Patent Application
20220088570
The described and claimed inventive concept(s) relate to the use of cross-linked polymeric ammonium salts for absorbing and desorbing organic contaminants, and, more particularly, to the use of such cross-linked polymeric ammonium salts for absorbing and desorbing at least one or more Per and Polyfluoro Alkyl Substances (PFAS) from water, by changing the pH.
Per- and Polyfluoroalkyl substances (PFAS) have been shown to be highly persistent in the environment and in biological tissue and have been correlated with negative health impacts. According to the Agency for Toxic Substances and Disease Registry (ATSDR), PFAS increase cholesterol and suppress the immune system. PFAS can bio-accumulate, some having very long haft-lives in humans, and they are found in the blood of a large percentage of the U.S. population. They are very stable chemicals that can persist in soil and water for long periods of time, and they are highly mobile in soils and water.
PFAS encompass a whole family of manmade chemicals used in consumer and industrial applications, such as, for example, in the fabrication of non-stick cookware, grease-resistant paper, fast food wrappers, microwave popcorn bags, stain-resistant carpets and fabrics, water-resistant clothing and in cleaning and personal care product formulations and in aqueous film-forming foams (AFFF) for fire suppression. There are more than 3,000 PFAS chemicals that are in current use, or have previously been used, on the global market. While the origin of the environmental contamination is not known in most cases, current focus seems to be on Aqueous Film-Forming Foams (AFFF's) as 75% of the contaminated sites reported to date have some association with AFFF's. PFAS surfactant-containing AFFF formulations have been used extensively to extinguish hydrocarbon fuel fires at military bases, fire training sites, and oil refineries.
The available conventional water treatment systems and methods to remove PEAS from water have shortcomings. Granular activated carbon (GAC) adsorption systems and methods used to remove PEAS from water, for example, have been shown to be somewhat effective on the longer-chain PEAS, but are less effective in removing branched and shorter chain compounds. Similar to activated carbon, some conventional anion exchange resins (IX) may be more effective at removing longer chain PEAS than the shorter chain compounds. Other anion exchange resins have shown some success in removing a broader range of PFAS, including the shorter-chain compounds. However, removal of the PEAS to recover the ion exchange resins for re-use can be difficult. In addition, these sorbents have some deficiencies when used to remediate well and river waters. For example, PFAS concentrations in these waters are usually orders-of-magnitude lower than background constituents (ppt being low vs. ppb being high), such as natural organic matter (NOM) and metal ions, which compete with PFAS for sorption sites with the result that PEAS removal is reduced.
Though materials containing amine functional groups have been shown to absorb PEAS, in these types of materials, amine functionality and porosity of the sorbents play a key role on PEAS removal efficiency, kinetics, and capacity. The strategy of incorporating swell and de-swell properties has never been reported with amine functionalized PEAS sorbents.
There is a critical need, therefore, to develop PFAS sorbents that exhibit rapid PEAS removal of all chain lengths and facile regeneration through desorption wherein three design elements are incorporated: (i) provision of a molecular environment that balances lipophilic and hydrophilic forces to attract amphiphilic PEAS molecules; (ii) exhibition of an ability to tune the chain length of lipophilic blocks to match the chain length of the PEAS molecules; and (iii) exhibition of an ability to vary cross-link density thereby affecting swell levels.
The inventive concept(s) described and claimed herein relate to a method for absorbing, i.e., removing, at least one or more PFAS molecules from an aqueous medium wherein the PFAS molecules comprise fluorinated amphiphilic structures with carbon chain lengths ranging from 4 to 14 carbon atoms. The PFAS molecules are contacted with at least one crosslinked polymeric ammonium salt, or a mixture of said crosslinked polymeric ammonium salts, wherein in the salts about 25% or more of the groups which link ammonium nitrogen atoms are group Y, wherein Y is an n-alkylene group or alkyl substituted n-alkylene group, wherein the n-alkylene group or the alkyl substituted n-alkylene group has from 2 to about 20 carbon atoms. From zero to about 75% of the groups which link ammonium nitrogen atoms are group Z, wherein Z is a hydrocarbylene radical containing from 2 to 50 carbon atoms, and the hydrocarbylene radicals optionally contain or are substituted with one or more hydroxyl, ether, amino. thioether, keto, ester, silyl group or heterocyclic rings. About 25% or more of the ammonium nitrogen atoms are secondary ammonium nitrogen atoms.
According to one embodiment, best results are believed to occur when the hydrocarbylene radicals contain from 1 to 30 carbon atoms.
According to an alternate embodiment, the crosslinked polymeric ammonium salts contemplated for use herein have a swell factor of at least about 2 in water.
According to another embodiment, the crosslinked polymeric ammonium salt is a poly(alkylamine) ammonium salt.
According to another embodiment, the poly(alkylamine) ammonium salt is prepared from hexamethylene diamine and 1,10-dibromodecane using DMF/methanol as solvent.
According to another embodiment, the poly(alkylamine) ammonium salt is prepared from polyethylene imine and dibromodecane using DMF/methanol as solvent.
According to yet another embodiment, the described and claimed inventive concept(s) includes the additional steps of (i) desorbing the PFAS molecules from the at least one crosslinked polymeric ammonium salt, or from the mixture of crosslinked polymeric ammonium salts, by contacting the at least one crosslinked polymeric ammonium salt which contains PFAS molecules with an aqueous alkaline solution having a pH in the range of from about 8 to 14 with the result that the PFAS molecules are released from the at least one crosslinked polymeric ammonium salt, or from the mixture of crosslinked polymeric ammonium salts, and (ii) recovering the PFAS molecules and the at least one crosslinked polymeric ammonium salt or the mixture of crosslinked polymeric ammonium salts.
