Derivatives of n-(arylsulfonyl)beta-aminoacids comprising a substituted aminomethyl group, the preparation method thereof and the pharmaceutical compositions containing same

[0001] The present invention relates to novel N-(arylsulphonyl)beta-amino acid derivatives comprising a substituted aminomethyl group, to their preparation and to pharmaceutical compositions containing them.

[0002] These compositions have affinity for the bradykinin (BK) receptors. Bradykinin is a nonapeptide belonging, like kallidin, to the kinin class and shows physiological activity in the cardiovascular field and as a mediator in inflammation and pain. Several bradykinin receptors are distinguished: the B1 and B2 receptors (D. Regoli et al., Pharmacol. Rev., 1980, 32, 1-46). More specifically, the B2 receptors are the receptors for bradykinin and kallidin: they are predominant and are especially found in most tissues; the B1 receptors are the receptors specific for [des-Arg9] bradykinin and [des-Arg10] kallidin: they are induced during inflammation processes.

[0003] Bradykinin receptors have been cloned for various species, especially for the human species: B1 receptor: J. G. Menke et al., J. Biol. Chem., 1994, 269 (34), 21583-21586; B2 receptor: J. F. Hess, Biochem. Biophys. Res. Commun., 1992, 184, 260-268.

[0004] Patent application WO 97/25315 describes compounds of formula:

[0005] in which:

[0006] RI, RII, RIII, RIV, RV, RVI, RVII, RVIII,RIX, RX and RXI have different values. These compounds show affinity for the bradykinin receptors.

[0007] Novel compounds have now been found, which show affinity for the bradykinin receptors with selectivity towards the bradykinin B1 receptors, which are bradykinin B1 receptor antagonists and which show advantages as regards their absorption.

[0008] These compounds may be used for the preparation of medicinal products that are useful for treating or preventing any pathology in which bradykinin and the B1 receptors are involved, especially inflammation pathologies and persistent or chronic inflammatory diseases.

[0009] Thus, according to one of its aspects, the subject of the present invention is compounds of formula:

[0010] in which:

[0011] R1 represents a phenylvinyl group; a phenyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a naphthyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a tetrahydronaphthyl group; a naphtho[2,3-d][1,3]dioxol-6-yl group; a heterocyclic radical chosen from quinolyl, isoquinolyl, 1-benzofur-2-yl, 2,1,3-benzoxadiazol-4-yl, 2,1,3-benzothiadiazol-4-yl, 1,3-benzothiazol-2-yl, 1-benzothiophen-2-yl, 1H-pyrazol-4-yl, thien-2-yl, 5-isoxazolthien-2-yl, benzothien-2-yl, thieno[3,2-c]pyrid-2-yl; the said heterocyclic radicals being unsubstituted or substituted one or more times with R6, which may be identical or different;

[0012] R2 represents hydrogen or a (C1-C4)alkyl group and R3 represents a phenyl group which is unsubstituted or substituted one or more times with R7, which may be identical or different; a heterocyclic radical chosen from benzo[1,3]dioxol-5-yl, 2,1,3-benzothiadiazol-5-yl, 2,1,3-benzoxadiazol-5-yl, benzothiophen-5-yl, 2,3-dihydrobenzo[1,4]dioxin-6-yl, benzofuryl, dihydrobenzofuryl, 1,3-thiazol-2-yl, furyl and thienyl, the said heterocyclic radical being unsubstituted or substituted one or more times with a halogen atom or with a (C1-C4)alkyl group;

[0013] or alternatively R2 represents a phenyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a heterocyclic radical chosen from benzo[1,3]dioxol-5-yl, pyridyl and indanyl, and R3 represents hydrogen;

[0014] R4 represents a group —CONR8R9; a group —CSNR8R9; a group —COR13; a phenyl group which is unsubstituted or substituted one or more times with R10; a heterocyclic radical chosen from pyridyl, imidazolyl, furyl, benzimidazolyl, benzothiazol-2-yl and benzo[1,3]dioxol-5-yl, the said radicals being unsubstituted or substituted with one or more methyl groups or halogen atoms;

[0015] R5 represents a group —CH2NR11R12 or —CH2N(O)NR11R12;

[0016] R6 represents a halogen atom; a (C1-C4)alkyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a 2-fluoroethoxy group; a trifluoromethoxy, methylenedioxy or difluoromethylenedioxy group;

[0017] R7 represents a halogen atom; a (C1-C4)alkyl group; a phenyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a benzyloxy group; a trifluoromethoxy group;

[0018] R8 and R9 each independently represent hydrogen; a (C1-C4)alkyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω(C1-C4)dialkylamino(C2-C4)alkyl group;

[0019] or alternatively R8 and R9, together with the nitrogen atom to which they are attached, constitute a heterocyclic radical chosen from pyrrolidinyl, morpholin-4-yl, thiomorpholin-4-yl, azepin-1-yl, piperidyl which is unsubstituted or substituted with one or more halogen atoms or one or more (C1-C4)alkyl or (C1-C4)alkoxy or trifluoromethyl groups, 3,4-dihydropiperid-1-yl, cyclohexyl-spiro-4-piperid-1-yl, and piperazinyl which is unsubstituted or substituted with one or more (C1-C4)alkyl groups;

[0020] R10 represents a halogen atom; a (C1-C4)alkyl group; a hydroxy group; a (C1-C6)alkoxy group; R10 can also represent a group —CH2NR11R12 when R5 represents a group —CH2NR11R12, the said groups then being identical;

[0021] R11 and R12 each independently represent hydrogen; a (C1-C6)alkyl group; a (C2-C4)alkenyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω-hydroxy(C2-C4)alkylene group; an ω-methoxy(C2-C4)alkylene group; ω-trifluoromethyl(C2-C4)alkylene group; an ω-halo(C2-C4)alkylene group,

[0022] or alternatively R11 and R12, together with the nitrogen atom to which they are attached, constitute a monocyclic, bicyclic or heterocyclic radical chosen from azetidinyl, pyrrolidinyl, morpholin-4-yl, thiomorpholin-4-yl, piperid-1-yl, piperazin-1-yl, 1,2,3,6-tetrahydropyrid-1-yl, 2,3,4,5-tetrahydropyridinium, decahydroquinolyl, decahydroisoquinolyl, tetrahydroisoquinolyl, octahydro-1H-isoindolyl, (C4-C6)cycloalkyl-spiro-piperidyl, 3-azabicyclo[3.1.0]hexyl and 7-azabicyclo[2.2.1]heptan-7-yl, which may be unsubstituted or substituted one or more times with a halogen atom or a (C1-C4)alkyl, hydroxyl, (C1-C4)alkoxy, trifluoromethyl, difluoromethylene or phenyl group;

[0023] R13 represents a phenyl, thiazol-2-yl or pyridyl group;

[0024] and also the salts thereof with mineral or organic acids and/or the solvates thereof or the hydrates thereof.

[0025] The term “halogen” means a fluorine, chlorine, bromine or iodine atom.

[0026] The terms “alkyl”, “alkylene” and “alkoxy”, respectively, mean a linear or branched alkyl radical, a linear or branched alkylene radical or a linear or branched alkoxy radical, respectively.

[0027] The compounds of formula (I) comprise at least one asymmetric carbon atom, and the pure enantiomers or diastereoisomers and also mixtures thereof in all proportions are subjects of the invention.

[0028] Preferably, a subject of the present invention is compounds of formula:

[0029] in which:

[0030] R1 represents a phenylvinyl group; a phenyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a naphthyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a tetrahydronaphthyl group; a heterocyclic radical chosen from quinolyl, 1-benzofur-2-yl, 2,1,3-benzoxadiazol-4-yl, 2,1,3-benzothiadiazol-4-yl, 1,3-benzothiazol-2-yl, 1-benzothiophen-2-yl, 1H-pyrazol-4-yl, thien-2-yl, 5-isoxazolethien-2-yl, benzothien-2-yl, thieno[3,2-c]pyrid-2-yl; naphtho[2,3-d][1,3]dioxol-6-yl; the said heterocyclic radicals being unsubstituted or substituted one or more times with R6, which may be identical or different;

[0031] R2 represents hydrogen or a (C1-C4)alkyl group and R3 represents a phenyl group which is unsubstituted or substituted one or more times with R7, which may be identical or different; a heterocyclic radical chosen from benzo[1,3]dioxol-5-yl which is unsubstituted or substituted in the −2 position with two fluorine atoms; 2,1,3-benzothiadiazol-5-yl; 2,3-dihydrobenzo[1,4]dioxin-6-yl; 1,3-thiazol-2-yl; 1-benzofur-2-yl; 1-benzofur-5-yl; furyl; thien-2-yl; thien-3-yl;

[0032] or R2 represents a phenyl group which is unsubstituted or substituted one or more times with R6, which may be identical or different; a heterocyclic radical chosen from benzo[1,3]dioxol-5-yl; pyridyl; indanyl; and R3 represents hydrogen;

[0033] R4 represents a group —CONR8R9; a phenyl group which is unsubstituted or substituted one or more times with R10; a heterocyclic radical chosen from pyridyl, imidazolyl, furyl, benzimidazolyl, benzothiazol-2-yl and benzo[1,3]dioxol-5-yl, the said radicals being unsubstituted or substituted with a methyl;

[0034] R5 represents a group —CH2NR11R12;

[0035] R6 represents a halogen atom; a (C1-C4)alkyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a 2-fluoroethoxy group; a trifluoromethoxy, methylenedioxy or difluoromethylenedioxy group;

[0036] R7 represents a halogen atom; a (C1-C4)alkyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a benzyloxy group; a trifluoromethoxy group;

[0037] R8 and R9 each independently represent hydrogen; a (C1-C4)alkyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω-(C1-C4)dialkylamino(C2-C4)alkyl group;

[0038] or R8 and R9, together with the nitrogen atom to which they are attached, constitute a heterocyclic radical chosen from pyrrolidinyl, piperidyl, morpholin-4-yl, thiomorpholin-4-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-methylpiperid-1-yl, 2-methylpiperid-1-yl, 4,4-dimethylpiperid-1-yl, 4,4-difluoropiperid-1-yl, 4-trifluoromethylpiperid-1-yl, 3,4-dihydropiperid-1-yl, azepin-1-yl and cyclohexyl-spiro-4-piperid-1-yl;

[0039] R10 represents a halogen atom; a (C1-C4)alkyl group; a hydroxyl group; a (C1-C6)alkoxy group; R10 can also represent a group -CH2NR11R12 when R5 represents a group -CH2NR11R12, the said groups then being identical;

[0040] R11 and R12 each independently represent hydrogen; a (C1-C6)alkyl group; a (C2-C4)alkenyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω-hydroxy(C2-C4)alkylene group; an ω-methoxy(C2-C4)alkylene group; an ω-trifluoromethyl(C2-C4)alkylene group; an ω-halo(C2-C4)alkylene group;

[0041] or R11 and R12, together with the nitrogen atom to which they are attached, constitute a monocyclic, bicyclic or heterocyclic radical chosen from azetidinyl, pyrrolidinyl, morpholin-4-yl, thiomorpholin-4-yl, piperid-1-yl, piperazin-1-yl, 1,2,3,6-tetrahydropyrid-1-yl, decahydroquinolyl, decahydroisoquinolyl, octahydro-1H-isoindolyl, (C4-C6)cycloalkyl-spiro-piperidyl and 3-azabicyclo[3.1.0]hexyl, which may be unsubstituted or substituted one or more times with a halogen atom or a (C1-C4)alkyl, hydroxyl, (C1-C4)alkoxy, trifluoromethyl, difluoromethylene or phenyl group;

[0042] and also the salts thereof with mineral or organic acids and/or solvates thereof or hydrates thereof.

[0043] Furthermore, certain values of the substituents are preferred. Thus, the compounds of formula (I) that are preferred are those in which at least one of the substituents has a value specified below:

[0044] a—R1 represents a 2,4-dichloro-3-methylphenyl, naphthyl, 6-methoxynaphth-2-yl, 3-methylbenzothiophen-2-yl, 3-methyl-5-chlorobenzothiophen-2-yl, 3-methyl-5-methoxybenzothiophen-2-yl, 3-methyl-6-methoxybenzothiophen-2-yl or 3-methyl-1-benzofur-2-yl group; R2, R3, R4 and R5 are as defined for a compound of formula (I);

[0045] b—R2 represents hydrogen and preferably R3 represents a benzo[1,3]dioxol-5-yl or phenyl group which is unsubstituted or substituted with a halogen; R1, R4 and R5 are as defined for a compound of formula (I);

[0046] c—R4 represents a group —CONR8R9 and preferably —NR8R9 represents a di(C1-C4)alkylamino radical, most particularly an N-methylisopropyl radical; a pyrrolidinyl or piperidyl group which is unsubstituted or substituted one or two times with a methyl or a halogen; R1, R2, R3 and R5 are as defined for a compound of formula (I);

[0047] d—R5 represents a group —CH2NR11R12 in which —NR11R12 represents an ethylisobutylamino, ethylisopropylamino, ethyl-tert-butylamino, diisopropylamino, cyclopentylmethylamino or cyclopentylethylamino radical or a piperidyl radical which is unsubstituted or substituted one or more times with a methyl or a halogen; R1, R2, R3 and R4 are as defined for a compound of formula (I).

[0048] Thus, preferably, the present invention relates to compounds of formula:

[0049] in which:

[0050] R1 represents a 2,4-dichloro-3-methylphenyl, naphthyl, 6-methoxynaphth-2-yl, 3-methylbenzothiophen-2-yl, 3-methyl-5-chlorobenzothiophen-2-yl, 3-methyl-5-methoxybenzothiophen-2-yl, 3-methyl-6-methoxybenzothiophen-2-yl or 3-methyl-1-benzofur-2-yl group;

[0051] R3 represents a benzo[1,3]dioxol-5-yl or phenyl group which is unsubstituted or substituted with a halogen;

[0052] R8 and R9, together with the nitrogen atom to which they are attached, constitute a di(C1-C4)alkylamino, pyrrolidinyl or piperidyl radical which is unsubstituted or substituted one or two times with a methyl or a halogen;

[0053] R11 and R12, together with the nitrogen atom to which they are attached, constitute an ethylisobutylamino, ethylisopropylamino, ethyl-tert-butylamino, diisopropylamino, cyclopentylmethylamino or cyclopentylethylamino radical or a piperidyl radical which is unsubstituted or substituted one or more times with a methyl or a halogen;

[0054] and also the salts thereof with mineral or organic acids and/or solvates thereof or hydrates thereof.

[0055] The compounds of formula (Ia) having the (R,R) configuration are particularly preferred.

[0056] Most particularly, a subject of the present invention is a compound chosen from:

[0057] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-(((6-methoxy-2-naphthyl)sulphonyl)amino)propanoyl)amino)-3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0058] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-((2-naphthylsulphonyl)amino)propanoyl)amino)-3-(4-((cyclopentyl(ethyl)amino)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0059] (R,R) 3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-2-((3-(((6-methoxy-2-naphthyl)sulphonyl)amino)-3-phenylpropanoyl)amino)-N-isopropyl-N-methyl propanamide;

[0060] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-(((6-methoxy-2-naphthyl)sulphonyl)amino)propanoyl)amino)-3-(4-((tert-butyl(ethyl)amino)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0061] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-(((3-methyl-1-benzothiophen-2-yl)sulphonyl)amino)propanoyl)amino)-3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0062] (R,R) 3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-2-((3-(((5-methoxy-3-methyl-1-benzothiophen-2-yl)sulphonyl)amino)-3-phenylpropanoyl)amino)-N-isopropyl-N-methylpropanamide;

[0063] (R,R) N-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)benzyl)-3-(((6-methoxy-2-naphthyl)sulphonyl)amino)-3-phenyl-N-(1-piperidylcarbonyl)propanamide;

[0064] (R,R) 2-((3-(4-chlorophenyl)-3-(((6-methoxy-2-naphthyl)sulphonyl)amino)propanoyl)amino)-3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0065] (R,R) 3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-2-((3-(((6-methoxy-2-naphthyl)sulphonyl)amino)-3-phenylpropanoyl)amino)-N,N-diethylpropanamide;

[0066] (R,R) 2-((3-(3-fluorophenyl)-3-(((6-methoxy-2-naphthyl)sulphonyl)amino)propanoyl)amino)-3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0067] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-(((3-methyl-1-benzofur-2-yl)sulphonyl)amino)propanoyl) 3-(4-((2,6-cis-dimethyl-1-piperidyl)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0068] (R,R) 2-((3-(1,3-benzodioxol-5-yl)-3-((2-naphthylsulphonyl)amino)propanoyl)amino)-3-(3-((tert-butyl(ethyl)amino)methyl)phenyl)-N-isopropyl-N-methylpropanamide;

[0069] (R,R) 3-(4-(7-azabicyclo[2.2.1]hept-7-ylmethyl)phenyl)-2-((3-(1,3-benzodioxol-5-yl)-3-(((6-methoxynaphthyl)sulphonyl)amino)propanoyl)amino)-N-isopropyl-N-methyl propanamide;

[0070] and also the salts thereof with mineral or organic acids and/or solvates thereof or hydrates thereof.

[0071] According to another of its aspects, the present invention relates to a process for preparing the compounds of formula (I), salts thereof and/or solvates thereof or hydrates thereof, characterized in that:

[0072] an acid or a functional derivative of this acid of formula:

[0073] in which R2 and R3 are as defined for a compound of formula (I), X represents either hydrogen or a group R1—SO2— in which R1 is as defined for a compound of formula (I), or an N-protecting group, is reacted with a compound of formula:

[0074] in which Y represents either R4 as defined for a compound of formula (I), or a (C1-C4)alkoxycarbonyl, and Z represents either R5 as defined for a compound of formula (I), or a —CN group, on the condition that, when Y represents R4 which represents a phenyl substituted with a group —CH2NR11R12, Z represents R5 which represents a group —CH2NR11R12, R11 and R12 being as defined for a compound of formula (I);

[0075] and

[0076] when X=R1SO2—, Y=R4 and Z=R5, the expected compound of formula (I) is obtained;

[0077] or when X≠R1SO2 and/or Y≠R4 and/or Z≠R5, that is to say when at least of the X, Y and Z groups represents, respectively, X═H or an N-protecting group, Y═(C1-C4)alkoxycarbonyl, Z=—CN, the compound thus obtained of formula:

[0078] is subjected to one or more of the following steps:

[0079] when X represents an N-protecting group, this group is removed and the compound thus obtained of formula:

[0080] is reacted with a sulphonyl halide of formula:

R1SO2-Hal (VI)

[0081] in which Hal represents a halogen;

[0082] when Y represents a (C1-C4)alkoxycarbonyl, it is hydrolyzed and the acid thus obtained or a functional derivative of this acid of formula:

[0083] is reacted with a compound of formula:

HNR8R9 (VIII);

[0084] in which R8 and R9 are as defined for a compound of formula (I);

[0085] when Z represents a CN group, this group is converted into R5.

[0086] Optionally, the compound thus obtained is converted into one of the salts thereof with mineral or organic acids.

[0087] In the course of any of the steps of the process for preparing the compounds of formula (I) or of the intermediate compounds thereof of formula (II), (III), (IV), (V) or (VII), it may be necessary and/or desirable to protect the reactive or sensitive functional groups, such as the amine, hydroxyl or carboxyl groups, present on any of the molecules concerned. This protection may be carried out using the conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, J. F. W. McOmie, Ed. Plenum Press, 1973 and in Protective Groups in Organic Synthesis, T. W. Greene and P. G. M. Wutts, published by John Wiley and Sons, 1991. The removal of the protecting groups may be carried out in a suitable subsequent step using the methods known to those skilled in the art, which do not affect the rest of the molecule concerned.

[0088] The N-protecting groups possibly used are the standard N-protecting groups that are well known to those skilled in the art, such as, for example, the tert-butoxycarbonyl, fluorenylmethoxycarbonyl, benzyl, trityl or benzyloxycarbonyl group.

[0089] The compounds of formula (I) or the compounds of formula (IV) may be prepared in the form of a mixture of isomers or in optically pure form. To obtain optically pure compounds, it is possible either to separate the isomers by known methods of organic chemistry, or to use optically pure compounds of formulae (II) and (III) as starting materials and then to perform, where appropriate, non-racemizing synthetic methods that are known per se.

[0090] In step a) of the process, the compounds of formula (I) or the compounds of formula (IV) are prepared using the standard methods of peptide chemistry, for example those described in The Peptides Ed. E. Gross and J. Meienhofer, Academic Press, 1979, 1, 65-104. Known methods make it possible to carry out peptide couplings without racemization of the carbon atoms of each constituent amino acid; furthermore, the β-substituted β-alanines for which the chiral carbon was not adjacent to the carboxyl group are renowned for not undergoing racemization (Ann. Rev. Biochem., 1986, 55, 855-878). Moreover, patent application EP 236 163 describes processes for conserving the chirality of each amino acid.

[0091] Thus, in step a) of the process according to the invention, the functional derivative of the acid (II) that may be used is a functional derivative that reacts with amines, such as an anhydride, a mixed anhydride, an acid chloride or an activated ester such as the 2,5-dioxopyrrolidin-1-yl ester, the p-nitrophenyl ester or the benzotriazol-1-yl ester.

[0092] When the 2,5-dioxopyrrolidin-1-yl ester of the acid (II) is used, the reaction with the amine (III) is carried out in a solvent such as N,N-dimethylformamide, in the presence of a base such as triethylamine or N-N-diisopropylthylamine, at a temperature of between 0° C. and room temperature.

[0093] When an acid chloride is used, the reaction is carried out in a solvent such as dichloromethane, in the presence of a base such as triethylamine, N-methylmorpholine or N,N-diisopropylthylamine, at a temperature of between −60° C. and room temperature.

[0094] When a mixed anhydride of the acid (II) is used, this anhydride is generated in situ by reacting a (C1-C4)alkyl chloroformate with the acid of formula (II) in a solvent such as dichloromethane, in the presence of a base such as triethylamine, at a temperature of between −70° C. and 50° C., and it is reacted with the amine of formula (III), in a solvent such as dichloromethane, in the presence of a base such as triethylamine and at a temperature of between 0° C. and room temperature.

[0095] When the acid of formula (II) itself is used, the process is performed in the presence of a coupling agent used in peptide chemistry, such as 1,3-dicyclohexylcarbodiimide or benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate or benzotriazol-1-yl-N,N,N′,N′-tetramethyluronium tetrafluoroborate in the presence of a base such as triethylamine, N,N-diisopropylethylamine or N-ethylmorpholine, in a solvent such as dichloromethane, acetonitrile or N,N-dimethylformamide, or a mixture of these solvents, at a temperature of between 0° C. and room temperature.

[0096] Either a compound of formula (I) is thus obtained directly, or a compound of formula (IV) is thus obtained in which at least one of the groups X, Y and Z represents, respectively: X=N-protecting group, Y=(C1-C4)alkoxycarbonyl, Z=—CN, which is converted in one or more reactions, by standard methods well known to those skilled in the art, into a compound of formula (I). It is understood that, when several reactions are needed to convert a compound of formula (IV) into a compound of formula (I), the order of these reactions is determined so as not to affect the other substituents of the molecule concerned.

[0097] When, in the compound of formula (IV), X represents an N-protecting group, this group is removed according to the methods known to those skilled in the art. For example, when X represents a tert-butoxycarbonyl group, it is removed by the action of an acid such as hydrochloric acid or trifluoroacetic acid, for example, in a solvent such as methanol, ethyl ether, dioxane, tetrahydrofuran or dichloromethane, at a temperature of between 0° C. and room temperature. Next, the amine of formula (V) thus obtained is reacted with a sulphonyl halide of formula (VI) (preferably a chloride), in the presence of a base such as an alkali metal hydroxide (for example sodium hydroxide or potassium hydroxide) or an organic base (for example triethylamine, diisopropylethylamine or N-methylmorpholine), in a solvent such as dioxane, dichloromethane or acetonitrile, in the presence or absence of an activator such as dimethylaminopyridine (DMAP); the reaction may also be carried out in pyridine in the presence or absence of DMAP. The reaction is carried out at a temperature of between 0° C. and room temperature.

[0098] Either a compound of formula (I) is obtained, or a compound of formula (IV) is obtained in which X=R1SO2—.

[0099] When, in the compound of formula (IV), Y represents a (C1-C4)alkoxycarbonyl, it is hydrolyzed in acidic or basic medium according to the methods known to those skilled in the art. Next, the acid of formula (VII) thus obtained is reacted with a compound of formula (VIII) under the conditions described previously for the use of a compound of formula (II). Either a compound of formula (I) is obtained, or a compound of formula (IV) is obtained in which R4=—CONR8R9.

[0100] When, in the compound of formula (IV), Z represents a cyano group, this group is converted into a group R5 according to the standard methods that are well known to those skilled in the art.

[0101] For example, reduction of the —CN group gives either a compound of formula (I) or a compound of formula (IV), in which R5=—CH2NH2. This reduction may be carried out using hydrogen, in the presence of a catalyst such as Raney® nickel, in a solvent such as methanol, toluene, dioxane or a mixture of these solvents, mixed with aqueous ammonia, at a temperature of between room temperature and 50° C.

[0102] The —CN group can be converted into a group R5=—CH2NR11R12 according to Scheme 1 below.

[0103] In step a1 of Scheme 1, the reduction of the nitrile derivative of formula (IV) to an aldehyde of formula (IX) is carried out according to the methods known to those skilled in the art such as, for example, the method described in Synth. Commun., 1990, 20(3), 459-467. The method described in Farmaco, Ed. Sci., 1988, 43(7/8), 597-612 may also be used, on the condition that, in the compound of formula (IV), X is other than an N-protecting group that is labile in acidic medium.

[0104] Next, in step b1, a compound of formula (X) is reacted with the aldehyde of formula (IX) in the presence or absence of an acid such as acetic acid, in a solvent such as methanol, dichloromethane or 1,2-dichloroethane, to form in situ an intermediate imine which is chemically reduced using, for example, sodium cyanoborohydride, sodium triacetoxyborohydride or sodium borohydride.

[0105] The —CN group can also be converted into a group R5 according to Scheme 2 below.

[0106] In step a2 of Scheme 2, the cyano group is reduced according to the methods described previously.

[0107] Next, in step b2, the amine thus obtained is converted into an alcohol of formula (XI) according to the methods known to those skilled in the art, for example by the action of sodium nitrite in aqueous medium, in a solvent such as dioxane and at a temperature of between room temperature and 110° C.

[0108] In step c2, the alcohol of formula (XI) is treated with methanesulphonyl chloride, in the presence of a base such as triethylamine, in a solvent such as dichloromethane and at a temperature of between 0° C. and the reflux point of the solvent. A compound of formula (XII) is thus obtained in which W=Cl or O—SO2CH3 depending on the operating conditions used.

[0109] In step d2, when a compound of formula (XII) in which W=Cl is used, the reaction is carried out with the compound of formula (X) in the presence of a base such as sodium hydride, in a solvent such as tetrahydrofuran, N,N-dimethylformamide, acetonitrile, toluene or 2-propanol and at a temperature of between room temperature and 100° C. When a compound of formula (XII) in which W=O—SO2CH3 is used, the reaction is carried out with a compound of formula (X) in the presence or absence of a base such as the triethylamine, in a solvent such as ethanol, N,N-dimethylformamide, acetonitrile or dichloromethane and at a temperature of between 0° C. and 100° C.

[0110] In step e2, the ester is reduced with a reducing agent such as NaBH4 or LiAlH4, for example.

[0111] A compound according to the invention of formula (I) in which R5 represents a group —CH2—N(O)—R11R12 is obtained from the analogous compound of formula (I) in which R5 represents a group —CH2—NR11R12, the other substituents being identical, and the reaction is carried out by the action of an oxidizing agent such as meta-chloroperbenzoic acid.

[0112] The compounds of formula (I) according to the invention are finally obtained.

[0113] The compounds of formula (I) thus obtained are isolated in the form of free base or in the form of salt, according to the standard techniques.

[0114] When the compounds of formula (I) are obtained in the form of free base, the salification is carried out by treatment with the chosen acid in an organic solvent. Treatment of the free base, dissolved, for example, in an ether such as diethyl ether or in an alcohol such as 2-propanol or methanol or in acetone or in dichloromethane or in ethyl acetate or in acetonitrile with a solution of the chosen acid in one of the abovementioned solvents, gives the corresponding salt, which is isolated according to the standard techniques.

[0115] Thus, the hydrochloride, the hydrobromide, the sulphate, the hydrogen sulphate, the dihydrogen phosphate, the methanesulphonate, the oxalate, the maleate, the succinate, the fumarate, the 2-naphthalenesulphonate, the benzenesulphonate or the para-toluenesulphonate, for example, is prepared.

[0116] At the end of the reaction, the compounds of formula (I) may be isolated in the form of one of the salts thereof, for example the chlorohydride; in this case, if it is necessary, the free base may be prepared by neutralizing the said salt with a mineral or organic base, such as sodium hydroxide or triethylamine or with an alkali metal carbonate or bicarbonate, such as sodium or potassium carbonate or bicarbonate.

