Patent Application
20250011210
The invention relates to the field of degradation of fluor containing substances by bioconversion.
Per- and polyfluoroalkyl substances (PFAS) are highly stable molecules that contain multiple carbon-fluorine bonds (Shahsavari et al., 2021). They are used in various applications including non-stick coatings. Nowadays, PFAS are widespread in the environment. They have been detected in water bodies, household dust, soils and are likely to be present in foods (Shahsavari et al., 2021). Humans are exposed to PFAS through these food and non-food sources (Trudel et al., 2008) and their health can thereby be impacted for instance by affecting kidney, thyroid, or immune function (Shahsavari et al., 2021). Therefore, PFAS have to be removed from the environment.
Several approaches are used or are under study to remove PFAS from the environment (Shahsavari et al., 2021). At the moment, sorption to granular activated carbon (GAC) is the most used way to remove PFAS from drinking water and wastewater. GAC can be reactivated by treatment at high temperature (400-950° C.), thereby degrading the PFAS (DiStefano et al., 2022). However, this method is ineffective for short-chain PFAS removal and the amounts of GAC that are needed are very high (Shahsavari et al., 2021). Surface-Active Foam Fractionation (SAFF) has recently also been shown to be effective in removing PFAS from groundwater (Burns et al., 2021). More than 99.5% of PFOS, PFHxS, and PFOA was removed from contaminated groundwater. SAFF is sustainable since it does not require chemical reagents nor adsorbent media other than air introduced to the foam fractionation vessels. Air bubbles and GAC act both as adsorbents but air bubbles are cheap and do not require disposal after use. As with GAC, longer-chain PFAS species (benefiting from higher adsorption coefficients) are more easy to remove from contaminated water than short-chain species that are typified by lower adsorption coefficient values. Adding an anionic exchange (AIX) resin downstream of SAFF removes all trace detectable PFAS species.
Notably, both GAC and SAFF remove but do not degrade PFAS. An alternative for removal of PFAS is the use of microbes. Microbes can be used to bioaccumulate and biodegrade PFAS. Yet, PFAS is generally recalcitrant to degradation by microbes and the mechanisms of degradation are not known (Wei et al., 2019). Some bacteria (mainly identified as Pseudomonas species) can remove 28-67% of PFAS from contaminated soils in a period ranging between 2 and 100 days (Presentato et al., 2020; Yi et al., 2016; Kwon et al., 2014; Huang and Jaffe, 2019). It still has to be assessed whether the PFAS is (fully) degraded and which part is bioaccumulated. Only few studies have examined the ability of fungi to degrade PFAS. The white rot fungus Phanerochaete chrysosporium was shown to partly transform 6:2 FTOH, 8:2 FTOH and PFOS (Tseng et al., 2014; Merino 2016). For instance, 50% 6:2 FTOH and 70% 8:2 FTOH were transformed in 28 days into 5:3 polyfluorinated acid (40%), 5:2 FTOH (10%), PFHxA (4%) under laboratory conditions (Tseng et al., 2014; Merino et al., 2016). Highest activity was obtained when the medium was supplemented with lignocellulosic powder, yeast extract, cellulose, and glucose. The fungi Gloeophyllum trabeum and Trametes versicolor also biotransform 6:2 FTOH but at lower rates (Merino, 2016; Merino et al. 2018). The degradation products included 5:3 acid, 6:2 FTUCA, 5:2 ketone, 5:2 sFTOH, and PFHxA, which show that fungi can degrade 6:2 FTOH to other less fluorinated or more biodegradable metabolites. The articles describing fungal degradation of PFAS did not assess the underlying mechanism but other articles indicate that oxidative enzymes such as peroxidases and laccases are involved (Colosie et al., 2009; Luo et al., 2018; Huang, 2019). Moreover, changing medium composition and the presence of other microbes, as occurs in nature, may stimulate degradation of PFAS (Merino, 2016; Merino et al., 2018).
