Patent Application
20150140097
The present invention relates to a composition comprising a plurality of microspheres, wherein the microspheres are perfectly spherical, and the method of manufacturing the same. The present invention is useful in the manufacture of sustained and modified release active pharmaceutical ingredient (API) microspheres, as a free flowing excipient for mini-tablets and in the manufacture of API carrier dispersions.
Many commercial pharmaceutical beads are either reactive or insoluble. Reactive beads such as sucrose/starch beads can cause incompatibility with active substances and loss of active substance due to the presence of reducing sugars. Reaction of moisture in beads made with microcrystalline cellulose, sucrose, starch or cellulose derivatives containing beads can cause incompatibility with active substances and loss of active substance due to the presence of moisture. Loss of API in insoluble beads such as those made with microcrystalline cellulose, starch or cellulose derivatives can result in lack of release of active substance or lower extraction yields from the insoluble materials due to the of insoluble matrixes. Beads made with soluble components such as polyols can be made with very low moisture content (anhydrous) and can be made completely soluble.
Current polyol beads are granulated, thus undissolved polyol particles, primary particles, are “glued” together with a binder solution to make a secondary granular structure. This process makes a surface that is only as smooth and durable as the starting particle size and as the shape will allow. The starting material is not completely liquefied as some remain solid in the granulation route approach and thus transitions are present. Also for very small spheres the contour of the starting particle contributes to a lack of having a smooth crevice-free and bump-free surface, thus lacking perfectly shaped solid spheres. Because the binder contains a solvent, the wet beads must be dried. Bead drying can create internal porosity as well as transition layers of insoluble materials between the undissolved bodies we are calling primary particles. Formation of a wet mass is often done using a granulator, followed by an extruder to form a dense packed pellet and then spinning the pellet on a friction plate into a sphere. Formation can also be done by a powder layering process on a core particle or bead that needs to be large enough to maintain separation in the coating process. This required core and the need to maintain separation restricts the size of the bead that can be made. The layering process starts with seed core upon which insoluble primary particles are deposited and bonded using the binder solution. For effective layering the primary particles must be small enough (<10 μm for 150 μm sphere) to be formed into a reasonably smooth surfaced sphere (<30 μm for 300 μm sphere). The primary layering particles and the layer application amount must be small enough to prevent porosity and/or moisture from being trapped deep in the sphere. Drying during layering process is critical to balance enough wetness for growth, bead strength and dryness for reduced interior moisture and prevent vacuoles/residual porosity. A water insoluble wicking agent such as MCC aides in the removal of moisture but is insoluble. Final bead size is limited to spheres larger than 100 μm mean size (10 μm primary layering particle size) to allow granular shaping and maintain bead separation (preventing twins) during the layering process.
Commercially available beads used as cores as API delivery beads in applications that can survive the temperature/tumbling conditions of the API coating and layering process are larger than 100 μm (mean particle diameter). Tablets containing API delivery beads incorporated and compressed into tablets require smaller size beads if bead crushing/rupturing of the functional coating on coated bead during tablet compression is to be avoided. Tablets containing beads are made typically into swallow tablets to avoid chewing, thus tablet thickness needs to be small to allow ease of swallowing. Beads need to be cushioned during tablet compression to prevent them from being crushed with larger bead requiring more cushioning materials. Larger beads place limitations on the tableting process (slower press speed) and formulation (requires more crushing agents) to create an environment that prevents bead fracturing of larger beads. Smaller beads thus allow for smaller tablets, less cushioning ingredients to be required in tablet formulation as well as in the coating layers and higher dose loading of API.
Excipients for very small mini-tablets (<3 mm in tablet diameter), require very small excipient particles for fill/tablet weight control. A 1/50 of the diameter of the tablet standard for particle size would require a particle size mean of 60 μm. Current<90 μm particles of microcrystalline cellulose (MCC) (and milled MCC<90 μm) are used. These materials are not spherical and thus prone to flow issues causing weight uniformity issues, especially at faster tablet press speeds.
In one embodiment, the present invention provides for improved microspheres that can be 100% soluble, perfectly spherical, have a uniform surface with limited <2 micron peak to valley roughness, be as small as 2 μm, be comprised in some embodiments of a single crystal structure with limited or no internal voids, have low hygroscopicity, and low moisture content of less than 1% weight percentage.
In one embodiment, the present invention relates to improved microspheres comprising a core material, wherein the microsphere is perfectly spherical, and the method of manufacturing the same. In some embodiments, the present invention relates to improved microspheres comprising a core material, wherein the microsphere is perfectly spherical and has a moisture content less than 1% by weight. In some embodiments, the present invention relates to improved pharmaceutical microspheres comprising a core material, wherein the microsphere is perfectly spherical, has a smooth surface and has a moisture content less than 1% by weight.
In some embodiments, the present invention relates to improved microspheres comprising a core material, wherein the microsphere is perfectly spherical, has low hygroscopicity, and has a moisture content less than 1% by weight.
The foregoing summary, as well as the following detailed description of the invention, will be better understood when read in conjunction with the appended drawings. For the purpose of illustration the invention, there are shown in the drawings embodiments which are presently preferred. It should be understood, however, that the invention can be embodied in different forms and thus should not be construed as being limited to the embodiments set forth herein.
The following detailed description is exemplary and explanatory and is intended to provide further explanation of the invention described herein. Other advantages, and novel features will be readily apparent to those skilled in the art from the following detailed description of the invention.
The present invention is described herein using several definitions, as set forth below and throughout the application.
The term “about” will be understood by persons of ordinary skill in the art and will vary to some extent on the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art given the context in which it is used, “about” shall mean up to plus or minus 10% of the particular value.
The terms “solid dosage form,” “tablet,” and “solid preparation” are used synonymously within the context of the present invention. These terms should be construed to include a compacted or compressed powder composition obtained by compressing or otherwise forming the composition to form a solid having a defined shape.
The aim of the present invention was to overcome the drawbacks of existing commercial beads.
Microsphere refers to a sphere that is from about 1 μm to about 3 mm. In one embodiment, the present invention relates to a composition comprising a plurality of microspheres. A plurality of microspheres comprises from about 50 to about 20,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 50 to about 15,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 50 to about 10,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 50 to about 5,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 100 to about 20,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 100 to about 15,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 100 to about 10,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 100 to about 5,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 100 to about 1,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 500 to about 20,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 500 to about 15,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 500 to about 10,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 500 to about 5,000 microspheres. In some embodiments, a plurality of microspheres comprises from about 500 to about 1,000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 50 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 100 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 200 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 300 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 400 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 750 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 1000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 1250 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 1500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 1750 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 2000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 2500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 3000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 3500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 4000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 4500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 5000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 7500 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 10,000 microspheres. In some embodiments, a plurality of microspheres comprises greater than about 15,000 microspheres.