[0117] The compounds of formula (VI) are known or prepared according to known methods. For example, 2,4-dichloro-3-methylbenzenesulphonyl chloride is prepared according to the process described in J. Am. Chem. Soc., 1940, 62, 511-512. 2-Quinolinesulphonyl chloride and 5,6,7,8-tetrahydronaphthalene-2-sulphonyl chloride are prepared according to the process described in WO 97/25315. 6-Methoxynaphthalene-2-sulphonyl chloride is prepared according to J. Org. Chem., 1992, 57, 2631-2641. 3-Methylbenzothienyl-2-sulphonyl chloride is prepared according to J. Het. Chem., 1988, 25, 639-641.

[0118] The compounds of formula (VIII) are known or prepared according to known methods.

[0119] The compounds of formula (X) are known or prepared according to known methods.

[0120] The compounds of formula (B), in which R2 represents hydrogen or a (C1-C4)alkyl in racemic form or in the form of pure enantiomers, are known (Table 1) or prepared according to known methods such as those described in WO 97/25315.

1TABLE I(II)

XR3ReferencesH

Eur. J. Med. Chem. Chim. Therap., 1992, 27 (9), 961-965Boc

J. Med. Chem., 1997, 40 (26), 4308-4318H

Bull. Soc. Chim. Fr., 1987, 6, 1079-1083H

Biorg. Med. Chem. 1994, 2 (9), 881H

Eur. J. Med. Chem. Chim. Therap., 1992, 27 (9), 961-965

Bull. Soc. Chim. Fr., 1987 6, 1079-1083H

Tetrahedron Lett., 1991, 32 (44), 6327-6328H

Zh. Obshch. Khim., 1958, 28, 213-215H

Chem. Heterocycl. Compd., (Engl. Transl.) 1969, 5, 453-456H

J. American. Chem. Soc., 1957, 79, 4524-4527H

Tetrahedron Lett., 2000, 41 (31), 5803-5806H

WO 97/08145H

WO 98/40055H

WO 97/08145H

Tetrahedron Lett. 2000, 41, 5803-5806

[0121] The compounds of formula (II) in which R3 represents hydrogen may be prepared by known methods. It is possible, for example, to use the process described in Scheme 3 below in which R′ represents a (C1-C4)alkyl and Pr represents an N-protecting group, for example a tert-butoxycarbonyl or fluorenylmethoxycarbonyl group.

[0122] In step a3 of Scheme 3, a compound of formula (XIII) is reacted with an acrylic acid ester of formula (XIV) to give a compound of formula (XV). The reaction is carried out in the presence of an acid such as acetic acid and at the reflux temperature of the reaction mixture.

[0123] In step b3, compound (XV) is reacted with a sulphonyl halide of formula (VI), in the presence of a base such as sodium hydroxide, potassium hydroxide or 4-dimethylaminopyridine, in a solvent such as dioxane or pyridine, and at a temperature of between room temperature and 60° C.

[0124] In step c3, the ester of formula (XVI) thus obtained is hydrolyzed, according to the methods known to those skilled in the art, to give the expected compound of formula (II) in which X=R1SO2—.

[0125] Alternatively, in step d3, the amino-ester of formula (XV) is protected according to the methods known to those skilled in the art, for example with a tert-butoxycarbonyl or fluorenylmethoxycarbonyl group.

[0126] In step e3, the ester of formula (XVII) thus obtained is hydrolyzed according to the known methods, to give the compound of formula (II) in which X represents a protecting group.

[0127] Alternatively, according to step f3, the ester of formula (XV) is hydrolyzed in acidic or basic medium to give a compound of formula (II).

[0128] The compounds of formula (III) in which Y represents a (C1-C4)alkoxycarbonyl or a group R4═CONR8R9 and Z=-CN are known or prepared according to known methods such as those described in WO 97/25315, EP 0 614 911 or EP 0 236 164.

[0129] The other compounds of formula (III) in which Y represents R4 and Z=-CN are prepared according to Scheme 4 below.

[0130] In step a4 of Scheme 4, the benzophenone imine is reacted with an amine of formula (XVIII) to give a compound of formula (XIX). The reaction is carried out in a solvent such as dichloromethane, chloroform or ethyl acetate, at a temperature of between room temperature and 50° C and in the presence or absence of a base such as triethylamine.

[0131] In step b4 the compound of formula (XIX) is treated with a strong base such as butyllithium, potassium tert-butoxide or lithium diisopropylamide to give a carbanion which is reacted with 4-(bromomethyl)benzonitrile.

[0132] The reaction is carried out in a solvent such as tetrahydrofuran, at a temperature of between −78° C. and room temperature. By treatment in acidic medium, the benzhydrylidene N-protecting group is removed to give an expected compound of formula (III).

[0133] Compounds of formula (XIX) may also be prepared according to the methods described in Bull. Soc. Chim. Fr., 1973, 2985, 2987, 2988, J. Am. Chem. Soc., 1982, 104 (3), 730 or Tetrahedron Lett., 1996, 1137.

[0134] The compounds of formula (XVIII) are known or prepared according to known methods. For example, compounds of formula (XVIII) may be prepared according to Scheme 5 below.

[0135] In step a5 of Scheme 5, a compound of formula (XX) is reacted with sodium azide to give a compound of formula (XXI). The reaction is carried out in a solvent such as dimethyl sulphoxide, at a temperature of between 0° C. and room temperature.

[0136] In step b5, the compound of formula (XXI) is reduced to a compound of formula (XVIII) according to the methods known to those skilled in the art.

[0137] The compounds of formula (XVIII) may also be prepared by reducing a nitrile of formula R4CN, for example by the action of LiAlH4.

[0138] The compounds of formula (III) in which Y represents a (C1-C4)alkoxycarbonyl and Z represents R5 as defined for a compound of formula (I) are prepared according to Scheme 6 below in which R′ represents a (C1-C4)alkyl.

[0139] In step a6 of Scheme 6, a compound of formula (XXII) is reacted with α,α′-dibromo-p-xylene (XXIII) according to the method described in Tetrahedron Asymmetry, 1992, 3 (5), 637-650.

[0140] In step b6, the compound of formula (XXIV) thus obtained is reacted with a compound of formula (X) to give a compound of formula (XXV). The reaction is carried out in the presence of a base such as an alkali metal carbonate or bicarbonate (potassium carbonate, sodium carbonate or sodium bicarbonate), in a solvent such as N,N-dimethylformamide, acetonitrile, dichloromethane or toluene and at a temperature of between room temperature and 100° C.

[0141] In step c6, the N-protecting group is removed by the action of an acid such as, for example, hydrochloric acid.

[0142] The compounds of formula (III) in which Y represents R4=CONR8R9 are also prepared according to Scheme 7 below in which R′ represents a (C1-C4)alkyl.

[0143] In step a7, the amine of a compound of formula (III) is protected with a tert-butoxycarbonyl group, and the ester thus obtained is then hydrolyzed according to the standard methods (step b7); the acid of formula (XXVII) thus obtained is then reacted with a compound of formula (VIII) according to the methods described previously (step c7) and the compound (XXVIII) obtained is deprotected in acidic medium (step d7).

[0144] The compounds of formula (III) in which Y represents R4 and Z represents R5 as defined for a compound of formula (I) are prepared according to Scheme 8 below.

[0145] In step a8 of Scheme 8, the compound of formula (XIX) is reacted with methyl 4-(bromomethyl)benzoate in the presence of a base such as potassium tert-butoxide or lithium diisopropylamide in a solvent such as tetrahydrofuran at a temperature of between −78° C. and room temperature. By treatment in acidic medium, the benzhydrylidene protecting group is removed.

[0146] In step b8, the amine of formula (XXIX) is protected by reaction with di-tert-butyl dicarbonate.

[0147] In step c8, the ester of formula (XXX) thus obtained is reduced to an alcohol of formula (XXXI). The reduction is carried out in the presence of an reducing agent such as lithium aluminium hydride, sodium borohydride or diisobutylaluminium hydride, in a solvent such as tetrahydrofuran or toluene, at a temperature of between −78° C. and room temperature.

[0148] The alcohol of formula (XXXI) is reacted in step d8 with methanesulphonyl chloride, in the presence of a base such as triethylamine, in a solvent such as dichloromethane or tetrahydrofuran and at a temperature of between 0° C. and the reflux point of the solvent.

[0149] In step e8, the compound of formula (XXXII) thus obtained is reacted with a compound of formula (X), in the presence or absence of a base such as triethylamine, in a solvent such as ethanol, N,N-dimethylformamide, acetonitrile, toluene or dichloromethane and at a temperature of between 0° C. and 100° C.

[0150] In step f8, the N-protecting group of the compound of formula (XXXIII) thus obtained is removed by treatment in acidic medium.

[0151] The compounds of formula (III) in which Y represents either a (C1-C4)alkoxycarbonyl, or R4 which represents a group —CONR8R9, a heterocyclic radical or a phenyl which is unsubstituted or substituted with a group other than a group —CH2NR11R12, and Z represents R5=—CH2NR11R12, may also be prepared according to Scheme 9 below:

[0152] In step a9 of Scheme 9, the amine of a compound of formula (III) is protected according to the conventional methods.

[0153] In step b9, the nitrile of formula (XXXIV) is reduced to an aldehyde of formula (XXXV) according to the method described in Synth. Commun., 1990, 20 (3), 459-467.

[0154] Next, in step c9, a compound of formula (X) is reacted with the aldehyde of formula (XXXV) in the presence or absence of an acid such as acetic acid, in a solvent such as methanol, dichloromethane or 1,2-dichloroethane to form in situ an imine intermediate which is chemically reduced using, for example, sodium cyanoborohydride, sodium triacetoxyborohydride or sodium borohydride.

[0155] Finally, in step d9, the N-protecting group of the compound of formula (XXXVI) is removed by treatment in acidic medium.

[0156] The amines of formula (X) are known or prepared according to known methods described below:

2TABLE IIHNR11R12References

J. Pharm. Sci., 1963, 52, 1191

J. Org. Chem., 1979, 44 (5), 771-7

Chem. Pharm. Bull., 1993, 41 (11), 1971-1986

WO 98/22443

J. Org. Chem., 1970, 35 (11), 3663-3666

Synth. Commun., 1999, 29 (10), 1747-1756

J. Chem. Soc. Perkin Trans. 2, 1984, 737-744

Patent US 2424063

J. Org. Chem. USSR (Engl. Trans 1), 1992, 28 (3.1), 374-380

Tetrahedron, 1999, 55 (31), 9439-9454

J. Amer. Chem. Soc., 1955, 77, 4100-4102

WO 95/22547

WO 94/03437

Chem. Ber., 1991, 791-801

[0157] A compound of formula (XXXV) may also be converted into a compound of formula (XXXVI) according to the following reaction scheme:

[0158] In step a9a, a reductive amination is carried out in the presence of a reducing agent such as NaHB(OAc)3. Next, either an alkylation is performed with an alkyl halide, for example an iodide (step b9a), or a further reductive amination is performed by reacting a carbonyl derivative of formula R12O (step c9a), R12O representing a dialkyl ketone or an alkylaldehyde. Thus, for example, treating a compound (XXXVa), in which R11 is cyclopropyl, with acetone gives a compound (XXXVI) in which R11 is cyclopropyl and R12 is isopropyl.

[0159] In the case where Y represents an alkoxycarbonyl group, a compound (III) in which Y represents a group CONR8R9 may be prepared according to Scheme 10 below:

[0160] The compounds of formula (III) in which Y represents a phenyl substituted with a group —CH2NR11R12 and Z represents R5═—CH2NR11R12 are prepared according to Scheme 11 below:

[0161] Steps a11 and b11 are carried out according to the methods described in Scheme 5.

[0162] Step c11 is carried out according to the process described in step a4 of Scheme 4.

[0163] Steps d11 to i11 are carried out according to the processes described in steps a8 to f8 of Scheme 8.

[0164] The compounds of formula (III) in which R4 represents a group COR13 may be prepared according to the scheme below.

[0165] In step a12, N,O-dimethylhydroxylamine hydrochloride is reacted in the presence of a coupling agent, and in step b11, the lithiated derivative of the compound R13H, prepared by the action of butyllithium on the compound R13H, is added. The process is then performed according to steps A, c9 and d9 of Scheme 9 to give a compound of formula (III) from the compound of formula (XXXXI).

[0166] The compounds of formula (III) in which R4 represents a group CSNR8R9 are prepared by the action of Lawesson's reagent on an analogous compound of formula (III) in which R4 represents a group CONR8R9 and the other substituents are identical (Tetrahedron, 1985, 41 (22), 5061-5087).

[0167] The optically pure compounds of formula (III) may be prepared by resolving the racemic mixtures by standard methods such as the method using optically active agents or enzymes. For example, when, in a compound of formula (III), Y represents a group —CONR8R9 or a (C1-C4)alkoxycarbonyl and Z=CN, the methods described in WO 97/25315 may be used; when, in a compound of formula (III), Y represents a pyridyl and Z=CN, the methods described in Tetrahedron, 1995, 51/46, 12731-12744 or in Synthesis, 1996, N° 8, 991-996 may be used.

[0168] Among the compounds of formulae (II) and (III), some are novel and constitute a further aspect of the invention.

[0169] The compounds of formula:

[0170] in which:

[0171] X represents hydrogen or an N-protecting group;

[0172] R3 represents a heterocyclic radical chosen from: (2,2-difluoro)benzo[1,3]dioxol-5-yl, 3-isopropylphenyl, 3-trifluoromethoxyphenyl, 2,1,3-benzoxadiazol-5-yl, benzothiophen-5-yl, 1-benzofur-6-yl, 1-benzofur-4-yl, 1-benzofur-3-methyl-5-yl, 2,3-dihydrobenzofur-4-yl;

[0173] and also the salts thereof with mineral or organic acids, in racemic form or in the form of pure enantiomers;

[0174] are novel and constitute a further aspect of the present invention.

[0175] The compounds of formula:

[0176] in which:

[0177] R1 represents a phenylvinyl; a heterocycle chosen from quinolyl, 1-benzofur-2-yl, 2,1,3-benzoxadiazol-4-yl, 2,1,3-benzothiadiazol-4-yl, 1,3-benzothiazol-2-yl, 1-benzothiophen-2-yl, 1H-pyrazol-4-yl, thien-2-yl, 5-isoxazolthien-2-yl, benzothien-2-yl, thieno[3,2-c]pyrid-2-yl; naphtho[2,3-d][1,3]dioxol-6-yl; the said heterocycles being unsubstituted or substituted one or more times with R6, which may be identical or different;

[0178] R2 represents hydrogen or a (C1-C4)alkyl and R3 represents a phenyl which is unsubstituted or substituted one or more times with R7, which may be identical or different; a heterocyclic radical chosen from benzo[1,3]dioxol-5-yl which is unsubstituted or substituted in position −2 with two fluorine atoms; 2,1,3-benzothiadiazol-5-yl; 2,1,3-benzoxadiazol-5-yl; benzothiophen-5-yl; 2,3-dihydro-benzo[1,4]dioxin-6-yl; 1-benzofur-2-yl; 1-benzofur-5-yl; 1-benzofur-6-yl; 1-benzofur-4-yl; 1-benzofur-3-methyl-5-yl; 2,3-dihydrobenzofur-4-yl; 1,3-thiazol-2-yl; furyl; thien-2-yl; thien-3-yl;

[0179] or R2 represents a phenyl which is unsubstituted or substituted one or more times with R6, which may be identical or different; a benzo[1,3]dioxol-5-yl; a pyridyl; an indanyl; and R3 represents hydrogen;

[0180] R6 represents a halogen atom; a (C1-C4)alkyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a 2-fluoroethoxy group; a trifluoromethoxy, methylenedioxy or difluoromethylenedioxy group;

[0181] R7 represents a halogen atom; a (C1-C4)alkyl group; a phenyl group; a trifluoromethyl group; a (C1-C4)alkoxy group; a benzyloxy group; a trifluoromethoxy group;

[0182] and also the salts thereof with mineral or organic acids, in racemic form or in the form of pure enantiomers, are novel and form part of the invention.

[0183] The compounds of formula:

[0184] in which:

[0185] R4 represents a group —CONR8R9; a group CSNR8R9; a group COR13; a phenyl which is unsubstituted or substituted one or more times with R10; a heterocyclic radical chosen from pyridyl, imidazolyl, furyl, benzimidazolyl, benzothiazol-2-yl and benzo[1,3]dioxol-5-yl, the said radicals being unsubstituted or substituted with a methyl;

[0186] R5 represents a group —CH2NR11R12 or —CH2N(O)NR11R12;

[0187] R8 and R9 each independently represent hydrogen; a (C1-C4)alkyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω(C1-C4)dialkylamino(C2-C4)alkyl group;

[0188] or R8 and R9, together with the nitrogen atom to which they are attached, constitute a heterocyclic radical chosen from pyrrolidinyl, piperidyl, morpholin-4-yl, thiomorpholin-4-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-methylpiperid-1-yl, 2-methylpiperid-1-yl, 4,4-dimethylpiperid-1-yl, 4,4-difluoropiperid-1-yl, 4-trifluoromethylpiperid-1-yl, 4-methoxypiperid-1-yl, 3,4-dihydropiperid-1-yl, azepin-1-yl and cyclohexyl-spiro-4-piperid-1-yl;

[0189] R10 represents a halogen atom; a (C1-C4)alkyl group; a hydroxyl group; a (C1-C6)alkoxy group; R10 can also represent a group —CH2NR11R12 when R5 represents a group —CH2NR11R12, the said groups then being identical;

[0190] R11 and R12 each independently represent hydrogen; a (C1-C6)alkyl group; a (C2-C4)alkenyl group; a (C3-C7)cycloalkyl group; a (C3-C7)cycloalkyl(C1-C4)alkyl group; an ω-hydroxy(C2-C4)alkylene group; an ω-methoxy(C2-C4)alkylene group; an ω-trifluoromethyl(C2-C4)alkylene group; an ω-halo(C2-C4)alkylene group;

[0191] or R11 and R12, together with the nitrogen atom to which they are attached, constitute a monocylic or bicyclic heterocyclic radical chosen from azetidinyl, pyrrolidinyl, morpholin-4-yl, thiomorpholin-4-yl, piperid-1-yl, piperazin-1-yl, 1,2,3,6-tetrahydropyrid-1-yl, 2,3,4,5-tetrahydropyridinium, decahydroquinolyl, decahydroisoquinolyl, tetrahydroisoquinolyl, octahydro-1H-isoindolyl, (C4-C6)cycloalkyl-spiro-piperidyl, 3-azabicyclo[3.1.0]hexyl and 7-azabicyclo[2.2.1]heptan-7-yl, which is unsubstituted or substituted one or more times with a halogen atom or a (C1-C4)alkyl, hydroxyl, (C1-C4)alkoxy, trifluoromethyl or difluoromethylene group;

[0192] R13 represents a phenyl, thiazol-2-yl or pyridyl group;

[0193] and also the salts thereof with mineral or organic acids, in the form of racemic mixtures or of pure enantiomers, are novel and form part of the invention.

[0194] The affinity of the compounds according to the invention for the bradykinin B1 receptors was measured on MRC5 cell membrane suspensions using a technique similar to the one described by K. H. Schneck et al., in Eur. J. Pharmacol., 1994, 266, 277-282. In this test, the affinity of [des-Arg9] bradykinin is between 10−6M and 10−7M, that of [des-Arg10]kallidin is 2×10−9M; and the compounds of the invention show affinity ranging up to 10−9M.

[0195] The affinity is expressed in terms of IC50, the IC50 being the concentration that inhibits 50% of the specific binding of the [des-Arg10]kallidin-tritiated ligand to the MRC5 cell receptors.

[0196] The affinity of the compounds according to the invention for the bradykinin B2 receptors was measured on MRC5 cell membrane suspensions according to a technique similar to the one described by D. G. Sawutz et al., in Eur. J. Pharmacol., 1992, 227, 309-315. In this test, the affinity of the bradykinin, expressed in terms of IC50, is in the region of 10−9 M, whereas the compounds of the invention show no affinity for the bradykinin B2 receptors at a concentration of 10−6 M.

[0197] The antagonistic effect of the compounds according to the invention was measured in vitro by inhibition of the contraction of rabbit thoracic aorta induced by the administration of [des-Arg9]bradykinin, after a preincubation of the tissue for 20 hours in Krebs buffer saturated with CO2/O2 mixture, according to an adaptation of the technique described by L. Levesque et al. Br. J. Pharmacol., 1993, 109, 1254-1262.

[0198] The antagonistic effect of the compounds according to the invention was also measured on the release of [3H]inositol phosphate by MRC5 fibroblasts in culture: after incorporation of [3H]myoinositol for 48 hours, according to the technique described by F. Oury Donat et al., J. Neurochem., 1994, 62, (4), 1399-1407. The antagonistic effect is expressed as the percentage of inhibition of the release of [3H]inositol phosphate induced by [des-Arg10]kallidin at 10−8 M, on MRC5 fibroblasts preincubated for 4 hours in the presence of IL1β at 0.5 μg/ml.

[0199] The intestinal absorption of the compounds according to the invention was studied in vitro on the model of CACO-2 cell monolayer, according to an adaptation of the technique described by T. Lindmark et al., J. Pharmacol. Exp. Therap., 1995, 275 (2), 958-964.

[0200] Moreover, several compounds according to the invention were studied in vivo on animal models.

[0201] The antinociceptive effect was checked on a model of neuropathic pain in rats after administration of a compound according to the invention at a dose of 30 mg/kg orally (according to the protocol described in Pain, 2000, 86 265-271).

[0202] It has been observed that a compound according to the invention, at a dose of from 1 to 30 mg/kg orally, inhibits the late phase of nociception induced with formalin in mice (Pain, 1987, 203-114); this is the sign of an action on inflammatory pain.

[0203] A model of thermal hyperalgia induced by UV irradiation in rats (Brit. Med. J., 1993, 110, 1441-1444) demonstrated the anti-hyperalgic effects of a compound according to the invention at a dose of from 1 to 3 mg/kg orally.

[0204] The compounds according to the invention may be useful for treating or preventing many pathologies, in particular inflammation pathologies, persistent or chronic inflammatory diseases (Drug News and Perspectives, 1994, 10 (7), 603-611), neurogenic inflammation, pain (Brit. J. Pharmacol., 1993, 110, 193-198), chronic pains, neuropathies, septic shock, burns (Pain, 1993, 53, 191-197), wounds, diseases of the respiratory pathways, asthma, systemic inflammatory response syndrome, oedema (Brit. J. Pharmacol., 1995, 114, 1005-1013), cerebral oedema, angiogenesis (Brit. J. Pharmacol., 1993, 109, 14-17), type I infectious diabetes (Abst. 14th Intern. Symp. on Kinins, C49, Denver Colo., 10-15 Sept. 1995), diabetic vasculopathy, ventricular hypertrophy, pulmonary fibrosis and systemic progressive sclerosis.

[0205] Mention may be made, for example, of:

[0206] inflammation, osteal pain, muscular pain, articular pain, facial pain, fibromyalgia, hyperalgia, pain associated with cancer, perioperative pain, menstrual pain, headaches, dental pain, gynaecological pain, migraines;

[0207] hyperactivity of the respiratory pathways in asthma, atopic or non-atopic asthma, allergic or non-allergic asthma, bronchitis, pneumoconiosis, chronic obstructive diseases of the respiratory pathways, pleurisy, chronic obstructive pulmonary diseases, rhinitis of viral or allergic origin;

[0208] post-capillary resistance, diabetes, diabetic vasculopathy, diabetic symptoms associated with insulitis (for example: hyperglycaemia, diuresis, proteinuria);

[0209] septic shock;

[0210] Alzheimer's disease, cranial trauma;

[0211] arthritis, rheumatoid arthritis, inflammatory diseases of the joints, atherosclerosis, multiple sclerosis;

[0212] diseases of the digestive system, diseases of the urinary system, cystitis, pancreatitis, nephritis, enterocolitis, ulcerative colitis, irritable bowel syndrome, Crohn's disease, liver diseases;

[0213] pathologies of the ocular system, uveitis, retinitis, glaucoma;

[0214] skin diseases such as atopic diseases, eczema, psoriasis, dermatitis, itching;

[0215] hair loss.

[0216] Moreover, the compounds of the invention are useful for their antiproliferative effect on cancer cells; their action on neurodegenerative diseases, myelin degeneration, degenerative diseases of viral origin; their cardioprotective effect.

[0217] Furthermore, the compounds of the invention are useful as myorelaxants, relaxants of smooth muscles, of spasms of the gastrointestinal tract and the uterus.

[0218] More generally, the compounds according to the invention may be useful for treating or preventing any pathology in which bradykinin plays a fundamental role, which are denoted hereinbelow as bradykinin-dependent pathologies.

[0219] The compounds of the present invention are especially active principles of pharmaceutical compositions, the toxicity of which is compatible with their use as medicinal products.

[0220] The compounds of formula (I) above may be used at daily doses of from 0.01 to 100 mg per kilo of body weight of the mammal to be treated, preferably at daily doses of from 0.1 to 50 mg/kg. In man, the dose may preferably range from 0.5 to 4000 mg per day and more particularly from 2.5 to 1000 mg depending on the age of the individual to be treated or the type of treatment: prophylactic or curative.

[0221] For their use as medicinal products, the compounds of formula (I) are generally administered in dosage units. The said dosage units are preferably formulated in pharmaceutical compositions in which the active principle is mixed with a pharmaceutical excipient.

[0222] Thus, according to another of its aspects, the present invention relates to pharmaceutical compositions containing, as active principle, a compound of formula (I) or a pharmaceutically acceptable salt and/or solvate or hydrate thereof.

[0223] In the pharmaceutical compositions of the present invention for oral, sublingual, inhaled, subcutaneous, intramuscular, intravenous, transdermal, local or rectal administration, the active principles may be administered in unit administration forms, as a mixture with standard pharmaceutical supports, to animals and to man. The appropriate unit administration forms comprise oral forms such as tablets, gel capsules, powders, granules and oral solutions or suspensions, sublingual and buccal administration forms, aerosols, topical administration forms, implants, subcutaneous, intramuscular, intravenous, intranasal or intraocular administration forms and rectal administration forms.

[0224] When a solid composition in the form of tablets is prepared, the active principle, micronized or non-micronized, is mixed with a pharmaceutical vehicle which may be composed of diluents such as, for example, lactose, microcrystalline cellulose, starch and formulation adjuvants, for instance binders (polyvinylpyrrolidone, hydroxypropylmethylcellulose, etc.), glidants, for instance silica, and lubricants, for instance magnesium stearate, stearic acid, glyceryl tribehenate or sodium stearylfumarate.

[0225] Wetting agents or surfactants such as sodium lauryl sulphate may be added to the formulation.

[0226] The tablets may be prepared by various techniques: direct tabletting, dry granulation, wet granulation or hot-melt granulation.

[0227] The tablets may be uncoated or sugar-coated (for example with sucrose) or coated with various polymers or other suitable materials.

[0228] The tablets may have a flash, delayed or sustained release by preparing polymer matrices or by using specific polymers in film coating.

[0229] A preparation as a gel capsule is obtained by simple mixing of the active principle with dry pharmaceutical vehicles (simple mixing or dry, wet or hot-melt granulation) or liquid or semi-solid pharmaceutical vehicles.

[0230] The gel capsules may be soft or hard, and film-coated or otherwise, so as to have a flash, sustained or delayed activity (for example via an enteric form).

[0231] A preparation in the form of a syrup or elixir may contain the active principle together with a sweetener, preferably a calorie-free sweetener, methylparaben and propylparaben as antiseptic, and also a flavouring and a suitable dye.

[0232] The water-dispersible powders or granules may contain the active principle as a mixture with dispersants, wetting agents or suspending agents, for instance polyvinylpyrrolidone, and also with sweeteners or flavour enhancers.

[0233] For rectal administration, use is made of suppositories which are prepared with binders that melt at the rectal temperature, for example cocoa butter or polyethylene glycols.

[0234] Aqueous suspensions, isotonic saline solutions or sterile injectable solutions containing pharmacologically acceptable dispersants and/or solubilizing agents, for example propylene glycol or butylene glycol, are used for parenteral, intranasal or intraocular administration.

[0235] Thus, to prepare an aqueous solution for intravenous injection, a cosolvent such as, for example, an alcohol such as ethanol or a glycol such as polyethylene glycol or propylene glycol, and a hydrophilic surfactant such as Tween® 80, may be used. To prepare an oily solution for intramuscular injection, the active principle may be dissolved with a triglyceride or a glycerol ester.

[0236] Creams, ointments, gels or eye drops may be used for local administration.

[0237] Patches in multilayer or reservoir form in which the active principle may be in an alcoholic solution may be used for transdermal administration.

[0238] For administration by inhalation, an aerosol containing, for example, sorbitan trioleate or oleic acid and also trichlorofluoromethane, dichlorofluoromethane, dichlorotetrafluoroethane or any other biologically compatible propellent gas is used; a system containing the active principle alone or combined with an excipient, in powder form, may also be used.

[0239] The active principle may also be in complex form with a cyclodextrin, for example α, β or γ-cyclodextrin, 2-hydroxypropyl-β-cyclodextrin or methyl-β-cyclodextrin.

[0240] The active principle may also be formulated in the form of microcapsules or microspheres, optionally with one or more supports or additives.

[0241] Among the sustained-release forms that are useful in the case of chronic treatment, implants may be used. These may be prepared in the form of an oily suspension or in the form of a suspension of microspheres in an isotonic medium.