So, the state of the art methods to remove PFAS from contaminated soils are expensive, impractical for in situ treatment, use high pressure and temperatures, and/or result in toxic waste. Biodegradation by means of microbes has the potential to form the basis of a cost-effective, large scale in situ remediation strategy for PFAS. Literature (see above) has described the use of bioremediation to degrade, sorb and remove certain PFAS from water and soil. The main focus in literature is on bioremediation using bacteria. Only a few studies describe the use of mycoremediation (i.e. remediation with fungi) but success is limited, it involves long incubation times and often only uses one organism. Thus, so far biodegradation is a slow process, which makes it less attractable for industrial processes as waste water treatment
Accordingly, there is a need to improve processes to degrade PFAS.
The inventors have established a novel method of degradation of per- and polyfluoroalkyl substances (PFAS) by bioconversion.
Accordingly, there is provided a method for bioconversion of per- and polyfluoroalkyl substances (PFAS) comprising contacting a matter comprising the PFAS with an effective amount of a material colonized by edible mushroom forming fungi, and/or with an effective amount of an extract of a material colonized by edible mushroom forming fungi. Herein, this method is referred to as the “method according to the invention”, “the method”, or the “method of bioconversion”.
The term “bioconversion” is a process known to the person skilled in the art and is herein preferably construed as the conversion of a per- or polyfluoroalkyl substance (PFAS) into another compound by a biological process.
Per- and polyfluoroalkyl substances (PFAS) are known to the person skilled in the art and are herein preferably construed as a group of synthetic organofluorine chemical compounds that have multiple fluorine atoms attached to an alkyl chain. Herein below, a non exhaustive list of PFAS is set forward. The method of bioconversion comprises contacting the matter comprising the PFAS with the effective amount of a material colonized by edible mushroom forming fungi, and/or with the effective amount of an extract of a material colonized by edible mushroom forming fungi. Contacting may be performed using any method known in the art. The materials can e.g. be brought together in a container and mixed once, twice, multiple times or may be mixed continuously.
An effective amount is herein defined as an amount of the material colonized by edible mushroom forming fungi and/or an amount of the extract of a material colonized by edible mushroom forming fungi that mediates at least a measurable conversion of the PFAS in the matter comprising the PFAS.
In the embodiments herein, the matter comprising the PFAS may be any such matter known to the person skilled in the art. The matter may be a liquid or a non-liquid.
In the embodiments herein, the liquid may be any aqueous composition such as, but not limited to, an aqueous liquid such as water. Preferably, the liquid is groundwater, waste water, surface water or sub-surface water.
In the embodiments herein, the non-liquid may be a solid, such as but not limited to, soil or a composition comprising soil. A non-liquid such as soil may be completely dry or may contain some water. Preferably, the non-liquid is soil, earth or a material from a garbage dump.
In the embodiments herein, the PFAS may be any PFAS known to the person skilled in the art and may be grouped as set forward in: www.cdc.gov/exposurereport/pdf/Report_Chemical_List-508.pdf. PFAS typically consist of a fully (per) or partly (poly) fluorinated carbon chain connected to different functional groups. Based on the length of the fluorinated carbon chain, short and long chain PFAS can be distinguished. Long chains refer to:
A non-exhaustive preferred list of PFAS consists of: Perfluorobutanoic acid (PFBA), Perfluorobutane sulfonic acid (PFBS), Perfluorodecanoic acid (PFDA), Perfluoroheptane sulfonic acid (PFHpS), Perfluorododecanoic acid (PFDoA), Perfluoroheptanoic acid (PFHpA), Perfluorohexane sulfonic acid (PFHxS), Perfluorohexanoic acid (PFHxA), Perfluorononanoic acid (PFNA), Perfluorooctanoic acid (PFOA), n-Perfluorooctanoic acid (n-PFOA), Branched Perfluorooctanoic acid (Sb-PFOA), Perfluorooctane sulfonic acid (PFOS), n-Perfluorooctane sulfonic acid (n-PFOS), Branched Perfluoromethylheptane sulfonic acid (Sm-PFOS), Perfluorooctane sulfonamide (PFOSA or FOSA), Perfluoropentanoic acid (PFPeA), Perfluoroundecanoic acid (PUFA or PFUnDA), 2-(N-Ethyl-perfluorooctane sulfonamido) acetic acid (EtFOSAA), 2-(N-Methyl-perfluorooctane sulfonamido) acetic acid (Me-PFOSA-AcOH, or Me-FOSA-A), 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (9CI-PF3ONS), Adona (4,8-dioxa-3H-perfluorononanoate), and GenX (2,3,3,3-tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)-propanoic acid (HFPO-DA)).