According to another embodiment, the alkaline solution is prepared from ammonium hydroxide and methanol.
According to another embodiment, the aqueous alkaline solution is prepared from sodium hydroxide and water.
According to another embodiment, the PFAS molecules comprise telomer alcohols of the type used in aqueous fire-fighting foam compositions.
According to another embodiment, the described and claimed crosslinked polymeric ammonium salts can be deployed in polar organic chemical integrative samplers (POCIS).
The aqueous media contemplated for application of the described and claimed inventive concept(s) comprise at least one of stagnant pools, wells, rivers, springs, estuarine systems, and industrial and municipal wastewater streams.
b
1, 24b2, 24b3 and 24b4 show results from the desorption step in Example 18 for HG-1 and HG-5, using ammonium hydroxide and methanol; ammonium hydroxide and water; sodium hydroxide and water; sodium carbonate; and ammonium carbonate.
Before explaining at least one embodiment of the presently disclosed and claimed inventive concept(s) in detail, it is to be understood that the presently disclosed and claimed inventive concept(s) is not limited in its application to the details of construction and the arrangement of the components, steps or methodologies set forth in the following description or illustrated in the drawings. The presently disclosed and claimed inventive concept(s) is capable of other embodiments or of being practiced or carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein is for the purpose of description and should not be regarded as limiting.
Unless otherwise defined herein, technical terms used in connection with the presently disclosed and claimed inventive concept(s) shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.
All patents, published patent applications, and non-patent publications mentioned in the specification are indicative of the level of skill of those skilled in the art to which this presently disclosed and claimed inventive concept(s) pertains. All patents, published patent applications, and non-patent publications referenced in any portion of this application are herein expressly incorporated by reference in their entirety to the same extent as if each individual patent or publication was specifically and individually indicated to be incorporated by reference.
All of the articles and/or methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the articles and methods of the presently disclosed and claimed inventive concept(s) have been described in terms of particular embodiments, it will be apparent to those of skill in the art that variations may be applied to the articles and/or methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the presently disclosed and claimed inventive concept(s). All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the presently disclosed and claimed inventive concept(s) as defined by the appended claims.
As utilized in accordance with the present disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings:
Use of the word “a” or “an” when used in conjunction with the term “comprising” in the claims and/or the specification may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.” Use of the term “or” in the claims is used to mean “and/or” unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive, although the disclosure supports a definition that refers to only alternatives and “and/or.” Throughout this application, the term “about” is used to indicate that a value includes the inherent variation of error for the device, the method being employed to determine the value, or the variation that exists among the study subjects. For example, but not by way of limitation, when the term “about” is utilized, the designated value may vary by plus or minus twelve percent, or eleven percent, or ten percent, or nine percent, or eight percent, or seven percent, or six percent, or five percent, or four percent, or three percent, or two percent, or one percent. The use of the term “at least one” will be understood to include one as well as any quantity more than one, including but not limited to, 2, 3, 4, 5, 10, 15, 20, 30, 40, 50, 100, etc. The term “at least one” may extend up to 100 or 1000 or more, depending on the term to which it is attached; in addition, the quantities of 100/1000 are not to be considered limiting, as higher limits may also produce satisfactory results. In addition, the use of the term “at least one of X, Y and Z” will be understood to include X alone, Y alone, and Z alone, as well as any combination of X, Y and Z. The use of ordinal number terminology (i.e., “first,” “second,” “third,” “fourth,” etc.) is solely for the purpose of differentiating between two or more items and is not meant to imply any sequence or order or importance to one item over another or any order of addition, for example.
As used in this specification and claim(s), the words “comprising” (and any form of comprising, such as “comprise” and “comprises”), “having” (and any form of having, such as “have” and “has”), “including” (and any form of including, such as “includes” and “include”) or “containing” (and any form of containing, such as “contains” and “contain”) are inclusive or open-ended and do not exclude additional, unrecited elements or method steps.
The term “or combinations thereof” as used herein refers to all permutations and combinations of the listed items preceding the term. For example, “A, B, C, or combinations thereof” is intended to include at least one of: A, B, C, AB, AC, BC, or ABC, and if order is important in a particular context, also BA, CA, CB, CBA, BCA, ACB, BAC, or CAB. Continuing with this example, expressly included are combinations that contain repeats of one or more item or term, such as BB, AAA, AAB, BBC, AAABCCCC, CBBAAA, CABABB, and so forth. The skilled artisan will understand that typically there is no limit on the number of items or terms in any combination, unless otherwise apparent from the context.
As used herein, the term “substantially” means that the subsequently described event or circumstance completely occurs or that the subsequently described event or circumstance occurs to a great extent or degree. For example, the term “substantially” means that the subsequently described event or circumstance occurs at least 90% of the time, or at least 95% of the time, or at least 98% of the time.
The sorbent materials according to the inventive concept(s) described herein are crosslinked polymeric ammonium salts. Such sorbent materials may also be referred to as poly(alkylamine) derived ionomer hydrogels as discussed in more detail below. The term “crosslinked” is used herein to mean a polymer which has a network structure. A common test to determine if a polymer is crosslinked is to attempt to dissolve the polymer in a liquid that is normally a solvent for that polymer. Linear or branched, but not crosslinked, polymers will dissolve in the solvent. Crosslinked polymers typically do not dissolve, although they may swell to some degree. The polymeric ammonium salts described herein, when not crosslinked, are generally soluble in water or other polar solvents. When crosslinked, the polymeric ammonium salts swell in water, often to form gel-like materials and exhibit a swell factor of at least about 2.
