Patent Application
20080227820
The present invention relates to compounds of formula I, a process for their production and their use as anti-inflammatory agents.
From the prior art of DE 100 38 639 and WO 02/10143, anti-inflammatory agents of the following general formula
are known, in which the Ar radical comprises phthalides, thiophthalides, benzoxazinones or phthalazinones. In the experiment, these compounds show dissociations of action between anti-inflammatory and undesirable metabolic actions and are superior to the previously described nonsteroidal glucocorticoids or exhibit at least just as good an action.
Further compounds are disclosed in WO 2005/035518.
However, the selectivity of the compounds of the prior art towards the glucocorticoid receptor (GR) compared to the other steroid receptors still requires improvement.
It was therefore the object of this invention to make compounds available whose selectivity towards the glucocorticoid receptor (GR) is improved compared to the other steroid receptors.
This object has been achieved by the compounds according to the claims.
This invention therefore relates to stereoisomers of general formula I
in which
The indole or indazole group in formula I can be bound by the 4-, 5-, 6- or 7-position to the amino group of the tetrahydronaphtalene group. Preferably, the indole or indazole group is bound by the 5- or 4-position. Most preferred is the 4-position.
If R5 is a pyrimidinyl or a pyridinyl group this group may be bound by any position to the indole or indazole ring.
Unless otherwise notified the term “alkyl” refers to straight or branched derivatives. For example, the term propyl comprises n-propyl and iso-propyl, the term butyl comprises n-butyl, iso-butyl and tert-butyl.
Unless otherwise notified the term “haloalkyl” refers to straight or branched alkyl derivatives in which at least one hydrogen is substituted by a halogen atom, preferably by a fluoro atom. For example, the term halopropyl comprises perfluoro-n-propyl and perfluoroiso-propyl, as well as 1-chloro propyl, the term haloethyl comprises 1-fluoroethyl, 2,2,2-trifluoroethyl, 1-chloroethyl and 2-chloroethyl.
Unless otherwise notified the term “alkoxy” refers to straight or branched derivatives. For example, the term propoxy comprises n-propoxy and iso-propoxy, the term butoxy comprises n-butoxy, iso-butoxy and tert-butoxy.
Compounds of general formula I, in which in which at least one of the groups R1-R3 is selected from the group consisting of Fluoro, Chloro, Hydroxy, C1-C3-Alkyl, C1-C3-Alkoxy, or in which two of the groups R1-R3 together form a group —O—CH2—O— or —CH2—CH2—O— are a preferred subject of the invention. Preferred groups R4 are Hydrogen, Fluoro, Chloro, C1-C3-Alkyl, C1-C3-Alkoxy.
Preferred groups R5 are Phenyl, Fluorophenyl, Fluoropyridinyl, Methylphenyl, Dimethylphenyl.
Preferred groups R6 are Hydrogen, Fluoro, Chloro, C1-C3-Alkyl, C1-C3-Alkoxy.
Preferred groups R7 and R8 are hydrogen and the other is methyl or ethyl or in which both of R7 and R8 are methyl or ethyl.
In addition, the invention relates to the use of the compounds of general formula I for the production of pharmaceutical agents as well as their use for the production of pharmaceutical agents for treating inflammatory diseases.
The C1-C5-alkyl groups can be straight-chain or branched and stand for a methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, tert-butyl or n-pentyl group, or a 2,2-dimethylpropyl, 2-methylbutyl or 3-methylbutyl group. A methyl or ethyl group is preferred. They can optionally be substituted by 1-3 hydroxy, 1-3 C1-C5-alkoxy and/or 1-3 COOR6 groups. Preferred are hydroxy groups.
The C1-C5-alkoxy groups can be straight-chain or branched and stand for a methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy, iso-butoxy, tert-butoxy or n-pentoxy, 2,2-dimethylpropoxy, 2-methylbutoxy or 3-methylbutoxy group. A methoxy or ethoxy group is preferred.
The designation halogen atom or halogen means a fluorine, chlorine, bromine or iodine atom. Preferred is a fluorine, chlorine or bromine atom. More preferred is a fluorine or chlorine atom. Most preferred is a fluorine atom.
Due to the presence of asymmetry centers the compounds of general formula I according to the invention may have several stereoisomers. Subjects of this invention are all possible enantiomers and diastereomers, both as racemates and in enantiomer-pure form.
The compounds according to the invention can also be present in the form of salts with physiologically compatible anions, for example in the form of hydrochlorides, sulfates, nitrates, phosphates, pivalates, maleates, fumarates, tartrates, benzoates, mesylates, citrates or succinates.
The process for the production of the compounds of WO2005/034939, WO2006/027236, WO2006/066950 and WO2006/100100 can also be used for the production of the compounds according to the invention.
A) An aminoindazole or indole of general formula (II), in which, R1, R2, R3, R4, R6, R7 and R8 have the meanings that are indicated for formula (I), is converted with an arylboronic acid of the general formula (III), in which under R3 has the meaning that is indicated for formula (I), under catalysis of copper or palladium (P. Lam et al. Syn. Lett. 2000; 5, 674-76; H. Sano et al, Biorg. Med. Chem. 2005, 13, 3079-91) to compounds of the general formula (I) according to the invention.