[0242] In each dosage unit, the active principle of formula (I) is present in amounts that are adapted to the daily doses envisaged. In general, each dosage unit is suitably adjusted according to the dosage and the intended type of administration, for example tablets, gel capsules and the like, sachets, ampules, syrups and the like, or drops, such that such a dosage unit contains from 0.5 to 1000 mg of active principle and preferably from 2.5 to 250 mg needing to be administered one to four times a day.

[0243] The compositions of the present invention may contain, along with the compounds of formula (I) above or a pharmaceutically acceptable salt and/or solvate or hydrate thereof, other active principles which may be useful in the treatment of the complaints or diseases mentioned above. For example, a pharmaceutical composition according to the present invention may contain a bradykinin B2 receptor antagonist combined with a compound according to the present invention.

[0244] According to another of its aspects, the present invention relates to the use of the compounds of formula (I), or a pharmaceutically acceptable salt and/or solvate or hydrate thereof, for the preparation of medicinal products intended for treating any pathology in which bradykinin and B1 receptors are involved.

[0245] According to another of its aspects, the present invention relates to the use of the compounds of formula (I), or a pharmaceutically acceptable salt and/or solvate or hydrate thereof, for the preparation of medicinal products intended for treating inflammation pathologies and persistent or chronic inflammatory diseases.

[0246] In the Preparations and the Examples, the following abbreviations are used:

[0247] ether: diethyl ether

[0248] iso ether: diisopropyl ether

[0249] DCM: dichloromethane

[0250] DMF: N,N-dimethylformamide

[0251] DMSO: dimethyl sulphoxide

[0252] EtOAc: ethyl acetate

[0253] THF: tetrahydrofaran

[0254] AcOH: acetic acid

[0255] TFA: trifluoroacetic acid

[0256] TEA: triethylamine

[0257] DIPEA: diisopropylethylamine

[0258] DMAP: 4-dimethylaminopyridine

[0259] DCE: dichloroethane

[0260] DCC: 1,3-dicyclohexylcarbodiimide

[0261] DCU: dicyclohexylurea

[0262] TBTU: O-(1H-benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate

[0263] Boc: tert-butoxycarbonyl

[0264] (Boc)2O: di-tert-butyl dicarbonate

[0265] Penicillin amidase sold by Sigma

[0266] Alcalase sold by Novo

[0267] Sephadex® LH 20: sold by Pharmacia

[0268] BOP: benzotriazol-1-yloxytris(dimethylamino) phosphonium hexafluorophosphate

[0269] TBTU: benzotriazol-1-yl-N,N,N′,N′-tetramethyluronium tetrafluoroborate

[0270] KHSO4/K2SO4 buffer: solution of 16.66 g of KHSO4 and 32.32 g of K2SO4 in 1 litre of water

[0271] Hydrochloric ether: saturated solution of HCl gas in diethyl ether

[0272] m.p.: melting point

[0273] RT: room temperature

[0274] Except where otherwise mentioned, the proton nuclear magnetic resonance (NMR) spectra are recorded at 200 MHz in DMSO-d6 optionally containing TFA, and the reference is placed on the DMSO which is at 2.50 ppm from tetramethylsilane. The chemical shifts δ are indicated in ppm.

[0275] s: singlet; bs: broad singlet; ds: doubled singlet; d: doublet; bd: broad doublet; dd: doubled doublet: t: triplet; q: quartet; qt: quintet; mt: multiplet; unres.: unresolved peak; sept.: septet.

[0276] When only the NMR indication appears in the present description, this means that the NMR spectrum recorded under the above conditions is in accordance with the expected structure.

[0277] The mass spectra indicate the value MH+.

PREPARATIONS

Preparation 1.1

3
-(Naphthalene-2-sulphonylamino)-3-phenylpropionic acid

[0278]

[0279] 4.13 g of 3-amino-3-phenylpropionic acid are dissolved in a mixture of 100 ml of dioxane and 25 ml of 1N NaOH, 5.6 g of 2-naphthalenesulphonyl chloride are added portionwise while maintaining a pH of 10.5-10.8 by addition of 1N NaOH, and the mixture is stirred for 2 hours at RT. The reaction mixture is diluted by adding 400 ml of water, acidified to pH 2 by adding 2N HCl, and extracted with EtOAc, the organic phase is washed with KHSO4/K2SO4 buffer solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 7.33 g of the expected product are obtained after triturating in heptane and drying under vacuum; m.p.=126-129° C.

[0280] NMR: δ (ppm): 2.55-2.70: mt: 2H; 4.70: t: 1H; 6.85-8.15: unres.: 12H.

Preparation 1.2

3-[Methyl(naphthalene-2-sulphonyl)amino]-3-phenylpropionic acid.

[0281]

A) 3-(tert-Butoxycarbonylamino)-3-phenylpropionic acid

[0282] 8.3 ml of triethylamine are added to a mixture of 8.3 g of 3-amino-3-phenylpropionic acid in 35 ml of water and 5 ml of dioxane, followed by addition, over 30 minutes, of a solution of 12.8 g of di-tert-butyl dicarbonate in 25 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is diluted with water, the aqueous phase is washed with ether and acidified to pH 2.5 by adding 2N HCl, and extracted with EtOAc, the organic phase is washed with water, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 12.4 g of the expected product are obtained.

B) 3-(N-tert-Butoxycarbonylmethylamino)-3-phenylpropionic acid

[0283] 0.27 g of 80% sodium hydride in oil is added portionwise to a mixture of 0.795 g of the compound obtained in the preceding step and 0.62 ml of methyl iodide in 10 ml of THF, and the mixture is stirred overnight at RT. The reaction mixture is diluted with EtOAc, water is added and the mixture is acidified to pH 2 by adding 1N HCl. After separation of the phases by settling, the organic phase is washed with water, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.82 g of the expected product is obtained.

C) 3-(Methylamino)-3-phenylpropionyl trifluoroacetate

[0284] A mixture of 0.81 g of the compound obtained in the preceding step and 12 ml of TFA in 10 ml of DCM is stirred for 55 minutes at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in DCM and the solvent is evaporated off under vacuum. The residue is dissolved in ether, heptane is added to the point of precipitation, the solvent is decanted off and the gum obtained is dried. 0.86 g of the expected product is obtained in the form of a foam.

D) 3-[Methyl(naphthalene-2-sulphonyl)amino]-3-phenylpropionic acid

[0285] 5 ml of 1N NaOH are added to a mixture of 0.85 g of the compound obtained in the preceding step in 5 ml of dioxane, followed by portionwise addition of 0.698 g of 2-naphthalenesulphonyl chloride, while keeping the pH at 10-11 by adding 1N NaOH, and the mixture is stirred for 2 hours at RT. The reaction mixture is diluted with water, the aqueous phase is washed with ether and acidified to pH 2 by adding 1N HCl, and extracted with EtOAc, the organic phase is washed with water, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.65 g of the expected product is obtained in the form of a wax.

Preparation 1.3

3-(3-Methylphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0286]

A) 3-Amino-3-(3-methylphenyl)propionic acid

[0287] 11.8 ml of 3-methylbenzaldehyde are added to a mixture of 10.4 g of malonic acid and 15.4 g of ammonium acetate in 150 ml of 2-methoxyethanol, and the mixture is heated overnight at 80° C. After cooling to RT, the precipitate formed is filtered off under suction, washed with ether and dried. 6.8 g of the expected product are obtained.

[0288] NMR: δ (ppm): 2.25: s: 3H; 2.80 to 3.05: mt: 2H; 4,55: t: 1H; 7.10 to 7.30: unres.: 4H.

B) 3-(3-Methylphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0289] 10 ml of 1N NaOH are added to a suspension of 1.79 g of the compound obtained in the preceding step in 25 ml of dioxane, followed by portionwise addition of 2.26 g of 2-naphthalenesulphonyl chloride, while keeping the pH at 10.5-12 by adding 1N NaOH, and the mixture is stirred for 2 hours at RT. The reaction mixture is diluted with water, the aqueous phase is washed with EtOAc and acidified to pH 1 by adding 6N HCl, and extracted with EtOAc, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 3.37 g of the expected product are obtained after crystallization from heptane.

Preparation 1.4

3-(3,4-Dimethylphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0290]

A) 3-Amino-3-(3,4-dimethylphenyl)propionic acid hydrochloride

[0291] A mixture of 5 g of 3,4-dimethylbenzaldehyde, 3.88 g of malonic acid and 5.74 g of ammonium acetate in 50 ml of EtOH is refluxed for 5 hours. After cooling to RT, the precipitate formed is filtered off by suction and washed with EtOH. The precipitate is taken up in a DCM/1N HCl mixture, the insoluble material is filtered off, the filtrate is decanted and the acidic aqueous phase is concentrated under vacuum up to the start of precipitation and cooled to 0° C., and the precipitate formed is filtered off by suction. The precipitate is taken up in a DCM/MeOH mixture (6/4; v/v) and dried over Na2SO4, and the solvents are evaporated off under vacuum. The residue is taken up in ether and the precipitate formed is filtered off by suction after trituration. 3.01 g of the expected product are obtained; m.p.=192° C. (dec.).

B) 3-(3,4-Dimethylphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0292] A solution of 1.98 g of 2-naphthalenesulphonyl chloride in 15 ml of dioxane is added dropwise to a mixture of 2 g of the compound obtained in the preceding step and 17.4 ml of 1N NaOH in 20 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is neutralized to pH 7 by adding 1N HCl and concentrated under vacuum. The residue is taken up in an EtOAc/saturated NaHCO3 mixture, the precipitate formed is filtered off by suction and taken up in a DCM/1N HCl mixture, the organic phase is dried over Na2SO4 after separation of the phases by settling, and the solvent is evaporated off under vacuum. 1.66 g of the expected product are obtained; m.p.=122° C. (dec.).

Preparation 1.5

3-(3,5-Dimethoxyphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0293]

A) 3-Amino-3-(3,5-dimethoxyphenyl)propionic acid

[0294] A mixture of 5 g of 3,5-dimethoxybenzaldehyde, 3.13 g of malonic acid and 4.64 g of ammonium acetate in 50 ml of EtOH is refluxed for 5 hours. The reaction mixture is concentrated under vacuum, the residue is taken up in water, insoluble material is filtered off, the filtrate is washed with DCM and the aqueous phase is concentrated under vacuum. The residue is taken up in water and concentrated under vacuum again. The residue is taken up in EtOH and the solvent is evaporated off under vacuum. 2.96 g of the expected product are obtained.

B) 3-(3,5-Dimethoxyphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0295] 1.01 g of 2-naphthalenesulphonyl chloride are added portionwise to a mixture of 1 g of the compound obtained in the preceding step and 4.5 ml of 1N NaOH in 15 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is diluted with water and washed with EtOAc, the aqueous phase is acidified to pH 1 by adding concentrated HCl, and extracted with ether, the organic phase is washed with 1N HCl and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.41 g of the expected product are obtained; m.p.=190° C.

Preparation 1.6

3-(3,4-Dimethoxyphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0296]

A) 3-Amino-3-(3,4-dimethoxyphenyl)propionic acid, hydrochloride

[0297] A mixture of 5 g of 3,4-dimethoxybenzaldehyde, 3.131 g of malonic acid and 4.64 g of ammonium acetate in 50 ml of EtOH is refluxed for 5 hours. After cooling to RT, the precipitate formed is filtered off by suction. The precipitate is taken up in water, acidified to pH 2 by adding 1N HCl, and the aqueous phase is washed with DCM and concentrated under vacuum. The residue is taken up in water and concentrated again under vacuum. The residue is taken up in EtOH and evaporated under vacuum. 2.99 g of the expected product are obtained.

B) 3-(3,4-Dimethoxyphenyl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0298] This compound is prepared according to the procedure described in step B) of preparation 1.5, starting with 1 g of the compound obtained in the preceding step and 7.65 ml of 1N NaOH in 15 ml of dioxane. 1.33 g of the expected product are obtained.

Preparation 1.7

3-(Benzo [1,3 ]dioxol-5-yl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0299]

A) 3-Amino-3-(benzo[1,3]dioxol-5-yl)propionic acid

[0300] This compound is prepared according to the procedure described in Biorg. Med. Chem., 1994, 2 (9), 881.

B) 3-(Benzo[1,3]dioxol-5-yl)-3-(naphthalene-2-sulphonylamino)propionic acid

[0301] 1N NaOH is added to a suspension of 2.16 g of the compound obtained in the preceding step in 40 ml of dioxane, until the pH=11.8, followed by portionwise addition of 2.33 g of 2-naphthalenesulphonyl chloride, and the mixture is stirred for 2 hours at RT while keeping the pH at 10.5-11.5 by adding 1N NaOH. The reaction mixture is diluted with an equal volume of water and washed with EtOAc, the aqueous phase is acidified to pH 1.5 by adding 6N HCl, and extracted with EtOAc, the organic phase is washed with KHSO4/K2SO4 buffer, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 3.9 g of the expected product are obtained after crystallization from heptane; m.p.=173-175° C.

[0302] NMR: δ (ppm): 2.45-2.60: mt: 2H; 4.65: q: 1H; 5.40-5.70: mt: 2H; 6.45-6.65: mt: 3H; 7.55-7.75: mt: 3H; 7.90-8.10: mt: 4H; 8.35: bd: 1H.

Preparation 1.8

3-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-3-(naphthalene-2-sulphonylamino) propionic acid

[0303]

A) 3-Amino-3-(2,3-dihydrobenzo[1,4]dioxin-6-yl)propionic acid

[0304] A mixture of 5 g of 2,3-dihydrobenzo[1,4]dioxine-6-carbaldehyde, 3.17 g of malonic acid and 4.69 g of ammonium acetate in 50 ml of EtOH is refluxed for 5 hours. The reaction mixture is allowed to cool to RT and the precipitate formed is filtered off by suction and washed with EtOH and then with water. 1.965 g of the expected product are obtained after drying under vacuum.

B) 3-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-3-(naphthalene-2-sulphonylamino) propionic acid

[0305] This compound is prepared according to the procedure described in step B of preparation 1.5, starting with 1 g of the compound obtained in the preceding step, 4.5 ml of 1N NaOH, 15 ml of dioxane and 1.02 g of 2-naphthalenesulphonyl chloride. 0.876 g of the expected product is obtained after crystallization from hexane.

Preparation 1.9

3-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-3-(5,6,7,8-tetrahydronaphthalene-2-sulphonylamino)propionic acid

[0306]

[0307] A solution of 0.570 g of 5,6,7,8-tetrahydronaphthalene-2-sulphonyl chloride in 15 ml of dioxane is added dropwise to a mixture of 0.5 g of the compound obtained in step A of preparation 1.8 and 4.5 ml of 1N NaOH in 20 ml of dioxane, and the mixture is stirred for 4 hours at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in 1N NaOH and washed with DCM, the aqueous phase is acidified to pH 1 by adding concentrated HCl, and extracted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.5 g of the expected product is obtained.

Preparation 1.10

3-[(2,4-Dichloro-3-methylbenzenesulphonyl)phenylamino]propionic acid

[0308]

A) 2,4-Dichloro-3-methylbenzenesulphonyl chloride

[0309] This compound is prepared according to the procedure described in J. Am. Chem. Soc., 1940, 62, 511-512.

B) Methyl 3-(phenylamino)propionate

[0310] A mixture of 20 ml of aniline, 22 ml of methyl acrylate and 2 ml of acetic acid is refluxed for 8 hours. After concentrating the reaction mixture under vacuum, the resulting oil is distilled off under reduced pressure (b.p.=132° C. at 333.3 Pa and then b.p.=110° C. at 6.66 Pa). The product obtained is taken up in hexane and the precipitate formed is filtered off by suction. 25 g of the expected product are obtained.

C) Methyl 3-[(2,4-dichloro-3-methylbenzenesulphonyl)phenylamino]propionate

[0311] A mixture of 1.5 g of the compound obtained in step A, 1.03 g of the compound obtained in step B and 0.08 g of DMAP in 20 ml of pyridine is stirred for 1 hour at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with a buffer solution pH=2, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2.32 g of the expected product are obtained.

D) 3-[(2,4-Dichloro-3-methylbenzenesulphonyl)phenylamino]propionic acid

[0312] 8.7 ml of 1N KOH are added to a solution of 2.32 g of the compound obtained in the preceding step in 10 ml of EtOH and 10 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in saturated NaHCO3 solution, the aqueous phase is washed with ether, acidified to pH 1 by adding 1N HCl and extracted with DCM, the organic phase is washed with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.912 g of the expected product is obtained.

[0313] NMR: δ (ppm): 2.35: t: 2H; 2.5: s: 3H; 4.0: t: 2H; 7.15 to 7.4: unres.: 5H; 7.45 7.65: q: 2H; 12.2 to 12.5: bs: 1H.

Preparation 1.11

2,5-Dioxopyrrolidin-1-yl 3-(naphthalene-2-sulphonylamino)-3-phenylpropionate

[0314] 1.13 g of 1,3-dicyclohexylcarbodiimide are added to a mixture of 1.78 g of the compound obtained in Preparation 1.1 and 0.578 g of N-hydroxysuccinimide in 15 ml of DMF, and the mixture is stirred overnight at RT. After filtering off by suction the 1,3-dicyclohexylurea formed, the filtrate is diluted with water and extracted with EtOAc, the organic phase is washed with saturated NaHCO3 solution, with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.81 g of the expected product are obtained.

Preparation 1.12

3-Phenyl-3-(quinoline-2-sulphonylamino)propionic acid

[0315]

[0316] This compound is prepared according to the procedure described in Preparation 3.13 of international patent application WO 97/25315.

Preparation 1.13

(R) 3-(N-Boc)Amino-3-(benzo[1.3]dioxol-5-yl)propionic acid

[0317]

A) (R) 3-(Phenylacetyl)amino-3-(benzo[1,3]dioxol-5-yl)propionic acid

[0318] 20 g of 3-amino-3-(benzo[1,3]dioxol-5-yl) propionic acid hydrochloride are placed in 10 ml of acetone, 30 ml of water and 38 ml of TEA at −5° C. and 14 ml of phenylacetyl chloride in 20 ml of acetone are added dropwise, followed by stirring for 2 hours at −5° C. The acetone is concentrated. The aqueous phase is washed with Et2O and the insoluble material is removed by filtration. The phases are separated again by settling and the aqueous phase is washed with Et2O and then acidified with 1N HCl to pH 2. The resulting phase is poured into DCM and a precipitate forms. The precipitate is filtered off by suction and rinsed with DCM. 24 g of the expected compound are obtained.

B) (R) 3-Amino-3-(benzo[1,3]dioxol-5-yl)propionic acid HCl

[0319] 4 g of the compound from the preceding step are placed in 150 ml of water, 12.23 ml of 1N KOH are added and the mixture is stirred for 15 minutes at RT. The pH of the solution is approximately 11; AcOH is added and the pH is adjusted to 7.5±0.1. 250 μl of penicillin amidase are added and the mixture is stirred overnight at RT, while maintaining the pH at 7.5±0.1. The mixture is acidified to pH 2 by adding 1N HCl and then washed with EtOAc. The aqueous phase is heated to 65° C. with active vegetable charcoal for 5 minutes. The resulting mixture is filtered through Celite® and the aqueous phase is washed with Et2O and then concentrated to dryness and evaporated. The residue is taken up in an MeOH/DCM mixture (6/4; v/v) and the insoluble material (mineral) is then removed and the organic phase is dried over sodium sulphate and concentrated. 1.75 g of expected compound are obtained.

[0320] NMR: δ (ppm): 2.7-3.1: mt: 2H; 4.4: unres.: 1H; 6.0: s: 2H; 6.9: q: 2H; 7.1: s: 1H.

C) (R) 3-(N-Boc)Amino-3-(benzo[1.3]dioxol-5-yl)propionic acid

[0321] 1.30 g of the compound obtained in the preceding step are placed in 20 ml of dioxane and 20 ml of water; 1.8 ml of TEA and then 1.54 g of (Boc)2O are added and the mixture is stirred overnight at RT. The reaction mixture is diluted with water, washed with Et2O and then acidified to pH 2 by addition of KHSO4/K2SO4. The resulting mixture is extracted with EtOAc and then washed with saturated NaCl solution. 0.850 g of the expected compound is obtained.

αd25×+54°(c=0.5; MeOH)

[0322] By working according to the methods described above, the compounds of formula (II) described in Table III below are prepared:

3TABLE III(II)

PreparationXR2R3m.p. ° C.1.14

1.15

1.16

1.17

H174° C.1.18

139-142° C.1.19

148° C.1.20

H120° C.1.21

185° C.1.22

H159° C.1.23

68° C.1.24

Hoil1.25TFA

H137° C.1.26TFA

H184° C.1.27TFA

H163° C.1.28

100

H180° C.1.29TFA

101

102

H154° C.1.30

103

104

H160° C.1.31

105

106

H193° C.

Preparation 1.14

[0323] NMR: 2.2 ppm: mt: 2H; 2.8 ppm: s: 6H; 4.5 ppm: t: 1H; 6.9 ppm: mt: 6H; 7.2 ppm: d: 1H; 7.4 ppm: t: 1H; 7.55 ppm: t: 1H; 7.9 ppm: d: 1H; 8.3 ppm: t: 1H.

Preparation 1.15

[0324] NMR; 2.55 ppm: mt: 2H; 4.65 ppm: mt: 1H; 6.4-8.0 ppm: mt: 15 H; 8.1 ppm: s: 1H; 8.4 ppm: d: 1H.

Preparation 1.16

[0325] NMR; 2.5-2.7 ppm: mt: 2H; 4.7 ppm: q: 1H; 7.0-8.1 ppm: mt: 11H; 8.5 ppm: d: 1H.

Preparation 1.17

[0326] NMR (DMSO+TFA): 2.4ppm: t: 2H; 2.55ppm: s: 3H; 4.0 ppm: t: 2H; 6.05 ppm: s: 2H; 6.6 ppm: dd: 1H; 6.8 ppm: mt: 2H; 7.55 ppm: d: 1H; 7.65 ppm: d: 1H.

Preparation 1.18

[0327] NMR (DMSO+TFA): 2.40-2.65 ppm: mt: 2H; 4.60-4.75 ppm: t: 1H; 6.20 ppm: s: 1H; 7.10-8.40 ppm: unres.: 9H.

Preparation 1.25

[0328] NMR (250 MHz): 2.35-2.45 ppm: t: 2H; 3.80-3.90 ppm: t: 2H; 7.30-8.60 ppm: mt: 11H.

Preparation 1.26

[0329] NMR (250 MHz): 2.35-2.45 ppm: t: 2H; 2.50 ppm: s: 3H; 3.95-4.05 ppm: t: 2H; 7.35-8.50 ppm: mt: 6H.

Preparation 1.27

[0330] NMR (250 MHz): 2.40 ppm: s: 3H; 2.50-2.60 ppm: t: 2H; 4.15-4.25 ppm: t: 2H; 7.55-8.65 ppm: mt: 6H.

Preparation 1.28

[0331] NMR: 2.55-2.75 ppm: t: 2H; 4.15-4.45 ppm: t: 2H; 7.55-8.75 ppm: mt: 11H.

Preparation 1.29

[0332] NMR (DMSO+TFA): 2.4 ppm: t: 2H; 2.5 ppm: mt: 5H; 4.0 ppm: t: 2H; 7.25 ppm: dd: 1H; 7.7 ppm: mt: 4H.

Preparation 1.30

[0333] NMR: 2.4 ppm: t: 2H; 2.5 ppm: mt: 5H; 4.0 ppm: unres.: 2H; 7.3-7.6 ppm: mt: 6H.

Preparation 1.31

[0334] NMR (DMSO+TFA): 1.9 ppm: quint: 2H; 2.25 ppm: t: 2H; 2.4 ppm: mt: 4H; 2.85 ppm: t: 4H; 3.85 ppm: t: 2H; 6.8: dd: 1H; 7.0 ppm: unres.: 2H; 7.5 ppm: q: 2H.

Preparation 1.32

[0335]

107

A) S-(4-Methoxy)phenyl ethanethioate

[0336] 15 g of 4-methoxybenzenethiol are dissolved in 20 ml of water containing 4.3 g of NaOH and the mixture is stirred for 20 minutes at RT. 8.5 ml of 1-chloroacetone are added dropwise and the mixture is stirred for 1 hour at RT. The reaction mixture is extracted with ether (twice), dried and evaporated to give 20 g of the expected compound.

B) 5-Methoxy-3-methylbenzothiophene

[0337] 10 g of the compound from the preceding step and 20 ml of polyphosphoric acid are mixed together in 400 ml of chlorobenzene, and the mixture is stirred for 1 hour at RT and then heated at 120° C. for 18 hours. The reaction mixture is allowed to cool and the phases are then separated by settling. The residual oil is taken up in DCM (twice) and the phases are then separated again by settling.

[0338] The supernatant phases are combined and then evaporated. The residue is taken up in DCM and then washed with water, followed by NaHCO3 solution. After evaporating off the solvent, the residue is chromatographed on silica, eluting with a hexane/EtOAc mixture (98/2; v/v) to give 5.2 g of the expected compound.

C) Lithium (5-methoxy-3-methyl-1-benzothiophen-2-yl)sulphinate

[0339] 1.69 g of the compound from the preceding step are dissolved in 10 ml of THF and the mixture is cooled to −20° C. 6.6 ml of butyllithium (1.8M in hexane) are added over 15 minutes, followed by slow addition of SO2 in an amount sufficient to saturate the medium. After stirring for 20 minutes at −20° C., the mixture is allowed to warm to RT. 50 ml of Et2O are added and the resulting mixture is then filtered by suction and dried to give 2.4 g of the expected compound.

D) 5-Methoxy-3-methyl-1-benzothiophene-2-sulphonyl chloride

[0340] 2.4 g of the compound from the preceding step are suspended in 20 ml of CH2Cl2. The mixture is cooled to 5° C., followed by portionwise addition of 1.26 g of N-chlorosuccinimide, and the resulting mixture is stirred for one hour at 5° C. The reaction mixture is washed with water and the water is re-extracted with CH2Cl2. The organic phases are combined, washed with NaCl solution, dried and evaporated to give 1.8 g of the expected compound.

[0341] NMR: 2.50 ppm: s: 3H; 3.85 ppm: s: 3H; 7.0-7.80 ppm: mt: 3H.

E) The process is then performed according to the usual methods to obtain the expected compound

Preparation 1.33

[0342]

108

A) 6-Methoxy-3-methyl-1-benzothiophene-2-sulphonyl chloride

[0343] This compound is prepared according to the process described in the above preparation.

[0344] NMR: 2.50 ppm: s: 3H; 3.85 ppm: s: 3H; 7.0-7.70 ppm: mt: 3H.

B)

[0345] The process is then performed according to the usual methods to obtain the expected compound.

[0346] Other compounds of formula (II) prepared according to the methods known from the literature or described above were prepared in racemic form or in the form of pure isomers.

4TABLE IV(II)

109

Preparationm.p. ° C. or(isomer)XR3NMR1.34Boc

110

114° C.1.35

111

112

159° C.1.36

113

114

NMR1.37

115

116

248° C. 145° C.1.38 (R)Boc

117

120° C.1.39H Boc

118

NMR 167° C.1.40 (R)H

119

NMR1.41H Boc

120

242° C. 190° C.1.42H Boc

121

260° C. 198° C.1.43H Boc

122

228° C. NMR1.44H Boc

123

204° C. 125° C.1.45 (a)H Boc

124

NMR NMR

[0347] (a) This compound is prepared from 1-benzofurancarbonitrile described in European patent application 540 041.

Preparation 2.1

2-Amino-3-(4-cyanophenyl)-1-(pyrrolidin-1-yl)propan-1-one trifluoroacetate

[0348]

125

[0349] 7.5 g of BOP are added to a mixture of 4.06 g of 2-(tert-butoxycarbonylamino)-3-(4-cyanophenyl) propionic acid and 1.15 ml of pyrrolidine in 20 ml of DMF, and the mixture is stirred for 2 hours at RT, while keeping the pH at 7 by adding N-ethylmorpholine. The reaction mixture is concentrated under vacuum and the residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (95/5; v/v). The product obtained is taken up in 20 ml of TFA in 20 ml of DCM, and the reaction mixture is stirred for 30 minutes at RT and concentrated under vacuum. The residue is taken up in ether and the precipitate formed is filtered off by suction. 3.95 g of the expected product are obtained after drying.

Preparation 2.2

2-Amino-3-[4-(tert-butylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one bis(trifluoroacetate)

[0350]

126

A) Ethyl 2-(benzhydrylideneamino)-3-(4-bromomethylphenyl)propionate

[0351] This compound is prepared according to the procedure described in Tetrahedron: Asymmetry, 1992, 3 (5), 637-650.

B) Ethyl 2-(benzhydrylideneamino)-3-[4-(tert-butylaminomethyl)phenyl]propionate

[0352] 0.76 g of K2CO3 is added to a solution of 0.58 ml of tert-butylamine in 8 ml of DMF, and the mixture is stirred for 20 minutes at RT. A solution of 2.15 g of the compound obtained in the preceding step in 3 ml of DMF is then added and the mixture is stirred for 3 hours 30 minutes at RT. The reaction mixture is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2.67 g of the expected product are obtained in the form of an oil.