Crosslinked polymeric ammonium salts according to the inventive concept(s) described herein will be composed of hydrocarbylene segments, which can contain from 2 to 50 carbon atoms, and are connected by ionic ammonium species. It is believed that the length of the connecting hydrocarbylene segments should match closely with the length of the target PFAS molecules to attain maximum efficiency of absorption. By matching polymeric chain lengths to PFAS length, and with the presence of ammonium ions, the amphiphilic PFAS chains will be provided with maximum opportunity for interactions via both lipophilic (hydrocarbylene chains) and ionic (ammonium ions) modes to maximize the efficacy of absorption of any given PFAS. Additionally, polymer swell will allow the entire mass of the polymer to be accessible to PFAS molecules, further enhancing the efficiency of absorption.
For use as a PFAS sorbent, the crosslinked polymeric ammonium salts described and claimed herein may be used in dry or nearly dry form or swollen in water. It is preferred if the polymeric ammonium salt used has a swell factor of at least about 2, preferably about 5 to 25 and more preferably about 10 to 15 because polymer swell will allow full access of the target PFAS molecules to the entire mass of polymer for very efficient use of all active sites within the polymer. Swell will vary with the conditions employed. A higher swell may be preferred for more stagnant (decanting) situations; whereas a lower swell may be preferred for column type situations, which require lower pressure conditions for processing. Swell factor is a value which corresponds to the ratio of the weight of water imbibed by the polymer divided by the weight of the dry polymer used. It is believed that crosslinked polymeric ammonium salts that swell to the preferred levels have certain advantages for use in dynamic flow situations in towers due to pressure requirements.
The term “ionomer” is used herein to mean a chemical structure having a nitrogen atom bonded to four other atoms. For example, in an ammonium ion, the nitrogen is bonded to four hydrogen atoms. In a primary ammonium ion, the nitrogen atom is bonded to three hydrogen atoms and one carbon atom. In a secondary ammonium ion, the nitrogen atom is bonded to two carbon atoms and two hydrogen atoms. In a tertiary ammonium ion, the nitrogen atom is bonded to three carbon atoms and one hydrogen atom. Finally, in a quaternary ammonium ion, the nitrogen atom is bonded to four carbon atoms.
In the cross-linked polymeric ammonium salts according to the inventive concept(s) described herein, at least 25% of the ammonium nitrogen atoms are secondary ammonium nitrogen atoms, preferably at least about 40% because secondary ammonium nitrogen atoms are associated with linear polymer segments, which reflect how well the polymer swells. A lower percentage of these nitrogen atoms will provide a low swelling polymer, and a higher number of these nitrogen atoms will be associated with polymer that either swells excessively or is predominantly soluble.
According to another embodiment, primary ammonium nitrogen atoms comprise 15% to 25%, secondary ammonium nitrogen atoms comprise 40% to 60%, tertiary ammonium nitrogen atoms comprise 15% to 25%, and quaternary ammonium nitrogen atoms comprise less than 5%, of the total number of ammonium nitrogen atoms in the sorbent polymer.
Each nitrogen atom of the ionomer has one positive charge, and a corresponding counter ion. The counter ion may be any negative ion whose conjugate (Bronsted) acid is capable of protonating the conjugate base of the ammonium salt. Different counterions will provide different levels of hygroscopicity. Suitable compatible counterions include, by way of example, chloride, bromide, iodide, sulfate, phosphate, acetate, ascorbate, carbonate, bicarbonate, nicotinate, salicylate, tartrate and citrate Chloride ion is an especially preferred counterion due to its low molecular weight and environmental safety.
The nitrogen atoms of the ammonium salts (ions) of the polymer are located between polymer segments, unless they are end groups. At least about 25% of these groups, designated herein as Y, linking the nitrogen atoms are independently selected from n-alkylene groups having 2 to about 20 carbon atoms. The term “n-alkylene group” is used herein to mean the group —(CH2)b— wherein the value of b is from 2 to about 20. The n-alkylene group Y may also be substituted with alkyl groups, whereby it is a branched alkylene group. Hydrocarbylene groups of varying lengths may be used and preferred, depending on the target length of the PFAS molecules targeted for absorption/removal.
The other nitrogen atoms of the cross-linked polymeric ammonium salts according to the inventive concept(s) described herein are connected by hydrocarbylene groups, designated herein as Z, containing 2 or more carbon atoms, preferably 2 to 50 carbon atoms, which may be contained in branched and/or cyclic structures, e.g., at least two carbon atoms are positioned between the nitrogen atoms. The term “hydrocarbylene” is used herein to mean a divalent radical, which contains only carbon and hydrogen. The hydrocarbylene group Z may be substituted by various substituents. Contemplated substituents include, by way of example, ether, ester amino, thioether, keto, silyl group and/or heterocyclic rings. It is preferred if the hydrocarbylene group Z is an n-alkylene group containing 2 to 14 carbon atoms in order to fully maximize interactions with linear PFAS molecules. It is also preferred if the substituents contain 1 to 50 carbon atoms, more preferably 1-30 carbon atoms to allow more efficient polymer swell.
One method of preparing the cross-linked polymeric ammonium salts according to the inventive concept(s) described herein is by reacting an organic dihalide with a diamine, both of whose amine groups are primary amines. For the purposes of this disclosure, the organic dihalide can be represented by X—Y—X and/or X—Z—X, where X is chlorine, bromine or iodine (bromine is preferred due to its reactivity with aliphatic diamines), and Y or Z is the group to which both halogen atoms are bound.
