Patent Application
20120156700
This invention relates generally to methods of detecting urine gelsolin. In particular, the present invention relates to the detection of urine gelsolin-like polypeptides using specific binding agents.
The following description is provided to assist the understanding of the reader. None of the information provided or references cited is admitted to be prior art to the present invention.
Actin is the most abundant protein in animal cells and constitutes 10-20% of the protein of many nucleated cells and 30% of the protein of muscle cells. Actin molecules each bind an ATP molecule and self-assemble into long filaments during which the ATP is hydrolyzed into ADP.
Injury to animal tissues results in the release of actin into the extracellular space, including the bloodstream. Although approximately half of nonmuscle cell actin is F-actin, (the double-helical, rodlike, filament form of actin which is assembled from G-actin monomers), the ionic conditions of extracellular fluids favor actin polymerization, so that virtually all the actin released into the blood from dying cells would be expected to polymerize into filaments (Lind, S. E. et al., Am. Rev. Respir. Dis. 138:429-434 (1988)). In purified solutions, in the absence of filament-shortening proteins, actin filaments can easily attain lengths of several microns. Were some fraction of actin released from injured cells to be irreversibly denatured, however, or else bound to one of the intracellular actin-binding proteins discussed below, this actin would remain monomeric.
There are many proteins which naturally associate with actin (for a review of actin-binding proteins, see Stossel et al., Ann. Rev. Cell Biol. 1:353-402 (1985); Pollard et al., Ann. Rev. Biochem. 55:987-1035 (1986)). However, two proteins, gelsolin and DBP (vitamin D binding protein) are thought to be primarily responsible for binding extracellular actin. (Janmey et al., Blood 70:529-530 (1987).) Gelsolin is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is an 82-kDa protein with six homologous subdomains, referred to as S1-S6. Each subdomain is composed of a five-stranded β-sheet, flanked by two α-helices, one positioned perpendicular with respect to the strands and one positioned parallel. The N-terminus (S1-S3) forms an extended β-sheet, as does the C-terminal (S4-S6). (Kiselar et al. PNAS 100:3942-3947 (2003).) The protein is highly conserved and highly homologous among species. Gelsolin is located intracellularly (in cytosol and mitochondria) and extracellularly (in blood plasma). Koya et al., J Biol Chem 275 (20):15343-15349 (2000).
Gelsolin has several functions in regulating actin polymerization. First, gelsolin is involved in monomeric actin binding. In the presence of Ca2+, gelsolin binds two actin monomers. Gelsolin can also bind actin filaments by another actin binding site. Second, gelsolin binds two actin monomers to form a nucleus for actin polymerization and caps the barbed end of actin filaments. Thus, gelsolin is capable of both serving as a nucleus for actin polymerization and capping the ends of the nascent microfilaments. Finally, gelsolin has actin severing activity.
Because of the large amounts of actin in cells, the release of actin from dying cells provides sufficient actin to have a significant affect on the microenvironment, either by increasing the viscosity of extracellular fluids of plasma and/or by entrapping cells or by other, as yet unidentified toxic effects. Infusion of extracellular free actin is toxic to animal tissues, and especially to renal and cardiopulmonary systems. (Harper et al., Clin. Res. 36:625 A (1988); Haddad et al., PNAS 87: 1381-1385 (1990).) Acute renal failure is a complication of muscle injury and actin infusions in rats causes transient elevations of the blood urea nitrogen (BUN) and creatinine levels, consistent with renal failure. Free actin in the plasma may form filaments which may lead to multiple organ dysfunction syndrome. (Dahl et al., Shock 12(2):102-4 (1999).) Moreover, since each extracellular actin molecule in a filament has an ADP molecule associated with it, the presence of extracellular actin in the blood may tend to induce or augment platelet aggregation in a manner which may not be advantageous to the host. (Lind et al., Am. Rev. Respir. Dis. 138:429-434 (1988); Scarborough et al., Biochem. Biophy. Res. Commun. 100:1314-1319 (1981).) Consequently, plasma gelsolin has a vital function of scavenging actin released from dead and dying cells and plasma gelsolin levels appear to be an early prognostic marker in patients experiencing trauma. (Mounzer et al., Am. J. Respir. Crit. Care Med. 160:1673-81 (1999).)
This invention relates generally to the preparation of gelsolin binding agents and uses of the same. In particular, the present invention relates to a method for determining the presence of, or predisposition to, a disease or condition associated with altered levels of gelsolin in a mammalian subject, the method comprising the steps of: (a) contacting a urine sample from the mammalian subject under immunologically-reactive conditions with one or more antibodies or antibody-related polypeptides having the same antigen-binding specificity as antibodies produced by a deposited cell line selected from the group consisting of: CGMCC Accession Nos: 2114, 2116, 2247, and 2248; and (b) detecting the binding of the one or more antibodies or antibody-related polypeptides to the gelsolin-like polypeptide to determine the level of gelsolin-like polypeptide in the sample, wherein a difference between the level of gelsolin-like polypeptide in the sample and a reference level indicates a presence of, or predisposition to, a disease or condition associated with altered levels of gelsolin in the mammalian subject.
In one embodiment, the sample is contacted with the antibodies or antibody-related polypeptides in an ELISA. The step of contact may comprise binding a first antibody to a substrate and contacting the sample and a second antibody to the substrate, wherein the second antibody comprises a detectable label. In one embodiment, the first antibody binds to the same antigenic determinant as an antibody produced by a hybridoma cell line CGMCC Accession No: 2247 and the second antibody binds to the same antigenic determinant as an antibody produced by a hybridoma cell line selected from the group consisting of CGMCC Accession No. 2116.
The instant methods may be useful to diagnose a disease or condition associated with altered levels of gelsolin. The disease or condition associated with altered levels of gelsolin may be selected from the group consisting of: kidney failure, septic shock, multiple organ dysfunction syndrome, rheumatoid arthritis, stroke, heart infarction, cancer, systemic autoimmune disease, chronic hepatitis, side-effects of chemotherapy, and side-effects of radiation therapy.
In some embodiments, the gelsolin-like polypeptide being detected in urine gelsolin is a C-terminal fragment of gelsolin. For instance, an increase in the level of gelsolin-like polypeptide in the sample compared to the reference level indicates a presence of, or predisposition to, a disease or condition associated with altered levels of gelsolin in a mammalian subject.
In one embodiment, the reference level is the amount of the gelsolin-like polypeptide in a control population of subjects that do not have a disease or condition associated with altered levels of gelsolin. In another embodiment, the reference level is the amount of the gelsolin-like polypeptide in a subject that does not have a disease or condition associated with altered levels of gelsolin. In yet another embodiment, the reference level is the amount of the gelsolin-like polypeptide in the subject at an earlier time.
In one embodiment, the methods further comprise quantifying the amount of the gelsolin-like polypeptide in the sample from the subject. In one embodiment, the methods further comprise correlating the amount of gelsolin-like polypeptide in the sample from the mammalian subject to a likelihood of survival of the subject.
In another aspect, the invention provides a method for monitoring kidney failure in a mammalian subject, the method comprising the steps of: (a) determining the level of gelsolin-like polypeptide in the urine of a mammalian subject; (b) comparing the level of gelsolin-like polypeptide in the mammalian subject to a reference level, wherein the reference level comprises a control subject not having kidney failure, and wherein an increase in the level of gelsolin-like polypeptide in the subject compared to the reference level indicates that the mammalian subject has kidney failure.
In another aspect, the invention provides a method for selecting a prophylactic or therapeutic treatment for a mammalian subject, comprising the steps of: (a) determining the level of gelsolin-like polypeptide in the mammalian subject; (b) assigning the subject to a subject class based on the level of gelsolin polypeptide of the subject; and (c) selecting a prophylactic or therapeutic treatment based on the subject class.
In another aspect, the invention provides a method of selecting a mammalian subject for inclusion in a clinical trial for determining the efficacy of a compound to prevent or treat a medical condition associated with altered levels of gelsolin in a mammalian subject, comprising the steps of: (a) measuring the level of gelsolin-like polypeptide in a sample from the mammalian subject; (b) comparing the level of gelsolin in the subject to a reference level, wherein the reference level comprises a control population not having a disease or condition associated with altered levels of gelsolin; and (c) selecting to include the mammalian subject in the clinical trial, wherein a similarity in the level of the gelsolin-like polypeptide in the same from the mammalian subject is similar to the level in the reference standard.
In another aspect, the invention provides an antibody or antigen-binding fragment thereof having the same antigen-binding specificity of antibodies produced by a deposited cell line selected from the group consisting of: CGMCC Accession Nos: 2247 and 2248. In one embodiment, the invention provides an isolated nucleic acids encoding the antibody or an antigen-binding fragment thereof. In one embodiment, the isolated nucleic acids encoding the antibody or an antigen-binding fragment thereof are contained in a vector. The vector may also comprise a promoter operably-linked to the nucleic acid molecule to direct expression of the nucleic acid encoding the antibody or an antigen-binding fragment thereof are contained in a vector. In one aspect, the invention provides host cells comprising the vectors encoding the antibody or an antigen-binding fragment thereof.
In one aspect, the present invention provides a continuous cell line which produces a monoclonal antibody, wherein said monoclonal antibody binds to the same antigenic determinant as an antibody produced by a hybridoma cell line selected from the group consisting of: CGMCC Accession Nos: 2247 and 2248, wherein the cell line is produced by the process of fusing a lymphocyte derived from a mouse immunized with carcinoma cells or an immunogenic determinant thereof and a mouse myeloma cell.
General.
It is to be appreciated that certain aspects, modes, embodiments, variations and features of the invention are described below in various levels of detail in order to provide a substantial understanding of the present invention.
The invention generally provides gelsolin binding agents (e.g., antibodies) which can bind to gelsolin polypeptides. Gelsolin binding agents of the invention are useful, alone or in combination, to detect a gelsolin polypeptide (a.k.a., the target polypeptide) in a test sample as well as in methods to purify gelsolin proteins from a biological sample. Gelsolin binding agents are useful to diagnose a gelsolin-related medical condition in subjects in need thereof. An amino acid sequence of a human gelsolin polypeptide (SEQ ID NO.: 1) is shown in Table 1.
In some embodiments, the gelsolin binding agents (e.g., anti-gelsolin or anti-gelsolin-like antibodies) of the present invention detect the active, or unbound, form of gelsolin. While not wishing to be limited by theory, free and complexed (to actin) gelsolin molecules differ in their functional properties. Although free gelsolin can sever actin filaments, actin-gelsolin in complexes cannot. Gelsolin's severing activity is activated by micromolar Ca2+ and has been shown to be inhibited by phosphatidyl inositol bisphosphate (PIP2) and phosphatidyl inositol monophosphate (PIP). Since extracellular Ca2+ concentrations are at millimolar levels and extracellular fluids do not normally contain PIP or PIP2 in a form that inhibits gelsolin, plasma gelsolin is constitutively active in extracellular fluids.
The various aspects of the present invention further relate to diagnostic methods and kits that use the gelsolin binding agents of the invention to identify individuals predisposed to a medical condition or to classify individuals with regard to drug responsiveness, side effects, or optimal drug dose. In other aspects, the invention provides methods for purifying gelsolin or gelsolin-like polypeptides from a biological sample, including, for example, native human gelsolin from plasma. Accordingly, various particular embodiments that illustrate these aspects follow.
In practicing the present invention, many conventional techniques in molecular biology, protein biochemistry, cell biology, immunology, microbiology and recombinant DNA are used. These techniques are well-known and are explained in, e.g., Current Protocols in Molecular Biology, Vols. I-III, Ausubel, Ed. (1997); Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Ed. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)); DNA Cloning: A Practical Approach, Vols. I and II, Glover, Ed. (1985); Oligonuchotide Synthesis, Gait, Ed. (1984); Nucleic Acid Hybridization, Hames & Higgins, Eds. (1985); Transcription and Translation, Hames & Higgins, Eds. (1984); Animal Cell Culture, Freshney, Ed. (1986); Immobilized Cells and Enzymes (IRL Press, 1986); Perbal, A Practical Guide to Molecular Cloning; the series, Meth. Enzymol., (Academic Press, Inc., 1984); Gene Transfer Vectors for Mammalian Cells, Miller & Calos, Eds. (Cold Spring Harbor Laboratory, New York (1987)); and Meth. Enzymol., Vols. 154 and 155, Wu & Grossman, and Wu, Eds., respectively. Methods to detect and measure levels of polypeptide gene expression products (i.e., gene translation level) are well-known in the art and include the use polypeptide detection methods such as antibody detection and quantification techniques. (See also, Strachan & Read, Human Molecular Genetics, Second Edition. (John Wiley and Sons, Inc., New York (1999).)
Unless defined otherwise, all technical and scientific terms used herein generally have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. As used in this specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the content clearly dictates otherwise. For example, reference to “a cell” includes a combination of two or more cells, and the like. Generally, the nomenclature used herein and the laboratory procedures in cell culture, molecular genetics, organic chemistry, analytical chemistry and nucleic acid chemistry and hybridization described below are those well-known and commonly employed in the art. All references cited herein are incorporated herein by reference in their entireties and for all purposes to the same extent as if each individual publication, patent, or patent application was specifically and individually incorporated by reference in its entirety for all purposes.
Definitions.
The definitions of certain terms as used in this specification are provided below. Definitions of other terms may be found in the Illustrated Dictionary of Immunology, 2nd Edition (Cruse, J. M. and Lewis, R. E., Eds., Boca Raton, Fla.: CRC Press, 1995). Unless indicated otherwise, the term “gelsolin” when used herein refer to the human protein and gene.
As used herein, the “administration” of an agent or drug to a subject or subject includes any route of introducing or delivering to a subject a compound to perform its intended function. Administration can be carried out by any suitable route, including orally, intranasally, parenterally (intravenously, intramuscularly, intraperitoneally, or subcutaneously), rectally, or topically. Administration includes self-administration and the administration by another. It is also to be appreciated that the various modes of treatment or prevention of medical conditions as described are intended to mean “substantial,” which includes total but also less than total treatment or prevention, and wherein some biologically or medically relevant result is achieved.
As used herein, the term “amino acid” includes naturally-occurring amino acids and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally-occurring amino acids. Naturally-occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, γ-carboxyglutamate, and O-phosphoserine. Amino acid analogs refers to compounds that have the same basic chemical structure as a naturally-occurring amino acid, i.e., an α-carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally-occurring amino acid. Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally-occurring amino acid. Amino acids can be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission.
As used herein, the term “antibody” means a polypeptide comprising a framework region from an immunoglobulin gene or fragments thereof that specifically binds and recognizes an antigen, e.g., a gelsolin polypeptide. Use of the term antibody is meant to include whole antibodies, including single-chain whole antibodies, and antigen-binding fragments thereof. The term “antibody” includes bispecific antibodies and multispecific antibodies so long as they exhibit the desired biological activity or function.
As used herein, the term “antibody-related polypeptide” means antigen-binding antibody fragments, including single-chain antibodies, that can comprise the variable region(s) alone, or in combination, with all or part of the following polypeptide elements hinge region, CH1, CH2, and CH3 domains of an antibody molecule. Also included in the invention are any combinations of variable region(s) and hinge region, CH1, CH2, and CH3 domains. Antibody-related molecules useful as binding agents of the invention include, e.g., but are not limited to, Fab, Fab′ and F(ab′)2, Fd, single-chain Fvs (scFv), single-chain antibodies, disulfide-linked Fvs (sdFv) and fragments comprising either a VL or VH domain. Examples include: (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; (ii) a F(ab′)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., Nature 341:544-546, (1989)), which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR). As such “antibody fragments” can comprise a portion of a full length antibody, generally the antigen binding or variable region thereof. Examples of antibody fragments include Fab, Fab′, F(ab′)2, and Fv fragments; diabodies; linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments. Single-chain antibody molecules may comprise a polymer with a number of individual molecules, for example, dimer, trimer or other polymers.
As used herein, the term “CDR-grafted antibody” means an antibody in which at least one CDR of an “acceptor” antibody is replaced by a CDR “graft” from a “donor” antibody possessing a desirable antigen specificity.
As used herein, the term “chimeric antibody” means an antibody in which the Fc constant region of a monoclonal antibody from one species (e.g., a mouse Fc constant region) is replaced, using recombinant DNA techniques, with an Fc constant region from an antibody of another species (e.g., a human Fc constant region). See generally, Robinson et al., PCT/US86/02269; Akira et al., European Patent Application 184,187; Taniguchi, European Patent Application 171,496; Morrison et al., European Patent Application 173,494; Neuberger et al., WO 86/01533; Cabilly et al. U.S. Pat. No. 4,816,567; Cabilly et al., European Patent Application 125,023; Better et al., Science 240:1041-1043 (1988); Liu et al., Proc. Natl. Acad. Sci. USA 84:3439-3443 (1987); Liu et al., J. Immunol 139:3521-3526 (1987); Sun et al., Proc. Natl. Acad. Sci. USA 84:214-218 (1987; Nishimura et al., Cancer Res 47:999-1005 (1987); Wood et al., Nature 314:446-449 (1985); and Shaw et al., J. Natl. Cancer Inst. 80:1553-1559 (1988).
As used herein, the term “clinical response” means any or all of the following: a quantitative measure of the response, no response, and adverse response (i.e., side effects).
As used here, in the term “clinical trial” means any research study designed to collect clinical data on responses to a particular treatment, and includes, but is not limited to phase I, phase II, and phase III clinical trials. Standard methods are used to define the patient population and to enroll subjects.
As used herein, the term “consensus FR” means a framework (FR) antibody region in a consensus immunoglobulin sequence. The FR regions of an antibody do not contact the antigen.
As used herein, the term “diabodies” refers to small antibody fragments with two antigen-binding sites, which fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) in the same polypeptide chain (VH VL). By using a linker that is too short to allow pairing between the two domains on the same chain, the domains are forced to pair with the complementary domains of another chain and create two antigen binding sites. Diabodies are described more fully in, e.g., EP 404,097; WO 93/11161; and Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993).
As used herein, the term “effector cell” means an immune cell which is involved in the effector phase of an immune response, as opposed to the cognitive and activation phases of an immune response. Exemplary immune cells include a cell of a myeloid or lymphoid origin, e.g., lymphocytes (e.g., B cells and T cells including cytolytic T cells (CTLs)), killer cells, natural killer cells, macrophages, monocytes, eosinophils, neutrophils, polymorphonuclear cells, granulocytes, mast cells, and basophils. Effector cells express specific Fc receptors and carry out specific immune functions. An effector cell can induce antibody-dependent cell-mediated cytotoxicity (ADCC), e.g., a neutrophil capable of inducing ADCC. For example, monocytes, macrophages, neutrophils, eosinophils, and lymphocytes which express FcαR are involved in specific killing of target cells and presenting antigens to other components of the immune system, or binding to cells that present antigens. An effector cell can also phagocytose a target antigen, target cell, metastatic cancer cell, or microorganism.
As used herein, the term “epitope” means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents. In one embodiment, an “epitope” of gelsolin is a region in the gelsolin protein to which the gelsolin binding agent of the invention binds. In select embodiments of the invention, this epitope is in the domain spanning amino acid residues from about 321 to about 330, from about 636 to about 645, or from about 661 to 670 of SEQ ID NO.: 1.
