Claims
- 1. A purified and isolated polynucleotide encoding cGB-PDE.
- 2. The polynucleotide of claim 1 which is a DNA sequence.
- 3. The DNA sequence of claim 2 which is a cDNA sequence or a biological replica thereof.
- 4. The DNA sequence of claim 2 which is a genomic DNA sequence or a biological replica thereof.
- 5. An RNA transcript of the genomic DNA sequence of claim 4.
- 6. The DNA sequence of claim 2 which is a wholly or partially chemically synthesized DNA sequence or a biological replica thereof.
- 7. The DNA sequence of claim 4 further comprising an endogenous expression control DNA sequence.
- 8. A DNA vector comprising a DNA sequence according to claim 2.
- 9. The vector of claim 8 wherein said DNA sequence is operatively linked to an expression control DNA sequence.
- 10. A host cell stably transformed or transfected with a DNA sequence according to claim 7 in a manner allowing the expression in said host cell of cGB-PDE polypeptide possessing a ligand/receptor binding biological activity or immunological property specific to cGB-PDE.
- 11. A method for producing cGB-PDE polypeptide, said method comprising growing a host cell according to claim 10 in a suitable nutrient medium and isolating cGB-PDE polypeptide from said cell or the medium of its growth.
- 12. A polypeptide or peptide capable of specifically binding to cGB-PDE.
- 13. An antibody substance according to claim 12.
- 14. A monoclonal antibody according to claim 13.
- 15. A hybridoma cell line producing a monoclonal antibody according to claim 14.
- 16. A humanized antibody substance according to claim 13.
- 17. An antisense polynucleotide specific for a polynucleotide encoding cGB-PDE.
- 18. A DNA sequence encoding cGB-PDE and selected from the group consisting of:
(a) the DNA sequence set out in SEQ ID NO: 9 or 22; (b) a DNA which hybridizes under stringent conditions to the DNA of (a); and (c) a DNA sequence which, but for the redundancy of the genetic code, would hybridize under stringent conditions to a DNA sequence of (a) or (b).
Parent Case Info
[0001] This application is a continuation-in-part of co-pending U.S. patent application Ser. No. 08/068,051 filed May 27, 1993.
Government Interests
[0002] Experimental work described herein was supported in part by Research Grants GM15731, DK21723, DK40029 and GM41269 and the Medical Scientist Training Program Grant GM07347 awarded by the National Institutes of Health. The United States government has certain rights in the invention.
Divisions (1)
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08463949 |
Jun 1995 |
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Child |
09055584 |
Apr 1998 |
US |
Continuations (2)
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09599658 |
Jun 2000 |
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10115515 |
Apr 2002 |
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09055584 |
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09599658 |
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Continuation in Parts (1)
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08068051 |
May 1993 |
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08463949 |
Jun 1995 |
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