B) Title compound (I) can also be synthesized with by imine formation of an aldehyde of general formula (IV), in which R1, R2, R3, R6, R7 and R8 have the meanings that are indicated for formula (I), and an amino-1-arylindazole or indole of general formula (V), in which Y and R4, R6 are defined as described above, according to the methods that are known to one skilled in the art, whereby, e.g., sodium cyanoborohydride, sodium triacetoxy borohydride, sodium borohydride or hydrogen is considered as a reducing agent under palladium catalysis and e.g. acetic acid or titanium tetra alcoholates are considered as acidic catalysts for the imine formation. The so formed imine can be treated with Lewis acid (e.g. BBr3 or TiCl4) at temperatures between (−50° C.) and (+20° C.) for the formation of the stereoisomer of formula I according to the invention.
If the compounds according to the invention are present as racemic mixtures, they can be separated into pure, optically active forms according to the methods of racemate separation that are familiar to one skilled in the art. For example, the racemic mixtures can be separated by chromatography on an even optically active carrier material (CHIRALPAK AD®) into the pure isomers. It is also possible to esterify the free hydroxy group in a racemic compound of general formula I with an optically active acid and to separate the diastereoisomeric esters that are obtained by fractionated crystallization or by chromatography, and to saponify the separated esters in each case to the optically pure isomers. As an optically active acid, for example, mandelic acid, camphorsulfonic acid or tartaric acid can be used.
The binding of the substances to the glucocorticoid receptor (GR) and other steroid hormone receptors (mineral corticoid receptor (MR), progesterone receptor (PR) and androgen receptor (AR)) is examined with the aid of recombinantly produced receptors. Cytosol preparations of Sf9 cells, which had been infected with recombinant baculoviruses, which code for the GR, are used for the binding studies. In comparison to reference substance [3H]-dexamethasone, the substances show a high to very high affinity to GR. Example 4 thus shows, e.g., the following profile: IC50(GR)=16.5 nmol; IC50(MR), IC50(PR), IC50(AR), IC50(ER)>1 μmol.
As an essential, molecular mechanism for the anti-inflammatory action of glucocorticoids, the GR-mediated inhibition of the transcription of cytokines, adhesion molecules, enzymes and other pro-inflammatory factors is considered. This inhibition is produced by an interaction of the GR with other transcription factors, e.g., AP-1 and NF-kappa-B (for a survey, see Cato, A. C. B., and Wade, E., BioEssays 18, 371-378, 1996).
The compounds of general formula I according to the invention inhibit the secretion of cytokine IL-8 into the human monocyte cell line THP-1 that is triggered by lipopolysaccharide (LPS). The concentration of the cytokines was determined in the supernatant by means of commercially available ELISA kits. The compound of Example 4 showed an inhibition IC50(IL8)=2.4 nM (91% eff. relative to dexamethasone).
The anti-inflammatory action of the compounds of general formula I was tested in the animal experiment by tests in the croton oil-induced inflammation in rats and mice (J. Exp. Med. (1995), 182, 99-108). To this end, croton oil in ethanolic solution was applied topically to the animals' ears. The test substances were also applied topically or systemically at the same time or two hours before the croton oil. After 16-24 hours, the ear weight was measured as a yardstick for inflammatory edema, the peroxidase activity as a yardstick for the invasions of granulocytes, and the elastase activity as a yardstick for the invasion of neutrophilic granulocytes. In this test, the compounds of general formula I inhibit the three above-mentioned inflammation parameters both after topical administration and after systemic administration.
One of the most frequent undesirable actions of a glucocorticoid therapy is the so-called “steroid diabetes” [cf., Hatz, H. J., Glucocorticoide: Immunologische Grundlagen, Pharmakologie und Therapierichtlinien [Glucocorticoids: Immunological Bases, Pharmacology and Therapy Guidelines], Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 1998]. The reason for this is the stimulation of gluconeogenesis in the liver by induction of the enzymes responsible in this respect and by free amino acids, which are produced from the degradation of proteins (catabolic action of glucocorticoids). A key enzyme of the catabolic metabolism in the liver is tyrosinamino transferase (TAT). The activity of this enzyme can be determined from liver homogenates by photometry and represents a good measurement of the undesirable metabolic actions of glucocorticoids. To measure the TAT induction, the animals are sacrificed 8 hours after the test substances are administered, the livers are removed, and the TAT activity is measured in the homogenate. In this test, at doses in which they have an anti-inflammatory action, the compounds of general formula I induce little or no tyrosinamino transferase.
Because of their anti-inflammatory and, in addition, anti-allergic, immunosuppressive and antiproliferative action, the compounds of general formula I according to the invention can be used as medications for treatment or prophylaxis of the following pathologic conditions in mammals and humans: In this case, the term “DISEASE” stands for the following indications:
Moreover, the compounds of general formula I according to the invention can be used for treatment and prophylaxis of additional pathologic conditions that are not mentioned above, for which synthetic glucocorticoids are now used (see in this respect Hatz, H. J., Glucocorticoide: Immunologische Grundlagen, Pharmakologie und Therapierichtlinien, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 1998).