The diamine is represented by H2N—Y—NH2 and/or H2N—Z—NH2, where Y or Z is the group to which the two amino groups are bound. In order to obtain the desired polymer, at least some of the dihalide and/or some of the diamine must contain Y as described above. In order to optimally obtain the desired sorbent polymer, it has been found that the Y or Z group should be of such a size that the halogen atoms are the equivalent of at least 7 or more methylene groups spaced apart, that is to say be separated by 7 methylene groups or spaced an equivalent distance if not separated by methylene groups. It is believed that if this minimum separation of the halogen atoms is not achieved, the dihalide will tend to “back bite” after the first halogen has reacted with an amine, and thereby result in an undesirable cyclic structure Thus, it is often convenient (but not necessary) that the dihalide structure be X—Y—X. Groups Y and Z may be selected independently at each position in a particular polymer.
Dihalides useful according to the described and claimed inventive concept(s) are selected from the group consisting of, for example, 1,10-dibromodecane, 1,12-dibromododecane, 1,8-dibromooctane, 1,18-dibromooctadecane, 1,9-dibromononane,1,7-dibromoheptane, 1,8-dilodooctane, 1,8-dibromo-3-ethyloctane, and 1,9-dibromodecane. Useful diamines include, but are not limited to, ethylene diamine, 1,6-diaminohexane, 1,12-diaminododecane, 2-methyl-1,5-diaminopentane, 1,4-bis(aminomethyl)cyclohexane, 1,3-diaminopentane, diethylene triamine, triethylene tetramine, 1,4-bis(3-aminopropyl)piperazine, 1,4-cyclohexanediamine, 5-amino-1-aminomethy1-1,3,3-trimethylcyclohexane, 1,3-propanediamine, 1,4-butanediamine, 1,5-pentanediamine, 1,7-heptanediamine, 1,8-diaminooctane, 1,9-diaminononane, 1,10-diaminodecane, 1,11-diaminoundecane, 2-hydroxy-1,3-propanediamine, and 4,4′-methylene-bis(cyclohexylamine). More than one diamine and/or dihalide may be used in the reaction, so long as the limitations imposed on the polymeric structure are met, for example, at least about 25% of the total groups Y and Z should be Y.
Cross-linked polymeric ammonium salts according to the inventive concept(s) can also be made by reacting a diamine with a di-epoxide. In this case, it is the diamine in which the nitrogen atoms are connected by an n-alkylene group (which may be alkyl substituted) containing 2 to about 20 carbon atoms. After synthesis of these polymers, the resulting amines are converted to ammonium salts by introducing an acid into the reaction.
The cross-linked polyamines (and their salts), as described herein, may have nitrogen atoms that are further substituted, typically by reaction with substituted or unsubstituted alkyl halides to form, for example, secondary amine(salts) from primary amines, and tertiary amines from secondary amines. However, in the resulting polyamine (salt), 25% or more of the amino (ammonium) nitrogen atoms should still be secondary to allow desirable swell properties. The group Q, which is further substituted on a nitrogen, is a hydrocarbyl group containing 1 to 50 carbon atoms, and may contain one or more other substituents selected from the group consisting of hydroxy, ether, amino, thioether, keto, silyl groups and/or heterocyclic rings. It is preferred if Q contains 1-30 carbon atoms to promote desirable swell properties.
The cross-linked polymeric ammonium salts can be made from the above described diamines and dihalides or di-epoxides by dissolving the reactants in a solvent, typically a polar solvent, such as, for example, methanol, ethanol, N,N-dimethylformamide, N,N-dimethylacetamide, dimethylsulfoxide, tetrahydrofuran, water, and mixtures thereof. Temperatures are not critical, so that temperatures in the range of from ambient up to the boiling points of the solvent (or lower boiling ingredient) will produce satisfactory results. Depending on the temperature, reactants and solvent, reaction is complete in a few minutes to a few days, but typically from about 1 to 8 hours. The reaction may be followed by observing the viscosity of the solution, which will gradually increase until a gel is formed or the polymeric product precipitates. If the polymer does not precipitate (whereby it can be isolated by filtration), the polymer can be recovered from the resulting solution by adding the solution to a solvent in which the linear polymer would not be soluble. For example, tetrahydrofuran can be used as a solvent, and the polymer will precipitate.
It is desirable in this process to use approximately equimolar amounts of the diamine and dihalide. The process is preferably carried out under an inert gas blanket to avoid undesired oxidation of the amines. If it is desired to change the counter ion of the polymer, this can be accomplished by adding a solvent (e.g., water) to swell the gel, adding a base, such as ammonium hydroxide or sodium hydroxide (NaOH), to form a salt with the original counterion (and de-swell the gel), removing the salt by filtration and washing, and then re-acidifying with the conjugate acid of the counterion desired to reform a swollen gel. Procedures of this type are known to those skilled in the art.
In processes for preparing the cross-linked polymeric ammonium salts according to the inventive concept(s) described herein, a small amount of the reactants may be polymeric, but not crosslinked. If it is desired to remove the un-crosslinked (and therefore soluble) fraction, removal can be accomplished by extracting the polymeric ammonium salt with a solvent in which the un-crosslinked polymer dissolves, such as, for example, water or methanol (MeOH). See for instance Example 1. Cross-link density (as measured by a polymer's swell factor in water) can be controlled by judicious use of solvents, temperature and reaction time. Some solvents (e g. H2O, EtOH), when used alone, produce polymers that swell very little in water. Mixtures of solvents, and solvents such as MeOH, can produce highly swellable polymers. Short reaction times and/or lower temperatures produce less crosslinking and a higher degree of swelling.
Cross-linking can also be accomplished by using small amounts of tri- or higher functionality amines or halides. Cross-linking can also be accomplished by exposing the uncross-linked polymeric ammonium salt to ionizing radiation.