To screen for gelsolin binding agents which bind to an epitope, a routine cross-blocking assay such as that described in Antibodies, A Laboratory Manual, Cold Spring Harbor Laboratory, Ed Harlow and David Lane (1988), can be performed. This assay can be used to determine if a gelsolin binding agent binds the same site or epitope as a gelsolin antibody of the invention. Alternatively, or additionally, epitope mapping can be performed by methods known in the art. For example, the antibody sequence can be mutagenized such as by alanine scanning, to identify contact residues. In a different method, peptides corresponding to different regions of gelsolin can be used in competition assays with the test antibodies or with a test antibody and an antibody with a characterized or known epitope.
As used herein, the term “effective amount” or “pharmaceutically effective amount” or “therapeutically effective amount” of a composition, is a quantity sufficient to achieve a desired therapeutic and/or prophylactic effect, e.g., an amount which results in the prevention of, or a decrease in, the symptoms associated with a disease that is being treated, e.g., the diseases or medical conditions associated with target polypeptide (e.g., gelsolin or gelsolin-like polypeptides). The amount of a composition of the invention administered to the subject will depend on the type and severity of the disease and on the characteristics of the individual, such as general health, age, sex, body weight and tolerance to drugs. It will also depend on the degree, severity and type of disease. The skilled artisan will be able to determine appropriate dosages depending on these and other factors. The compositions of the present invention can also be administered in combination with one or more additional therapeutic compounds. In the methods of the present invention, gelsolin may be administered to a subject having decreased gelsolin levels caused by a disease or traumatic condition, thereby increasing the level of plasma gelsolin in the subject. For example, a “therapeutically effective amount” of gelsolin is meant levels in which the toxic effects of free extracellular actin are, at a minimum, ameliorated.
As used herein, “expression” includes but is not limited to one or more of the following: transcription of the gene into precursor mRNA; splicing and other processing of the precursor mRNA to produce mature mRNA; mRNA stability; translation of the mature mRNA into protein (including codon usage and tRNA availability); and glycosylation and/or other modifications of the translation product, if required for proper expression and function.
As used herein, the term “gelsolin” refers to a multifunctional actin binding protein. In mammals, gelsolin comprises two isoforms: cytoplasmic and extracellular variants. Human plasma gelsolin differs from cellular gelsolin only by the addition of about 25 amino acids to the N-terminus of the molecule and both gelsolins are the product of a single gene. Plasma gelsolin has three actin-binding sites and binds with high affinity to either G-actin or F-actin. “Gelsolin” also refers to recombinant forms of the mammalian polypeptide.
As used herein, a “gelsolin-like polypeptide” means a polypeptide that is different from gelsolin polypeptide but which is immunologically-reactive with a gelsolin binding agent of the invention. A gelsolin-like polypeptide may be derived from the same organism or a different organism as a gelsolin polypeptide. A gelsolin-like polypeptide may be encoded by the same gene or a different gene as a gelsolin polypeptide. A gelsolin-like polypeptide may be an immunoreactive fragment (e.g., a C-terminal fragment) of a gelsolin polypeptide.
As used herein, the term “gene” means a segment of DNA that contains all the information for the regulated biosynthesis of an RNA product, including promoters, exons, introns, and other untranslated regions that control expression.
As used herein, the term “human sequence antibody” includes antibodies having variable and constant regions (if present) derived from human germline immunoglobulin sequences. The human sequence antibodies of the invention can include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). Such antibodies can be generated in non-human transgenic animals, e.g., as described in PCT Publication Nos. WO 01/14424 and WO 00/37504. However, the term “human sequence antibody”, as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences (e.g., humanized antibodies).
As used herein, the term “humanized” forms of non-human (e.g., murine) antibodies are chimeric antibodies which contain minimal sequence derived from non-human immunoglobulin. For the most part, humanized antibodies are human immunoglobulins in which hypervariable region residues of the recipient are replaced by hypervariable region residues from a non-human species (donor antibody) such as mouse, rat, rabbit or nonhuman primate having the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the human immunoglobulin are replaced by corresponding non-human residues. Furthermore, humanized antibodies may comprise residues which are not found in the recipient antibody or in the donor antibody. These modifications are made to further refine antibody performance such as binding affinity. Generally, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin sequence although the FR regions may include one or more amino acid substitutions that improve binding affinity. The number of these amino acid substitutions in the FR are typically no more than 6 in the H chain, and in the L chain, no more than 3. The humanized antibody optionally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin. For further details, see Jones et al., Nature 321:522-525 (1986); Reichmann et al., Nature 332:323-329 (1988); and Presta, Curr. Op. Struct. Biol. 2:593-596 (1992).
“Amino acid sequence modification(s)” of the gelsolin antibodies described herein are contemplated. For example, it may be desirable to improve the binding affinity and/or other biological properties of the antibody. Amino acid sequence variants of a gelsolin antibody are prepared by introducing appropriate nucleotide changes into the antibody nucleic acid, or by peptide synthesis. Such modifications include, for example, deletions from, and/or insertions into and/or substitutions of, residues within the amino acid sequences of the gelsolin antibody. Any combination of deletion, insertion, and substitution is made to obtain the antibody of interest, as long as the obtained antibody possesses the desired properties. The modification also includes the change of the pattern of glycosylation of the protein. A useful method for identification of preferred locations for mutagenesis is called “alanine scanning mutagenesis” as described by Cunningham and Wells in Science, 244:1081-1085 (1989). The mutated antibody is then screened for the desired activity. The invention includes antibody variants with one or more amino acid addition, deletion and/or substitution of the amino acid sequence defined by hybridomas GC1C10, CN3E9, GF2D6, GC5D1, GC4A10 having CGMCC Accession Numbers 2114, 2115, 2116, 2247 and 2248, respectively, provided that the antibody variant possesses the desired properties.
As used herein, the term “hypervariable region” refers to the amino acid residues of an antibody which are responsible for antigen-binding. The hypervariable region generally comprises amino acid residues from a “complementarity determining region” or “CDR” (e.g., around about residues 24-34 (L1), 50-56 (L2) and 89-97 (L3) in the VL, and around about 31-35B (H1), 50-65 (H2) and 95-102 (H3) in the VH (Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)) and/or those residues from a “hypervariable loop” (e.g., residues 26-32 (L1), 50-52 (L2) and 91-96 (L3) in the VL, and 26-32 (H1), 52A-55 (H2) and 96-101 (H3) in the VH (Chothia and Lesk J. Mol. Biol. 196:901-917 (1987)).
As used herein, the terms “identical” or percent “identity”, when used in the context of two or more nucleic acids or polypeptide sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (i.e., about 60% identity, preferably 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or higher identity over a specified region (e.g., nucleotide sequence encoding an antibody described herein or amino acid sequence of an antibody described herein), when compared and aligned for maximum correspondence over a comparison window or designated region) as measured using a BLAST or BLAST 2.0 sequence comparison algorithms with default parameters described below, or by manual alignment and visual inspection (see, e.g., NCBI web site). Such sequences are then said to be “substantially identical.” This term also refers to, or can be applied to, the complement of a test sequence. The term also includes sequences that have deletions and/or additions, as well as those that have substitutions. The algorithms can account for gaps and the like. Typically, identity exists over a region that is at least about 25 amino acids or nucleotides in length, or over a region that is at least about 50-100 amino acids or nucleotides in length.
An “isolated” or “purified” polypeptide or biologically-active portion thereof is substantially free of cellular material or other contaminating polypeptides from the cell or tissue source from which the gelsolin binding agent is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. For example, an isolated gelsolin binding agent which is an anti-gelsolin or anti-gelsolin-like antibody would be free of materials that would interfere with diagnostic or therapeutic uses of the agent. Such interfering materials may include enzymes, hormones and other proteinaceous and nonproteinaceous solutes. Alternatively, an isolated gelsolin or gelsolin-like polypeptide, which is immunoractive with a gelsolin binding agent of the invention, would be substantially free of materials that would interfere with diagnostic or therapeutic uses of the polypeptide.
As used herein, the term “intact antibody” means an antibody that has at least two heavy (H) chain polypeptides and two light (L) chain polypeptides interconnected by disulfide bonds. Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as HCVR or VH) and a heavy chain constant region. The heavy chain constant region is comprised of three domains, CH1, CH2 and CH3. Each light chain is comprised of a light chain variable region (abbreviated herein as LCVR or VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs, arranged from amino-terminus to carboxyl-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of the antibodies can mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system.
As used herein, the terms “immunologically cross-reactive” and “immunologically-reactive” are used interchangeably to mean an antigen which is specifically reactive with an antibody which was generated using the same (“immunologically-reactive”) or different (“immunologically cross-reactive”) antigen. Generally, the antigen is gelsolin or gelsolin-like polypeptide, a variant or subsequence thereof.
As used herein, the term “immunologically-reactive conditions” means conditions which allow an antibody, generated to a particular epitope of an antigen, to bind to that epitope to a detectably greater degree than the antibody binds to substantially all other epitopes, generally at least two times above background binding, preferably at least five times above background. Immunologically-reactive conditions are dependent upon the format of the antibody binding reaction and typically are those utilized in immunoassay protocols. See, Harlow & Lane, Antibodies, A Laboratory Manual (Cold Spring Harbor Publications, New York (1988)) for a description of immunoassay formats and conditions.
As used herein, the term “medical condition” includes, but is not limited to, any condition or disease manifested as one or more physical and/or psychological symptoms for which treatment and/or prevention is desirable, and includes previously and newly identified diseases and other disorders. For example, a medical condition may be hepatitis, SLE, cancer, kidney failure, septic shock, stroke, cancer, heart infarction, and side effects of chemotherapy and radiation therapy.
The term “monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. For example, a monoclonal antibody can be an antibody that is derived from a single clone, including any eukaryotic, prokaryotic, or phage clone, and not the method by which it is produced. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to conventional (polyclonal) antibody preparations which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. The modifier “monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. Monoclonal antibodies can be prepared using a wide variety of techniques known in the art including, e.g., but not limited to, hybridoma, recombinant, and phage display technologies. For example, the monoclonal antibodies to be used in accordance with the present invention may be made by the hybridoma method first described by Kohler et al., Nature 256:495 (1975), or may be made by recombinant DNA methods (see, e.g., U.S. Pat. No. 4,816,567). The “monoclonal antibodies” may also be isolated from phage antibody libraries using the techniques described in Clackson et al., Nature 352:624-628 (1991) and Marks et al., J. Mol. Biol. 222:581-597 (1991), for example.
As used herein, the term “pharmaceutically-acceptable carrier” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal compounds, isotonic and absorption delaying compounds, and the like, compatible with pharmaceutical administration.
As used herein, the term “polyclonal antibody” means a preparation of antibodies derived from at least two (2) different antibody-producing cell lines. The use of this term includes preparations of at least two (2) antibodies that contain antibodies that specifically bind to different epitopes or regions of an antigen.
As used herein, the term “polynucleotide” or “nucleic acid” means any RNA or DNA, which may be unmodified or modified RNA or DNA. Polynucleotides include, without limitation, single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions, single- and double-stranded RNA, RNA that is mixture of single- and double-stranded regions, and hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single- and double-stranded regions. In addition, polynucleotide refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The term polynucleotide also includes DNAs or RNAs containing one or more modified bases and DNAs or RNAs with backbones modified for stability or for other reasons. In a particular embodiment, the polynucleotide contains polynucleotide sequences from a gelsolin gene.
As used herein, the terms “polypeptide”, “peptide” and “protein” are used interchangeably herein to mean a polymer comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds, i.e., peptide isosteres. Polypeptide refers to both short chains, commonly referred to as peptides, glycopeptides or oligomers, and to longer chains, generally referred to as proteins. Polypeptides may contain amino acids other than the 20 gene-encoded amino acids. Polypeptides include amino acid sequences modified either by natural processes, such as post-translational processing, or by chemical modification techniques that are well known in the art. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature. In a particular embodiment, the polypeptide contains polypeptide sequences from a gelsolin protein.
As used herein, the term “population” may be any group of at least two individuals. A population may include, e.g., but is not limited to, a reference population, a population group, a family population, a clinical population, and a same sex population.
As used herein, the term “recombinant” when used with reference, e.g., to a cell, or nucleic acid, protein, or vector, indicates that the cell, nucleic acid, protein or vector, has been modified by the introduction of a heterologous nucleic acid or protein or the alteration of a native nucleic acid or protein, or that the material is derived from a cell so modified. Thus, e.g., recombinant cells express genes that are not found within the native (non-recombinant) form of the cell or express native genes that are otherwise abnormally expressed, under expressed or not expressed at all.
As used herein, the term “reference level” refers to an amount or concentration of biomarker (e.g., the level of gelsolin or gelsolin-like polypeptide) which may be of interest for comparative purposes. In one embodiment, a reference level may be the level of at least one biomarker expressed as an average of the level of at least one biomarker taken from a control population of healthy subjects. In another embodiment, the reference level may be the level of at least one biomarker in the same subject at an earlier time, i.e., before the present assay. In even another embodiment, the reference level may be the level of at least one biomarker in the subject prior to receiving a treatment regime.
As used herein, the term “reference standard” is the pattern of expression of one or more genes or proteins observed in either a reference standard population or a single subject prior to administration of a compound. A “reference standard population” means a population characterized by one or more biological characteristics, e.g., drug responsiveness, genotype, haplotype, phenotype, etc.
As used herein, the phrase “salvage receptor binding epitope” refers to an epitope of the Fc region of an IgG molecule (e.g., IgG1, IgG2, IgG3, or IgG4) that is responsible for increasing the in vivo serum half-life of the IgG molecule. To increase the serum half life of the antibody, one may incorporate a salvage receptor binding epitope into the antibody (especially an antibody fragment) as described in U.S. Pat. No. 5,739,277, for example.
As used herein, the term “sample” means sample material derived from or contacted by living cells. The term “sample” is intended to include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. Biological samples of the invention include, e.g., but are not limited to, whole blood, plasma, semen, saliva, tears, urine, fecal material, sweat, buccal, skin, cerebrospinal fluid, and hair. Biological samples can also be obtained from biopsies of internal organs or from cancers. Biological samples can be obtained from subjects for diagnosis or research or can be obtained from undiseased individuals, as controls or for basic research.
As used herein, the terms “single chain antibodies” or “single chain Fv (scFv)” refer to an antibody fusion molecule of the two domains of the Fv fragment, VL and VH. Although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv). See, e.g., Bird et al., Science 242:423-426 (1988); and Huston et al., Proc. Natl. Acad. Sci. USA 85:5879-5883 (1988). Such single chain antibodies are included by reference to the term “antibody” fragments, and can be prepared by recombinant techniques or enzymatic or chemical cleavage of intact antibodies.
As used herein, the term “specific binding” means the contact between a gelsolin binding agent and an antigen with a binding affinity of at least 10−6 M. Preferred binding agents bind with affinities of at least about 10−7 M, and preferably 10−8 M to 10−9 M, 10−10 M, 10−11 M, or 10−12 M.
As used herein, the term “subject” means that the subject is a mammal, such as a human, but can also be an animal, e.g., domestic animals (e.g., dogs, cats and the like), farm animals (e.g., cows, sheep, pigs, horses and the like) and laboratory animals (e.g., monkey, rats, mice, rabbits, guinea pigs and the like).
As used herein, the term “substitution” is one of mutations that is generally used in the art. Those substitution variants have at least one amino acid residue in the gelsolin binding agent replaced by a different residue. The sites of greatest interest for substitutional mutagenesis include the hypervariable regions, but FR alterations are also contemplated. “Conservative substitutions” are shown in the table below under the heading of “preferred substitutions.” If such substitutions result in a change in biological activity, then more substantial changes, denominated “exemplary substitutions” in Table 2, or as further described below in reference to amino acid classes, may be introduced and the products screened.
A particularly preferred type of substitutional variant involves substituting one or more hypervariable region residues of a parent antibody. A convenient way for generating such substitutional variants involves affinity maturation using phage display. Specifically, several hypervariable region sites (e.g., 6-7 sites) are mutated to generate all possible amino acid substitutions at each site. The antibody variants thus generated are displayed in a monovalent fashion from filamentous phage particles as fusions to the gene III product of M13 packaged within each particle. The phage-displayed variants are then screened for their biological activity (e.g., binding affinity) as herein disclosed. In order to identify candidate hypervariable region sites for modification, alanine scanning mutagenesis can be performed to identify hypervariable region residues contributing significantly to antigen binding gelsolin. Alternatively, or additionally, it may be beneficial to analyze a crystal structure of the antigen-antibody complex to identify contact points between the antibody and gelsolin. Such contact residues and neighboring residues are candidates for substitution according to the techniques elaborated herein. Once such variants are generated, the panel of variants is subjected to screening as described herein and antibodies with similar or superior properties in one or more relevant assays may be selected for further development. The invention includes antibody variants with one or more amino acid substitution(s), especially conservative substitutions, to the hypervariable domains of the immunoglobulin heavy or light chain defined by hybridomas GC1C10, GN3E9, GF2D6, GC5D1, and GC4A10 having CGMCC Accession Numbers 2114, 2115, 2116, 2247, and 2248, respectively, provided that the antibody variant possesses the desired properties.
As used herein, a “test sample” means a biological sample obtained from a subject of interest. For example, a test sample can be a biological fluid (e.g., serum or urine), cell or tissue sample, sample from culture or growth media, or isolated nucleic acid or polypeptide derived therefrom.
As used herein, the term “therapeutic agent” is intended to mean a compound that, when present in an effective amount, produces a desired therapeutic effect on a subject in need thereof.
As used herein, the terms “treating” or “treatment” or “alleviation” refers to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent or slow down (lessen) the targeted pathologic condition or disorder. A subject is successfully “treated” for a disorder characterized by decreased gelsolin levels if, after receiving a therapeutic amount of native or recombinant gelsolin according to the methods of the present invention, the subject shows observable and/or measurable reduction in or absence of one or more signs and symptoms of a particular disease or condition associated with altered serum gelsolin levels.
As used herein, the term “variable” refers to the fact that certain segments of the variable domains differ extensively in sequence among antibodies. The V domain mediates antigen binding and define specificity of a particular antibody for its particular antigen. However, the variability is not evenly distributed across the amino acid span of the variable domains. Instead, the V regions consist of relatively invariant stretches called framework regions (FRs) of 15-30 amino acids separated by shorter regions of extreme variability called “hypervariable regions” that are each 9-12 amino acids long. The variable domains of native heavy and light chains each comprise four FRs, largely adopting a β-sheet configuration, connected by three hypervariable regions, which form loops connecting, and in some cases forming part of, the β-sheet structure. The hypervariable regions in each chain are held together in close proximity by the FRs and, with the hypervariable regions from the other chain, contribute to the formation of the antigen-binding site of antibodies. (See Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991).) The constant domains are not involved directly in binding an antibody to an antigen, but exhibit various effector functions, such as participation of the antibody in antibody dependent cellular cytotoxicity (ADCC).
