D29 shuttle phasmids and uses thereof

Abstract

The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of interest into the chromosome of mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a conditional shuttle plasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and method of generating auxotrophic mutants. Finally, the present invention provides a method of inactivating a mycobacterial virulence gene.

Description

BACKGROUND OF THE INVENTION

Tuberculosis (which includes infection caused by M. tuberculosis, M. bovis, BCG and M. africanum) remains the largest cause of human death in the world from a single infectious disease, and is responsible for one in four avoidable adult deaths in developing countries. In addition, in 1990, there was a 10% increase in the incidence of tuberculosis in the United States.

In the past, infection with drug-sensitive strains of the M. tuberculosis complex had been cured with certain antibiotics, including isoniazid, rifampicin, ethionamide and pyrazinamide. However, resistance to isoniazid and other antibiotic drugs has developed in many strains of M. tuberculosis. This has resulted in the search for an effective vaccine against M. tuberculosis. Further, this has enhanced the need to develop new drugs which are effective against drug-resistant strains of M. tuberculosis. It is therefore desirable to develop molecular and genetic tools which can be utilized to understand the pathways involved in invasion, survival and persistence of M. tuberculosis and in the development of vaccines and new drugs.

The creation of mutants in M. tuberculosis and BCG is of essential importance in the analysis of M. tuberculosis and BCG gene function. Auxotrophic mutants have been isolated in M. smegmatis by both shuttle mutagenesis and N-methylN'-nitroso-N-nitrosoguanidine treatment followed by isoniazid enrichment. These methods, however, are less effective in the M. tuberculosis complex (M. tuberculosis, M. bovis, M. miroti and M. africanum) due to current difficulties in performing homologous recombination, which is required by the shuttle mutagenesis procedure. Also, the tendency of mycobacteria to clump limits the use of traditional mutagens and makes positive selection advantageous.

Because the creation of mutants in M. tuberculosis and BCG is of essential importance in the analysis of gene function, it is desirable to develop effective means and methods for delivering foreign DNA into M. tuberculosis and BCG. The insertion of foreign DNA into M. tuberculosis and BCG mycobacteria would provide the necessary tools for understanding the mechanisms by which these mycobacteria survive and replicate. In addition, it would provide valuable tools for the development of vaccines and new drugs effective in the treatment of infection caused by M. tuberculosis and BCG.

The inventors of the present invention have constructed a class of novel cloning vectors for introducing DNA of interest into Mycobacterium tuberculosis complex organisms, i.e., M. tuberculosis, M. bovis and Bacille-Calmette-Geurin (BCG), and other slow growing mycobacteria as well as nontuberculosis mycobacteria commonly encountered in biological samples isolated from human subjects. The cloning vector of the present invention is a conditional shuttle plasmid constructed using the D29 mycobacteriophage.

SUMMARY OF THE INVENTION

The present invention provides a purified and isolated nucleic acid encoding D29 mycobacteriophage. The present invention also provides a conditional shuttle phasmid capable of introducing DNA of interest into a mycobacterium and a conditional shuttle phasmid which contains DNA of interest inserted into its genome.

The present invention further provides a recombinant mycobacterium which expresses DNA of interest introduced into its chromosome by a conditional shuttle phasmid containing the DNA of interest.

The present invention also provides an auxotrophic mutant produced by infecting a mycobacterium with a conditional shuttle plasmid containing a transposon such that the transposon is able to incorporate randomly into the chromosome of the mycobacterium thereby generating an auxotrophic mutation and an auxotrophic mutant produced by infecting a mycobacterium with a conditional shuttle plasmid containing a DNA of interest capable of inactivating a particular gene or genes of the mycobacterium.

Finally, the present invention provides a method for generating a library of mutant mycobacteria comprising infecting each member of a wild-type population of mycobacteria with a conditional shuttle phasmid containing a transposon incorporated into its genome at a non-permissive temperature for replication of the conditional shuttle plasmid such that said transposon is able to randomly insert itself into the chromosome of each member of the population of wild-type mycobacteria thereby randomly generating mutations in the mycobacteria as well as a method for inactivating a virulence gene in a mycobacterium comprising infecting the mycobacterium with a conditional shuttle phasmid containing a DNA of interest such that the DNA of interest can become incorporated into the chromosome of the mycobacteria and inactivating the virulence gene.

Additional objects of the present invention will be apparent from the description which follows.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 depicts a schematic representation of the conditional shuttle phasmid phAE70.

FIG. 2 depicts the effect of infection of mc.sup.2 155 cells with phAE78. phAE78 contains the transposon min Tn10. Kanamycin-resistant colonies can be observed on the plates on the left of FIG. 2. The control consisting of mc.sup.2 155 cells shows no kamamycin-resistant colonies.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides a novel class of cloning vectors based on the D29 mycobacteriophage for introducing DNA of interest into mycobacteria. The cloning vectors of the present invention are particularly suited for introducing DNA of interest into Mycobacterium tuberculosiscomplex organisms, i.e., M. tuberculosis, M. bovis and Bacille-Calmette-Geurin (BCG), and other slow growing mycobacteria as well as nontuberculosis mycobacteria commonly encountered in biological samples isolated from human subjects, e.g., M. avium-intracellulare, M.kansasii, M. xenopi, M. scrofulaceum, M. simiae, M. szulgai, M. gordonae, M. gastri, M. smegmatis, and M. chelonae.

The cloning vector of the present invention is a conditional shuttle phasmid which may be produced by exposing the shuttle phasmid formed by inserting a cosmid such as pYUB328 into a non-essential region of the D29 genome and isolating thermosensitive mutants formed thereby, i.e., mutants which are able to produce plaques on M. smegmatis at 30.degree. C. but not 37.degree. C.

In the preferred embodiment of the present invention, the cloning vector is the conditional shuttle phasmid designated phAE70 which was deposited under the terms of the Budapest Treaty on Mar. 7, 1996 with the American Type Culture Collection (ATCC), Rockville, Md., and assigned ATCC Accession No. 97468.

The conditional shuttle phasmid of the present invention may introduce DNA of interest into the chromosome of a mycobacterium by homologous recombination, site-specific recombination or nonhomologous recombination. The DNA of interest may be of any origin and may encode proteins or polypeptides of interest including but not limited to antigens, anti-tumor agents, enzymes, lymphokines, pharmacologic agents, immunopotentiators, and reporter molecules of interest.

Antigens include but are not limited to Mycobacterium leprae antigens; M.tuberculosis antigens; Rickettsea antigens; malaria sporozites and meroziotes; diptherea toxiods; tetanus toxoids; Clostridium antigens; Leishmania antigens; Salmonella antigens; Borrelia antigens; M. africanum antigens; M.intracellulare antigens; M.avium antigens; Treponema antigens; Pertussis antigens; Schistosoma antigens; Filaria antigens; Herpes virus antigens; influenza and parainfluenza virus antigens; measles virus antigens; mumps virus antigens; hepatitis virus antigens; rabies virus antigens; Shigella antigens; Neisseria antigens; polio virus antigens; Rift Valley Fever virus antigens; dengue virus antigens; Human Immunodeficiency Virus (HIV) antigens; respiritory syncytial virus (RSV) antigens; snake venom antigens; and Vibrio cholera antigens. Enzymes which may be encoded include but are not limited to steroid enzymes.

Anti-tumor agents which may be encoded include but are not limited to interferon-alpha, interferon-beta, or interferon-gamma, and tumor necrosis factor (TNF). Lymphokines which may be encoded include but are not limited to interleukins 1 through 8. Reporter molecules include but are not limited to luciferase (from Vibrio or from firefly), beta-galactosidase, beta-glucoronidase, and catechol dehydrogenase. Other peptides or proteins which may be encoded by the DNA of interest include but are not limited to stress proteins, which can be administered to evoke an immune response or to induce tolerance in an autoimmune disease, e.g., rheumatoid arthritis.

The conditional shuttle phasmid of the present invention may also be used to introduce transposons into the chromosome of a mycobacteria. As used herein, the term "transposon" is a general term which encompasses both non-mutated transposons and mutated transposons, i.e., a transposon in which a portion of the nucleotide sequence has been deleted and/or replaced, and/or wherein the transposon contains additional DNA sequences. In general a transposon contains an inverted repeat sequence at the 5' and 3' end, and a gene(s) encoding a transposase enzyme(s) between the inverted repeat sequences. The transposase(s) acts upon the inverted repeat sequences so as to enable the transposon to remove itself from the shuttle phasmid and insert or transpose into the chromosome of the mycobacteria. In some instances, the transposon may also include gene(s) encoding resolvase(s) and/or regulatory protein(s) which regulate transposition.

DNA of interest may be obtained from by a variety of methods, e.g., DNA may be isolated directly from an organism using standard purification procedures or by the cloning and amplification of the DNA of interest using methods known to one skilled in the art of genetic engineering such as PCR. Cloning of the DNA of interest into the conditional shuttle plasmid of the present invention may be accomplished using standard cloning and DNA ligation techniques. For example, the cohesive ends of the shuttle phasmid may be ligated using known ligase enzymes and the resulting molecule digested with a unique restriction endonuclease. Alternatively, a polylinker sequence containing recognition sites for a number of unique restriction enzymes may be inserted into the shuttle phasmid and the polylinker sequences digested with one or more restriction endonucleases in order to generate appropriate restriction sites on the shuttle phasmid to facilitate insertion of the DNA of interest. The use of two unique restriction endonucleases may be preferred for the directional cloning of the DNA of interest.

In addition to the DNA of interest, a selectable marker gene, e.g. a gene which confers antibiotic resistance, may be ligated to the DNA of interest and the resultant molecule ligated into the conditional shuttle phasmid using standard procedures (the conditional shuttle phasmid generally contains antibiotic resistance genes for selection of colonies in E. coli). Selectable marker genes which may be included on the DNA of interest are well known in the art and include but are not limited to genes encoding resistance to kanamycin, viomycin, thiostrepton, hygromycin or bleomycin.

The resulting ligated DNA comprising conditional shuttle phasmid DNA, DNA of interest and/or selectable marker DNA is then packaged into bacteriophage lambda heads using a commercially available in vitro packaging mix. E. coli is subsequently transduced with the phage colonies containing the ligated DNA are isolated by virtue of their ability to grow in medium containing the antibiotic corresponding to the resistance gene on the cosmid.

The resulting shuttle plasmids are introduced into M.smegmatis or other suitable mycobacteria using standard methods known to those skilled in the art such as electroporation. Plaques which contain shuttle phasmids comprising the DNA of interest may be used to infect mycobacteria of interest, e.g., M. tuberculosis and BCG.

Infection of mycobacteria such as M. tuberculosis with conditional shuttle phasmids containing the DNA of interest is accomplished by mixing the mycobacteria and shuttle phasmid at the non-permissive temperature at a multiplicity which will insure infection of mycobacterium. The calculation of the correct multiplicity is well within the knowledge of one skilled in the art. The phasmid DNA thus enters the mycobacterium but is unable to replicate. In this way the DNA of interest can become integrated into the mycobacterial chromosome and so expressed. Selection of mycobacteria containing the DNA of interest can be detected by virtue of their survival and growth on medium containing the antibiotic corresponding to the selectable markers encoded by the DNA of interest. Alternatively, mycobacteria expressing antigens or other proteins may also be detected by other methods, e.g., use of monoclonal antibodies.

The conditional shuttle plasmids of the present invention may also be used to generate libraries of mutated mycobacteria using conditional shuttle phasmids containing transposons such as Tn567 and mini Tn10 and methods detailed in the Experimental Details Section which follows or conditional shuttle phasmids containing DNA of interest, the expression of which will inactivate a specific mycobacterial gene or genes, e.g., antisense RNA.

Such methods are able to produce auxotrophic mutants, i.e., mutants which require a nutrient or substance not required by the organism from which the mutant was derived, e.g. leucine and other amino acids. Such non-pathogenic mycobacteria may be produced by insertional mutations or deletions causing disruptions of a mycobacterial virulence gene or by the expression of a molecule to block expression of the virulence gene, e.g. by knocking out genes in the mycobacteria which encode virulence factors, enzymes involved in regulation of pathways of the mycobacterium, and the like. Thus the conditional shuttle phasmid of the present invention may be used to generate a library of M.tuberculosis mutants that can be screened for their utility as vaccine strains.

The present invention also relates to methods of vaccinating a host with the recombinant mycobacterium to elicit protective immunity in the host. The recombinant vaccine can be used to produce humoral antibody immunity, cellular immunity (including helper or cytoxic immunity) and/or mucosoal or secretory immunity. In addition, the present invention relates to the use of the antigens expressed by the recombinant cultivable mycobacterium as vaccines or diagnostic reagents as described in WO 90/00594, which is incorporated by reference in its entirety.

Mycobacteria are well known adjuvant properties and so are able to stimulate a subject's immune response to respond to other antigens with great effectiveness. Their adjuvant properties are especially useful in providing immunity against pathogens in cases where cell mediated immunity is critical for resistance. In addition, the mycobacterium stimulates long-term memory or immunity thus a single inoculuum may be used to produce long-term sensitization to protein antigens. The mycobacterial vaccine of the present invention may be used to prime long-lasting T cell memory, which stimulates secondary antibody responses which will neutralize infectious agents or toxins, e.g., tetanus and diptherea toxins, pertusis, malaria, influenza, herpes viruses and snake venoms.

BCG is an especially appropriate vaccine vehicle in that it is the only childhood vaccine currently given at birth, it has a low incidence of adverse effects when given as a vaccine against tuberculosis and it can be used repeatedly in an individual. A further advantage of BCG and mycobacteria in general is the large size of their genomes (approximately 3.times.10.sup.6 bp). The large size enables DNA from other sources to be stably incorporated thus making multi-vaccine possible, i.e., a mycobacterium which expresses DNA of interest which encodes antigens from more than one pathogen.

The vaccines or therapeutic agents containing transformed mutated mycobacteria are administered in conjunction with a suitable pharmaceutical carrier. Mineral oil, alum, synthetic polymers are representative examples of suitable carriers. Vehicles for vaccines and therapeutic agents are well known in the art and the selection of a suitable vehicle is well within the skill of one skilled in the art. The selection of a suitable vehicle is also dependent upon the manner in which the vaccine or therapeutic agent is to be administered. The vaccine or therapeutic agent may be in the form of an injectable dose and may be administered intramuscularly, intravenously, orally, intradermally, or by subcutaneous administration.

The shuttle vectors of the present invention can also be used in the diagnosis of infection by mycobacteria. For example, a shuttle phasmid specific for M.tuberculosis or other type of mycobacteria may be ligated to a reporter molecule, e.g., luciferase from Vibrio or of firefly origin; beta-galactosidase; beta-glucoronidase; or catechol dehydrogenase and a strong mycobacterial promoter which controls expression of the reporter molecule-encoding gene. A sample from a subject suspected of suffering from an infection can be tested. The sample is cultured and combined with the specific phage. After a short time, e.g., hours, under appropriate conditions, the sample is assayed using known techniques, to detect the reporter molecule encoded by the DNA in the vector.

Mycobacteria can also be used as a source of a protein encoded by a DNA of interest. The DNA of interest may have a signal sequence added, e.g., from alpha antigen, which is secreted in mycobacteria, beta-galactosidase, agarase or alpha amylase in order to facilitate transport of the protein out of the mycobacterial cell.

The present invention is described in the following Experimental Details Section which is set forth to aid in the understanding of the invention, and should not be construed to limit in any way the scope of the invention as defined in the claims which follow thereafter.

EXPERIMENTAL DETAILS SECTION

I. Development of D29 Conditional Shuttle Plasmid

Shuttle plasmid designated phAE65 was constructed by inserting the Pac1 cosmid pYUB328 into the non-essential region of mycobacteriophage D29 (the entire nucleotide sequence of D29 is shown below) using methods described by Jacobs, et al., Nature, 327:532-536 (1987). D29 infects M. tuberculosis and M. smegmatis.

phAE65 was subjected to hydroxylamine mutagenesis and thermosensitive mutants, designated phAE70 through phAE76, that could plaque M. smegmatis at 30.degree. C. but failed to plaque M. smegmatis at 37.degree. C. were isolated. Mutations in early phage infection functions were screened for by selecting phages that failed to kill M. smegmatis at the non-permissive temperature. FIG. 1 depicts a schematic representation of the conditional shuttle phasmid phAE70 which was deposited with the ATCC, Rockville, Md., on Mar. 7, 1996 and assigned ATTCC Accession No. 97468.

A minimally sized cosmid, pYUB552, whose size of 2.7 kb after packaging made it possible to clone an additional 4.4 kb of DNA into the vector was cloned into phAE70. The cosmid portion of the conditional shuttle phasmids exemplified by phAE70 may be completely excised by digestion of the conditional shuttle phasmid with Pac1.