In the embodiment described above, the cross-linked polymeric ammonium salt should preferably have a swell factor of at least about 2 to 4 in water which will allow target PFAS molecules full access to the mass of the polymer via adequate swell. The degree of swellability of the polymer can be determined by three material factors. One factor is the degree of salt formation in the polymer, that is, what percentage of the amino nitrogen atoms present are in their salt form. The higher this percentage, the more the polymer will swell. It is preferred if at least 80% of the amino groups are in their salt form, and more preferred if at least about 90% are in the salt form for improved swellability. Use of the term “cross-linked polymeric ammonium salt” is intended to include a polymer wherein at least about 50% of the amino groups in the polymer are in their salt form.
Another factor which can influence or control swellability is the hydrophilicity of the groups between nitrogen atoms. Generally, the more carbon atoms these groups contain, the less hydrophilic they are, and the less the polymer will swell in water. Another controlling factor is crosslink density. Typically, higher crosslink density will produce polymer that will swell less.
Reaction conditions during polymer synthesis and handling affect the swell factor. Thus, swell increases with decreasing monomer concentration in the reaction solution, undergoing a sharp increase at high dilution. The reaction time is also important. The reactants react to form a higher molecular weight and more crosslinked polymer at longer incubation times. Reaction temperature contributes to molecular weight growth, with elevated reaction temperatures producing polymers with higher molecular weight (more crosslinks) in shorter periods of time. The workup procedure can also remove low molecular weight polymer and decreases swell. Washing the product with aqueous base, then with acid, shrinks and re-swells the polymer, squeezing out soluble components. A further reduction in swell is observed after continuously extracting the polymer with an organic solvent, followed by water, in a Soxhlet apparatus.
The choice of solvent for the polymerization can have a material effect on the swellability of the final product. A swell factor of essentially zero is obtained in media which do not allow the reactants to dissolve. Swell factor is very low in interfacial systems in which dibromodecane is dissolved in an organic phase and hexamethylenediamine in water. The swell factor can be increased slightly by neutralizing the acid by-product which is generated. Formation of higher swell polymers is promoted by solvents, which dissolve both reactants, especially dipolar, aprotic solvents.
The examples which follow will explain in more detail the various embodiments of the inventive concept(s) described and claimed herein.
Example 1: PFAS absorption as a function of PFAS chain length with 100 mg of poly(alkylamine) ammonium salt (designated HG-1).
A sample of poly(alkylamine) ammonium salt (HG-1) was prepared from hexamethylene diamine and 1,10-dibromodecane, using DMF/methanol as the solvent, according to the method described in U.S. Pat. No. 5,633,344. The sample was tested in a mix of 18 PFAS compounds listed in Table 1 that are prescribed in the drinking water test by the U.S. Environmental Protection Agency (EPA) in method 537.1. Method 537.1 is a solid phase extraction (SPE) liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of selected per- and polyfluorinated alkyl substances (PFAS) in drinking water.
30 mL of tap water and 0.2 mL of 40 ng/mL of EPA 537.1 mixed standard was added to 100 mg of cross-linked polymeric ammonium salt (HG-1) in a polypropylene (PP) 50-mL tube. 30 mL of tap water+0.2 mL of 40 ng/mL EPA 537.1 mixed standard was made up as a control. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 and 4 hours and were then centrifuged at 10,000 rpm for 2 minutes. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol.
Example 2: Experiment with 50 mg of Poly(alkylamine) ammonium salt (HG-1)
30 mL of tap water and 0.2 mL of 40 ng/mL EPA 537.1 mixed standard was added to 50 mg of hydrogel (HG-1) in a PP 50-mL tube. 30 mL tap water+0.2 mL of 40 ng/mL EPA 537.1 mixed standard was made up as a control. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 & 4 hours and centrifuged at 10,000 rpm for 2 min. The samples were then analyzed by LC-MS/MS vs a standard made up in methanol. Results in
Example 3: Experiment with 20 mg of HG-1 in tap water spiked with a mix of PFAS in EPA method 533 which contains more shorter chain PFAS than in method 537.1, as well as Fluoro telomer alcohol PFAS of the type used in aqueous fire-fighting foams.
30 mL of tap water and 0.2 mL of 40 ng/mL of a mixed PFAS standard used in EPA method 533 was added to 20 mg of hydrogel (HG-1) in a PP 50-mL tube. The mix of PFAS in EPA method 533 contained more shorter chain PFAS than specified in EPA method 537.1, as well as a few Fluoro telomer alcohols. The mix of analytes in 533 is shown in Table 2.
30 mL tap water+0.2 mL of 40 ng/mL EPA method 533 mixed standard was made up as a control. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 and 4 hours and then centrifuged at 10,000 rpm for 2 min. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol.
30 mL of tap water and 0.2 mL of 40 ng/mL EPA method 533 (Table 2) mixed standard was added to 20 mg of hydrogel (HG-1) in a PP 50-mL tube. 30 mL tap water+0.2 mL of 40 ng/mL EPA 537.1 mixed standard was made up as a control. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 & 4 hrs. They were allowed to settle and then centrifuged at 10,000 rpm for 2 min. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol.
Results of the control experiment with no HG-1 are shown in
Example 4: PFAS absorption as a function of PFAS chain length in EPA standard method 533 with 10 mg of Poly(alkylamine) ammonium salt (HG-1). 30 mL of tap water and 0.2 mL of 40 ng/mL EPA method 533 (Table 2) mixed standard was added to 10 mg of hydrogel (HG-1) in a PP 50-mL tube. 30 mL tap water+0.2 mL of 40 ng/mL EPA 537.1 mixed standard was made up as a control. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 and 4 hours. They were allowed to settle and centrifuged at 10,000 rpm for 2 min. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol.
