Patent Application
20230414844
The present application claims priority from Chinese Patent Application No. 202210729218.5 filed on Jun. 24, 2022, the contents of which are incorporated herein by reference in their entirety.
The present disclosure belongs to the technical field of medical cosmetology and relates to a soluble hydrogel microneedle and a method for preparing the same.
In the cosmetic and medical industry, for operations involving subcutaneous implantation, such as minimally invasive surgery, drug delivery and subcutaneous sampling, a common method is needle-based injection or extraction. Needle-based injection will produce sensory pain and cause greater psychological pressure to the patient. In addition, improper use of needle syringes may cause infection or allergies, causing certain safety hazards. Transdermal Drug Delivery System or Trandermal Thrapeutic System (TTS for short) is a method for absorbing drug through the skin. After the drug is absorbed through the skin, it enters the blood circulation of the human body, reaches an effective blood drug concentration, and realizes disease treatment or prevention. Compared with traditional oral drug delivery, it avoids liver first-pass effect, gastrointestinal inactivation and the like; and compared with injection drug delivery, it avoids the pain caused by subcutaneous injection and improves patient compliance. However, the tight stratum corneum in human skin not only plays a protective role, but also hinders the absorption of drugs. As an effective and minimally invasive novel mode of transdermal drug delivery, microneedle technology has attracted more and more attention for its application potential. Microneedles have the advantages such as painless, minimally invasive and efficient drug delivery, which can enhance transdermal drug delivery by piercing human skin, destroying skin barrier (stratum corneum) and improving skin permeability. This technology has been shown to increase transdermal delivery of a variety of biomolecules, including small molecule drugs, vaccines, proteins, DNA and the like.
Hydrogels, three-dimensional polymeric materials with chemical or physical cross-linked structures, have been used as novel microneedle materials. Among them, polyvinyl alcohol, as a polymer material with good biocompatible mechanical strength, can be cross-linked into hydrogel through freeze-thaw, irradiation and other means without adding a cross-linking agent, and thus can be applied to the field of hydrogel microneedle. The hydrogel microneedles formed by polyvinyl alcohol are in a rigid glassy state when it is dried, and can be easily pierced into the skin. After piercing into the skin and absorbing the liquid, they change from the glassy state to a soft gel state, therefore avoiding damage to the subcutaneous cells, eliminating the hidden danger of breaking the rigid microneedles under the skin, causing no significant pain, causing minimal damage to the tissue during use, and posing minimal threat to the environment after treatment.
In recent years, in-depth study on preparation of polyvinyl alcohol microneedle products by the freeze-thaw method has been conducted in China. Among them, Chinese patent with publication number of CN109701152A introduces a soluble microneedle patch prepared from polyvinyl alcohol-dextran solutions with different mass fractions via centrifugation, freezing and thawing, which has good mechanical properties and can pierce the skin; and Chinese patent with publication number of CN110090044A introduces a hydrogel microneedle patch prepared by mixing polyvinyl alcohol and a highly hydrophilic polymer solution, repeatedly freeze-thawing the resulting mixed solution to form cross-linked ice crystals of polyvinyl alcohol, drying and then demoulding. During use, the hydrogel microneedle patch with extracted skin interstitial fluid is immersed in a buffer solution and warmed to a suitable temperature to dissolve the cross-linked ice crystals of the hydrogel microneedle patch in the buffer solution, so as to release the target substance.
However, the cross-linked network structure formed by physical cross-linking through the freeze-thaw method alone is unstable and varies with temperature, and the hydrogel will be thawed when the temperature rises to a certain level. Therefore, the microneedle products prepared by the freeze-thaw method cannot meet the requirements of long-term transportation and storage. When an irradiation method is used for cross-linking, the stability of the prepared hydrogel is improved, but the flowing uncross-linked solution needs to be fixed in a mold for irradiation, and air bubbles are easily brought in during the transport process, which affects the state and properties of the product. In addition, the molds take up a lot of volume, which reduces the yield and increases the difficulty of mass production.