In some embodiments, the present invention relates to a composition comprising a plurality of microspheres, wherein the microspheres have perfect sphericity. “Perfect sphericity” or “perfectly spherical” means a circularity as measured by imaging microscopy of greater than about 0.90, and an aspect ratio of less than about 1.0. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.91. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.92. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.93. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.94. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.95. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.96. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.97. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.98. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a circularity greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 40% of microspheres have a circularity greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 50% of microspheres have a circularity greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 60% of microspheres have a circularity greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 70% of microspheres have a circularity greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 20% or less of microspheres have a circularity greater than about 0.98. Circularity is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.90. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.91. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.92. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.93. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.94. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.95. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.96. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.97. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.98. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 1.0. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.90 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.91 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.92 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.93 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.94 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.95 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.96 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.97 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.98 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an aspect ratio of about 0.99 or greater. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 20% of microspheres have an aspect ratio greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 30% of microspheres have an aspect ratio greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 40% of microspheres have an aspect ratio greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 50% of microspheres have an aspect ratio greater than about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 60% of microspheres have an aspect ratio greater than about 0.99. Aspect Ratio is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 30% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 40% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 50% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 60% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 70% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 80% of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 30% or more of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 40% or more of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 50% or more of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 60% or more of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 70% or more of the microspheres have a convexity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% or more of the microspheres have a convexity of about 0.99. Convexity Ratio is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 30% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 40% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 50% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 60% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 70% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 80% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein at least about 90% of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 30% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 40% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 50% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 60% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 70% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% or more of the microspheres have a solidity of about 0.99. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% or more of the microspheres have a solidity of about 0.99. Solidity Ratio is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a mean particle size from about 2 μm to about 3000 μm in diameter. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a mean particle size from about 2 μm to about 10 μm in diameter. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a mean particle size from about 10 μm to about 20 μm in diameter. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a mean particle size from about 10 μm to about 500 μm in diameter. This mean particle size d(0.5) can be anywhere within this range based on process conditions chosen. In one embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution (d(0.9)/d(0.1)) of about 2.8 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 2.7 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 2.4 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 2.3 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 2.2 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 2.1 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a mean particle size distribution of about 2.0 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.9 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.8 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.7 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.6 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.5 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.4 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.3 or less. In another embodiment of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a particle size distribution of about 1.2 or less. In some embodiments, the present invention provides for microspheres with a narrow particle size distribution without a first microsphere size separation step. In some embodiments, the present invention provides for microspheres with a narrow particle size distribution prior to a first microsphere size separation step.
In some embodiments, the perfect sphericity, perfect solidity, narrow particle density and lack of pores of the microspheres of the present invention create the ability to use size selection to narrowly control the effective surface area (ESA) for coating the microspheres per mass. The effective surface area is the surface at the base of the functional film or coating whose thickness for performance is fixed at a minimum and a maximum. Normal for functional films or coating, the thickness required is a film or coating of 10 μm or more. If substantially all of the surface of the microsphere is part of the start of the functional thickness layering it is as if the coating or film is being applied to a flat surface. Thus the build of thickness is uniform and reproducible. Coating lost into pores or needed over risers is not lost if the microsphere has both a high solidity and a high convexity value. The surface area for the weight of beads used in the coating batch can thus be related directly to bead size and bead size frequency even down to 10 μm beads. Factors of shape, bead density, and effective surface lost into crevices and pores and surface distortions from risers are no longer factors that affect the relationship of size selection to the effective surface per batch to be coated. Also particle flow of a circular microsphere at 10 μm is maintained as particles are spherical and particle to particle contact is at points of contact and thus minimal surface area involved. Also minimal moisture <0.2% generates a very small surface free energy at contact points. In some embodiments, microspheres of the present invention also can be made static free as the process is a crystallization process, even at the 10 μm level. Maintaining separation is extremely important to prevent agglomeration during coating and use. Aerodynamics is also uniform based on the microspheres having uniform shape and density. Submicron crystal ridges exist even on the 10 μm microsphere thus even though the microsphere appears smooth the surface can be attached to with coating materials.
In some embodiments, the present invention provides for the manufacture of microspheres that have a standardizable surface area, due to their perfectly spherical shape and their lack of internal porosity. In some embodiments of the present invention, the microsphere lacks internal porosity. In some embodiments, the microsphere lacks internal voids. A void is defined as an area in the bead that is not open to the surface and thus not a pore. This open area normally filled with air causes the density of the particle to be lowered when present. If present, these pores create particle density variability as their presence is usually not uniform. Thus in process using sonic nozzles or using a size selection process a specific narrow range of surface area/process weight used will allow coating a much more exact surface area and the variability of film thickness controlled. Current commercial beads are made and available in size ranges. In some embodiments, the present invention provides for the manufacture and/or sorting of microspheres to provide microspheres of a standard surface and much more narrowed surface area range per weight of beads used per batch for coating process. This provides for a uniformly coated microsphere and narrows the distribution of coating film thicknesses from batch to batch.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a smooth surface. A smooth surface with lack of bumps is essential for uniformity in the coating thickness of an API and decreasing the risk of pin hole formations in the coating film. However small ridges in the microsphere surface aide in the adherence of binder or coating solution to the surface of the microsphere. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres have a surface with ridges of about 4 μm or less in height. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres have a surface with ridges of about 3 μm or less in height. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres have a surface with ridges of about 2 μm or less in height. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres has a surface with ridges of about 1 μm or less in height. In one embodiment, a microsphere has a surface with ridges of about 0.5 μm or less in height. In one embodiment, a microsphere has a surface with ridges of less than about 4 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 3 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 2 μm in height. In one embodiment, a microsphere has a surface with ridges of <1 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.9 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.8 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.7 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.6 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.5 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.4 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.3 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.2 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.1 μm in height. In one embodiment, a microsphere has a surface with ridges of less than about 0.05 μm in height.
In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprising a plurality of microspheres does not have any ridges that exceed about 0.05 μm in height.
In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.9 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.8 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.7 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.6 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.5 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.4 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.3 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.2 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.1 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 80% of the microspheres do not have any ridges that exceed about 0.05 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 85% of the microspheres do not have any ridges that exceed about 0.05 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 90% of the microspheres do not have any ridges that exceed about 0.05 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 95% of the microspheres do not have any ridges that exceed about 0.05 μm in height. In some embodiments, a composition comprises a plurality of microspheres, wherein about 99% of the microspheres do not have any ridges that exceed about 0.05 μm in height.
In some embodiments, a composition comprises a plurality of microspheres, wherein the surface of the microsphere is comprised of flat, crystal plates. In some embodiments, a composition comprises a plurality of microspheres, wherein the surface of the microsphere is comprised of flat, crystal plates that include ridges at one or more portions of a periphery of the plates. In one embodiment, the ridges extend radially from the periphery of the plate away from the center or core of the microsphere. In one embodiment, the ridges extend radially away from the surface to the center or core of the microsphere. In some embodiments, the crystal plates with ridges form shallow ridges of less than about 2 μm in height. This allows a film polymer to grip to during the early stages of coating and creates a surface with minimum loss of film material. In some embodiments, the formation of the flat crystal plates is crystals extending to the surface of the microsphere in stacked flat crystals forms of either that from growth terminations as a stacked group of thin crystals in bundles, each bundle creating a surface plate. In this embodiment, surface ridges are created by crystal plates on the surface that are less than about 1 μm in height. In another embodiment, the flat crystal plates on the microsphere surface form as crystallization layers in an onion layer growth pattern. In some embodiments, the surface ridges occur at a frequency greater than about one per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency greater than about two per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency greater than about three per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency greater than about four per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency greater than about five per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about one per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about two per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about three per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about four per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about five per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about one to about five per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about two to about five per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about three to about five per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of about four to about five per μm distance along the surface of a microsphere.