All previously mentioned indications (i) to (xx) are described in more detail in Hatz, H. J., Glucocorticoide: Immunologische Grundlagen, Pharmakologie und Therapierichtlinien, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 1998. For the therapeutic actions in the above-mentioned pathologic conditions, the suitable dose varies and depends on, for example, the active strength of the compound of general formula I, the host, the type of administration, and the type and severity of the conditions that are to be treated, as well as the use as a prophylactic agent or therapeutic agent.
In addition, the invention provides:
In general, satisfactory results can be expected in animals when the daily doses comprise a range of 1 μg to 100,000 μg of the compound according to the invention per kg of body weight. In the case of larger mammals, for example the human, a recommended daily dose lies in the range of 1 μg to 100,000 μg per kg of body weight. Preferred is a dose of 10 to 30,000 μg per kg of body weight, and more preferred is a dose of 10 to 10,000 μg per kg of body weight. For example, this dose is suitably administered several times daily. For treating acute shock (e.g., anaphylactic shock), individual doses can be given that are significantly above the above-mentioned doses.
The formulation of the pharmaceutical preparations based on the new compounds is carried out in a way that is known in the art by the active ingredient being processed with the vehicles that are commonly used in galenicals, fillers, substances that influence decomposition, binding agents, moisturizers, lubricants, absorbents, diluents, flavoring correctives, coloring agents, etc., and converted into the desired form of administration. In this case, reference is made to Remington's Pharmaceutical Science, 15th Edition, Mack Publishing Company, East Pennsylvania (1980).
For oral administration, especially tablets, coated tablets, capsules, pills, powders, granulates, lozenges, suspensions, emulsions or solutions are suitable.
For parenteral administration, injection and infusion preparations are possible.
For intra-articular injection, correspondingly prepared crystal suspensions can be used.
For intramuscular injection, aqueous and oily injection solutions or suspensions and corresponding depot preparations can be used.
For rectal administration, the new compounds can be used in the form of suppositories, capsules, solutions (e.g., in the form of enemas) and ointments both for systemic and for local treatment.
For pulmonary administration of the new compounds, the latter can be used in the form of aerosols and inhalants.
For local application to eyes, outer ear channels, middle ears, nasal cavities, and paranasal sinuses, the new compounds can be used as drops, ointments and tinctures in corresponding pharmaceutical preparations.
For topical application, formulations in gels, ointments, fatty ointments, creams, pastes, powders, milk and tinctures are possible. The dosage of the compounds of general formula I should be 0.01%-20% in these preparations to achieve a sufficient pharmacological action.
The invention also comprises the compounds of general formula I according to the invention as therapeutic active ingredients.
In addition, the compounds of general formula I according to the invention are part of the invention as therapeutic active ingredients together with pharmaceutically compatible and acceptable adjuvants and vehicles.
The invention also comprises a pharmaceutical composition that contains one of the pharmaceutically active compounds according to the invention or mixtures thereof or a pharmaceutically compatible salt thereof and a pharmaceutically compatible salt or pharmaceutically compatible adjuvants and vehicles.
The compounds of general formula (I) according to the invention can optionally also be formulated and/or administered in combination with other active ingredients.
The invention therefore also relates to combination therapies or combined compositions, in which a compound of general formula (I) or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition that contains a compound of general formula (I) or a pharmaceutically acceptable salt thereof, is administered either simultaneously (optionally in the same composition) or in succession together with one or more pharmaceutical agents for treating one of the above-mentioned pathologic conditions. For example, for treatment of rheumatoid arthritis, osteoarthritis, COPD (chronic obstructive lung disease), asthma or allergic rhinitis, a compound of general formula (I) of this invention can be combined with one or more pharmaceutical agents for treating such a condition. When such a combination is administered by inhalation, the pharmaceutical agent that is to be combined can be selected from the following list:
For another subject of this invention, such a combination with a compound of general formula (I) or a pharmaceutically acceptable salt thereof is used for treatment of COPD, asthma or allergic rhinitis and can be administered by inhalation or orally in combination with xanthine (such as, for example, aminophylline or thyeophylline), which also can be administered by inhalation or orally.
The various aspects of the invention described in this application are illustrated by the following examples which are not meant to limit the invention in any way.