A. Gelsolin Binding Agents.
In one aspect, the present invention provides gelsolin binding agent compositions, a.k.a., the binding agent. In one embodiment, the binding agent of the invention is an intact antibody directed to gelsolin, a gelsolin-like polypeptide, homolog, fragment, or derivative thereof. The binding agents of interest may be ones which bind specifically to free, full-length, active gelsolin, but do not “substantively” (or “substantially”) bind gelsolin which are bound to actin. In such embodiments, the extent of binding of the binding agent of the invention to these proteins will be less than about 10%, preferably, or less than about 5%, or less than about 1%, as determined by fluorescence activated cell sorting (FACS) analysis, ELISA, Western blot, or radioimmunoassay. Alternatively, the binding agents (or combination thereof) may bind a fragment of gelsolin (e.g., a C-terminal fragment), but do not substantially bind to full-length gelsolin.
Prior efforts to generate monoclonal antibodies with defined epitopes (in particular, eptiopes associated with functional gelsolin) have been largely unsuccessful. Gelsolin is a highly conserved protein and highly homologous among species. Gelsolin is also abundant in plasma, which requires that it be well-tolerated by the immune system. Furthermore, due to the fact that gelsolin is a major actin binding protein, the exposed epitopes are limited by the complexing of gelsolin with actin and other plasma proteins. Although some monoclonal antibodies to gelsolin have been produced (see Hiyoshi et al., Biochem Mol Biol Int 32:755-62 (1994)), there is no immunoassay for quantitative measurement of plasma gelsolin.
The present inventors have discovered a strategy to design gelsolin binding agents using various forms of human gelsolin proteins for both immunization and screening, including native gelsolin, recombinant full-length gelsolin, and N- and C-terminal gelsolin fragments. Moreover, the inventors' strategy is designed to break the immune tolerance to common epitopes of human gelsolin using modulators of the immune response. The result of this strategy are gelsolin binding agents with defined epitopes that allow for rapid, accurate, and quantitative assays for plasma gelsolin and/or urine gelsolin-like polypeptides (i.e., gelsolin fragments), which can be used in a clinical setting.
Binding agents of the present invention can be described or specified in terms of the epitope(s) or portion(s) of a polypeptide of the present invention which are recognized or specifically bound by the binding agent, e.g., a region of the gelsolin polypeptide that is located on the surface of the polypeptide (e.g., a hydrophilic region). In one embodiment, the invention provides gelsolin binding agents, e.g., antibodies or antibody-related polypeptides directed to a gelsolin polypeptide (a.k.a., a target polypeptide) comprising one or more amino acid sequences selected from the group consisting of: FAQGALKSED (SEQ ID NO.: 2), SEPDGFWEAL (SEQ ID NO.: 3), and ACSNKIGRFV (SEQ ID NO.: 4).
In select embodiments, the invention provides the gelsolin binding agents summarized in Table 3.
Deposits of biological materials associated with the gelsolin binding agents summarized in Table 3 (above) were made with the China General Microbiological Culture Collection Center (CGMCC), China Committee for Culture Collection of Microorganisms, P.O. Box 2714, Beijing 100080, The People's Republic of China as detailed in Table 4 below.
In another embodiment, the present invention affords a method of elucidating other epitopes of gelsolin, which can be used for generation of an antibody having the desired characteristics of binding to active gelsolin, but not gelsolin bound to actin. The binding agents directed against the epitope may have a differing variable or CDR region but should have the binding and functional characteristics of the antibodies of the present invention. As a means for targeting antibody production, hydropathy plots showing regions of hydrophilicity and hydrophobicity can be generated by any method well known in the art, including, e.g., the Kyte Doolittle or the Hopp Woods methods, either with or without Fourier transformation. (See, e.g., Hopp and Woods, Proc. Nat. Acad. Sci. USA 78: 3824-3828 (1981); Kyte and Doolittle, J. Mol. Biol. 157:105-142 (1982).) The epitope(s) or polypeptide portion(s) can be specified as described herein, e.g., by N-terminal and C-terminal positions, by size in contiguous amino acid residues. The present invention includes binding agents that specifically bind polypeptides of the present invention, and allows for the exclusion of the same. The present invention includes binding agents that specifically bind epitopes which are conformational epitopes or nonconformational epitopes. As noted above, conformational epitopes or nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.
Binding agents of the present invention can also be described or specified in terms of their cross-reactivity. Binding agents that do not bind any other analog, ortholog, or homolog of the target polypeptide of the present invention are included. Binding agents that do not bind polypeptides with less than 95%, less than 90%, less than 85%, less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 55%, and less than 50% identity (as calculated using methods known in the art and described herein) to a polypeptide of the present invention are also included in the present invention. Further included in the present invention are binding agents which only bind polypeptides encoded by polynucleotides which hybridize to a polynucleotide of the present invention under stringent hybridization conditions (as described herein).
Binding agents of the present invention can also be described or specified in terms of their binding affinity. Preferred binding affinities include those with a dissociation constant or Kd less than 5×10−6 M, 10−6 M, 5×10−7 M, 10−7 M, 5×10−8 M, 10−8 M, 5×10−9 M, 10−9 M, 5×10−10 M, 10−10 M, 5×10−11 M, 10−11 M, 5×10−12 M, 10−12 M, 5×10−13 M, 10−13 M, 5×10−14 M, 10−14 M, 5×10−15 M, and 10−15 M. In one embodiment, the invention provides gelsolin binding agents that at least bind human gelsolin with a Kd value of no higher than 1×10−8, preferably a Kd value no higher than about 1×10−9.
Gelsolin binding agents within the scope of the present invention include, e.g., but are not limited to, monoclonal, polyclonal, chimeric, humanized, diabody, and human monoclonal and human polyclonal antibodies which specifically bind the target polypeptide, a homolog, derivative or a fragment thereof. The antibodies useful as binding agents of the present invention include, e.g., but are not limited to, IgG (including IgG1, IgG2, IgG3, and IgG4), IgA (including IgA1 and IgA2), IgD, IgE, or IgM, and IgY.
In another embodiment, the binding agent of the invention is an antibody-related polypeptide directed to gelsolin polypeptide, homolog or derivative thereof. Typically, the antigen-binding region of a binding agent, e.g., the anti-gelsolin binding region, will be most critical in specificity and affinity of binding of the binding agent of the invention. In some embodiments, the gelsolin binding agent is an anti-gelsolin polypeptide antibody, such as an anti-gelsolin polypeptide monoclonal antibody, an anti-gelsolin polypeptide chimeric antibody, and an anti-gelsolin polypeptide humanized antibody which have been modified by, e.g., deleting, adding, or substituting portions of the antibody.
In one embodiment, selection of antibodies that are specific to a particular domain of a gelsolin polypeptide is facilitated by generation of hybridomas that bind to the fragment of a gelsolin polypeptide possessing such a domain. Thus, gelsolin binding agents which are antibodies that are specific for a desired domain within a gelsolin polypeptide, or derivatives, fragments, analogs or homologs thereof, are also provided herein.
The present invention further includes antibodies which are anti-idiotypic to the binding agents of the present invention. The binding agents of the present invention can be monospecific, bispecific, trispecific or of greater multispecificity. Multispecific binding agents can be specific for different epitopes of a gelsolin polypeptide of the present invention or can be specific for both a gelsolin polypeptide of the present invention as well as for heterologous compositions, such as a heterologous polypeptide or solid support material. See, e.g., WO 93/17715; WO 92/08802; WO 91/00360; WO 92/05793; Tutt et al., J. Immunol. 147:60-69 (1991); U.S. Pat. Nos. 5,573,920, 4,474,893, 5,601,819, 4,714,681, 4,925,648; 6,106,835; Kostelny et al., J. Immunol. 148:1547-1553 (1992). The binding agents of the invention can be from any animal origin, including birds and mammals. For example, the binding agents may be from human, marine, rabbit, goat, guinea pig, camel, horse, or chicken.
The binding agents of the present invention can be used either alone or in combination with other compositions. For example, the gelsolin binding agents of the invention can be used in combination with one or more anti-gelsolin antibodies known in the art, e.g., but not limited to antibody GS-2C4. (Sigma-Aldrich, Cat. No. G4896; Afify and Werness. Appl. Immunohistochem. 6:30 (1998).)
The gelsolin binding agents of the present invention can further be recombinantly fused to a heterologous polypeptide at the N- or C-terminus or chemically conjugated (including covalently and non-covalently conjugations) to polypeptides or other compositions. For example, gelsolin binding agents of the present invention can be recombinantly fused or conjugated to molecules useful as labels in detection assays and effector molecules such as heterologous polypeptides, drugs, or toxins. See, e.g., WO 92/08495; WO 91/14438; WO 89/12624; U.S. Pat. No. 5,314,995; and EP 0 396 387.
B. Methods of Preparing a Gelsolin-Binding Agents of the Invention
General Overview. Techniques for generating binding agents directed to target polypeptides are well known to those skilled in the art. Examples of such techniques include, but are not limited to, e.g., those involving display libraries, xeno or humab mice, hybridomas, and the like. Target polypeptides within the scope of the present invention include any polypeptide or polypeptide derivative which is capable of exhibiting antigenicity. Examples include, but are not limited to, gelsolin and fragments thereof.
It should be understood that not only are naturally-occurring antibodies suitable as binding agents for use in accordance with the present disclosure, but recombinantly engineered antibodies and antibody fragments, e.g., antibody-related polypeptides, which are directed to gelsolin polypeptide and fragments thereof are also suitable.
Binding agents, e.g., anti-gelsolin antibodies, that can be subjected to the techniques set forth herein include monoclonal and polyclonal antibodies, and antibody fragments such as Fab, Fab′, F(ab′)2, Fd, scFv, diabodies, antibody light chains, antibody heavy chains and/or antibody fragments. Methods useful for the high yield production of antibody Fv-containing polypeptides, e.g., Fab′ and F(ab′)2 antibody fragments have been described. See U.S. Pat. No. 5,648,237.
Generally, a binding agent is obtained from an originating species. More particularly, the nucleic acid or amino acid sequence of the variable portion of the light chain, heavy chain or both, of an originating species antibody having specificity for a target polypeptide antigen is obtained. Originating species is any species which was useful to generate the binding agent of the invention or library of binding agents, e.g., rat, mice, rabbit, chicken, monkey, human, and the like.
In some embodiments, gelsolin binding agents are anti-gelsolin antibodies. Phage or phagemid display technologies are useful techniques to derive the binding agents of the present invention. Anti-gelsolin antibodies useful in the present invention are “human antibodies,” (e.g., antibodies isolated from a human) or “human sequence antibodies.” Human antibodies can be made by a variety of methods known in the art including phage display methods. See also, U.S. Pat. Nos. 4,444,887, 4,716,111, 5,545,806, and 5,814,318; and WO 98/46645, WO 98/50433, WO 98/24893, WO 98/16654, WO 96/34096, WO 96/33735, and WO 91/10741. Methods useful for the identification of nucleic acid sequences encoding members of multimeric polypeptide complex by screening polyphage particles have been described. Rudert et al., U.S. Pat. No. 6,667,150. Also, recombinant immunoglobulins can be produced. Cabilly, U.S. Pat. No. 4,816,567; Cabilly et al., U.S. Pat. No. 6,331,415 and Queen et al., Proc. Natl. Acad. Sci. USA 86:10029-10033 (1989). Techniques for generating and cloning monoclonal antibodies are well known to those skilled in the art. The gelsolin binding agents of the invention suitably have a high immunoreactivity, that is, percentages of antibodies molecules that are correctly folded so that they can specifically bind their target antigen. Expression of sequences encoding binding agents, e.g., antibodies of the invention, can be carried out in E. coli as described below. Such expression usually results in immunoreactivity of at least 80%, 90%, 95% or 99%.
Certain truncations of these proteins or genes perform the regulatory or enzymatic functions of the full sequence protein or gene. For example, the nucleic acid sequences coding therefore can be altered by substitutions, additions, deletions or multimeric expression that provide for functionally equivalent proteins or genes. Due to the degeneracy of nucleic acid coding sequences, other sequences which encode substantially the same amino acid sequences as those of the naturally occurring proteins may be used in the practice of the present invention. These include, but are not limited to, nucleic acid sequences including all or portions of the nucleic acid sequences encoding the above polypeptides, which are altered by the substitution of different codons that encode a functionally equivalent amino acid residue within the sequence, thus producing a silent change. It is appreciated that the nucleotide sequence of an immunoglobulin according to the present invention tolerates sequence homology variations of up to 25% as calculated by standard methods (“Current Methods in Sequence Comparison and Analysis,” Macromolecule Sequencing and Synthesis, Selected Methods and Applications, Alan R Liss, Inc., 127-149, 1998) so long as such a variant forms an operative antibody which recognizes gelsolin or gelsolin-like polypeptides. For example, one or more amino acid residues within a polypeptide sequence can be substituted by another amino acid of a similar polarity which acts as a functional equivalent, resulting in a silent alteration. Substitutes for an amino acid within the sequence may be selected from other members of the class to which the amino acid belongs. For example, the nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan and methionine. The polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine. The positively charged (basic) amino acids include arginine, lysine and histidine. The negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Also included within the scope of the present invention are proteins or fragments or derivatives thereof which are differentially modified during or after translation, e.g., by glycosylation, protolytic cleavage, linkage to an antibody molecule or other cellular ligands, etc. Additionally, an inhibitor encoding nucleic acid sequence can be mutated in vitro or in vivo to create and/or destroy translation, initiation, and/or termination sequences or to create variations in coding regions and/or form new restriction endonuclease sites or destroy pre-existing ones, to facilitate further in vitro modification. Any technique for mutagenesis known in the art can be used, including but not limited to in vitro site directed mutagenesis, J. Biol. Chem. 253:6551 (1978), use of Tab linkers (Pharmacia), and the like.
Preparation of Polyclonal Antisera and Immunogens.
Methods of generating antibodies or antibody fragments of the invention typically include immunizing a subject (generally a non-human subject such as a mouse or rabbit) with a purified gelsolin or gelsolin-like polypeptide or homolog or fragment thereof or with a cell expressing the gelsolin or gelsolin-like polypeptide or homolog or fragment thereof. Any immunogenic portion of the gelsolin polypeptide can be employed as the immunogen. An appropriate immunogenic preparation can contain, e.g., a recombinantly-expressed gelsolin polypeptide or a chemically-synthesized gelsolin polypeptide. An isolated gelsolin polypeptide, or a portion or fragment thereof, can be used as an immunogen to generate a gelsolin binding agent that binds to the gelsolin polypeptide, or a portion or fragment using standard techniques for polyclonal and monoclonal antibody preparation. The full-length gelsolin polypeptide can be used or, alternatively, the invention provides for the use of the gelsolin polypeptide fragments as immunogens. The gelsolin polypeptide comprises at least four amino acid residues of the amino acid sequence shown in SEQ ID NO.: 1, and encompasses an epitope of the gelsolin polypeptide such that an antibody raised against the peptide forms a specific immune complex with the gelsolin polypeptide. The antigenic peptide may comprise at least 5, 8, 10, 15, 20, or 30 amino acid residues. Longer antigenic peptides are sometimes preferable over shorter antigenic peptides, depending on use and according to methods well known to those skilled in the art. Typically, the immunogen will be at least about 8 amino acyl residues in length, or at least about 10 acyl residues in length. Multimers of a given epitope are sometimes more effective than a monomer.
If needed, the immunogenicity of the gelsolin polypeptide (or fragment thereof) can be increased by fusion or conjugation to a hapten such as keyhole limpet hemocyanin (KLH) or ovalbumin (OVA). Many such haptens are known in the art. One can also combine the gelsolin polypeptide with a conventional adjuvant such as Freund's complete or incomplete adjuvant to increase the subject's immune reaction to the polypeptide. Various adjuvants used to increase the immunological response include, but are not limited to, Freund's (complete and incomplete), mineral gels (e.g., aluminum hydroxide), surface active substances (e.g., lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, dinitrophenol, etc.), human adjuvants such as Bacille Calmette-Guerin and Corynebacterium parvum, or similar immunostimulatory compounds. These techniques are standard in the art.
For convenience, immune responses are often described in the present invention as being either “primary” or “secondary” immune responses. A primary immune response, which is also described as a “protective” immune response, refers to an immune response produced in an individual as a result of some initial exposure (e.g., the initial “immunization”) to a particular antigen, e.g., a gelsolin polypeptide. Such an immunization can occur, e.g., as the result of some natural exposure to the antigen (e.g., from initial infection by some pathogen that exhibits or presents the antigen) or from antigen presented by cancer cells of some tumor in the individual (e.g., malignant melanoma). Alternatively, the immunization can occur as a result of vaccinating the individual with a vaccine containing the antigen. For example, the vaccine can be a gelsolin vaccine comprising one or more antigens from a gelsolin polypeptide or a gelsolin-like polypeptide.
A primary immune response can become weakened or attenuated over time and can even disappear or at least become so attenuated that it cannot be detected. Accordingly, the present invention also relates to a “secondary” immune response, which is also described here as a “memory immune response.” The term secondary immune response refers to an immune response elicited in an individual after a primary immune response has already been produced.
Thus, a secondary or immune response can be elicited, e.g., to enhance an existing immune response that has become weakened or attenuated, or to recreate a previous immune response that has either disappeared or can no longer be detected. The secondary or memory immune response can be either a humoral (antibody) response or a cellular response. A secondary or memory humoral response occurs upon stimulation of memory B cells that were generated at the first presentation of the antigen. Delayed type hypersensitivity (DTH) reactions are a type of cellular secondary or memory immune response that are mediated by CD4+ cells. A first exposure to an antigen primes the immune system and additional exposure(s) results in a DTH.
Following appropriate immunization, the gelsolin binding agent, e.g., anti-gelsolin polyclonal antibody can be prepared from the subject's serum. If desired, the antibody molecules directed against the gelsolin polypeptide can be isolated from the mammal (e.g., from the blood) and further purified by well known techniques, such as protein A chromatography to obtain the IgG fraction.
Monoclonal Antibody.
In one embodiment, the binding agent is an anti-gelsolin monoclonal antibody. For example, in some embodiments, the anti-gelsolin monoclonal antibody may be a human or a mouse anti-gelsolin monoclonal antibody. For preparation of monoclonal antibodies directed towards a particular gelsolin polypeptide, or derivatives, fragments, analogs or homologs thereof, any technique that provides for the production of antibody molecules by continuous cell line culture can be utilized. Such techniques include, but are not limited to, the hybridoma technique (see, e.g., Kohler & Milstein, Nature 256:495-497 (1975)); the trioma technique; the human B-cell hybridoma technique (see, e.g., Kozbor, et al., Immunol. Today 4:72 (1983)) and the EBV hybridoma technique to produce human monoclonal antibodies. (See, e.g., Cole, et al., In: Monoclonal Antibodies And Cancer Therapy, Alan R Liss, Inc., 77-96 (1985).) Human monoclonal antibodies can be utilized in the practice of the invention and can be produced by using human hybridomas (see, e.g., Cote, et al., Proc. Natl. Acad. Sci. USA 80:2026-2030 (1983)) or by transforming human B-cells with Epstein Barr Virus in vitro. (See, e.g., Cole, et al., In: MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R Liss, Inc., 77-96 (1985).) For example, a population of nucleic acids that encode regions of antibodies can be isolated. PCR utilizing primers derived from sequences encoding conserved regions of antibodies is used to amplify sequences encoding portions of antibodies from the population and then reconstruct DNAs encoding antibodies or fragments thereof, such as variable domains, from the amplified sequences. Such amplified sequences also can be fused to DNAs encoding other proteins—e.g., a bacteriophage coat, or a bacterial cell surface protein—for expression and display of the fusion polypeptides on phage or bacteria. Amplified sequences can then be expressed and further selected or isolated based, e.g., on the affinity of the expressed antibody or fragment thereof for an antigen or epitope present on the gelsolin polypeptide. Alternatively, hybridomas expressing anti-gelsolin monoclonal antibodies can be prepared by immunizing a subject and then isolating hybridomas from the subject's spleen using routine methods. See, e.g., Milstein et al., Galfre and Milstein, Methods Enzymol 73:3-46 (1981). Screening the hybridomas using standard methods will produce monoclonal antibodies of varying specificity (i.e., for different epitopes) and affinity. A selected monoclonal antibody with the desired properties, e.g., gelsolin binding, can be used as expressed by the hybridoma, it can be bound to a molecule such as polyethylene glycol (PEG) to alter its properties, or a cDNA encoding it can be isolated, sequenced and manipulated in various ways. Synthetic dendromeric trees can be added a reactive amino acid side chains, e.g., lysine to enhance the immunogenic properties of the gelsolin polypeptide. Also, CPG-dinucleotide technique can be used to enhance the immunogenic properties of the gelsolin polypeptide. Other manipulations include substituting or deleting particular amino acyl residues that contribute to instability of the antibody during storage or after administration to a subject, and affinity maturation techniques to improve affinity of the antibody of the gelsolin polypeptide.