In some embodiments, the surface ridges occur at a frequency of less than about one per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of less than about two per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of less than about three per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of less than about four per μm distance along the surface of a microsphere. In some embodiments, the surface ridges occur at a frequency of less than about five per μm distance along the surface of a microsphere.
In some embodiments, the flat crystal plates on the surface of the microsphere are horizontal to a surface of the microsphere and are formed either during the droplet formation due to spinning orientation and/or cooling (surface nucleation orientation) of the droplet to form the solid microsphere. In some embodiments, the flat, crystal plates on the surface of the microsphere is formed by the surface of the spinning disc. In some embodiments, the flat, crystal plates on the microsphere surface are horizontal to a surface of the microsphere. This may be based on spin roll. In some embodiments, the flat, crystal plates on the microsphere surface is formed as a molecular orientation on the disc. In some embodiments, the flat, crystal plates on the microsphere surface is formed from the cooling process. In some embodiments, the flat, crystal plates on the surface of the microsphere formed by the surface of the spinning disk is adjustable based on the surface of the spin disc temperature. In some embodiments, the flat, crystal plates on the surface of the microsphere formed by the surface of the spinning disk is adjustable based on the spinning speed. In some embodiments, the flat, crystal plates on the surface of the microsphere formed by the surface of the spinning disk is adjustable based on the surface of the spin disc temperature and the spinning speed. Although the use of a spinning disc is one method of making the microspheres according the inventions described here, the microspheres described herein are not limited to those microspheres resulting from the methods disclosed herein. Indeed, the microspheres according to the inventions described herein can be made by any method that will result in the microspheres according to the inventions described herein.
In contrast, risers, rounded projections from the surface of a microsphere, cause coating thickness variation by generating local spots of thin coating or pin holes. If a functional coat must be 10 μm thick and pin holes need to be avoided then the coating over the riser needs to be 10 μm thick, making the coating in other regions required to be thicker. In some embodiments, the surface of the microspheres of the present invention has uniform ridges which coating materials can grip to hold onto. In some embodiments, these ridges have a less than about 2 μm depth and thus contribute little to coating thickness variability. In some embodiments, a composition comprises a plurality of microspheres, wherein the surface of the microsphere does not have risers.
In some embodiments, a composition comprises a plurality of microspheres, wherein the microsphere has vertical or radially rising, very tightly packed crystal formations that are underneath the surface of the microsphere. In some embodiments, the vertical or radially rising, and very tightly packed crystal formations in the microsphere and the solid center of the microsphere allow the skeletal density of the microsphere to approach the true density reported for alpha mannitol, and allow for a very narrow control of particle density.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density of about 1.4595 to about 1.4651 gm/cc by helium pynometry. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have an average skeletal density of about 1.461 gm/cc. This is compared to the true density by x-ray diffraction of a polyol alpha mannitol crystal form found of 1.468 gm/ml. In some embodiments, this develops a bead porosity of (1−(1.468−1.461)/1.468)*100=˜0%. This crystal lattice density matches the DSC scan for beads showing a match of crystal energy of alpha mannitol and the melting point match to alpha mannitol identifies these beads a solid crystal alpha mannitol forms. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about 10% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −10% and +10% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −9% and +9% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −8% and +8% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −7% and +7% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −6% and +6% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −5% and +5% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −4% and +4% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −3% and +3% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −2% and +2% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −1% and +1% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.9% and +0.9% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.8% and +0.8% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.7% and +0.7% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.6% and +0.6% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.5% and +0.5% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.4% and +0.4% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.3% and +0.3% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.2% and +0.2% of the skeletal density of the microspheres' material. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres have a skeletal density within about −0.1% and +0.1% of the skeletal density of the microspheres' material.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have low hygroscopicity. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres have a moisture gain of about 0.18% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 2.0% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.9% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.8% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.7% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.6% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.5% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.4% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.3% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.2% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.1% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 1.0% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.9% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.8% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.7% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.6% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.5% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.4% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.3% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.2% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.1% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of less than about 0.05% by weight at about 90% relative humidity. In one embodiment, a microsphere has moisture gain of about 0% by weight at about 90% relative humidity.
In another embodiment, a microsphere has a moisture gain of less than about 1.00% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.90% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.80% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.70% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.60% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.50% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.40% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.30% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.20% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.10% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.09% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.08% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.07% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.06% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.05% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.04% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.03% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.02% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of less than about 0.01% by weight at about 60% relative humidity. In another embodiment, a microsphere has a moisture gain of about 0% by weight at about 60% relative humidity. Hygroscopicity is measured by Dynamic Vapor Sorption (DVS).
In some embodiments of the present invention, a microsphere has a moisture content of about 2.0% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.9% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.8% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.7% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.6% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.5% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.4% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.3% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.2% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.1% by weight or less. In one embodiment, a microsphere has a moisture content of about 1.0% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.9% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.8% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.7% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.6% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.5% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.4% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.3% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.2% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.1% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.09% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.08% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.07% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.06% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.05% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.04% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.03% by weight or less. In one embodiment, a microsphere has a moisture content of about 0.02% by weight or less. Moisture content is measured by loss on drying using Karl Fisher method. Hygroscopicity and moisture content are important characteristics of microspheres or beads as they may affect changes in active pharmaceutical ingredients (APIs), such as amorphous APIs, APIs sensitive to hydration, micronized and freeze-dried APIs that are sensitive to moisture.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a dissolution of less than about 2%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a dissolution of less than about 1.5%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a dissolution of less than about 1%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a dissolution of less than about 0.5%. Dissolution is determined as set forth herein in Example 2.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 5%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 4%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 3%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 2%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 1%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.9%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.8%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.7%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.6%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.5%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.4%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.3%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.2%. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an oil adsorption capacity of less than about 0.1%. Oil Adsorption Capacity is determined as set forth herein in Example 2.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an actual to predicted surface area ratio of about 5 or less. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an actual to predicted surface area ratio of about 4 or less. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an actual to predicted surface area ratio of about 3 or less. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have an actual to predicted surface area ratio of about 2 or less. The actual to predicted surface area ratio is calculated by dividing the actual surface area as determined by Nitrogen Gas Adsorption divided by the predicted surface area determined by laser particle size analysis. These methods and calculations are set forth herein in Example 2.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres are water soluble. Insoluble materials can interfere with the full delivery of an API. Insoluble materials can also become an issue in the formation of complete solutions, plugging needles and filters.
In some embodiments, the properties of material candidates for use in the present invention is a material that melts at less than 250° C. or a material that dissolves in, melts with or disperses in the primary material. In some embodiments, the material solidifies from a melt on cooling. In some embodiments, the material solidifies from a melt on cooling, in less than 10 minutes. In some embodiments, cooling can be achieved by chilled or non-chilled air, chilled gases or chilled liquids in which the material has limited solubility. In some embodiments, the solidification process forms very thin and parallel deposited crystalline plates that assimilate with each other into a tight crystal packing group. In some embodiments, the material that forms the microsphere is a substance with a trans chemical structure with a molecularly balanced charge distribution and thus a low dielectric constant (<10). In some embodiments, the material is made up of preferred similar sized and preferred uniformly distributed functional groups, like hydroxyl groups, that do not hinder greatly the bond formation process on cooling, and can form bonding structures rapidly through, as an example, hydrogen bonding almost instantly. In some embodiments, the microsphere material is based on fusion, co-crystallization and/or occlusion of the material into the crystal structure.