C) Ethyl 2-amino-3-[4-(tert-butylaminomethyl)phenyl]propionate

[0353] A mixture of 2.66 g of the compound obtained in the preceding step and 30 ml of aqueous 1N HCl solution in 30 ml of ether is stirred overnight at RT. After separation of the phases by settling, EtOAc is added to the acidic aqueous phase and the mixture is basified to pH 11 by adding 10N NaOH. After separation of the phases by settling, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.88 g of the expected product is obtained in the form of an oil.

D) Ethyl 2-(tert-butoxycarbonylamino)-3-[4-(tert-butylaminomethyl)phenyl]propionate

[0354] 0.7 g of di-tert-butyl dicarbonate is added portionwise to a solution of 0.88 g of the compound obtained in the preceding step in 12 ml of dioxane, and the mixture is stirred for 1 hour 30 minutes at RT. The reaction mixture is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.34 g of the expected product are obtained in the form of an oil.

E) 2-(tert-Butoxycarbonylamino)-3-[4-(tert-butylaminomethyl)phenyl]propionic acid

[0355] A mixture of 1.33 g of the compound obtained in the preceding step and 0.75 ml of 8.3N KOH in 8 ml of MeOH is stirred for 2 hours at RT. A chloroform/water mixture is added to the reaction mixture and the resulting mixture is acidified to pH 4 by adding 10N HCl. After separation of the phases by settling, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum to give a first crop of 0.54 g of the expected product. The aqueous phases and the washing liquors are concentrated under vacuum, the residue is taken up in 2-propanol, the insoluble material is filtered off and the filtrate is concentrated under vacuum to give a second crop of 0.51 g of the expected product.

F) 2-(tert-Butoxycarbonylamino)-3-[4-(tert-butylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one

[0356] 0.6 ml of DIPEA is added to a mixture of 1.03 g of the compound obtained in the preceding step in 15 ml of DMF, followed by addition of 0.28 ml of pyrrolidine and 1.46 g of BOP, and the mixture is stirred for 4 hours at RT, while maintaining the pH at 6 by adding DIPEA. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with 0.2N NaOH, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.3 g of the expected product are obtained in the form of an oil.

G) 2-Amino-3-[4-(tert-butylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one bis(trifluoroacetate)

[0357] A mixture of 1.29 g of the compound obtained in the preceding step and 17 ml of TFA in 15 ml of DCM is stirred for 50 minutes at RT. After concentrating the reaction mixture under vacuum, the residue is taken up in DCM and the solvent is evaporated off under vacuum. The residue is taken up in ether and the solvent is then decanted off. 1.5 g of the expected product are obtained in the form of a foam after drying, and this product is used without further purification in Example 2.

Preparation 2.3

2-Amino-3-[4-(N-propyl-N-methylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one dihydrochloride

[0358]

127

[0359] This compound is prepared according to Scheme 9.

A) 2-(tert-Butoxycarbonylamino)-3-(4-cyanophenyl)-1-(pyrrolidin-1-yl)propan-1-one

[0360] 7.5 g of BOP are added to a mixture of 4.06 g of 2-(tert-butoxycarbonylamino)-3-(4-cyanophenyl)propionic acid and 1.15 ml of pyrrolidine in 20 ml of DMF, and the mixture is stirred for 2 hours at RT, while maintaining the pH at 7 by adding N-ethylmorpholine. The reaction mixture is concentrated under vacuum and the residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (95/5; v/v). 3.8 g of the expected product are obtained.

B) 2-(tert-Butoxycarbonylamino)-3-(4-formylphenyl)-1-(pyrrolidin-1-yl)propan-1-one

[0361] A solution of 2 g of the compound obtained in the preceding step in 150 ml of a pyridine/AcOH/H2O mixture (2/1/1; v/v/v) is cooled to 0° C., 10.57 g of sodium hypophosphite hydrate are added under an argon atmosphere, followed by addition of 1.8 g of Raney® nickel in water, and the mixture is stirred for 10 minutes at RT. The reaction mixture is heated at 50° C. for 3 hours. After cooling to RT, the mixture is filtered through Celite®, washed with EtOH and then with DCM, and the filtrate is concentrated under vacuum. The residue is extracted with EtOAc, the organic phase is washed with water, with 5% KHSO4 solution, with saturated NaCl solution, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with EtOAc. 1.7 g of the expected product are obtained; m.p.=134° C.

C) 2-(tert-Butoxycarbonylamino)-3-[4-(N-propyl-N-methylamino methyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one

[0362] 0.42 ml of AcOH and then 0.707 g of sodium triacetoxyborohydride are added to a mixture of 0.77 g of the compound obtained in the preceding step and 0.34 ml of N-methylpropylamine in 10 ml of 1,2-dichloroethane, and the mixture is stirred overnight at RT. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.9 g of the expected product is obtained.

D) 2-Amino-3-[4-(N-propyl-N-methylaminomethyl)phenyl]-1-(pyrrolidin 1-yl)propan-1-one dihydrochloride

[0363] 5 ml of 10N HCl are added to a mixture of 0.88 g of the compound obtained in the preceding step in 10 ml of MeOH and 5 ml of dioxane, and the mixture is stirred for 4 hours at RT. EtOH is added and the reaction mixture is concentrated under vacuum. The residue is taken up in ether and the precipitate formed is filtered by suction after trituration. 0.83 g of the expected product is obtained; m.p.=140° C. (dec.).

Preparation 2.4

2-Amino-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]phenyl]-1-(pyrrolidin-1-yl)propan-1-one bis(trifluoroacetate)

[0364]

128

A) Ethyl 2-(benzhydrylideneamino)-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]phenyl]propionate

[0365] This compound is prepared according to the procedure described in step B of preparation 2.2, starting with 0.925 g of diethanolamine in 15 ml of DMF, 1.21 g of K2CO3 and 3.6 g of the compound obtained in step A) of preparation 2.2 in 20 ml of DMF. 4.23 g of the expected product are obtained in the form of an oil.

B) Ethyl 2-amino-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]phenyl]propionate

[0366] This compound is prepared according to the procedure described in step C) of preparation 2.2, starting with 4.22 g of the compound obtained in the preceding step and 70 ml of 1N HCl in 70 ml of ether. 1.87 g of the expected product is obtained in the form of a wax.

C) Ethyl 2-(tert-butoxycarbonylamino)-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]phenyl]propionate

[0367] This compound is prepared according to the procedure described in step D of preparation 2.2, starting with 1.87 g of the compound obtained in the preceding step in 20 ml of dioxane and 1.4 g of di-tert-butyl dicarbonate. 2.15 g of the expected product are obtained.

D) 2-(tert-Butoxycarbonylamino)-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]-phenyl]propionic acid

[0368] A mixture of 2.13 g of the compound obtained in the preceding step and 1.3 ml of 8.3N KOH in 15 ml of MeOH is stirred for 3 hours at RT. Next, a further 0.3 ml of 8.3N KOH are added and the mixture is stirred for one hour at RT. An EtOAc/H2O mixture is added to the reaction mixture and the resulting mixture is acidified to pH 3.7 by adding 1N HCl. After separation of the phases by settling, the organic phase is washed with water and with saturated NaCl solution, and dried over Na2SO4, and the solvent is evaporated off under vacuum to give a first crop of the expected product. The aqueous phase is concentrated under vacuum, the residue is taken up in an MeOH/2-propanol mixture (1/1; v/v), the insoluble material is filtered off and the filtrate is concentrated under vacuum to give a second crop. 1.66 g of the expected product are obtained in the form of a white solid.

E) 2-(tert-Butoxycarbonylamino)-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]-phenyl]-1-(pyrrolidin-1-yl)propan-1-one

[0369] This compound is prepared according to the procedure described in step F of preparation 2.2, starting with 0.95 g of the compound obtained in the preceding step in 12 ml of DMF, 0.6 ml of DIPEA, 0.24 ml of pyrrolidine and 1.44 g of BOP. The expected product is obtained in the form of a wax, which is used without further purification in the following step.

F) 2-Amino-3-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]phenyl] 1-(pyrrolidin-1-yl)propan-1-one bis(trifluoroacetate)

[0370] This compound is prepared according to the procedure described in step G) of preparation 2.2, starting with the compound obtained in the preceding step and 18 ml of TFA in 15 ml of DCM. The expected product is obtained, and is used without further purification in Example 4.

Preparation 2.5

1,2-Bis[4-(diethylaminomethyl)phenyl]ethylamine tris(trifluoroacetate)

[0371]

129

A) Methyl 4-(azidomethyl)benzoate

[0372] 8.13 g of sodium azide are added over 5 minutes to a solution of 5.73 g of methyl 4-(bromomethyl)benzoate in 30 ml of DMSO and the mixture is stirred for 3 hours 30 minutes at RT. The reaction mixture is extracted with ether, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 4.72 g of the expected product are obtained in the form of a colourless oil.

B) Methyl 4-(aminomethyl)benzoate hydrochloride

[0373] A solution of 4.71 g of the compound obtained in the preceding step in 30 ml of THF is cooled to 4° C., 6.57 g of triphenylphosphine are added portionwise over 30 minutes and the mixture is stirred for 6 hours while allowing the temperature to return to RT. Next, 0.68 ml of water is added and the mixture is stirred for 16 hours at RT. The reaction mixture is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvents are evaporated off under vacuum. The residue is taken up in ether, the insoluble material is filtered off, the filtrate is concentrated under vacuum and the residue is chromatographed on silica gel, eluting with a chloroform/MeOH/NH4OH mixture (90/10/0.2; v/v/v). The product obtained is taken up in MeOH, 10N HCl is added to pH 1 and the mixture is concentrated under vacuum. 4.25 g of the expected product are obtained.

C) Methyl 4-(benzhydrylideneaminomethyl)benzoate

[0374] A mixture of 3.03 g of the compound obtained in the preceding step, 2.07 ml of triethylamine and 2.51 ml of benzophenoneimine in 50 ml of DCM is stirred overnight at RT. The reaction mixture is extracted with DCM, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 5.33 g of the expected product are obtained in the form of an oil.

D) Methyl 4-[1-amino-2-(4-methoxycarbonylphenyl)ethyl]benzoate

[0375] A solution of 5.32 g of the compound obtained in the preceding step in 40 ml of THF is cooled to −50° C., 10.3 ml of a 1.6 M solution of n-butyllithium in hexane are added over 35 minutes and the mixture is stirred for 30 minutes at between −50° C. and −30° C. The reaction mixture is cooled again to −50° C., a solution of 3.78 g of methyl 4-(bromomethyl)benzoate in 25 ml of THF is added over 30 minutes and the mixture is stirred for 4 hours after the temperature has returned to RT. Next, 50 ml of 1N HCl are added and the reaction mixture is stirred for 16 hours at RT. The phases of the reaction mixture are allowed to separate by settling, the acidic phase is washed with ether, the acidic aqueous phase is basified to pH 10 by adding 10N NaOH and extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 3.88 g of the expected product are obtained in the form of a pasty white solid.

E) Methyl 4-[1-(tert-butoxycarbonylamino)-2-(4-methoxycarbonylphenyl)-ethyl]benzoate

[0376] 2.94 g of di-tert-butyl dicarbonate are added over 10 minutes to a solution of 3.88 g of the compound obtained in the preceding step in 30 ml of dioxane, and the mixture is stirred for 3 hours at RT. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with a KHSO4/K2SO4 buffer solution, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2.3 g of the expected product are obtained after crystallization from ether.

F) N-tert-Butoxycarbonyl-1,2-bis[4-(hydroxymethyl)phenyl]ethylamine

[0377] A solution of 1.24 g of the compound obtained in the preceding step in 15 ml of THF is added over 40 minutes to a suspension of 0.456 g of lithium aluminium hydride in 25 ml of THF, and the mixture is stirred for 2 hours at RT. An EtOAc/ice mixture is then added and the reaction mixture is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.95 g of the expected product is obtained in the form of a white solid.

G) 4-[2-(tert-Butoxycarbonylamino)-2-(4-methanesulphonyloxymethylphenyl)-ethyl]benzyl methanesulphonate

[0378] A solution of the compound obtained in the preceding step in 20 ml of DCM and 3 ml of THF is cooled to 4° C., 0.82 ml of triethylamine is added, followed by addition of a solution of 0.45 ml of methanesulphonyl chloride in 1 ml of DCM over 10 minutes, and the mixture is stirred for 3 hours 30 minutes at RT. The reaction mixture is extracted with DCM, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.21 g of the expected product are obtained.

H) N-(tert-Butoxycarbonyl)-1,2-bis [4-(diethylaminomethyl)phenyl]ethylamine

[0379] A mixture of 1.2 g of the compound obtained in the preceding step and 1.05 ml of diethylamine in 20 ml of EtOH is heated at 60° C. for 4 hours. After cooling to RT, the reaction mixture is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH/NH4OH mixture (85/15/0.2; v/v/v). 0.51 g of the expected product is obtained.

I) 1,2-bis[4-(Diethylaminomethyl)phenyl]ethylamine tris(trifluoroacetate)

[0380] A mixture of 0.5 g of the compound obtained in the preceding step and 12 ml of TFA in 10 ml of DCM is stirred for 45 minutes at RT. The reaction mixture is concentrated under vacuum and the residue is taken up in DCM and concentrated again under vacuum. 0.82 g of the expected product is obtained in the form of an oil.

Preparation 2.6

2-Amino-3-[4-(piperid-1-ylmethyl)phenyl]-1-(pyrrolidin-1-yl) propan-1-one dihydrochloride

[0381]

130

A) 2-(tert-Butoxycarbonylamino)-3-[4-(piperid-1-ylmethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one

[0382] This compound is prepared according to the procedure described in step C) of preparation 2.3, starting with 0.84 g of the compound obtained in step B) of preparation 2.3, 0.263 ml of piperidine, 10 ml of 1,2-dichloroethane, 0.452 ml of AcOH and 0.771 g of sodium triacetoxyborohydride. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The product obtained is taken up in ether, the insoluble material is filtered off and the filtrate is concentrated under vacuum. 0.975 g of the expected product is obtained in the form of an oil.

B) 2-Amino-3-[4-(piperid-1-ylmethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one dihydrochloride

[0383] This compound is prepared according to the procedure described in step D) of preparation 2.3, starting with 0.97 g of the compound obtained in the preceding step, 10 ml of MeOH, 5 ml of dioxane and 5 ml of 10N HCl. 0.91 g of the expected product is obtained; m.p.=170° C. (dec.)

[0384] NMR: 1.2-2.0 ppm: unres.: 10H; 2.4-3.4ppm: unres.: 1OH; 4.2ppm: bs: 3H; 7.2 ppm: d: 2H; 7.6 ppm: d: 2H; 8.4 ppm: s: 3H; 11.2 ppm: s: 1H.

Preparation 2.7

2-Amino-3-[4-(diethylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one dihydrochloride

[0385]

131

[0386] For its preparation steps D, E and F, this compound is obtained according to Scheme 10.

A) Ethyl 2-(benzhydrylideneamino)-3-[4-(diethylaminomethyl)phenyl]propionate

[0387] A mixture of 1 g of the compound obtained in step A) of preparation 2.2, 0.235 ml of diethylamine and 0.307 g of K2CO3 in 10 ml of DMF is stirred for 2 hours at RT. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.927 g of the expected product is obtained in the form of an oil.

B) Ethyl 2-amino-3-[4-(diethylaminomethyl)phenyl]propionate

[0388] A mixture of 0.927 g of the compound obtained in the preceding step and 20 ml of 1N HCl in 30 ml of ether is stirred for 2 hours at RT. After separation of the phases by settling, the acidic aqueous phase is washed with ether, the aqueous phase is basified to pH 11 by adding solid NaHCO3 and is extracted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.395 g of the expected product is obtained.

C) Ethyl 2-(tert-butoxycarbonylamino)-3-[4-(diethylaminomethyl)phenyl]propionate

[0389] 1.72 g of di-tert-butyl dicarbonate are added to a solution of 2 g of the compound obtained in the preceding step in 20 ml of DCM, followed by addition of 1.1 ml of triethylamine, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2.6 g of the expected product are obtained; m.p.=56° C.

D) 2-(tert-Butoxycarbonylamino)-3-[4-(diethylaminomethyl)phenyl]propionic acid

[0390] 10.1 ml of 1N KOH are added to a solution of 2.55 g of the compound obtained in the preceding step in 60 ml of EtOH and 20 ml of dioxane, and the mixture is heated for 1 hour at 60° C. After cooling to RT, 10 ml of 1N HCl are added and the reaction mixture is concentrated under vacuum. The residue is azeotroped by adding an EtOH/toluene mixture and is then concentrated under vacuum. The residue is taken up in a DCM/MeOH mixture (9/1; v/v), the insoluble material is filtered off and the filtrate is concentrated under vacuum. 2.4 g of the expected product are obtained.

E) 2-(tert-Butoxycarbonylamino)-3-[4-(diethylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one

[0391] 0.61 ml of triethylamine and then 2.12 g of BOP are added to a mixture of 1.53 g of the compound obtained in the preceding step and 0.365 ml of pyrrolidine in 10 ml of DMF, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into water and extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 1.5 g of the expected product are obtained.

F) 2-Amino-3-[4-(diethylaminomethyl)phenyl]-1-(pyrrolidin-1-yl)propan-1-one dihydrochloride

[0392] 6 ml of concentrated HCl are added to a solution of 1.5 g of the compound obtained in the preceding step in 20 ml of dioxane and 10 ml of MeOH, and the mixture is stirred overnight at RT. The reaction mixture is concentrated under vacuum and the residue is taken up in EtOH and concentrated again under vacuum. The residue is taken up in ether and the solvent is decantered off after trituration. 1.37 g of the expected product are obtained in the form of a gum.

[0393] Preparation 2.8

2-Amino-3-[4-(diethylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0394]

132

A) 2-(tert-Butoxycarbonylamino)-3-[4-(diethylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide

[0395] 0.279 ml of triethylamine is added to a mixture of 0.7 g of the compound obtained in step D) of preparation 2.7 and 0.208 ml of N-methylisopropylamine in 10 ml of DMF, followed by addition of 0.973 g of BOP, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into water and extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.65 g of the expected product is obtained.

B) 2-amino-3-[4-(diethylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0396] 5 ml of TFA are added to a solution of 0.63 g of the compound obtained in the preceding step in 5 ml of DCM, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in iso ether and the solvent is decantered off after trituration. The expected product is obtained after drying under vacuum.

Preparation 2.9

2-amino-3-[4-(diethylaminomethyl)phenyl]-N,N-diisopropylpropionamide dihydrochloride

2HCl: Y═—CO—N(iPr)2; Z=-CH2—N(Et)2 (III),

A) 2-(tert-Butoxycarbonylamino)-3-(4-cyanophenyl)-N,N-diisopropylpropionamide

[0397] A mixture of 1 g of 2-(tert-butoxycarbonylamino)-3-(4-cyanophenyl)propionic acid and 0.540 g of diisopropylamine in 10 ml of DCM is cooled to 0° C., 0.960 ml of triethylamine is added, followed by addition of 1.76 g of bromotripyrrolidinophosphonium hexafluorophosphate, and the mixture is stirred for 2 hours and allowed to warm to RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water, with 5% KHSO4 solution, with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a heptane/EtOAc mixture (80/20; v/v). 0.4 g of the expected product is obtained; m.p.=172° C.

B) 2-(tert-Butoxycarbonylamino)-3-(4-formylphenyl)-N,N-diisopropylpropionamide

[0398] This compound is prepared according to the procedure described in step B of preparation 2.3, starting with 0.380 g of the compound obtained in the preceding step, 25 ml of a pyridine/AcOH/H2O mixture (2/1/1; v/v/v), 1,8 g of sodium hypophosphite hydrate and 0.31 g of Raney® nickel. 0.35 g of the expected product is obtained without performing chromatography; m.p.=152° C.

C) 2-(tert-Butoxycarbonylamino)-3-[4-(diethylaminomethyl)phenyl)-N,N-diisopropylpropionamide

[0399] This compound is prepared according to the procedure described in step C of preparation 2.3, starting with 0.333 g of the compound obtained in the preceding step, 0.135 ml of diethylamine, 10 ml of 1,2-dichloroethane, 0.08 ml of AcOH and 0.281 g of sodium triacetoxyborohydride. 0.4 g of the expected product is obtained.

D) 2-Amino-3-[4-(diethylaminomethyl)phenyl]-N,N-diisopropylpropionamide dihydrochloride

[0400] 5 ml of concentrated HCl are added to a mixture of 0.4 g of the compound obtained in the preceding step in 10 ml of MeOH and 5 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is concentrated under vacuum and the residue is taken up in EtOH and evaporated under vacuum. 0.37 g of the expected product is obtained.

Preparation 2.10

2-[4-(Diethylaminomethyl)phenyl]-1-(pyrid-3-yl)ethylamine tris(trifluoroacetate)

[0401]

133

A) Benzhydrylidenepyrid-3-ylmethylamine

[0402] 1.7 ml of 3-(aminomethyl)pyridine are added to a solution of 2.8 ml of benzophenoneimine in 50 ml of DCM, and the mixture is stirred overnight at RT. A further 0.28 ml of benzophenoneimine is added and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with ether, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 4.56 g of the expected product are obtained.

B) Methyl 4-[2-amino-2-(pyrid-3-yl)ethyl]benzoate

[0403] A solution of 3 g of the compound obtained in the preceding step in 30 ml of THF is cooled to −78° C., under an argon atmosphere, 8.2 ml of a 1.5 M solution of lithium diisopropylamide in cyclohexane are added and the mixture is stirred for 30 minutes while allowing the temperature to rise to −20° C. The mixture is cooled again to −78° C., a solution of 2.82 g of methyl 4-(bromomethyl)benzoate in 10 ml of THF is added dropwise and the mixture is stirred for 2 hours while allowing the temperature to rise to RT. The reaction mixture is cooled to 0° C., 25 ml of 1N HCl are added slowly, followed by addition of concentrated HCl solution to pH 2-3, and the mixture is stirred overnight at RT. An ether/water mixture (1/1; v/v) is added to the reaction mixture and, after separation of the phases by settling, the organic phase is extracted with 1N HCl solution, the combined aqueous phases are basified to pH 8 by adding concentrated NaOH and extracted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2 g of the expected product are obtained.

C) Methyl 4-[2-(tert-butoxycarbonylamino)-2-(pyrid-3-yl)ethyl]benzoate

[0404] 1.83 g of di-tert-butyl dicarbonate are added to a solution of 1.95 g of the compound obtained in the preceding step in 20 ml of DCM, followed by addition of 1.16 ml of triethylamine, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a heptane/EtOAc mixture of from (60/40; v/v) to (40/60; v/v). 1.3 g of the expected product are obtained.

D) N-tert-Butoxycarbonyl-2-(4-hydroxymethylphenyl)-1-(pyrid-3-yl)ethylamine

[0405] A solution of 1.13 g of the compound obtained in the preceding step in 30 ml of THF is cooled to −78° C., under an argon atmosphere, 7.2 ml of a 1M solution of diisobutylaluminium hydride in toluene are added dropwise and the mixture is stirred for 2 hours while allowing the temperature to rise to 0° C. The reaction mixture is cooled to −40° C., a further 7.2 ml of a 1M solution of diisobutylaluminium hydride in toluene are added and the mixture is stirred for 2 hours while allowing the temperature to rise to 0° C. 40 ml of saturated NH4Cl solution are added, the mixture is extracted with EtOAc, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/EtOAc mixture (40/60; v/v). 0.62 g of the expected product is obtained after crystallization from ether; m.p.=130° C.

E) N-(tert-butoxycarbonyl)-2-[4-(diethylaminomethyl)phenyl]-1-(pyrid-3-yl)ethylamine

[0406] A solution of 0.61 g of the compound obtained in the preceding step in 20 ml of DCM is cooled to 0° C., 0.311 ml of triethylamine is added, followed by addition of 0.16 ml of methanesulphonyl chloride, and the mixture is stirred for 30 minutes at 0° C. Next, 0.58 ml of diethylamine is added and the mixture is stirred for 3 hours at RT. The reaction mixture is poured into water and extracted with DCM, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.45 g of the expected product is obtained.

F) 2-[4-(Diethylaminomethyl)phenyl]-l-(pyrid-3-yl)ethylamine tris(trifluoro-acetate)

[0407] A mixture of 0.45 g of the compound obtained in the preceding step and 10 ml of TFA in 10 ml of DCM is stirred for 15 minutes at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in ether, the solvent is decantered off and the expected crude product is used without further purification.

Preparation 2.11

Ethyl 2-amino-3-[4-(N-ethyl-N-methylaminomethyl)phenyl]propionate

[0408]

134

A) Ethyl 2-(benzhydrylideneamino)-3-[4-(N-ethyl-N-25 methylaminomethyl)phenyl]propionate

[0409] A mixture of 1 g of the compound obtained in step A of Preparation 2.2 and 0.29 ml of N-methylethylamine in 10 ml of DMF is cooled to 0° C., 0.307 g of K2CO3 is added and the mixture is stirred for 4 hours at 0° C. The reaction mixture is poured into saturated NaCl solution and extracted with EtOAc, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The expected product is obtained and is used without further purification.

B) Ethyl 2-amino-3-[4-(N-ethyl-N-methylaminomethyl)phenyl]propionate

[0410] A mixture of the compound obtained in the preceding step and 15 ml of 1N HCl in 15 ml of ether is stirred overnight at RT. After separation of the phases by settling, the aqueous phase is basified to pH 11 by adding Na2CO3 and is extracted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.43 g of the expected product is obtained, and is used without further purification in EXAMPLE 13.

Preparation 2.12

Ethyl 2-amino-3-[4-(dipropylaminomethyl)phenyl]propionate

[0411] (III): Y=—CO2Et; Z=-CH2N(nPr)2

A) Ethyl 2-(benzhydrylideneamino)-3-[4-(dipropylaminomethyl)phenyl]propionate

[0412] This compound is prepared according to the procedure described in step A of Preparation 2.11, starting with 1 g of the compound obtained in step A of Preparation 2.2, 0.335 ml of dipropylamine and 0.307 g of K2CO3 in 10 ml of DMF. The expected product is obtained, and is used without further purification.

B) Ethyl 2-amino-3-[4-(dipropylaminomethyl)phenyl]propionate

[0413] This compound is prepared according to the procedure described in step B of Preparation 2.11, starting with the compound obtained in the preceding step and 15 ml of 1N HCl in 15 ml of ether. The expected product is obtained and is used without further purification.

Preparation 2.13

Ethyl 2-amino-3-[4-(diisopropylaminomethyl)phenyl]propionate

Y═—CO2Et; Z=-CH2N(iPr)2 (III):

A) Ethyl 2-(benzhydrylideneamino)-3-[4-(diisopropylaminemethyl)phenyl]propionate

[0414] This compound is prepared according to the procedure described in step A of Preparation 2.11, starting with 1 g of the compound obtained in step A of Preparation 2.2, 0.320 ml of diisopropylamine and 0.307 g of K2CO3 in 10 ml of DMF. The expected product is obtained and is used without further purification.

B) Ethyl 2-amino-3-[4-(diisopropylaminomethyl)phenyl]propionate

[0415] This compound is prepared according to the procedure described in step B of Preparation 2.11, starting with the compound obtained in the preceding step and 30 ml of 1N HCl in 30 ml of ether. 0.5 g of the expected product is obtained.

Preparation 2.14

2-Amino-3-[4-(tert-butylmethylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate), (R) isomer

[0416]

135

[0417] This compound is prepared according to the procedure described in Preparation 2.3, using 2-(tert-butoxycarbonylamino)-3-[4-(tert-butylmethylaminomethyl)phenyl]propionic acid, (R) isomer, as starting material.

[0418] NMR: 0.8-1.2 ppm: mt: 6H; 1.4 ppm: s: 9H; 2.5 ppm: d: 6H; 3.0 ppm: unres.: 2H; 3.9 ppm: mt: 1H; 4.5 ppm: mt: 2H; 7.3-7.5 ppm: mt: 4H.

Preparation 2.15

2-Amino-3-[4-(piperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide dihydrochloride

[0419]

136

A) 2-(tert-Butoxycarbonylamino)-3-(4-cyanophenyl)-N-isopropyl-N-methylpropionamide

[0420] This compound is prepared according to the procedure described in Preparation 1.6 of International patent application WO 97/25315.

B) 2-(tert-Butoxycarbonylamino)-3-(4-formylphenyl)-N-isopropyl-N-methylpropionamide

[0421] A solution of 9 g of the compound obtained in the preceding step in 600 ml of a pyridine/AcOH/H2O mixture (2/1/1; v/v/v) is cooled to 0° C., 47 g of sodium hypophosphite hydrate are added under an argon atmosphere, follwed by addition of 8 g of Raney® nickel in water, and the mixture is stirred for 10 minutes at RT. The reaction mixture is heated at 55° C. for 3 hours. After cooling to RT, the mixture is filtered through Celite®, washed with EtOH and then with DCM, and the filtrate is concentrated under vacuum. The residue is extracted with EtOAc, the organic phase is washed with water, with 5% KHSO4 solution, with saturated NaCl solution, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 7.6 g of the expected product are obtained after crystallization from an iso ether/pentane mixture; m.p.=122° C.