Hybridoma Technique.
In one embodiment, the binding agent of the invention is an anti-gelsolin monoclonal antibody produced by a hybridoma which includes a B cell obtained from a transgenic non-human animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell. Hybridoma techniques include those known in the art and taught in Harlow et al., Antibodies: A Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. 349 (1988); Hammerling et al., Monoclonal Antibodies And T-Cell Hybridomas, 563-681 (1981). Other methods for producing hybridomas and monoclonal antibodies are well known to those of skill in the art.
Phage Display Technique.
As noted above, the binding agents of the present invention can be produced through the application of recombinant DNA and phage display technology. For example, binding agents of the invention, e.g., anti-gelsolin antibodies, can be prepared using various phage display methods known in the art. In phage display methods, functional antibody domains are displayed on the surface of a phage particle which carries polynucleotide sequences encoding them. Phage with a desired binding property are selected from a repertoire or combinatorial antibody library (e.g., human or murine) by selecting directly with antigen, typically antigen bound or captured to a solid surface or bead. Phage used in these methods are typically filamentous phage including fd and M13 with Fab, Fv or disulfide stabilized Fv antibody domains are recombinantly fused to either the phage gene III or gene VIII protein. In addition, methods can be adapted for the construction of Fab expression libraries (see, e.g., Huse, et al., Science 246:1275-1281, (1989)), to allow rapid and effective identification of monoclonal Fab fragments with the desired specificity for a gelsolin polypeptide, e.g., a polypeptide or derivatives, fragments, analogs or homologs thereof. Other examples of phage display methods that can be used to make the binding agents of the present invention include those disclosed in Huston et al., Proc. Natl. Acad. Sci. USA. 85:5879-5883 (1988); Chaudhary et al., Proc. Natl. Acad. Sci. USA. 87:1066-1070 (1990); Brinkman et al., J. Immunol. Methods 182:41-50 (1995); Ames et al., J. Immunol. Methods 184:177-186 (1995); Kettleborough et al., Eur. J. Immunol. 24:952-958 (1994); Persic et al., Gene 187:9-18 (1997; Burton et al., Advances in Immunology 57:191-280 (1994); PCT/GB91/01134; WO 90/02809; WO 91/10737; WO 92/01047; WO 92/18619; WO 93/11236; WO 95/15982; WO 95/20401; WO 96/06213; WO 92/01047 (Medical Research Council et al.); WO 97/08320 (Morphosys); WO 92/01047 (CAT/MRC); WO 91/17271 (Affymax); and U.S. Pat. Nos. 5,698,426, 5,223,409, 5,403,484, 5,580,717, 5,427,908, 5,750,753, 5,821,047, 5,571,698, 5,427,908, 5,516,637, 5,780,225, 5,658,727 and 5,733,743. Methods useful for displaying polypeptides on the surface of bacteriophage particles by attaching the polypeptides via disulfide bonds have been described by Lohning, U.S. Pat. No. 6,753,136. As described in the above references, after phage selection, the antibody coding regions from the phage can be isolated and used to generate whole antibodies, including human antibodies, or any other desired antigen binding fragment, and expressed in any desired host including mammalian cells, insect cells, plant cells, yeast, and bacteria. For example, techniques to recombinantly produce Fab, Fab′ and F(ab′)2 fragments can also be employed using methods known in the art such as those disclosed in WO 92/22324; Mullinax et al., BioTechniques 12:864-869 (1992); and Sawai et al., AJRI 34:26-34 (1995); and Better et al., Science 240:1041-1043 (1988).
Generally, hybrid antibodies or hybrid antibody fragments that are cloned into a display vector can be selected against the appropriate antigen in order to identify variants that maintained good binding activity, because the antibody or antibody fragment will be present on the surface of the phage or phagemid particle. See e.g., Barbas III et al., Phage Display, A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 2001). However, other vector formats could be used for this process, such as cloning the antibody fragment library into a lytic phage vector (modified T7 or Lambda Zap systems) for selection and/or screening.
Expression of Recombinant Gelsolin-Binding Agent. As noted above, the binding agents of the present invention can be produced through the application of recombinant DNA technology. Recombinant polynucleotide constructs encoding a gelsolin binding agent of the present invention typically include an expression control sequence operably-linked to the coding sequences of anti-gelsolin antibody chains, including naturally-associated or heterologous promoter regions. As such, another aspect of the invention includes vectors containing one or more nucleic acid sequences encoding a gelsolin binding agent of the present invention. For recombinant expression of one or more the polypeptides of the invention, the nucleic acid containing all or a portion of the nucleotide sequence encoding the gelsolin binding agent is inserted into an appropriate cloning vector, or an expression vector (i.e., a vector that contains the necessary elements for the transcription and translation of the inserted polypeptide coding sequence) by recombinant DNA techniques well known in the art and as detailed below. Methods for producing diverse populations of vectors have been described by Lerner et al., U.S. Pat. Nos. 6,291,160; 6,680,192.
In general, expression vectors useful in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors that are not technically plasmids, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions. Such viral vectors permit infection of a subject and expression in that subject of a compound. The expression control sequences are typically eukaryotic promoter systems in vectors capable of transforming or transfecting eukaryotic host cells. Once the vector has been incorporated into the appropriate host, the host is maintained under conditions suitable for high level expression of the nucleotide sequences encoding the gelsolin binding agent, and the collection and purification of the gelsolin binding agent, e.g., cross-reacting anti-gelsolin antibodies. See, generally, U.S. Application No. 20020199213. These expression vectors are typically replicable in the host organisms either as episomes or as an integral part of the host chromosomal DNA. Commonly, expression vectors contain selection markers, e.g., ampicillin-resistance or hygromycin-resistance, to permit detection of those cells transformed with the desired DNA sequences. Vectors can also encode signal peptide, e.g., pectate lyase, useful to direct the secretion of extracellular antibody fragments. See U.S. Pat. No. 5,576,195.
The recombinant expression vectors comprise a nucleic acid encoding a compound with gelsolin binding properties in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression that is operatively-linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, “operably-linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner that allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term “regulatory sequence” is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, e.g., in Goeddel, Gene Expression Technology: Methods In Enzymology 185, Academic Press, San Diego, Calif. (1990). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cell and those that direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences). It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc. Typical regulatory sequences useful as promoters of recombinant polypeptide expression (e.g., gelsolin binding agents), include, e.g., but are not limited to, 3-phosphoglycerate kinase and other glycolytic enzymes. Inducible yeast promoters include, among others, promoters from alcohol dehydrogenase, isocytochrome C, and enzymes responsible for maltose and galactose utilization. In one embodiment, a polynucleotide encoding a gelsolin binding agent of the invention is operably-linked to an ara B promoter and expressible in a host cell. See U.S. Pat. No. 5,028,530. The expression vectors of the invention can be introduced into host cells to thereby produce polypeptides or peptides, including fusion polypeptides, encoded by nucleic acids as described herein (e.g., gelsolin binding agents, etc.).
Another aspect of the invention pertains to gelsolin binding agent-expressing host cells, which contain a nucleic acid encoding one or more gelsolin binding agents. The recombinant expression vectors of the invention can be designed for expression of a gelsolin binding agent in prokaryotic or eukaryotic cells. For example, a gelsolin binding agent can be expressed in bacterial cells such as Escherichia coli, insect cells (using baculovirus expression vectors), fungal cells, e.g., yeast, yeast cells or mammalian cells. Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods In Enzymology 185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, e.g., using T7 promoter regulatory sequences and T7 polymerase. Methods useful for the preparation screening of polypeptides having predetermined property, e.g., gelsolin binding agents, via expression of stochastically generated polynucleotide sequences has been described. See U.S. Pat. Nos. 5,763,192; 5,723,323; 5,814,476; 5,817,483; 5,824,514; 5,976,862; 6,492,107; 6,569,641.
Expression of polypeptides in prokaryotes is most often carried out in E. coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion polypeptides. Fusion vectors add a number of amino acids to a polypeptide encoded therein, usually to the amino terminus of the recombinant polypeptide. Such fusion vectors typically serve three purposes: (i) to increase expression of recombinant polypeptide; (ii) to increase the solubility of the recombinant polypeptide; and (iii) to aid in the purification of the recombinant polypeptide by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant polypeptide to enable separation of the recombinant polypeptide from the fusion moiety subsequent to purification of the fusion polypeptide. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith and Johnson, Gene 67:31-40 (1988)), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) that fuse glutathione S-transferase (GST), maltose E binding polypeptide, or polypeptide A, respectively, to the target recombinant polypeptide.
Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amrann et al., Gene 69:301-315 (1988) and pET 11d (Studier et al., Gene Expression Technology: Methods In Enzymology 185, Academic Press, San Diego, Calif. 60-89 (1990)). Methods for targeted assembly of distinct active peptide or protein domains to yield multifunctional polypeptides via polypeptide fusion has been described by Pack et al., U.S. Pat. Nos. 6,294,353; 6,692,935. One strategy to maximize recombinant polypeptide expression, e.g., a gelsolin binding agent, in E. coli is to express the polypeptide in host bacteria with an impaired capacity to proteolytically cleave the recombinant polypeptide. See, e.g., Gottesman, Gene Expression Technology: Methods In Enzymology 185, Academic Press, San Diego, Calif. 119-128 (1990). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the expression host, e.g., E. coli. (See, e.g., Wada, et al., Nucl. Acids Res. 20:2111-2118 (1992).) Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.
In another embodiment, the gelsolin binding agent expression vector is a yeast expression vector. Examples of vectors for expression in yeast Saccharomyces cerivisae include pYepSec1 (Baldari, et al., EMBO J. 6:229-234 (1987)), pMFa (Kurjan and Herskowitz, Cell 30:933-943 (1982)), pJRY88 (Schultz et al., Gene 54:113-123 (1987)), pYES2 (Invitrogen Corporation, San Diego, Calif.), and picZ (InVitrogen Corp, San Diego, Calif.). Alternatively, a gelsolin binding agent can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of polypeptides, e.g., gelsolin binding agents, in cultured insect cells (e.g., SF9 cells), include the pAc series (Smith, et al., Mol. Cell. Biol. 3:2156-2165 (1983)), and the pVL series (Lucklow and Summers, Virology 170:31-39 (1989)).
In yet another embodiment, a nucleic acid encoding a gelsolin binding agent of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include, e.g., but are not limited to, pCDM8 (Seed, Nature 329:840 (1987)), and pMT2PC (Kaufman, et al., EMBO J. 6:187-195 (1987)). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenovirus 2, cytomegalovirus, and simian virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells useful for expression of the gelsolin binding agents of the present invention. See, e.g., Chapters 16 and 17 of Sambrook, et al., Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).
In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert, et al., Genes Dev. 1:268-277 (1987)), lymphoid-specific promoters (Calame and Eaton, Adv. Immunol. 43:235-275 (1988)), in particular promoters of T cell receptors (Winoto and Baltimore, EMBO J. 8:729-733 (1989)), and immunoglobulins (Banerji, et al., Cell 33:729-740 (1993); Queen and Baltimore, Cell 33:741-748 (1983)), neuron-specific promoters (e.g., the neurofilament promoter; Byrne and Ruddle, Proc. Natl. Acad. Sci. USA 86:5473-5477 (1989)), pancreas-specific promoters (Edlund, et al., Science 230:912-916 (1985)), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, e.g., the murine hox promoters (Kessel and Gruss, Science 249:374-379 (1990)), and the α-fetoprotein promoter (Campes and Tilghman, Genes Dev. 3:537-546 (1989)).
Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms “host cell” and “recombinant host cell” are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.
A host cell can be any prokaryotic or eukaryotic cell. For example, a gelsolin binding agent can be expressed in bacterial cells such as E. coli, insect cells, yeast or mammalian cells. Mammalian cells are one host for expressing nucleotide segments encoding immunoglobulins or fragments thereof. See Winnacker, From Genes To Clones, (VCH Publishers, New York (1987)). A number of suitable host cell lines capable of secreting intact heterologous proteins have been developed in the art, and include Chinese hamster ovary (CHO) cell lines, various COS cell lines, HeLa cells, L cells and myeloma cell lines. Preferably, the cells are nonhuman. Expression vectors for these cells can include expression control sequences, such as an origin of replication, a promoter, an enhancer, and necessary processing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcriptional terminator sequences. Queen et al., Immunol. Rev. 89:49 (1986). Preferred expression control sequences are promoters derived from endogenous genes, cytomegalovirus, SV40, adenovirus, bovine papillomavirus, and the like. Co et al., J Immunol. 148:1149 (1992). Other suitable host cells are known to those skilled in the art.
Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection” are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, or electroporation, biolistics or viral-based transfection can be used for other cellular hosts. Other methods used to transform mammalian cells include the use of polybrene, protoplast fusion, liposomes, electroporation, and microinjection (see generally, Sambrook et al., Molecular Cloning: A Laboratory Manual). Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)), and other laboratory manuals. The vectors containing the DNA segments of interest can be transferred into the host cell by well known methods, depending on the type of cellular host.
For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker (e.g., resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Various selectable markers include those that confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding the gelsolin binding agent or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drug selection (e.g., cells that have incorporated the selectable marker gene will survive, while the other cells die).
A host cell that includes a gelsolin binding agent of the present invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) recombinant gelsolin binding agent. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding the gelsolin binding agent has been introduced) in a suitable medium such that the gelsolin binding agent is produced. In another embodiment, the method further comprises the step of isolating the gelsolin binding agent from the medium or the host cell. Once expressed, collections of the gelsolin binding agents, e.g., the anti-gelsolin antibodies or the anti-gelsolin antibody-related polypeptides are purified from culture media and host cells. The gelsolin binding agents can be purified according to standard procedures of the art, including HPLC purification, column chromatography, gel electrophoresis and the like. In one embodiment, the gelsolin binding agent is produced in a host organism by the method of Boss et al., U.S. Pat. No. 4,816,397. Usually, anti-gelsolin antibody chains are expressed with signal sequences and are thus released to the culture media. However, if the anti-gelsolin antibody chains are not naturally secreted by host cells, the anti-gelsolin antibody chains can be released by treatment with mild detergent. Purification of recombinant polypeptides is well known in the art and include ammonium sulfate precipitation, affinity chromatography purification technique, column chromatography, ion exchange purification technique, gel electrophoresis and the like (see generally Scopes, Protein Purification (Springer-Verlag, N.Y. (1982)).
Polynucleotides encoding gelsolin binding agents, e.g., the anti-gelsolin antibody coding sequences, can be incorporated in transgenes for introduction into the genome of a transgenic animal and subsequent expression in the milk of the transgenic animal. See, e.g., U.S. Pat. Nos. 5,741,957, 5,304,489, and 5,849,992. Suitable transgenes include coding sequences for light and/or heavy chains in operable linkage with a promoter and enhancer from a mammary gland specific gene, such as casein or β-lactoglobulin. For production of transgenic animals, transgenes can be microinjected into fertilized oocytes, or can be incorporated into the genome of embryonic stem cells, and the nuclei of such cells transferred into enucleated oocytes.
Single Chain Antibodies.
In one embodiment, the binding agent of the invention is a single chain anti-gelsolin antibody. According to the invention, techniques can be adapted for the production of single-chain antibodies specific to a gelsolin polypeptide (see, e.g., U.S. Pat. No. 4,946,778). Examples of techniques which can be used to produce single-chain Fvs and antibodies of the invention include those described in U.S. Pat. Nos. 4,946,778 and 5,258,498; Huston et al., Methods in Enzymology, 203:46-88 (1991); Shu, L. et al., Proc. Natl. Acad. Sci. USA, 90:7995-7999 (1993); and Skerra et al., Science 240:1038-1040 (1988).
Chimeric and Humanized Antibodies.
In one embodiment, the binding agent of the invention is a chimeric anti-gelsolin antibody. In one embodiment, the binding agent of the invention is a humanized anti-gelsolin antibody. In one embodiment of the invention, the donor and acceptor antibodies are monoclonal antibodies from different species. For example, the acceptor antibody is a human antibody (to minimize its antigenicity in a human), in which case the resulting CDR-grafted antibody is termed a “humanized” antibody.