Tests in Well Water
Example 5: A test was conducted with HG-1 in well water taken from the vicinity of an airport and air national guard site with a history of AFFF use. The well water (Well 1) contained much higher amounts of PFAS than in the experiments using tap water listed above. The well water also contained a different mix of PFAS as shown in Table 3, as well as other organic matter, such as Humic acid.
30 mL of well water (Table 3) with no HG-1 was added to a PP 50-ml tube. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1 & 2 hours. They were allowed to settle and centrifuged at 10,000 rpm for 2 minutes. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol.
Results shown in
To test the efficacy of HG-1 to remove PFAS from well water, 30 mL of well water (Table 3) with 50 mg of HG-1 was added to a PP 50-ml tube. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1 & 2 hours. They were allowed to settle and then centrifuged at 10,000 rpm for 2 minutes. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol. Results in
Example 6: Test of 20 mg of HG-1 in well water (Well 2) in the vicinity of an airport and air national guard site with a history of AFFF use.
30 mL of well water from a well (Well 2) that had a higher level of PFAS contamination than in Example 5 was added to 20 mg of HG-1 in a PP 50-ml tube. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 and 4 hours. They were allowed to settle and then centrifuged at 10,000 rpm for 2 minutes. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol. Results in
Example 7: Test of 10 mg of HG-1 in well water (Well 2) from the vicinity of an airport and air national guard site with a history of AFFF use.
30 mL of well water from Well 2 was added to 10 mg of HG-1 in a PP 50-ml tube. All samples were put on a wrist-action shaker. 1-mL aliquots were taken after 1, 2 and 4 hours. They were allowed to settle and then centrifuged at 10,000 rpm for 2 minutes. The samples were then analyzed by LC-MS/MS vs. a standard made up in methanol. Results shown in
Results in
Experiments to Demonstrate Release of PFAS from Sorbent (HG-1) and Recovery of PFAS from Sorbent
Example 8: PFAS release (i.e., desorption, recovery) from HG-1 was demonstrated using samples from Example 2 (50 mg of HG-1 in tap water spiked with a mix of PFAS in EPA method 537.1). After the absorption experiment was completed, the remaining water was removed from the settled hydrogel by aspiration. Then HG-1 was treated with 30 mL of 2% ammonium hydroxide in methanol. After one hour of shaking, the samples were centrifuged, and an aliquot of the ammonium hydroxide/methanol solution was evaporated, reconstituted in methanol and then analyzed by HPLC/Mass spectrometry.
Example 9: Results of an experiment to demonstrate absorption of PFAS by HG-1 (samples from experiment 5 with 50 mg) in water from well water contaminated with PFAS followed by desorption. After the absorption experiment was completed, the remaining water was removed from the settled hydrogel by aspiration. Then HG-1 was treated with 30 mL of 2% ammonium hydroxide in methanol. After one hour of shaking, the samples were centrifuged, and an aliquot of the ammonium hydroxide/methanol solution was evaporated, reconstituted in methanol and then analyzed by HPLC/Mass spectrometry.
Example 10: Product Made by Alternate Synthetic Methods
Experiments were conducted to test efficacy of absorption and desorption of products made by alternate synthetic methods. Two polymer samples, designated HG-2 and HG-3, were subject to absorption and desorption experiments in well water as described previously. HG-2 and HG-3 were prepared using the same ingredients as were used in preparing HG-1, but with THF as solvent.
Results in
Example 11—Sorption experiment to assess the effect of increasing the cross-link density of the ammonium salts on PFAS sorption
Ammonium salts (i.e., ionomers) were synthesized following the procedure described in U.S. Pat. No. 5,633,344 using different molar ratios of dibromodecane and hexamethylene diamine monomers ranging from equimolar amounts of the two monomers, and 4% and 6.3% molar excess of the dibromodecane to vary the cross-link density of the ionomer sorbent.
For each sorbent to be studied, 10 mg of each was weighed out into a 50-mL polypropylene centrifuge tube (each tube is referred to herein as a reactor). 30 mL of HPLC-grade water was added to each reactor, followed by 0.3 mL of 10 ng/mL EPA 533 PFAS mixture. Each reactor was shaken by hand for 15 seconds and the contents were allowed to settle for 5 minutes. After settling, 0.5-mL samples were taken by pipette and placed into 2 mL microcentrifuge tubes that contained 0.5 mL methanol and internal standard. Samples were centrifuged for 2 minutes at 10,000 rpm. All reactors were then placed on a wrist-action shaker for 1 hour. After 2 hours, reactor contents were allowed to settle for 5 minutes, and a sample was collected using the same methods described for the first sample. These samples were analyzed by LC-MS/MS vs. a standard in methanol solution.
Example 12—Experiment to compare the performance of poly(alkylamine) ammonium salt (HG-1) to remove PFAS from well water contaminated by PFAS from aqueous fire-fighting foam, against Granular Activated Carbon (GAC) and Ion Exchange Resin (IAX)
Well water from a well near an Air National Guard site, which was tested by LC/MS/MS and shown to contain PFAS compounds, was used for this experiment. The amounts of PFAS in the well water are shown in Table 3 below.