0.5 g (3.06 mmol) of 4-nitroindazole (Chem. Ber. 37, 1904, 2583) 860 mg (6.13 mmol) of 4-fluorophenyl boronic acid and 558 mg (2.79 mmol) copper acetate are stirred in 0.5 ml pyridine and 20 ml dichloromethane for 16 hours. The reaction mixture is treated with 5 g of silica gel and remaining solvent is removed in a vacuum. After chromatography on silica gel with hexane-ethyl acetate (50-100%), 600 mg of 4-nitro-1-(4-fluorophenyl)indazole is obtained. 300 mg (1.17 mmol) of 4-nitro-1-(4-fluorophenyl)indazole is dissolved in 10 ml methanol, 5 ml 2-propanole and 10 ml THF. 100 mg of Raney nickel is added, and the mixture is shaken for 3 hours under a hydrogen atmosphere at normal pressure. Catalyst is removed from the solution by means of filtration on Celite, whereby washing is continued with methanol and ethyl acetate, and it is concentrated by evaporation. After chromatography on silica gel with ethyl acetate-ethanol (0-10%), 240 mg of the desired 4-Amino-1-(4-fluorophenyl)indazole is obtained. 1H-NMR (CDCl3); δ=4.22 (br, 2H), 6.43 (d, 1H), 7.05 (d, 1H), 7.21 (dd, 2H), 7.22 (dd, 1H), 7.68 (dd, 2H), 8.13 (s, 1H).
0.28 ml (0.88 mmol) of titanium tetra tert-butylate and 0.05 ml of acetic acid are added to 136 mg (0.44 mmol) of 4-(3-fluoro-2-methoxyphenyl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal (WO2005/034939) and 100 mg (0.44 mmol) of 4-amino-1-(4-fluorophenyl)indazole in 11.5 ml of toluene, and the mixture is heated over 2.5 hours to 100° C. After cooling, it is poured into water, and vigorous stirring is continued. The suspension is filtered through Celite, and is thoroughly rewashed with ethyl acetate. The phases of the filtrate are separated, and it is extracted again with ethyl acetate. It is dried on sodium sulfate, and the solvent is removed in a vacuum to yield 220 mg crude 4-(3-fluoro-2-methoxyphenyl)-1,1,1-trifluoro-2-{[(4-fluorophenyl)indazol-4-yl]iminomethyl}-4-methyl-pentan-2-ol. The crude Imine is taken up in 12 ml of CH2Cl2, is mixed at −30° C. with 3.5 ml of 1 M boron tribromide solution in CH2Cl2. The mixture is warmed to −20° over 1 hours and stirred for additional 2 hours while warming to 0° C. The batch is poured into saturated NaHCO3 and ice, stirred for 20 minutes and extracted with ethyl acetate. The combined organic extracts are washed with brine, dried (Na2SO4) and concentrated by evaporation in a vacuum. After chromatography on silica gel with hexane-2-propanol (17%), 8 mg of the desired product and 13 mg of the methyl ether cleaved compound (example 2) are obtained. 1H-NMR (CDCl3); δ=1.53 (s, 3H), 1.66 (s, 3H), 2.07 (d, 1H), 2.17 (d, 1H), 3.98 (s, 3H), 5.12 (br., 1H), 6.51 (d, 1H), 6.92 (dd, 1H), 7.06 (dd, 1H), 7.07 (d, 1H), 7.23 (dd, 2H), 7.32 (t, 1H), 7.69 (dd, 2H), 8.21 (br., 1H).
Is obtained as additional product as described in example 1. 1H-NMR (CDCl3); δ=1.58 (s, 3H), 1.69 (s, 3H), 2.09 (d, 1H), 2.19 (d, 1H), 5.11 (s, 1H), 6.50 (d, 1H), 6.91 (m, 2H), 7.07 (d, 1H), 7.22 (dd, 1H), 7.31 (t, 1H), 7.68 (dd, 2H), 8.11 (s, 1H).
29.04 g (3-fluoro-2-methoxyphenyl)-boronic acid, 25 g 2-bromo-1-butene and 1.65 g tetrakis (triphenylphosphine) palladium (0) are dissolved in 174 mL toluene and 17.4 mL 1-propanol. The mixture is heated to 120° C. over 5 hours in a closed vial and poured into water after cooling. The aqueous phase is three times extracted with diethyl ether. The combined organic extracts are washed once with brine and dried over Na2SO4. The solvent is carefully distilled off (volatile product!) and the residue is purified by chromatography on silicagel (eluent hexane/diethyl ether), yielding 16.6 g 1-fluoro-2-methoxy-3-(1-methylenepropyl)benzene. To 12.5 g (69.3 mmol) of 1-fluoro-2-methoxy-3-(1-methylenepropyl)benzene and 7.5 g molecular sieve in 18.3 mL (138.6 mmol) ethyl trifluoropyruvate at 0° C. are added over 30 minutes 3.0 g (3.5 mmol) [Cu(4R,4R)-2,2-bis-(4-tert-butyl-2-oxazolin-2-yl)propane(H2O)2]((SbF6)2, dissolved in 90 mL dichloromethan. The reaction mixture is stirred for 16 hours at 0° C. and directly purified by chromatography on silica gel (eluent ethyl acetate/hexane) giving 18.6 g of the desired enantiomerically enriched ethyl (2R)-4-(3-fluoro-2-methoxyphenyl)-2-hydroxy-2-(trifluoromethyl)hex-4-enoate as an E/Z mixture. 9.3 g (2R,4E/Z) ethyl 4-(3-fluoro-2-methoxyphenyl)-2-hydroxy-2-(trifluoromethyl)hex-4-enoate are dissolved in 300 mL diethyl ether under argon and cooled to −15° C. 2.02 g lithium aluminum hydride are added over a period of 30 minutes in ten portions as solid and the resulting solution is stirred for one hour allowing the temperature rise to −5° C. After stirring for 30 minutes at −5° C., 4 mL ethyl acetate are added carefully and the mixture is stirred for 10 minutes. The reaction mixture is poured into a mixture of ice and ammonium acetate solution and stirred vigorously for 10 minutes. After multiple extractions with ethyl acetate and diethyl ether the combined organic extracts are washed once with brine and dried over Na2SO4. The solvent is distilled off and the residue is purified by chromatography on silicagel (hexane/diethyl ether), yielding 5.9 g (72.6%) (E/Z)-4-(3-fluoro-2-methoxyphenyl)-2-hydroxy-2-(trifluoromethyl)hex-4-enal and 2.0 g (25.6%) (E/Z)-4-(3-fluoro-2-methoxyphenyl)-2-(trifluoromethyl)hex-4-en-1,2-diol.