Recombinant anti-gelsolin antibodies, such as chimeric and humanized monoclonal antibodies, comprising both human and non-human portions, can be made using standard recombinant DNA techniques, and are within the scope of the invention. For some uses, including in vivo use of the binding agent of the invention in humans as well as use of these agents in vitro detection assays, it is preferable to use chimeric, humanized, or human anti-gelsolin antibodies. Such chimeric and humanized monoclonal antibodies can be produced by recombinant DNA techniques known in the art. Such useful methods include, e.g., but are not limited to, methods described in International Application No. PCT/US86/02269; U.S. Pat. No. 5,225,539; European Patent No. 184187, European Patent No. 171496; European Patent No. 173494; PCT International Publication No. WO 86/01533; U.S. Pat. Nos. 4,816,567; 5,225,539; European Patent No. 125023; Better, et al., Science 240:1041-1043 (1988); Liu, et al., Proc. Natl. Acad. Sci. USA 84:3439-3443 (1987); Liu, et al., J. Immunol. 139:3521-3526 (1987); Sun, et al., Proc. Natl. Acad. Sci. USA 84:214-218 (1987); Nishimura, et al., Cancer Res. 47:999-1005 91987); Wood, et al., Nature 314:446-449 (1985); Shaw, et al., J. Natl. Cancer Inst. 80:1553-1559 (1988); Morrison, Science 229:1202-1207 (1985); Oi, et al., BioTechniques 4:214 (1986); Jones, et al., Nature 321:552-525 (1986); Verhoeyan, et al., Science 239:1534 1988); Morrison, Science 229:1202 (1985); Oi et al., BioTechniques 4:214 (1986); Gillies et al., J. Immunol. Methods, 125:191-202 (1989); U.S. Pat. No. 5,807,715; and Beidler, et al., J. Immunol. 141:4053-4060 (1988). For example, antibodies can be humanized using a variety of techniques including CDR-grafting (EP 0 239 400; WO 91/09967; U.S. Pat. Nos. 5,530,101; 5,585,089; 5,859,205; 6,248,516; EP460167), veneering or resurfacing (EP 0 592 106; EP 0 519 596; Padlan E. A., Molecular Immunology, 28:489-498 (1991); Studnicka et al., Protein Engineering 7:805-814 (1994); Roguska et al., PNAS 91:969-973 (1994)), and chain shuffling (U.S. Pat. No. 5,565,332). In one embodiment, a cDNA encoding a murine anti-gelsolin monoclonal antibody is digested with a restriction enzyme selected specifically to remove the sequence encoding the Fc constant region, and the equivalent portion of a cDNA encoding a human Fc constant region is substituted (see Robinson et al., PCT/US86/02269; Akira et al., European Patent Application 184,187; Taniguchi, European Patent Application 171,496; Morrison et al., European Patent Application 173,494; Neuberger et al., WO 86/01533; Cabilly et al. U.S. Pat. No. 4,816,567; Cabilly et al., European Patent Application 125,023; Better et al., Science 240:1041-1043 (1988); Liu et al., Proc. Natl. Acad. Sci. USA 84:3439-3443 (1987); Liu et al., J Immunol 139:3521-3526 (1987); Sun et al., Proc. Natl. Acad. Sci. USA 84:214-218 (1987); Nishimura et al., Cancer Res 47:999-1005 (1987); Wood et al., Nature 314:446-449 (1985); and Shaw et al., J. Natl. Cancer Inst. 80:1553-1559 (1988); U.S. Pat. No. 6,180,370; U.S. Pat. Nos. 6,300,064; 6,696,248; 6,706,484; 6,828,422.
In one embodiment, the present invention allows the construction of humanized anti-gelsolin antibodies that are unlikely to induce a human anti-mouse antibody (hereinafter referred to as “HAMA”) response, while still having an effective antibody effector function. As used herein, the terms “human” and “humanized”, in relation to antibodies, relate to any antibody which is expected to elicit a therapeutically tolerable weak immunogenic response in a human subject. In one embodiment, the present invention provides for a humanized gelsolin antibodies, heavy and light chain immunoglobulins.
CDR Antibodies.
In one embodiment, the binding agent of the invention is an anti-gelsolin CDR antibody. Generally the donor and acceptor antibodies used to generate the anti-gelsolin CDR antibody are monoclonal antibodies from different species; typically the acceptor antibody is a human antibody (to minimize its antigenicity in a human), in which case the resulting CDR-grafted antibody is termed a “humanized” antibody. The graft may be of a single CDR (or even a portion of a single CDR) within a single VH or VL of the acceptor antibody, or can be of multiple CDRs (or portions thereof) within one or both of the VH and VL. Frequently all three CDRs in all variable domains of the acceptor antibody will be replaced with the corresponding donor CDRs, though one need replace only as many as necessary to permit adequate binding of the resulting CDR-grafted antibody to MetAp3. Methods for generating CDR-grafted and humanized antibodies are taught by Queen et al. U.S. Pat. No. 5,585,089, U.S. Pat. No. 5,693,761; U.S. Pat. No. 5,693,762; and Winter U.S. Pat. No. 5,225,539; and EP 0682040. Methods useful to prepare VH and VL polypeptides are taught by Winter et al., U.S. Pat. Nos. 4,816,397; 6,291,158; 6,291,159; 6,291,161; 6,545,142; EP 0368684; EPO451216; EP0120694.
After selecting suitable framework region candidates from the same family and/or the same family member, either or both the heavy and light chain variable regions are produced by grafting the CDRs from the originating species into the hybrid framework regions. Assembly of hybrid antibodies or hybrid antibody fragments having hybrid variable chain regions with regard to either of the above aspects can be accomplished using conventional methods known to those skilled in the art. For example, DNA sequences encoding the hybrid variable domains described herein (i.e., frameworks based on the target species and CDRs from the originating species) can be produced by oligonucleotide synthesis and/or PCR. The nucleic acid encoding CDR regions can also be isolated from the originating species antibodies using suitable restriction enzymes and ligated into the target species framework by ligating with suitable ligation enzymes. Alternatively, the framework regions of the variable chains of the originating species antibody can be changed by site-directed mutagenesis.
Since the hybrids are constructed from choices among multiple candidates corresponding to each framework region, there exist many combinations of sequences which are amenable to construction in accordance with the principles described herein. Accordingly, libraries of hybrids can be assembled having members with different combinations of individual framework regions. Such libraries can be electronic database collections of sequences or physical collections of hybrids.
This process typically does not alter the acceptor antibody's FRs flanking the grafted CDRs. However, one skilled in the art can sometimes improve antigen binding affinity of the resulting anti-gelsolin CDR grafted antibody by replacing certain residues of a given FR to make the FR more similar to the corresponding FR of the donor antibody. Preferred locations of the substitutions include amino acid residues adjacent to the CDR, or which are capable of interacting with a CDR. (See, e.g., U.S. Pat. No. 5,585,089, especially columns 12-16). Or one skilled in the art can start with the donor FR and modify it to be more similar to the acceptor FR or a human consensus FR. Techniques for making these modifications are known in the art. Particularly if the resulting FR fits a human consensus FR for that position, or is at least 90% or more identical to such a consensus FR, doing so may not increase the antigenicity of the resulting modified anti-gelsolin CDR antibody significantly compared to the same antibody with a fully human FR.
Fusion Proteins.
In one embodiment, the binding agent of the invention is a fusion protein. The gelsolin binding agents of the present invention, when fused to a second protein, can be used as an antigenic tag. Examples of domains that can be fused to polypeptides include not only heterologous signal sequences, but also other heterologous functional regions. The fusion does not necessarily need to be direct, but can occur through linker sequences. Moreover, fusion proteins of the present invention can also be engineered to improve characteristics of the gelsolin binding agent. For instance, a region of additional amino acids, particularly charged amino acids, can be added to the N-terminus of the gelsolin binding agent to improve stability and persistence during purification from the host cell or subsequent handling and storage. Also, peptide moieties can be added to the gelsolin binding agent to facilitate purification. Such regions can be removed prior to final preparation of the gelsolin binding agent. The addition of peptide moieties to facilitate handling of polypeptides are familiar and routine techniques in the art. The gelsolin binding agent of the invention can be fused to marker sequences, such as a peptide which facilitates purification of the fused polypeptide. In preferred embodiments, the marker amino acid sequence is a hexa-histidine peptide, such as the tag provided in a pQE vector (QIAGEN, Inc., Chatsworth, Calif.), among others, many of which are commercially available. As described in Gentz et al., Proc. Natl. Acad. Sci. USA 86:821-824 (1989), for instance, hexa-histidine provides for convenient purification of the fusion protein. Another peptide tag useful for purification, the “HA” tag, corresponds to an epitope derived from the influenza hemagglutinin protein. Wilson et al., Cell 37:767 (1984).
Thus, any of these above fusions can be engineered using the polynucleotides or the polypeptides of the present invention. Also, the fusion protein can show an increased half-life in vivo.
Fusion proteins having disulfide-linked dimeric structures (due to the IgG) can be more efficient in binding and neutralizing other molecules, than the monomeric secreted protein or protein fragment alone. Fountoulakis et al., J. Biochem. 270:3958-3964 (1995).
Similarly, EP-A-0 464 533 discloses fusion proteins comprising various portions of constant region of immunoglobulin molecules together with another human protein or part thereof. In many cases, the Fc part in a fusion protein is beneficial in therapy and diagnosis, and thus can result in, e.g., improved pharmacokinetic properties. See EP-A 0232 262. Alternatively, deleting the Fc part after the fusion protein has been expressed, detected, and purified, would be desired. For example, the Fc portion can hinder therapy and diagnosis if the fusion protein is used as an antigen for immunizations. In drug discovery, e.g., human proteins, such as hIL-5, have been fused with Fc portions for the purpose of high-throughput screening assays to identify antagonists of hIL-5. Bennett et al., J. Molecular Recognition 8:52-58 (1995); Johanson et al., J. Biol. Chem., 270:9459-9471 (1995).
Labeled Gelsolin-Binding Agent.
In one embodiment, the gelsolin binding agent of the present invention is coupled with a label moiety, i.e., detectable group. The particular label or detectable group conjugated to the gelsolin binding agent of the invention is not a critical aspect of the invention, so long as it does not significantly interfere with the specific binding of the gelsolin binding agent of the present invention to the gelsolin polypeptide or the gelsolin-like polypeptide. The detectable group can be any material having a detectable physical or chemical property. Such detectable labels have been well-developed in the field of immunoassays and imaging, in general, most any label useful in such methods can be applied to the present invention. Thus, a label is any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical or chemical means. Useful labels in the present invention include magnetic beads (e.g., Dynabeads™) fluorescent dyes (e.g., fluorescein isothiocyanate, Texas red, rhodamine, and the like), radiolabels (e.g., 3H, 14C, 35S, 125I, 121I, 131I, 112In, 99mTc), other imaging agents such as microbubbles (for ultrasound imaging), 18F, 11C, 15O, (for Positron emission tomography), 99mTC, 111In (for Single photon emission tomography), enzymes (e.g., horse radish peroxidase, alkaline phosphatase and others commonly used in an ELISA), and calorimetric labels such as colloidal gold or colored glass or plastic (e.g., polystyrene, polypropylene, latex, and the like) beads. Patents that described the use of such labels include U.S. Pat. Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345; 4,277,437; 4,275,149; and 4,366,241, each incorporated herein by reference in their entirety and for all purposes. See also Handbook of Fluorescent Probes and Research Chemicals (6th Ed., Molecular Probes, Inc., Eugene Oreg. (1996)).
The label can be coupled directly or indirectly to the desired component of an assay according to methods well known in the art. As indicated above, a wide variety of labels can be used, with the choice of label depending on sensitivity required, ease of conjugation with the compound, stability requirements, available instrumentation, and disposal provisions.
Non-radioactive labels are often attached by indirect means. Generally, a ligand molecule (e.g., biotin) is covalently bound to the molecule. The ligand then binds to an anti-ligand (e.g., streptavidin) molecule which is either inherently detectable or covalently bound to a signal system, such as a detectable enzyme, a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands can be used. Where a ligand has a natural anti-ligand, e.g., biotin, thyroxine, and cortisol, it can be used in conjunction with the labeled, naturally-occurring anti-ligands. Alternatively, any haptenic or antigenic compound can be used in combination with an antibody, e.g., an anti-gelsolin antibody.
The molecules can also be conjugated directly to signal generating compounds, e.g., by conjugation with an enzyme or fluorophore. Enzymes of interest as labels will primarily be hydrolases, particularly phosphatases, esterases and glycosidases, or oxidoreductases, particularly peroxidases. Fluorescent compounds useful as labeling moieties, include, but are not limited to, e.g., fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, and the like. Chemiluminescent compounds useful as labelling moieties, include, but are not limited to, e.g., luciferin, and 2,3-dihydrophthalazinediones, e.g., luminol. For a review of various labeling or signal-producing systems which can be used, see, U.S. Pat. No. 4,391,904.
Means of detecting labels are well known to those of skill in the art. Thus, for example, where the label is a radioactive label, means for detection include a scintillation counter or photographic film as in autoradiography. Where the label is a fluorescent label, it can be detected by exciting the fluorochrome with the appropriate wavelength of light and detecting the resulting fluorescence. The fluorescence can be detected visually, by means of photographic film, by the use of electronic detectors such as charge coupled devices (CCDs) or photomultipliers and the like. Similarly, enzymatic labels can be detected by providing the appropriate substrates for the enzyme and detecting the resulting reaction product. Finally simple colorimetric labels can be detected simply by observing the color associated with the label. Thus, in various dipstick assays, conjugated gold often appears pink, while various conjugated beads appear the color of the bead.
Some assay formats do not require the use of labeled components. For instance, agglutination assays can be used to detect the presence of the target antibodies, e.g., the anti-gelsolin antibodies. In this case, antigen-coated particles are agglutinated by samples comprising the target antibodies. In this format, none of the components need be labeled and the presence of the target antibody is detected by simple visual inspection.
C. Identifying and Characterizing the Gelsolin-Binding Agents of the Invention
Methods for Identifying and/or Screening the Binding Agents of the Invention.
Methods useful to identify and screen the binding agents, e.g., anti-gelsolin antibodies and anti-gelsolin antibody-related polypeptides, that possess the desired specificity to the gelsolin polypeptide include any immunologically-mediated techniques known within the art. Components of an immune response can be detected in vitro by various methods that are well known to those of ordinary skill in the art. For example, (1) cytotoxic T lymphocytes can be incubated with radioactively labeled target cells and the lysis of these target cells detected by the release of radioactivity; (2) helper T lymphocytes can be incubated with antigens and antigen presenting cells and the synthesis and secretion of cytokines measured by standard methods (Windhagen A; et al., Immunity, 2:373-80 (1995)); (3) antigen presenting cells can be incubated with whole protein antigen and the presentation of that antigen on MHC detected by either T lymphocyte activation assays or biophysical methods (Harding et al., Proc. Natl. Acad. Sci., 86:4230-4 (1989)); (4) mast cells can be incubated with reagents that cross-link their Fc-epsilon receptors and histamine release measured by enzyme immunoassay (Siraganian et al., TIPS, 4:432-437 (1983)); and (5) enzyme-linked immunosorbent assay (ELISA).
Similarly, products of an immune response in either a model organism (e.g., mouse) or a human subject can also be detected by various methods that are well known to those of ordinary skill in the art. For example, (1) the production of antibodies in response to vaccination can be readily detected by standard methods currently used in clinical laboratories, e.g., an ELISA; (2) the migration of immune cells to sites of inflammation can be detected by scratching the surface of skin and placing a sterile container to capture the migrating cells over scratch site (Peters et al., Blood, 72:1310-5 (1988)); (3) the proliferation of peripheral blood mononuclear cells in response to mitogens or mixed lymphocyte reaction can be measured using 3H-thymidine; (4) the phagocytic capacity of granulocytes, macrophages, and other phagocytes in PBMCs can be measured by placing PMBCs in wells together with labeled particles (Peters et al., Blood, 72:1310-5 (1988)); and (5) the differentiation of immune system cells can be measured by labeling PBMCs with antibodies to CD molecules such as CD4 and CD8 and measuring the fraction of the PBMCs expressing these markers.
In one embodiment, gelsolin binding agents of the invention are selected using display of candidate binding agents on the surface of replicable genetic packages. See, e.g., U.S. Pat. Nos. 5,514,548; 5,837,500; 5,871,907; 5,885,793; 5,969,108; 6,225,447; 6,291,650; 6,492,160; EP 585 287; EP 605522; EP 616640; EP 1024191; EP 589 877; EP 774 511; EP 844 306. Methods useful for producing/selecting a filamentous bacteriophage particle containing a phagemid genome encoding for a binding molecule with a desired specificity has been described. See, e.g., EP 774 511; U.S. Pat. No. 5,871,907; U.S. Pat. No. 5,969,108; U.S. Pat. No. 6,225,447; U.S. Pat. No. 6,291,650; U.S. Pat. No. 6,492,160.
In one embodiment, gelsolin binding agents of the invention are selected using display of candidate binding agents on the surface of a yeast host cell. Methods useful for the isolation of scFv polypeptides by yeast surface display have been described by Kieke et al., Protein Eng., 10(11):1303-10 (1997).
In one embodiment, gelsolin binding agents of the invention are selected using ribosome display. Methods useful for identifying ligands in peptide libraries using ribosome display have been described by Mattheakis et al., Proc. Natl. Acad. Sci. USA 91:9022-26 (1994); and Hanes et al., Proc. Natl. Acad. Sci. USA 94:4937-42 (1997).
In one embodiment, gelsolin binding agents of the invention are selected using tRNA display of candidate binding agents. Methods useful for in vitro selection of ligands using tRNA display have been described by Merryman et al., Chem. Biol. 9:741-46 (2002).
In one embodiment, gelsolin binding agents of the invention are selected using RNA display. Methods useful for selecting peptides and proteins using RNA display libraries have been described by Roberts et al. Proc. Natl. Acad. Sci. USA, 94:12297-302 (1997); and Nemoto et al., FEBS Lett., 414:405-8 (1997). Methods useful for selecting peptides and proteins using unnatural RNA display libraries have been described by Frankel et al., Curr. Opin. Struct. Biol., 13:506-12 (2003).
In one embodiment, gelsolin binding agents of the invention are expressed in the periplasm of gram negative bacteria and mixed with labeled gelsolin polypeptide. See WO 02/34886. In clones expressing recombinant polypeptides with affinity for the gelsolin polypeptide, the concentration of the labeled gelsolin polypeptide bound to the binding agents is increased and allows the cells to be isolated from the rest of the library as described in Harvey et al., Proc. Natl. Acad. Sci. 22:9193-98 (2004) and U.S. Pat. Publication No. 2004/0058403.
After selection of the desired gelsolin binding agent, it is contemplated that it can be produced in large volume by any technique known to those skilled in the art, e.g., prokaryotic or eukaryotic cell expression and the like. The gelsolin binding agents which are, e.g., but not limited to, anti-gelsolin hybrid antibodies or fragments can be produced by using conventional techniques to construct an expression vector that encodes an antibody heavy chain in which the CDRs and, if necessary, a minimal portion of the variable region framework, that are required to retain original species antibody binding specificity (as engineered according to the techniques described herein) are derived from the originating species antibody and the remainder of the antibody is derived from a target species immunoglobulin which can be manipulated as described herein, thereby producing a vector for the expression of a hybrid antibody heavy chain.
Measurement of Gelsolin Binding.
In one embodiment, a gelsolin binding assay refers to an assay format wherein a gelsolin polypeptide and a gelsolin binding agent are mixed under conditions suitable for binding between the gelsolin or gelsolin-like polypeptide and the gelsolin binding agent and assessing the amount of binding between the gelsolin or gelsolin-like polypeptide and the gelsolin binding agent. The amount of binding is compared with a suitable control, which can be the amount of binding in the absence of the gelsolin polypeptide, the amount of the binding in the presence of non-specific immunoglobulin composition, or both. The amount of binding can be assessed by any suitable method. Binding assay methods include, e.g., ELISA, radioimmunoassays, scintillation proximity assays, fluorescence energy transfer assays, liquid chromatography, membrane filtration assays, and the like. Biophysical assays for the direct measurement of gelsolin polypeptide binding to gelsolin binding agents are, e.g., nuclear magnetic resonance, fluorescence, fluorescence polarization, surface plasmon resonance (BIACOR chips) and the like. Specific binding is determined by standard assays known in the art, e.g., radioligand binding assays, ELISA, FRET, immunoprecipitation, SPR, NMR (2D-NMR), mass spectroscopy and the like. If the specific binding of a candidate gelsolin binding agent is at least 1 percent greater than the binding observed in the absence of the candidate gelsolin binding agent, the candidate gelsolin binding agent is useful as a gelsolin binding agent of the invention.
Co-crystals of the gelsolin polypeptides and the gelsolin binding agents are also provided by the present invention as a method of determining molecular interactions. Conditions suitable for binding between the gelsolin binding agent and a gelsolin polypeptide will depend on the compound and its ligand and can be readily determined by one of ordinary skill in the art.