200 mg of poly(alkylamine) ammonium salt (HG-1) was weighed into a polypropylene beaker and then 200 mL HPLC-grade water was added. The HG-1 and water solution was mixed for 30 minutes prior to taking 1 mL of solution to obtain 1 mg/mL of HG-1 ionomer while stirring. This 1 ml of HG-1 ionomer solution was added to a 125 mL PP bottle containing 99 mL of well water. For comparative purposes, 40 mg of HG-1 ionomer, GAC, and IAX were weighed into 125-mL PP bottles containing 100 mL of well water. The bottles were shaken by hand for 10 seconds and their contents allowed to settle for 1 minute. The bottles were then sampled by pipetting 1 mL into a microcentrifuge tube containing an internal standard. The microcentrifuge tubes were centrifuged for 2 minutes at 10,000 rpm, and then 0.9 mL of centrifuged sample was pipetted for analysis. The bottles were then placed on a wrist-action shaker for 1 hour. After 1 hour, another sample was taken from the bottles, in the methods described above. These samples were analyzed by LC-MS/MS vs. a standard in methanol solution.
Example 13—Performance of poly(alkylamine) ammonium salt, wherein the poly(alkylamine) ammonium salt was prepared from polyethylene imine and dibromodecane using DMF/methanol as solvent (designated HG-5) according to the procedure described in U.S. Pat. No. 5,633,344, against GAC and IAX to remove perfluoro-octanoic acid (PFOA)
200 mg HG-5 was placed in a polypropylene beaker, and 200 mL HPLC-grade water was then added to form a solution. The solution was mixed for 30 minutes prior to removing 1 mL for the study. A control reactor was created by mixing 100 mL HPLC-grade water with 0.1 mL 1 ug/mL perfluoro-octanoic acid (PFOA) solution in a 125-mL polypropylene bottle. For GAC and IAX, 8 mg of each was weighed into 125 mL bottles and 100 mL water and 0.1 mL 1 ug/mL PFOA solution were added. For HG-5, 1 mL of sorbent slurry was added to a bottle containing 99 mL water and 0.1 mL 1 ug/mL PFOA solution. The bottle reactors were shaken by hand for 10 seconds and the contents allowed to settle for 1 minute before sampling. After settling, 1 mL of solution was pipetted from each bottle into microcentrifuge tubes, centrifuged for 2 minutes at 10,000 rpm, and then 0.9 mL of each sample was analyzed by HPLC-MS/MS. The first sample was the time 0 sample. Bottles were placed on a wrist-action shaker and sampled as described above at time intervals of 1, 2, 4, and 6 hours.
Example 14—Experiment to test the performance of HG-1 and HG-5 to absorb PFOA at high concentrations and compare the result against the performance of GAC and IAX to absorb PFOA at high concentrations
8 mg of HG-1, HG-5, GAC, and anion exchange resin were all weighed into separate 20 mL polypropylene bottles. 16 mL HPLC-grade water was added to each bottle, followed by 4 mL of 1 μg/mL PFOA solution. Controls consisted of 16 mL water and 4 mL PFOA solution. All bottles were shaken by hand for 10 seconds and their contents allowed to settle for 1 minute. Samples of 25 μL were taken from each bottle and combined in microcentrifuge tubes with 975 μL water, centrifuged, and aliquots were taken for HPLC-MS/MS analysis. Bottles were placed on a wrist-action shaker and sampled by the above-mentioned methods at the following time intervals: 1, 2, 4, 6, and 24 hours. All samples were analyzed by HPLC-MS/MS.
Example 15—Poly(alkylamine) ammonium Salt Samples HG-1 and HG-5 Sorption Efficacy at Low PFOA Concentrations.
Poly(alkylamine) ammonium salt samples HG-1 and HG-5 capabilities were compared for removal of PFOA from aqueous slurries at low concentrations. The samples were tested by the following method. Sorbent slurries were prepared to achieve a more accurate 1 mg mass of sorbent. 200 mg quantities of each sorbent were added to polypropylene beakers and mixed with 200 mL HPLC-grade water. Sorbent slurries were mixed for 30 minutes prior to pipetting 1 mL of slurry solution into 125-mL polypropylene bottles which contained 99 mL water and 0.1 mL of 1 ug/mL PFOA solution. A control bottle was also prepared which was 100 mL water and 0.1 mL of 1 ug/mL PFOA solution. Samples were taken from each bottle and pipetted into microcentrifuge tubes, centrifuged, and aliquots were taken for HPLC-MS/MS analysis. The bottles were placed on a wrist-action shaker and sampled by the above-described methods at the following time intervals: 1, 2, 4, 6, and 24 hours. All samples were analyzed by HPLC-MS/MS.
Example 16—Experiments to demonstrate use of cross-linked poly(alkylamine) ammonium salts under dynamic flow conditions by passing water through a dry-packed column.
Column studies were performed for 10 mg quantities of sorbent samples HG-1 and HG-5 dry packed into 1 mL columns to demonstrate PFAS removal from PFAS-contaminated tap water. A bottom filter frit was placed in the 1 mL columns. 10 mg of each sorbent was weighed into separate columns. Top filter frits were added a distance above the sorbent to account for sorbent expansion. The columns were placed on a vacuum pump apparatus, and 1 mL of HPLC-grade water was added to each column until the sorbent samples were saturated. Enough vacuum pressure was applied to pull water through the columns. The top filter frits were pressed into each column until the frits touched the surface of the sorbent samples. Following that, 100 mL of tap water was passed through each column. Filtrate was collected below each column. Solid phase extraction was performed on the filtrate as well as the tap water as a control by the EPA 533 methods and then analyzed by HPLC-MS/MS.
Results for one dry-packed column study are shown in
Example 17—Column Flow Test for PFOA Sorption using a 1:9 ratio of Sorbent to Granulated Activated Carbon (GAC).