In some embodiments, microspheres of the present invention can be comprised of many different materials including, but not limited to, carbohydrates, polyols, sugars, starches, waxes, polyethylene glycol, cetyl alcohol, stearic acid, fatty acids, fatty acid esters, polyethylene glycol derivatives, materials miscible with these materials, or combinations thereof. In some embodiments, the microspheres do not include cellulose. In some embodiments, the microspheres do not include lipids. In some embodiments, microspheres of the present invention can be comprised of one or more materials that melt. In some embodiments, microspheres of the present invention can be comprised of a material that is solid at room temperature. In some embodiments, microspheres of the present invention can be comprised of one or materials that are crystalline solid at room temperature. In some embodiments, microspheres of the present invention can be comprised of one or more amorphous materials, such as melts.
In some embodiments, microspheres of the present invention can also contain additives including, but not limited to, maltodextrins, microcrystalline cellulose, hydroxypropyl methyl cellulose, methyl cellulose, polyvinyl alcohol, sodium CMC, povidone and other vinyl derivatives, calcium carbonate, tartaric acid, alginic acid, talc, titanium oxide, color, flavor, sodium lauryl sulfate, ph adjusters, surface active agents or combinations thereof.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres comprise a single core material. In another embodiment, composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is a polyol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is mannitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is 100% mannitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is sorbitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is maltitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is isomalt. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is erythritol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a core material, and wherein the core material is xylitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise one or more core materials. In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres have a crystalline structure.
In some embodiments of the present invention, a composition comprises a plurality of microspheres, wherein the microspheres comprise a single material. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise a polyol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise 100% mannitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise sorbitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise maltitol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise erythritol. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise isomalt. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise xylitol.
In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise one or more polyols. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and sorbitol. The ratio of mannitol to sorbitol can vary. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and sorbitol, wherein the mannitol:sorbitol ratio ranges from about 99.5:0.5 to about 90:10. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and xylitol. The ratio of mannitol to xylitol can vary. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and xylitol, wherein the mannitol:xyltiol ratio ranges from about 99.5:0.5 to about 90:10. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and erythritol. The ratio of mannitol to erythritol can vary. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and erythritol, wherein the mannitol:erythritol ratio ranges from about 99.5:0.5 to about 90:10. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and maltitol. The ratio of mannitol to maltitol can vary. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and maltitol, wherein the mannitol:maltitol ratio ranges from about 99.5:0.5 to about 90:10. In another embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and isomalt. The ratio of mannitol to isomalt can vary. In one embodiment, a composition comprises a plurality of microspheres, wherein the microspheres comprise mannitol and isomalt, wherein the mannitol:isomalt ratio ranges from about 99.5:0.5 to about 90:10. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of a polyol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of two or more polyols. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of mannitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of a mannitol and sorbitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of maltitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of erythritol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of xylitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of isomalt. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of mannitol and xylitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of mannitol and erythritol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of mannitol and maltitol. In some embodiments, a composition comprises a plurality of microspheres, wherein the microspheres consist of mannitol and isomalt.
In some embodiments, microspheres of the present invention can be manufactured by various methods. In one embodiment, microspheres can be produced by prilling (spray chilling) of a melt of core material. In this embodiment, a melted material is poured into a heated pressure vessel. The heated pressure vessel is pressurized and the plug valve at the bottom of the vessel is opened to send the melt thru the spray line to the nozzle. The spray line is heated if necessary. The nozzle can also be heated if needed. The prills from the nozzle are collected.
In another embodiment, microspheres of the present invention can be made by melting a material and dropping the melt onto a spinning spin disc for formation of microspheres. The material is melted. In some embodiments, the material can be melted via pan, oven or use of a powder extruder to melt system, such as is available from Randcastle extruders (RCP-1000) (Cedar Grove, N.J.) to decrease time of material at melt.
In this embodiment, powder is fed in and RPM of unit is set to control feed rate of melt. Residence time of melt can be <2 minutes in unit and flow rate is consistent.
In another embodiment, microspheres can be made by melting a material as a powder in the spin disk head assembly (See Gold Metal Cincinnati Ohio Tornado unit). Once melted, the liquid material is spun into a stream which, by centrifugal force, is spread into a thin film and exits the disk as a ligament that breaks into droplets or exits as droplets. In a preferred embodiment, a surface spinner style disk is preferred with a diameter of 4 inches or more and speed capabilities of from 200 RPM to 11,000 RPM. The wheel RPM controls film thickness and thus droplet/bead/microsphere size. The microspheres are allowed to fall in room temperature or chilled air to cool. In some embodiments, once cooled, any twinning or chill damaged beads if present, can be removed with a bead shape shorter.
In another embodiment, microspheres of the present invention can be made by using a sonic nozzle system supplied by Brace (Alzenan, Germany) called the Brace Spherisator M. The unit consists of an oven in which one or two liquid vessels are stored. The oven can be heated up to 200° C. Thus thermal conditions can be maintained on the liquid to 200° C. In some embodiments, the bottle(s) containing the melt can be pressurized. If the head space is pressurized, the liquid will flow to a nozzle which is mounted in a sonicator. Both the amplitude and frequency of the sonic energy can be adjusted. The motion as the liquid exits the nozzle causes the liquid stream to separate and form drops. In some embodiments, a strobe can be used to see the droplets form. Based on amplitude as a gross adjustment and frequency as the finer adjustment, the droplets release as individuals approximately the size the droplet would be as a cylinder and surface tension then coverts it to a sphere.
In some embodiments, mannitol alone in a single nozzle or an API dissolved in or dispersed in the mannitol melt made into microspheres using this approach produce microspheres either as a pure mannitol microsphere or as a mannitol and API dispersion. In some embodiments, phenytoin, carbamazepine and folic acid are examples of APIs that can be dissolved/dispersed in the melt of mannitol at 180° C. The liquid is delivered from a single sonic nozzle. The nozzle vibrates in an up and down amplitude at a frequency to produce an individual droplet at the tip. The droplet is allowed to cool as it freely falls to form a solidified microsphere. Cooling can occur at room temperature or in a chilled environment. In some embodiments, as a signal nozzle setup using a 200 μm nozzle at a flow rate of 35 g/min with the amplitude and frequency set based on a strobe light to maintain droplet formation separation. Pressure on the vessel is maintained to maintain flow rate.
In some embodiments, a liquid can also be injected in a center nozzle of a two concentric nozzle setup. In one embodiment, the 100 μm outer and the 100 μm inner nozzle in the Spherisator M. The material can be a melted API or a non-volatile liquid containing dissolved API or a nanosized API suspension or mannitol melt or mannitol melt API solution or dispersion. The material is delivered under pressure from a vessel in an oven to maintain its temperature needed to maintain the melt. A coating of a material melt alone or coating of an API dissolved or dispersed in the material melt can form the outer shell.
The microspheres of the present invention are useful in various applications. In one embodiment, the microspheres of the present invention are useful in the manufacture of sustained and modified release beads for dosing active pharmaceutical ingredients (APIs) as multi-particulate systems. In another embodiment, the microspheres of the present invention are useful are carriers for APIs for subsequent manufacture into tablets. In another embodiment, the microspheres of the present invention are useful as a free flowing excipient in the manufacture of mini-tablets. In another embodiment, the microspheres of the present invention are useful in the manufacture of API dispersions.
