Claims
- 1. A method for producing coagulation Factor VIII which comprises culturing a Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant and a cyclodextrin and collecting the secreted coagulation Factor VIII from the culture medium, wherein the nonionic surfactant is selected from the group consisting of a pluronic surfactant and a sorbitan surfactant.
- 2. The method of claim 1 wherein the surfactant is PLURONIC F-68.
- 3. The method of claim 1 wherein the nonionic surfactant and cyclodextrin are each present at a concentration of from 0.005 to 1.0% and 0,001 to 0.3%, respectively.
- 4. A method for producing coagulation Factor VIII which comprises culturing a Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant selected from the group consisting of a PLURONIC surfactant and a sorbitan surfactant, and a cyclodextrin and collecting the secreted coagulation Factor VIII from the culture medium, wherein the cyclodextrin is selected from the group consisting of unmodified .alpha.-cyclodextrin and methylated .alpha.-cyclodextrin.
- 5. A method for producing coagulation Factor VIII which comprises culturing transformed Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant selected from the group consisting of a PLVRONIC surfactant and a sorbitan surfactant, butyric acid or a salt thereof in a concentration of from 0.1 to 5.0 mM, a lithium salt in a concentration of from 2.0 to 50 mM, and a cyclodextrin, and collecting the secreted coagulation Factor VIII from the culture medium.
- 6. A culture medium, optionally containing a low level of protein, for culturing a transformed Chinese Hamster Ovary Cell which produces coagulation Factor VIII, which comprises a nonionic surfactant selected from the group consisting of a PLURONIC surfactant and a sorbitan surfactant and a cyclodextrin in admixture with a conventional culture medium.
- 7. The culture medium of claim. 6 wherein the nonionic surfactant is a PLURONIC surfactant selected from the group consisting of PLURONIC F-61, PLURONIC F-68, PLURONIC F-71 and PLURONIC F-108 or a sorbitan surfactant selected from the group consisting of polyoxyethylene sorbitan monolaurate and polyoxyethylene sorbitan monooleate.
- 8. The culture medium of claim 6 wherein the cyclodextrin is a member selected from the group consisting of unmodified .alpha.-cyclodextrin and methylated .alpha.-cyclodextrin.
- 9. The culture medium of claim 6 wherein the PLURONIC surfactant and the cyclodextrin are each contained at a concentration of 0.005 to 1.0%, and 0.001 to 0.3%, respectively.
- 10. The culture medium of claim 6 wherein the sorbitan surfactant and the cyclodextrin are each contained at a concentration of 0.001 to 0.006%, and 0.001 to 0.3%, respectively.
- 11. The culture medium of any of claims 6 to 10 which additionally contains butyric acid or a salt thereof and a lithium salt at a concentration of 0.1 to 5.0 mM and 2.0 to 50 mM, respectively.
Priority Claims (2)
Number |
Date |
Country |
Kind |
2-121729 |
May 1990 |
JPX |
|
3-082269 |
Apr 1991 |
JPX |
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Parent Case Info
This application is a continuation of U.S. application Ser. No. 07/696,567 filed May 8, 1991, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4216203 |
Johnston |
Aug 1980 |
|
4533637 |
Yamane et al. |
Aug 1985 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
57-74093 |
May 1982 |
JPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
696567 |
May 1991 |
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