Bioconversion of all PFAS listed herein functionally and structurally is within the scope of the claimed invention.
Preferably, in the embodiments herein, the PFAS is selected from the group consisting of: perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), perfluoro-n-butanoic acid (PFBA), perfluoro-n-pentanoic acid (PFPeA), perfluoro-n-hexanoic acid (PFHxA), perfluoro-n-butane sulfonate (PFBS), GenX (2,3,3,3-tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)-propanoic acid, and perfluoro-n-hexane sulfonate (PFHxS).
In the embodiment herein, the material colonized by edible mushroom forming fungi may be spent mushroom substrate (SMS) and/or casing layer and the extract of the material colonized by edible mushroom forming fungi may be tea extracted from SMS and/or from casing layer.
Accordingly, in the embodiments herein, the material colonized by edible mushroom forming fungi may be spent mushroom substrate (SMS), herein also depicted as spent mushroom compost, SMS compost, or spent mushroom substrate compost. The material colonized by edible mushroom forming fungi may be casing layer. The material colonized by edible mushroom forming fungi may be spent mushroom substrate (SMS) and casing layer. The extract of the material colonized by edible mushroom forming fungi may be tea extracted from spent mushroom substrate (SMS). The extract of the material colonized by edible mushroom forming fungi may be tea extracted from casing layer. The extract of the material colonized by edible mushroom forming fungi may be tea extracted from spent mushroom substrate (SMS) and from casing layer.
The material colonized by edible mushroom forming fungi may be any such material known to the person skilled in the art. Typically, such material comprises an agricultural substrate, which can be any material on or in which a fungus or fungal mycelium can grow, providing needs to the developing fungal mycelium or fungus. The substrate may comprise for instance one or more ingredients of the group consisting of soil, wood, wood chips or sawdust, straw, grain, wheat, manure (for instance straw-bedded horse or poultry manure), coffee grounds, waste or recycled paper, soybean meal, corncobs, nut or seed hulls, cocoa bean hulls, gypsum, cottonseed meal, ammonium nitrate, urea, and brewer's grain. The substrate may also comprise agro-industrial waste, produced from sectors such as the food industry and agriculture. Examples of agro-industrial waste are cellulose, hemicellulose, and lignin. The person skilled in the art knows how to choose the most suited substrate.
Spent mushroom substrate (SMS) is herein construed as the material of the agricultural substrate after fungal mycelium has been grown, optionally when mushrooms have grown and preferably after mushrooms, if present, have been removed from the material. SMS was long-time regarded as a waste stream but can be used for a variety of applications. Grimm and Wösten (2018) summarized such applications including the use to produce high-quality compost (Uzun 2004; Polat et al. 2009), use as substrate for production of other mushrooms (Stamets 1993), to feed animals and to improve their health (Song et al. 2007; Nasehi et al. 2017), to make biofuel production more effectively (Phan and Sabaratnam 2012), to produce materials (Jones et al. 2017; Islam et al. 2017; Appels et al. 2018), and to extract enzymes for industries and bioremediation (Phan and Sabaratnam 2012).