II. Construction of Conditional Shuttle Phasmids Containing a Transposon

Transposons Tn5367 and mini Tn10 d aph were cloned into pYUB552 using standard methods to generate pYUB553 and pYUB554, respectively. Using standard cosmid cloning technology in E. coli cosmid pYUB328 was replaced by the cosmids pYUB553 (containing Tn5367) and pYUB554 (containing mini Tn10 d aph) to generate shuttle plasmids phAE77 and phAE78, respectively. Titers of D29-packaged shuttle phasmids in excess of 5.times.10.sup.10 were prepared following transfection of M. smegmatis at 30.degree. C.

III. Infection of mc.sup.2 155 With Conditional Shuttle Phasmids containing Transposons

M. smegmatis mc.sup.2 155 cells were infected at a multiplicity of 10 with phAE77 and phAE78 at the non-permissive temperature. A multiplicity of infection of 10 was used in order to ensure that every cell in the population was infected with a conditional shuttle phasmid. Infection of the cells occurred at the non-permissive temperature. Therefore the the phasmids were able to enter the cell but were unable to replicate.

For M. smegmatis greater than 10.sup.4 kanamycin-resistant colonies per 10.sup.9 mc.sup.2 155 cells were observed using phAE78 (see FIG. 2) and greater than 1000 kanamycin-resistant colonies per 108 cells were observed with BCG cells and phAE77. In both cases control plates consisting of cells alone yielded no kanamycin-resistant colonies (see FIG. 2). Since the gene for kanamycin-resistance is carried on the transposon it was concluded that the kanamycin-resistant colonies are the result of transpositions.

Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of various aspects of the invention. Thus it is to be understood that numerous modifications may be made in the illustrative embodiments and other arrangements may be devised without departing from the spirit and scope of the invention in the appended claims.