General. The binding agents of the invention are useful in methods known in the art relating to the localization and/or quantitation of a gelsolin or gelsolin-like polypeptide (e.g., for use in measuring levels of the gelsolin or gelsolin-like polypeptide within appropriate physiological samples, for use in diagnostic methods, for use in imaging the polypeptide, and the like). Binding agents of the invention are useful to isolate a gelsolin polypeptide by standard techniques, such as affinity chromatography or immunoprecipitation. A gelsolin binding agent of the invention can facilitate the purification of natural immunoreactive gelsolin polypeptides or gelsolin-like polypeptides from biological samples, e.g., mammalian sera, urine, or cells as well as recombinantly-produced immunoreactive gelsolin polypeptides or gelsolin-like polypeptides expressed in a host system. Moreover, gelsolin binding agent can be used to detect an immunoreactive gelsolin polypeptide or a gelsolin-like polypeptide (e.g., in plasma, urine, a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the immunoreactive polypeptide. The gelsolin binding agents of the invention can be used diagnostically to monitor immunoreactive gelsolin and/or gelsolin-like polypeptide levels in tissue as part of a clinical testing procedure, e.g., to determine the efficacy of a given treatment regimen. As noted above, the detection can be facilitated by coupling (i.e., physically linking) the gelsolin binding agent of the invention to a detectable substance.
Detection of Gelsolin Polypeptide.
An exemplary method for detecting the presence or absence of a gelsolin polypeptide or a gelsolin-like polypeptide in a biological sample involves obtaining a biological sample from a test subject and contacting the biological sample with a gelsolin binding agent of the invention capable of detecting a gelsolin polypeptide or a gelsolin-like polypeptide such that the presence of a gelsolin polypeptide or a gelsolin-like polypeptide is detected in the biological sample. An example of a gelsolin binding agent is an antibody raised against SEQ ID NO.: 1 or a homlog or fragment thereof, capable of binding to a gelsolin polypeptide (a gelsolin polypeptide fragment) or a gelsolin-like polypeptide. The gelsolin binding agent may be labeled. The term “labeled,” with regard to the binding agent is intended to encompass direct labeling of the binding agent by coupling (i.e., physically linking) a detectable substance to the binding agent, as well as indirect labeling of the binding agent by reactivity with another compound that is directly labeled. Examples of indirect labeling include detection of a primary antibody using a fluorescently-labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with fluorescently-labeled streptavidin.
In suitable embodiments, a urine sample is assayed for a gelsolin polypeptide or gelsolin-like polypeptide. While not wishing to be limited by theory, urine gelsolin or gelsolin-like polypeptides may be a product of the utilization of plasma gelsolin. Plasma gelsolin is a 90 kDa polypeptide and has a critical biological function in binding and clearance of actin and other toxic substances released from injured cells. Lower levels of plasma gelsolin are associated with many diseases, suggesting that depletion of plasma gelsolin occurs during the disease process. After binding to actin, full-length gelsolin may be cleared by the kidney. Thus, urine gelsolin fragments represent a biomarker for in vivo utilization of plasma gelsolin.
The detection method of the invention can be used to detect a gelsolin polypeptide or a gelsolin-like polypeptide in a biological sample in vitro as well as in vivo. In vitro techniques for detection of a gelsolin polypeptide or a gelsolin-like polypeptide include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations, radioimmunoassay, and immunofluorescence. Furthermore, in vivo techniques for detection of a gelsolin polypeptide or a gelsolin-like polypeptide include introducing into a subject a labeled gelsolin binding agent, e.g., an anti-gelsolin antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. In one embodiment, the biological sample contains polypeptide molecules from the test subject.
Immunoassay and Imaging.
A gelsolin binding agent of the present invention can be used to assay gelsolin polypeptide levels or gelsolin-like polypeptide levels in a biological sample (e.g., human plasma) using antibody-based techniques. For example, protein expression in tissues can be studied with classical immunohistological methods. Jalkanen, M. et al., J. Cell. Biol. 101:976-985 (1985); Jalkanen, M. et al., J. Cell. Biol. 105:3087-3096 (1987. Other antibody-based methods useful for detecting protein gene expression include immunoassays, such as the enzyme linked immunosorbent assay (ELISA) and the radioimmunoassay (RIA). Suitable antibody assay labels are known in the art and include enzyme labels, such as, glucose oxidase, and radioisotopes or other radioactive agent, such as iodine (121I, 121I, 131I) carbon (14C), sulfur (35S), tritium (3H), indium (112In), and technetium (99mTc), and fluorescent labels, such as fluorescein and rhodamine, and biotin.
In addition to assaying gelsolin polypeptide levels or gelsolin-like polypeptide levels in a biological sample, gelsolin polypeptide levels or gelsolin-like polypeptide levels can also be detected in vivo by imaging. A gelsolin binding agent, e.g., an anti-gelsolin antibody labels or markers for in vivo imaging of the gelsolin polypeptide levels or the gelsolin-like polypeptide include those detectable by X-radiography, NMR or ESR. For X-radiography, suitable labels include radioisotopes such as barium or cesium, which emit detectable radiation but are not overtly harmful to the subject. Suitable markers for NMR and ESR include those with a detectable characteristic spin, such as deuterium, which can be incorporated into the gelsolin binding agent by labeling of nutrients for the relevant scFv clone.
A gelsolin binding agent which has been labeled with an appropriate detectable imaging moiety, such as a radioisotope (e.g., 131I, 112In, 99mTc), a radio-opaque substance, or a material detectable by nuclear magnetic resonance, is introduced (e.g., parenterally, subcutaneously, or intraperitoneally) into the subject. It will be understood in the art that the size of the subject and the imaging system used will determine the quantity of imaging moiety needed to produce diagnostic images. In the case of a radioisotope moiety, for a human subject, the quantity of radioactivity injected will normally range from about 5 to 20 millicuries of 99mTc. The labeled gelsolin binding agent will then preferentially accumulate at the location of cells which contain the specific target polypeptide. For example, in vivo tumor imaging is described in S. W. Burchiel et al., Tumor Imaging: The Radiochemical Detection of Cancer 13 (1982).
Thus, the invention provides a diagnostic method of a medical condition, which involves: (a) assaying the expression of a polypeptide by measuring binding of a gelsolin binding agent of the present invention in cells or body fluid of an individual; (b) comparing the amount of protein with a standard, whereby an increase or decrease in the assayed polypeptide compared to the standard level is indicative of a medical condition.
Affinity Purification.
The gelsolin binding agents of the present invention may be used to purify immunoreacitve gelsolin (e.g., native plasma gelsolin) from a sample. In some embodiments, antibodies (e.g., GN3E9, GC1C10, GC5D1, GC4A10, and/or GF2D6) may be immobilized on a solid support. Examples of such solid supports include plastics such as polycarbonate, complex carbohydrates such as agarose and sepharose, acrylic resins and such as polyacrylamide and latex beads. Techniques for coupling antibodies to such solid supports are well known in the art. (Weir et al., “Handbook of Experimental Immunology” 4th Ed., Blackwell Scientific Publications, Oxford, England, Chapter 10 (1986); Jacoby et al., Meth. Enzym. 34 Academic Press, N.Y. (1974).)
The simplest method to bind the antigen to the antibody-support matrix is to collect the beads in a column and pass the antigen solution down the column. The efficiency of this method depends on the contact time between the immobilized antibody and the antigen, which can be extended by using low flow rates. The immobilized antibody captures the antigen as it flows past. Alternatively, an antigen can be contacted with the antibody-support matrix by mixing the antigen solution with the support (e.g., beads) and rotating or rocking the slurry, allowing maximum contact between the antigen and the immobilized antibody. After the binding reaction has been completed, the slurry is passed into a column for collection of the beads. The beads are washed using a suitable washing buffer and then the pure or substantially pure antigen is eluted.
An antibody or polypeptide of interest can be conjugated to a solid support, such as a bead. In addition, a first solid support such as a bead can also be conjugated, if desired, to a second solid support, which can be a second bead or other support, by any suitable means, including those disclosed herein for conjugation of a polypeptide to a support. Accordingly, any of the conjugation methods and means disclosed herein with reference to conjugation of a polypeptide to a solid support can also be applied for conjugation of a first support to a second support, where the first and second solid support can be the same or different.
Appropriate linkers, which can be cross-linking agents, for use for conjugating a polypeptide to a solid support include a variety of agents that can react with a functional group present on a surface of the support, or with the polypeptide, or both. Reagents useful as cross-linking agents include homo-bi-functional and, in particular, hetero-bi-functional reagents. Useful bi-functional cross-linking agents include, but are not limited to, N-SIAB, dimaleimide, DTNB, N-SATA, N-SPDP, SMCC and 6-HYNIC. A cross-linking agent can be selected to provide a selectively cleavable bond between a polypeptide and the solid support. For example, a photolabile cross-linker, such as 3-amino-(2-nitrophenyl)propionic acid can be employed as a means for cleaving a polypeptide from a solid support. (Brown et al., Mol. Divers. 4-12 (1995); Rothschild et al., Nucl. Acids Res. 24:351-66 (1996); and U.S. Pat. No. 5,643,722). Other cross-linking reagents are well-known in the art. (See, e.g., Wong (1991), supra; and Hermanson (1996), supra).
An antibody or polypeptide can be immobilized on a solid support, such as a bead, through a covalent amide bond formed between a carboxyl group functionalized bead and the amino terminus of the polypeptide or, conversely, through a covalent amide bond formed between an amino group functionalized bead and the carboxyl terminus of the polypeptide. In addition, a bi-functional trityl linker can be attached to the support, e.g, to the 4-nitrophenyl active ester on a resin, such as a Wang resin, through an amino group or a carboxyl group on the resin via an amino resin. Using a bi-functional trityl approach, the solid support can require treatment with a volatile acid, such as formic acid or trifluoracetic acid to ensure that the polypeptide is cleaved and can be removed. In such a case, the polypeptide can be deposited as a beadless patch at the bottom of a well of a solid support or on the flat surface of a solid support. After addition of a matrix solution, the polypeptide can be desorbed into a MS.
Hydrophobic trityl linkers can also be exploited as acid-labile linkers by using a volatile acid or an appropriate matrix solution, e.g., a matrix solution containing 3-HPA, to cleave an amino linked trityl group from the polypeptide. Acid lability can also be changed. For example, trityl, monomethoxytrityl, dimethoxytrityl or trimethoxytrityl can be changed to the appropriate p-substituted, or more acid-labile tritylamine derivatives, of the polypeptide, i.e., trityl ether and tritylamine bonds can be made to the polypeptide. Accordingly, a polypeptide can be removed from a hydrophobic linker, e.g., by disrupting the hydrophobic attraction or by cleaving tritylether or tritylamine bonds under acidic conditions, including, if desired, under typical MS conditions, where a matrix, such as 3-HPA acts as an acid.
Orthogonally cleavable linkers can also be useful for binding a first solid support, e.g., a bead to a second solid support, or for binding a polypeptide of interest to a solid support. Using such linkers, a first solid support, e.g., a bead, can be selectively cleaved from a second solid support, without cleaving the polypeptide from the support; the polypeptide then can be cleaved from the bead at a later time. For example, a disulfide linker, which can be cleaved using a reducing agent, such as DTT, can be employed to bind a bead to a second solid support, and an acid cleavable bi-functional trityl group could be used to immobilize a polypeptide to the support. As desired, the linkage of the polypeptide to the solid support can be cleaved first, e.g., leaving the linkage between the first and second support intact. Trityl linkers can provide a covalent or hydrophobic conjugation and, regardless of the nature of the conjugation, the trityl group is readily cleaved in acidic conditions.
For example, a bead can be bound to a second support through a linking group which can be selected to have a length and a chemical nature such that high density binding of the beads to the solid support, or high density binding of the polypeptides to the beads, is promoted. Such a linking group can have, e.g., “tree-like” structure, thereby providing a multiplicity of functional groups per attachment site on a solid support. Examples of such linking group; include polylysine, polyglutamic acid, penta-erythrole and tris-hydroxy-aminomethane.
Noncovalent Binding Association.
An antibody or polypeptide can be conjugated to a solid support, or a first solid support can also be conjugated to a second solid support, through a noncovalent interaction. For example, a magnetic bead made of a ferromagnetic material, which is capable of being magnetized, can be attracted to a magnetic solid support, and can be released from the support by removal of the magnetic field. Alternatively, the solid support can be provided with an ionic or hydrophobic moiety, which can allow the interaction of an ionic or hydrophobic moiety, respectively, with a polypeptide, e.g., a polypeptide containing an attached trityl group or with a second solid support having hydrophobic character.
A solid support can also be provided with a member of a specific binding pair and, therefore, can be conjugated to a polypeptide or a second solid support containing a complementary binding moiety. For example, a bead coated with avidin or with streptavidin can be bound to a polypeptide having a biotin moiety incorporated therein, or to a second solid support coated with biotin or derivative of biotin, such as imino-biotin.
It should be recognized that any of the binding members disclosed herein or otherwise known in the art can be reversed. Thus, biotin, e.g., can be incorporated into either a polypeptide or a solid support and, conversely, avidin or other biotin binding moiety would be incorporated into the support or the polypeptide, respectively. Other specific binding pairs contemplated for use herein include, but are not limited to, hormones and their receptors, enzyme, and their substrates, a nucleotide sequence and its complementary sequence, an antibody and the antigen to which it interacts specifically, and other such pairs knows to those skilled in the art.
A. Diagnostic Uses of Gelsolin Binding Agents
General.
The gelsolin binding compositions of the invention are useful in diagnostic methods. As such, the present invention provides methods using the binding agents of the invention in the diagnosis of gelsolin-related medical conditions in a subject. Binding agents of the invention may be selected such that they have any level of epitope binding specificity and very high binding affinity to the gelsolin polypeptide. In general, the higher the binding affinity of an binding agent the more stringent wash conditions can be performed in an immunoassay to remove nonspecifically bound material without removing target polypeptide. Accordingly, gelsolin binding agents of the invention may in diagnostic assays usually have binding affinities of at least 108, 109, 1010, 1011 or 1012 M−1. Further, it is desirable that gelsolin binding agents used as diagnostic reagents have a sufficient kinetic on-rate to reach equilibrium under standard conditions in at least 12 h, preferably at least five (5) h and more preferably at least one (1) hour.
Although gelsolin binding agents can be used as diagnostic reagents for any kind of sample, they are most useful as diagnostic reagents for human biological samples. Gelsolin binding agents can be used to detect a given gelsolin or gelsolin-like polypeptide in a variety of standard assay formats. Such formats include immunoprecipitation, Western blotting, ELISA, radioimmunoassay, and immunometric assays. See Harlow & Lane, Antibodies, A Laboratory Manual (Cold Spring Harbor Publications, New York (1988)); U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,879,262; 4,034,074, 3,791,932; 3,817,837; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; and 4,098,876. Biological samples can be obtained from any tissue or body fluid of a subject.
Immunometric or sandwich assays are a preferred format for the diagnostic methods of the present invention. See U.S. Pat. Nos. 4,376,110, 4,486,530, 5,914,241, and 5,965,375. Such assays use one gelsolin binding agent, e.g., an anti-gelsolin antibody or a population of anti-gelsolin antibodies immobilized to a solid phase, and another anti-gelsolin antibody or a population of anti-gelsolin antibodies in solution. Typically, the solution anti-gelsolin antibody or population of anti-gelsolin antibodies is labeled. If an antibody population is used, the population can contain antibodies binding to different epitope specificities within the target polypeptide. Accordingly, the same population can be used for both solid phase and solution antibody. If anti-gelsolin monoclonal antibodies are used, first and second gelsolin monoclonal antibodies having different binding specificities are used for the solid and solution phase. Solid phase (also referred to as “capture”) and solution (also referred to as “detection”) antibodies can be contacted with target antigen in either order or simultaneously. If the solid phase antibody is contacted first, the assay is referred to as being a forward assay. Conversely, if the solution antibody is contacted first, the assay is referred to as being a reverse assay. If the target is contacted with both antibodies simultaneously, the assay is referred to as a simultaneous assay. After contacting the gelsolin polypeptide with the anti-gelsolin antibody, a sample is incubated for a period that usually varies from about 10 min to about 24 hr and is usually about 1 hr. A wash step is then performed to remove components of the sample not specifically bound to the anti-gelsolin antibody being used as a diagnostic reagent. When solid phase and solution antibodies are bound in separate steps, a wash can be performed after either or both binding steps. After washing, binding is quantified, typically by detecting a label linked to the solid phase through binding of labeled solution antibody. Usually for a given pair of antibodies or populations of antibodies and given reaction conditions, a calibration curve is prepared from samples containing known concentrations of target antigen. Concentrations of the gelsolin polypeptide in samples being tested are then read by interpolation from the calibration curve. Analyte can be measured either from the amount of labeled solution antibody bound at equilibrium or by kinetic measurements of bound labeled solution antibody at a series of time points before equilibrium is reached. The slope of such a curve is a measure of the concentration of the gelsolin polypeptide in a sample
Suitable supports for use in the above methods include, e.g., nitrocellulose membranes, nylon membranes, and derivatized nylon membranes, and also particles, such as agarose, a dextran-based gel, dipsticks, particulates, microspheres, magnetic particles, test tubes, microtiter wells, SEPHADEX™ (Amersham Pharmacia Biotech, Piscataway N.J.), and the like. Immobilization can be by absorption or by covalent attachment. Optionally, anti-gelsolin antibodies can be joined to a linker molecule, such as biotin for attachment to a surface bound linker, such as avidin.
The invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with gelsolin polypeptide expression or activity. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with a gelsolin polypeptide. Furthermore, the methods of the present invention can also be used to assess whether an individual expresses a gelsolin polypeptide or a polymorphic form of the gelsolin polypeptide in instances where a gelsolin binding agent of the present invention has greater affinity for the gelsolin polypeptide for its polymorphic form (or vice versa).
The binding of a gelsolin binding agent of the invention to a gelsolin polypeptide or a gelsolin-like polypeptide, e.g., can be utilized to identify a subject having or at risk of developing a disorder associated with altered gelsolin polypeptide or gelsolin-like polypeptide levels. Alternatively, the prognostic assays can be utilized to identify a subject having or at risk for developing the disease or disorder. Thus, the invention provides a method for identifying a disease or condition associated with an aberrant gelsolin polypeptide or gelsolin-like polypeptide expression or activity in which a test sample is obtained from a subject and the gelsolin or gelsolin-like polypeptide detected, wherein the presence of an alteration of gelsolin or gelsolin-like polypeptide is diagnostic for a subject having or at risk of developing a disease or condition associated with an aberrant gelsolin polypeptide or gelsolin-like polypeptide expression or activity.