Spent mushroom substrate tea is herein construed as an aqueous extract of spent mushroom substrate. Tea may also be extracted from other material colonized by edible mushroom forming fungi, such as casing layer. Aqueous extract is herein construed as that water is mainly used to extract components from the spent mushroom substrate into a liquid, which is thus an aqueous liquid. The aqueous liquid that is incubated with the spent mushroom substrate may comprise other components in addition to water, such as a salt, a buffering agent and a detergent.
The term “fungal mycelium” or “mycelium” is herein defined as the vegetative part of a fungus, consisting of a network of branched, tubular filaments (hyphae) of fungi. A mycelium may be minute and forming a colony that is too small to see by eye, or it may be extensive. Mycelium allows a fungus to absorb water and nutrients from its environment. Mycelium makes up the thallus, or undifferentiated body, of a typical fungus and the mycelium may be microscopic in size and can develop into visible structures, such as rhizomorphs/cords (long strands of hyphae cemented together), or sclerotia (hard compact masses). A mycelium can form fruiting bodies such as brackets, mushrooms, and puffballs under the right environmental conditions and developmental state of the fungus.
The term “mushroom” is herein defined as the fleshy, spore-bearing fruiting body of a fungus, typically produced above ground on a substrate. A typical example of a “mushroom” is the cultivated white button mushroom, Agaricus bisporus, hence the word mushroom is most often applied to those fungi (Basidiomycota, Agaricomycetes) that have a stem (stipe), a cap (pileus), and gills (lamellae, sing. lamella) or pores on the underside of the cap. The word “mushroom” is also used for a wide variety of fungal fruiting bodies that produce sexual spores and that either or not have stems, and the term is used even more generally, to describe both the fleshy fruiting bodies of some Ascomycota and the woody or leathery fruiting bodies of some Basidiomycota. Mushroom forms deviating from the standard morphology usually have more specific names, such as “bracket”, “puffball”, “stinkhorn”, and “morel”, and gilled mushrooms themselves are often called “agarics” in reference to their similarity to Agaricus or their classification as Agaricales. By extension, the term “mushroom” can also designate the entire fungus when in culture or the thallus of species forming the fruiting bodies called mushrooms, or the species itself. Mushrooms have been widely used as foods and are grown on a commercial scale as edible mushrooms. Certain mushrooms are also used as medicine.
The term “spores” is herein defined as a unit of sexual or asexual fungal reproduction that contain all genetic material of a fungus, thereby allowing the fungus to spread and/or to survive.
The term “casing layer” is herein defined as a layer that is in contact with the substrate layer. It comprises moist material, either organic, inorganic, or a combination of the two, placed on top of the colonized substrate, with the purpose to help induce fruiting and to supply moisture to the substrate and the developing fruiting bodies. The skilled person will know how to select the appropriate casing material. The casing layer can be a nutritious or a non-nutritious layer made from materials such as compost, soil, peat moss, vermiculite, coconut coir, or different mixtures of the previous with various additives. The term “additives” is herein defined as mineral supplements, beneficial to the casing material, that supplies essential minerals for mushroom growth, or to balance the pH of the casing layer. The casing layer can be made with or without fungal mycelium.
If the casing layer comprises mycelium, it can be made using the Compost Added Casing (CACing) technique, which can accelerate casing colonization. This technique can involve the application of small amounts of compost fully colonized (spawn-run compost) by the mushroom mycelium to the casing layer.
In the embodiments herein, the edible mushroom is preferably a species of Agaricus, Pleurotus, Lentinula, Auricularia and Flammulina. Preferably, the species is selected from the group consisting of Agaricus bisporus, Pleurotus ostreatus, Lentinula edodes, Auricularia auricula-juda, and Flammulina velutipes. A most preferred species is Agaricus bisporus.