1.51 g (4.9 mmol) (E/Z)-4-(3-Fluor-2-methoxy-phenyl)-2-hydroxy-2-(trifluoromethyl)hex-4-enal are dissolved in 40 mL methanol and 1.2 mL acetic acid and 80 mg palladium on charcoal 10% are added under argon. The mixture is shaken under a hydrogen atmosphere until 150 mL hydrogen are absorbed. The suspension is filtered through a short path of celite, which is washed carefully with ethyl acetate and methanol. The solvent is distilled off and the residue is purified by chromatography on silicagel (eluent: hexane/diisopropylether), yielding 530 mg of the enantiomerically enriched (2R,4R)-4-(3-Fluor-2-methoxy-phenyl)-2-hydroxy-2-(trifluormethyl)hexanal 1H-NMR (300 MHz, CDCl3); δ=0.77 (d, 3H), 1.65 (m, 2H), 2.32 (dd, 1H), 2.55 (dd, 1H), 3.14 (m, 1H), 3.91 (s, 3H), 3.97 (s, 1H), 6.86 (dd, 1H), 6.95-6.99 (m, 2H), 8.99 (s, 1H) and 620 mg of (2R,4S)-4-(3-Fluor-2-methoxyphenyl)-2-hydroxy-2-(trifluoromethyl)hexanal 1H-NMR (300 MHz, CDCl3); δ=0.73 (d, 3H), 1.60 (m, 2H), 2.35 (dd, 1H), 2.43 (dd, 1H), 2.96 (m, 1H), 3.63 (s, 1H), 3.92 (s, 3H), 6.84 (dd, 1H), 6.93-6.99 (m, 2H), 9.67 (s, 1H) as a mixture.
0.20 ml (0.64 mmol) of titanium tetra t-butylate and 0.05 ml of acetic acid are added to 100 mg (0.32 mmol) of (2R,4R/S) 4-(3-fluoro-2-methoxyphenyl)-2-hydroxy-2-(trifluoromethyl)hexanal and 60 mg (0.26 mmol) of 4-amino-1-(4-fluorophenyl)indazole in 5 ml of toluene, and the mixture is heated over 2.5 hours to 100° C. After cooling, it is poured into water, and vigorous stirring is continued. The suspension is filtered through Celite, and is thoroughly rewashed with ethyl acetate. The phases of the filtrate are separated, and it is extracted again with ethyl acetate. It is dried on sodium sulfate, and the solvent is removed in a vacuum to yield 180 mg crude 4-(3-fluoro-2-methoxyphenyl)-1,1,1-trifluoro-2-{[(4-fluorophenyl)indazol-4-yl]iminomethyl}hexan-2-ol. The crude Imine is taken up in 5 ml of CH2Cl2, is mixed at −30° C. with 3.5 ml of 1 M boron tribromide solution in CH2Cl2. The mixture is warmed to −20° over 1 hours and stirred for additional 2 hours while warming to 0° C. The batch is poured into saturated NaHCO3 and ice, stirred for 20 minutes and extracted with ethyl acetate. The combined organic extracts are washed with brine, dried (Na2SO4) and concentrated by evaporation in a vacuum. After chromatography on silica gel with hexane-2-propanol (17%) 21 mg of the desired product 1H-NMR (CDCl3); δ=1.13 (t, 3H), 1.92 (ddq, 1H), 2.05 (m, 1H), 2.14 (dd, 1H), 2.41 (dd, 1H), 3.18 (m, 1H), 5.12 (s, 1H), 6.62 (d, 1H), 6.74 (dd, 1H), 6.87 (dd, 1H), 7.14 (d, 1H), 7.21 (dd, 2H), 7.34 (t, 1H), 7.66 (dd, 2H), 8.11 (s, 1H) and 42 mg of the (8R)-diastereomer are obtained.
Is obtained as additional product as described in example 3 1H-NMR (CDCl3); δ=0.95 (t, 3H), 1.79 (m, 1H), 1.97 (ddq, 1H), 2.14 (dd, 1H), 2.36 (dd, 1H), 3.38 (m, 1H), 5.03 (s, 1H), 6.34 (d, 1H), 6.92 (dd, 1H), 6.98 (dd, 1H), 7.03 (d, 1H), 7.22 (dd, 2H), 7.27 (t, 1H), 7.68 (dd, 2H), 8.12 (s, 1H).