Furthermore, the prognostic assays described herein can be used to determine whether a subject can be administered a compound (e.g., an agonist, antagonist, peptidomimetic, polypeptide, peptide, nucleic acid, small molecule, or other drug candidate) to treat a disease or disorder associated with an aberrant gelsolin polypeptide or gelsolin-like polypeptide expression or activity. For example, such methods can be used to determine whether a subject can be effectively treated with a compound affecting gelsolin polypeptide levels (e.g., a chemotherapeutic agent). Thus, the invention provides methods for determining whether a subject can be effectively treated with a compound for a disorder or condition associated with an aberrant gelsolin polypeptide or gelsolin-like polypeptide expression or activity in which a test sample is obtained and the gelsolin polypeptide or the gelsolin-like polypeptide is detected using the gelsolin binding agent (e.g., wherein the presence or absence of the gelsolin polypeptide or the gelsolin-like polypeptide is diagnostic for a subject that can be administered the compound to treat a disorder associated with an aberrant gelsolin polypeptide or gelsolin-like polypeptide expression or activity).
The level of the gelsolin polypeptide or the gelsolin-like polypeptide in a urine sample obtained from a subject is determined and compared with the level found in a urine sample obtained from an individual who is free of the disease or condition. An underabundance (or overabundance) of the gelsolin polypeptide or gelsolin-like polypeptide in the sample obtained from the subject suspected of having the disease or condition affecting gelsolin levels compared with the sample obtained from the healthy subject is indicative of the gelsolin polypeptide or gelsolin-like polypeptide-associated disease or condition in the subject being tested. Further testing may be required to make a positive diagnosis. For example, an increased amount of gelsolin or gelsolin-like polypeptide in the urine of a subject compared to a healthy control may indicate the subject is suffering from a disease or condition causing an increased utilization of plasma gelsolin.
There are a number of diseases in which the degree of underabundance (or overabundance) of certain gelsolin polypeptide or gelsolin-like polypeptide molecules known to be indicative of whether a subject with the disease is likely to respond to a particular type of therapy or treatment. Thus, the method of detecting a gelsolin polypeptide or gelsolin-like polypeptide in a sample can be used as a method of prognosis, e.g., to evaluate the likelihood that the subject will respond to the therapy or treatment. Examples of conditions in which plasma gelsolin levels are decreased compared to control subjects include, but are not limited to, septic shock, multiple organ dysfunction syndrome, rheumatoid arthritis, trauma, stroke, heart infarction, cancer, chemotherapy and radiation therapy, systemic autoimmune disease, and chronic hepatitis. Accordingly, urine gelsolin-like polypeptide levels would be increased in subjects suffering from these conditions compared to healthy control subjects.
The methods described herein can be performed, e.g., by utilizing pre-packaged diagnostic kits comprising at least one probe reagent, e.g., gelsolin binding agent described herein, which can be conveniently used, e.g., in clinical settings to diagnose subjects exhibiting symptoms of a disease or illness involving a gelsolin polypeptide or gelsolin-like polypeptide. Furthermore, any cell type or tissue in which gelsolin polypeptide or gelsolin-like polypeptide is expressed can be utilized in the prognostic assays described herein.
Correlating a Subject to a Standard Reference Population.
To deduce a correlation between clinical response to a treatment and a particular level of plasma gelsolin, it is necessary to obtain data on the clinical responses exhibited by a population of individuals who received the treatment, i.e., a clinical population. This clinical data may be obtained by retrospective analysis of the results of a clinical trial(s). Alternatively, the clinical data may be obtained by designing and carrying out one or more new clinical trials. The analysis of clinical population data is useful to define a standard reference population(s) which, in turn, are useful to classify subjects for clinical trial enrollment or for selection of therapeutic treatment. In a preferred embodiment, the subjects included in the clinical population have been graded for the existence of the medical condition of interest. Grading of potential subjects can include, e.g., a standard physical exam or one or more lab tests. Alternatively, grading of subjects can include use of a gene expression pattern. For example, plasma or urine gelsolin levels are useful as grading criteria where there is a strong correlation between expression pattern and susceptibility or severity to a disease or condition. In one embodiment, a subject is classified or assigned to a particular group or class based on similarity between the measured levels of a one or more biomarkers in the subject and the level of the one or more biomarkers observed in a standard reference population.
In one embodiment of the invention, a therapeutic treatment of interest is administered to each subject in a trial population, and each subject's response to the treatment is measured using one or more predetermined criteria. It is contemplated that in many cases, the trial population will exhibit a range of responses, and that the investigator will choose the number of responder groups (e.g., low, medium, high) made up by the various responses. In addition, the expression level of a biomarker (e.g., urine gelsolin) is quantified, which may be done before and/or after administering the treatment. These results are then analyzed to determine if any observed variation in clinical response between groups is statistically significant. Statistical analysis methods, which may be used, are described in L. D. Fisher & G. vanBelle, Biostatistics: A Methodology for the Health Sciences (Wiley-Interscience, New York (1993)).
The skilled artisan can construct a mathematical model that predicts clinical response as a function of expression level from the analyses described above. The identification of an association between a clinical response and an expression level for the biomarker may be the basis for designing a diagnostic method to determine those individuals who will or will not respond to the treatment, or alternatively, will respond at a lower level and thus may require more treatment, i.e., a greater dose of a drug. The diagnostic method may take one of several forms: for example, a ELISA or antibody-based test, a serological test, or a physical exam measurement. The only requirement is that there be a good correlation between the diagnostic test results and the underlying condition. In one embodiment, this diagnostic method uses an antibody assay for serum or urine gelsolin as described above.
In one embodiment, the level of the biomarker molecule in a blood or tissue sample obtained from a first subject is determined and compared with the level found in a blood sample or a sample from the same tissue type obtained from an second subject free of the biomarker-associated disease. An overabundance (or underabundance) of the biomarker molecule in the sample obtained from the first subject suspected of having the biomarker associated disease compared with the sample obtained from the healthy (second) subject is indicative of the biomarker-associated disease in the subject being tested. Further testing may be required to make a positive diagnosis.
In one embodiment, the level of the biomarker molecule (e.g., gelsolin) in a blood, urine, or tissue sample obtained from a subject at a first time point is determined and compared with the level found in a urine sample obtained from the subject at a later time point. An overabundance (or underabundance) of the biomarker molecule in the sample obtained from the subject at the first time point can be compared with the sample obtained from the subject at the second time point wherein the increase of the biomarker level between the first time point compared with the biomarker level at the second time point is indicative of a subject who is in need of gelsolin replacement therapy. Further testing may be required to make a positive diagnosis.
There are a number of diseases in which the degree of overexpression (or underexpression) of certain biomarker molecules is known to be indicative of whether a subject with the disease is likely to respond to a particular type of therapy or treatment. Thus, the method of detecting a biomarker molecule in a sample can be used as a method of prognosis, e.g., to evaluate the likelihood that the subject will respond to the therapy or treatment. Accordingly, in another embodiment, the level of at least one biomarker molecules in a blood or urine sample obtained from a first subject is determined and compared with the level of the at least one biomarker molecules found in a blood or urine sample obtained from a second subject, or standard reference population, responsive to a compound, e.g., a therapeutic compound of interest. Similarity in the level or pattern of expression of the at least one biomarker molecules in a blood or urine sample obtained from a first subject compared with the level of the at least one biomarker molecules found in a blood or urine sample obtained from the second subject, or standard reference population, indicates that the first subject will be responsive to the compound, e.g., therapeutic compound of interest. That is, the level of the relevant biomarker in a suitable tissue or biological sample from the subject is determined and compared with a suitable control, e.g., the level in subjects with the same disease but who have responded favorably to the treatment. The degree to which the biomarker is overexpressed (or underexpressed) in the sample compared with the control may be predictive of likelihood that the subject will not respond favorably to the treatment or therapy (e.g., tolerate chemotherapy). The greater the overexpression (or underexpression) relative to the control, the less likely the subject will respond to the treatment.
There are a number of diseases in which the degree of overexpression (or underexpression) of certain biomarker molecules, i.e., biomarker-associated disease or medical condition, is known to be indicative of whether a subject will develop a disease. Thus, the method of detecting a biomarker in a sample can be used as a method of predicting whether a subject will develop a disease. The level of a one or more biomarkers in a suitable urine or blood sample from a subject at risk of developing the disease or condition is determined and compared with a suitable control, e.g., the level in subjects who are not at risk of developing the disease. The degree to which the one or more biomarkers is overexpressed (or underexpressed) in the sample compared with the control may be predictive of likelihood that the subject will develop the disease. The greater the overexpression (or underexpression) relative to the control, the more likely the subject will development the disease.
The methods described herein can be performed, for example, by utilizing pre-packaged diagnostic kits comprising at least one probe reagent, e.g., anti-gelsolin polypeptide antibody described herein, which can be conveniently used, e.g., in clinical setting to diagnose patients exhibiting symptoms or family history of a disease or illness involving a biomarker of the invention. Furthermore, any cell type or tissue in which a biomarker of the invention is expressed can be utilized in the prognostic assays described herein.
Monitoring Clinical Efficacy.
In one embodiment, the present invention provides for monitoring the influence of agents (e.g., drugs, compounds, or small molecule) on the expression or utilization of gelsolin or gelsolin-like polypeptides. Such assays can be applied in basic drug screening and in clinical trials. For example, the effectiveness of an agent to increase (or decrease) gelsolin or gelsolin-like polypeptide levels can be monitored in clinical trials of subjects exhibiting decreased expression of gelsolin. An agent that affects the expression of gelsolin or gelsolin-like polypeptides can be identified by administering the agent and observing a response. In this way, the expression pattern of the gelsolin or gelsolin-like polypeptide can serve as a marker, indicative of the physiological response of the subject to the agent. Accordingly, this response state may be determined before, and at various points during, treatment of the individual with the agent.
Subject Classification.
Standard control levels of a gelsolin or gelsolin-like polypeptide are determined by measuring levels in different control groups. The control levels (reference levels) are then compared with the measured level of a gelsolin or gelsolin-like polypeptide in a given subject. The subject can be classified or assigned to a particular group based on how similar the measured levels were compared to the reference levels for a given group.
As one of skill in the art will understand, there will be a certain degree of uncertainty involved in making this determination. Therefore, the standard deviations of the control group levels can be used to make a probabilistic determination and the method of this invention are applicable over a wide range of probability-based genotype group determinations. Thus, for example, and not by way of limitation, in one embodiment, if the measured level of the gelsolin polypeptide falls within 2.5 standard deviations of the mean of any of the control groups, then that individual may be assigned to that group. In another embodiment if the measured level of the gelsolin polypeptide falls within 2.0 standard deviations of the mean of any of the control groups then that individual may be assigned to that group. In still another embodiment, if the measured level of the gelsolin polypeptide falls within 1.5 standard deviations of the mean of any of the control groups then that individual may be assigned to that group. In yet another embodiment, if the measured level of the gelsolin polypeptide is 1.0 or less standard deviations of the mean of any of the control groups levels then that individual may be assigned to that group.
Thus, this process allows determination, with various degrees of probability, which group a specific subject should be placed in, and such assignment would then determine the risk category into which the individual should be placed.
B. Kits
Also within the scope of the invention are kits comprising the gelsolin binding agent compositions (e.g., monoclonal antibodies) of the invention and instructions for use. The kits are useful for detecting the presence of a gelsolin polypeptide or a gelsolin-like polypeptide in a biological sample e.g., any body fluid including, but not limited to, e.g., serum, plasma, lymph, cystic fluid, urine, stool, cerebrospinal fluid, acitic fluid or blood and including biopsy samples of body tissue. For example, the kit can comprise: one or more gelsolin binding agents capable of binding a gelsolin polypeptide or a gelsolin-like polypeptide in a biological sample (e.g., an antibody or antigen-binding fragment thereof having the same antigen-binding specificity of antibodies produced by a deposited cell line selected from the group consisting of: CGMCC Accession Nos: 2114, 2115, and 2116); means for determining the amount of the gelsolin polypeptide or gelsolin-like polypeptide in the sample; and means for comparing the amount of the gelsolin polypeptide or the gelsolin-like polypeptide in the sample with a standard. One or more of the gelsolin binding agents may be labeled. The kit components, (e.g., reagents) can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect the gelsolin polypeptide or the gelsolin-like polypeptide.
For antibody-based kits, the kit can comprise, e.g., 1) a first antibody, e.g., attached to a solid support, which binds to a polypeptide corresponding to a marker or the invention; and, optionally; 2) a second, different antibody which binds to either the polypeptide or the first antibody and is conjugated to a detectable label.
The kit can also comprise, e.g., a buffering agent, a preservative or a protein-stabilizing agent. The kit can further comprise components necessary for detecting the detectable-label, e.g., an enzyme or a substrate. The kit can also contain a control sample or a series of control samples, which can be assayed and compared to the test sample. Each component of the kit can be enclosed within an individual container and all of the various containers can be within a single package, along with instructions for interpreting the results of the assays performed using the kit. The kits of the invention may contain a written product on or in the kit container. The written product describes how to use the reagents contained in the kit, e.g., to use the biomarkers of the present invention in determining a strategy for preventing or treating a medical condition in a subject. In several embodiments, the use of the reagents can be according to the methods of the invention.
C. Prophylactic and Therapeutic Use of Gelsolin Replacement Therapy
General. The gelsolin binding agents and methods of the present invention can be used in conjunction with gelsolin replacement therapy. Specifically, the invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with an aberrant gelsolin expression or activity. The gelsolin binding agents and methods are used, for example, to ascertain the suitability of gelsolin replacement therapy for a subject or monitor the efficacy of gelsolin replacement therapy in a subject receiving such therapy. In one embodiment, a therapeutically effective amount of recombinant or purified, native gelsolin compounds are administered so as to provide therapeutic benefits against the secondary toxic effects of excessive extracellular actin. By “excessive” extracellular actin is meant an amount of extracellular actin which exceeds the ability of the plasma proteins to bind and clear the actin from extracellular fluids without secondary tissue damage or toxic effects. By “secondary” tissue damage or toxic effects is meant the tissue damage or toxic effects which occur to otherwise healthy tissues, organs, and the cells therein, due to the presence of excessive extracellular actin in the plasma, usually as a result of a “primary” tissue injury elsewhere in the body. While not wishing to be limited by theory, infusion of gelsolin, results in a) binding to actin monomers so as to prevent their condensation into actin filaments, and/or b) cleavage of actin filaments to the monomeric state, and/or c) enhanced clearance of such actin complexed to actin-binding proteins or fragments thereof from the circulation or extracellular tissue environment.
Optionally, the administration is made during the course of adjunct therapy such as combined cycles of radiation, chemotherapeutic treatment, or administration of other cytoprotective or immunomodulatory agent. As such the binding agents of the present invention and a compound useful in adjunct therapy may be administrated simultaneously and sequentially to a subject in need of administration thereof.
In one aspect, the invention provides a method for preventing, in a subject, a disease or condition associated with an aberrant gelsolin expression or activity, by administering to the subject gelsolin. Administration of a prophylactic gelsolin binding agent can occur prior to the manifestation of symptoms characteristic of the aberrancy, such that a disease or disorder is prevented or, alternatively, delayed in its progression. In therapeutic applications, gelsolin is administered to a subject suspected of, or already suffering from, reduced serum gelsolin levels. An amount adequate to accomplish therapeutic or prophylactic treatment is defined as a therapeutically- or prophylactically-effective dose.
Determination of the Biological Effect of the gelsolin-Binding Agent-Based Therapeutic.
In various embodiments of the invention, suitable in vitro or in vivo assays are performed to determine the effect of gelsolin replacement therapy and whether its administration is indicated for treatment of the affected tissue in a subject.
Typically, an effective amount of gelsolin, sufficient for achieving a therapeutic or prophylactic effect, range from about 0.000001 mg per kilogram body weight per day to about 10,000 mg per kilogram body weight per day. Preferably, the dosage ranges are from about 0.0001 mg per kilogram body weight per day to about 100 mg per kilogram body weight per day. For administration of gelsolin, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 5 mg/kg every week, every two weeks or every three weeks, of the host body weight. For example dosages can be 1 mg/kg body weight or 10 mg/kg body weight every week, every two weeks or every three weeks or within the range of 1-10 mg/kg every week, every two weeks or every three weeks. An exemplary treatment regime entails administration once per every two days or once a week or once every month. Gelsolin is usually administered on multiple occasions. Intervals between single dosages can be daily, weekly, monthly or yearly. Intervals can also be irregular as indicated by measuring blood levels of antibody in the subject. In some methods, dosage is adjusted to achieve a serum gelsolin concentration in the subject of from about 75 μg/mL to about 125 μg/mL, 100 μg/mL to about 150 μg/mL, from about 125 μg/mL to about 175 μg/mL, or from about 150 μg/mL to about 200 μg/mL. Alternatively, gelsolin can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the gelsolin binding agent in the subject. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some subjects continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the subject shows partial or complete amelioration of symptoms of disease. Thereafter, the patent can be administered a prophylactic regime.
Toxicity.
Preferably, an effective amount (e.g., dose) of gelsolin described herein will provide therapeutic benefit without causing substantial toxicity to the subject. Toxicity of the gelsolin described herein can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., by determining the LD50 (the dose lethal to 50% of the population) or the LD100 (the dose lethal to 100% of the population). The dose ratio between toxic and therapeutic effect is the therapeutic index. The data obtained from these cell culture assays and animal studies can be used in formulating a dosage range that is not toxic for use in human. The dosage of the gelsolin described herein lies preferably within a range of circulating concentrations that include the effective dose with little or no toxicity. The dosage can vary within this range depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the subject's condition. See, e.g., Fingl et al., In: The Pharmacological Basis of Therapeutics Ch. 1 (1975).
Formulations of Pharmaceutical Compositions.
According to the methods of the present invention, the gelsolin can be incorporated into pharmaceutical compositions suitable for administration. The pharmaceutical compositions generally comprise recombinant or substantially purified native gelsolin and a pharmaceutically-acceptable carrier in a form suitable for administration to a subject. Pharmaceutically-acceptable carriers are determined in part by the particular composition being administered, as well as by the particular method used to administer the composition. Accordingly, there is a wide variety of suitable formulations of pharmaceutical compositions for administering the protein compositions. (See, e.g., Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa. 18th ed. (1990).) The pharmaceutical compositions are generally formulated as sterile, substantially isotonic and in full compliance with all Good Manufacturing Practice (GMP) regulations of the U.S. Food and Drug Administration.
The terms “pharmaceutically-acceptable,” “physiologically-tolerable,” and grammatical variations thereof, as they refer to compositions, carriers, diluents and reagents, are used interchangeably and represent that the materials are capable of administration to or upon a subject without the production of undesirable physiological effects to a degree that would prohibit administration of the composition. For example, “pharmaceutically-acceptable excipient” means an excipient that is useful in preparing a pharmaceutical composition that is generally safe, non-toxic, and desirable, and includes excipients that are acceptable for veterinary use as well as for human pharmaceutical use. Such excipients can be solid, liquid, semisolid, or, in the case of an aerosol composition, gaseous. “Pharmaceutically-acceptable salts and esters” means salts and esters that are pharmaceutically-acceptable and have the desired pharmacological properties. Such salts include salts that can be formed where acidic protons present in the gelsolin are capable of reacting with inorganic or organic bases. Suitable inorganic salts include those formed with the alkali metals, e.g., sodium and potassium, magnesium, calcium, and aluminum. Suitable organic salts include those formed with organic bases such as the amine bases, e.g., ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine, and the like. Such salts also include acid addition salts formed with inorganic acids (e.g., hydrochloric and hydrobromic acids) and organic acids (e.g., acetic acid, citric acid, maleic acid, and the alkane- and arene-sulfonic acids such as methanesulfonic acid and benzenesulfonic acid). Pharmaceutically-acceptable esters include esters formed from carboxy, sulfonyloxy, and phosphonoxy groups present in the gelsolin, e.g., C1-6 alkyl esters. When there are two acidic groups present, a pharmaceutically-acceptable salt or ester can be a mono-acid-mono-salt or ester or a di-salt or ester; and similarly where there are more than two acidic groups present, some or all of such groups can be salified or esterified. The gelsolin polypeptide can be present in unsalified or unesterified form, or in salified and/or esterified form, and the naming of such gelsolin polypeptide is intended to include both the original (unsalified and unesterified) compound and its pharmaceutically-acceptable salts and esters. Also, certain gelsolin polypeptides can be present in more than one stereoisomeric form, and the naming of such gelsolin polypeptide is intended to include all single stereoisomers and all mixtures (whether racemic or otherwise) of such stereoisomers. A person of ordinary skill in the art, would have no difficulty determining the appropriate timing, sequence and dosages of administration for particular drugs and compositions of the present invention.
