Claims
- 1. A debranching enzyme product comprising the debranching enzyme from a strain of Bacillus acidopullulyticus said enzyme being further characterized by:
- (a) immunochemical properties identical or partially identical to those of the debranching enzyme derived from the Bacillus strain NCIB 11607 or NCIB 11639,
- (b) activity optimum, measured by incubation for 30 minutes in acetate buffer of 0.05M at pH 4-5 at about 60.degree. C.,
- (c) pH optimum in the range of 3.5 to 5.5 as determined by incubation for 30 minutes in acetate buffer of 0.05M at about 60.degree. C.,
- (d) a residual activity after 72 hours at 60.degree. C. as measured in a dextrose solution of 30 percent D.S. by weight at pH 5 of at least 50 percent.
- 2. The debranching enzyme product of claim 1 in which the debranching enzyme activity is in the range of from 10 to 350,000 pullulanase units per g.
- 3. The debranching enzyme product of claim 2 wherein the activity is in the range of from 100 to 15,000 pullulanase units per g.
- 4. A process for the preparation of a debranching enzyme which process comprises cultivating in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts, a debranching enzyme producing strain of a microorganism Bacillus acidopullulyticus, and thereafter recovering a debranching enzyme product from the medium, the Bacillus acidopullulyticus strain being selected from the group consisting of NCIB 11607, NCIB 11610, NCIB 11611, NCIB 11636, NCIB 11637, and NCIB 11639 and mutants thereof.
- 5. The process of claim 4 wherein the strain is selected from the group of mutant microorganism strains consisting of NCIB 11638, NCIB 11647, and NCIB 11777.
- 6. A biologically pure culture of a strain of the microorganism Bacillus acidopullulyticus selected from the group consisting of NCIB 11607, NCIB 11610, NCIB 11611, NCIB 11636, NCIB 11637, and NCIB 11639 and mutants thereof.
- 7. A process for converting starch into syrups containing dextrose and/or maltose, which process comprises conducting the saccharification of starch hydrolysate in the presence of an enzyme system which comprises effective amounts of the novel debranching enzyme product defined in claim 2 and a saccharifying enzyme selected from the group consisting of gluco-amylase and beta-amylase.
- 8. The process of claim 7 further comprising saccharification of a starch hydrolysate of at least 30 percent by weight of dry solids.
- 9. The process of claim 7 in which the saccharification is conducted in the pH range of from 3.5 to 5.5 at a temperature in the range of from 55.degree. C. to 65.degree. C.
- 10. The process of claim 7 in which the dosages of glucoamylase and beta-amylase are in the range of from 0.05 to 0.5 AG units and from 0.005 to 0.3 beta-amylase units, respectively, per g of dry solids.
- 11. The process of claim 7 in which the dosage of debranching enzyme is in the range of from 0.005 to 5 pullulanase units per g of dry solids.
- 12. In a process for saccharifying a starch hydrolysate of about 30 percent to about 40 percent dry substance to a high DX dextrose syrup with glucoamylase, the improvement which comprises saccharifying at about 60.degree. C. or higher and pH in the range of about 4.0 to about 4.5 to 96 DX or higher by an enzyme mixture comprising a gluco-amylase dosage in the range of from 0.075 to 0.15 AG units per g D.S. and a dosage of the debranching enzyme product defined in claim 2 in the range of 1 to 2 pullulanase units per g D.S.
Parent Case Info
This is a continuation-in-part of Ser. No. 255,952, filed Apr. 20, 1981, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4011139 |
Horwath et al. |
Mar 1977 |
|
4211842 |
Marshall |
Jul 1980 |
|
Non-Patent Literature Citations (3)
Entry |
Ueda et al., Journal of Fermentation Technology, vol. 49, No. 6, pp. 552-558, (1971). |
Morgan et al., Journal of Applied Bacteriology, vol. 46, pp. 291-294, (1979). |
Bull, editor, Progress in Industrial Microbiology, vol. 15, pp. 127-150, (1979). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
255952 |
Apr 1981 |
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