C) 2-(tert-Butoxycarbonylamino)-3-[4-(piperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide

[0422] 0.18 ml of AcOH is added to a mixture of 0.55 g of the compound obtained in the preceding step and 0.172 ml of piperidine in 10 ml of 1,2-dichloroethane, followed by addition of 0.5 g of sodium triacetoxyborohydride, and the mixture is stirred overnight at RT. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.65 g of the expected product is obtained.

D) 2-Amino-3-[4-(piperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide dihydrochloride

[0423] 3.5 ml of 10N HCl are added to a mixture of 0.65 g of the compound obtained in the preceding step in 10 ml of MeOH and 5 ml of dioxane, and the mixture is stirred overnight at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in EtOH and the solvent is evaporated off under vacuum. The residue is taken up in ether and the precipitate formed is filtered off by suction, after trituration. 0.64 g of the expected product is obtained; m.p.=165° C. (dec).

Preparation 2.16

2-Amino-3-[4-(N-cyclopentyl-N-methylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0424]

137

A) 2-(tert-Butoxycarbonylamino)-3-[4-(N-cyclopentyl-N-methylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide

[0425] 0.164 ml of AcOH is added to a mixture of 0.5 g of the compound obtained in step B of Preparation 2.15 and 0.214 g of N-methylcyclopentylamine in 10 ml of 1,2-dichloroethane, followed by addition of 0.456 g of sodium triacetoxyborohydride, and the mixture is stirred overnight at RT. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaHCO3 solution and the solvent is evaporated off under vacuum. The residue is taken up in EtOAc and extracted with pH 4 buffer, the acidic aqueous phase is washed with EtOAc, basified by addition of saturated NaHCO3 solution and extracted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.31 g of the expected product is obtained.

B) 2-Amino-3-[4-(N-cyclopentyl-N-methylaminomethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0426] A mixture of 0.309 g of the compound obtained in the preceding step and 10 ml of TFA in 10 ml of DCM is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum. The product obtained is used without further purification in Example 36.

Preparation 2.17

2-Amino-3-[4-(4-hydroxypiperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0427]

138

A) 2-(tert-Butoxycarbonylamino)-3-[4-(4-hydroxypiperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide

[0428] 0.164 ml of AcOH is added to a mixture of 0.5 g of the compound obtained in step B of Preparation 2.15 and 0.146 g of 4-hydroxypiperidine in 10 ml of 1,2-dichloroethane, followed by addition of 0.456 g of sodium triacetoxyborohydride, and the mixture is stirred for 24 hours at RT. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.622 g of the expected product is obtained.

B) 2-Amino-3-[4-(4-hydroxypiperid-1-ylmethyl)phenyl]-N-isopropyl-N-methylpropionamide bis(trifluoroacetate)

[0429] A mixture of 0.622 g of the compound obtained in the preceding step and 10 ml of TFA in 10 ml of DCM is stirred for 1 hour at RT. The reaction mixture is concentrated under vacuum. The expected product is obtained, and is used without further purification.

Preparation 2.18

(R) 1-[2-Amino-3-(4-cyanophenyl)propionyl)pyrrolidine trifluoroacetate

[0430]

139

A) Ethyl 2-(N-Boc)amino-3-(4-cyanophenyl)propionate

[0431] 26 g of Boc2O dissolved in 100 ml of DCM are added gradually to a solution of 25.5 g of ethyl 2-amino-3-(4-cyanophenyl)propionate hydrochloride and 13.9 ml of Et3N in 400 ml of DCM. After stirring for 6 hours at RT, the reaction medium is washed with a KHSO4/K2SO4 solution, with saturated NaHCO3 solution and with saturated NaCl solution. After drying over Na2SO4 and evaporation of the DCM, the residue is triturated in heptane to give 29 g of a white powder.

B) Ethyl (R) 2-(N-Boc)amino-3-(4-cyanophenyl)propionate

[0432] A mixture of 24 g of the product obtained in the preceding step and 8.4 g of NaHCO3 in 900 ml of EtOAc and 500 ml of H2O is treated with 2 ml of Alcalase® for 24 hours at RT. The 2 phases are separated by settling; the EtOAc phase is washed again with 100 ml of a 10% NaHCO3 solution, which is combined to the first aqueous phase, and the aqueous phase is washed again with 100 ml of EtOAc, which are combined with the first EtOAc phase. The EtOAc phase thus obtained is dried over Na2SO4 and then evaporated to dryness; 12.45 g of compound B are obtained.

αD25=+8.8°(c=1; MeOH)

C) (R) 2-(N-Boc)amino-3-(4-cyanophenyl)propionic acid

[0433] 43 ml of a 1N NaOH solution are added to 12.18 g of compound B dissolved in 180 ml of MeOH, and the mixture is stirred for 1 hour at RT. Next, 43 ml of 1N HCl solution are added and 150 ml of methanol are evaporated off, the residue is then taken up in EtOAc and washed with water and then with saturated NaCl solution. 11 g of the expected compound are obtained, after crystallization from an Et2O/heptane mixture.

αD25=+−9.5°(c=1; MeOH)

D) N-hydroxysuccinimide (R) 2-(N-Boc)amino-3-(4-cyanophenyl)propionate

[0434] 4.2 g of NSuOH are added to 10 g of the acid obtained above dissolved in 10 ml of dioxane, followed by addition over 20 minutes of 8.62 g of DCC dissolved in 30 ml of dioxane. After stirring overnight at RT, the DCU formed is filtered off and washed with dioxane. The filtrate is evaporated to dryness and the residue is triturated in ether to give a solid, which is filtered off and dried. 12.09 g of the expected compound are obtained.

αD25+27.1°(c=1; MeOH)

E) (R) 1-[2-(N-Boc)amino-3-(4-cyanophenyl)propionyl]pyrrolidine

[0435] 2.6 ml of pyrrolidine dissolved in 20 ml of acetonitrile are added over 10 minutes to 11.6 g of the compound obtained in the above step dissolved in 150 ml of acetonitrile plus 20 ml of DMF. After stirring overnight at RT, a small amount of insoluble material is removed and the filtrate is concentrated under vacuum. The residue is taken up in EtOAc and washed with KHSO4/K2SO4 solution, with saturated NaHCO3 solution and with saturated NaCl solution; after drying over Na2SO4, the EtOAc is evaporated off under vacuum and the residue is triturated in ether to give 9.3 g of the expected compound in the form of a white solid.

αD25=−29.2°(c=1; MeOH)

F) (R) 1-[2-Amino-3-(4-cyanophenyl)propionyl]pyrrolidine trifluoroacetate

[0436] 8.7 g of the product obtained in the preceding step are stirred for 35 minutes in a mixture of 50 ml of DCM and 50 ml of TFA. After evaporation to dryness, the residue is taken up in isopropanol and re-evaporated to dryness, to give 8.67 g of the expected compound in solid form.

αD25=−46°(c=1; MeOH)

Preparation 2.19

(R) 2-amino-3-(4-diisopropylaminomethylphenyl)-N-isopropyl-N-methylpropionamide trifluoroacetate

[0437]

140

A) (R) 2-(N-Boc)amino-3-(4-cyanophenyl)-N-isopropyl-N-methylpropionamide

[0438] 2 g of the acid obtained in Preparation 2.18, step C are placed in 20 ml of DCM in the presence of 750 μl of isopropylmethylamine, 1.26 ml of DIPEA and 3.2 g of BOP, and the mixture is then stirred for 4 hours at RT. The reaction mixture is concentrated to dryness and is then extracted with EtOAc, followed by washing successively with water, with pH 2 buffer solution, with saturated NaHCO3 solution and with saturated NaCl solution. 2.40 g of the expected compound are obtained.

B) (R) 2-(N-Boc)amino-3-(4-formylphenyl)-N-isopropyl-N-methylpropionamide

[0439] 2.4 g of the compound from the preceding step are placed in 130 ml of solvent consisting of a pyridine/AcOH/water mixture (2/1/1; v/v/v); 2.45 g of Raney nickel and 12.12 g of NaH2PO2 are added and the mixture is heated at 55° C. for 3 hours. The reaction mixture is filtered through Celite and the filtrate is concentrated. The residue is extracted with EtOAc and then washed successively with water, with pH 2 buffer solution, with saturated NaHCO3 solution and with saturated NaCl solution. After concentration and drying, the residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (100/1; v/v). 1.60 g of the expected compound are obtained.

C) (R) 2-(N-Boc)amino-3-(4-diisopropylaminomethyl)-N-isopropyl-N-methylpropionamide

[0440] A mixture containing 0.5 g of the compound obtained in the preceding step, 402 μl of diisopropylamine and 608 mg of NaBH(OAc)3 in 20 ml of DCE is stirred for 24 hours at RT. The reaction mixture is concentrated to dryness, the residue is taken up in a water/EtOAc mixture and is then extracted with DCM and washed with saturated NaCl solution. 0.296 g of the expected compound is obtained.

D) (R) 2-Amino-3-(4-diisopropylaminomethylphenyl)-N-isopropyl-N-methylpropionamide trifluoroacetate

[0441] 296 mg of the compound from the preceding step are placed in 5 ml of TFA and 10 ml of DCM, and the mixture is stirred for 3 hours at RT. The reaction mixture is concentrated to dryness, the residue is triturated in pentane, the solvent is decantered off and the oil formed is then recovered. The compound obtained is used in crude form in Example 39.

[0442] By working according to the methods described above, the compounds of formula (III) described in Table V below are prepared:

5TABLE V(III)′ = (III) or (XXXVII)

141

NMRPreparationTY′Zor m.p. ° C.2.20HCO2H

142

NMR2.21 2HClH

143

144

NMR2.22Boc

145

146

68-70° C. NMR2.23 2HClH

147

148

170° C.2.24Boc

149

150

NMR2.25Boc

151

152

102° C. NMR2.26Boc

153

154

85° C.2.27Boc

155

156

75° C.2.28 2HClH

157

—CN267° C.2.29H

158

—CN62° C. NMR2.30 2HClH

159

160

2.31Boc

161

162

NMR2.32Boc

163

164

128-130° C. NMR2.33Boc

165

166

NMR2.34Boc

167

168

NMR2.35 2HClH

169

170

166° C.2.36Boc

171

172

66-68° C. NMR2.37Boc

173

174

99-102° C. NMR2.38Boc

175

176

NMR2.39H

177

—CN92° C.2.40H

178

—CN

Preparation 2.20

[0443] NMR (DMSO+TFA): 2.8 ppm: s: 3H; 3.1 ppm: mt: 2H; 3.5 ppm: unres.: 8H; 4.2 ppm: t: 1H; 4.4 ppm: s: 2H; 7.35 ppm: d: 2H; 7.6 ppm: d: 2H.

Preparation 2.21

[0444] NMR: 1.25 ppm: s: 9H; 1.40-1.65 ppm: unres.: 4H; 2.40-4.60 ppm: mt: 10H; 7.10-7.60 ppm: mt: 4H.

Preparation 2.22

[0445] NMR: 0.85-1.00 ppm: t: 6H; 1.20 ppm: s: 9H; 2.30-4.30 ppm: unres.: 8H; 4.70 ppm: q: 1H; 7.10-7.75 ppm: unres.: 10H.

Preparation 2.24

[0446] NMR (DMSO+TFA): 1.0 ppm: mt: 6H; 1.2 ppm: s: 9H; 1.8 ppm: unres.: 14H; 2.6 ppm: d: 3H; 3.0 ppm: unres.: 6H; 4.1-4.7 ppm: unres.: 4H; 7.3 ppm: q: 4H.

Preparation 2.25

[0447] NMR: 1.0 ppm: s: 9H; 1.2 ppm: s: 9H; 1.9 ppm: s: 3H; 2.8 ppm: d: 2H; 3.4 ppm: s: 2H; 4.6 ppm: mt: 1H; 7.0-7.3 ppm: mt: 9H; 7.4 ppm: d: 1H.

Preparation 2.29

[0448] NMR: 2.9 ppm: mt: 2H; 4.0 ppm: t: 1H; 6.1 ppm: d: 1H; 6.3 ppm: d: 1H; 7.3 ppm: d: 2H; 7.5 ppm: s: 1H; 7.6 ppm: d: 2H.

Preparation 2.31

[0449] NMR: 0.85 ppm: t: 6H; 1.20 ppm: s: 9H; 2.40-4.30 ppm: unres.: 1H; 6.90-8.05 ppm: unres.: 9H.

Preparation 2.32

[0450] NMR: 0.90 ppm: t: 6H; 1.20 ppm: s: 9H; 2.40 ppm: q: 4H; 3.20-3.70 ppm: mt: 7H; 5.10 ppm: q: 11H; 7.10-7.70 ppm: mt: 9H.

Preparation 2.33

[0451] NMR (DMSO+TFA): 1.15 ppm: t: 6H; 1.20 ppm: s: 9H; 1.60-1.80 ppm: mt: 2H; 2.70-4.40 ppm: unres.: 19H; 7.25-7.45 ppm: mt: 4H.

Preparation 2.34

[0452] NMR (DMSO+TFA): 1.10 ppm: t: 6H; 1.20 ppm: s: 9H; 2.60-4.60 ppm: unres.: 20H; 7.20-7.45 ppm: mt: 4H.

Preparation 2.36

[0453] NMR: 1.00 ppm: s: 9H; 1.15 ppm: s: 9H; 2.70-4.60 ppm: unres.: 8H; 7.00-8.40 ppm: unres.: 9H.

Preparation 2.37

[0454] NMR: 0.90 ppm: t: 6H; 1.20 ppm: s: 9H; 2.35 ppm: q: 4H; 2.80-3.40 ppm: unres.: 4H; 4.80-5.00 ppm: mt: 1H; 7.00-7.50 ppm: unres.: 9H; 12.1 ppm: bs: 1H.

Preparation 2.38

[0455] NMR: 1.2 ppm: s: 9H; 1.2-1.8 ppm: unres.: 8H; 1.9ppm: s: 3H; 2.6 ppm: mt: 11H; 2.8 ppm: d: 2H; 3.3 ppm: s: 2H; 4.6 ppm: mt: 11H; 7.0-7.2 ppm: mt: 9H; 7.4 ppm: d: 11H.

Preparation 2.41

(R) 2-Amino-3-(4-((2,6-dimethyl-1-piperidyl)methyl)phenyl-N-isopropyl-N-methylpropanamide, 2TFA

[0456]

179

A)

[0457] A mixture containing 500 mg of the compound described in Preparation 2.19, step B, 390 μl of (cis)-2,6-dimethylpiperidine and 608 mg of NaBH(OAc)3 is stirred for 48 hours at RT. The mixture is concentrated to dryness and then diluted with 30 ml of EtOAc and extracted with a pH 4 buffer solution. The aqueous phase is extracted with DCM and then washed with saturated NaHCO3 solution and with saturated NaCl solution. 411 mg of the expected compound are obtained.

B)

[0458] 411 mg of the compound obtained in the preceding step and 10 ml of TFA in 10 ml of DCM are stirred for 4 hours at RT. The mixture is concentrated to dryness and then triturated in a pentane/ether mixture. After decantation and drying, 0.480 g of the expected compound is obtained in the form of an oil.

Preparation 2.42

(R) 2-N-Boc)amino-3-(4-((3,3-dimethyl-1-piperidyl)methylphenyl)-N-isopropyl)-N-methylpropanamide

[0459]

180

[0460] 497 mg of the compound described in Preparation 2.19, step B are placed in 10 ml of DCE and 165 μl of AcOH and 455 mg of NaBH(OAc)3 are added. After stirring overnight at RT, the reaction medium is concentrated and then taken up in Et2O/H2O and extracted with a pH 4 buffer. The aqueous phases are basified to pH 8 with caustic soda and then extracted with DCM and washed with saturated NaCl solution. 0.168 g of the expected compound is obtained.

Preparation 2.43

(R) 2-(N,Boc)amino-3-(4-((4,4-difluoro-1-piperidyl)methyl)phenyl-N-isopropyl-N-methylpropanamide

[0461]

181

[0462] This compound is prepared as described in Preparation 2.41, starting with the compound of Preparation 2.19, step B and 4,4-difluoropiperidine.

Preparation 2.44

2-(R)-2-(N-Boc)amino-3-(4-((2,6-dimethyl-1-piperidyl)methyl)phenyl)propanoic acid

[0463]

182

[0464] This compound is prepared by following Schemes 9 and 10.

A) Ethyl 2-(R)-2-(N-Boc)amino-3-(4-formylphenyl)propanoate

[0465] 4 g of the compound from Preparation 2.18, step B are placed in 300 ml of a pyridine/AcOH/H2O mixture (2/1/1; v/v/v) under argon. This mixture is treated with 5 g of Raney nickel and 30 g of NaH2PO2H2O for 6 hours at 55° C. The reaction mixture is filtered through Celite® and then concentrated. The residue is taken up in EtOAc, washed with H2O, with 5% KHSO4, with H2O, with saturated NaHCO3 solution and with saturated NaCl solution and then dried and concentrated. 3.91 g of the expected product are obtained in the form of an oil.

[0466] NMR: 1.2: t: 3H; 1.4: s: 9H; 2.9-3.2: mt: 2H; 4.0-4.4: mt: 3H; 7.4: d: 1H; 7.55: d: 2H; 7.9: d: 2H; 10.0: s: 1H.

B) Ethyl 2-(R)-2-(N-Boc)amino-3-(4-((2,6-dimethyl-1-piperidyl)methyl)phenyl-propanoate

[0467] 3.9 g of the compound obtained in step B are placed in 30 ml of dichloroethane and the solution is treated with 6.7 g of NaHB(OAc)3 and 4 ml of (cis)-2,6-dimethylpiperidine for 24 hours at RT. The reaction mixture is concentrated and the residue is taken up in ether and then extracted with 5% KHSO4 and then with water. The aqueous phases are basified to pH 8 by adding NaHCO3 and extracted with DCM, and the organic phase is dried and concentrated to give 3 g of the expected product in the form of an oil.

[0468] NMR: 0.9: d: 6H; 1.0: t: 3H; 1.05-1.8: mt: 15H; 2.3: unres.: 2H; 2.6-2.9: mt: 3.6: s: 2H; 2.9-4.1: mt: 3H; 7.0-7.2: mt: 5H.

C) (R)-2-(N-Boc)amino-3-(4-((2,6-dimethyl-1-piperidyl)methyl)phenyl)propanoic acid

[0469] 3 g of the compound obtained in the preceding step are placed in 20 ml of MeOH and 20 ml of dioxane, followed by dropwise addition of 7.5 ml of 1N NaOH, and the mixture is stirred for 1 hour at RT. 7.5 ml of 1N HCl are added and the mixture is then concentrated to dryness. An azeotropic distillation is carried with absolute EtOH and toluene. The mixture is taken up in DCM, dried and filtered to give 2.7 g of the expected compound in the form of a dry foam.

[0470] NMR: 0.9: d: 6H; 1.0-1.4: mt: 15H; 2.4: unres.: 2H; 2.6-3.0: mt: 2H; 3.65: s: 4.0: unres.: 1H; 7.0: d: 1H; 7.2: d: 2H.

Preparation 2.45

2-(R)-2-(N-Boc)amino-3-(4-(((2-fluoroethyl)(ethyl)amino)methyl)phenyl)-N-isopropyl-N-methylpropanamide

[0471]

183

[0472] This compound is prepared according to Scheme 9a (step b) of the above description.

A) 2-(R)-2-(N-Boc)amino-3-(4-(((ethylamino)methyl)phenyl)-N-isopropyl-N-methyl)propanamide

[0473] 520 mg of 2-(R)-2-(N-Boc)-3-(4-formylphenyl)-N-isopropyl-N-methylpropanamide are placed in 20 ml of DCE and treated with 3 ml of ethaneamine and 633 mg of NaHB(OAc)3 plus 1 ml of AcOH, overnight at RT. The reaction mixture is concentrated and then taken up in a water/ether mixture. The aqueous phase is basified to pH 8 with NaHCO3 and then extracted with DCM. 350 mg of the expected compound are obtained in the form of an oil. The NMR spectrum is in accordance with the structure of the compound.

B)

[0474] 310 mg of the compound from the preceding step are dissolved in DMF (10 ml) and treated with 2-fluoro-1-iodoethane (143 mg) in the presence of K2CO3 (114 mg) for 48 hours at RT. The reaction is concentrated and the residue is taken up in EtOAc and extracted with H2O and then with 5% KHSO4. The aqueous phase is basified to pH 8 with NaHCO3 and then extracted with CH2Cl2. 115 mg of the expected product are obtained in the form of an oil.

[0475] NMR

Preparation 2.46

2-(R)-2-(N-Boc)amino-3-(4-(((cyclopropyl)(isopropyl)amino)methyl)phenyl)-N-isopropyl-N-methyl)propanamide

[0476]

184

[0477] This compound is prepared according to Scheme 9a (step c).

A) 2-(R)-2-(N-Boc)amino-3-(4-((cyclopropylamino)methyl)phenyl)-N-isopropyl-N-methyl)propanamide

[0478] This compound is obtained by reacting cyclopropylamine with 2-(R)-2-(N-Boc)-3-(4-formylphenyl)-N-isopropyl-N-methylpropanamide according to the procedure described in step A of the above preparation.

B) 2-(R)-2-(N-Boc)amino-3-(4-(((cyclopropyl)(isopropyl)amino)methyl)phenyl)-N-isopropyl-N-methyl)propanamide

[0479] 220 mg of the compound from the preceding step and 125 μl of acetone are placed in 10 ml of DCE and treated with 180 mg of NaHB(OAc)3 for 24 hours at RT. The reaction mixture is concentrated and is then taken up in ether and extracted with 5% KHSO4 solution and then with H2O. The aqueous phases are basified to pH 8 with NaHCO3 and then extracted with DCM and dried. 105 mg of the expected compound are obtained in the form of an oil.

[0480] By working according to the preparations described above, the intermediate compounds described in the table below are prepared. (The reaction process used for the preparation is indicated in the right-hand column.)

6TABLE VI

185

Preparations(stereochemistry)CONR8R9—CH2NR11R12m.p. ° C. or NMRProcess2.47 (R)

186

187

NMRa2.48

188

189

NMRa2.49

190

191

NMRa2.50

192

193

NMRa2.51

194

195

NMRa2.52 (R)

196

197

NMRa2.53

198

199

NMRc2.54 (R)

200

201

crude product usedc2.55 (R)

202

203

NMRa2.56

204

205

crude product useda2.57

206

207

NMRa2.58

208

209

NMRd2.59

210

211

NMRa2.60

212

213

NMRa2.61

214

215

NMRa2.62

216

217

NMRa2.63

218

219

NMRa2.64

220

221

NMRa2.65 (R)

222

223

NMRa2.66 (R)

224

225

NMRd2.67

226

227

NMRa2.68

228

229

NMRc2.69 (R)

230

231

NMRa2.70 (R)

232

233

NMRa2.71

234

235

NMRa2.72 (R)

236

237

NMRa2.73

238

239

NMRd2.74 (R)

240

241

NMRd2.75 (R)

242

243

NMRd2.76

244

245

NMRa2.77 (R)

246

247

NMRa2.78

248

249

NMRa2.79

250

251

NMRa2.80

252

253

NMRa2.81 (R)

254

255

NMRa2.82

256

257

103° C.a2.83 (R)

258

259

NMRa2.84

260

261

NMRa2.85

262

263

NMRa2.86 (R)

264

265

NMRa2.87

266

267

crude productd2.88

268

269

130° C.d2.89 (R)

270

271

NMRa2.90 (R)

272

273

NMRa2.91 (R)

274

275

crude product useda2.92 (R)

276

277

NMRd2.93 (R)

278

279

NMRd2.94 (R)

280

281

NMRd2.95 (R)

282

283

NMRd2.96 (R)

284

285

NMRd2.97 (R)

286

287

NMRa2.98 (R)

288

289

NMRd2.99 (R)

290

291

NMRd2.100 (R)

292

293

152° C.d2.101 (R)

294

295

NMRd2.102 (R)

296

297

NMRa2.103 (R)

298

299

NMRd2.104 (R)

300

301

120° C.a2.105 (R)

302

303

NMRa2.106

304

305

NMRa2.107 (R)

306

307

NMRa2.108

308

309

NMRa2.109 (R)

310

311

NMRa2.110 (R)

312

313

NMRa2.111 (R)

314

315

NMRa2.112 (R)

316

317

NMRaa This compound is prepared from a compound (III) in which Z = CN and Y = CONR8R9, using the process described in Scheme 9 to obtain Z = CH2NR11R12. b This compound is prepared from a compound (III) in which Z = CN and Y = CONR8R9, using the process described in Scheme 9a (step b) to obtain Z = CH2NR11R12. c This compound is prepared from a compound (III) in which Z = CN and Y = CONR8R9, using the process described in Scheme 9a (step c) to obtain Z = CH2NR11R12. d This compound is prepared from a compound (XXXVI) in which Y = CO2R′, using the process described in Scheme 10 to obtain compound (III) with Y = CONR8R9.

Preparation 2.113

(R)2-(N-Boc)amino-3-(4-(3,6-dihydro)-1 (2H)-pyridylmethyl)phenyl)-1-(1,3-thiazol-2-yl)-1-propanone

[0481]

318

A) 2-(N-Boc)amino-3-(4-cyanophenyl)-N-methoxy-N-methylpropanamide

[0482] 2 g of (N-Boc)-4-cyanophenylalanine, 2.32 g of TBTU, 2.40 ml of DIPEA and 806 mg of N,O-dimethylhydroxylamine hydrochloride are mixed together in 30 ml of DCM. After stirring for 24 hours at RT, the reaction mixture is concentrated to dryness and the residue is then extracted with EtOAc. The organic phase is washed with a pH 2 buffer solution, a saturated NaHCO3 solution and then a saturated NaCl solution. The expected compound crystallizes from a mixture of isopropyl ether and pentane (1.89 g).

B) 4-(2-(N-Boc)amino-3-oxo-3-(1,3-thiazol-2-yl)propyl)benzonitrile

[0483] A solution of 1,3-thiazole in 50 ml of THF at −78° C. is prepared, to which are added dropwise 9.57 ml of 2.5 M butyllithium in hexane and then the compound from the preceding step dissolved in 25 ml of THF. After stirring for 1 hour at −78° C., 50 ml of pH 2 buffer solution are added and the mixture is stirred for a further 1 hour at RT. The reaction medium is extracted with EtOAc and the organic phase is then washed with a pH 2 buffer and then with saturated NaCl solution. The mixture is evaporated to dryness and the residue is then chromatographed on silica, eluting with a heptane/acetone mixture (8/2; v/v) to give 1.65 g of the expected compound.

C) 4-(2-(N-Boc)amino)-3-oxo-3-(1,3-thiazol-2-yl)propyl)benzaldehyde

[0484] 1.65 g of the compound from the preceding step, 3 g of Raney nickel and 14 g of NaH2PO2.H2O are placed in 100 ml of a pyridine/acetic acid/water mixture (2/1/1; v/v/v) and the mixture is heated at 55° C. for 3 hours. The nickel is removed by decantation and the medium is then concentrated. The reaction medium is extracted with EtOAc and the organic phase is then washed with a pH 2 buffer, a saturated NaHCO3 solution and saturated NaCl solution. The resulting solution is evaporated to dryness and the residue is then chromatographed on silica, eluting with a heptane/AcOEt mixture (6/4; v/v) to give 400 mg of the expected compound.

D) (R)-2-(N-Boc)amino-3-(4-(3,6-dihydro)-1 (2H)-pyridylmethyl)phenyl)-1-(1,3-thiazol-2-yl)-1-propanone

[0485] 400 mg of the compond from the preceding step, 105 μl of 1,2,3,6-tetrahydropyridine and 3.65 mg of NaBH(OAc)3 are placed in 20 ml of DCE and the mixture is stirred overnight at RT. The reaction mixture is concentrated to dryness and the residue is then taken up in a pH 2 buffer and washed with ether. The acidic phase is extracted with DCM and then washed with saturated NaHCO3 solution. 0.283 g of the expected compound is obtained.

Preparation 2.114

2-(N-Boc)amino-N-isopropyl-N-methyl-3-(4-(1-piperidylmethyl)phenyl)propanethioamide

[0486]

319

[0487] 1.33 g of the compound from Preparation 2.15, step C are placed in 40 ml of anhydrous toluene and the solution is treated with 1.29 g of Lawesson's reagent at 80° C., under argon, for 4 hours. The reaction medium is concentrated and then chromatographed on silica, eluting with a DCM/MeOH/NH4OH mixture (100/4/0.3; v/v/v) to give 0.185 g of the expected compound.

Preparation 3.1

[0488]

320

A) 3-Phenylaminopropionic acid, TFA

[0489] 2 g of tert-butyl 3-phenylaminopropionate are placed in 30 ml of TFA and 20 ml of CH2Cl2, the mixture is stirred for 4 hours at RT and then concentrated to dryness.