In some embodiments, microspheres of the present invention are useful as core beads onto which an API is layered either in a suspension or a solution or dry powder alternated with a solution to create a tacky surface and if needed a functional coating also applied. In some embodiments, microspheres of the present invention are useful as core beads for immediate, modified and/or sustain release active and coated beads for inclusion into sachets, capsules and tablet formulations. In some embodiments, microspheres of the present invention are useful as placebo beads. In some embodiments, microspheres of the present invention are useful as cores for plating of APIs onto by lyophilization process.
In some embodiments of the present invention, microspheres can have a small particle size. In some embodiments, such microspheres of the present invention are useful for sachets and chewable tablets to reduce damage [to the API] during chewing, and to improve the mouthfeel of the tablet. In some embodiments, such microspheres are useful in all dosage forms to reduce final bead size yet allow for high API dose loading. In some embodiments, such microspheres are useful as they may allow for a greater thickness of API coating and thus a wider range of release rate options for use of thicker coatings giving a slower release. In some embodiments, such small microspheres are useful as they may reduce localized concentration of irritative drug by providing greater surface area. In some embodiments, small microspheres are useful as they may reduce variation in gastric emptying rate and transit time. In some embodiments, small microspheres are useful as they are less susceptible to dose dumping. In some embodiments, small microspheres are useful as they disperse more freely in gastrointestinal tract and invariably maximize API absorption and also reduce peak plasma fluctuation. In some embodiments, small microspheres are useful as they can be used as a free flowing excipient in mini-tablets.
In some embodiments of the present invention, pharmaceutical compositions comprise microspheres of the present invention and an active pharmaceutical ingredient (API). In some embodiments, pharmaceutical compositions comprise a plurality of microspheres and API. APIs useful in the present invention may include but are not limited to those described in the Physician's Desk Reference, 61st ed. Montvale, N.J.: Thomson PDR; 2007, which is incorporated by reference herein in its entirety. In some embodiments, the API may be present inside the microsphere. In another embodiment, the API may be present on the outside of the microsphere. In some embodiments, the microspheres of the present invention are useful in combination with standard methods of API incorporation into or onto beads or microspheres.
In some embodiments of the present invention, a blend of powder of a core material and an API can be added to a melt extruder and once melted would disperse in melt and discharge as a melt stream either to be pressure atomized or to a spin disc for creation of microspheres. In another embodiment, an API can be dissolved in a melted core material. In another embodiment, a second feeder position can be added to a two-stage melt extruder wherein the melt flowing at a controlled rate based on RPM of the melt extruder push melt past a powder entry point where a mixture of the API and as an option additional core material or other additives are delivered. The melt and API or API dispersion is then transferred though a mixing section of the extruder and then delivered to either the spin disc or to a pressurized atomizer unit.
In some embodiments, microspheres can be coated using a conventional pan system. The standard coating pan system consists of a circular metal pan mounted sat an angle on a stand, the pan is rotated on its horizontal axis by a motor, the hot air is directed into the pan and onto the bed surface, and is exhausted by means of ducts positioned through the front of the pan. Coating solutions are applied by spraying the material on the bed surface. As coating is applied the coating solution is dried off. It is common to dust powder mixtures onto wetted beads and dry the beads in layers. A final color and seal coating is often applied.
In some embodiments, microspheres can be coated using a perforated coating pan. Coating pan has perforations along its cylindrical portion. It is driven by a variable speed drive. Supply of hot air and exhaust of drying air are arranged to facilitate the coating system through stainless steel plenums positioned on both sides of the perforated coating pan. The pan is enclosed in an airtight housing provided with a suitable door and front glass window. This housing of pan with drive is a stainless steel cabinet accommodating the gearbox, AC variable drive, power panel, hot air unit, exhaust unit and an air fitter.
Liquid spray system is complete with stainless steel liquid storage vessel, variable flow-rate liquid dosing pump, automatic spray gun, and inter-connecting flexible hoses.
In some embodiments, the Fluid Bed Technology can be used to coat microspheres. The Fluid Bed Technology is the more modern approach to coating beads. It is a very efficient coating technique. The major advantage of the Fluid Bed Systems it is a closed system that air suspends the beads.
In a fluidized bed a coat is introduced to cover the core particles inside the bed. In the process, a layer is deposited onto the surface of fluidized solid particles by spraying with a solution of the coating material. The fluidizing gas is also use to dry the deposited solution. There is considerable diversity in methods of using fluidized bed technology. For e.g. liquids can be applied to fluidized particles in a variety of ways, including top, bottom and tangential spraying. For a given product, each method can offer markedly different finished product characteristics.
Fluidized beds are used for coating because of their high energy and mass transfer. Fluidized beds for film coating can be divided into three groups: top spray, tangential spray, and bottom-spray equipment.
In the top spray bed, the expansion chamber is lengthened to allow powder to remain fluidized longer and to move with a higher velocity, so that agglomeration is minimized. The expansion chamber is conically shaped to allow uniform deceleration of air stream. The filter housing is larger and designed to shake the fines back into the bed interrupting fluidization; this reduces agglomeration tendencies. The nozzle is positioned low in the expansion chamber so that coating material impinge on the fluidized particle a short distance from the nozzle; this reduces droplet spray drying and provides for longer subsequent drying of the coated particles. The top spray coater has been used to apply aqueous and organic solvent based film coatings, controlled release coatings. Smaller microspheres in this technique would allow smaller final beads and/or thicker coatings.
In the bottom spray coating, the Wurster machine employs a cylindrical product container with a perforated plate. Inside the container is a second cylinder (coating partition) with is raised slightly above the perforated plate, centered in the plate below this partition is a spray nozzle used to dispense the coating solution. The perforated plated is designed with large holes in the area under the coating partition and smaller holes in the remainder of the plate, except for one ring of large holes at the perimeter. The design allows the core particles to be pneumatically transported upward through the coating partition, and downward outside this partition. Material passing through coating partition receives a layer of coating material, dries in the expansion chamber, and falls back in a semi fluidized state. Material circulates rapidly in this fashion and receives layer of coating material, dries in the expansion chamber, and falls back in a semi fluidized state material circulates rapidly in this fashion and receives a layer of coating on each pass through the coating partition. The ring of large holes on the periphery of perforated plate prevents the accumulation of material at the container wall. It is used for coating small particles, beads, tablets and capsules.
The tangential spraying system, which is commonly fitted with a rotating bottom plate, can achieve film quantities nearly as good as the bottom spraying system. The rotation of the plate nicely supports product movement, so that the required air amount is mainly used for drying process and only to a smaller degree for the product movement.
In some embodiments, microspheres can be coated using a fluid bed coating system. In some embodiments, for microspheres of small sizes the coating film is used to control the release rate of the API. Microspheres allow the loading of the API in a coating layer first up to 1 to 2 mm in diameter followed by the application of the release controlling layer. Thus the small particle diameter of the microsphere add to API loading capacity and the decreasing particle diameter and thus the specific surface area of a substrate increase dramatically and the required coating weight gain is not experienced. Drug layering can be applied more rapidly than the final controlled coating layer.
In some embodiments, microspheres can be coated using granulation. Granulation techniques utilizing centrifugal fluidizing disk that can be moved up or down to create a variable slit opening between the outer perimeter of the disk and the sidewall of the container. Air is drawn into the product container through the slit under negative pressure. This fluidizes the material along the circumferential surface of the product container. At the same time the disk rotates at varying speeds and moves the product by the centrifugal force to the outer portions where it is lifted by the fluidizing air stream into the expansion chamber. As the material decelerates, it descends to the center of the disk and repeats the same sequence.
