Claims
- 1. A method of preparing deoxyribonucleotides in vitro, comprising the steps of:
a) preparing ribonucleotides extracted from yeast RNA; b) phosphorylating said yeast ribonucleotides to produce a phosphorylated ribonucleotide product; and c) converting said phosphorylated ribonucleotide product to deoxyribonucleotide product.
- 2. The method of claim 1, wherein said step c) was performed in a reaction solution containing a reducing agent and E. coli ribonucleotide reductase (RNR).
- 3. The method of claim 2, wherein said E. coli rebonucleotide reductase (RNR) is partially purified.
- 4. A deoxyribonucleotide product prepared in vitro by a method comprising the steps of:
a) preparing ribonucleotides extracted from yeast RNA; b) phosphorylating said yeast ribonucleotides to produce a phosphorylated ribonucleotide product; and c) converting said phosphorylated ribonucleotide product to deoxyribonucleotide product.
- 5. The method of claim 4, wherein the step c) was performed in a reaction solution containing a reducing agent and E. coli ribonucleotide reductase (RNR).
- 6. The method of claim 5, wherein said E. coli rebonucleotide reductase (RNR) is partially purified.
RELATED APPLICATIONS
[0001] This application claims priority from U.S. Provisional Patent Application Ser. No. 60/436,282 which was filed on Dec. 23, 2002, the content of which is hereby incorporated by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60436282 |
Dec 2002 |
US |