B)

[0490] The compound obtained in the preceding step is mixed, at 0° C., in 30 ml of DCM, with 3.80 g of 2-amino-3-(4-((diethylamino)methyl)phenyl)-N-isopropyl-N-methylpropanamide and 5.02 ml of TEA, 4 g of BOP are added and the mixture is then stirred for 4 hours at RT and concentrated to dryness. The residue is extracted with ether and washed with water, with saturated NaHCO3 solution and then with saturated NaCl solution. The resulting solution is dried over sodium sulphate and then chromatographed on silica, eluting with DCM/MeOH (95/5; v/v)+3 ml of NH4OH per litre of eluent. 1.924 g of the expected compound are obtained.

[0491] NMR (DMSO+TFA): 0.8-1.2 ppm: mt: 12H; 2.3-3.0 ppm: mt: 11H; 3.3 ppm: unres.: 2H; 4.0 ppm: mt: 1H; 4.2 ppm: s: 2H; 4.4 ppm: mt: 1H; 7.0-7.4 ppm: mt: 9H.

Preparation 3.2

[0492]

321

[0493] 1.11 g of 3-(N-Boc)amino-3-(benzo[1,3]dioxol-5-ylpropionic acid, 1.508 g of 2-amino-3-(4-(diethylaminoethyl)phenyl)-1-(pyrrolidin-1-yl)propan-1-one (Preparation 2.8), 1.60 g of BOP and 1.50 ml of TEA are placed in 20 ml of DCM and stirred for 2 hours at RT. After concentrating to dryness, the residue is taken up in EtOAc and washed with water, with saturated NaHCO3 solution and then with saturated NaCl solution. The resulting solution is dried over sodium sulphate and concentrated. The residue is triturated in pentane and the precipitate formed is filtered off by suction. The product is chromatographed on silica, eluting with a DCM/MeOH mixture (95/5; v/v)+4 ml of NH4OH per litre of eluent. 1.402 g of the expected compound are obtained.

B)

[0494] 1.4 g of the compound from the preceding step are placed in 15 ml of TFA and 20 ml of DCM and stirred for 2 hours at RT. The reaction mixture is concentrated to dryness and then taken up in DCM and washed with 1N NaOH solution. The solution is dried over sodium sulphate and concentrated. The oil obtained is crystallized in a refrigerator.

[0495] NMR: 0.8-1.1 ppm: mt: 12H; 2.2-2.6 ppm: mt: 9H; 2.8 ppm: mt: 2H; 3.5 ppm: s: 2H; 4.1 ppm: t: 1H; 4.5-5.0 ppm: mt: 2H; 6.0 ppm: s: 2H; 6.8-7.3 ppm: mt: 7H; 8.5 ppm: mt: 1H.

[0496] By working as described in Preparations 1, 2 and 3 above, the compounds of formula (IV), (V) or (VII) collated below are prepared:

7TABLE VII(IV) or (V) or (VII) or (IX)

322

PreparationsX′R2R3Y′Z′3.3 2HClHH

323

324

325

3.4

326

327

328

CHO3.5

329

330

331

CHO3.6

332

333

CO2H

334

3.7

335

336

337

CHO

Preparation 3.3

[0497] NMR (DMSO+TFA): 1.2 ppm: mt: 6H; 1.6 ppm: unres.: 4H; 2.4-3.2 ppm: mt: 12H; 4.2 ppm: s: 2H; 4.5 ppm: mt: 2H; 7.0-7.5 ppm: mt: 9H.

Preparation 3.4

[0498] NMR: 2.2ppm: t: 2H; 2.4ppm: s: 3H; 2.8-3.1: mt: 2H; 3.7ppm: t: 2H; 5.0 ppm: unres.: 1H; 5.9 ppm: s: 2H; 6.4: dd: 1H; 6.6 ppm: d: 1H; 6.7 ppm d: 1H; 7.1-7.5 ppm: mt: 6H; 7.7 ppm: d: 2H; 8.4 ppm: mt: 3H; 9.9 ppm: s: 1H

Preparation 3.5

[0499] NMR: 2.2 ppm: t: 2H; 2.4 ppm: s: 3H; 2.8-3.1 ppm: mt: 2H; 3.8 ppm: t: 2H; 5.0 ppm: mt: 1H; 6.1: d: 1H; 6.3 ppm: mt: 1H; 7.0-7.6 ppm: mt: 10H; 7.7 ppm; d: 2H; 8.3 ppm: d: 1H; 9.9 ppm: s: 1H.

Preparation 3.7

NMR: 2.2 ppm: t: 2H; 2.4 ppm: s: 3H; 2.7-3.1 ppm: mt: 2H; 3.8 ppm: t: 2H; 5.0 ppm: mt: 1H; 7.0-7.8 ppm: mt: 13H; 8.3-8.5 ppm: mt: 3H; 9.9 ppm

Preparation 3.8

[0500]

338

[0501] A mixture containing 480 mg of the compound from Preparation 2.41, 259 mg of the compound from Preparation 1.13, 370 mg of BOP and 440 μl of DIPEA in 10 ml of DCM is stirred at RT for 3 hours. The medium is concentrated to dryness and is then taken up in EtOAc, washed with water and extracted with pH 2 buffer, and the aqueous phase is then basified to pH 8 with saturated NaHCO3 solution. This mixture is extracted with DCM and then washed with saturated NaCl solution to give 0.437 g of the expected compound.

[0502] NMR: 0.5 to 1.6 ppm: unres.: 27H; 2.2 to 3.6 ppm: unres.: 10H; 3.8 to 5 ppm: unres.: 4H; 5.9 ppm: d: 2H; 6.6 to 7.3 ppm: unres.: 8H; 8.1 ppm: t: 1H.

B) 437 mg of the compound obtained in the preceding step and 10 ml of TFA in 20 ml of DCM are stirred for 4 hours at RT. The mixture is concentrated to dryness and then crystallized from ethyl ether/pentane mixture. The residue is taken up in a DCM/saturated NaHCO3 solution mixture; the organic phase is dried and concentrated to dryness under vacuum to give 343 mg of the expected compound.

Preparation 3.9

[0503]

339

[0504] 163 mg of the compound obtained in Preparation 2.42 are placed in 5 ml of DCM with 3 ml of TFA and are stirred for 1 hour at RT. The medium is concentrated and then triturated in iPr2O and decanted to recover the oil formed. The oil formed is taken up in 10 ml of DCM, 115 mg of the compound from Preparation 1.13 and 170 mg of BOP are then added and the mixture is maintained at pH 7 by addition of DIPEA. After the usual work-up, 0.225 g of the expected compound is obtained, which is used in crude form in the following step.

Preparation 3.10

[0505]

340

[0506] This compound is prepared according to the methods described above, starting with the compound from Preparation 2.43 and the compound from Preparation 1.13.

EXAMPLE 1

N-[1-(4-Aminomethylbenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0507]

341

A) N-[1-(4-Cyanobenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0508] 0.25 ml of triethylamine is added to a mixture of 0.715 g of the compound from Preparation 2.1 in 15 ml of acetonitrile, followed by addition of 0.71 g of the compound from Preparation 1.1 and 0.45 g of DCC, and the mixture is stirred for 5 hours at RT. The reaction mixture is concentrated under vacuum, the residue is taken up in acetone, the DCU is filtered off and the filtrate is concentrated under vacuum. The residue is triturated in ether and the solvent is then decanted off (several times). 0.61 g of the expected product is obtained after drying under vacuum over P2O5; m.p.=195-200° C.

[0509] NMR: δ (ppm): 1.40-170: unres.: 4H; 2.30-3.40: unres.: 8H; 4.40-4.60: unres.: and 4.60-4.70: unres.: 2H; 6.75-8.15: unres.: 16H.

B) N-[1-(4-Aminomethylbenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0510] 1 g of the compound obtained in the preceding step is dissolved in 4.5 ml of concentrated aqueous ammonia, 20 ml of MeOH, 5 ml of dioxane and 20 ml of toluene, 0.9 g of Raney® nickel is added and the mixture is hydrogenated at 50° C. under a pressure of 50 bar for 7 hours. The catalyst is filtered off through Celite® and washed with MeOH/chloroform solution, and the filtrate is concentrated under vacuum. The residue is taken up in ether and the precipitate formed is filtered off by suction. The product obtained is taken up in hydrochloric ether and the precipitate formed is filtered off by suction and dried. 0.85 g of the expected product is obtained. MH+=585

EXAMPLE 2

N-[1-[4-(tert-Butylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0511]

342

[0512] 1.46 ml of DIPEA are added to a mixture of 1.06 g of the compound obtained in Preparation 1.1 in 15 ml of DMF, followed by addition of 1.49 g of the compound obtained in Preparation 2.2 and 1.38 g of BOP, and the mixture is stirred for 2 hours 30 minutes at RT, while maintaining the pH at 6 by adding DIPEA. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with 0.2N NaOH, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH/NH4OH mixture (85/15/0.2; v/v/v). The product obtained is dissolved in 10 ml of MeOH, 0.2 ml of 10N HCl is added and the mixture is concentrated under vacuum. The residue is chromatographed on Sephadex® LH20, eluting with a DCM/MeOH mixture (60/40; v/v). 0.66 g of the expected product is obtained.

[0513] NMR: δ (ppm): 1.3: s: 9H; 1.5-1.9: unres.: 4H; 2.3-3.4: unres.: 8H; 3.95: d: 2H; 4.4-4.9: unres.: 2H; 6.8-8.6: unres.: 18H.

EXAMPLE 3

N-[1-[4-(N-Propyl-N-methylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0514]

343

[0515] 0.458 ml of triethylamine is added to a mixture of 0.39 g of the compound obtained in Preparation 1.1 and 0.41 g of the compound obtained in Preparation 2.3 in 10 ml of DCM, followed by addition of 0.533 g of BOP, and the mixture is stirred for 4 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water, with saturated NaCl solution, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.3 g of the expected product is obtained after crystallization from ether.

[0516] NMR: δ (ppm): 0.75 t: 3H; 1.2-1.7: unres.: 6H; 2.0: s: 3H; 2.15: t: 2H; 2.25-3.5: unres.: 10H; 4.1 to 4.8: unres.: 2H; 6.7 to 8.5: unres.: 18H.

EXAMPLE 4

N-[1-[4-[N,N-bis(2-hydroxyethyl)aminomethyl]benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0517]

344

[0518] 1.3 ml of DIPEA are added to a mixture of the compound obtained in Preparation 2.4 in 15 ml of DMF, followed by addition of 0.96 g of the compound obtained in Preparation 1.1 and 1.33 g of BOP, and the mixture is stirred for 2 hours at RT, while maintaining the pH at 6 by adding DIPEA. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with 0.5N NaOH, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH/NH4OH mixture (90/10/0.2; v/v/v). The product obtained is chromatographed on Sephadex® LH 20, eluting with a DCM/MeOH mixture (60/40; v/v). 0.44 g of the expected product is obtained.

[0519] NMR: δ (ppm): 1.3 to 1.7: unres.: 4H; 2.2 to 3.6: unres.: 18H; 4.2 to 4.8: unres.: 4H; 6.8 to 8.4: m: 18H.

EXAMPLE 5

N-[1,2-bis[4-(diethylaminomethyl)phenyl]ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide.

[0520]

345

[0521] 0.8 ml of DIPEA is added to a mixture of 0.82 g of the compound obtained in Preparation 2.5 in 10 ml of DMF, followed by addition of 0.39 g of the compound obtained in Preparation 1.1 and 0.53 g of the BOP, and the mixture is stirred for 1 hour 30 minutes at RT, while maintaining the pH at 6 by adding DIPEA. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with 0.1N NaOH, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on Sephadex® LH 20, eluting with a DCM/MeOH mixture (60/40; v/v). 0.32 g of the expected product is obtained.

[0522] NMR: δ (ppm): 0.85: t: 12H; 2.1 to 3.6: unres.: 16H; 4.4 to 4.9: unres.: 2H; 6.6 to 8.4: unres.: 22H.

EXAMPLE 6

N-[1-[4-(diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-[methyl-(naphthalene-2-sulphonyl)amino]-3-phenylpropionamide

[0523]

346

[0524] 1 ml of DIPEA is added to a mixture of 0.72 g of the compound obtained in Preparation 2.7 in 6 ml of DMF, followed by addition of 0.51 g of the compound obtained in Preparation 1.2 and 0.67 g of BOP, and the mixture is stirred for 3 hours at RT while maintaining the pH at 6 by adding DIPEA. The reaction mixture is extracted with EtOAc, the organic phase is washed with water, with 0.5N NaOH, with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH/NH4OH mixture (90/10/0.2; v/v/v). 0.27 g of the expected product is obtained.

[0525] NMR: δ (ppm): 0.95: t: 6H; 1.35 to 1.8: unres.: 4H; 2.1 to 3.6: unres.: 12H; 4.0 to 4.6: unres.: 3H; 5.55: mt: 1H; 6.8 to 8.6: unres.: 17H.

EXAMPLE 7

N-[2-[4-(Diethylaminomethyl)phenyl)-1-(N-isopropyl-N-methylcarbamoyl)ethyl]-3-(3,4-dimethylphenyl)-3-(naphthalene-2-sulphonylamino) propionamide hydrochloride

[0526]

347

[0527] 0.312 ml of triethylamine is added to a mixture of 0.288 g of the compound obtained in Preparation 1.4 and 0.4 g of the compound obtained in Preparation 2.8 in 15 ml of DCM and 3 ml of DMF, followed by addition of 0.332 g of BOP, and the mixture is stirred for 2 hours at RT. The reaction mixture is diluted with DCM and the organic phase is washed with 1N NaOH and with water and concentrated under vacuum. The residue is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is dissolved in acetonitrile, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.263 g of the expected product is obtained.

[0528] NMR: δ (ppm): 0.6-1.3: unres.: 12H; 1.6-1.8: unres.: 6H; 2.3-3.2: unres.: l1H; 3.7-4.9: unres.: 5H; 6.4-8.4: unres.: 16H.

EXAMPLE 8

N-[2-[4-(Diethylaminomethyl)phenyl]-1-(N-isopropyl-N-methylcarbamoyl)ethyl]-3-(benzo[1,3]dioxol-5-yl)-3-(naphthalene-2-sulphonylamino) propionamide hydrochloride

[0529]

348

[0530] 0.996 ml of triethylamine and 1.16 g of BOP are added to a mixture of 0.95 g of the compound obtained in Preparation 1.7 and 1.27 g of the compound obtained in Preparation 2.8 in 10 ml of DCM, and the mixture is stirred for 3 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (93/7; v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and concentrated under vacuum. 0.78 g of the expected product is obtained after crystallization from an EtOAc/iso ether mixture.

[0531] NMR: δ (ppm). 0.6 to 1.4: unres.: 12H; 2.2 to 3.2: unres.: 1H; 3.7 to 5.0: unres.: 5H; 5.2 to 5.8: unres.: 2H; 6.2 to 8.5: unres.: 16H; 10.4: s: 1H.

EXAMPLE 9

N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-3-(naphthalene-2-sulphonylamino)propionamide hydrochloride

[0532]

349

[0533] 0.497 ml of triethylamine and 0.529 g of BOP are added to a mixture of 0.494 g of the compound obtained in Preparation 1.8 and 0.450 g of the compound obtained in Preparation 2.7 in 10 ml of DCM, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with DCM, the organic phase is washed with water, with 1N NaOH and with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. The product obtained is dissolved in a 2-propanol/acetonitrile mixture, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.280 g of the expected product is obtained.

[0534] NMR: δ (ppm): 1.2: mt: 6H; 1.4 to 1.8: unres.: 12H; 3.6 to 5.0: unres.: 8H; 6.1 to 8.4: unres.: 16H; 10.5: s: 1H.

EXAMPLE 10

N-[2-[4-(Diethylaminomethyl)phenyl]-1-(N-isopropyl-N-methylcarbamoyl)ethyl]-3-(2,3-dihydrobenzo[1,4]dioxin-6-yl)-3-(5,6,7,8-tetrahydronaphthalene-2-sulphonylamino)propionamide hydrochloride

[0535]

350

[0536] 0.312 ml of triethylamine is added to a mixture of 0.312 g of the compound obtained in Preparation 1.9 and 0.4 g of the compound obtained in Preparation 2.8 in 15 ml of DCM and 3 ml of DMF, followed by addition of 0.332 g of BOP, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with a pH 4 buffer solution, with water and with saturated NaHCO3 solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.24 g of the expected product is obtained.

[0537] NMR: δ (ppm): 0.6-1.0: unres.: 6H; 1.05-1.35: unres.: 6H; 1.4 to 1.8: unres.: 4H; 2.2 to 3.1: unres.: 15H; 3.9-5.0: unres.: 9H; 6.2 to 8.4: unres.: 12H.

EXAMPLE 11

N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-[(2,4-dichloro-3-methylbenzenesulphonyl)phenylamino]propionamide hexafluorophosphate

[0538]

351

[0539] This compound is prepared according to the procedure described in Example 3, starting with 0.46 g of the compound obtained in Preparation 2.7 and 0.475 g of the compound obtained in Preparation 1.10 in 10 ml of DCM and 0.511 ml of triethylamine, followed by 0.595 g of BOP. 0.35 g of the expected product is obtained after crystallization from ether; m.p.=204-208° C.

[0540] NMR: δ (ppm): 1.05: t: 6H; 1.4 to 1.8: unres.: 4H; 2.2: t: 2H; 2.4: s: 3H; 2.5 to 3.5: unres.: 10 H; 3.6 to 4.6: unres.: 5H; 6.8 to 7.7: unres.: 11H; 8.3: d: 1H; 10.4: bs: 1H.

EXAMPLE 12

N-[2-[4-(Diethylaminomethyl)phenyl]-1-(pyrid-3-yl)ethyl]-3-[(2,4-dichloro-3-methylbenzenesulphonyl)phenylamino]propionamide dihydrochloride

[0541]

352

[0542] This compound is prepared according to the procedure described in Example 8, starting with the crude compound obtained in Preparation 2.10, 0.454 g of the compound obtained in Preparation 1.10 in 20 ml of DCM, 0.326 ml of triethylamine and 0.518 g of BOP. The product obtained is chromatographed on silica gel, eluting with a DCM/MeOH/2% NH4OH mixture (95/5/0.5; v/v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and concentrated under vacuum. 0.5 g of the expected product is obtained after crystallization from an EtOAc/iso ether mixture; m.p.=155° C. (decomposition).

[0543] NMR: δ (ppm): 1.1: t: 6H; 2.2: mt: 2H; 2.4: s: 3H; 2.7 to 3.2: unres.: 6H; 3.8: t: 2H; 4.1: bd: 2H; 5.1: q: 1H; 6.8 to 9.0: unres.: 16H; 10.8: s: 1H.

EXAMPLE 13

N-[1-[4-(N-ethyl-N-methylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0544]

353

A) Ethyl 3-[4-(N-ethyl-N-methylaminomethyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-phenylpropionylamino]propionate

[0545] 0.791 g of BOP and 0.226 ml of triethylamine are added to a mixture of 0.578 g of the compound obtained in Preparation 1.1 and 0.430 g of the compound obtained in Preparation 2.11 in 10 ml of DCM, and the mixture is stirred for 3 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (100/4; v/v). 0.5 g of the expected product is obtained.

B) 3-[4-(N-Ethyl-N-methylaminomethyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-phenylpropionylamino]propionic acid

[0546] 1.7 ml of 1N KOH are added to a mixture of 0.5 g of the compound obtained in the preceding step in 10 ml of EtOH and 10 ml of dioxane, and the mixture is heated at 60° C. for 4 hours. After cooling to RT, 1.7 ml of 1N HCl are added and the mixture is concentrated under vacuum. The residue is taken up in EtOH and concentrated again under vacuum. The expected product is obtained, and is used without further purification.

C) N-[1-[4-(N-Ethyl-N-methylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0547] 0.403 g of BOP and 0.115 ml of triethylamine are added to a mixture of the compound obtained in the preceding step and 0.07 ml of pyrrolidine in 10 ml of DMF, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into water and extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.4 g of the expected product is obtained after crystallization from iso ether.

[0548] NMR: δ (ppm): 1.1: t: 3H; 1.3 to 1.85: unres.: 4H; 2.1: s: 3H; 2.2 to 3.5: unres.: 12H; 4.2 to 5.0: unres.: 2H; 6.8 to 8.6: unres.: 18H.

EXAMPLE 14

N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0549]

354

A) Ethyl 3-[4-(diethylaminomethyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-phenylpropionylamino]propionate

[0550] A mixture of 0.599 g of the compound obtained in Preparation 1.11, 0.368 g of the compound obtained in step B of Preparation 2.7 and 0.184 ml of triethylamine in 10 ml of DMF is stirred for 4 hours at RT. After concentrating the reaction mixture under vacuum, the residue is taken up in EtOAc, the organic phase is washed with water and with saturated NaHCO3 solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.715 g of the expected product is obtained.

B) 3-[4-(Diethylaminomethyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-phenylpropionylamino]propionic acid

[0551] 1.75 ml of 1N KOH are added to a mixture of 0.715 g of the compound obtained in the preceding step in 10 ml of EtOH and 10 ml of dioxane, and the mixture is heated overnight at 60° C. 1.75 ml of 1N HCl are added and the mixture is concentrated under vacuum. The expected product is obtained, and is used without further purification.

C) N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0552] 0.161 ml of triethylamine and 0.515 g of BOP are added to a mixture of the compound obtained in the preceding step and 0.098 ml of pyrrolidine in 10 ml of DMF, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into water and extracted with EtOAc, the organic phase is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.618 g of the expected product is obtained.

[0553] NMR: δ (ppm): 0.8 to 1.0: t: 6H; 1.3 to 1.7: unres.: 4H; 2.2 to 3.5: unres.: 14H; 4.1 to 4.8: unres.: 2H; 6.7 to 8.4: unres.: 18H.

EXAMPLE 15

N-[2-[4-(Diethylaminomethyl)phenyl]-1-(N-ethyl-N-isopropylcarbamoyl)ethyl]-3-(benzo[1,3]dioxol-5-yl)-3-(naphthalene-2-sulphonylamino)propionamide hydrochloride

[0554]

355

A) Ethyl 3-[4-(diethylaminomethyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-(benzo[1,3]dioxol-5-yl)propionylamino]propionate

[0555] 0.652 g of BOP and 0.205 ml of triethylamine are added to a mixture of 0.589 g of the compound obtained in Preparation 1.7 and 0.410 g of the compound obtained in step B of Preparation 2.7 in 10 ml of DMF, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into an EtOAc/saturated NaHCO3 mixture, the organic phase is washed with water and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.839 g of the expected product is obtained.

B) 3-[4-(Diethylaminometbyl)phenyl]-2-[3-(naphthalene-2-sulphonylamino)-3-(benzo[1,3]dioxol-5-yl)propionylamino]propionic acid

[0556] 1.9 ml of 1N KOH are added to a mixture of 0.83 g of the compound obtained in the preceding step in 7 ml of MeOH, 7 ml of EtOH and 7 ml of dioxane, and the mixture is stirred for 4 hours at RT. 1.9 ml of 1N HCl are added and the mixture is concentrated under vacuum. The residue is taken up in an EtOH/MeOH mixture (50/50; v/v), concentrated under vacuum, taken up in toluene and concentrated under vacuum again. 1.204 g of the expected product are obtained.

C) N-[2-[4-(Diethylaminomethyl)phenyl]-1-(N-ethyl-N-isopropylcarbamoyl)-ethyl]-3-(benzo[1,3]dioxol-5-yl)-3-(naphthalene-2-sulphonylamino)propionamide hydrochloride

[0557] 0.46 g of bromotripyrrolidinophosphonium hexafluorophosphate and 0.33 ml of DIPEA are added to a mixture of 0.6 g of the compound obtained in the preceding step and 0.126 ml of N-ethylisopropylamine in 15 ml of DCM, and the mixture is stirred for 24 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water, with saturated NaHCO3 solution and with saturated NaCl solution, the organic phase is extracted with a pH 4 buffer solution, the aqueous phase is basified by adding saturated NaHCO3 solution and extracted with DCM, the organic phase is washed with saturated NaHCO3 solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (95/5; v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.058 g of the expected product is obtained.

[0558] NMR: δ (ppm): 0.6-1.4: unres.: 15H; 2.2-3.3: unres.: 10H; 3.7-4.9: unres.: 5H; 5.3-5.8: unres.: 2H; 6.3-8.5: unres.: 16H; 10.5: s: 1H.

EXAMPLE 16

N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-phenyl-3-(quinoline-2-sulphonylamino)propionamide dihydrochloride

[0559]

356

A) Ethyl 3-[4-(diethylaminomethyl)phenyl]-2-[3-phenyl-3-(quinoline-2-sulphonylamino)propionylamino]propionate

[0560] 0.467 g of BOP is added to a mixture of 0.376 g of the compound obtained in Preparation 1.12 and 0.421 g of the compound obtained in step B of Preparation 2.7 in 10 ml of DMF, followed by addition of 0.147 ml of triethylamine, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into an NaHCO3 solution and extracted with EtOAc, the extracts are washed with saturated NaHCO3 solution and extracted with 1N HCl, the acidic aqueous phase is basified by adding solid NaHCO3 and extracted with EtOAc, the organic phase is washed with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.455 g of the expected product is obtained.

B) 3-[4-(Diethylaminomethyl)phenyl]-2-[3-phenyl-3-(quinoline-2-sulphonylamino)propionylamino]propionic acid

[0561] 1.63 ml of 1N NaOH are added to a mixture of 0.455 g of the compound obtained in the preceding step in 20 ml of EtOH and 5 ml of dioxane, and the mixture is then stirred overnight at RT. 1.63 ml of 1N HCl are added and the mixture is concentrated under vacuum. The residue is taken up in toluene and concentrated again under vacuum. The expected product is obtained, and is used without further purification.

C) N-[1-[4-(Diethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-phenyl-3-(quinoline-2-sulphonylamino)propionamide dihydrochloride

[0562] 0.103 ml of triethylamine is added to a mixture of the compound obtained in the preceding step and 0.063 ml of pyrrolidine in 10 ml of DMF, followed by addition of 0.327 g of BOP, and the mixture is stirred for 2 hours at RT. The reaction mixture is poured into an EtOAc/saturated NaHCO3 mixture, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (95/5; v/v) and then with a DCM/MeOH/NTH4OH mixture (90/10/0.4; v/v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.086 g of the expected product is obtained.

[0563] NMR: δ (ppm): 1.0 to 1.9: unres.: 10H; 2.45 to 3.55: unres.: 12H; 4.0 to 5.0: unres.: 4H; 6.8 to 8.9: unres.: 17H; 10.1: s: 1H.

EXAMPLE 17

N-[1-[4-(Ethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0564]

357

A) N-[1-[4-Formylbenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0565] 3 g of aluminium/nickel alloy are added to a mixture of 3 g of the compound obtained in step A of Example 1 and 40 ml of 75% formic acid, and the mixture is refluxed for 2 hours. The insoluble material is filtered off by suction while hot and washed with MeOH, and the filtrate is concentrated under vacuum. The residue is taken up in chloroform, the insoluble material is filtered off by suction and washed with an MeOH/chloroform mixture, and the filtrate is concentrated under vacuum. The residue is taken up in chloroform and filtered, the filtrate is washed with saturated NaHCO3 solution and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. 2.4 g of the expected product are obtained.

B) N-[1-[4-(Ethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0566] AcOH is added, to pH 5, to a mixture of 0.5 g of the compound obtained in the preceding step, 0.057 g of sodium cyanoborohydride, 0.077 g of ethylamine hydrochloride and 0.119 ml of triethylamine in 15 ml of MeOH, and the mixture is stirred for 2 hours at RT. The mixture is concentrated under vacuum, the residue is extracted with DCM, the organic phase is washed with saturated NaHCO3 solution, with KHSO4/K2SO4 buffer solution, with saturated NaHCO3 solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (90/10; v/v) and then a DCM/MeOH/NH4OH mixture (90/10/0.4; v/v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.112 g of the expected product is obtained after crystallization from an MeOH/ether mixture.

[0567] NMR: δ (ppm): 1.0 to 1.9: unres.: 7H; 2.3 to 3.4: unres.: 10H; 4.0: s: 2H; 4.25 to 4.8: unres.: 2H; 6.6 to 8.2: unres.: 14H.

EXAMPLE 18

N-[1-[4-(Dimethylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0568]

358

[0569] 1 ml of AcOH and 0.129 g of dimethylamine hydrochloride are added to a solution of 0.830 g of the compound obtained in step A of Example 17 in 10 ml of DCM, followed by portionwise addition of 0.456 g of sodium triacetoxyborohydride, and the mixture is stirred for 18 hours at RT. The reaction mixture is diluted with DCM, the organic phase is washed with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (95/5; v/v). 0.52 g of the expected product is obtained.

[0570] MH+=613

EXAMPLE 19

N-[1-[4-(Azetidin-1-ylmethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0571]

359

[0572] A mixture of 0.489 g of the compound obtained in step A of Example 17 and 0.086 g of azetidine hydrochloride and 116 μl of TEA in 15 ml of MeOH is stirred for 1 hour at RT. Next, 0.072 ml of AcOH is added, the mixture is stirred for 15 minutes at RT, 0.079 g of sodium cyanoborohydride is added and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with DCM, the organic phase is washed with saturated NaHCO3 solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a DCM/MeOH mixture (95/5; v/v) and then a DCM/MeOH/NH4OH mixture (95/5/0.5; v/v/v). The product obtained is dissolved in EtOAc, acidified to pH 1 by adding hydrochloric ether and diluted with ether, and the precipitate formed is filtered off by suction. 0.115 g of the expected product is obtained.