__________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 1(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 49272(B) TYPE: NUCLEIC ACID(C) STRANDEDNESS: DOUBLE(D) TOPOLOGY: LINEAR(ii) MOLECULE TYPE:(A) DESCRIPTION: OLIGONUCLEOTIDE(iii) HYPOTHETICAL: NO(iv) ANTI-SENSE: NO(vi) ORIGINAL SOURCE:(A) ORGANISM: MYCOBACTERIOPHAGE(B) INDIVIDUAL ISOLATE: D29(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1:GGTCGGTTATGCGGCCGAGCCATCCTGTACGGGTTTCCAAGCCGATCAGAAGCACGGGCC60GACGCTAGAGCGCCTCGCCCAGGCGCTGTGAGCCACCAGGAGCCACGAACTCGCGACCCA120CGGGGAGTTATACCCCCGGAATCGGCTACGGGCCATACAGGCCCGGTATCTGTCAAGGTG180ATCTACGCCACTCCGTGGGGTGGCTGTCAAACGCAGACTCCTTCCCTATTAATGAGGGGC240TGAAGGCCCCTCCTAAGAGCCGCTTCAGGCGGCTCGCTAAGAGCGCCTTTCGGGCGCTCC300TGAGTAATAACCGGCCCAACAAGGGCCGGGGAATTGATCCGGCAACCGCCGGATCTAGCG360CCGCGCCTGAAGCGCGGCTTATTGAAGGGGTGACGCAACCGTGTACGGCACTCGTTCGAG420TGCGTACTGGAGCACTCAACCGGGGAAGTTCGACGTTCTCAACCTTCGGATGACGTTCCC480AAGCTCATCCGCGTACGAGATCCCCGATCTGCGGCCGACAACCTACGTTCCGGCCAACCT540CGCGGCCTGGAACATGCCGCGACATCGCGAATACGCCGCCGTTTCGGGCGGCGCACTGCA600CTTCTTCCTTGACGACTACCGATTCGAGACCGTCTGGTCGAGCCCGGAGCGCCTTCTCCC660CCGCGTGCAAGCGGTTGGAGCTGCTCTGACGCCCGATTTCAGCCTCTGGCGGGACATGCC720ACGGGCCGCAGCGGTCTGGAACGTCTACCGCAGCCGCTGGTGCGGCGCGTACTGGCAGTC780GCAAGGGATCGAGGTGCTCCCAACCGCGTGCTGGGCGACCCCAGACACGTTCGACTTCTG840CTTCGACGGGATCCCGGAGGGGGCCACGGTCGCGATCAGCTCGATGGGCATCCGCTCCTC900GAAGGTCGACCAGGCGCTTTTCCGCGCTGGCCTCCAGGAACTCCTCGATCGCAAGCAACC960GCGACTGCTGCTGGCCTACGGCCGGCTGCGGTACTGCGACGACATGAACCTTCCCGAGGT1020CAAGGAGTACCCGACCTACTGGGACAGACGACGAAAGCAGGTATCCGACGCATGGGCGGA1080AGAGGATCCGCCGGTAGCGGCGGTGCCCCTGGAACCCGAGGACGCAACAGCACCCGGCAA1140GGAGCCGGGGCAGGCGGCGTGGGTGGACTTGGAGGCGGCGGTGGAGCCGCCGGAAGCGGC1200CGGGGCGGTTTAGGCACCGGCAACGCAGGCACCGGTGGTGTACTCGGTGGCGGCGGCAGC1260GGAGCTGGCGGCGGGTTCGGTGGTTCGACCGGCAGCCAGACGCCAGACTGGACCTACAAC1320AGCCCGATGGACCCCGCGCAGAAGCGTGCGGCGTTCAACGCGCTGGCGGTCGCCGCCCGC1380GACCAGCACTCACCGTCGGATGCCAAGCGCATAGCGAAGAGGGACCAGATGCTCGGATAC1440GTTCGAGGGCCGTGGGAGCAGTTGGAGGACGACGACTCCACCCGCTACGACACCCTGAAG1500CAGGCGATGGACGACGCCATGTCCAAGATCCTGAGCCAGGCGCAGGTGGTCCACCGCACG1560AAGCACCTGGACAAGCTCCTGGACTCGGGCCGGATCTCCTCGCTGTTCGAGGTCGGCTTC1620TCGGCCGGTGGCGACACCCCCGGTCAGCGCGCTCTGTACGAGGAGGCGTGGTTCGGCAAG1680GGCCACGTCCCGCCGGTCTACTCCGCGCTGGAGTTCGACGGCGTCAAGCCCAAGGGCATG1740AGCATGTACGGCAGCACCAAGCTCTACCTCAAGCCCGAGGTGCGTGACCGGGTCACTGTG1800ACCATCGGCGACTCGCTGATGTCGAGCGACAGCGTGTTCCCCGGAAAGCCGGGTGACGGC1860CTCGGCCTGCGGGCGAATCCGAATGCGATCAAGAACCTCGTAGACCCGAATAAGTCTCGC1920GAAGAGAACATGCAGGCGATCTACGAGAGCTTCAAGAAATACGCCGAATCGAATTTCATC1980GAATCGCAAATTCACGACGGCGTTATTCTCGAAGACATCGAGAAGGTTGTATTCACTCAG2040CCGCCCAGCTCTTTCCTGACTGATAAACTGGACAAGCTCGGAATACCGTGGGAGGTGGAA2100TCGTAATGGCGCTGATGCAAGCGACACACACCATCGAGGGCTTTCTCGCGGTCGAAACGC2160ACCCGAGGGCGTTCGTGGCCGAGAATGGCCACGTGATCACCCGCCTCTCGGCCACCAAGT2220GGGGTGGCTGGGAGGGCCTGGAGATCCTTGAGTACTCGGGTGACGGCCAGGTCGAGGTCA2280GCGACGAGCAGTTGGCTGAGGCGGAACACGCCAGCCAGATCGAAGCACAGATCATCGCGG2340AGGCAGCCGCAGAGTGAGTTGGGCCGGCTCGAAGCGTCGGCAAGAACTACCGGAGGACTG2400GGAGCTGAATTACCGGCTCCCGGTCCTTTCTGCTGCCGGGTGGCTGTGTGAGGTCGACGG2460CCCCGGCTGCGTCCGGGCTGCCACCGACGTAGACCACAAGAAGCCAGGGAACGACCACTC2520GCGGTCTAACCTGCAAGCGATCTGCCGTGTCTGCCACGGCAAGAAGTCAGCCGCTGAGGG2580CGTAGCCCGACGGCGGGAACTCAAGGCCCGGAGGAAGCGACCAGAACAACGCCACCCTGG2640GCGTCGTTAAGCGGGCCAGGTGCCTGCTCCACCCAGGAGGTGACTGTGGGCACACGTGGC2700CCCATCGGAAAGCGCGACGAAGAACGCGTTCGTCGCAACACCCCGGAGAACCCGACCGAA2760ACGATCTCGATGATCGGGACGGTGGAGATCCCGGAACTCGGCGACATGAGCTACATGGGC2820GAGACCCATCCGCTCATCGAAGAGATGTACGACGCGATCAAGCAATCGGCAGCCGTGAAG2880TTCTACGAACCGACCGACTGGCAGTTCGCTCGCCTCGCTCTCTACACACTGAACCAAGAA2940CTCATCGCGGCCAAGCACCAGGGCAAGCCCATCGGTGCGATGAAGCTGACCGCGATCAAC3000CAGATGCTCTCCGCACTGCTGCTGACCGAAGGCGACCGGCGACGGGTCCGCCTGGAGATC3060GAGCGGGCACCCGCCGACCCGACCGGCGGGAAGGTCGTTGACGTGACCGACGTGCTCAAG3120CAGCGCCTCGCCAAGGCGAGCGGCGGGGGGTGATGGTCCCCCTGGCGGGGTTCTGAGCGG3180TTGCCGCTACCGACTGCTCCCCCGCCGGGGGTTGACCCTCCGAACTTGAAAGGATCCGCA3240TGGCCGACCTCGGCAACCCGTTCGACCTGGAGATGCTCTGCCTGGTCACCGGCCGCGACT3300TCCGCTGGTCGATCCCCCACCTCGACCCGGTCACCAAGCAGCCCACCCCCTGGCCTGCGG3360GCGACCTGTTCCTCGAACTGGAGACCGGCGGCGAGCACAACGCGCTGCACCAGGTGTACA3420TCACCGGGGCCACAGGTGGCACGTACACCCTGAACCTCAACGGCACCGACACCCCGGCCA3480TCGACTACAACGACGTGTCCGAGAACCCGCAGGGTCTGGCCGGTGACATCCAGGACGCCA3540TCGACGCGGCGCTCGGGGCCGGTAACGGCCTGGTGCATCCGGTCTCGCTGTTCCCCGCGT3600GGACGCTGAACTTCAACCTGAACGCCCGCAAGCCGCTGACCGAGCAGTTGGTCAACACGA3660TCAACAAGGCCACGAACGACTTCTTCGATGCGTTCGACCAGCTCCTCGGGGTCGACGTGG3720AGATGACGGTCACCGACACCCTGAACTTCCAGCTCAAGGTGACCTCGCGGCGCTCGTTCG3780ATGAGGTCGGCGTCGTCACCTTCGCGGTCGGCGTGACCTCGACGGCGGTCAAGAACTTCT3840TCAACGGCTTCTCCGGGCTGATCGGCGCGGTGAACACCGTCAACGTCGACTTCTACTGGA3900ACCGGACCTACGACATCGAGTTCGTCGGCGAGCTGGCCGAGACGCCGGTACCCGCCTCCA3960CGGCGAACGCGGCCGGTCTCACGGGCACCTCGAAGGCCATCACCGTCTCGGTGGTCGAGC4020CAGGCAAGGACCGCCTGACCATCTGGCCGTTCACGATCGACGGCGTGACCGCCTCGATCA4080AGGTCGAGTCCGAAGAGGCCGACAAGATCCCGAACCGCTGCCGCTGGCAGTTGGTTCATC4140TGCCGACCGGCGAAGCGGCCGGCGGTGACCCCAAACAACTCGGCGTCGTCTACCGGCAAC4200CCCGGTAGGCGCACCACTGACGTGTAGCTCAATGGCAGAGCATCCGGCTGTTAACCGGAC4260GGTTGAAGGTTCGAGTCCTTCCTCGTCAGCCAAGCGGGCGGTTCCCCAGAGCGTGGGGAG4320CCCCCGCACCAAGTACACGTAGCTCAATTGGTAGAGCAGCGGTCTCCAAAGCCGCCGGTT4380CCAGGTTCGACTCCTGGCGTGTATGCCACCACCCTTCCCCGTTCGTCTAATCGGTAAGAC4440GCCTGGCTCTGGACCAGGTAATTGAGGTTCGAGTCCTTGGCGGGGAGCACCCACCCAGTT4500CCCTTGTGGGGCTGGGTCTTTCGGTCCCTTGGAGTAGCGGATAACTCACCTGGCCCTCAC4560CCAGAAGATCGCGGGTTCGAATCCCGCAGGGACTACAAACGCGAGATACCCAAGCGGCAA4620CGGGATCTGACTGTAAATCAGACGCTTCGGCTTCGCAGGTTCGAGTCCTGCTCTCGCGAC4680TTGACAGCCACCACGAAAGGAACCCATGACGCTCATAGTCACACGCGACCACGCGCAGTG4740GGTCCACGACATGTGCCGCGCTCGCGCTGGCAACAGGTACGGCTACGGCGGGGCGTTCAC4800ACTCAACCCCCGAGACACCACCGACTGCTCGGGTCTGGTTCTGCAGACGGCAGCCTGGTA4860CGGCGGTCGGAAGGACTGGATCGGAAACCGGTACGGCTCGACTGAGAGCTTCCGGCTCGA4920CCACAAGATCGTCTACGACCTCGGGTTCAGGCGACTCCCTCCGGGAGGCGTTGCGGCCCT4980GGGATTCACCCCGGTCATGCTCGTCGGGCTCCAGCACGGCGGCGGGGGCCGGTACTCGCA5040CACCGCTTGCACGCTGATGACGATGGACATCCCCGGTGGCCCGGTGAAGGTCTCGCAACG5100AGGCGTCGACTGGGAGTCCCGAGGAGAAGTCAACGGCGTGGGGGTGTTCCTCTACGACGG5160CGCACGCGCCTGGAACGACCCGCTCTTCCACGACTTCTGGTACCTGGACGCGAAGCTTGA5220AGACGGCCCGACGCAGAGTGTCGACGCTGCCGAAATCCTCGCTCGCGCAACGGGTCTCGC5280GTACAACCGAGCGGTAGCACTGCTGCCGGCCGTGCGTGACGGCCTCATCCAGGCCGACTG5340CACCAACCCGAATCGCATCGCGATGTGGCTCGCCCAGATCGGCCATGAGTCAGACGATTT5400CAAGGCCACTGCGGAGTACGCCAGCGGGGACGCCTACGACACCCGAACCGACCTCGGCAA5460CACCCCGGAGGTCGACGGAGACGGTCGGCTCTACAAGGGCCGGTCCTGGATCATGATCAC5520GGGCAAGGACAACTACCGGGACTTCTCCCGGTGGGCTCACGGCAGGGGCCTGGTCCCCAC5580GCCCGACTACTTCGTGGTTCACCCGCTGGAGCTGTCGGAGCTGCGCTGGGCAGGCATCGG5640TGCCGCCTGGTACTGGACCGTCGAGCGCCCAGACATCAACGCACTCAGCGACCGCCGCGA5700CCTCGAAACGGTCACGCGCCGGATCAACGGCGGGCTCACCAACCTCGATGACCGCCGACG5760CCGGTACAACCTGGCCCTCGCTGTGGGCGACCAACTACTGACTCTGATCGGAGATGACGA5820CGAATTGGCTGATCCAACGATTCAGCGGTTCATCCGCGAGATCCACGGGGCGCTGTTCAA5880CACCGTCGTGACGCAGTCCCCCTACGGCGACCCGCAGAACCCGGACGGCTCGGAGCCCCG5940GAGCAACCTCTGGCAGCTCCATGAGCTGATCAAGAACGGCGACGGCATGGGGCACGCCCG6000CTACGTCGAGGAATCGGCGCGAGCCGGTGACCTCCGCGAGCTGGAGCGAGTTGTCCGCGC6060CGCCAAGGGACTTGGTAGGGATCGCTCCCCCGAGTTCATCGCACGCGCTCGGAACGTGCT6120GGCCCAGATCGAGGCAGCCAACCCCGAGTACCTACAGGCGTACATCGCCAGGAATGGAGC6180CCTATGAGCCCCAAGATCCGTGAAACGCTCTACTACGTCGGCACTCTCGTCCCCGGCATC6240CTGGGCATCGCCCTGATCTGGGGCGGGATCGACGCGGGCGCAGCCGCGAACATCGGCGAC6300ATCGTCGCTGGCGCTCTCAACCTGGTCGGCGCAGCCGCACCGGCCACGGCCGCTGTCAAG6360GTCAACCAGCAGCGCAAGGATGGCACGCTGACCACCTCCCCGGTGGATCAGGTCACCAGG6420GGCGTCGAGCAGGTGCTCGCGGCCAAGCAGAACGCTGAGGCTGAGGTCGAGCGCGTCAAG6480CAGGCTCTGGAGTCCGCTGTCAACGGCGCGGTCCCCCAGCTCGGCCCGCTGGCCAGCCAG6540ATCCTCAACGGCATCCAACCGGCCTACAGCCAGCCGTTCGACCCGCACACGCAGCCCTGG6600AACCGATGAGCAAGCCCTGGCTGTTCACCGTTCACGGCACGGGCCAGCCCGATCCCCTCG6660GGCCTGGCCTGCCTGCCGATACGGCACGCGACGTACTTGACATCTACCGGTGGCAGCCCA6720TCGGCAACTACCCCGCTGCGGCCTTCCCGATGTGGCCGTCGGTCGAGAAGGGTGTCGCCG6780AGCTGATCCTGCAGATCGAGCTGAAGCTGGACGCGGACCCCTACGCGGACTTCGCGATGG6840CGGGTTACTCGCAGGGAGCCATCGTGGTTGGCCAGGTGCTCAAGCACCACATCCTGCCTC6900CGACGGGCAGGCTCCACAGGTTCCTGCACCGGCTCAAGAAGGTCATCTTCTGGGGTAATC6960CCATGCGGCAGAAGGGCTTTGCCCACTCTGACGAGTGGATCCACCCGGTCGCTGCCCCTG7020ACACCCTCGGAATCCTCGAGGACCGGCTCGAAAACCTGGAGCAGTACGGCTTCGAGGTCC7080GCGACTACGCCCACGACGGTGACATGTACGCCTCCATCAAAGAGGACGACCTGCACGAAT7140ACGAGGTCGCCATCGGCCGGATCGTGATGAAGGCCAGCGGCTTCATCGGTGGCCGGGACT7200CCGTGGTAGCCCAGCTCATCGAGCTTGGCCAGCGTCCGATCACCGAGGGAATTGCGTTGG7260CGGGAGCCATCATCGACGCCCTCACGTTCTTCGCCCGCTCTCGTATGGGCGACAAGTGGC7320CGCACCTCTACAACCGCTACCCGGCGGTCGAGTTCCTACGACAGATCTGAGAAAGGAGGC7380GGGGTGAGCCTCGACAATCACCACCCGGAGCTTGCCCCGTCTCCGCCTCACATTATCGGC7440CCGTCATGGCAGAAGACGGTCGACGGAGATTGGCATCTGCCGGATCCCAAGATGACCCTT7500GGATGGGGCGTCTTGAAATGGCTGTCGGAGTACGTCAATACCCCTGGGGGACATGACGAT7560CCGAACCGCCTCAAGGTTTTGATCTCGCTGTCCGAAGCAGGACTGCTTGAGAACGAGAAC7620ATGTTCATCCCCACCGACGAGCAGGTACGCCTGGTCCTCTGGTGGTACGCCGTAGACGAG7680AAGGGCCAGTACGTCTACCGCGAAGGCGTGATCCGGCGGCTCAAGGGATGGGGCAAAGAC7740CCGTTCACCGCCGCGCTGTGTCTCGCCGAACTCTGTGGCCCAGTAGCATTCTCACACTTC7800GATGAGACCGGTCAGGCGATCGGCAAGCCGCGCCCCGCAGCGTGGATCACCGTCGCGGCC7860GTCAGCCAGGACCAGACGAAGAACACGTTCTCGCTGTTCCCGGTGATGATCAGCAAGAAG7920CTGAAGACCGAGTACGGACTCGATGTCAACCGGTTCATCATCTACTCCGCGGCCGGTGGC7980CGCATCGAGGCAGCAACCTCCTCACCAGCATCGATGGAGGGTAACCGCCCGACGTTCGTC8040GTTCAGAACGAGACCCAGTGGTGGGGCCAGGGGCCTGACGGCAAGGCCAACGAAGGCCAC8100GCGATGGCCGAGGTCATCGAAGGCAACATGACCAAGGTCGAGGGCTCTCGCACCCTGTCG8160ATCTGCAACGCCCACATCCCCGGCACCGAGACGGTCGCCGAGAAGGCGTACGTCGAGTGG8220CAGGACGTGCAGTCCGGGAAGTCCGTCGACACAGGCATGATGTACGACGCTCTCGAAGCG8280CCGGCCGACACCCCGATCTCCGAGATCCCTTCTGAGAAGGAGAATCCCGACGGGTTCCGG8340GAGGGCATCGAGAAGCTCCGCGAGGGGCTGTTGATCGCCCGAGGCGACTCCACGTGGCTG8400CCGATTGACGACATCATCAAGTCGATCCTGTCGACCAAGAACTCGATCACCGAGTCACGG8460CGCAAGTTCCTCAACCAGGTCAACGCGGCCGAGGACTCCTGGCTGTCACCGCAGGAGTGG8520AACCGCTGCTTCGCCGACCCGGACAAGTACCTCGACAAGATGGGCTTCGAGCTGGCTCCG8580CTGGACCGGGGCCAGAAGATCACCCTCGGGTTCGACGGGTCCAAGTCCAACGACTGGACG8640GCTCTGGTCGGCTGCCGGGTCTCCGACGGCCTGCTGTTCGTCATCGACATCTGGGATCCC8700CAGAAGTACGGCGGCGAAGTGCCCCGCGAGTTCGTGGACGCTGCAGTGCATTCCGCGTTC8760TCCCGGTACGACGTGGTCGCGTTCCGCGCCGACGTGAAGGAGTTCGAGGCATACGTCGAC8820TCCTGGGGCCGGACCTACAAGAAGAAGCTCAAGGTCAACGCCAGCCCGAACAACCCGGTG8880GCGTTCGACATGCGCGGTCAGCAGAAGCGATTCGCGTTCGACTGTGAGCGCCTGGAGGAC8940GCGGTCCTCGAAGGCGAGGTCTGGCACGACGGCAACCCGGTGCTTCGGCAGCACGTCCTG9000AACGCCAAACGACACCCAACTACCTACGACGCCATCGCGATTCGCAAGGTCACCAAGGAC9060TCCAGCAAGAAGATCGACGCTGCAGTCTGCGCTGTCCTCGCGTTCGGGGCGAGACAGGAC9120TACCTCATGAGCAAGAAGGCCCGCACGGGCCGGGTGGTGGCCGTCCGATGACAGCCCCGC9180TCCCCGGACAGGAGGAGATCGCTGATCCGGCGATCGCCCGAGACGAGATGGTCTCGGCGT9240TCGAGGACCAGAACCAGAACCTCCGATCGAACACCAGCTACTACGAGGCTGAGCGCCGAC9300CAGAGGCCATCGGCGTCACGGTGCCCGTCCAGATGCAGTCACTGCTGGCTCACGTCGGAT9360ACCCCCGGCTGTACGTCGACTCGATCGCAGAGCGACAGGCTGTCGAGGGTTTCCGGCTCG9420GGGACGCCGACGAGGCGGACGAAGAGCTGTGGCAGTGGTGGCAGGCGAACAACCTCGACA9480TCGAGGCCCCGCTGGGCTACACCGACGCTTACGTCCACGGCCGGTCGTACATCACGATCA9540GCCGCCCTGACCCCCAGATCGACCTTGGGTGGGATCCGAACGTCCCGCTGATCCGGGTGG9600AGCCCCCGACGCGCATGTACGCCGAGATCGATCCTCGGATCGGTCGGCCGGCCAAGGCAA9660TTCGTGTCGCGTACGACGCAGAGGGCAACGAGATCCAGGCTGCCACGCTCTACACCCCCA9720ACGAGACGTTCGGGTGGTTCCGGGCCGAAGGCGAGTGGGTGGAGTGGTTTAGCGACCCCC9780ACGGGCTCGGCGCGGTCCCGGTGGTCCCGCTTCCGAACCGGACCCGGCTCTCGGACCTGT9840ACGGCACCTCTGAGATCACCCCGGAGCTTCGGTCGATGACCGACGCGGCGGCTCGAATCC9900TGATGCTGATGCAGGCGACTGCGGAGCTGATGGGCGTGCCCCAGCGACTGATCTTCGGCA9960TCAAGCCCGAAGAGATCGGCGTAGACCCCGAGACCGGACAGACGCTGTTCGACGCGTACC10020TCGCCCGCATCCTGGCGTTCGAGGACGCCGAAGGCAAGATCCAGCAGTTCTCGGCAGCCG10080AGCTGGCCAACTTCACCAACGCACTCGATCAGATCGCCAAACAGGTCGCTGCGTACACGG10140GACTCCCTCCCCAGTACCTGAGTACCGCCGCTGACAATCCGGCCTCCGCTGAGGCCATCA10200GGGCCGCAGAGAGCCGCCTGATCAAGAAGGTCGAGCGGAAGAACGCGATCTTCGGCGGTG10260CGTGGGAAGAGGCGATGCGCCTGGCCTACCGGCTGATGAAGGGTGGCGACGTTCCCCCGG10320ACATGCTCCGCATGGAGACGGTCTGGCGTGACCCGAGCACCCCGACGTACGCCGCGAAGG10380CTGACGCCGCCACGAAGCTCTATGGCAACGGCCAGGGCGTGATCCCGCGTGAGCGGGCTC10440GCAAGGACATGGGCTACTCCATCGCCGAGCGCGAGGAGATGCGCCGCTGGGACGAGGAAG10500AGGCCGCGATGGGCCTCGGCCTGCTGGGCACGATGGTCGACGCCGACCCGACGGTCCCAG10560GCTCCCCCAACCCCACGCCAGCTCCCAAGCCACAACCGGCCATCGAAGGGGGTGATTCCG10620CCTGACGCCTGAGCAGTATGCAGCGGCCCAGGCCGTGATCACTGCGGGGCTCGCCGGGTA10680CGTCCAGCGGTTCGCCAGTCTCTTCATCCGCCCAGCTCTCTCCATCGCGGAGTGGCTGCG10740GCTACTGCAGGTGTTGTTCCCAGAGGTCCAGCGTCGGTATGCGGAAGCTGCCGACCTGGG10800CCGGGACTTCTACGACTCCCAGCGCAGACTCCACCACCCGGAGCTTCCCCGCAACGAGAG10860GTTGCGGAGCGACCTCCAGTGGGAGTGGTTCGTCAGGAACATGGAGCCCGCACGAAAGGG10920GTTGTCGCAGGCCGACTCTCCTCAAGCTGCGGTCACCAAGCTGACCTTGGCGACAGTGCG10980CGAAGTGGAGATGGCAGGTCGCCGACAGATCATCGGCGCTGTCAAGAACGACCCAGCTCC11040TAAGATCGTGAAGGGCTGGGCGAGGGTCGCCACCGGGCGCGAAACATGCGCCTGGTGTCT11100GATGCTGATTTCCCGTGGCCCCGAGTACCTTTCGGCGGATAGCGGGGGTCTTCACCTCGA11160CACCGAGACCGTGGTCGACCTCTGGAACGAGGCTGGCCGCGATCTGGAGAAGTTCCGCGA11220AGAGACCAAGCCCCACATCGAGGAGTGGCACGCAGGGTGCGACTGCCTGGTGGTGCCTGT11280CTTCGACGTGGAGAACTGGCCCGGAAAGGCCGCACAGGAACGCGCTCTGCAGCTCTGGAT11340CGACGCTGGGAAAGAAGCCAGCCAGCTCATTGCATCTGGCAAGGCCCGCTCCAAAAACGA11400GAACAAGGAGACGATCAACGCTCTCCGTCGCCGCTTGTATCGCGGCGAGTTCGCAATGTC11460CGACTACGCACTCGCTGCGTAATCCCCCGAACCCCAGGTGGGTTCATCAACCATGCCCAG11520GAGGCGAAAACACATGTCCGACACCGCAACTACCGAAGGCACTCCGGCCGGCGACCCGAC11580CCCGGTGGTCACTGACAAGCCGCTGGAACCGACTCCGAAGACCTACGACGAGGCATACGT11640CAAGGAGCTTCGCCAGGAGGCCGCTGCTGCTCGGGTTGCCAAGAAAGACGCAGTCGAAGC11700CGCAGTCAAGGCGGCGAACGACGCTCACACCGCTGAACTCGCTGCTCGCGACACTCGAAT11760CACCGAGCTGGAGAACGAGCTGGGACAGGCTTGGATCCGGCTGCAGAAGCTGGAGACCTC11820GCTTGCCGCAAAGGTTCCCAGCGACAAAGTGCTCGCGTTCGTTGACATCTTGCAGGGCGA11880GGACGCCGACTCGATCGCCGAGTCCGCGAAGAAGAACCTCGAACTGATCGGCGGTTTCGA11940CAAGAAGCCGGTTTCCGGTTTCGACCCCACCCAGGGCTTCGGTGGTCGGCAGGAACTGCC12000GCTCAACGGTGACCCGATCCTCAACGCCATGAAGGGCGTTCTCGGCATCAAGTGATGTCG12060AGCTGAAACCCATTCCTAGACAAGGAGATTAGCAGATGGCCGCAGGCACTGCTTTCGCAG12120TTGATCACGCTCAGATCGCCCAGACCGGCGATACCATGTTCAAGGGCTACCTGGAGCCCG12180AGCAGGCGAAGGACTACTTCGCCGAGGCCGAGAAGACCTCGATCGTCCAGCAGTTCGCCC12240AGAAGGTGCCGATGGGTACCACGGGCCAGAAGATCCCGCACTGGGTCGGCGACGTGAGTG12300CCCAGTGGATCGGTGAGGGTGACATGAAGCCCATCACCAAGGGCAACATGACTTCGCAGA12360CCATCGCGCCCCACAAGATCGCGACGATCTTCGTGGCGTCTGCGGAGACCGTCCGTGCCA12420ACCCCGCCAACTACCTGGGAACCATGCGTACCAAGGTGGCGACCGCCTTCGCGATGGCGT12480TCGACGGCGCGGCGATGCACGGCACCGACAGCCCGTTCCCGACCTACATCGGTCAGACCA12540CCAAGGCCATCTCGATTGCTGACACCACCGGTGCCACGACCGTGTACGACCAGGTGGCCG12600TGAACGGCCTGAGCCTGCTGGTGAACGACGGCAAGAAGTGGACCCACACCCTTCTGGACG12660ACATCACCGAGCCGATCCTGAACGGCGCGAAGGACCAGAACGGTCGCCCGCTGTTCATCG12720AGTCGACCTACGGTGAGGCCGCGAGCCCGTTCCGTTCGGGCCGGATCGTCGCCCGTCCGA12780CCATCCTCAGCGACCACGTCGTGGAGGGCACCACGGTCGGCTTCATGGGTGACTTCTCCC12840AGCTCATCTGGGGCCAGATCGGCGGTCTGTCCTTCGACGTGACGGATCAGGCGACCCTGA12900ACCTGGGCACCGTCGAGAGCCCGAACTTCGTCTCGCTGTGGCAGCACAACCTCGTCGCAG12960TCCGTGTCGAGGCTGAGTACGCGTTCCACTGCAACGACGCCGAGGCGTTCGTCGCTCTGA13020CCAACGTGGTCAGCGGCGGCGGCGAGGGCTGAGCCTAACTTGACATCCACCGGGAGGGGG13080CCGTTCACGCGGCCCCTTCCTGGGGTGTCTGAGAGGACTTCATGCGGATCCAATCCACCG13140TCAACGGTGGGTTCGCGGAGGTCTCCGACGAGTACGCCCAGCGCCTGATCGCGGCTGGCG13200GTTGGAAGCGTCCTCGGAAGCCTCGCACCACCAAACCCAAACCCGCTCCGAAGCAGGAGC13260CTGCGACCGAGGAGTAACACATGGCCTACGCGACCGCTGACGACGTTGTGACGTTGTGGG13320CCAAGGAGCCTGAGCCAGAAGTCATGGCGCTGATCGAGCGCCGACTCGAACAGGTCGAGC13380GCATGATCCGGCGTCGGATCCCAGATCTGGACGCCAGGGTGTCTTCGGACATCTTCCGGG13440CCGATCTGATCGACATCGAGGCCGACGCGGTGCTGCGTCTGGTGCGTAACCCGGAGGGCT13500ACCTCTCGGAGACCGACGGGGCGTACACCTACCAGCTCCAGGCTGATCTGTCTCAGGGCA13560AGCTCGTCATCCTTGACGAAGAGTGGACGACCTTGGGAGTCAACCGACTCTCGCGCATGT13620CCACCCTCGTCCCGAACATCGTGATGCCGACATGAGCGCCAGCGACGTTCAGCGGGCTCC13680GATCAAGTACCCGCCGGGGTTTCTCCTGGCGGTCACACCTGACCAAGTCGACGCCGCGAT13740GTGCGACCACGAAGCGGATCCTCCGGTCTGCTACTGCGTCCACGACTGGCGCATCGAGTT13800CGGCAACGTCTCTCGCCAGCCCAAGCCGAAAGCGACGTACATCTGATGAGCCTCCTCGAT13860ACCGGAGCCCGGTACCAGCCGGTGCTCGTCTACCCCGAAGAGCTGGTCATCGACGCGGAC13920GGGAACAAGAAGACCCAGCCGTCGAAGACCCCGATCCAAGCGATCGCACGCTTCCAGGTC13980GCCAACCAGTCCGGTACGTCGGCACGACGTGCTGAGCAGGACAACGGGGGGTTCACGACC14040GAGAAGGTCTACCGGATGCGGTTCCCTCGCTCCTTCACCAAGGAGCACGGGATCCTCGGC14100GCTCAGACCCAGATCGAGTGGAAGGGCCAGCGGTGGGCGCTCTTTGGAGACGCCACCGAG14160TACGACTCATCGCCCGCACTGGCGCGGGTCGACTACACGATCAAGAGGTTCTGATGGCGA14220AGGTCTACGCGAACGCGAACAAGGTCGCGGCCAGGCACGTCGATGTCCGCAAGCGGGTCA14280AGGAGGAGCGAGACGGCGTCACCCGCCGTGCTCGAACCAACCTGGCCAGAGCGAACAAGA14340CGACCCGTATCACCAAAGAGGGGTACTTCCCGGCATCGATCGAAGAGGTCGACGGCGATG14400TGGACTTCCACACGGTCCTGCACGCGCCCAACGCGTTCGCCCTTGAGTTCGGCCACGCCC14460CGTCCGGGTTCTTCGCGGGCACCGACACGAAACCGCCTGACCCCGAATACATCCTGACCC14520GAGCCGCCATCGGCGGCACCGTCTCGTAAGGAGGACACATGGGGGCGATGCCCCGAGTAC14580AAAGTGTGGTTGCTCCGATCCTCCGAGAAGACCCTCGACTCGCCGGGGTCACGATTGTGA14640CCTGGGTTCCCGACATCGACTTCCGTGAGTTCCCGATGATCAACATCCGCCGCATCGGCG14700GGATCAGGAACGCCAACGCCCCGAAGCTGCACTCGCTGCCGGTGGTCGAGATGTCGGCGT14760ACTCCACTGACGGGCTCATCGAATGCGAGGAGCTGTACGAGACAGCACTTGAGGTGCTGT14820ACGACGCGGTGAAGAACGGAACACAAACTCCCGCAGGGTATTTGAGTTCGATCTTCGAAA14880CGATGGGCGCCACCCAGTTCAGCTCCCTCTACCAGGACTCCTGGCGAATCCAGGGCCTGA14940TCAGGCTCGGCGTCCGCACACCGAGATCCACCACCTAACCGAAAGGTAGCCACATGGCAG15000AAAACGACGATGCAGTTTTGACTGCTGCGGTCGGGTATGTGTACGTCGCCGAAGCAGGCA15060CCGCTGCACATACGCCGGCCGAACTGAAGACCATCGACCTGTCCGACCCGTCGACCTGGA15120CTGGAGCCACCGGCTGGTCGAGCGTCGGCCACACCAGCCGAGGCACGCTCCCCGAGTTCG15180GCTTCGAGGGCGGCGATTCCGAGGTCAAGGGCTCCTGGCAGAAGAAGAAGCTCCGCGAGA15240TCACCACCGAGGATCCGATCGACTACGTCGTGGTCCTGCTGCACCAGTTCGATGAGCAGT15300CGCTCGGCCTGTACTACGGCCCGAACGCCTCCACGACCCCCGGTGTGTTCGGTGTGAAGA15360CCGGCCAGACCAACGAGAAGGCGGTCCTGGTCGTGATCGAAGACGGCGACATGCGCCTTG15420GCCACCACGCCCACAAGGCCGGTGTCCGTCGCGACGACGCGATCGAGCTGCCGATCGATG15480ACCTTGCCGCACTGCCGGTTCGCTTCACCTACCTCGACCACAAAGACGAGCTTCCGTTCT15540CGTGGATCAACGAGGATCTGTTCGGCCTCTCGCCGGGCGGTGGAGCCTGACCCAAACTTG15600ACAGCCACCAGGCTGTCTACCCCGGAGGGGGAGGTTTCCTTGGCGGGCCTTGCCTCCCCC15660TCCTCCCGCCATCTAGCCCGCCACACACTCTGAAAGGTTCGCCATGACAAACGTATTCAC15720GATCGACGCATTCCGCGAAGAAGTCAAGAAGAAGTACGAGCCCGTCACGATCGGGATCTC15780CGAAGATGTGACCGTCGAGCTGAAGCCGCTGCTGAAGCTGGGCCAGAAGGCCCGCGAAGC15840CGTGGTCGAGGCCGTCAAGGAGGTCGAGGACATCCCCGACATTGACGAGGACGACGAGGA15900GGCCGAGGAGCTGGTGGACGAGTACTCGCTCCGCATTTGCGAGATCGTCGCCAAGGTCTT15960CCGGCTGATCGCCACGAAGCCCAAGAAGCTGATCGCCGCGCTGGACGAGGAAGAGGATCC16020CAGGATCCGGGCCGAGCTGTACGCGACCGTACTGCGGACCTGGATGGTGGAGACGCAACT16080GGGGGAAGCCGCGCCCTCGCCGAGCTGATCGACAAGTTCGGCGGGGCGATTCTCTCCGAC16140TTGTCCGAGTACCACGGGGTCGACCTGCGCGATCTGTTCAGAGATGAAGATCCGCTGTCC16200CCCAGGTACGTCCTGAATCTGGTGATTCACCTCCCGAAGACAGGCGCGTTCTACGCGGAG16260CGTCGAGGTGGTCAGCAGTACCGAGGTTGGGATGAGGACCGGTACGCGCTCGCGGACATC16320TACGACGCAGTCCAGGCGGGCAACCACATCCTCCTGATGGCCAACCGGGATCCGAAGAAG16380CCAAAGCCGAAGGCACCCAAGGCATACCCGCGTCCCGACGACTTTGAGAAGACAACGCCG16440AAGCCAGGTTCGTTCGCCGCGATGGTAGTGGCCGCGAAGAAGGCTGCGCGAGAGAAGAGG16500GAAAGGGAGGAGGCGAATGCCGAATAGTGCTGGCGTAGAGGTCGCACGGATCTCCGTCAA16560GGTCAGCCCCAACACCAAAGAGTTCCGTCGTGAGCTGAAGACCGATCTGGAGAAGATCGA16620GCGGGAGCTGTCGGCCGACGTTCCGGTCAACGCCGACCTGAACGCGGCCCAGGCCAAGGC16680CGACTTCAAGCGGCTGATGATGCAGCTCAAGACCGAGGCTGCCCGAGGTGTCAACATCCC16740CGTCGATGTCAACGTCGACAAGGACACCAAGGGCGGCTTCCTGAGCCGCCTGCTCGGCGG16800TAAGAAGGGACTGAGCAGTCTTGGTGATGACGCCGCGAAGGCGTCGTCTCAGGTTCAGCA16860CCTGGGCAAGTCGTTCCTGGATCTGACCCGCACAGCGTGGATCGGCGTCGGGATCGTCGC16920CATCGCCGCGCCGCTTGTCGGCTTGGTGGCTGGCCTACTGGCTGGCCTGCCGTCGCTGCT16980GTCAGCGTTCGGCGCTGGAGCCGGTGTGGTCGCACTCGGCATGGACGGCATCAAGGCGGC17040AGCCGAGACCATGATGCCCGCGCTGGAAGCGGCCAAGACCGCCGTCTCCTCGACGTTCCA17100GACTGGCCTCACCCCGGTCTTCCAGCAGCTCGGTGGGTTGCTGACGACCCTCACCCCGAA17160CTTGCAGAACGTCGCCACCGGCATAGTGAACATCGCCAAGGGCTTCACCGACGTGGTGTC17220GCAGGGTCCGGGGCTCCAGCAGCTCCAGAACATCCTCGACCGCACTGGCGAGTTCTTCAC17280CGGGCTCGGGCCGGTCATCTCGACCGGCACGCAGGCGTTCCTGACGTTGTCCAACGCAGG17340AGCCAACGCGTTCGGACATCTCCTCGCTCCTCTGCAGGAGTTCGCCAACGGTTTCAACGA17400CATGGTCAACCGCGTCACGTCCAACGGCGTGTTCGACGGTGCCATGCAAGGGCTTTCGCA17460GACCCTAGGCAGCATCCTGAACCTGTTCAACCGGCTCATGGAGTCCGGTCTGCAGGCGAT17520GGGTCAGCTCGGTGGTCCGCTGTCGACGCTCGTCAACGGGATCGGTGATCTGTTCATCGC17580GCTGATGCCCGCGCTGACTTCGGTGTCGAGCTTGCTCGGCAACGTCCTCGGGACTCTGGG17640CACTCAGCTCGCTCCGATCATCACGGCGCTGACGCCAGCGTTCACCACGCTGGCCGACAC17700CCTCGGCACGATGCTGACGGGCGCTCTGCAGGCTCTGGGGCCTGTGCTGACCGTGGTCGC17760TGAGACCCTCGGCACTGCGCTTACCACTGCGCTGCAGGCGATTCAGCCGATGCTGCCCAC17820GCTGGTGGACAGCTTCAAGCAGCTCTCCGAAACGCTGGTCACCTCGCTCGGCCCGTACCT17880GCCCCAGATCGGGGAAGCGTTCGGCCAGATCGTAGGTGCGGTCATTCAGTTGGCCCCGAC17940GATCATCTCGTCGCTGATCCCAGCGTTCCAGACGCTGATCCCCGCGATCGCACAGCTCGC18000TCCGTCGCTGGTTCAGATCGTCCAGGCGTTCACCAAGCTGATGCCGGTCATCGTGCCGGT18060GGTCCAGATCGTCATCAACCTGGCTGCGGCCGTGGTGCAGGCTGGCGCGTCCATCGCGTC18120GTTCCTGATCGGTGGCATCTCCCGGCTGGTTGGCGTCCTGGCAGACTGCGTCGGCGCAGT18180CGCCGAGTGGGTCGGCTCCTGGTCCAGCGGTGTGCAGCAGGTCTCCGACTTCGTCGGACA18240GCTCCCCGGCAAGATCAAGAGCTGGTTCGATGACGCAGGCTCCTGGCTGATCGAGGCGGG18300CAAGAACGTCGTCCAGGGTCTGATCAACGGCATCGGTTCGATGATCAGCTCTGCGGTGAG18360CAAGGCCAAGGAACTGGCCAGCAGCGTGAAGAACGCTGTGACCGGCTTCCTCGGGATCCA18420CTCTCCGTCAAGGGTGTTCGCTGAGATCGGTCAGTTCACGGCCGAGGGCTTCGGCAACGG18480CTTCGAGGAAGGGTTCCAGCCCGTCATCGAGAAGGCCAAGGCCCTGGCGGCTGAGCTGTC18540TCAGGCGATGGAGTCTGGCGTGGACCCCTCCGGGATCCTCGCTGGTATCAGCACCAAGGA18600ATTGAAGCAGTACTCGGCTGCGCTGGAGCAGGAGCGCAAGCGGATCCAGGTCGAGAAGAA18660CGCAATTCCCAAGGAGGACAAGGCCGGCCGTGCGGCGCTGCAGGCGCAGCTCGACCAGAT18720CAAGGCGCAGAAGGACATCCTCGCGTACCAGAGGGACCGCATCAAGAACGAGGAGGACTA18780CGTCGGCGCAGCGGGCGATGACCCGCTCGTGAAGGCGGCTTCCGGCTTGATGAACGCACC18840GGTCGACTTCGCGAAAGCGACTGGGAAGCAGTTCCTTACGGACCTGGGTATCTCCGGGGA18900CGGTGCGATCTCCAAGGCCATCACCGAGGGGATTCAGTACATCTTCCAGATCGGCTCTGT18960CGATGAGGCGCTGTCGATCAAGGACCGCGAGGAATCCAAGAACGCGCTGTCGGTCGTGGG19020CCGAGCTTGACATCCACCAGGAGGTAACCATTGATCACCGACACCATCGTTGAACTCGAA19080GGTGTCAACGGTGAGCGCTTCAACTTGACGACCGGTGACCAGGGCGTGTTCCTGGCCACA19140GACGTGGAGGGTTGTTTCTACGACCCTCCCGTCAAGGTCGTCTACGAAGAGCCGGGGAAC19200TACCCCGGTGCTCGCTACCTGGGACACCGAGTTCTGAAGCGCGACATCGTCTTCGGGGTT19260CAGATCCTCAACGACGCGAAGCAGGGACCACGGTCCTGGCTGTCGCGAGACTCCGTGTGG19320CGTAAGGCATGGGCGTTCAACCGCGTCTGCAAGCTCTACGTCACCACCCCGGACTCCGGT19380ACCCGGTACCTGTACCTGGCGCTGTTCGAGTCCCCCAAGGTCGAGATGAAGACCGACCCG19440CGTGGCAACACCATCAACCTGACGGTGATGTCGTGCATCTCGTACGACCCGTTCTGGTAC19500GAGGACGACCGAGTGTTCTCGGTCAAGACCAAGACCGATACCAGGTTCGACCCGAACTTC19560TGGACACCGCCGTGGCCGTGGGAGGAACTGCCCAAGGAGACGCTGCGGATCAAGGTCGGC19620CGCGAGCAGGGCGGGCTCAACCCCACCGACCAGTACATCGCACCGAAGTGGACCGTTCCC19680GGCTCCACCGAGAAGATCCCTGACTTCCCCTGGCCGTTCCCGCCGGGAGTCGAGATCCCG19740TGGGAGACCGCTCCGTTCACGCAGTTCGTCATCCCGGACTACTCGTTCGAGGACGAGGAG19800TTCGCCAACCGCCGGCTCAAGACGCCGGGGTTGATCTACGGCGAGAACTGCATCATCGAC19860ACCGACCGACGCGAGGAGCAGATCAGCTCCGAGTCGGGCTCCCCGGTGTGGGCGCGGATG19920AACGGTGTCCGGTTCCGCAACATGATCCCCCCGTACACAGAGGAGCGTGAGTTCGTCATA19980GACGCATCGGGATGCGCTCCGGGACAAGTGGTTACCCTTCGGCTCCCGAGGCCGTGGTCG20040CGCTGCTGGGGTCTCGAATGAGTGGCCTGACGAGCGTTGCCCAGGCTGAAGATCTCTGGC20100GGAAGATCCAACTGCGGCGCTGCAAGCGCGAGCAGGAGCGACTGAAGCCACCGGACGTAG20160AGCTGCGCGACGGCGACTTCCGTCTGCGCGGCCTCGTCGCGGGTGAGCGACTGCTGGAGT20220GGGAGTTCATCGAGAACGAGACCGGCGTAGCCACGCTGCAGCTCTCGCTGAGCCACTACC20280TGGCCAAGTGGGTGATGAACCACCGGGGTCGAGCAAAGCGCAACGTCATCCTCAACGTCG20340AGAAGCAAGGCGCTCGATGGAGCGGGATGATGGACCACTACCGGGTGGTCAAGGAGGACT20400CCGGGGACTGCTACCTGGAGATCGTGTTTTTGCACGACTTCGAGCAGACCAAGCACATCC20460GTGTCTGGTGCAACCCGTTCCTGCGCCCTGAGCTGCAGTTCCCCAAGATCTGGATCATCT20520TCGGGCCGGCCAAGTGGTGCTTGCTGGTCACGCTGTTCGTGAACCTGCTGCGACTGGAAA20580CGTCCCTGTGGACGATCCCCGACGATCCGACGGACATCTGGGAGTGGATGGGGCCGAGCT20640TCAACCCCAGCAAATGGCGGAACATTGTCAAGCCGTTCCCCTTCCTGCTGGACAACTCGC20700CCATCACGATGGTGTTCAGCCGGTTCGGGACGTTCTACGACACGGCGAAGCAGATCCTTG20760AGAACCACCAGCTCACGCTGACGTGTCGCCGGTACATCAAGGACCGCGACCCGCACCCGT20820TCGATGACCTGAAGGGTCTGTGGGGCATCGACCCCGTCGAAGGTCTGTTGCAGCTCATCC20880CACTTCGGGATGGCTGCGTGGTCTGGGACATCGAGGACAACTCTGGCTGGGGCACCGAGA20940CCGCCTTCGGCGGCTCCTGGCTCACCGGGTTCGTCCGGGCGGTCGTCAACCTGGCTGGAG21000ACGGCCAGGTCGAAGGGGTCGACGTATTCACGGGTGACTACACGTTCCCCGGCGAGTACT21060ACTCCCCGTGGTTCCTGGGCACCAGCCCGAGGGCTCCGCACGTCGTGTTCGAAGAGGGGC21120CACTGACCGGGATCAAGTCGTCGGAGTTCTCGTACTACGAGGCAACCGACACCAGCTTCC21180TGGCCGGTGGACAGTCCGCTCCGGGCATCAACGAGGGGATCTCGGCCCTGGTGAACATCG21240GTGGCGATCTGCTGACCTCGTTCATCAACAGCCAGCTCGCCGTGCTCGGCGCGGTCGGTG21300GCGCGATCGACCTGCCCCCGCTGGGCGGTCTGATGGATGCGGTCCTGAACCCGCTCTACT21360CCGACGTGTTCGGCGCGTTCATGGAAGTCCCGACGCTGCGTGCGATGGGTATCTCGCTTC21420CCATTGCGGGGCTTGAGGACATCGTCACCGGCCTGGGCGACTTCCACTACTACGAGAACA21480TGGTGGACAGCCCGATGAAGGCGTTCACCCTCTCGGCGTTCGCGGCCATCGCAGCCCAGA21540TCCACAAGACCCGAGCCCGAACGGCTCACACGCTCAAGGTGTCTGACGCGGCACCGTACA21600TCTTCGCACCAAAGCCCTACGGGCACTGCTGGATCGGAGATCGCGTCGGCACATCGGTGC21660TCGGCTACCCGGTCGAGCATCAGTTGTTCGTGGAGCGGATCCGAAAGGTCAAGTACCGCA21720TCGACAAAGACGGCATGAAGCCGTTGGAGATCGAGATCGGATACCGCGAACCGAAGAACC21780CAGCTCTGCACATCCTCGAAGAGATCAAGCGTTTCAACGGCGCTATGGGACAAGCGGGGA21840TTCTCTAACCGAAAGGCACGCCGCATGATTCCGTCACAAGAGACTCACAATCCGAACGAC21900CCGCGACAGCACGTCGTCTGGGCGCTCCGCAATCTCCCGTTGATTGCAGGCGTCGGGGCG21960ATCACGCATCCGGCGTACCTGGCGGATTGGTCAGAGCACTTGTGGAAGTGCGGCTTTCGG22020CATGTCGACTGGCTCCGGGAGCTGGCTGATGAGGACGGCAACATCCACGTCAGTCAGCTT22080CCTGACCAGCAGATCAAGTTCCAGCCGGCCTTCCGAGGCCAGCGGCACGACATGAACAAC22140GCAGCGAGGTGGGCCGAGAAGGAACTGGCTCCGGGAGCTGGCTGATGAGGACGGCAACAT22200CCACGTCAGTCAGCTTCCTGACCAGCAGATCAAGTTCCAGCCGGCCTTCCGAGGCCAGCG22260GCACGACATGAACAACGCAGCGAGGTGGGCCGAGAAGGACGCTCCCGACCCAGAACCCGT22320GCGTATCCCAGACATTCGCAAGCTCACAGACCAGGAGAACCGAGCGATGCTCGCACAGTA22380CGAACGAGACGGGTGGATCAAGAACGACCGCCCCGGCCCAGCGATGGCCGAGGTCGTGGA22440GTGACGGAGCTATTCAACCCCGATAACCCTTGGGAGACAGCACTTTTGTTCTTCGCCGTG22500TTCTGTTCGGTACTGCCTGCGTTGCTTCCGTTCTGGTTCAAGATCAAGAAGATCGACAGC22560CAGGTATCGAACTCGCACGACGAGAACCTCCGCGACGAGATCACCCGAGGGTTCAAAGAG22620GTCCGCGAGGACATCCGACTTCTACATGAGGCGCTGAACATCGAGCGCCGCGAACGCATC22680GCTGGTGACGAAAAGAGGTGCGCTTGACATTCCCAACCAACCCACTCGAAGCGATCGGAG22740CTGACGGCGCATTCGAGATCGGCGGCGGTGATTTCAGCTTCGGCCAGGACTACACCGAGC22800AGATCATTCGGTCGCTGTTCACGATGCCGCCTGTCCGACTCGACAACGCGATCACCCTCC22860TCCGCGAGCACCTGCTGAAGCTGCCTCTGGAGGCGCTGCAGAGGTTCAAGGAGATGATCC22920CGGACTGGGCCGAAGGCGCGTTCGACACCGCTACCGGTGCTGTCGATGCGATCATGGACG22980CGCTCAGCGAAGGTCCGCTGTTCCTGAAGCTCGCAGAGTTCCAGGTGTTCCTCCAGCGGC23040TGCTGACCGAACCCGGAGAGGTCATCGGGGAGATCCCCCAGGCTCTGGTCAACGGTCTGA23100CGAGCGCCCTGGAGACCGTGAACAACACGATCCAGACCATCGTGGACATGCTGCTGCAGG23160CCCTCGGCATCAACCCCAAGGGCGACCTGCTTGACCGGATCTTCGACCTCAGCGACGAGA23220TCGAGTGGCTGCGGGACACCGCGAGCCAGGTAGCCTCCGGGCTCCAGCAGACGTGGAACC23280ACTTCTGGTCGGCCCTGACCGGGCGCAGCCCGGGCGAGGACCAGACCGTCGTTGAGCCGG23340CCGAGCAGATCGGTGAGCTGGCCGGTACGACTCAGTCGAACTCGTCTGCCATCGCCGAGC23400TGCAGGCTCGCCTGGACGCACAGGACCACACCGGCATCGCTGGCGGGGATGACTTCGAGC23460GAGTCAACACCACGGCGGTCGGCCCCGGCTGGGCCGAGTTCTATACCGGCGGCGGCTACG23520GATCGGGCAGAGGCTACTACGCGATCAAGGACGGCCATCAGGCCGAGTGGACCGACCAGG23580GAGCCACACAGAACACCGCACGGTTCGTCCGTACGGACCCGGCCGACGAGAAGACGGTCA23640CCGACTACCAGAAGATGACGCTCGTCGTCGGCACCATCCCCGGTGAGGCTGCAGGCCTCT23700TCCGGGGCGGCTCACACATCCGGTTGTGGCTGCGCGTCAACGACAACGCGCCTACGGTCG23760GCATCACCGACGGGGTCTATGTCGAGGTCGGTGGCGCGAACCTCGCGCAGTTGGGCTACC23820GGCGTAGAGGCTCTGACCACTTCGTCGGCTCGGCGTTCAACTGCTCCTGGGGAGCCGGGA23880CCATCTTCACGTTGGTCGCTGGCACGGTCGACGGCATCGAGAAGGTCGAGTTCTACAAGA23940ACGGCTCTCGACTGGCCCTGTGGTCCGACGACGGGCTCCGGTCGGCTATCGGCGCTGGCT24000TCCGGCGCTGGGGCTGGGAAGGCCAGGCCCGTAACCGCAACCTCGGCCAGGGCACCCCGA24060GTTCGGTCACCCGCGTCACGATCAACGACAACGACCCCAGCGGTCTCGGTGGCGGCTCGG24120TCCACCTCGAAACCGGCGTCGTCGGCATCCTGCAGATCCCCAACGGCGGCACAGGTGCTA24180CCAGCGCGGCCGAAGCCCGCGCCAACCTCGGTGCCGAGGCGGCTATCCCGGCAGGGACCG24240TCGCGCAGTACTGGCGCGGCGACAAGACGTGGCAGCCGCTGAATAAGGTCGCTGTAGGGC24300TCCACCTGGTCGACAACACGTCGGATGCCCAGAAGATGTCGGCTCCCGCCGTGCTGACGA24360ACAAGACGATCTCCGGGGCTGACAACACGCTCACCGACATCCCAGTGGCAGCACTGGGTC24420TCGGAGCCGTGAACGCAGTGCGGGTAATCGCGGGCGTTCCGATACCGAACTCTGTGACGA24480TCTGGATCGGGACTGAGGCCCAGTTCCAAGCTCTCCCAACGAAAGACGCCAACACCCTGT24540ACTTCAGGACGGCGTAATGGCGGGGATCTCGTCAGGGCTCGAAGGCATTCGGGCCATCTC24600GTGGAACACCGTCCCCATCCTGAAGGTCAGCCTCGGCAACGACCAGGTGTGGCCAGCGTT24660CGACCCGGTGCTGACTCCGGTCACGGCGGTCGGCGCGTACACCTACAACATCCCGGCACA24720GGCCGAGTTCATCGACGTGATTCTCCTCGGAGCTGGCGGTGGTGGACAAGGCATGGGCTC24780CGCCACTGCGTGGGGCCAAGGCGGCTTCGGCGGTTCCTGGGTGACGGCCACGCTCAGGCG24840TGGCGTCGACATCCCGTGGGCGGTCACACAGATCACTGGCGTCATCGGCGCTGGTGGTAC24900CGCAGGGCCTGGTTACATCTTCGGACAGACCGGCGCGGGCGGCAAAGGGGGCGACACCAC24960AGCCACCTTCTCGGGCGGTGGCACGCTCATCGCTGCCGGTGGTGCTGGAGGCAATTCGAG25020GAAACTGGACTTCGGAGGTAAGTCCCCGAATCCTGCCGACATGGTGTACCGAGACCGGAC25080CTACGACGGCGGCGCTAGGCAGCTCACCCCAAGTGGTATCGGGTACGCACCTGGTGGCGG25140CGGCGCAGCCGCAACGGTCCCAGTGGGTATCACCGGTTTGGCCGGTGGTCCCGGTGCCCG25200TGGCCAGGCGTGGTTCCTGGCGTACTAAGGAGGACGATGGAGTACGACTACGCCCTCCGG25260TACGAGGGCCAACAGACACCCGATGGTCCGTGGGTGGAGGTCATAGTTCCCGCAGCCAGC25320CTCGCAGAGGCTCGGGCCGGGTACGAGGCGAGCCTGCCGACGATGATCGACAACCCCAGC25380ATCCGCAATCTGCAGATCGTCTACACGCCCAAGATCCAATGGACCGTGTGGACTGAGTGA25440CAAGAAACCCCCCTCTTGAGGCTGTATGGCCTTGGGAGGGGGGCTTTTTGCGTTTCAGGG25500GGTAATCCCTGCCAGCTCCGACATTCGCCTCGCTATCTCCTCGTCACGGGCTGCTGAGGC25560CATCTGGTACTTCATCGCCATGCGCGGAGTCGTGTGCCCGAGGCGCACCATCAGCTCCTT25620GGTCGTCGCACCGGCCTGAGCCGCCAGCGTGGCTCCCACGGCCCGGAGGTCGTGGATGCG25680GAGGTCCGGTCGACCGATCTTGGCGTAGCCCTTCTTCAGCGAGCGAGTGAACGCAGACTT25740CGACAGCCGCTGCCCCCGCGTGGTGGTCACCAGGAGAGCTTCCGGCCCCTTGTTCATCTT25800CGTCCGGTCAGCCATGTGCTCGCGGATCATCGCCGCGACGTGAGGCGGCACGGTCACCGG25860CCGCTTGGACCTGACGGTCTTGGTGTTGCCGACGACGATCTTCTCGCCGACGCGGGCCGC25920GCCCCGGCGCACGCGGAGCTTCATCGTCTCGCCGTCATCCACGATGTCCTTGCGGCGGAT25980CTCGATCAGCTCACCGAACCGCAGGCTGGTCCACGCCAGGATGTAGACGGCCACGCGGTA26040GTGCTCGAACACCTCCCCGGCCACTACGTCCAGCTCCTCCGGTGTGAGGGCTTCCACGTC26100GCGCTCAGCGGGTGCCTTCTGCTCGATCCGGCACGGGTTCTCCGACACCAGCTTGTCCTC26160TACAGCGGTATTCATGACCGCCCGGAGTACGTTGTAGGCGTGCCGCCGTGCCGTCGGGTA26220CTGCTTACCCATCCCGGCCCACCACGCCCGGACAAGGGCGGGGGTCATCTCGGCGACCGG26280GGTGTCGCCCAACACCGGGTAGATCCGCTTGCGAGCGTGCGTGCTGTAGAGATCCTTGGT26340GCCGCCAGCGAGGTCTCGCTCGGCGATCCACTTCTTGGTGTACTCCTCGACCGTGATGGC26400ACTCGCCGCAGCCTTCTTCTCGCGCTCGGCCGGCGGGGTCCACTCCTCGTTGTCGATCAG26460CCGCTTCTCAGACGCGAGCCACGCTTCGGCGTCCATCCGGTTGTCGTAGTTCCTCGGCCC26520GAAGTACCGCTGCCCGTCGATCGGGCTGACGTACGACGCTTGCACTCGACCGCTGCGCTG26580GGTCCGCAGCGATCCCCATCCTCTCCGTCTTGCTGCCATGCGAAACAGGCTACCGGAATG26640CGACCTTATTGCGACCTTCAGACGTCTTTGCGTGTCCGTGAACAGGCATTTTTGTCCACT26700TCAAGGTCGCACAGGGTCAGAGCTAAAAACAGGGTCTGAGCTGGGAGAATGTGACACGCC26760GACCCCCTTACTTCCAAACTAGCTACGCGGGTTCGATTCCCGTCGCCCGCTCCACAGGTC26820AGAGGTGGTTTTTAGCCCGAGGACCGGATCCCCGAGAGGGGGCCGCGACCTTAGAGCGAC26880ACTAATGACCTGCATTTATGCTGCCCGGATACAGCGACACATCTGCTACCTTCGACCTCC26940GACAGACGAAGAAAGCCCCCCGCCTGCTGCAACAGACGAGGGGCGGTACACCAGATCGGA27000GCTGGTGCAGTGAAGATTCTCTCACGTGACAAGGTCGCACTTAAGGTCGCAACGGCCGGA27060ACCGTCGCCGTCGGCGGTCTGGCCTTCAGCCTCAGTTTCACCGCGCTGAGCGAGCTGTCA27120GCGGCCAACGGAGTGGCTCAGTCGTGGATGGTCCCGCTCGTTATCGACGGAGGCATCCTC27180GTCGCGACGATGGCGACCGTGGCCCTGAGCCGACACGGTTGGTACGCCTGGGCGCTGCTG27240ATCCTCTCGTCGCTGATGTCGGTCGCGGGCAACGTGGCCCACGCCCAGCCTCACGGGCTC27300ATCGCGATGGTGATCGCGGCGATTCCGCCGTTGTGGCTCCTCGCATCGACGCATCTGACG27360GTCCTCCTCTACCGGGAGGCCCAGGAAAGTGGCTCAGAATCGATCTCAGAGCCTCTTCTG27420ACCAGGGGTTTTGCCGAAGCAGCTTGACTGCGCCCGACCGGGCATAAAGTACATAGACAG27480ACTATGTATTTAGGAGGCACAAAAAAAGGGCTCCAGAAGGGCCAGCACGAAGCCAGCCCC27540TCCAGAGCCCAGAGGGTGCTACCGGGTAGCTCACTTACCGATGGGGCGCATCAGCGCCTC27600GACGGAGTCACGTTCGACGCGGATCAGCCTGGGGCCGAGCCGCACGGCCTTGAGCCGGCC27660GTCGGCGATGTAGCGGCGCACCGTCTTGGTGCTCACGCCGAGGAAGTCGGCGGTCTGTTG27720GATGGATGCTCTCTGCGGCATTCAGCTCTCCTTGGGGTAGACGACGCGCTCGATCCCGGA27780TGCAGCGATCAGGTTCGAGCAGGCGTAGCAGGGCTCTCGGGTGACGTAGAGGGTCGCTCC27840GATGAGGTCTTCGCGGTCGCAGTAGAGCAGTGCGTTTGCCTCAGCGTGGACAGCCACGCA27900TCGAGTTGCTCCGCTGCTGTAGTCACTGACTCCAGGAACCGCCCCAGAGAGTCGGCGAGG27960GCACGTACTGCATCCTGCAGCTCCCGCAGGCGCTCCGTTGTAGCCAGTTCCGCGAACTCG28020TCGGTCCTTGACGACGACTGCACCAACCTTGCTCCTTTCACAGTCCGATCGCTGGGCCGC28080TGCCGTGGCGATCCCGAGGAAGTACTCGTCCCAGTCCGGTCGACTCATCAGAAGAAGATC28140GGCACGTTGACGCCGCCCCCGGCGGGCACGAAGATCACGCCGTTCGGGCCGGTGTAACCG28200GGTGCGGAGCCGCCACCGTCACAGGCCGTGAGGCCCAGGGCGAAAGCGACGACGAGCAGC28260AGGACTGCGATGGTCTTCATGAGGTCTCCTTGTCGAGTTGTCGTTCCAGTTCGAGGATTT28320GGGCCTTGAGCCCTTGGTTCTCCAGCAGCGCCTCGGCGAGCTGGCCTTGTGCGATGTCGT28380TGGCCTCGTCCTTGCGGACGGCCTCGTCGGTAGCGTCGTGCAGTCGACGGATCAGGTCCG28440GGAGGGCTCCGTGGAGACCCGCGACGAAGTCCGCGTCCTGCTCCCGGTGGAATGAGCCGA28500GCCACAGCTTGCTGCCGTCGGTCTGGTCGACAGCGAAGACCTGCCACTGCAGGTGCTCGT28560CACCGTCGAACTCGACCACCCAGTAGCGGGATTTGGCCTCCGTGGTGTGCGACCACTGCT28620GGTACAGCACGTCGAAGAACTCGTGGTCCTCAGCCTCGTACATCGAACTCCTTCATGATT28680CGGTCGAACGCTCGCTGCTGCACGCGCATCTCCAGGGCAACCGTCCGCTTCAGCCACGCC28740CACTCGCCGTCGTGGTTGATCTCCCACTGGCTCTTGAACGCCACATCCTCGACAAGGAAA28800TCGACTGTCAGCGTGTGGATTCCGGTGTTGCTCACCTGGAAGGTGATGCCCTCCTCGGCG28860ATGTACCAGGGCAGCTCCTGGCCGTCGAAGAAGACGGCCCGGTCTGTCACCACGACATCA28920GGAAAATGGTGCATCGTTGATGTACCCCTCCTTGGTGAGGGCCTCGAACACCTGGTCCAC28980GACCTGGTTGACGTGGCCCCCGAACCAGACCGGAGCCATGTGCTCGAACGCAGAGAACGA29040CTTGTGGGTCAGCACCTTGCCGTCGGGAGTCCGAACACGAAACTCGAACTCCCAGTTGAC29100CTTTACGTCGTCGGCCATCAGCGCCGACCCAGGTACGGGATGATGTTGGCCCGGAACTCG29160AAGTAGAAGCCGGGAGGCCGACCTTCGGGGACATCGGCGGTGTAGACGCCGATCTCGTCT29220CCCTCGATCGTGCCTAGCTGGCTGAGCTTGTGGATCGCCAGACCCATCAGCTCCTCGGTG29280AGCTTCCCGTTCGGTGCGGGCAGCACTACGTTGGCTTGTGCCATCAGTTCTTCTCCTTCA29340GTAGTTCTGCGATTGCGATGAGCGCGTTCAACTGCGCCACCTTCAGCTCGAAGTCCGCGT29400CGAAGGTCATCCGACGGCGACGAGCCACCCTCTTGGCTTCTTCCAGGTAGTTGGTGTCTG29460TCAAGTTGCCCTCCTCAGTAATCAGCGCCGTAGAGCGAGCCCCACGAACGCTTTCCAACT29520TCGGGGTCGGTGCCGATCAGCACCGGACCCATCTCCTCGGCCATCAGTCGAGCGATCTCG29580CGAGCGCCCCAGTTGGCCTTCTCAGCGGGCAGAGACGCGACGATCTCGTCGTGGATGGGC29640AGACGTAGGTACGGGGTGAATCCGGCTTCGTGGAGGCGAATCAGCGCCTTGCAGGTCACA29700TCCCGAGACGTGGACTGGATCTGGTAGTTCAGCGCCGAGTACGTCCGAGAGCGGTCCACC29760GGCAGCCGCCGGCCCATCGGGTTGATGATGTACCCGTTGCGCTTCGCCTCGTTGGCGAGC29820TTGCGGCTGTACCGCGTCACTCCGGGATACGCCTTGTCGAACCCGTCCACGACCTGCTTC29880GCGGTCTCGATCGAGATCCCAGTCTGCTCGGCCACCGTGTTGGCCCCACCGCCGTAGACC29940CGGCCGAAGTTCACCGTCTTGGCATACTTCCGCTCCGGGTCGTCCTTGGTGATGTGGTCC30000CCGAAGGCAGCGCGAGCTGTCATCAGGTGGAGGTCCGCACCGTCCTTGAACGCCTGGATC30060ATCGTCCGGTCGCCCGAGAGCGCGGCCAGGACGCGAAGCTCCTGCGCCTGGTAGTCGATC30120GAGGCCATGACGTGACCGGGGTCAGCGAGGAAGCACCGACGCACCGTCCAGTCGGACGCG30180GGCAGCGTCTGCGCCGGGATGCCAGTGATCGACATGCGCGAGGTCCGCGCCTGCAGCGGG30240TTGACGAACGTGTGGCACCGGTCCTCGGAGTCCCGAGTGTCCAGGAACGTCTGGACCCAC30300GACGTGCGCCACTTCCCGAGCTTCTTGGCCTCCTGGACCATCTCGGCCAGGTGAGCTTTC30360TCGCCTCCCGCAGCGATCATGTCCGCGTAGAACCCCTTGTCCACCTTCCGCTTACCGGAG30420TCGGTGAACGCGGTGAACTTGTGTCCCAGCTCCTCGAACGCCTCCGCGACATCCTCGGTC30480GCGTTGACCTTCTCGATCCCGTACTCGTTGAGGAGCACGGCCTCCCAGACCTGCTGCTCC30540CCCAGCCACTTGTCGGCAAGCTGCTGCGAGTACTCCACGTCGAGCAGGAACCCGCGCCGG30600TCGATGTAGCTGCAGATCTCGGAGATCTTGTGCTCGTACGGCACCAACGGCCGGCTCACG30660TCGGGCACCAGCGGTGCCAGCGCACTGCAGATCCGGGCCGTGAAGACCGTGTCCATCCCC30720GCGTACGTCAGGTACTCCGGGTGGTACAGGTCGATGGTCGACCAGATCTTGGCCTTGGTC30780GTCTTGTGCTCCTTGGCCAGCTTGGCCATGAGCCCCTTGACGTTCTCAGCCTGCTCCTTC30840GAGATGAACTCCGCGATCAGCTCCTCCAGCGAGTGGCCGAACCCACCGGCCCCGAAAGGC30900CGGGGGTCCACCAGCTTCGCCAGGATCTGCGTGTCCAGGATCTTAGGCCAGAGCGACTCC30960ATCTTGATCCCGAAGCACCGGTCGAGCACCTGGAGGTCGTACGAGGCGTTCTGCATCACG31020ATCTTGTTCAGCACGTCGAGCGCCTCATGCACCTCCTCCCGCCCCAGCATCTCCAGATCC31080TCGATGGGCAGCACCCAGGATTCGGTCTGAGTACCGAACTGGACTAGGCGGCAACGGAAG31140TCGGCGCTGTAGATGTCCAGCCCGGTGGTTTCGGTGTCGACGGCGAGGCAGTTCTGATGA31200GCCCGGATGAAGTCGCGGAAGCCGTCCAGATCCTCTGGGTGTTCAACGACGTTGATGGTG31260ACGAGGTCTCCTTGGACCTCATGCCGCAGCTCGATCATGCTTCTCCTAGTACGGGTTCCG31320TTCGACCGAGGCCGACGAATGAACGCGAGTGACTGTGTCCCTGTGGACGATTGGGCCGAC31380GACCTCCTTCTTGGCCGTGCCGTCAGCGACGAACCGGGCAGCCAGCAGGTTGTGCTGCCA31440GGTCGTCAGGAGTCCGATCTCGCCACCGAAGTTGATCGTGGCGTTGTCGGTGCCGCCCGT31500GTACCGGACGGTGTTCTCTTCGACATCGGCGACCTCCTTCATCAGGTCAATCGCCATGCG31560CCGCAGGCCAACAGCCTGCACATCGATCGGTAGCGCCTCATCACGCGGCAGCGCGATCAC31620TACCTGGATCCGATCGGCCATCAGTGGTAGATCCCCCGGACGGTGCGCGAGATGGTGGCG31680GGGTTCACGCCGTAGTTGTCGGCGAGATCCTTCTGCTTCATGCCACCCCGGTACGCCTCC31740CGGATGTCCCGGACCTCACGCTCGGTGAGCTTCTGGCGGTTCGGCCGGTTCGGGCCGACC31800GGAGCCAAGCTGCCTTTGACGAACGCTTCACCGAAGGTCCGCCTCGCGGTGTCGAGCTGA31860CTCCGCAGATCGGCGTTCTCCGACCGCAGGTTCTGGTTCACAGCCACCTGGCGGTTGACC31920GCAGTGACCAACCGACCGTTCGCGGCGGTCAGGTCTTCGTTCTTCTGGATCTCCTGCGCC31980AGTTCAACCTTGGCGACCCCGAGGTCTCGGGCGAGCTTGGTGTTCGCTTCTCTCAGGTGC32040TTCTTCCGCATCAGCGGCCTCCCTCTCGACGGAACCGCTCGGTCTCCTCGGCGGTCATGT32100AGTAGAAGTCGAGGACGAAGTCCTAGTTGATGGTTCGGGACGTGCCGTCTTCGAACGCGA32160TGATCAGGACACCCTCTTGGGTGTCGAGGATCGGCTCGCCGTTGAGCACCTTGGTAGCGG32220CCGTTGGCGAGGTTGACGATGGTTGCTCGTTGCGACATGCTCAGCCTCCGTAGCTGTAGG32280GTTCGGTGGGGATGTCCTGGTAGGTGTTGGGAGCGATCTCCCGGAGCTGCCGAAGCAATT32340CCCCTGCCAGTTCCCGGATTTCGGCATCCGCGGCCTCATGCCAGCGGTTCTTGATGACGT32400ACCGCCACGCCCGGTGGTTGCCCGTGACGACCATCGGCGAGTTGGTCATGTTCGGCAGAA32460CGGCCCGAGCCGCTTCACGCGCCTTCTTCCGGGGGAATCCGGCATCGCTGAAGATGTGGA32520CGAGGTACTCGTACGCCTCCTGGGCGAACGACTGAACGTCCAGCAGGACTTCCTTGGCCT32580TGTCGGCGTCGGAGCCCGACAGCTCCGTAAACGCCGGGGGCACATGGATTCCCAGCTCCG32640TGGGATCGACGTACCGCTGCGACACCACCGAGAAGCTCAGGTGCCTGTGACGCTCCAGTT32700CGGTCAGGACCGACCGGCTGGCCTCGATGTAGAACGTCGCGCTGGAATGCTCCAGCACCG32760ACTCATGGCCCACGTCGAGGATGTGGTTGAGGTAGTCGACGTTCTCCCGCGTCTTGGGGT32820TCGGTCGGTCGAAGCTGAGGTAGCAGTTCCGGCCAGCGAACTCCGCGAGTTCGTCTGCCG32880ACGTGACGGTTTCGTCATCACCGACGTAGTCGGTGCCTGCCCAGCTCGGGTCTTCGAGGA32940TCGTGGATGCGATCAGTTGGACTTTCATACTCTCCGCTCAGAGTTGTGAGGGGGCCAGTC33000GTCGTTGCTGGCCCCCGGTGGCTGTCAAGCGTGGATCAGCCGTTTTTCTTGCTGGGGTAC33060TGCGCGGGGCACTGCTCGTTGCGAGGAGCGGTGCAGGAGAACAGCGCGTAGGTGTTCCCC33120GCCTTGGAGACACCGGACTTGAACTCCATCTTGCCGTGCTTGCAGAACCGCTCTTCGCCG33180CCAGGGGCTTCCTGCGCCTGCTGCGGAGCCCGAGACTGGTGCTGCTGGCCACCGCCGCCG33240CTACCGCCGCCACCGGAGTTCGGCTTGCTGCCGCCACCCAGGTTGGCGAAGTGATCGGCC33300ATCTTGGTCACCCGGTCCATCAGGGCGAACCACTTCTGGCCCTGGCCCAGCTTGGCAACC33360TCGTCGGGATCAACGCCCAGGTCGACCAGAGCCTCCGTGACGCTGGCGTACTTCGGCACC33420AGCCACGGCGCGGAGTAGCTGCCGTCACCCTTGAACGTGATCGACAACGCGTCGGCCGGC33480GCAGGCCGCACCTCCACGGTCTGGGTCTGCTGGGCAGGGGGATCCCAGGGCGACTCAGTC33540GGCGTCTGCTGCTGCGCTTCTTCAGCAGGAGCGGTGTCGACCGGCGCGCTGGCGAACGGG33600TCTTCGTAGGACAATTGGTTTCCTCTCACTTAATGGGGCATGCGCCGTTGGCGCACTCTT33660CATCGACACCGTCAGCGACGGCTTTGATCGCAGCAGCCTCGTACTGCTGCTTGGTGATTC33720GCTCGTAGGGAGCCTGCGGGAAGCTCTCCTCCGGGAAGATCGTGGAGCCCTTGATCAGCC33780CAGAGAACCTCTGCAGATCGGCGGCGACATCCACGCCCTCGTAGGCGTCCGGGTCGACGT33840TGGCCGTGAAGCTCACAGCGTTGTCGGCCCAGCACGTCTGGTAGAGCGCCTGGAACGCCA33900GGAGCTGGTGCAGCGTCAGCTCGTTGGCTGACTCCACGATGTCCTCGGCGTCTCGGCCGA33960ACCGTGCGGCCACGGCCTCGACCAAGGTGTCCTTCGTCGGGATCTCGACCACCCAGGTGT34020TGCCTGACTTGTCGTACTGGTCCTTCTCGACGTGGTACCCGTCAGCCGCGTACTGGGACG34080CGGTGAGGAACTGGTCGTTGTCCAGCACCGAGAACCGGATGCGCCGGATGAAGTACCGGG34140AGAAGATCGGGTGGATACCCTCACTGACTCCAGGCATCTTCGCGATGGTCCCTGTCGGGG34200CCACCGTGCGCTTCTTGACCGGCACCGGGATCCGCAACTCATGGCTGAACTCCTCGGCCG34260CTCTGTCGACCTCAGCGGCCATCTCCCGCAAGAAAGCGGTGAACCGCTTGTCTCCGGGTG34320CCTGCGAATACCTTCGGCCTGTCAGGGCCAAATAGGAGGCCACTCCGAGGTGCCCAACAC34380CGATGCGCCGGTTGCGATCCAGAACCTCTCGGCTCTTCGGATCAGCGACCGCTGAGAACG34440TCGCCCGGATCAGGAACCGCGTCATCAGACGGTGGGCTCGGATCAGGTCGATGTAGTCGG34500TCTTCCCGGCGTCGGTAACGAACGCCGCGAGGTTGATGTGCCCTAGGTTGCAGGGCTCCC34560ACGGCTCCAGCGTGATCTCTCCACACGGGTTGGTGCAGACGACCTCGTTGGGCTCACCGA34620CGTTGGACAGGCTGCTGTCCCACATCCCCGGCTCGCCGTTGCGTACGGCTCCCTCGGAGA34680GAGCCTTCAGCACCCGGTGTGCCTTCGTGGAGCGCGGGTTGAGCGGGTCGACAGGAGCCT34740TGGCCAGGCTCCAGAACGCATCGTCCACCTCGACGGAGATGTTCGTGGTCCAGTGAGATC34800CGCTGTCCTGCTTGATGTTGGTGAACGTCTCGACCTGCCAGTCCGCCCAGTGCATCATCG34860CCATGCGAGCCGACCGGCGAACACCGCCTGCCACCACACACTGCGCGATGGCGTGGTCGA34920TCTCCATCGCATCGAGACCGGTCAGCGCACCGCCGTCCTCGAACCTGTACTGCTCGCCTT34980CGCGGGCACACCGGCTCAGGATCTCGGAGACCTCGGTCAGCATCTTCGCCAGAGGCACCG35040GCCCAGAGGCCGTGCCGCCGAACGTCTTCAGCTTGCGACCCGCCGCCCGGACGCGGGACA35100CGTCGTAGACGCGCTCCTTGTGGGCGACATCATCCCGGTAGTGGGTGTCGATCAGGTCGA35160CCAAGGCGGCTGCCCAGCCCTCGCGGGAGTCCTCGATCACGAACGCATCGACCCAGTCAG35220AGTCGTAGCGGCTCGACAACTGACCCGCCTCGGCCAGGTCAGCGTAGTCGTCGTGGTCCT35280CGTCGCAGACGATGTGGACTTCCAGCTCCTGCTTCACGTGCGGGTAGTCAGCGAGGAATC35340GGTTCGAGTAGTTCGCCCCGACGCCACCGCCCTCCATGAGGCGCATGAACGTGAATTCGA35400AGTGGTCCGAGGGCTTCTCGGTCCACCCGCTCACCCAGCAGTTGAACAGGTGCTGGGCGT35460TCTTGACGCCCGACGCCCACAGATGTCGACCAGCGGGCAGGATCTTGAACTCCCGCATGA35520GTCGGAGCAGATCTTCTCGCTCTCCTGGGAGCTGATACCGACTATCAACAAGCGCGAGGT35580TCCCAGAAACCACTCGCTCCACAGTTTCCGGCCAAGTTTCTCGCGTTCCGTCAGGCTTAA35640CCCTGGAGTACGTCCGATTGTAGACAAGCTCTCCTGTTGGCCCCCAAGGGATTTCACCCT35700CTGTCACTACTTCCTCTCAGTCAGTTCGTATGCCTTGAAATAGGCGTCAGCCGAATCGCC35760CTTGGAGAACGAGACACCGTACTCATCGCCACCGATCAGGCCGGTGACAACGACGCCCTT35820CTTGCCCCGGAACCAGCGCCATGTGCCTCTGGCGGGGTACTTGGTCTCGTCGCGCTGGAC35880GATGACCTTGGTGCCCTTCTTCACGTCGGCCTCCGCTGGCCGTAGCCAGGGGTGAACACG35940CCGCCGACGTACTGCTCCAGGTCGTCCTGCGACCAGTTCTCCAGGAGCATCGGCTTCTGG36000TGTGGGAACAGCTCCGGGAACACCTCGGCTCGGTACATCTCCGAGCCAGGCATCCCGTTG36060AACGTGGGATCAAGCAGATTTTGCATAGCACCTCCCTCCCAGGAACTCCGGGATCGGCGG36120CTCGTAGAGGTACTCGTCGCGCAGCCCGGGGTGCTCGACCAGCATGATCGCGATGTTCGC36180CGTCGGGTCAGAGTGGCCCTCCCCCTGCACCTTTCGGATGTCAGGGAAGATGGCGTGCTT36240GCTGCCAGGGCCGTCCTTGACGATGACCTTGCCGTTGTCATCTCGCTCAACACCAGCGGT36300GATCGCGATGATGTTGACGTGCTCGGTGAGCGACTTCACGGCTCGCTTCAGCATCGCCTC36360GGCAGCCGAACCCCTCTCCGGGACGACGCCGTCGTCGTACCGAACCCTGATCGCCTCTGC36420GTGGCGCTCGTTCTTGGAGCCCAGTTCCTTCATCGCCAGCGGCAGGATGTCGACCAGGTA36480CCGATTGGTCGACTCACCCCGCAGCGCGTCCTTGACGTTGTCCGACGAGTAGTGGCTGCG36540CTCCTGGAAGAGATCCCTGGCCTTGGCCGAGCCCGACAGGATGTTGTGGACCTGCTTGCG36600GACGTAGATCACGGCCTCACCGGGCCGCAGCTCGCCGAGCTTCTTCTGGATGTACGGACT36660TTCGAGGTACCAGACCCACAGCTCCTGGACGATCTCGTCCGCTGTCAGGTTGGTCTCCCA36720GCCGATCATCGCTTTGCGGGTGGCCCGCATGAAGAGCTTGTTGATGTCGCCTGTCAAGGC36780ATCACCTTCCGTAGGTACTCCTCCCTGTCCAGACGACGGTCGAGGTTGCGGGTGATCTCC36840TCGGCGAAGACCTCACGGACCTCGCTGGAGCTGATCCGCCGCGACCGTGCGTTCTTGTGC36900AGGTACGGCAGCTTGGTGGCTGTCAAGTTTCAGACCTCCCAGACGTGGCCGTCGACCGAG36960AATCGGCCTCCGACGATCGGAACCAGCTCGGGCTTGACGTGGTTGCCGTCAACCGTCAAC37020AGACCAAACCCGCTCTGCCAGTTGGCCGTTGCACCCTTGAGGTACTGGGCCAGTTTCATG37080TTCATCAGGTTGCCGACCTCCATCGACCACAGCACCTTCTGGTGGCCTCCGTAGCCCCAG37140GTGTGGGGCTTGATGCCTTGCCGGTGGGTGTGACCGATGATCACCGACGTGCCGAACCGC37200TGCATCGCGTTGTACGCGGTGTCAGCCGACTTCTGCGTCACCCGGACGCCGCCACGGTGG37260CCGTGCGTCGAGATCCAGCCCGGAGCCACCTTGTAGAACTCAGGCAGCACCGTCACGCCG37320AAACCGTCGAAGTCCAAGAGGTTCTCGAACTTGAACTGGTCGGCGTACTCGACCAGAGCT37380GGTGCGAACTTGTGCAGATAGTCGAACGGCCGGCGGTCGTGGTTGCCTTCGTGGACGCCG37440ACAGGGCCGTCGTACACGGCCCGCAGCGGGGCCAGGAACCTCGTCTTGGCCTGCTCCGAA37500TCGGGCTTGATCCGCTGGGCGAACTCCTCCGACGAACCCTTGGTCCACCGAGACGGGCTC37560GGGTAGTCCATCAGGTCGCCGATGTGGACGACCTCGTCGGGCTGGGTGTCGCCGATGAAC37620CCGACCACAGCCTTGAGCTGCTTGCGGTCATCGAACGGGATCTGCGTGTCGGAGATGACG37680ACGATGCGCTTACTCACCCAGTACCTCCACGAACGGACCCTCGGAGATCTTGGCGTGGTC37740GAGCTTGAAACCGTCGCGGTGCCCCCACAGGCGCTCGTTGTAGTCGGGGTACCGCGACCA37800GAACGCGTTGCCCGCGCTCCGCACGGTCTTCACGTCCTTCGGTACGTCGGCCAAGGTGTC37860CCACTGGCGAGGTTCGCCCTCGACAGGCTCCTCGTAGATCCGCTCGACGCAACCGGCGTA37920ACCGGCCATGTCGGTGAACGAATCACGGTGGTAGCCGGTGCCTTTGACCCTGGCGATCTT37980CATCAGGATCATCAGGTTGGCCACGTCGATGTCGGAGATCTCCTTCTCCAGGTAGCCCGA38040GAACAGCAGGGCGATGTCCTTGAAGTTCTCTCGCGGGTGGCCGTAGTTCTTGTTGCGCTC38100ACCGTGGATGAGGCGCTGAGCTTCCTCGAGGATGGATTCACTCACAGTCCGGTCTCCGAT38160GCGGTGTAGTAGTCGATCAACTCATCGACCTTGTCGGGTTGATAGCCGATGATCGGCTCG38220AAATGGTCGGTCACGATGACCGGAACCGAGGACGCCTTCAGTACGTCCTGGACGTACGTC38280TTGGCCTCGGCGTTGCGCGTCAGGTCGACCACGTCGTACTCGACCCCAGCGTCGTCCAGG38340AACTGCTGGATCCGGTGGCACGGGCGGCAACCCGGCTGGGTGTAGATCGTGATCGGTGTG38400AACATCGTTCGCATCAGATCCTTTCCAGCAGAGCGTCTTTGCCCTGCGATGTGACTAGTG38460AGTTGACATCCTCGCCTTCTGGCATGGGGATGATTCGTGCGTTCGGCAGCGTCTTGGCCA38520CCGACTTCGCGAACTCCATACCGGGCTCGTCGCCGTCAGCCAGGATGTTCACGTTGCGAT38580ACCCGAGGAACAGCTCGCGGAAGTGCGGCTTCCACATCTGAGCCCCTGGAACGCCGACCG38640TCGGGATCCCGCACAGCTCAGCGGTAATCGCGTCGATCTCACCTTCGGTGATCGCCATGT38700CCCGCGAGTAGCGGGTCAGTGCGACCGTGTTGTACAGCCGGGGCTTATCCCCCGGCATGG38760TCATGTACTTCGGCTTGCCGTCGTCCAGACGGCGATACCGGATCGCTGCAACCGACCAGT38820TACGCCAGGGCGACCACCGCATGTACGGGATCGCCAGGCAGCCTCGGTACATCTCATGAC38880CAGGGAGTGGATCTCCCACGAATCCCAGGCCGAACGGTAGAACCTGATGAACCTGAAGAC38940CGCGACTCTCCAAATACTCGGCGGCTGGGCTTCCGTGCAGGCTTCCTCTGTACTGGGATG39000TTGCCTCCCACAGATAGTCCCTCTGCGATTCGGACAGCCTCTGCAAAACTCACCTCCTCT39060TCGTGTCGGATGATCGAGATCACGTCCCCTCGGACTCCGCAGGCCAGGCAGTTGAACCCT39120TGGAGGTCGTAACTGACTGCGGCCGAGGGTGTTTCGTCCCCGTGGAAGGGGCACAGGCAC39180TTGTTCCACTCGTAGTGATCCGGTGGAGGATCCCAGTCCGGGTAGTACCGCTGGATGACC39240TCCGCGATGCCGGTGGCTGTCAAGTTTGACTCCTCAGCTTGTCGGCCTCGATCGGCGCTA39300TGCGCTCCCCGATGACCTGGACGGCCGGGGGCTTACGCAGGTAGTCGATCGCTCGCTGGA39360GCATCTCGATGCAATCCCGCGCCCAGCCGAGGATGTACTTGTTGCACATCGTGCAGAGCA39420GCCCTCGAACGATGCCCGTCTTGTGGTCATGGTCTACCGATAGCCGTTTCCTCTTTCCGT39480TCGCCCGCTGGCAGATGTAGCAGCGGCCACCCTGGAACTCATAGATGGCCCAGTACTCTT39540CAGCGGTGATGCCGTAGGTGGCCAGGATCCGGGTCTCCCAGCTCGTTGAGCTGCGAGCAG39600CCTTGAACTCCCGGTGGTGCGTGGCGCACCGTGGGCCGGGATACTTGGCGTCCCGTGTGA39660GCGGCAACCCTTGGGCCACACAGTCTTTGCACGGCTTCCGTTTGTGCTTACGATTCTGCA39720CCCGGTACCCCGGAGTCCTCTTCGCGGCTCTCCCCATCGCTCCCCTTCGGGTTGTCCATC39780AGCAGGCAGAAGTACGACAGCACCGCACACGCGATGATGTGTGCGTCGAGCGCGAACAGC39840ACCGGGTTCATCCGGCACCTGTGATGTATCGCCGTGGGAACAGATCCAGCAGAGCCAGCG39900CGATGAGATCAGCCGCCAGCTCCGGGTCAGCCAGCATCCATGAGTGGAAGCCGTCGACTC39960CGTAGAAGGAGGCGTTGGTGATCTTGGCCGTGCTCAGGCCGGCCTCGTACGGAACGATCT40020GATCATGCAGCCCGTGGAGCACGGCGGTCGGTACGCCGTGGCGGTACATCGCGTGCAGCA40080GCGGTGTCGTGTCGGCCTTCATCAGCGCGAACGCAGCGCGAACGAACCTGAGCCCGGACA40140CCGAATCCCGCAGCATTCCAAGGAAGCTCAGCTTCTCGCCGGTATCTCGCAGGTGCATGG40200CCTCGTAGCCGTCGCCGACGATGTCGGTCACGGCCTCCCCGAGCTTCTTGGCAGCTCGGA40260ACGCCAGCGTGGCGTAGTGGCCCACCTTGATGTTGTCGTGGTGTTCCTGGCCAGCAGCCG40320CGTCCAGCAGGACCGCTGCCGCGACTCTGTGCGGGTGACGCGCCGCGATTTCGACCACCA40380TGCCGCCCCCCATCGAGTGGCCCGCGAAGATCGCTCGGTGGATGTCAAGTTCGTCCAACG40440TCTTGAGGGTCACGCGGGTCATGTCCTCGACCGTGTGGCCGGTCGGCAGTGAGCCGCTGC40500GGCCGTGGTTGGCCGCGTCGAGTGCGATGACTCGGAACCCGTGCTCGGCGAGCCGGGTCA40560GCATCTCCTCGTACGCCTTGGCGCTCACCGAGAGCCCGTGCAGGAACACCAGCGGGGCGC40620CGGTGCCTACCGTTGAGACCCCGACGCGGAAACCGTCAGCGAGGACGATGGTTTCGTGCT40680TCATGGCTTGTCTCCGAAGTTGATGACAGGAATGCCAGCCTTCTGGGCCTCACGCATGCA40740GTGGCGCGTGCCGACTGACCTACCGAGAGGGAACGCCAGGCAGACATCAGCCCCAGCCCT40800CACCATCTCGATGTTGCGGAGGACGCCAGCCTTCTTGCCGTGCCACTCCCAGTCCGCACG40860GTGCAGCTCCGGGGTGACGTTGAAGCCTTCCTGGCGCATCCCCCAAGCCCAGCGGTCAGC40920GATGTCGTCAGCGCCGCGAGCGCCGCCGTGAACCACCACCAGGCCATACGGGGACCGGTG40980CAGCTCGTCAGCCAGGGCGTCCCACACCGTGGTGCGGTCCTTCCAGACTCGCGAGCCGGT41040GATCAGTACCCGACGCATCAGATCTCCCTCCAATGCAGCCCGTCCTCGGACTCGACCAAC41100TTCGCGCCGTAGACGCCGTTGATGATCGCCAGGTACTCGATCTGCTGGGCCTGCAGAATC41160CCGAACGGGCATTCGTGAACTCCGCTGCGCGGGTAGCGGACTCCGTACTTCACTTGATCC41220ACCTCTTGGCCAGTCGGTCGACGTTCTCCTCCGAGACGTTGCGGGCCAGGCCGGTGACCT41280CGCGGCCGTGGACCTTGGTCTCGATCACCCGAGGCTTCTTCGGATCCGGGCTCTCCGGGT41340CGATCGCCTTGTGCGTCCAGACGGTCGGACGCGTCTTGATCAGCGCGCCCAGCACCTGCT41400GGTGCAGCGGGTTGGCCTTGCGGGGCATGGCGTTCGGAGTGGTCATCTGGGTGTTCCTTT41460CGGTGGCTGTCAAGGGATGGATGAAAAGGGTTGGGGCACATGACCGTTTGTCGTTTAAGC41520CAGGGCGGTTCCCGTGCGCTCGCCTTACCGAGCAGACGCGAGAGCCTAGGTCACGTACGG41580TGACGCCCTGCGCCCCCAACCCCCTTGGTCAACGAAGCTCGATGTCCGGGAGGATGGACT41640GCGGCTTGAAGTTGACCTGGTAGAAGTCGTCGGAGACGTTCGCGCCTTCGATCTGCTCCA41700CGAAGTACGTGACGTTGTCCGACAGGCCCAGGAAGTGCTTCTTGAACTGGCCGTTCTGCT41760TGCAGGTCACGTCCAGCTTCTGGGCTCCGGTGTCGGGCTCGATGGAGCACCGACCCTGGA41820TCTCCAGCAGGTACTTATCGGTGATCCCGTTGAAGAACACGATCCGGCGCGGGATCTCGA41880AGTTGTCAGCGGCCTTGCTCAGGTTCTCTGAGGCCACGTCGGCGTCGGAGGTACACCCGA41940CCAAGCCCAGACCGGCGGCGAGGGCGATGGCTGCGGTGGCGATGGCTTTCTTCATGTGCG42000CTACTTTCTGGTTGGTGGCTGTCAAGTCAGTGATCGAAGTCGTTGATCTGCATGGTGTCT42060CCGATGAACTCCAAGGCGGCGTAGTCATTCCCCGACGGATCAGACTTGCCACCCCGGTTC42120TTGACCGTGGAGACGTTGAGCGAGTCCGGGCCGAAGCCGTCCGACTCGCGATGGAGCGTG42180AGGACCATCTCGGGCACACGCCCGATCTGACCCTTGATCCCTCCTAGCGGGATCGCTTTG42240TCGCCGTCGTTGTACGGGCCGGTGACGTGGTGGAGCCCGATCACGCAAGAGCCAGTCTCC42300CTGGCCATTTCGTGCAGGTAGTCCATCAGCGACTCCAGGCCGCTGAACGGGTCATCCCCG42360TCCCCCGACTCGGTGCGGACGTTGGTGATGTTGTCCACGACGATCAGCGCAGGGAAGTCC42420TCGTAGAGCGCGTCGTACGCTGCGAGGGCGTTCTCGATCTCGTCCAACGACGGAGATGCC42480TTGTAGTTGAACCGGATCGGGATCTCATCGAGGTCGTTGGCAATCGACTCCTCGATGCTC42540TGTTCCCGGACTGCCCGCGTCGATCGCTCGAGCGACCATCCGCTCAGGATGGAGACTGAC42600CGCGAGATCTGGGTGAACGCGTCGGAGTCCGCCGAGAAGTACAGCGTCGGTACCTTCGAC42660TTCAGCGCGTAAGCCAGGACAAAAGCCGATTTACCAGTGCCAGGACCGGCGCATACAAGG42720ACCAACTGACCTCGTCGTAGACGAGTCCCCTTCTGATCAAGTGCGTTCCACACCGTTGGG42780AGTGGGTCACCCGCGTTCCCTCGGATGTACAGGCTCTGCCTCGGTGTGTACAGCGTCCTC42840CTCCTTTTCTCGTTCGAGCATCTCCCGGAGCATCCAACCGGGGATGACGTTGTTGAAGCT42900CACAGACCCTCCTCGATGTCGTTGACCATCTCGCGGACCTCGTCCATCGGCACCAGGTCG42960TGCCAGGCGTCCAGCCCGACGTGGATCTGCCGTGGGTTGGTCATGGTCGACCGAATGATC43020CGGGAGTGCGTGTGGCCGTGCAGTAGGATCAATCCTTCATCCCGCAGCCGCCACTGCGTG43080AAGCGTTGCTCGGCGGTGTGGTCGCCGACGTACGGGAAGTGGCTCAGCAGCACGTTGGTG43140TGACCGCCTCCGTCGAGGGGGACGCGAAGCCTCGCGGCCGTCGACATGTAATCGAGCACG43200TTCCAGTACAGCCGGGACAGCCGGGGAGCGTCCCGGTACATCGGGTGAGGCCGATCATGG43260TTGCCCAGGATCAGCCGCTTGCGGCCTGGCCGGTTCAGCAGCCAGCCCAGAGCATCGAGC43320TGCGCTCGGGTGCCGCCTGAGCTGATGTCCCCCAGGATCCAGACCACATCTTCCTTGCCG43380ACCTGTTCGTCCCACAGCTCCGCGAGGTGGAGGTCGTGGTCCGGGCCAGCCCAGTCTCGG43440TCCTCCGCTACCTTGGCGTGGCCGATGTGTAGGTCGGAGGTGAACCAGACGTTGCTCACG43500CTGGCTCCCTCACGTCGGGCCGACCGTGGGTCTGCCAGATGAACTCTTCGATCTGGTCGA43560CCGCGATGAAGAGGTCATCCCCGTCGATCTTCGTCGGGAGCCCCATCAGGATCGCCAGGA43620TCTTGTCTCGGATCACTGCTTGACCACCCCCGCGTCATGGATCGGCCGGCCAGCTCGGTG43680CGCCGCCTGCTCCGCGTCCATCGCTCGCTGGATCTGGTTCATCAGTTGGGTGCCTCGAAG43740CTTCAGCAGCTTCATGGTGTGAGCACCCTTGAATCCGACGCGGTGCATCCGCAGGACCGC43800CGCCGTCTCATGCGGCGCGTACGGCGACTTCAGCTTCGGGTCGTTGGGATCCCAATCCTT43860CTTCATGCCTTCTCCTCTTGGTGGCTGTCAAGTTCGATGACGATGTTCACGCGACCCACT43920CCTTGCCGTTCCACCGCCGACCATCTGGGTAGGCGATGATGACTTCCCGGTCGGGCCGCT43980CCTCGCTGTGAGCGCGAGCGAACCGGAACGCCGCTTCTGGCGTCGGGAACGGCCAGCGCG44040AGGGCTGGGCCGACTGGTGCCACTTCGGCATGTCTGGGATCGGACCCAGCTCCACGTAGG44100TGTAGCTCGTCCCGGTGCTGAGATCGAGCGTCTTCCGGTATTCCTTCATGCCTGCCTTAA44160TGGGGAAGGAGTCTGCGGGTGGCTGTCAAGTTGCGATCACTTAAAAACGGGGCAACTGTA44220GGACACATCACAGAATTTGCATTTGTCCGGTTCCGGCAACGGGTCGAACATCGAGGCTGC44280GATGCCTTCCTCGACCCTGTGGAACTCCTCGGTGATGGCTTCACGCGTCCACTTCGTCAG44340GTCGTAGGGCTTGGTCGGCTTCGGTGCCTTGCCCTTCTTCCCCACCATGAAGTAGTCGCC44400GGTCTTCGGAGCCTCGACACCGTAAGTCATCGCGACCGCGAGCGCGTACACGCCGAGCTG44460GAAGTCGTCTCCCGGTTGGTTCCCGGTCTTGTAGTCCCGGACTCGAAGCTCCCCGTCGAC44520CACGACGACGGCGTCGATGTATCCGCGAACCAGGATCCCGTCGAGTTCGATTTCGAAGTA44580CAGCTCGATCGCTGGCGTACCGTCCGGGGTCACCCAGATCTCCTGGCCGGGAGTCCCCCG44640CCACGCGAAGAACTTCTCGACCTGCTCCAGGCCGACGGTGTACCGGCGCTCGATGTCTCG44700CTGGCCGTTGTACGGACCTGACCACGTCCACCACTCGAAGTTCGGGGTCTCCTCGGTGAA44760GGCTCCAACGTCGTTGGCGTAGTGCCAGCGGAACATGTCCTTGGCCTCGTCAAGCGTAAG44820CGGCATGCCGTAGGACTCCCATACCTCGACCGCCTCGGCGACAGCGTGGAACGCGGTCCC44880CTGCGGCAGCCAGGCCGCTGGCCGCTGCCACACCTTGTCGATCCGAGCGAGCTTGTACGC44940CATCGGGCACTTCGTGTACTGGTTGATCTGGCTCACCGAGCGCAGCGGAAGCTTGATCTG45000CGTCACGGCGCGGCCATTTCGCCGTTGCCGGTCAACGCGGTCTCCACCGTCGAGCCCACG45060ACCTCTCGGATCTCATCCAGCGATGGGCACATCGGGGACTCAACGAAACTGTGTGGCCTC45120AGCCTGGCTACCAAGAACTCTCCTCTCCCGTGCATCATGTTGTCGTCATGCCGGAAGCCA45180CGTGCGAGCACGTCAACCTGGTCGGCCTTCAGAATCTCCTCGGCGACAGGCCGATACATC45240GGCTTCGTGTCTCGGCACAGCGGGCTCCGGTACGCGAGCAGCAGGGTGACAACCTGGGAA45300GGATCGTCCTCCATCCAAGCCTTGCACTTCCATGCTTCCAGCCACCCGTGACGCTCGACC45360AGCTTCTCTGGCTGCGTGTAAACGACCCCCTCGAACTGGCCTACAAGGCTAGTGAGCGGC45420ATTGCGGTTCCTTACTGCGTGGGCTCCGCTGGCTACGGAGAACGCACGCGCGTCGTGTTA45480GTTCAGGAGTTCCTCGATGTCGGGTGGCCAGCACCAGATCATCTCTCCCTCGTCTGTCAA45540GTTGGTGTACTCGTTGACCCGGATCAACAGGTCGTCGTCCTCGATGGTTCTCGGGACGTA45600CCTGAAGCCTCCACCGGCCATGCCGGGGTAGGGCTCAATGTTGGGGTCGAACTCGACTAC45660CACATCGTTGTCGCGCAGCATCTTCCACCACGACAACAGGCGCTTCCGCTTGTCCTCTGA45720CATCGTCTTGAAGCTGCCCACGCGCATGTACTCACCGTGGTCACGAAGTCTCTGGTAGGC45780CTTGGACTTCCCCTGAAACTTCGTCGTCTGCCACGGCCAGGCCAGCGTGGACGTTCTTCT45840TGCCTTCACGGCGCACACGCTCACGGCCGGCCGGCCTCACCACACCTTTCGCGTGGGCCA45900GCAGAACGTGGCCGCTGCGGTGGATGACTCGACCCTTGAAGTCGCCTTCCAAGGCTTGGA45960CGGAGTACCACGGCTTGCCCTCACGGTGCGTGAAGTGCAGGTTCTTGTAGACGAAGACGC46020GAATTGGCTTGGGGTTCATGCGATCCAGTCATCCAGGTAGTTGTCTTTGACGCTGAAGCG46080TCGGGTCCGACTCAAGACTTCGATGGCCGCGAGGATCTCGCGGTCCTCGCCTTCGCGCTC46140CATCGCTTCGTCTTCGACGTTGATCACTTCGATCCCTTCGGCTTGGCAGGCGGTGTCTTC46200CAGGGGTTCATGCCCAGCCGCCGAGCGCGGCGCATGGCAACCATGATCTCGACGTTCATG46260AGGCCGAAACCTCCCACGTACCAACGGCTTCGCCGCGAAGGTAGATGTGACCTCCATCGC46320CGAGCTGGTCGATGACCAGGCCGTTGCGCGAAGCGGCAGCTTCGAGGTGGTCGATGAGGA46380TCTGGCGATGGCTCGCCGATACCTTCGTATTCTTGCCGTCGCGGTCACGGGTGGCAGTCA46440AGGTGAACACAGGTCAGGTACCTCTCAGTAGTCGGAGTGCTGGAGTTCGAAGCCTTCGAG46500GTCACCGACCTCGTCGTCCCACGCGGACGGGTTGCCGCGCCAGTCGTCGCGAAGCCTCTG46560GCCGCTGGCGTTGTAGCAGGCACCGCAGTTGCGACAGTCCACGTCGCTCTGGCCTCGGTA46620CCTCTGCGCCTCATGGCCGCACCGTGAGCAGTCCCACGCGGCGTAATCGCCGTCGATGAT46680GAACCCTTCGGCGTCACGCACAGCTTCGACGTAGCGGTAGTTGTTGGCCATGATCAGCTC46740CCCAGCTCGTTGGCGATGGCTTGGTGGGCAGCCTCACGGAAGGTCAGCCCGTCGTCGTAC46800GCGTCGTGGTAGGTCCAGTCCGCGATGTCTCGTCGGCCGACCCCGAACGTCTCTTGCATG46860TAGGCGTCCAGGTTGGCCATCCACAGGTGGAAGCTCACAGCTACCTCACTTCTTGGTGGT46920GGAGAACAGAACGCTCTTGCGTCCGTTGATGCACAGACCGCACCTAGCGCAGGCCGATCC46980CTTGGCGTTGATCAGGTCGAAGTCTCGGTTGTTCAGCTCCGGGCAGCGCACGGCCGTCGG47040GAACTGTGCCTTGCCTTCGGCGAACGTCGTGTCGACGTAGGCGATGTTGATGCCCTTGCC47100TTCCAGGAACCGGGCCACGTCTACGTTGTCGGGATCCGCGCTGAAGTACAGCGACAGGTT47160CGACAGCTTCTGTGCGTGCAGGTAGACCGCTGCGGTCTGAACCCTGGTGTAGGCCCAGAA47220CTGCACGTCGCTGAAGTCTCGGATGACACGCGCCCACGCTGCGACGTAGGTCGGGCTGAA47280GAAGTCGCCATCCCAGTGGATACGGAACAGCTTCGGAGCCTTCTTCTTGTCGCAATCCTT47340GACGAAGTCTGCCATCATCTCGGACAGCAGGGTCACGGTGTCTGTCAAGTCAGCGTCACG47400CAACAGCTCCCAGTTGTGCAGCAGCACACTGCTCACGGCTTTGCGGACCTTCTCCAGCTT47460GCCCGCGTAGCACACCTTGGCACAGAACGCGGTCGCGTCCGGGCAGGAGAACCCTTGCCC47520ACTGGGCAGACCGATGCTGTTCGCGATGCCTACGGTGGCGTTGCCGCCTTTGGTGACGTG47580GACGTAGTTGGTGACTTTGCGGTCGTTCGATCGCTTCAGCGATGCCATGTCTAGAACCCT47640TCGGTGGCTGTCAAGTCAGCGGACGCGAATGATGTTGGTGCCTCGGCAGACGTAGATCTT47700GCCGTCGATGTACACCTTGCGCTCGGAGGACATAGTGAATCCTTTCTCGGTGGCTGTCAA47760GTCTCAGGCCCAGCGACGTTTCGTCGGCCGGGGGTGGCGAACCTTCGGAGCGTTCTTACG47820CGGTGCCTTGCGGATGACGGTGTCGCCAGTGATCGTGATGTCTTCGACCAGGGCAGCCTC47880ACGCTCTGCAGCGATCTCGGAGGTCGGGACGTTGACCACTTCCGGCTTGTTGCTGAAGTC47940AGTCCACCCCTTGGTGCCCTTCTCCAGCTCAGCGTCGACGGTGCGAACCGTCGACAGCTC48000AGGTGCGACGAACGGTGTCTTGATGGACTCGCGTGCCGTGACGCGAACCTCGCGGTGCTC48060GGTGAAGACGGACATAGCTCACCCCTTCCGGTAGCTGTCAAGTCAAGAATCAAAGCTCAG48120GTAGTGGGTAGCCAGGAATCGAACCTGGTAGTCGTGATGCCGCGATACCAAAGCTCAGGT48180ATCCGCCAGACCATAGCTCAGCGATCATTCCATCGCGCCAGAGCTACCCGGTAGTCTTTT48240GTTCTCCCCGTGGGTCAACAGAATCTATCGGATCCTCGCCACAGGTCTACATTCAGTTAT48300CCGCAGTGTCCGCACTTTATCGGCATCGGACTTCCGCCTCACCCGCGAATGCTGTCGAAG48360CCATTCGCGAATGTTGGGTCGGGCTGCGGCCCTTCCCGGTCTTGCGTGATTCTCACTCTA48420CCGGACTAGTCGGTGGCTGTCAAGCGGGCCGTTTTGGTATCGGCATCGATGCCCTCGTTT48480AGCGCCGCTGGCATAAGGCGCTACCCGCTCGACTCACCGGTCCAAGTTGGTGATGACATT48540CACTCTAGCGTATCGCTCGGTGGCTGTCAACCGGAGAATCACACCGGATTTTCACCGGAT48600CCGGCGCGATCGTTTCCGATCCGCCTATCGCGTCGTGCTTGCTGCGATGACACAAGTAAA48660CACCACGGCCGGGTAGCTGTCAAGCCCGAATTGCAAATTGGTGTGTGACGTGCGGTTACG48720CCGCTGTCCGGGTGGCTGTAAAGGGCACGTAGGGCACGTGTGGCAGGCCAGACGCAACCC48780ACTAGCGGCCGGTAGCCGCGTGCATAGGCTGCTCGTTATGTGCCCGGTATGGGTGCTGTG48840AGCTGCACAATCGCGGGCTGTCGGGCTGTCGTCACGCTGTCGCTGTCGTCGCCGGGTGGC48900TGTCCAGTCGCCCACCGAAATAAGCGAAATAAGCACCGTCGTCGCAGGTCCATAGGCTGC48960TCACTATCGCATCGGTATGCCCTTGCACACGTGTGTGAGCTGGTCACGTGCTGGTGTGGT49020GTGCCGTGTGCGCTGGTGTGTATGCGCTGGTCAGCGTATGGGCACAGTGTGCGTGTGAGT49080TAGCTGTGAGCTGAGGCCGGCATTCGCATCGTCGCAGGTCAGCACGCGTATGCGTGCATG49140TGTCCTCGGTTGCTGGGCATCGTGTGCCCCTCGAGGCACGCGTGCCGTGAGCGTTTGCTG49200TGCATGCCATCGTCGCAGGTCACGGGGGGTAGGGGGGTTCCCCCCAGGGGCGCCTTCCTG49260ACCGGTCGGTTA49272__________________________________________________________________________