The fluidization pattern is often described as a spiraling helix or rope-like pattern around the inside of the rotor chamber.
Spray nozzles can be immersed in the bed of fluidized material and spray applied in tangential fashion with respect to the particle flow. Microspheres in this process allows for a starting controlled surface onto which the coating powder with API can be layered onto and held by a spray solution of coating materials in a rapid layering application approach. Based on the uniformity of both shape and size these microspheres allow for a uniform and rapid gain in weight and maintain separation readily versus standard crystal seeds currently used and starter material.
The various embodiments of the composition, according to the present invention, may be used in a variety of dosage forms including, but not limited to, chewable tablets, swallow tablets, soft chews including tablets and soft gel capsules, orally disintegrating tablets, orally dispersible powders, mini-tablets, lozenges, film strips, gums, gels, ointments and creams, tablet inserts (eye, ear, vaginal), suppositories, hard shell capsules, liquid fill capsules, liquid suspensions and sustained release beads.
In some embodiments, the dosage form may include a pharmaceutically acceptable ingredient including excipients, orally disintegrating excipients, including, but not limited to Pharmaburst 500 (SPI Pharma, Inc.); diluents; disintegrants; binders; fillers; bulking agent; organic acid(s); colorants; stabilizers; preservatives; lubricants; glidants/anti-adherants; chelating agents; vehicles; bulking agents; stabilizers; preservatives; tonicity adjusting agents; local anesthetics; pH adjusting agents; antioxidants; osmotic agents; chelating agents; viscosifying agents; wetting agents; emulsifying agents; acids; sugar alcohol; reducing sugars; non-reducing sugars and the like used either alone or in combination thereof. In some embodiments, the pharmaceutically acceptable ingredients may include excipients, binders, lubricants, sugar alcohols, disintegrating agents, colors, flavors and the like used either alone or combinations thereof.
In some embodiments, a pharmaceutical formulation comprising a plurality of microspheres may be used in a directly compressible dosage form. The term “directly compressible” means that the composition can be compressed to tablet form on standard tableting machines (including, but not limited to high speed tableting machines) using standard (i.e., without any specially machined, shaped or coated surfaces) punches and dies, without any significant amount of the composition adhering to the punches and dies by applying compressive pressure to the composition. In some embodiments, the compression pressure ranges from 60 MPa to 170 MP. In some embodiments, the compression force ranges from 80 MPa to 150 MPa. In some embodiments, the compression pressure is at least 60 MPa.
The term “pharmaceutical formulation” as used herein refers to formulations containing the composition of the present invention in combination with carriers or excipients suited to a selected drug delivery platform, e.g., a capsule, an orally dispersible formulation, an effervescent formulation, a chewable tablet, a lozenge, a hard or swallow tablet, or the like.
“Carriers” or “vehicles” as used herein refer to carrier materials suitable for oral drug administration, and include any such materials known in the art, e.g., diluents, binders, granulating agents, disintegrants, lubricating agents, colorants, flavoring agents, and the like.
In some embodiments, various types of pharmaceutical formulations may be prepared using the presently disclosed microspheres and compositions, including powders, chewable tablets, orally dissolving tablets, effervescent formulations, and liquid dispersions. For solid formulations such as powders, chewable tablets, orally dissolving tablets and effervescent formulations, conventional carriers, excipients and additives can be employed, including diluents, binders, granulating agents, disintegrants, flavoring additives, and the like. Examples of the normally employed excipients include pharmaceutical grades of mannitol, lactose, starch, and the like. Liquid pharmaceutical compositions containing the present microspheres will generally be prepared by dispersing or suspending the microcapsules in a non-aqueous carrier which does not cause release of the drug, or else by dispersing the microspheres or composition in an aqueous carrier immediately prior to administration to the patient. In some embodiments, the microspheres or composition may be provided as a free-flowing particulate material, as in a sachet or other suitable package, and such a particulate material may be dispersed in an aqueous carrier. These solid or liquid formulations may contain any amount of the microsphere or composition needed to provide the desired amount of the active ingredient contained in the microsphere or composition. In some embodiments, amounts of microspheres or composition on the order of about 10 wt. % to about 95 wt. % of the dosage form may be used. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art.
It will be apparent to one of skill in the art that various modifications and variations can be made in the methods and compositions of the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention cover the modifications and variations of the present invention provided they come within the scope of the appended claims and their equivalents.
The following examples are given to illustrate the present invention. It should be understood, however, that the invention is not to be limited to the specific conditions or details described in these examples. Throughout the specification, any and all referenced publicly available documents, including but not limited to a U.S. patent, are specifically incorporated by reference.
Mannitol (EP) (Shandong Tianli Pharmaceutical Ltd., Guangzhou, China) and mannitol/sorbitol (2.3%) (sorbitol, Roquette, Keokuk, Iowa) microspheres were produced by prilling (spray chilling) of a melt of the polyols in a stainless steel 2 quart sauce pan. The melted polyol was poured into a heated pressure vessel that was heated by electrical heat bands. The heated pressure vessel was pressurized to 50 psig and the plug valve at the bottom of the vessel was opened to send the mannitol thru the spray line to the nozzle. The spray line was heated by electrical heat tape. The nozzle was heated with a propane torch prior to opening the valve. The prills from the nozzle (Spraying Systems 6501) were collected on plastic and bagged for evaluation. Microspheres particle size distribution was determined by Malvern Mastersizer Laser analysis (Malvern, Pa.). Table 1 shows the particle size distribution of the microspheres. Microsphere diameters were in the 250 μm mean range with a broad distribution (d(0.1)=124 and d(0.9)=473 um or 473/124=3.8 to 1 distribution ratio and d(0.1)=204 and d(0.9)=598 um or 598/204=2.9 to 1 distribution ratio.
Mannitol EP (Shandong Tianli Pharmaceutical Ltd., Guangzhou, China), mannitol/sorbitol (2.3%) (sorbitol, Roquette, Keokuk, Iowa) and mannitol/polysorbate 80 (Unichema, New Castle, Del.) microspheres were made by melting polyol and dropping melt to spin disc for formation of microspheres. Mannitol is melted in pan or oven at 10° C. above its melting point for mannitol a temperature higher than 176° C. Once melted, the liquid mannitol is spun into a stream which, by centrifugal force, is spread into a thin film and exits the disk as a ligament that breaks into droplets or exits as droplets. Surface spinner style disk is preferred with a diameter of 4 inches or more and speed capabilities of from 500 RPM to 11,000 RPM. Wheel RPM controls film thickness and thus droplet/bead size. Microspheres are allowed to fall at least 8 feet in room temperature or chilled air to congeal. Once congealed a coarse screen can be used to complete cooling and maintain separation. Any twinning or chill damaged microspheres are removed with a bead shape shorter.
Microspheres were analyzed for particle size using Malvern Mastersizer. Table 2 shows the particle size distribution (PSD) of the microspheres.
Scanning electron microscopy (SEM) was performed on exemplary mannitol microspheres.
Approximately 1.0 g of the mannitol EP microspheres, Colorcon SureSpheres® sugar/starch spheres (Colorcon, West Point, Pa.), and Werner Pharm-a-Sphere™ Neutral Pellets (Hanns G. Werner GmbH, Tornesch, Germany) were analyzed for moisture content using in the Karl Fisher method described in US Pharmacopeia (USP) 26. Table 3 shows the moisture content of the mannitol microspheres, SureSpheres, and Pharm-a-Spheres.