In the embodiments herein, the material colonized by edible mushroom forming fungi or extract thereof may comprise further fungi and/or prokaryotes. These further fungi and/or prokaryotes may be further fungi and/or prokaryotes that grow together with the mushroom forming fungus in the material. These further fungi and/or prokaryotes may also be added to the material colonized by edible mushroom forming fungi or extract thereof. A preferred fungus to be added is one selected from the group consisting of Mycothermus thermophilus, Phanerochaete species such as Phanerochaete chrysosporium, Gloeophyllum species, such as Gloeophyllum trabeum, Trametes species, such as Trametes versicolor, Aspergillus species, such as Aspergillus niger, Penicillium species, such as Penicillium chrysogenum, Fusarium species, Paecilomyces species, and Hypocrea species. Preferred prokaryotes are Mycobacterium species, such as Mycobacterium vaccae, Bacillus species, Pirrelula species, Thermus species and Pseudomonas species, such as Pseudomonas oleovoransh and Pseudomonas fluorescens.
The material colonized by edible mushroom forming fungi or extract thereof may further comprise microbes promoting directly or indirectly growth of the mushrooms and/or the mycelium in the substrate and/or are microbes preventing disease of the fungal mycelium and/or mushrooms. The microbes may have a beneficial effect on mycelium growth, mushroom growth, stimulate mushroom formation, prevent diseases or growth of pathogens. The skilled person will know what microbes to add to the substrate for promoting growth of mushrooms.
In the embodiments herein, the material colonized by edible mushroom forming fungi or extract thereof may further comprise compounds that can be beneficial for the bioconversion of PFAS. Such compounds may be, but are not limited to, nitrogen-containing molecules, calcium, copper, potassium, magnesium, sodium, iron, manganese, zinc, phosphate, and sulphate, and fungal enzymes such as peroxidases and laccases. Another such compound may be H2O2. These compounds may already be present in the material colonized by edible mushroom forming fungi or extract thereof, or may be added before or during the incubation with the matter comprising PFAS. In the embodiments herein, an enzyme may be added to the matter comprising the PFAS, to the material colonized by edible mushroom forming fungi or an extract thereof, and/or to the composition resulting from the contacting of the matter comprising the PFAS with the effective amount of a material colonized by edible mushroom forming fungi, and/or with the effective amount of an extract of a material colonized by edible mushroom forming fungi. Such enzyme preferably is a laccase and/or a peroxidase.
The incubation of the matter comprising the PFAS with the material colonized by edible mushroom forming fungi or an extract thereof may be performed at any suitable temperature, such as between about 4° C. and about 50° C. or between about 8° C. and about 45° C., such as at about 4° C., 5° C., 6° C., 7° C., 8° C., 9° C., 10° C., 11° C., 12° C., 13° C., 14° C., 15° C., 16° C., 17° C., 18° C., 19° C., 20° C., 21° C., 22° C., 23° C., 24° C., 25° C., 26° C., 27° C., 28° C., 29° C., 30° C., 31° C., 32° C., 33° C., 34° C., 35° C., 36° C., 37° C., 38° C., 39° C., 40° C., 41° C., 42° C., 43° C., 44° C., 45° C., 46° C., 47° C., 48° C., 49° C., or at about 50° C. In an embodiment, the incubation is performed at between 4° C. and 50° C. or between 8° C. and 45° C., such as at 4° C., 5° C., 6° C., 7° C., 8° C., 9° C., 10° C., 11° C., 12° C., 13° C., 14° C., 15° C., 16° C., 17° C., 18° C., 19° C., 20° C., 21° C., 22° C., 23° C., 24° C., 25° C., 26° C., 27° C., 28° C., 29° C., 30° C., 31° C., 32° C., 33° C., 34° C., 35° C., 36° C., 37° C., 38° C., 39° C., 40° C., 41° C., 42° C., 43° C., 44° C., 45° C., 46° C., 47° C., 48° C., 49° C., or at 50° C.
In the embodiments herein, preferably at least about 1% of the PFAS is converted, preferably into a less toxic or non-toxic compound. In the embodiments herein, at least about 1% is herein preferably at least about 1%, at least about 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 334%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 6%, 67%, 68%, 69%, 79%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 89%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, and most preferably at least about 99%.