Claims

1. A purified and isolated nucleic acid encoding D29 mycobacteriophage having the sequence of SEO ID NO: 1.

2. A shuttle phasmid comprising a D29 mycobacteriophage which contains an E. coli bacteriophage lambda cosmid inserted into a non-essential region of said D29 mycobacteriophage.

3. The shuttle phasmid of claim 2 wherein said cosmid is excised by PacI.

4. The shuttle phasmid of claim 2, deposited under ATCC Accession No. 97468 and designated phAE70.

5. The shuttle phasmid of claim 2 further comprising a DNA of interest inserted into said E. coli bacteriophage lambda cosmid.

6. The shuttle phasmid of claim 3, that replicates at a temperature of 30.degree. C., but not at 37.degree. C.

7. The shuttle phasmid of claim 5, wherein said DNA of interest is a transposon.

8. The shuttle phasmid of claim 5, wherein said DNA of interest encodes a protein or polypeptide selected from the group consisting of: antigens, enzymes, lymphokines, immunopotentiators, selectable markers and reporter molecules.

9. A recombinant mycobacterium which expresses a DNA of interest introduced into its chromosome by the shuttle phasmid of claim 5.

10. An auxotrophic mutant produced by infecting a mycobacterium with the shuttle phasmid of claim 7, such that said transposon incorporates randomly or site-specifically into the genome of said mycobacterium thereby generating an auxotrophic mutant.

11. A method for producing a mutant mycobacterium comprising infecting a mycobacterium with the shuttle phasmid of claim 9 under conditions such that said transposon randomly or site-specifically inserts itself into the genome of said mycobacterium, thereby generating a mutation in said mycobacterium.

12. The recombinant mycobacterium of claim 9, wherein said DNA of interest encodes one or more proteins or polypeptides selected from the group consisting of: antigens, enzymes, lymphokines, immunopotentiators, selectable markers and reporter molecules.

13. The recombinant mycobacterium of claim 9, wherein said DNA of interest is a transposon.

14. The recombinant mycobacterium of claim 9, which is M. tuberculosis, M. bovis, BCG, or M. avium.

15. The auxotrophic mutant of claim 10 which is M. tuberculosis, M. bovis, BCG, or M. avium.