0.2 g of the mannitol EP microsphere was analyzed on an AquaDyne Instrument of the Quantachrome (Quantachrome Instruments Palm Beach Fla.) using the aquaWin—Data Acquisition and Reduction to measure dynamic vapor sorption.
In order to compare the hygroscopicity of the mannitol microspheres to other forms of mannitol, powdered (Mannogem® powder, Lot #121101399F, SPI Pharma, Inc.; Pearlitol 50C, Lot # KVKRN, Roquette Freres), granular (Mannogem granular, Lot #121101116G, SPI Pharma, Inc.; Pearlitol 400DC, Lot # E592J, Roquette Freres), and sprayed-dried mannitol (Mannogem EZ, Lot #121101324, SPI Pharma, Inc.; Pearlitol 200 SD, Lot #E983G, Roquette Freres) products were submitted to Quantachrome for Dynamic Vapor Sorption (DVS) analysis.
The adsorption profiles for Mannogem powder and Pearlitol 50c are compared in
The adsorption profiles for Mannogem granular and Pearlitol 400DC are compared in
The adsorption profiles for Mannogem spray dried and Pearlitol 200SD are compared in
None of the above adsorption profiles for the powder, granular and spray-dried mannitol-based commercial products are below 0.2% moisture content at 95% RH. As seen in
Mannitol microspheres was measured by differential scanning calorimetry (DSC) using a Thermal Analysis (New Castel, Del.) DSC Q2000 instrument with a version V23.5 data acquisition system at 10° C. per minute from room temperature to 300° C.
Mannitol EP microspheres and Nonpareil-108 beads (Freund Industrial Co., Japan) were analyzed for skeletal density using an Ultrapyc 1200e V4.02 of Quantachrome Corporation (Palm Beach, Fla.). Table 4 shows the skeletal density of mannitol microspheres. Table 5 shows the skeletal density of Nonpareil-108 beads.
The closeness of the helium pycnometry skeletal density to the true density of alpha mannitol at 1.468 gm/cm−3 (Burger et al. *) versus 1.4590 to 1.4651 for samples indicates microspheres are solid structures and substantially lack interior voids/porosity. Whereas the skeletal density of NP-108 is 1.4552, which indicates that it is not a pure polymorphic structure.
Scanning electron microscopy (SEM) was performed on sectioned mannitol microspheres to examine the interior structure of the microspheres.
SEM was also performed on current commercial microspheres/beads: Celphere CP-102 microcrystalline cellulose beads (Asahi Kasei Corporation, Tokyo, Japan), MCell 400 mannitol beads (Pharmatrans Sanaq AG, Allschwil, Switzerland), Pharm-a-Sphere™ Neutral Pellets (Hanns G. Werner GmbH, Tornesch, Germany), SureSpheres® sugar/starch spheres (Colorcon, West Point, Pa.), Nonpareil-108 (NP-108) mannitol beads (Freund Industrial Co., Japan).
Characterization of the sphericity or circularity of microspheres was conducted by Particle Technology Labs (Downers Grove, Ill., USA). Automated microscopy and image analysis techniques (Malvern Morphologi G3S automated particle image analysis system, Malvern Instruments Inc., USA) were used to characterize the morphology of microspheres of the present invention, and to compare to current commercially available microspheres, and to calculate mean circularity, aspect ratio, convexity and solidity of each.
The circularity of each of the various microspheres was determined. Circularity is a measurement of the calculated peripheral length of a circle of the same silhouetted area of a particle's blocking a light source/the particle's actual peripheral length with values in the range from 0-1. A perfect circle has circularity of roundness 1.0, while a needle-shaped object has roundness close to 0. Table 6 shows the circularity of various shapes (Image Analysis: Evaluating Particle Shape by Horiba Particles on Jul. 7, 2011 by Jeff Bodycomb www.horiba.com).
The circularity—is typically determined using the equation:
Circularity=2(π area)̂0.5/P
where A is the measured area and P is the perimeter length of the microspheres. Circularity is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
In this depiction, the 0.886 circularity relates to the shape of a square or in three dimensions a cube and indicates the particle has sharper point or corners in its surface structure and would tumble verses roll. Tumbling is damaging to coating and causes segregation on shape and sharp edges cause issues in both coating stress and coating distribution, resulting in uneven coating thickness and coat cracking Sharp edges on the bead surface can also break off and become incorporated in the coating and cause cracking and early release issues. Sharp edges can also add stress to the coating as it dries and cause the coating to crack. The presence of cracking can lead to the need to use more plasticizer which causes the coated particle to be tackier. Table 7 shows the circularity of the various microspheres tested.
The aspect ratio of each of the microspheres was also determined. Aspect ratio is defined as the ratio of the length of a sphere divided by the width, with the microspheres being considered circular (spherical) if the aspect ratio lies between 0.95 and 1.00. Table 8 shows the aspect ratios of various shapes (Image Analysis: Evaluating Particle Shape by Horiba Particles on Jul. 7, 2011 by Jeff Bodycomb www.horiba.com). The aspect ratio is sensitive to how isometric the particle is. Particles with a high aspect ratio not only tumble but they tend to lodge in pore spaces in the coating bed and bounce as they tumble. This is a measure of rod, plate or needle-like characteristics of a particle.
Aspect ratio is the ratio of the shortest diameter of particle to the longest diameter of a particle. Aspect ratio is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008). Feret measured diameters as parallel lines brought in to touch particle at any angle. Thus it is the shortest separation of these lines divide by the longest separation.
Aspect Ratio is peak height but not crevice biased. The closer the aspect ratio is to 1 the more free rolling, less tumbling, mechanically interlocking and bouncing is a particle will it flows during the process of coating.
Table 9 and Table 10 show the aspect ratios of the microspheres tested and
The microspheres of the present invention and current commercial microspheres/beads were examined for solidity. Solidity looks for missing areas caused by risers or indentations in the surface of the microsphere or particle. In order to determine the solidity, a cord is wrapped around the microsphere to approximate the area of the microsphere or particle without convex (indented areas) due to crevices and risers off the surface. The area of the microsphere or particle is exactly measured as the shadow of the image of the microsphere or particle in a light path. The area of the microsphere or particle is then divided by the area inside the cord stretched over the microspheres' or particles' outer surface. Solidity is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008).
A sphere has a solidity of 1. A cube, a triangle (pyramid) or a rod would also have a solidity of 1. Although a cube and a pyramid have corners/edges, they do not have surface risers or crevices. Any surface indentations or surface bumps would add to the area inside the cord. Thus solidity as a factor is then related to the area associated with the convexity area of the microsphere as area lost ratio to solidity. A surface without convexness can be directly coated in layers. Each layer at the start is the base layer and grows uniformly in thickness. Issues with convexed areas is removing the air, getting the film in the tighter space uniformly and building the layer in the space to the surface to allow for a uniform outer layer coating thickness. Extra time is spent, smaller droplets sizes of coating spray may be required, more plasticizer needed to allow the film to bridge without cracking if the crevice isn't filled and extra coating material is used.