The purpose of the present disclosure is to provide a cross-linked hydrogel microneedle for transdermal drug delivery and a method for preparing the same, so as to overcome the problem of poor stability for the preparation by a freeze-thaw method and the problem of easy introduction of air bubbles for the preparation by an irradiation method.
According to a first aspect of the present disclosure, the following technical solutions are used in the present disclosure.
A method for preparing cross-linked hydrogel microneedles for transdermal drug delivery, comprising subjecting a mixed solution of sodium hyaluronate and polyvinyl alcohol to a freeze-thaw cross-linking step to eliminate the fluidity of the mixed solution; demoulding a product obtained from the freeze-thaw cross-linking step to obtain preformed microneedles; and subjecting the preformed microneedles to an irradiation cross-linking to obtain the cross-linked hydrogel microneedles.
Further, in the mixed solution, a mass concentration of polyvinyl alcohol is 1% to 30% and a mass concentration of sodium hyaluronate is 0.1% to 5%.
Further, the preparation method includes the following steps:
(1) preparing the mixed solution: adding polyvinyl alcohol into water and dissolving the polyvinyl alcohol solution in a water bath at 50° C. to 95° C. with stirring; adding sodium hyaluronate into water and dissolving to form the sodium hyaluronate solution with stirring; and mixing the sodium hyaluronate solution with the polyvinyl alcohol solution cooled to room temperature with stirring and defoaming under vacuum;
(2) freeze-thaw cross-linking: pouring the mixed solution obtained from step (1) into a mold, and performing freeze-thaw at 0° C. or below several times until obtaining a gel with no fluidity;
(3) demoulding the gel to obtain the preformed microneedles; and
(4) irradiation cross-linking: further cross-linking the preformed microneedles obtained from step (3) by irradiation to obtain stable hydrogel microneedles, and then performing dehydrating and drying to obtain a final product.
Further, a mode of the irradiation cross-linking is electron beam or gamma ray at an irradiation dose of 10 to 50 kGy.
Further, a drying method is one of blast air oven drying, oven drying, vacuum drying, and freeze drying.
According to a second aspect of the present disclosure, the following technical solutions are used in the present disclosure.
A cross-linked hydrogel microneedle for transdermal drug delivery is provided, which is prepared by the above preparation method.
According to the two-step cross-linking method of freeze-thaw and irradiation used by the present disclosure, the uncross-linked solution with fluidity is firstly freeze-thawed to obtain a preformed microneedle without fluidity, so that it can be released from a mold and the introduction of air bubbles during the transport process is avoided, and then the preformed hydrogel-like microneedle is further subjected to irradiation cross-linking to increase the stability of the microneedle, and the hydrogel microneedle is sterilized at the same time.
The present disclosure has the following beneficial effects:
The present disclosure is further described in detail below with reference to embodiments. The following embodiments are merely intended to describe the present disclosure rather than to limit the scope of the present disclosure. Non-essential improvements and adjustments made by a person skilled in the art under the core guiding ideology of the present disclosure still fall within the protection scope of the present disclosure.
The numbers of air bubbles for the microneedles described in Example 1, Example 2, and Comparative Example 1 were compared. The above microneedles were cut into three 5 cm×5 cm squares, which were placed in a clarity tester to visually check the number of air bubbles under 1000 l×illumination. The results are shown in Table 1.
The above results show that the microneedles prepared by irradiation after freeze-thaw preforming are able to reduce the generation of air bubbles significantly compared to the microneedles prepared by direct irradiation.
The general description and specific examples used above are only used to help illustrate the present disclosure, and are intended to better explain the principles and practical applications of the present disclosure, rather than being used to limit the present disclosure. Modifications or improvements made by a person skilled in the art without departing from the spirit or essential features of the present disclosure should all fall within the scope of the present disclosure.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202210729218.5 | Jun 2022 | CN | national |