A mass ratio of 1:9, sorbent to GAC, was performed for a column study of PFOA sorption. 30 mg of each sorbent was combined with 270 mg GAC and mixed by glass mortar and pestle until relatively homogenous. 100 mg of each sorbent/GAC mixture was added to columns with bottom column frits already inserted. 100 mg of only GAC was also weighed into the columns. 20 mL of PFOA solutions were made by adding 50 μL of 1 μg/mL PFOA solution to 9.95 mL of water. Controls were made with 10 mL of water. Columns were then placed on a vacuum apparatus and 10 mL HPLC-grade water was passed through each column without top frits inserted. Sorbents were allowed to become completely saturated with the HPLC-grade water, and then top filter frits were added to the columns and pressed firmly against the sorbent/GAC mixtures so as to press out air pockets without compacting the material. Reservoirs were added to each column to hold approximately 4 mL of solution. All columns were assembled in duplicate so that PFOA solutions and a water blank were passed through each sorbent mixture or GAC type. Filtrate was collected below the columns in a 15 mL centrifuge tube. Each solution was pulled through vacuum very slowly, allowing a slow single drop to pass through at a time. Collected filtrate was vortexed and a 1 mL sample of each was taken and analyzed by LC-MS/MS.
The results from Example 17 are shown in
Example 18—Experiments to identify conditions under which PFAS compounds absorbed by the poly(alkylamine) ammonium salts according to the inventive concept(s) described and claimed herein can be recovered from the salts.
Absorption and desorption (i.e., removal & recovery) of PFAS compounds was tested for poly(alkylamine) ammonium salt samples HG-1 and HG-5 using different alkaline solutions. In triplicate, 8 mg of each sorbent HG-1 and HG-5 was weighed into 20 mL polypropylene bottles. 4 mL of 1 μg/mL PFOA compounds and 16 mL HPLC-grade water was added to each reactor, i.e., each bottle. The bottles were shaken by hand for 15 seconds and then allowed to settle for 1 minute. Samples from each bottle were taken, centrifuged at 10,000 rpm for 2 minutes, and aliquots saved for analysis. The bottles were then placed on a shaker table for 2 hours. At 2 hours, samples were taken from each bottle and processed as above. Bottles were allowed to settle for 5 minutes and then solution was carefully removed by disposable pipette without removing sorbent from the bottom of the bottles. 20 mL of three different desorption solutions were then added to one of each sorbent type bottle. The desorption solutions were as follows: (1) 2% ammonium hydroxide in methanol, (2) 2% ammonium hydroxide in water, and (3) 2% sodium hydroxide in water. Preferred pH range for all desorption solutions based on observed results is from about 8 to 14. The bottles were shaken on a shaker table for 1 hour and were then allowed to settle. 15 mL of solution was removed from the two bottles that contained ammonium hydroxide in methanol, and the solutions were evaporated to dryness by a gentle stream of nitrogen. Dry samples were reconstituted to 1 mL and processed by HPLC-MS/MS. The bottles containing water solutions were processed by solid phase extraction, following the EPA 533 method for concentrating PFAS in solution. Care was taken not to pass sorbent material into the solid phase extraction by careful pipetting. Some solution remained at the bottom of the bottle so as to avoid processing the sorbent material. Samples were then processed by HPLC-MS/MS. This same process was also completed for both 2% ammonium and sodium carbonate aqueous solutions and the resulting data has been added to the hydroxide desorption figure (
The cross-linked ammonium salts described herein can be used in passive sampling devices, such as, for example, cartridges and discs, for monitoring PFAS contaminants in water. They can also be used in polar organic chemical integrative samplers (POCIS). Such samplers are a passive sampling device which allows for the in situ collection of a time-integrated average of hydrophilic organic contaminants. POCIS provides a means for estimating the toxicological significance of waterborne contaminants. The POCIS sampler mimics the respiratory exposure of organisms living in the aquatic environment and can provide an understanding of bioavailable contaminants present in the system. POCIS can be deployed in a wide range of aquatic environments and is commonly used to assist in environmental monitoring studies.
Most aquatic monitoring programs rely on collecting individual samples, often called “grab samples”, at a specific time. The grab sampling method has many disadvantages, some of which can be resolved by passive sampling techniques. When contaminants are present in trace amounts, grab sampling may require the collection of large volumes of water. Also, lab analysis of the grab sample can only provide a snapshot of contaminant levels at the time of sample collection. This approach, therefore, has drawbacks when monitoring pollutants in environments where contamination varies over time and episodic contamination events occur.
The cross-linked ammonium salts described herein, due to their capability to absorb PFAS rapidly, can provide an effective way to monitor PFAS contamination. Devices, such as cartridges, which contain the cross-linked ammonium salts, can be placed in monitoring wells, and after a predetermined length of time, the devices can be removed and subject to conditions to desorb the PFAS compounds according to the inventive concept(s) described herein. The freed PFAS compounds can then be analyzed and quantified. Such systems which contain cross-linked ammonium salts enable study of the speed with which a plume of PFAS pollution moves in ground water and pore water pollution and the direction in which it moves. This type of information reduces uncertainty and provides essential information before, during, and after soil remediation and contamination management processes. Passive samplers which contain cross linked ammonium salts can be deployed in a wide range of aquatic environments, including, for example, stagnant pools, wells, rivers, springs, estuarine systems, and wastewater streams.
The cross-linked ammonium salts according to the inventive concept(s) described herein can also be used to rapidly absorb PFAS in spills, such as, for example, in spent aqueous liquid that results from use of PFAS of the type contained in fire-fighting foam, or in industrial and municipal waste water streams and systems which have PFAS contaminants.
As those skilled in the art will appreciate, numerous modifications and variations of the described and claimed inventive concept(s) are possible in light of these teachings, and all such are contemplated hereby. The present invention contemplates and claims those inventions that may result from the combination of features described herein and those of the cited prior art references which complement the features of the present invention.
| Number | Date | Country | |
|---|---|---|---|
| 63081129 | Sep 2020 | US |