In three dimensions it is also related to the extra volume associated with this convexed space. Which either coating fills or bridges over creating coating stress, imperfections and variation in coating amount needed to obtain a functional coating. Table 11 shows the solidity of the microspheres tested and
Convexity
The microspheres of the present invention and current commercial microspheres/beads were also examined for convexity. Convexity is similar to solidity but focuses more on surface smoothness. Here the most accurate measurement is the periphery of the particle. What the approximation is in this index is the cord length that is drawn surrounding the particle which is the same cord length as in the solidity measurement. If the surface is perfectly smooth and without crevices or risers the convexity will be equal to one. Convexity is calculated in accordance with International Organization for Standardization (ISO) 9276-6 (2008). Table 12 shows the convexity of the microspheres tested and
The microspheres of the present invention and current commercial microspheres/beads were also examined for determination of surface area and porosity. Surface area measurements of the samples were performed using a Tristar II 3020 surface area analyzer made by Micromeritics (Malvern, Pa.). The analysis gas was nitrogen; the analysis temperature was 77.4 K, cold free space was 40.1922 to 41.4298 cm3 measured and the warm free space was 13.1291 to 13.4346 cm3 measured. The equilibration interval was 20 seconds, and the sample density was assumed to be 1.000 gm/cm3. The mass of the samples was accurately weighed to approximately 2.5 gms. Single point BET was run at a relative pressure of 0.15 to 0.20 and extrapolated to zero. Multipoint was done at least two additional relative pressure points below the single point measurement relative pressure. Tristar II 3020 V1.04 software was used to calculate the single point and multipoint BJH adsorbed and desorbed surface area.
The pore volume measurements were performed on the same instrument used for surface area measurement at relative pressure up to 1.0. A sample accurately weight of approximately 0.5 gms was used. Ten or more relative pressure from 0.01 to 1.0 was used. Tristar II 3020 V1.04 software was used to calculate the pore volume. Table 13 shows the surface area and porosity of the various microspheres examined. Data comparing surface area and pore volume clearly shows the Mannosphere microspheres of the present invention have less surface area than the Sure-Sphere and Pharm-a-Sphere beads in single point and in both the BJH multipoint adsorption and desorption methods. This lack of pore volume and lower surface area per gram is due to Mannosphere microspheres' shape being more spherical, and the lack of internal surface compared to the Sure-Sphere or Pharm-a-Sphere beads or is due to bead size.
Particle size for each of the microsphere samples was measured by laser particle size analysis using a Microtrac 5350 made by Microtrac Corporation (Montgomeryville, Pa.). Approximately a one gram sample was used for the test using the Turbotrac dry feeder at an energy setting of 10 and a range of 0.687 to 995.6 μm and a 10 second run time. The samples particle size and the samples projected surface area as a perfect was calculated by the Microtrac Flex version 10.4 program supplied with the analyzer. Data on particle size is listed in the Table 14. All samples were assigned a 1.0 gm/ml density to convert volume values to grams.
Table 15 compares the size estimate of surface area in the multiparticulate sample to the actual surface area measured by nitrogen gas adsorption. Mannosphere microspheres show a much closer prediction of size of beads to surface area of the bead, which is only going to be typical of true spherical beads lacking internal porosity. The Mannosphere microspheres are smaller in particle size (259 μm) to Sure-Spheres beads (413 μm). Thus from microsphere size data, Sure-Sphere beads are expected to have a lower surface area. The actual surface area in Sure-Sphere beads is (0.26/0.04)=6.5 times more than Mannosphere microspheres. Similarly the Pharm-a-Sphere beads are smaller (175 μm), yet they have 5.5 times more surface area than predicted. The actual to predicted surface area shows how much closer Mannosphere microspheres have a size predicted surface area for coating.
This data along with the pore volume measurements shows the substantial lack of internal surface as well as the closer match to prefect sphericity by the microspheres of this invention compared to standard mass marketed microspheres or beads. This tighter microsphere size to surface control allows for a much more accurate use of size to control both amount of surface coating per batch but control the surface location as more on the outer surface of the bead versus interior to the microsphere.
The microspheres of the present invention and current commercial microspheres/beads were also examined for determination of dissolution. The dissolution of microsphere samples was performed in 20 mL glass vial with cap. (Fisher Scientific, Hampton, N.H.) 10 mL of deionized water was added to an accurate weight of approximately 500 mg of microspheres. The vials were place into the holder of the Rotatest Ping-Pong model 51500-10 reciprocating shaker (Cole Parmer, Vernon Hills, Ill.) and its platform rotated at 300 rpm. After shaking for test time, the samples were filtered through pre-weighed 0.45 micron filter paper (Nylon Membrane filters, 0.45 micron, 47 mm dia, Cat #7404004, Whatman (Fisher Scientific, Hampton, N.H.)). The filter paper was dried in a conventional oven at 60° C. and then placed in a suitably sized glass desiccator (Fisher Scientific, Hampton, N.H.) using fresh drierite (Sigma Aldridge, St. Louis, Mo.). Once cooled the filter paper was re-weighed to calculate the weight change in mg. Table 16 shows the dissolution of various microspheres. The data shows the limited amount of <0.5% undissolved at both 5 and 10 minutes of testing compared to NP-108 microspheres at 2.0% in 5 minutes and 0.92% in 15 minutes, and Sure-Sphere and Pharm-a-Sphere beads with >10% undissolved even at 15 minutes of shaking 100% dissolution or solubility is often desirable in situations in which very low dose actives are used in coatings to obtain complete recovery of actives.
The microspheres of the present invention and current commercial microspheres/beads were also examined for oil adsorption. 1.00 g of each microsphere sample was accurately weighed. The sample was added to a mortar and pestle and 0.02 g of Light Mineral Oil, USP/NF (Sigma Aldrige, St. Louis, Mo.) was added drop-wise (Transfer pipet, disposable, standard, polyethylene, one-piece, single squeeze draws up to 3.2 mL, length 5.875 in., capacity 7.7 mL; Fisher Scientific, Hampton, N.H.). The oil was thoroughly incorporated into the sample by lightly mixing, but not crushing in the mortar and pestle (Mortar and Pestle: Coors, USA. Cat #60319, Fisher Scientific, Hampton, N.H.) for 3 minutes. The samples were visually observed to determine is the sample was free flowing or sticky/agglomerated. Table 17 shows the oil adsorption for the various microspheres. None of the samples tested was able to adsorb 2% oil and remain as free flowing beads. All of these beads are constructed to have minimum oil adsorption capacity.
Erythritol EP (Baolingbao Biology Co., LTD., China) was added to a Tornado Spin Disc (Gold Metal Cincinnati Ohio). The units spin head was heated to ˜160° C. while spinning at 3400 RPM and made microspheres with a PSD of d(0.1)=131 μm, a d(0.5)=262 μm and a d(0.9)=371 μm. The size distribution ratio is 2.8 to 1 for this run. The DSC of these beads shows a single and sharp melt peak at 121.6° C. with a heat of fusion of 273.1 J/g. A pure crystalline erythritol melt range of 199° C. to 121° C. is expected and thus crystal structure of formed microsphere is a standard and highest energy erythritol polymorph.
This application claims priority to U.S. Provisional Application No. 61/638,073 filed Apr. 25, 2012 and U.S. Provisional Application No. 61/783,603 filed Mar. 14, 2013, which are hereby incorporated by reference in their entireties.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US13/38257 | 4/25/2013 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 61783603 | Mar 2013 | US | |
| 61638073 | Apr 2012 | US |