In the embodiments herein, preferably at least 1% of the PFAS is converted, preferably into in a less toxic or non-toxic compound. In the embodiments herein, at least 1% is herein preferably at least 1%, at least 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 334%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 6%, 67%, 68%, 69%, 79%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 89%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, and most preferably at least 99%.
In the embodiments herein, the at least about 1% or the at least 1% PFAS is preferably converted in about 1 minute, 1 hour, 24 hours, one week, two weeks, three weeks, four weeks, one month, two months, three months, four months, five months, six months, seven months, eight months, nine months, ten months, eleven months, or in about one year.
In the embodiments herein, the at least about 1% or the at least 1% PFAS is preferably converted in 1 minute, 1 hour, 24 hours, one week, two weeks, three weeks, four weeks, one month, two months, three months, four months, five months, six months, seven months, eight months, nine months, ten months, eleven months, or in one year.
In a second aspect, there is provided a composition comprising the matter comprising the PFAS and further comprising the effective amount of a material colonized by edible mushroom forming fungi, and/or further comprising the effective amount of an extract of a material colonized by edible mushroom forming fungi. In this aspect, the features preferably are the features as set forward in the first aspect.
Preferably, the matter is a liquid or a non-liquid.
Preferably, the liquid is groundwater, waste water, surface water or subsurface water.
Preferably, the non-liquid is soil, earth or a material from a garbage dump.
Preferably, the PFAS is selected from the group consisting of: perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), perfluoro-n-butanoic acid (PFBA), perfluoro-n-pentanoic acid (PFPeA), perfluoro-n-hexanoic acid (PFHxA), perfluoro-n-butane sulfonate (PFBS), GenX (2,3,3,3-tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)-propanoic acid, and perfluoro-n-hexane sulfonate (PFHxS).
Preferably, the material colonized by edible mushroom forming fungi is spent mushroom substrate (SMS) and/or casing layer and the extract of the material colonized by edible mushroom forming fungi is tea extracted from spent mushroom substrate (SMS) and/or from casing layer.
Preferably, the material colonized by edible mushroom forming fungi or extract thereof comprises further fungi and/or prokaryotes.
Preferably, the composition further comprises an enzyme, which is added to the composition.
Preferably, the enzyme is a laccase and/or a peroxidase.
The composition may further comprise compounds that can be beneficial for the bioconversion of PFAS. Such compounds may be, but are not limited to, nitrogen-containing molecules, calcium, copper, potassium, magnesium, sodium, iron, manganese, zinc, phosphate, and sulphate, and fungal enzymes such as peroxidases and laccases. Another such compound may be H2O2. These compounds may already be present in the composition or in the material colonized by edible mushroom forming fungi or extract thereof, or may be added to the composition.
In a third aspect, there is provided for a device comprising the composition as defined in aspect 2 In this aspect, the features preferably are the features as set forward in the first and second aspect.
Such device is suitable for performing the method of the first aspect.
Preferably, the device is a container or a column.
In a fourth aspect, there is provided for a composition obtainable by the method of the first aspect. In this aspect, the features preferably are the features as set forward in the first, second and third aspect.
In this document and in its claims, the verb “to comprise” and its conjugations is used in its non-limiting sense to mean that items following the word are included, but items not specifically mentioned are not excluded. In addition the verb “to consist” may be replaced by “to consist essentially of” meaning that a product or a composition or a nucleic acid molecule or a peptide or polypeptide of a nucleic acid construct or vector or cell as defined herein may comprise additional component(s) than the ones specifically identified; said additional component(s) not altering the unique characteristic of the invention. In addition, reference to an element by the indefinite article “a” or “an” does not exclude the possibility that more than one of the elements is present, unless the context clearly requires that there be one and only one of the elements. The indefinite article “a” or “an” thus usually means “at least one”. The word “about” or “approximately” when used in association with a numerical value (e.g. about 10) preferably means that the value may be the given value (of 10) more or less 10% of the value.
