Claims
- 1. An organized tissue, comprising:a plurality of substantially post-mitotic cells, wherein at least a subset of said cells form an organized tissue approximating the in vivo gross morphology of a tissue of interest, said subset of cells comprising cells having a foreign DNA sequence operably linked to a promoter and encoding a protein of a type or produced in an amount not produced normally by said tissue of interest, wherein the protein is produced at detectable levels in said organized tissue.
- 2. The organized tissue of claim 1, wherein said subset of cells comprise skeletal muscle.
- 3. An organized tissue attached to the surface of a substrate, comprising:a plurality of substantially post-mitotic cells, wherein at least a subset of said cells form an organized tissue approximating the in vivo gross morphology of a tissue of interest, said subset of cells comprising cells having a foreign DNA sequence operably linked to a promoter and encoding a protein of a type or produced in an amount not produced normally by said tissue of interest; and a substrate having first and second tissue attachment surfaces, wherein the organized tissue comprises a first end and a second end adherent to, respectively, said first and second tissue attachment surfaces and wherein tension is maintained between said first and second ends of said organized tissue such that upon detachment of said tissue from one of the tissue attachment surfaces said tissue undergoes a contraction.
- 4. The organized tissue attached to the surface of a substrate of claim 3 wherein upon detachment of said tissue from one of said tissue attachment surfaces and said contraction of said organized tissue, said tissue produces an amount of protein that is less than the amount produced when tension is maintained between said first and second ends of said organized tissue, wherein the protein is produced at detectable levels in said organized tissue.
- 5. The organized tissue attached to the surface of a substrate of claim 3, said substrate being selected from the group consisting of metal or plastic.
- 6. The organized tissue attached to the surface of a substrate of claim 5, said metal substrate being steel mesh having a longitudinal axis and first and second points for attachment, and wherein said first and second attachment sites of said tissue are attached, respectively, to said first and second ends of said organized tissue.
- 7. An organized tissue comprising a plurality of substantially post-mitotic cells, wherein at least a subset of cells form an organized tissue approximating the in vivo gross morphology of tissue of interest that is retained upon implantation of said tissue in to a mammal, said tissue producing a protein of a type or in an amount not normally produced by a tissue of interest, said subset of cells comprising a foreign DNA sequence operably linked to a promoter and encoding a protein, and wherein the organized tissue is further comprised of substantially post-mitotic cells, and wherein the protein is produced to detectable levels in said tissue.
- 8. An in vitro method for producing an organized tissue, comprising the steps of: providing cells of a tissue of interest, wherein at least a subset of said cells comprise a foreign DNA sequence operably linked to a promoter and encoding a protein, wherein said cells of said tissue of interest are mixed with an extracellular matrix to create a suspension;placing said suspension in a vessel having first and second tissue attachment surfaces thereon, wherein the tissue attachment surfaces are positioned such that as the tissue forms in vitro from said cells of said tissue of interest, said cells of said tissue of interest may adhere to said first and second attachment surfaces and align between said attachment surfaces and; allowing the suspension to coalesce; and culturing said coalesced suspension under conditions in which said organized tissue forms such that said cells form an organized tissue approximating the in vivo gross morphology of said tissue of interest and wherein the organized tissue forms such that a first end and a second end adheres to said tissue attachment surfaces, and wherein tension is maintained between the first and second ends of said organized tissue such that upon detachment of said tissue from one of the tissue attachment surfaces said tissue undergoes a contraction.
- 9. An in vivo method for producing a protein encoded by cells of an organized tissue, comprising a foreign DNA operably linked to a promoter and encoding said protein, comprising the steps of:providing cells of a tissue of interest, wherein at least a subset of said cells comprise a foreign DNA sequence operably linked to a promoter and encoding a protein, wherein said cells of said tissue of interest are mixed with an extracellular matrix to create a suspension; placing said suspension in a vessel having first and second tissue attachment surfaces thereon, wherein the tissue attachment surfaces are positioned such that as the tissue forms in vitro from said cells of said tissue of interest, said cells of said tissue of interest may adhere to said first and second attachment surfaces and align between said attachment surfaces and; allowing the suspension to coalesce; and culturing said coalesced suspension under conditions in which said organized tissue forms such that said cells form an organized tissue approximating the in vivo gross morphology of said tissue of interest and wherein the organized tissue forms such that a first end and a second end adheres to said tissue attachment surfaces, and wherein tension is maintained between the first and second ends of said organized tissue such that upon detachment of said tissue from one of the tissue attachment surfaces said tissue undergoes a contraction; and implanting said organized tissue into a mammal of the same species as said cells under conditions in which said protein encoded by said foreign DNA is produced.
- 10. The method of claim 8 or 9, wherein the step of providing comprises isolating primary cells of a cell type of said tissue of interest.
- 11. The method of claim 8 or 9, wherein the step of providing comprises providing immortalized cells of a cell type of said tissue of interest.
- 12. The method of claim 8 or 9, wherein said coalesced suspension exerts a force on said cells parallel to a dimension of said vessel.
- 13. The method of claim 8 or 9, wherein said cells are aligned parallel to a dimension of said vessel.
- 14. The method of claim 8 or 9, wherein said cells comprise skeletal muscle cells.
- 15. The method of claim 9, wherein said attachment surfaces are positioned at opposite ends of said vessel.
Parent Case Info
This application is a continuation-in-part of U.S. Ser. No. 08/712,111, filed Sep. 13, 1996, now U.S. Pat. No. 5,869,041, which is a continuation-in-part of U.S. Ser. No. 08/587,376, filed Jan. 12, 1996, now abandoned.
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Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
08/712111 |
Sep 1996 |
US |
Child |
08/896152 |
|
US |
Parent |
08/587376 |
Jan 1996 |
US |
Child |
08/712111 |
|
US |