20 mg (0.07 mmol) of cis-2-Chloro-5,6,7,8-tetrahydro-5-[(indazol-4-yl)amino]-8,8-dimethyl-6-(trifluoromethyl)naphthalene-1,6-diol (WO 2005/034939), 19.9 mg (0.14 mmol) 6-fluoropyridin-3-yl boronic acid and 8.5 mg (0.043 mmol) copper acetate are stirred in 0.011 ml pyridine and 2 ml dichloromethane for 16 hours. The reaction mixture is directly transferred to preparative silica gel thin layer plates (20×20 cm). After thin layer chromatography with hexane-ethyl acetate (50%), 5 mg of the desired product is obtained. 1H-NMR (CDCl3); δ=1.57 (s, 3H), 1.70 (s, 3H), 2.11 (d, 1H), 2.21 (d, 1H), 5.12 (s, 1H), 5.98 (s, 1H), 6.53 (d, 1H), 6.95 (d, 1H), 7.08 (d, 1H), 7.13 (dd, 1H), 7.15 (dd, 1H), 7.36 (dd, 1H), 8.17 (s, 1H), 8.18 (ddd, 1H), 8.63 (m, 1H).
20 mg (0.07 mmol) of cis-2-Chloro-5,6,7,8-tetrahydro-5-[(indazol-4-yl)amino]-8,8-dimethyl-6-(trifluoromethylnaphthalen-1,6-diol (WO 2005/034939), 19.7 mg (0.14 mmol) 6-fluoropyridin-3-yl boronic acid and 8.5 mg (0.043 mmol) copper acetate are stirred in 0.011 ml pyridine and 2 ml dichloromethane for 16 hours. The reaction mixture is directly transferred to preparative silica gel thin layer plates (20×20 cm). After thin layer chromatography with hexane-ethyl acetate (50%), 10 mg of the desired product is obtained. 1H-NMR (CDCl3); δ=1.58 (s, 3H), 1.70 (s, 3H), 2.10 (d, 1H), 2.20 (d, 1H), 5.12 (br., 1H), 5.97 (s, 1H), 6.50 (d, 1H), 6.94 (d, 1H), 7.08 (d, 1H), 7.14 (d, 1H), 7.22 (dd, 2H), 7.31 (t, 1H), 7.69 (dd, 1H), 8.10 (s, 1H).
0.5 g (3.06 mmol) of 4-nitroindazole (Chem. Ber. 37, 1904, 2583) 860 mg (6.13 mmol) of (6-fluoropyridin-3-yl)boronic acid and 557 mg (2.79 mmol) copper acetate are stirred in 0.5 ml pyridine and 20 ml dichloromethane for 16 hours. The reaction mixture is treated with 5 g of silica gel and remaining solvent is removed in a vacuum. After chromatography on silica gel with hexane-ethyl acetate (50-100%), 280 mg of 4-nitro-1-(6-fluoropyridin-3-yl)indazole is obtained. 280 mg (1.08 mmol) of 4-nitro-1-(6-fluoropyridin-3-yl)indazole is dissolved in 8 ml methanol, 4 ml 2-propanole and 8 ml THF. 50 mg of Raney nickel is added, and the mixture is shaken for 3 hours under a hydrogen atmosphere at normal pressure. The catalyst is removed from the solution by means of filtration on Celite, whereby washing is continued with methanol and ethyl acetate, and it is concentrated by evaporation. After chromatography on silica gel with hexan-ethyl acetate (50%), 180 mg of the desired 5-Amino-1-(6-fluoropyridin-3-yl)indazole is obtained. 1H-NMR (CDCl3); δ=6.47 (d, 1H), 7.05 (d, 1H), 7.12 (dd, 1H), 7.27 (dd, 1H), 8.16 (ddd, 1H), 8.18 (s, 1H), 8.62 (m, 1H).
(2R,4R)-4-(3-Fluor-2-methoxy-phenyl)-2-hydroxy-2-(trifluormethyl)hexanal and 4-amino-1-(6-fluoropyridin-3-yl)indazole are reacted, as described in Example 4, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[(6-fluoropyridin-3-yl)1H-indazole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
Analogously to example 5, 2-fluoro-5,6,7,8-tetrahydro-5-[1H-indazol-4-yl)amino]-8,8-dimethyl-6-(trifluoromethyl)naphthalene-1,6-diol (WO 2005/034939) can be reacted with 6-fluoropyridin-3-yl boronic acid under copper acetate catalysis.
