ethylenically unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (iii) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerization in the presence of said inner core and wherein said polymer particles has approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (b) the compound of biological interest or its antibody;
- (2) a liquid suspected of containing the compound of biological interest; and
- (3) an agglutinating agent; and
- (B) photometrically measuring increased particle size resulting from agglutination.
- 5. The method of claim 4 wherein the polymer particle is covalently attached to the compound of biological interest.
- 6. The method of claim 5 wherein the agglutinating agent is selected from the group consisting of
- (A) an antibody to the compound of biological interest; and
- (B) a particle reagent having high refractive index consisting essentially of:
- (1) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (a) an ethylenically unsaturated monomer having a functional group capable of reaching with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (b) optionally, other ethylenically unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (c) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerization in the presence of said inner core and wherein said polymer particles has an approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (2) an antibody to the compound of biological interest.
- 7. The method of claim 4 wherein the polymer particle is covalently attached to an antibody of the compound of biological interest.
- 8. The method of claim 7 wherein the agglutinating agent is a multivalent conjugate of the compound of biological interest and a protein.
- 9. The method of claim 4 wherein during the incubation step (a) there is also present an agglutinating accelerator.
- 10. The method of claim 9 wherein the agglutinating accelerator is selected from the group consisting of polyethyene glycol and sodium dodecyl sulfate.
- 11. The method of claim 5 wherein the polymer particle is covalently attached to the compound of biological interest through a proteinaceous material.
- 12. A particle reagent having high refractive index consisting essentially of:
- (A) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (1) an ethylenically unsaturated monomer having a functional group capable of reacting with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (2) optionally, other ethylenically unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (3) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerization in the presence of said inner core; and
- wherein said polymer particle has an approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (B) an antigen of the compound of biological interest.
- 13. The particle reagent of claim 12 wherein said outer shell is not more than 5 parts by weight of said polymer particle.
- 14. The particle reagent of claim 12 wherein the antigen of the compound of biological interest is attached to said polymer through a proteinaceous material.
- 15. A method for measuring proteins comprising the steps of
- (A) incubating
- (1) a particle reagent having high refractive index consisting essentially of:
- (a) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of
- (i) an ethylenically unsaturated monomer having a functional group capable of reacting with a compound of biological interest selected from the group consisting of epoxy, carboxyl, amino, hydroxyl and aldehyde,
- (ii) optionally, other ethylenically unsaturated monomers in an amount sufficient to produce water insoluble polymer particles, and
- (iii) not more than 10 parts by weight of the outer shell of the monomers of the inner core, said outer shell being formed by polymerization in the presence of said inner core and wherein said polymer particles has an approximate diameter range of 0.03-0.1 .mu.m and is covalently attached to
- (b) the antibody of the protein; and
- (2) a liquid suspected of containing the compound of biological interest; and
- (B) photometrically measuring increased particle size resulting from agglutination.
CROSS REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of application Ser. No. 298,473, filed Sept. 1, 1981, now abandoned.
US Referenced Citations (7)
Non-Patent Literature Citations (1)
Entry |
H. N. Eisen, "Immunology", Harper and Row, 1974, pp. 391-394. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
298473 |
Sep 1981 |
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