[0573] NMR: δ (ppm): 1.4-1.8: unres.: 4H; 2.1 to 3.25: unres.: 10H; 3.6-4.8: unres.: 8H; 6.8-8.5: unres.: 18H; 10.85: s: 1H.

EXAMPLE 20

N-[1-[4-(dibutylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0574]

360

A) N-[1-[4-(Hydroxymethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamnide

[0575] 0.57 g of sodium nitrite is added to a mixture of 3.5 g of the compound obtained in Example 1 in 100 ml of water and 20 ml of dioxane, and the mixture is heated at 110° C. for 2 hours. The reaction mixture is concentrated under vacuum, the residue is extracted with EtOAc, the organic phase is washed with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is taken up in ether and the precipitate formed is filtered off by suction. 2.78 g of the expected product are obtained.

B) N-[1-[4-(Chloromethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide and N-[1-[4-(methylsulphonyloxymethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0576] 0.3 ml of triethylamine is added to a solution of 1 g of the compound obtained in the preceding step in 10 ml of DCM, followed by addition of 0.167 ml of methanesulphonyl chloride, and the mixture is stirred for 30 minutes at RT. A further 0.3 ml of triethylamine is added, followed by addition of 0.167 ml of methanesulphonyl chloride and the reaction mixture is stirred for 30 minutes and concentrated under vacuum. The residue is extracted with EtOAc, the organic phase is washed with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. 0.68 g of the mixture of expected products is obtained.

C) N-[1-[4-(dibutylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-naphthalene-2-sulphonylamino)-3-phenylpropionamide

[0577] 0.045 g of 80% sodium hydride in oil is added to a solution of 0.245 g of dibutylamine in 5 ml of THF, and the mixture is stirred for 30 minutes at RT. 1 g of the compound obtained in the preceding step is then added and the mixture is stirred for 18 hours at RT. The reaction mixture is concentrated under vacuum, the residue is extracted with DCM, the organic phase is washed with water and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (95/5; v/v). 0.2 g of the expected product is obtained.

[0578] MH+=698

EXAMPLE 21

N-[1-[4-(Butylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0579]

361

A) N-[1-[4-(N-Benzyl-N-butylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl) ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0580] 0.073 g of 80% sodium hydride in oil is added to a solution of 0.397 g of N-butylbenzylamine in 5 ml of THF, and the mixture is stirred for 30 minutes at RT. 1.5 g of the compound obtained in step B of Example 20 are then added and the mixture is stirred for 18 hours at RT. The insoluble material is filtered off by suction and washed with THF, and the filtrate is concentrated under vacuum. The residue is extracted with DCM, the organic phase is washed with 0.1N HCl and with saturated NaCl solution and dried over Na2SO4, and the solvent is evaporated off under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (96/4; v/v). 0.265 g of the expected product is obtained.

B) N-[1-[4-(Butylaminomethyl)benzyl]-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-(naphthalene-2-sulphonylamino)-3-phenylpropionamide hydrochloride

[0581] 0.6 ml of 1N HCl is added to a solution of 0.47 g of the compound obtained in the preceding step in 5 ml of MeOH, followed by addition of 0.5 g of 10% palladium-on-charcoal, and the mixture is hydrogenated at RT under a pressure of 13 332 Pa for 18 hours. The catalyst is filtered off through Celite® and washed with an MeOH/chloroform mixture, and the filtrate is concentrated under vacuum. The residue is chromatographed on silica gel, eluting with a chloroform/MeOH mixture (95/5; v/v). 0.12 g of the expected product is obtained.

[0582] MH+=641

[0583] By working according to the procedures described in the preceding Examples, the compounds according to the invention collated in Table VIII below are prepared.

8TABLE VIII(I)

362

SaltExamplesR3R4R5mass or NMR22 (a)

363

364

365

NMR23 (b)

366

367

—CH2—N(Et)2HCl NMR24 (c)

368

369

—CH2—N(Et)2NMR25 (d)

370

371

—CH2—N(Et)2NMR26 (e)

372

373

—CH2—N(Et)2NMR27 (f)

374

—CO—N(iPr)2—CH2—N(Et)2HCl NMR28 (g)

375

376

—CH2N(n-Pr)2HCl NMR29 (h)

377

378

—CH2—N(iPr)2HCl NMR30 (i)

379

380

—CH2—N(Et)2HCl NMR31 (j)

381

382

—CH2N(Et)2HCl NMR32 (k)

383

384

—CH2NH—CH2CH2OHMass33 (l)

385

386

387

Mass34 (m)

388

389

390

NMR35 (n)

391

392

393

NMR36 (o)

394

395

396

NMR

[0584] Mass or proton NMR spectra at 200 MHz in DMSO-d6 of the compounds of the Examples of Table I.

EXAMPLE 22

[0585] δ (ppm): 1.2-1.9: unres.: 10H; 2.5-3.4: unres.: 12H; 4.2: s: 2H; 4.45: t: 1H; 4.7: t: 1H; 6.8-8.3: unres.: 16H.

EXAMPLE 23

[0586] δ (ppm): 1.2: t: 6H; 1.4-1.75: unres.: 4H; 1.8: s: 3H; 2.2-3.2: unres.: 12H; 4.0-4.8: unres.: 4H; 6.5-8.4: unres.: 17H; 10.4: s: 1H.

EXAMPLE 24

[0587] δ (ppm): 0.9: t: 6H; 1.3-1.6: unres.: 4H; 2.2-3.5: unres.: 20H; 4.2-4.7: unres.: 2H; 5.85: t: 1H; 6.15: d: 2H; 6.8-8.3: unres.: 13H.

EXAMPLE 25

[0588] δ (ppm): 1.0: t: 6H; 1.4-1.8: unres.: 4H; 2.3-3.7: unres.: 20H; 4.3-4.8: unres.: 2H; 6.4-8.4: unres.: 16H.

EXAMPLE 26

[0589] δ (ppm): 0.5-1.0: unres.: 12H; 2.2-2.9: unres.: 11H; 3.35-5.0: unres.: 9H; 6.2-8.4: unres.: 16H.

EXAMPLE 27

[0590] δ (ppm): 0.5-1.4: unres.: 18H; 2.2-3.1: unres.: 8H; 3.15-4.8: unres.: 10H; 6.2-8.3: unres.: 16H; 10.35: s: 1H.

EXAMPLE 28

[0591] δ (ppm): 0.6-0-9: mt: 6H; 1.3-1.9: mt: 8H; 2.2-3.65: mt: 12H; 4.0-4.9: unres.: 4H; 6.7-8.5: unres.: 18H; 10.5: bs: 1H.

EXAMPLE 29

[0592] δ (ppm): 1.0-1.8: unres.: 16H; 2.2-3.7: unres.: 10H; 4.0-4.8: unres.: 4H; 6.7-8.5: unres.: 18H; 9.2: bs: 1H.

EXAMPLE 30

[0593] δ (ppm): 1.3-1.8: unres.: 4H; 2.2-3.8: unres.: 8H; 3.9-5.0: unres.: 6H; 6.6-8.8: unres.: 22H; 11.35: mt: 1H.

EXAMPLE 31

[0594] δ (ppm): 1.0-1.3: mt: 6H; 1.4-1.8: unres.: 4H; 2.2-3.2: unres.: 12H; 4.0-4.7: unres.: 4H; 5.2-5.7: unres.: 2H; 6.2-8.4: unres.: 16H; 10.2: bs: 1H.

EXAMPLE 32

[0595] MH+=629

EXAMPLE 33

[0596] MH+=639

EXAMPLE 34

[0597] δ (ppm): 0.5-1.0: unres.: 6H; 1.25-1.65: unres.: 6H; 2.1-3.0: unres.: 11H; 3.2-4.9: unres.: 5H; 5.2-5.8: unres.: 2H; 6.2-8.4: unres.: 16H.

EXAMPLE 35

[0598] δ (ppm): 0.4-0.9: unres.: 6H; 1.15-2.1: unres.: 11H; 2.15-2.9: unres.: 8H; 3.2-4.8: unres.: 5H; 5.2-5.7: mt: 2H; 6.2-8.3: unres. 16H.

EXAMPLE 36

[0599] δ (ppm): 0.4-0.8: unres.: 6H; 1.05-2.65: unres.: 4H; 2.7-2.9: unres.: 11H; 3.2-4.8: unres.: 7H; 5.2-5.65: unres.: 2H; 6.2-8.3: unres.: 16H.

[0600] (a) This compound is prepared according to the procedure described in Example 3, starting with the compound obtained in Preparation 1.1 and the compound obtained in Preparation 2.6.

[0601] (b) This compound is prepared according to the procedure described in Example 3, starting with the compound obtained in Preparation 1.3 and the compound obtained in Preparation 2.7. The crude product obtained is dissolved in EtOAc and acidified to pH 1 by adding hydrochloric ether, diluted with ether, and the precipitate formed is filtered off by suction.

[0602] (c) This compound is prepared according to the procedure described in Example 3, starting with the compound obtained in Preparation 1.5 and the compound obtained in Preparation 2.7.

[0603] (d) This compound is prepared according to the procedure described in Example 3, starting with the compound obtained in Preparation 1.6 and the compound obtained in Preparation 2.7.

[0604] (e) This compound is prepared according to the procedure described in Example 7, starting with the compound obtained in Preparation 1.8 and the compound obtained in Preparation 2.8, without preparing the hydrochloride.

[0605] (f) This compound is prepared according to the procedure described in Example 8, starting with the compound obtained in Preparation 1.8 and the compound obtained in Preparation 2.9.

[0606] (g) This compound is prepared according to the procedure described in Example 13, step A, starting with the compound obtained in Preparation 1.1 and the compound obtained in Preparation 2.12, followed by saponification with 1N KOH according to step B and coupling with pyrrolidine according to step C. At the end of step C, formation of the hydrochloride in EtOAc by addition of hydrochloric ether.

[0607] (h) This compound is prepared according to the procedure described in Example 13, step A, starting with the compound obtained in Preparation 1.1 and the compound obtained in Preparation 2.13, followed by saponification with 1N KOH according to step B and coupling with pyrrolidine according to step C. Formation of the hydrochloride according to (g) above.

[0608] (i) This compound is prepared according to the procedure described in step C of Example 13, starting with the compound obtained in step B of Example 15 and N-methylcyclopentylamine. The product obtained is chromatographed on silica gel, eluting with a DCM/MeOH/NH4OH mixture (95/5/0.4; v/v/v) and the hydrochloride is then formed in EtOAc/hydrochloric ether.

[0609] (j) This compound is prepared according to the procedure described in step C of Example 13, starting with the compound obtained in step B of Example 15 and pyrrolidine. The product obtained is chromatographed on silica gel, eluting with a DCM/MeOH/NH4OH mixture (95/5/0.4; v/v/v) and the hydrochloride is then formed in EtOAc/hydrochloric ether and crystallized from MeOH/ether.

[0610] (k) This compound is prepared according to the procedure described in Example 17, starting with the compound obtained in step A of Example 18 and 2-aminoethanol.

[0611] (l) This compound is prepared according to the procedure described in Example 18, starting with the compound obtained in step A of Example 17 and pyrrolidine.

[0612] (m) This compound is prepared according to the procedure described in Example 8, starting with the compound obtained in Preparation 1.7 and the compound obtained in Preparation 2.15, without performing chromatography or salification.

[0613] (n) This compound is prepared according to the procedure described in Example 8, starting with the compound obtained in Preparation 1.7 and the compound obtained in Preparation 2.16, without performing chromatography or salification.

[0614] (o) This compound is prepared according to the procedure described in Example 8, starting with the compound obtained in Preparation 1.7 and the compound obtained in Preparation 2.17, without performing chromatography or salification.

EXAMPLE 37

[0615]

397

A) (R) N-[1-(4-Cyanobenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-[N-(benzo[1,3]dioxol-5-yl)amino]propionamide

[0616] This compound is obtained by the action of the compound from Preparation 1.17 on the compound from Preparation 2.18.

B) (R) N-[1-(4-formylbenzyl)-2-oxo-2-(pyrrolidin-1-yl)ethyl]-3-[N-(benzo[1,3]dioxol-5-yl)amino]propionamide

[0617] 550 mg of the compound from the preceding step are placed in 16 ml of a pyridine/acetic acid/water mixture (2/1/1) at 0° C., and 1.3 g of NaH2PO2.H2O and 260 mg of Raney nickel are added. The reaction medium is heated for 2 hours at 55° C. and then filtered through Celite® and rinsed with an EtOH/DCM mixture (1/1), and the filtrate is concentrated. The residue is extracted with EtOAc and then washed successively with water (twice), with saturated NaHCO3 solution, with water, with 5% KHSO4 solution and with saturated NaCl solution. The expected product is obtained, and is used without further purification in the following step. 126

[0618] C) The product obtained in the preceding step is placed in 10 ml of dichloromethane and is treated with 160 μl of methyl-tert-butylamine and 264 mg of NaHB(OAc)3. After stirring for 24 hours at RT, the medium is diluted with DCM and then washed successively with water (twice), with saturated NaHCO3 solution and with saturated NaCl solution. The hydrochloride is prepared by adding Et2O saturated with HCl gas to a solution of the compound in EtOAc/Et2O (1/1; v/v). 0.36 g of the expected compound is obtained, which crystallizes from an EtOAc/Et2O mixture; m.p.=166° C.

αD25=−7.8 (c=1; MeOH).

[0619] NMR: 1.4-2.05 ppm: unres.: 13H; 2.2-4.8 ppm: unres.: 16H; 6.15 ppm: s: 2H; 6.65-7.8 ppm: unres.: 9H; 8.5 ppm: d: 1H; 9.95 ppm: bs: 1H.

EXAMPLE 38

[0620]

398

A) (R,R)-2-((3-(N-Boc)amino-3-(benzo[1,3]dioxol-5-yl)propanoyl)amino)-3-(4-(diisopropylaminomethyl)phenyl)-N-isopropyl-N-methylpropionamide

[0621] 237 mg of the compound obtained in Preparation 1.13 and the compound obtained in Preparation 2.19 are mixed with 303 mg of BOP and 360 μl of DIPEA in 10 ml of DCM. After stirring for 3 hours at RT, the mixture is concentrated to dryness. The residue is extracted with EtOAc and then washed successively with water, with saturated NaHCO3 solution and with saturated NaCl solution. 420 mg of the expected compound are obtained.

B) (R,R)-2-((3-Amino-3-(benzo[1,3]dioxol-5-yl)propanoyl)amino)-3-(4-(diisopropylaminomethyl)phenyl)-N-isopropyl-N-methylpropionamide, 2TFA

[0622] 420 mg of the compound from the preceding step are placed in 15 ml of TFA and 20 ml of DCM. After stirring for 2 hours at RT, the mixture is concentrated to dryness. 0.504 g of the expected compound is obtained, which crystallizes from Et2O.

C) (R,R)-2-((3-Naphthalene-2-sulphonylamino-3-(benzo[1,3]dioxol-5-yl)propanoyl)amino)-3-(4-(diisopropylaminomethyl)phenyl)-N-isopropyl-N-methylpropionamide

[0623] 500 mg of the compound from the preceding step are mixed with 153 mg of 2-naphthalenesulphonyl chloride and 354 μl of DIPEA in 5 ml of DCM, and the mixture is stirred for 2 hours at RT. The reaction mixture is concentrated to dryness and then washed with water, followed by saturated NaHCO3 solution. The hydrochloride is prepared by addition of Et2O saturated with HCl to a solution of the compound of EtOAc. 0.280 g of the expected compound is obtained.

[0624] NMR: 0.6-1.6 ppm: unres.: 18H; 2.2-3.1 ppm: unres.: 7H; 3.4-5.8 ppm: unres.: 9H; 6.3-8.6 ppm: unres.: 16H; 9.1 ppm: bs: 1H. αD25=+55.2° (c=1; MeOH).

[0625] By working as in the above examples, the compounds according to the invention of formula (I) described in Table IX are prepared.

9TABLE IX(I)

399

ExampleR1R2R3R4R539 2HCl

400

401

402

403

40 HCl

404

405

406

407

41 HCl

408

409

410

411

42 HCl

412

413

414

415

43 2HCl

416

417

418

419

44 HCl

420

421

422

423

424

425

426

427

428

429

430

431

47 HCl

432

433

434

435

436

437

438

439

440

441

442

443

50 HCl

444

445

446

447

51 HCl

448

449

450

451

52 HCl

452

453

454

455

456

457

458

459

54 HCl

460

461

462

463

55 HCl

464

465

466

467

56 HCl

468

469

470

471

472

473

474

475

476

477

478

479

480

481

482

483

60 2HCl

484

485

486

487

61 HCl

488

489

490

491

62 HCl

492

493

494

495

496

497

498

499

500

501

502

503

504

505

506

507

508

509

510

511

512

513

514

515

68 HCl

516

517

518

519

69 HCl

520

521

522

523

524

525

526

527

71 2HCl

528

529

530

531

72 HCl

532

533

534

535

536

537

538

539

540

541

542

543

75 HCl

544

545

546

547

548

549

550

551

77 HCl

552

553

554

555

78 2HCl

556

557

558

559

79 2HCl

560

561

562

563

80 2HCl

564

565

566

567

81 HCl

568

569

570

571

82 2HCl

572

573

574

575

83 HCl

576

577

578

579

84 2HCl

580

581

582

583

584

585

586

587

86 HCl

588

589

590

591

87 HCl

592

593

594

595

88 HCl

596

597

598

599

600

601

602

603

90 2HCl

604

605

606

607

91 HCl

608

609

610

611

612

613

614

615

616

617

618

619

620

621

622

623

624

625

626

627

96 HCl

628

629

630

631

97 HCl

632

633

634

635

98 HCl

636

637

638

639

99 HCl

640

641

642

643

100 HCl

644

645

646

647

101 HCl

648

649

650

651

102 HCl R isomer

652

653

654

655

103 HCl

656

657

658

659

104 HCl R, R isomer

660

661

662

663

105 HCl R isomer

664

665

666

667

106

668

669

670

671

107

672

673

674

675

108 HCl R, R Isomer

676

677

678

679

109 HCl R, R Isomer

680

681

682

683

EXAMPLE 39

[0626] NMR: 1.3-1.8 ppm: unres.: 4H; 2.2-3.6 ppm: unres.: 21H; 4.2-4.8 ppm: unres.: 2H; 6.8-8.4 ppm: unres.: 18H; 10.2 ppm: bs: 1H.

EXAMPLE 41

[0627] NMR: 1.1-1.8 ppm: unres.: 10H; 2.4-3.4 ppm: unres.: 12H; 4.2 ppm: bs: 2H; 4.4-4.8 ppm: unres.: 2H; 6.5-8.5 ppm: unres.: 18H; 10.3 ppm: bs: 1H.

EXAMPLE 42

[0628] NMR: 1.2-2 ppm: unres.: 10H; 2.4-3.3 ppm: unres.: 12H; 4.2-4.9 ppm: unres.: 4H; 6.9-9.1 ppm: unres.: 14H; 10.2 ppm: bs: 1H.

EXAMPLE 43

[0629] NMR: 1-1.8 ppm: unres.: 10H; 2.2-3.5 ppm: unres.: 18H; 4-5 ppm: unres.: 4H; 6.6-8.7 ppm: unres.: 17H; 10.7 ppm: bs: 1H.

EXAMPLE 44

[0630] NMR; 1.2 ppm: mt: 6H; 1.4-1.8 ppm: unres.: 4H; 2.2-3.3 ppm: unres.: 15H; 4.1-4.9 ppm: wires.: 4H; 6.8-8.1 ppm: wires.: 12H; 8.35 ppm: d: 1H; 8.9 ppm: d: 1H; 10.25 ppm: bs: 1H.

EXAMPLE 47

[0631] NMR: 0.5-1.3 ppm: unres.: 12H; 2.1-3.1 ppm: unres.: 11H; 3.6-5 ppm: unres.: 5H; 6.2-8.5 ppm: unres.: 22H; 10.1 ppm: bs: 1H.

EXAMPLE 48

[0632] NMR: 1 ppm: s: 9H; 1.2-1.7 ppm: unres.: 4H; 1.9 ppm: s: 3H; 2.2-3.5 ppm: unres.: 10H; 4.1-4.75 ppm: unres.: 2H; 6.7-8.4 ppm: unres.: 18H.

EXAMPLE 49

NMR: 0.95 ppm: t: 6H; 2.1-3.6 ppm: unres.: 10H; 4.4-5.1 ppm: unres.: 2H; 6.6-8.6 ppm: unres.: 23H.

EXAMPLE 50

[0633] NMR: 1.3-1 ppm: unres.: 6H; 1.7-3.5 ppm: unres.: 16H; 4-5 ppm: unres.: 5H; 5.2-5.7 ppm: unres.: 2H; 6.2-8.4 ppm: unres.: 21H; 10.7 ppm: bs: 1H.

EXAMPLE 51

[0634] NMR: 0.4-1.3 ppm: unres.: 12H; 2.1-3.1 ppm: unres.: 11H; 3.6-4.8 ppm: unres.: 5H; 6.6-8.5 ppm: unres.: 17H; 10 ppm: bs: 1H.

EXAMPLE 52

[0635] NMR: 1.15 ppm: t: 6H; 2.2 ppm: t: 2H; 2.5 ppm: s: 3H; 2.7-3.1 ppm: unres.: 6H; 3.8 ppm: t: 2H; 4.15 ppm: d: 2H; 4.9 ppm: q: 1H; 6.9-7.7 ppm: unres.: 16H; 8.5 ppm: d: 1H; 10.5 ppm: bs: 1H.

EXAMPLE 53

[0636] NMR: 0.4-1.5 ppm: unres.: 20H; 2-2.85 ppm: unres.: 11H; 3.3-4.8 ppm: unres.: 5H; 5.2-5.7 ppm: 2s: 2H; 6.2-8.3 ppm: unres.: 16H.

EXAMPLE 54

[0637] NMR: 1.4 ppm: s: 9H; 2-2.6 ppm: unres.: 8H; 2.9 ppm: d: 2H; 3.6-4.6 ppm: unres.: 4H; 4.9 ppm: q: 1H; 6.9-7.7 ppm: unres.: 16H; 8.5 ppm: d: 1H; 9.9 ppm: bs: 1H.

EXAMPLE 55

[0638] NMR: 1.15 ppm: t: 6H; 2.4 ppm: s: 3H; 2.8-3.1 ppm: unres.: 6H; 3.75 ppm: t: 2H; 4.15 ppm: d: 2H; 4.9 ppm: q: 1H; 5.95 ppm: s: 2H; 6.4-7.7 ppm: unres.: 14 H; 8.4 ppm: d: 1H; 10.1 ppm: bs: 1H.

EXAMPLE 56

[0639] NMR: 0.7-1.3 ppm: unres.: 12H; 2.2 ppm: t: 2H; 2.3-3.1 ppm: unres.: 12H; 3.6-5 ppm: unres.: 6H; 6 ppm: s: 2H; 6.4-7.7 ppm: unres.: 9H; 8.3 ppm: dd: 1H; 10.2 ppm: bs: 1H.

EXAMPLE 57

[0640] NMR: 1.05 ppm: ds: 9H; 1.85 ppm: ds: 3H; 2.1-3.4 ppm: unres.: 6H; 4.3-4.9 ppm: unres.: 2H; 5.2-5.8 ppm: 2ds: 2H; 6.1-8.4 ppm: unres.: 21H.

EXAMPLE 60

[0641] NMR: 1.25 ppm: t: 6H; 2.3 ppm: mt: 2H; 2.5 ppm: s: 3H; 3 ppm: mt: 6H; 3.85 ppm: t: 2H; 4.25 ppm: d: 2H; 5.25 ppm: q: 1H; 6.1 ppm: s: 2H; 6.4-9.2 ppm: unres.: 14H; 10.7 ppm: bs: 1H.

EXAMPLE 61

[0642] NMR: 1.2 ppm: t: 6H; 2.3 ppm: mt: 2H; 2.5 ppm: s: 3H; 2.7-3.3 ppm: unres.: 6H; 3.9 ppm: t: 2H; 4.2 ppm: d: 2H; 5.1 ppm: q: 1H; 6.1-7.8 ppm: unres.: 14H; 8.45 ppm: d: 1H; 10.6 ppm: bs: 1H.

EXAMPLE 62

[0643] NMR: 0.5-1.4 ppm: unres.: 12H; 2-5 ppm: unres.: 16H; 5.95-8.5 ppm: unres.: 16H.

EXAMPLE 64

[0644] NMR: 0.6-1.3 ppm: unres.: 15H; 2.2-3.25 ppm: unres.: 9H; 3.4-5 ppm: unres.: 8H; 5.3-5.8 ppm: unres.: 2H; 6.3-8.5 ppm: unres.: 16H.

EXAMPLE 65

[0645] NMR: 0.85 ppm: td: 6H; 2.1-2.9 ppm: unres.: 8H; 3.2-4.7 ppm: unres.: 6H; 5.2-5.8 ppm: unres.: 3H; 6.1-8.3 ppm: unres.: 19H.

EXAMPLE 68

[0646] NMR: 0.6-1.3 ppm: unres.: 12H; 2-3.1 ppm: unres.: 17H; 3.6-5 ppm: unres.: 6H; 6.7-7.7 ppm: unres.: 10H; 8.3 ppm: dd: 1H; 9.9 ppm: bs: 1H.

EXAMPLE 69

[0647] NMR: 0.65-1.3 ppm: unres.: 12H; 2.15 ppm: t: 2H; 2.25 ppm: s: 3H; 2.3-3.1 ppm: unres.: 9H; 3.7-5 ppm: unres.: 6H; 7-7.7 ppm: unres.: 11H; 8.4 ppm: dd: 1H; 9.9 ppm: bs: 1H.

EXAMPLE 71

[0648] NMR: 1.2 ppm: t: 6H; 2.1-2.6 ppm: unres.: 5H; 2.8-3.5 ppm: unres.: 6H; 3.9 ppm: t: 2H; 4.2 ppm: bd: 2H; 5.2 ppm: q: 1H; 7-7.8 ppm: unres.: 13H; 8.9 ppm: d: 1H; 10.4 ppm: bs: 1H; 14.4 ppm: bs: 2H.

EXAMPLE 72

[0649] NMR: 1-1.9 ppm: unres.: 10H; 2.2 ppm: t: 2H; 2.5 ppm: s: 3H; 2.6-3.5 ppm: unres.: 10H; 3.75 ppm: t: 2H; 4.15 ppm: d: 2H; 4.5 ppm: q: 1H; 6 ppm: s: 2H; 6.3-7.7 ppm: unres.: 9H; 8.3 ppm: d: 1H; 10.2 ppm: s: 1H.

EXAMPLE 73

[0650] NMR: 0.9 ppm: mt: 6H; 1.3 ppm: mt: 2H; 1.9-2.2 ppm: unres.: 5H; 2.25-3.6 ppm: unres.: 12H; 4.2 ppm: mt: 1H; 4.55 ppm: mt: 1H; 5.2-5.7 ppm: unres.: 2H; 6.2-8.4 ppm: unres.: 17H.

EXAMPLE 74

[0651] NMR: 0.9 ppm: mt: 6H; 1.3-3.5 ppm: unres.: 19H; 4.3-4.8 ppm: unres.: 2H; 5.2-5.7 ppm: unres.: 2H; 6.2-8.4 ppm: unres.: 16H.

EXAMPLE 77

[0652] NMR: 0.5-1.4 ppm: unres.: 18H; 2.1-3 ppm: unres.: 8H; 3.3-4.8 ppm: unres.: 7H; 5.2-5.65 ppm: unres.: 2H; 6.2-8.4 ppm: unres.: 16H; 9.15 ppm: s: 1H.

EXAMPLE 78

[0653] NMR: 1.4 ppm: s: 9H; 2.2-2.75 ppm: unres.: 5H; 2.9 ppm: t: 2H; 3.8 ppm: mt: 1H; 4.5 ppm: mt: 2H; 5 ppm: mt: 1H; 5.1-5.7 ppm: 2ds: 2H; 6.2-9.1 ppm: unres.: 20H; 9.6 ppm: s: 1H.

EXAMPLE 79

[0654] NMR: 0.7-1.3 ppm: unres.: 12H; 2.1-3.1 ppm: unres.: 11H; 3.7-5 ppm: unres.: 6H; 7.1-8.2 ppm: unres.: 16H; 10.85 ppm: s: 1H.

EXAMPLE 80

[0655] NMR: 0.7-1.4 ppm: unres.: 12H; 2.2-3.2 ppm: unres.: 14H; 3.7-5 ppm: unres.: 6H; 7-8.8 ppm: unres.: 11H; 10.8 ppm: s: 1H.

EXAMPLE 82

[0656] NMR: 0.7-1.5 ppm: unres.: 12H; 2.4-3.3 ppm: unres.: 14H; 4-5.1 ppm: unres.: 6H; 7.2-9 ppm: unres.: 11H; 10.8 ppm: s: 1H.