All patent and literature references cited in the present specification are hereby incorporated by reference in their entirety.
Unless otherwise indicated each embodiment as described herein may be combined with another embodiment as described herein.
The examples herein are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way.
Further embodiments of the invention are listed here below.
The inventors have, in a non-limiting, exemplary, embodiment, used spent mushroom substrate (SMS) from the white button (Agaricus bisporus) industry. The SMS compost contains a microflora (bacteria and fungi; the latter being dominated by A. bisporus), chemical compounds such as nitrogen-containing molecules, calcium, copper, potassium, magnesium, sodium, iron, manganese, zinc, phosphate, and sulphate, and fungal enzymes such as peroxidases and laccases (see e.g. Gerrits, 1994, Straatsma et al, 2006, Bonnen et al, 1994, Thai et al, 2022). SMS was added to water spiked with 100-1000 μg L−1 GenX (also known as HFPO-DA or FRD-903) or PFOA in a volume to volume ratio of 1:20. The mix was incubated under static or shaken (175 rpm) conditions for 24 h at room temperature (21-25° C.). Sorption of PFAS was assessed with a methanol extraction of the SMS after incubation and washing with water. To determine removal over time, the mix was incubated for one week at 100 rpm and room temperature (21-25° C.). Next to mixing SMS with spiked water, removal of PFAS in time was measured by the use of SMS tea. Tea was made by shaking 10 g of SMS with 200 mL water for 1 h at 175 rpm. After filtering through a coffee filter, the tea was spiked with GenX and PFOA to a concentration of 100-1000 μg L−1. Alternatively, the tea was sterilized by heat treatment (121° C.) followed by filtration through a 0.22 μm filter, whereafter the sterilized tea was spiked with GenX and PFOA (100-1000 μg L−1). Samples from the different incubations were filtered using 0.22 μm centrifuge filters (Corning Costar Spin-X) and chromatographic separations were performed with an HPLC e2695 plus Autosampler (Waters Chromatography B.V.). A gradient of solvent A (deionized H2O (MiliQ) with 0.1% formic acid) and solvent B (methanol (HPLC, Biosolve) with 0.1% formic acid) was used for elution as follows: start with 50:50 (A:B), within 7 minutes linear to 15:85, hold this for 2 minutes, directly back to 50:50, 10 minutes. The gradient was performed at 35° C. at a flow rate of 0.8 mL min−1 using a X Bridge C18 3.5 μm column (100×4.6 mm), and a total running time of 10 minutes. GenX and PFOA were detected with a ACQUITY QDa Mass Detector (Waters Chromatopgraphy B.V.) with a negative polarity, capillary potential of 800 V and a source temperature of 450-600° C.
Results show that SMS removes 10-34% of GenX and 8-12% of PFOA after 1 day when incubated under static or shaken conditions (
With the use of SMS, we used a complex substrate which may not only use mycoremediation by A bisporus to remove PFAS. The bacterial community, other fungi as well as micro- and macro-nutrients in the compost may be involved as well. This combination of physio-chemical conditions and microbial degradation showed to be effective in the removal of several emerging contaminants, such as pesticides and pharmaceuticals (Lv et al., 2016; Liu et al., 2019). However, instead of using sequential treatments, for example GAC followed by chemical remediation, followed by microbial degradation, the use of SMS provides an all-in-one system. Despite the fact that SMS has been described previously (see e.g. Anton-Herrero et al., 2022; Corral-Bobadilla et al., 2019) to have bioremediation capacity it has never described to remove PFAS.
We conclude that:
Accordingly, we conclude that bioconversion of per- and polyfluoroalkyl (PFAS) is possible by contacting a matter comprising the PFAS with an effective amount of a material colonized by edible mushroom forming fungi, and/or with an effective amount of an extract of a material colonized by edible mushroom forming fungi.
| Number | Date | Country | Kind |
|---|---|---|---|
| 23183846.7 | Jul 2023 | EP | regional |