(2R,4R)-4-(3-Fluor-2-methoxy-phenyl)-2-hydroxy-2-(trifluormethyl)hexanal and 4-amino-1-(4-fluorophenyl)-1H-indole are reacted, as described in Example 4, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[1-(4-fluorophenyl)-1H-indole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
(2R,4R)-4-(3-Fluor-2-methoxy-phenyl)-2-hydroxy-2-(trifluormethyl)hexanal and 4-amino-1-(6-fluoropyridin-3-yl)-1H-indole are reacted, as described in Example 4, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[1-(6-fluoropyridinyl)-1H-indole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra tert-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
Analogously to example 5, 2-fluoro-5,6,7,8-tetrahydro-5-[(1H-indazol-4-yl)amino]-8,8-dimethyl-6-(trifluoromethyl)naphthalene-1,6-diol (WO 2005/034939) can be reacted with 2-fluoropyridin-4-yl boronic acid under copper acetate catalysis.
4-(3-Fluor-2-methoxyphenyl)-2-hydroxy-3-methyl-2-(trifluormethyl)pentanal (WO2006/100100) and 4-amino-1-(6-fluoropyridin-3-yl)indazole are reacted, as described in Example 4, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[(4-fluorophenyl)1H-indazole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
4-(3-Fluor-2-methoxyphenyl)-2-hydroxy-3-methyl-2-(trifluormethyl)pentanal and 4-amino-1-(3-fluorophenyl)indazole are reacted, as described in Example 4, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[(3-fluorophenyl)-1H-indazole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
4-(3-Fluor-2-methoxyphenyl)-2-hydroxy-3-methyl-2-(trifluormethyl)pentanal and 4-amino-1-(4-fluorophenyl)-1H-indole are reacted, as described in Example 3, to form 4-(3-fluoro-2-methoxyphenyl)-1-{[(4-fluorophenyl)-1H-indole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t.-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
Can be prepared analogously to Example 3 g (2R,4R)-4-(3,4-Difluor-2-methoxyphenyl)-2-hydroxy-2-(trifluormethyl)hexanal 1H-NMR (300 MHz, CDCl3); δ=0.75 (t, 3H), 1.55-1.73 (m, 2H), 2.30 (dd, 1H), 2.54 (dd, 1H), 3.06 (m, 1H), 3.92 (s, 1H), 3.96 (s, 3H), 6.75-6.84 (m, 2H), 9.02 (s, 1H).und 3.9 g (2R,4S)-4-(3,4-Difluor-2-methoxyphenyl)-2-hydroxy-2-(trifluormethyl)hexanal 1H-NMR (300 MHz, CDCl3); δ 0.71 (t, 3H), 1.50-1.70 (m, 2H), 2.33 (dd, 1H), 2.41 (dd, 1H), 2.87 (m, 1H), 3.60 (s, 1H), 3.95 (s, 3H), 6.75-6.86 (m, 2H), 9.69 (s, 1H).
4-(3,4-Difluoro-2-methoxy-phenyl)-2-hydroxy-2-(trifluormethyl)hexanal and 4-amino-1-(6-fluoropyridin-3-yl)indazole are reacted, as described in Example 3, to form 4-(3,4-di-fluoro-2-methoxyphenyl)-1-{[(4-fluorophenyl)1H-indazole-4-yl]imino}-2-(trifluoromethyl)hexan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with borontribromide in dichloromethane yields the desired product after chromatography on silica gel.
4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal (WO2005/034939) and 4-amino-1-(6-fluoropyridin-3-yl)-1H-indazole are reacted, as described in Example 3, to form 4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-1-{[(3,5-dimethylphenyl)indazole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal and 4-amino-1-(4-fluorophenyl)-1H-indole (Biorg. Med. Chem. 2005, 13, 3079-91) are reacted, as described in Example 3, to form 4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-1-{[(3,5-dimethylphenyl)indazole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
Analogously to Example 5, cis-6-Chloro-1,2,3,4-tetrahydro-1-[(indazol-5-yl)amino]-5-methoxy-4,4-dimethyl-2-(trifluoromethyl)naphthalene-2-ol can be reacted with 4-fluorophenyl boronic acid under copper acetate catalysis.
Can be obtained from cis-6-Choro-1-{[1-(4-fluorophenyl)-1H-indazol-5-yl]amino}-1,2,3,4-tetrahydro-5-methoxy-4,4-dimethyl-6-(trifluoromethyl)naphthalene-2-ol by treatment with boron tribromide.
Analogously to Example 5, cis-6-Chloro-1,2,3,4-tetrahydro-1-[(indazol-5-yl)amino]-5-methoxy-4,4-dimethyl-2-(trifluoromethyl)naphthalene-2-ol can be reacted with 4-methylphenyl boronic acid under copper acetate catalysis.
Can be obtained cis-6-Choro-1,2,3,4-tetrahydro-5-methoxy-4,4-dimethyl-1-{[1-(4-methylphenyl)-1H-indazol-5-yl]amino}-6-(trifluoromethyl)naphthalene-2-ol by treatment with boron tribromide.
Analogously to Example 5, cis-1,2,3,4-tetrahydro-1-[(indazol-5-yl)amino]-5-methoxy-4,4-dimethyl-6-iso-propyl-2-(trifluoromethyl)naphthalene-2-ol can be reacted with 4-fluorophenyl boronic acid under copper acetate catalysis.