EXAMPLE 83

[0657] NMR: 1.4 ppm: s: 9H; 2-3 ppm: unres.: 10H; 3.6-4.6 ppm: unres.: 4H; 4.9 ppm: mt: 1H; 5.95 ppm: s: 2H; 6.3-7.7 ppm: unres.: 14H; 8.4 ppm: d: 1H; 9.65 ppm: bs: 1H.

EXAMPLE 84

[0658] NMR: 0.6-1.4 ppm: unres.: 12H; 2.4-3.2 ppm: unres.: 11H; 3.8-5.1 ppm: unres.: 6H; 7-8.9 ppm: unres.: 15H; 10.9 ppm: bs: 2H.

EXAMPLE 85

[0659] NMR: 1 ppm: t: 6H; 2.3-3.7 ppm: unres.: 10H; 4.7 ppm: mt: 1H; 5 ppm: mt: 1H; 5.1-5.8 ppm: 2ds: 2H; 6.2-8.8 ppm: unres.: 20H; 12.15 ppm: sd: 1H.

EXAMPLE 86

[0660] NMR: 1.2-2.55 ppm: unres.: 16 H; 2.85 ppm: mt: 2H; 3.1-4.4 ppm: unres.: 5H; 4.9 ppm: mt: 1H; 6.8-7.6 ppm: unres.: 16H; 8.4 ppm: d: 1H; 10.6 ppm: bs: 1H.

EXAMPLE 87

[0661] NMR: 0.6-1.3 ppm: unres.: 12H; 2.2 ppm: t: 2H; 2.35-3.1 ppm: unres.: 12H; 3.7-5 ppm: unres.: 6H; 6.9-7.8 ppm: unres.: 9H; 8.3 ppm: dd: 1H; 10.1 ppm: bs: 1H.

EXAMPLE 88

[0662] NMR: 0.7-1.3 ppm: unres.: 12H; 2.3 ppm: t: 2H; 2.4-3.1 ppm: unres.: 12H; 3.5-5 ppm: unres.: 6H; 7-7.7 ppm: unres.: 10H; 8.4 ppm: dd: 1H; 10 ppm: bs: 1H.

EXAMPLE 89

[0663] NMR: 1.05 ppm: s: 9H; 1.85 ppm: s: 3H; 2.2 ppm: t: 2H; 2.6 ppm: s: 3H; 2.8 ppm: mt: 2H; 3.4 ppm: s: 2H; 3.7 ppm: s: 2H; 4.9 ppm: mt: 1H; 5.95 ppm: s: 2H; 6.3-8.6 ppm: unres.: 14H.

EXAMPLE 90

[0664] NMR: 1.5 ppm: s: 9H; 2.2-2.6 ppm: unres.: 8H; 3 ppm: unres.: 2H; 3.8-4.6 ppm: 2 unres.: 4H; 5.2 ppm: unres.: 1H; 7-9.2 ppm: unres.: 17H; 10 ppm: bs: 1H.

EXAMPLE 91

[0665] NMR: 1.5 ppm: s: 9H; 2.3 ppm: t: 2H; 2.4-2.6 ppm: unres.: 6H; 3 ppm: bd: 2H; 3.8-4.1 ppm: unres.: 3H; 4.4-5 ppm: unres.: 2H; 6 ppm: s: 2H; 6.6-7.8 ppm: unres.: 14H; 8.5 ppm: d: 1H; 10.4 ppm: bs: 1H.

EXAMPLE 94

[0666] NMR: 0.9 ppm: t: 6H; 2.1-3.7 ppm: unres.: 14H; 4.50 ppm: mt: 1H; 5-5.7 ppm: unres.: 3H; 6-8.6 ppm: unres.: 20H.

EXAMPLE 95

[0667] NMR: 0.95 ppm: t: 6H; 2.1-2.7 ppm: unres.: 9H; 3-4 ppm: unres.: 9H; 5.4 ppm: q: 1H; 6.05 ppm: s: 2H; 6.4-7.8 ppm: unres.: 13H; 8.7 ppm: d: 1H.

EXAMPLE 99

[0668] NMR: 1.5 ppm: s: 9H; 1.9-2.55 ppm: unres.: 10H; 2.6-3.1 ppm: unres.: 6H: 3.75-4.8 ppm: unres.: 4H; 5.05 ppm: mt: 1H; 6.7-7.8 ppm: unres.: 14H; 8.55 ppm: d: 1H; 9.4 ppm: bs: 1H.

EXAMPLE 101

[0669] NMR: 0.5-1.4 ppm: unres.: 18H; 2.1-3 ppm: unres.: 7H; 3.3-4.9 ppm: unres.: 11H; 6.1-8.4 ppm: unres.: 16H; 9.1 ppm: bs: 1H.

EXAMPLE 102

[0670] NMR: 0.8-1.2 ppm: unres.: 6H; 1.5 ppm: s: 9H; 2.2-3.1 ppm: unres.: 13H; 3.7-5.1 ppm: unres.: 6H: 6.1 ppm: s: 1H; 6.5-7.8 ppm: unres.: 9H; 8.3-8.6 ppm: unres.: 1H; 9.5 ppm: bs: 1H.

EXAMPLE 103

[0671] NMR: 0.8-1.5 ppm: unres.: 12H; 2.35 ppm: t: 2H; 2.5 ppm: s: 6H; 2.6-3.1 ppm: unres.: 5H; 3.45 ppm: mt: 1H; 3.8-5.1 ppm: unres.: 6H; 6.15 ppm: s: 2H; 6.5-7.8 ppm: unres.: 9H; 8.3-8.6 ppm: unres.: 1H; 10.5 ppm: bs: 1H.

EXAMPLE 104

[0672] NMR: 0.7 ppm: mt: 6H; 1.4 ppm: s: 9H; 2.2-3 ppm: unres.: 10H; 3.8 ppm: mt: 1H; 4.3-4.9 ppm: unres.: 4H; 5.2-5.7 ppm: 2s: 2H; 6.2-8.4 ppm: unres.: 16H; 9.5 ppm: s: 1H.

EXAMPLE 105

[0673] NMR: 1.4-2.4 ppm: unres.: 14H; 2.5 ppm: s: 6H; 2.65 to 5 ppm: unres.: 12H; 6.1 ppm: s: 2H; 6.5-7.8 ppm: unres.: 9H; 8.45 ppm: d: 1H; 10.8 ppm: bs: 1H.

EXAMPLE 106

[0674] MH+: 765

EXAMPLE 107

[0675] MH+: 765

EXAMPLE 108

[0676] NMR: 0.6 to 1.4 ppm: unres.: 15H; 2.1 to 3.5 ppm: unres.: 11H; 3.6 to 4.9 ppm: unres.: 5H; 5.2 to 5.7 ppm: 2s: 2H; 6.2 to 6.5 ppm: unres.: 3H; 7 to 8.4 ppm: unres.: 13H; 9.8 ppm: bs: 1H.

EXAMPLE 109

[0677] NMR: 0.5 to 1.5 ppm: unres.: 18H; 2.1 to 3 ppm: unres.: 10H; 3.4 to 4.9 ppm: unres.: 7H; 5.75 to 5.80 ppm: 2s: 2H; 6.2 to 6.65 ppm: unres.: 3H; 7 to 7.6 ppm: unres.: 7H; 8.1 to 8.4 ppm: unres.: 2H; 8.8 ppm: bs: 1H.

EXAMPLE 110

[0678]

684

[0679] A mixture containing 250 mg of the compound from Preparation 3.8, 74 mg of 2-naphthalenesulphonyl chloride and 171 μl of DIPEA in 10 ml of DCM is stirred overnight at RT. The medium is concentrated to dryness and is then taken up in EtOAc and washed with water and with saturated NaHCO3 solution. The resulting solution is extracted with a pH 2 buffer and then basified to pH 8 with saturated NaHCO3 solution. This mixture is extracted with DCM and then washed with saturated NaCl solution. The resulting mixture is taken up in an EtOAc/Et2O mixture and the hydrochloride is prepared by addition of hydrochloric ether. 140 mg of the expected compound are obtained.

[0680] NMR: 0.7 to 2.1 ppm: unres.: 18H; 2.3 to 3.2 ppm: unres.: 9H; 3.3 ppm: mt: 1H; 3.8 to 5 ppm: unres.: 4H; 5.4 to 5.8 ppm: 2s: 2H; 6.4 to 6.8 ppm: unres.: 3H; 7.1 to 8.2 ppm: unres.: 11H; 8.2 to 8.5 ppm: mt: 2H; 9.4 to 10.3 ppm: 2 bs: 1H.

αD25=+50° (c=1; MeOH)

[0681] MH+: 727

EXAMPLE 111

[0682]

685

[0683] This compound is prepared by working according to the example described above, starting with the compound from Preparation 3.8 and 6-methoxynaphthalene-2-sulphonyl chloride obtained according to J. Med. Chem., 1999, 42, 3557-3571.

[0684] NMR: 0.6 to 2 ppm: unres.: 18H; 2.2 to 3.6 ppm: unres.: 10H; 3.8 to 5 ppm: unres.: 7H; 5.4 to 5.8 ppm: 2s: 2H; 6.3 to 6.6 ppm: unres.: 3H; 7.1 to 8.1 ppm: unres.: 10H; 8.1 to 8.5 ppm: unres.: 2H; 9.4 to 10.2 ppm: 2bs: 1H.

αD25=+32° (c=1; MeOH)

[0685] MH+: 757

EXAMPLE 111a

[0686]

686

[0687] 152 mg of the compound of Example 111 are placed in a mixture of 50 ml of DCM and 20 ml of saturated NaHCO3 solution. The organic phase obtained is dried and then concentrated to give the compound in the form of a base. The medium is taken up in 5 ml of DCM and treated with 75 mg of 60% meta-chloroperbenzoic acid for 1 hour at RT. The reaction mixture is extracted with DCM and the organic phase is then washed with saturated NaCl solution. 0.14 g of the expected compound is obtained, which crystallizes from methyl tert-butyl ether.

[0688] MH+: 773 NMR: 0.6 to 1.8 ppm: unres.: 18H; 2.2 to 3.4 ppm: unres.: 10H; 3.8 ppm: s: 3H; 4.3 to 4.9 ppm: unres.: 4H ; 5.4 to 5.6 ppm: d: 2H; 6.3 ppm: mt: 3H; 7.1 to 7.8 ppm: unres.: 10H.

EXAMPLE 112

[0689]

687

[0690] 225 mg of the product obtained in Preparation 3.9 are placed in 3 ml of TFA and 10 ml of DCM, and the mixture is stirred for 30 minutes at RT. The medium is concentrated and then triturated in iPr2O. The oil formed is decanted off. The oil formed is taken up in 5 ml of DCM and treated with 100 μl of TEA and then 82 mg of 2-naphthalenesulphonyl chloride, while maintaining the pH at 7 by adding TEA. The medium is stirred overnight at RT and is then concentrated. It is extracted with EtOAc and washed with water (3 times) and with saturated NaCl solution. The hydrochloride is prepared, which crystallizes from an EtOAc/Et2O mixture.

[0691] NMR: 0.5 to 2 ppm: unres.: 16H; 2.2 to 3 ppm: unres.: 11H; 3.2 to 4.8 ppm: unres.: 5H; 5.3 and 5.6 ppm: 2s: 2H; 6.3 to 6.6 ppm: unres.: 3H; 7 to 7.3 ppm: unres.: 2H; 7.4 to 7.8 ppm: unres.: 6H; 7.9 to 8.1 ppm: unres.: 5H; 8.3 ppm: mt: 1H. αD25=+42.1° (c=1; MeOH) MH+: 727

EXAMPLE 113

[0692]

688

[0693] This compound is prepared according to the examples described above, starting with the compound from Preparation 3.8 and the compound from Preparation 1.33, step A.

[0694] MH+: 777

EXAMPLE 114

[0695]

689

[0696] This compound is prepared according to the examples described above, starting with the compound from Preparation 3.10 and the sulphonyl chloride described in Preparation 1.33, step A.

[0697] MH+: 785

[0698] Other compounds of formula (I) according to the invention were prepared by using the methods described above and identified by their mass spectrum and their NMR spectrum. They are described in the following 2 Tables:

10TABLE X(I)

690

ExamplesR1R3R4

691

Salt115

692

693

694

695

—116 (R, R)

696

697

698

699

HCl117

700

701

702

703

HCl118

704

705

706

707

HCl119 (R, R)

708

709

710

711

HCl120

712

713

714

715

HCl121

716

717

718

719

HCl122 (R, R)

720

721

722

723

HCl123 (R, R)

724

725

726

727

HCl124 (R, R)

728

729

730

731

HCl125 (R, R)

732

733

734

735

HCl126

736

737

738

739

—127

740

741

742

743

HCl128

744

745

746

747

—129 —

748

749

750

751

HCl130 —

752

753

754

755

HCl131 —

756

757

758

759

HCl132 —

760

761

762

763

HCl133

764

765

766

767

HCl134 (R, R)

768

769

770

771

HCl135 (R, R)

772

773

774

775

HCl136 —

776

777

778

779

HCl137 —

780

781

782

783

HCl138 —

784

785

786

787

HCl139 —

788

789

790

791

HCl140 —

792

793

794

795

HCl141 (R, R)

796

797

798

799

HCl142 (R, R)

800

801

802

803

HCl143 —

804

805

806

807

HCl144 (R, R)

808

809

810

811

HCl145 —

812

813

814

815

HCl146 —

816

817

818

819

HCl147 —

820

821

822

823

HCl148 (R, R)

824

825

826

827

HCl149

828

829

830

831

—150

832

833

834

835

HCl151

836

837

838

839

HCl152

840

841

842

843

HCl153

844

845

846

847

HCl154

848

849

850

851

HCl155

852

853

854

855

HCl156

856

857

858

859

157

860

861

862

863

HCl158

864

865

866

867

HCl159

868

869

870

871

HCl160

872

873

874

875

HCl161 (R, R)

876

877

878

879

HCl162 (R, R)

880

881

882

883

HCl163 (R, R)

884

885

886

887

HCl164 (R, R)

888

889

890

891

HCl165 (R, R) a)

892

893

894

895

HCl166 (R, R)

896

897

898

899

HCl167

900

901

902

903

HCl168

904

905

906

907

HCl169 (R, R)

908

909

910

911

HCl170 (R, R)

912

913

914

915

HCl171 (R, R) a)

916

917

918

919

HCl172 (R, R)

920

921

922

923

HCl173 (R, R)

924

925

926

927

HCl174 (R, R)

928

929

930

931

HCl175

932

933

934

935

HCl176 (R, R)

936

937

938

939

HCl177 —

940

941

942

943

HCl178 (R, R)

944

945

946

947

HCl179 R, (R, S)

948

949

950

951

HCl180

952

953

954

955

HCl181 (R, R)

956

957

958

959

HCl182

960

961

962

963

HCl183

964

965

966

967

HCl184 (R, R)

968

969

970

971

HCl185 (R, R)

972

973

974

975

HCl186 (R, R)

976

977

978

979

HCl187 (R, R)

980

981

982

983

HCl188 —

984

985

986

987

HCl189 (R, R)

988

989

990

991

HCl190 —

992

993

994

995

HCl191 (R, (R, S))

996

997

998

999

HCl192 (R, R)

1000

1001

1002

1003

HCl193

1004

1005

1006

1007

HCl194 (R, R)

1008

1009

1010

1011

HCl195

1012

1013

1014

1015

HCl196 (R, R)

1016

1017

1018

1019

HCl197 —

1020

1021

1022

1023

HPF6198 (R, R)

1024

1025

1026

1027

HCl199

1028

1029

1030

1031

HCl200 (R, R)

1032

1033

1034

1035

HCl201 —

1036

1037

1038

1039

HCl202 (R, R)

1040

1041

1042

1043

HCl203

1044

1045

1046

1047

HCl204 (R, R)

1048

1049

1050

1051

HCl205 (R, R)

1052

1053

1054

1055

HCl206 —

1056

1057

1058

1059

HCl207 (R, R)

1060

1061

1062

1063

HCl208

1064

1065

1066

1067

HCl209 (R, R)

1068

1069

1070

1071

HCl210 (R, R)

1072

1073

1074

1075

—211

1076

1077

1078

1079

HCl212 —

1080

1081

1082

1083

HCl213 —

1084

1085

1086

1087

HCl214 (R, R)

1088

1089

1090

1091

HCl215 (R, R)

1092

1093

1094

1095

HCl216 (R, R)

1096

1097

1098

1099

HCl217 (R, R)

1100

1101

1102

1103

HCl218 (R, R)

1104

1105

1106

1107

HCl219

1108

1109

1110

1111

HCl220 —

1112

1113

1114

1115

HCl221 —

1116

1117

1118

1119

HCl222 (R, R)

1120

1121

1122

1123

HCl223 (R, R)

1124

1125

1126

1127

HCl224 (R, R)

1128

1129

1130

1131

HCl225 (R, R)

1132

1133

1134

1135

HCl226 (R, R)

1136

1137

1138

1139

HCl227 (R, R)

1140

1141

1142

1143

HCl228

1144

1145

1146

1147

HCl229 (R, R)

1148

1149

1150

1151

HCl230 (R, R)

1152

1153

1154

1155

HCl231 (R, R)

1156

1157

1158

1159

HCl232 (R, R)

1160

1161

1162

1163

HCl233 (R, R)

1164

1165

1166

1167

HCl234 —

1168

1169

1170

1171

HCl235 —

1172

1173

1174

1175

HCl236 (R, R)

1176

1177

1178

1179

HCl237 ((R, S), R)

1180

1181

1182

1183

HCl238 ((R, S), R)

1184

1185

1186

1187

HCl239 (R, R)

1188

1189

1190

1191

HCl240 (R, R)

1192

1193

1194

1195

HCl241 (R, R)

1196

1197

1198

1199

HCl242

1200

1201

1202

1203

HCl243

1204

1205

1206

1207

HCl244

1208

1209

1210

1211

HCl245 (R, R)

1212

1213

1214

1215

HCl246 (R, R)

1216

1217

1218

1219

HCl247 (R, R)

1220

1221

1222

1223

HCl248 (R, R)

1224

1225

1226

1227

HCl249 ((R), R, S))

1228

1229

1230

1231

HCl250 (R, R)

1232

1233

1234

1235

HCl251 (R, R)

1236

1237

1238

1239

HCl252 (R, R)

1240

1241

1242

1243

HCl253 (R, R)

1244

1245

1246

1247

HCl254 (R, R)

1248

1249

1250

1251

HCl255 (R, R)

1252

1253

1254

1255

HCl256 (R, R)

1256

1257

1258

1259

HCl257

1260

1261

1262

1263

HCl258 ((R, S), R)

1264

1265

1266

1267

HCl259 (R, R)

1268

1269

1270

1271

HCl260 (R, R)

1272

1273

1274

1275

HCl261 (R, R)

1276

1277

1278

1279

HCl262 ((R, S), R)

1280

1281

1282

1283

HCl263 (R, R)

1284

1285

1286

1287

HCl264

1288

1289

1290

1291

HCl265

1292

1293

1294

1295

HCl266 (R, R)

1296

1297

1298

1299

HCl267 (R, R)

1300

1301

1302

1303

HCl268 (R, R)

1304

1305

1306

1307

HCl269 (R, R)

1308

1309

1310

1311

HCl270 (R, R)

1312

1313

1314

1315

HCl271 (R, R)

1316

1317

1318

1319

HCl272 ((R, S), R)

1320

1321

1322

1323

HCl273 (R, R)

1324

1325

1326

1327

HCl274 (R, R)

1328

1329

1330

1331

HCl275 (R, R)

1332

1333

1334

1335

HCl276 (R, R)

1336

1337

1338

1339

HCl277

1340

1341

1342

1343

HCl278 (R, R)

1344

1345

1346

1347

HCl279 (R, R)

1348

1349

1350

1351

HCl280 (R, R)

1352

1353

1354

1355

HCl281 (R, R)

1356

1357

1358

1359

HCl282 ((R, S), R)

1360

1361

1362

1363

HCl283 ((R, S), R)

1364

1365

1366

1367

HCl284 (R, R)

1368

1369

1370

1371

HCl285 (R, R)

1372

1373

1374

1375

HCl286

1376

1377

1378

1379

HCl287

1380

1381

1382

1383

HCl288 (R, R)

1384

1385

1386

1387

HCl289 ((R, S), R)

1388

1389

1390

1391

HCl290 (R, R)

1392

1393

1394

1395

HCl291 ((R, S), R)

1396

1397

1398

1399

HCl292 (R, R)

1400

1401

1402

1403

HCl293 ((R, S), R)

1404

1405

1406

1407

HCl294 (R, R)

1408

1409

1410

1411

HCl295 ((R, S), R)

1412

1413

1414

1415

HCl296 (R, R)

1416

1417

1418

1419

HCl297 (R, R)

1420

1421

1422

1423

HCl298 ((R, S), R)

1424

1425

1426

1427

HCl299 (R, R)

1428

1429

1430

1431

HCl300 (R, R)

1432

1433

1434

1435

HCl301 (R, R)

1436

1437

1438

1439

HCl302 (R, R)

1440

1441

1442

1443

HCl303

1444

1445

1446

1447

HCl304 (S, R)

1448

1449

1450

1451

(CF3CO2H)305 242142A (R, R)

1452

1453

1454

1455

(CF3CO2H)306 (R, R)

1456

1457

1458

1459

HCl307 ((R, S), R)

1460

1461

1462

1463

HCl308 (S, R)

1464

1465

1466

1467

(CF3CO2H)309 (R, R)

1468

1469

1470

1471

(CF3CO2H)310 (R, R)

1472

1473

1474

1475

HCl311 ((R, S), R)

1476

1477

1478

1479

HCl312 (R, R)

1480

1481

1482

1483

HCl313 (S, S)

1484

1485

1486

1487

HCl314 (R, R)

1488

1489

1490

1491

HCl

[0699] a) For these compounds, the intermediate sulphonyl chloride (VI is obtained according to Preparation 1.32.

11TABLE XI(I)

1492

ExamplesR1R2R4R5Salt315 (R)

1493

1494

1495

1496

HCl316 (R)

1497

1498

1499

1500

HCl317

1501

1502

1503

1504

HCl318

1505

1506

1507

1508

HCl

[0700] Moreover, several compounds according to the invention were prepared by a “parallel synthesis” method by reacting various sulphonyl halides of formula R1SO2Hal with a compound of formula (V) in which Y represents CONR8R9 and Z represents CH2NR11R12.

EXAMPLES 319 to 335

[0701] A) A solution of 0.98 g of the compound from Preparation 3.2 is prepared in a mixture of 40 ml of CH3CN and 10 ml of DCM.

[0702] B) Tubes are prepared, each containing:

[0703] 1.014 ml of the solution prepared in A, i.e. 40 μmol;

[0704] 1.2 equivalents of sulphonyl chloride of formula R1SO2Cl, i.e. 48 μmol;

[0705] 1.5 equivalents of morpholinomethylpolystyrene resin at 3.64 mmol/g, i.e. 16.5 mg of resin.

[0706] C) The tubes are stirred for 24 hours at RT and are then each treated with 0.6 equivalent of tris(2-aminomethyl)amine resin, i.e. 6 mg.

[0707] Each tube is filtered and concentrated, and the medium is then taken up in DMSO to obtain a solution containing 1 μmol per litre of the expected compound. The nature of the compound formed and its purity are monitored by measuring the HPLC retention time (Rt in minutes) and the mass spectrum.

[0708] The solutions of the compounds according to the invention obtained are used without further modification to measure the affinity of the said compounds for the bradykinin B1 receptors.

[0709] In the examples below, the sulphonyl chlorides R1 SO2Cl used are commercially available.

12TABLE XII(I)

1509

ExamplesR1massRt (minutes)319

1510

7384.19320

1511

7044.02321

1512

7044.13322

1513

7384.2323

1514

7384.25324

1515

7384.19325

1516

6863.97326

1517

6963.56327

1518

6963.7328

1519

6783.72329

1520

6883.41330

1521

7104.17331

1522

7404.31332

1523

7204.2333

1524

7084.26334

1525

7724.33335

1526

7093.82

EXAMPLES 336 to 338

[0710] A) A solution of 1.924 g of the compound from Preparation 3.1 is prepared in 100 ml of CH3CN.

[0711] B) Tubes are prepared, each containing:

[0712] 941 μl of the solution prepared in A, i.e. 40 μmol;

[0713] 1.2 equivalents of sulphonyl chloride of formula R1SO2Cl, i.e. 48 μmol;

[0714] 1.5 equivalents of morpholinomethylpolystyrene resin at 3.64 mmol/g, i.e. 16.5 mg of resin;

[0715] 0.2 equivalent of DMAP/polystyrene resin, used as activator.

[0716] C) The tubes are stirred for 24 hours at RT and are then each treated with 0.6 equivalent of tris(2-aminoethyl)amine resin, i.e. 6 mg. Each tube is filtered and concentrated and then taken up in DMSO to obtain a solution containing 1 μmol per litre of the expected compound. The nature of the compound formed and its purity are monitored by measuring the HPLC retention time (Rt minutes) and the mass spectrum.

[0717] The solutions of the compounds according to the invention obtained are used in unmodified form to measure the affinity of the said compounds for the bradykinin B1 receptors.

13TABLE XIII(I)

1527

ExamplesR1massRt (minutes)336

1528

6604.49337

1529

6944.9338

1530

6965.1

EXAMPLES 339 to 353

Preparation

(R,R)-2-(((3-amino-3-benzo[1,3]dioxol-5-yl)propanoyl)amino)-3-(4-(diisopropylaminomethyl)phenyl)-N-isopropyl-N-methylpropionamide

[0718]

1531

[0719] A) 8.6 g of the trifluoroacetate obtained in Example 38, step B are dissolved in DCM and washed with a saturated NaHCO3 solution and then saturated NaCl. After drying and concentration, 5.43 g of the expected compound are obtained in the form of a dry foam.

[0720] B) A solution of 524.7 mg of the compound from Preparation A is prepared in 25 ml of CH3CN.

[0721] C) Tubes are prepared, each containing:

[0722] 1 ml of the solution prepared in B, i.e. 40 μmol;

[0723] 1.2 equivalents of sulphonyl chloride of formula R1SO2Cl, i.e. 48 μmol;

[0724] 1.5 equivalents of morpholinomethylpolystyrene resin at 3.64 μmol/g, i.e. 16.5 mg of resin:

[0725] D) The tubes are stirred overnight at RT and are then each treated with 0.6 equivalent of tris(2-aminomethyl)amine resin, i.e. 6 mg. Each tube is filtered and concentrated and then taken up in DMSO to obtain a solution containing 1 μmol per litre of the expected compound. The nature of the compound and its purity are monitored by measuring the HPLC retention time (Rt minutes) and the mass spectrum.

[0726] The solutions of the compounds according to the invention obtained are used without further modification to measure the affinity of the said compounds for the bradykinin B1 receptors.

14TABLE XIV(I)

1532

ExamplesR1MassRt minutes339

1533

7485.22340

1534

7044.79341

1535

7545.08342

1536

7404.78343

1537

7465.17344

1538

7065.06345

1539

7265.22346

1540

7324.94347

1541

7004.73348

1542

7164.9349

1543

6984.77350

1544

6984.8351

1545

6944.47352

1546

7324.96353

1547

6784.64

EXAMPLES 354 to 363

Preparation

(R,R)-2-(((3-Amino-3-benzo[1,3]dioxol-5-yl)propanoyl)amino)-3-(4-(N-methyl-N-cyclopentylaminomethyl)phenyl)-N-isopropyl-N,N-disopropylpropionamide

[0727]

1548

[0728] This compound is prepared from the compounds obtained in Preparations 2.47 and 1.13: these 2 compounds are reacted after the necessary deprotection to give, after deprotection, the expected compound in the form of a bis(trifluoroacetate).

[0729] A) 210 mg of this bis(trifluoroacetate) are dissolved in DCM and washed with saturated NaHCO3 solution and then with saturated NaCl. After drying and concentration, 141 mg of the expected compound are obtained in the form of an oil.

[0730] B) A solution of 141 mg of the compound from step A in 20 ml of CH3CN is prepared.

[0731] C) Tubes are prepared, each containing:

[0732] 1.48 ml of the solution prepared, i.e. 20 μmol;

[0733] 1.2 equivalents of sulphonyl chloride of formula R1SO2Cl, i.e. 24 μmol;

[0734] 1.5 equivalents of morpholinomethylpolystyrene resin at 3.64 μmol/g, i.e. 8.24 mg of resin.

[0735] D) The tubes are stirred overnight at RT and are then each treated with 0.6 equivalent of tris(2-aminomethyl)amine resin, i.e. 3 mg. Each tube is filtered, concentrated and then taken up in DMSO to give a solution containing 1 μmol per litre of the expected compound. The nature of the compound and its purity are monitored by measuring the HPLC retention time (Rt minutes) and the mass spectrum.

15TABLE XV(I)

1549

ExamplesR1MassRt minutes354

1550

7104.62355

1551

7444.87356

1552

7364.7357

1553

7364.83358

1554

7024.51359

1555

7324.73360

1556

7464.92361

1557

7504.79362

1558

7324.81363

1559

7504.95

Derivatives of n-(arylsulfonyl)beta-aminoacids comprising a substituted aminomethyl group, the preparation method thereof and the pharmaceutical compositions containing same

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