Can be obtained from cis-1-{[1-(4-fluorophenyl)-1H-indazol-5-yl]amino}-1,2,3,4-tetrahydro-5-methoxy-4,4-dimethyl-2-iso-propyl-6-(trifluoromethyl)naphthalene-2-ol by treatment with boron tribromide.
Analogously to Example 5, cis-1,2,3,4-tetrahydro-1-[(indazol-5-yl)amino]-5-methoxy-4,4-dimethyl-6-iso-propyl-2-(trifluoromethyl)naphthalene-2-ol can be reacted with 4-fluorophenyl boronic acid under copper acetate catalysis.
Can be obtained from cis-1-{[1-(4-fluorophenyl)-1H-indazol-5-yl]amino}-1,2,3,4-tetrahydro-5-methoxy-4,4-dimethyl-2-iso-propyl-6-(trifluoromethyl)naphthalene-2-ol by treatment with boron tribromide.
4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal (WO2005/034939) and 5-amino-1-(3,5-dimethylphenyl)-1H-indole (Biorg. Med. Chem. 2005, 13, 3079-91) are reacted, as described in Example 3, to form 4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-1-{[(3,5-dimethylphenyl)indole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
(2,3-Dihydrobenzofuran-7-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal and 5-amino-1-(3,5-dimethylphenyl)-1H-indole are reacted, as described in Example 3, to form (2,3-dihydro-5-fluorobenzofuran-7-yl)-2-hydroxy-4-methyl-1-{[(3,5-dimethylphenyl)-1H-indole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
2 g (12.3 mmol) of 5-nitroindazole, 3.43 g (24.52 mmol) of 4-fluorophenyl boronic acid, 9.2 g molecular sieves and 3.67 g (18.9 mmol) copper acetate are stirred in 2 ml pyridine and 140 ml dichloromethane for 16 hours. The reaction mixture is treated with 15 g of silica gel and remaining solvent is removed in a vacuum. After chromatography on silica gel with hexane-ethyl acetate (10-30%) and recrystallisation in hexane-ethyl acetate (30%) 1.09 g of 5-nitro-1-(4-fluorphenyl)-indazole is obtained. 4.88 g (19 mmol) of 5-nitro-1-(4-fluorophenyl)indazole is suspended in 107 ml ethanol, 107 ml dioxane and 22.7 ml saturated NH4Cl solution. 15.3 g of Indium powder is added, and the mixture is stirred for 20 hours at 100° C. The reaction mixture is diluted with and solids are removed from the solution by means of filtration on Celite at 50° C., whereby washing is continued with methanol and CH2Cl2, and it is concentrated by evaporation. After chromatography on silica gel with hexane-ethyl acetate (10%-100%), 2.3 g of the desired 5-Amino-1-(4-fluorophenyl)indazole is obtained. 1H-NMR (CDCl3); δ=3.67 (br, 2H), 6.90 (d, 1H), 6.99 (s, 1H), 7.21 (dd, 2H), 7.52 (d, 1H), 7.66 (dd, 2H), 7.99 (s, 1H).
4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal and 5-amino-1-(4-fluorophenyl)-1H-indazole are reacted, as described in Example 3, to form 4-(1,3-Benzodioxol-4-yl)-2-hydroxy-4-methyl-1-{[(4-fluorophenyl)indazole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
(2,3-Dihydrobenzofuran-7-yl)-2-hydroxy-4-methyl-2-(trifluoromethyl)pentanal and 5-amino-1-(4-fluorophenyl)-1H-indole are reacted, as described in Example 3, to form (2,3-dihydro-5-fluorobenzofuran-7-yl)-2-hydroxy-4-methyl-1-{[(4-fluorophenyl)-1H-indazole-5-yl]imino}-4-methyl-2-(trifluoromethyl)pentan-2-ol in the presence of titanium tetra t-butylate and acetic acid. Further treatment of the crude imine with titanium tetrachloride in dichloromethane yields the desired product after chromatography on silica gel.
Without further elaboration, it is believed that one skilled in the art can, using the preceding description, utilize the present invention to its fullest extent. The preceding preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
In the foregoing and in the examples, all temperatures are set forth uncorrected in degrees Celsius and, all parts and percentages are by weight, unless otherwise indicated.
The entire disclosures of all applications, patents and publications, cited herein and of corresponding European application No. 06076960.1, filed Oct. 31, 2006, and U.S. Provisional Application Ser. No. 60/855,380, filed Oct. 31, 2006, are incorporated by reference herein.
The preceding examples can be repeated with similar success by substituting the generically or specifically described reactants and/or operating conditions of this invention for those used in the preceding examples.
From the foregoing description, one skilled in the art can easily ascertain the essential characteristics of this invention and, without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.
| Number | Date | Country | Kind |
|---|---|---|---|
| EP 06076960.1 | Oct 2006 | EP | regional |
This patent applications claims the priority according to the Paris Convention of European Patent application No. EP 06076960.1 filed Oct. 31, 2006 as well as the benefit of the U.S. provisional application 60/855,380 filed Oct. 31, 2006, both of which are incorporated herein by reference.
| Number | Date | Country | |
|---|---|---|---|
| 60855380 | Oct 2006 | US |