Examples of such carriers or diluents include, but are not limited to, water, saline, Ringer's solutions, dextrose solution, and 5% human serum albumin. Liposomes and non-aqueous vehicles such as fixed oils may also be used. The use of such media and compounds for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or compound is incompatible with the gelsolin binding agent, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions.
A pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration. The gelsolin compositions can be administered by parenteral, topical, intravenous, oral, subcutaneous, intraarterial, intradermal, transdermal, rectal, intracranial, intraperitoneal, intranasal; intramuscular route or as inhalants. The gelsolin can optionally be administered in combination with other agents that are at least partly effective in treating various diseases including various actin- or microfilament-related diseases.
Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial compounds such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfate; chelating compounds such as ethylenediaminetetraacetic acid (EDTA); buffers such as acetates, citrates or phosphates, and compounds for the adjustment of tonicity such as sodium chloride or dextrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringeability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, e.g., water, ethanol, polyol (e.g., glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, e.g., by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal compounds, e.g., parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic compounds, e.g., sugars, polyalcohols such as manitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition a compound which delays absorption, e.g., aluminum monostearate and gelatin.
Sterile injectable solutions can be prepared by incorporating the gelsolin in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the agent into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, methods of preparation are vacuum drying and freeze-drying that yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. The agents can be administered in the form of a depot injection or implant preparation which can be formulated in such a manner as to permit a sustained or pulsatile release of the active ingredient.
Oral compositions generally include an inert diluent or an edible carrier. They can be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the polypeptide can be incorporated with excipients and used in the form of tablets, troches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed. Pharmaceutically compatible binding compounds, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating compound such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening compound such as sucrose or saccharin; or a flavoring compound such as peppermint, methyl salicylate, or orange flavoring.
For administration by inhalation, the gelsolin is delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, e.g., for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the gelsolin is formulated into ointments, salves, gels, or creams as generally known in the art.
The gelsolin can also be prepared as pharmaceutical compositions in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
In one embodiment, the gelsolin is prepared with carriers that will protect the gelsolin against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically-acceptable carriers. These can be prepared according to methods known to those skilled in the art, e.g., as described in U.S. Pat. No. 4,522,811.
Preparation of Recombinant Gelsolin.
Most of the discussion below pertains to production of gelsolin by culturing cells transformed with a vector containing a nucleic acid encoding gelsolin and recovering the polypeptide from the cell culture. It is further envisioned that the gelsolin may be produced by purifying native gelsolin from plasma using affinity purification (described above). The proteins and DNA sequences encoding the proteins may be produced using standard methods. Purification can also be accomplished using standard procedures such as isolating proteins using chromatography, using antibodies directed against the polypeptides, or by producing the polypeptides in a form in which they are fused to a moiety (or tag) that aids in purification and which can then be cleaved.
A polynucleotide encoding a gelsolin or gelsolin-like polypeptide (for example, the polypeptide of SEQ ID NO.: 1) may be inserted into any of the many commercially available expression vectors using reagents and techniques that are well known in the art. In preparing the recombinant expression constructs, the various polynucleotides of the present invention may be inserted or substituted into a bacterial plasmid-vector. Any convenient plasmid may be employed, which will be characterized by having a bacterial replication system, a marker which allows for selection in a bacterium and generally one or more unique, conveniently located cloning sites. Numerous plasmids, also referred to as vectors, are available for transformation. Suitable vectors include, but are not limited to, the following: viral vectors, such as lambda vector system gt11, Charon 4, and plasmid vectors such as pBR322, pBR325, pACYC177, pACYC1084, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pKC37, pKC101, SV 40, pBluescript II SK+/−. or KS+/−(Stratagene, La Jolla, Calif.), and any derivatives thereof. Also suitable are yeast expression vectors, which may be highly useful for cloning and expression. Exemplary yeast plasmids include, without limitation, pPICZ, and pFLD. (Invitrogen, Carlsbad, Calif.). The selection of a vector will depend on the preferred transformation technique and target host cells.
The nucleic acid molecule encoding gelsolin is inserted into a vector in the 5′ to 3′ direction, such that the open reading frame is properly oriented for the expression of the encoded protein under the control of a promoter of choice. In this way, the gelsolin structural gene is said to be “operably linked” to the promoter. Single or multiple nucleic acids may be inserted into an appropriate vector in this way, each under the control of suitable promoters, to prepare a nucleic acid construct of the present invention.
Certain regulatory sequences may also be incorporated into the expression constructs of the present invention. These include non-transcribed regions of the vector, which interact with host cellular proteins to carry out transcription and translation. Such elements may vary in their strength and specificity. Depending on the vector system and host utilized, any number of suitable transcription and/or translation elements, including constitutive, inducible, and repressible promoters, as well as minimal 5′ promoter elements may be used.
A constitutive promoter is a promoter that directs constant expression of a gene in a cell. Examples of some constitutive promoters that are widely used for inducing expression of heterologous polynucleotides include the ADH1 promoter for expression in yeast, those derived from any of the several actin genes, which are known to be expressed in most eukaryotic cell types, and the ubiquitin promoter, which is the promoter of a gene product known to accumulate in many cell types. Examples of constitutive promoters for use in mammalian cells include the RSV promoter derived from Rous sarcoma virus, the CMV promoter derived from cytomegalovirus, β-actin and other actin promoters, and the EF1α promoter.
Also suitable as a promoter in the plasmids of the present invention is a promoter that allows for external control over the regulation of gene expression. One way to regulate the amount and the timing of gene expression is to use an inducible promoter. Unlike a constitutive promoter, an inducible promoter is not always optimally active. An inducible promoter is capable of directly or indirectly activating transcription of one or more DNA sequences or genes in response to an inducing agent (or inducer). Some inducible promoters are activated by physical means, such as the heat shock promoter (HSP), which is activated at certain temperatures. Other promoters are activated by a chemical means, for example, IPTG. Other examples of inducible promoters include the metallothionine promoter, which is activated by heavy metal ions, and hormone-responsive promoters, which are activated by treatment of certain hormones. In the absence of an inducer, the nucleic acid sequences or genes under the control of the inducible promoter will not be transcribed or will only be minimally transcribed. Promoters of the nucleic acid construct of the present invention may be either homologous (derived from the same species as the host cell) or heterologous (derived from a different species than the host cell).
Once the nucleic acid construct of the present invention has been prepared, it may be incorporated into a host cell. This is carried out by transforming or transfecting a host or cell with a plasmid construct of the present invention, using standard procedures known in the art, such as described by Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, New York (2001). Suitable hosts and cells for the present invention include, without limitation, bacterial cells, virus, yeast cells, insect cells, plant cells, and mammalian cells, including human cells, as well as any other cell system that is suitable for producing a recombinant protein. Exemplary bacterial cells include, without limitation, E. coli and Mycobacterium sp. Exemplary yeast hosts include without limitation, Pischia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Methods of transformation or transfection may result in transient or stable expression of the genes of interest contained in the plasmids. After transformation, the transformed host cells can be selected and expanded in suitable culture. Transformed cells are first identified using a selection marker simultaneously introduced into the host cells along with the nucleic acid construct of the present invention. Suitable markers include markers encoding for antibiotic resistance, such as resistance to kanamycin, gentamycin, ampicillin, hygromycin, streptomycin, spectinomycin, tetracycline, chloramphenicol, and the like. Any known antibiotic-resistance marker can be used to transform and select transformed host cells in accordance with the present invention. Cells or tissues are grown on a selection medium containing an antibiotic, whereby generally only those transformants expressing the antibiotic resistance marker continue to grow. Additionally, or in the alternative, reporter genes, including, but not limited to, β-galactosidase, β-glucuronidase, luciferase, green fluorescent protein (GFP) or enhanced green fluorescent protein (EGFP), may be used for selection of transformed cells. The selection marker employed will depend on the target species.
To obtain the gelsolin protein, expression is induced if the coding sequences is under the control of an inducible promoter. To isolate the protein, the host cell carrying an expression vector is propagated, homogenized, and the homogenate is centrifuged to remove bacterial debris. The supernatant is then subjected to sequential ammonium sulfate precipitation. The fraction containing the protein of the present invention is subjected to gel filtration in an appropriately sized dextran or polyacrylamide column to separate the proteins. If necessary, the protein fraction may be further purified by HPLC. Alternative methods of protein purification may be used as suitable. See J. E. Coligan et al., eds., Current Protocols in Protein Science (John Wiley & Sons (2003)). Upon obtaining the substantially purified recombinant protein, the protein may be administered to a subject as described herein. [Alternatively, recombinant gelsolin may be purified using standard anion exchange chromatography. Oberley, R. E. et al., Am J Physiol Lung Cell Mol Physiol 287:L296-306 (2004).]
The following EXAMPLES are presented in order to more fully illustrate some embodiments of the invention. These EXAMPLES should in no way be construed as limiting the scope of the invention, as defined by the appended claims.
The present invention is further illustrated by the following examples, which should not be construed as limiting in any way.
The monoclonal anti-gelsolin antibodies GN3E9, GC1C10, and GF2D6 were prepared as described in PCT Application No. PCT/CN2007/002467. The isotype of selected anti-gelsolin antibodies was determined by goat anti-murine isotype specific antibodies (SouthernBiotech, Birmingham, Ala.). The isotype of GN3E9 was determined as murine IgG2b kappa, and the isotype of GC and GF2D6 were determined as murine IgG1 kappa.
GN3E9 was purified by affinity chromatography using Sepharose GL-4B affinity purification medium (Pharmacia, Uppsala, Sweden). The GC1C10 and GF2D6 antibodies were purified by affinity chromatograpy using Protein G-Sepharose CL-4B affinity purification medium (Pharmacia, Uppsala, Sweden). The culture supernatants were applied to the column with a flow rate of 2 ml per min. After the culture supernatant was passed through the column, it was washed with 50 ml PBS. The protein was eluted with elution buffer (0.1 M glycine (pH 2.4), 0.15 M NaCl). The optical density of each eluted fraction (1 ml) was measured at OD280 nm. The fractions with OD280>0.1 units were collected. After addition of 100 μl of neutralization buffer (1M Tris-HCl pH 8.5) to the fraction, the eluates were placed separately in dialysis tubing, and the eluates dialyzed against 1 L of PBS (pH 7.5) at 4° C. The dialysis buffer was changed twice. Each affinity purified antibody was concentrated to 1 mg/ml, sterilized and stored at 4° C. until use.
Immunoprecipitation of Urine Gelsolin Polypeptides.
To determine the ability of gelsolin binding agents of the invention to immunoprecipitate the urine form(s) of gelsolin, GN3E9 and GC antibodies were conjugated to CNBr-activated Sepharose 4B (Amersham Pharmacia Biotech (Piscataway, N.J.), at a concentration of 2 mg/ml beads. The conjugation procedure was performed according to the manufacturer's instructions. Briefly, the pre-activated beads (660 mg; equal to approximately 2 mL final bead volume) were suspended in 15 volumes of 1 mM HCl and allowed to swell for 30 min. The beads were then washed with 15 gel volumes of cold (4° C.) 1 mM HCl followed by a wash with 15 volumes of coupling buffer (0.1M NaHCO3 pH 8.3 containing 0.5 M NaCl) to yield beads that are referred to as “washed gel”. Each anti-gelsolin antibody (GN3E9, GC1C10, and GF2D6) was diluted in coupling buffer to 0.5 to 1.0 mg/ml and the pH was adjusted to pH 8.3. The washed gel was added to each anti-gelsolin antibody solution and the mixtures were incubated overnight at 4° C. to yield “coupled gels”. The coupled gels were resuspended in 15 volumes of 1 M ethanolamine for 2-4 h at room temperature to block unused activated chemical conjugation sites on the activated beads. The blocked gels were then washed 8 times in 15 volumes with alternating 50 mM Tris, 1 M NaCl pH 8.0 and 50 mM glycine, 1 M NaCl pH 3.5 buffers followed by a final wash with 10 gel volumes of PBS to remove any unbound material.
Next, one milliliter (1 mL) human urine samples, which had been centrifuged briefly to remove sediment, were incubated with 10 μl anti-gelsolin antibody-conjugated beads (i.e., GN3E9 or GC conjugated beads) at room temperature for 2 h. Unbound material was rinsed from the anti-gelsolin antibody-conjugated beads or blank beads by pelleting the bead by centrifugation (14,000 rpm, 3 min), removing the supernatant and then washing the pelleted beads by resuspension in PBS. Following five (5) wash cycles, material bound to the anti-gelsolin antibody-conjugated beads was removed under denaturing conditions by adding 40 μl SDS-PAGE loading buffer (SDS-PAGE loading buffer prepared by mixing 3× stock: 1M Tris-Cl pH 6.8 2.4 ml; 20% SDS 3 ml; Glycerol (100%) 3 ml; B-mercaptoethanol 1.6 ml; Bromophenol blue 0.006 g, 10 ml) to the pelleted beads and boiling the sample for 5 min. The immunoprecipitated proteins were fractionated on a 10% SDS-PAGE and visualized stained with Coomassie blue stain using standard techniques. After destain, the gel was scanned using an HP photographic scanner. The results are shown in
The C-terminal specific anti-gelsolin antibodies of the invention (GC1C10 and GF2D6) were able to immunoprecipitate two ˜50 kDa polypeptides from ICU patients, but not from healthy control patients. The N-terminal specific anti-gelsolin antibody GN3E9 was unable to immunoprecipitate these fragments from either control or ICU patients. As such, the gelsolin binding agents of the invention tested in the present studies were able to identify ˜50 kDa immunoreactive polypeptides from human urine samples. The identity of these ˜50 kDa polypeptides was confirmed to be C-terminal gelsolin fragments by mass spectrometry analysis. (See Example 2.)
The ability of the select gelsolin binding agents of the invention tested to immunoprecipitate gelsolin-like polypeptide is advantageous for use of these binding agents in methods of the present invention. Specifically, gelsolin binding agents of the invention which can precipitate immunoreactive gelsolin polypeptides can be employed to detect gelsolin-like fragments in urine samples.
Western Blot Analysis.
A western blot analysis technique was used to assess the immunopreciptiation of urine gelsolin fragments from the biological samples. That is, to further determine the identity of the urine gelsolin fragments, western blot analysis of immunoprecipitated human urine proteins with anti-gelsolin antibodies was performed. The urine samples of three normal human subjects and three severe stroke (ICU) subjects were immunoprecipitated with the C-terminal-specific anti-gelsolin antibodies GN3E9 or GC1C10 as described above. Immunoprecipitated proteins were fractionated by 10% SDS-PAGE and western blotted onto nitrocellulose membrane using standard techniques. After blocking the electroblotted nitrocellulose membrane (blot) using 5% (w/v) non-fat milk at room temperature for 1 h, the blots were probed with the purified anti-gelsolin antibodies GN3E9 or GC1C10 coupled to HRP (1 μg/ml) at room temperature for 2 h. Unbound anti-gelsolin antibody was rinsed from the blots by washing with PBS containing 0.02% Tween 20 at room temperature for 10 min with shaking. The complexes were visualized using HRP-mediated chemiluminescence. Specifically, the blots were incubated with LumiGLO® Peroxidase Chemiluminescent Substrate (KPL, Gaithersburg, Md.) for 3 min, and exposed to X-ray films. The results are shown in
To confirm that the proteins immunoprecipitated by anti-gelsolin antibodies are fragments of human gelsolin, protein bands were cut from SDS-PAGE and the contents subjected to analysis by mass spectroscopy at the National Center of Biomedical Analysis (Beijing, China) using standard techniques (see Lewis et al., Identification of Viral Mutants by Mass Spectrometry, Proc Nat Acad Sci USA 95:8596-8601 (1998)). Urine proteins were immunoprecipitated as described in Example 1 using C-terminal specific anti-gelsolin antibody GF2D6. The individual protein bands that were analyzed are shown in
RIEGSNKVPV DPATYGQFYG GDSYIILYNY RHGGRQGQII YNWQGAQSTQ
DEVAASAILT AQLDEELGGT PVQSRVVQGK EPAHLMSLFG GKPMIIYKGG
AAYLWVGTGA SEAEKTGAQE LLRVLRAQPV QVAEGSEPDG FWEALGGKAA
RIEGSNKVPV DPATYGQFYG GDSYIILYNY RHGGRQGQII YNWQGAQSTQ
DEVAASAILT AQLDEELGGT PVQSRVVQGK EPAHLMSLFG GKPMIIYKGG
AAYLWVGTGA SEAEKTGAQE LLRVLRAQPV QVAEGSEPDG FWEALGGKAA
A proteomics database (Swiss-Prot/TrEMBL) search indicated that the polypeptides were C-terminal fragments of human gelsolin. N-terminal sequence of the F1 fragment was obtained and showed that the F1 fragment corresponds to amino acids 410-782 of SEQ ID NO: 1. Accordingly, the ˜50 kDa polypeptides which were immunoprecipitated by the anti-gelsolin antibodies of the invention were confirmed as C-terminal fragments human gelsolin.
Immunoprecipitation of Urine Gelsolin C-Terminal Fragments. To determine the ability of gelsolin binding agents of the invention to immunoprecipitate fragment(s) of urine gelsolin, the GC5D1 antibody was conjugated to CNBr-activated Sepharose 4B (Amersham Pharmacia Biotech (Piscataway, N.J.), at a concentration of 2 mg/ml beads. The conjugation procedure was performed according to the manufacturer's instructions. Briefly, the pre-activated beads (660 mg; equal to approximately 2 mL final bead volume) were suspended in 15 volumes of 1 mM HCl and allowed to swell for 30 min. The beads were then washed with 15 gel volumes of cold (4° C.) 1 mM HCl followed by a wash with 15 volumes of coupling buffer (0.1M NaHCO3 pH 8.3 containing 0.5 M NaCl) to yield beads that are referred to as “washed gel”. The GC5D1 antibody was diluted in coupling buffer to 0.5 to 1.0 mg/ml and the pH was adjusted to pH 8.3. The washed gel was added to each anti-gelsolin antibody solution and the mixtures were incubated overnight at 4° C. to yield “coupled gels”. The coupled gels were resuspended in 15 volumes of 1 M ethanolamine for 2-4 h at room temperature to block unused activated chemical conjugation sites on the activated beads. The blocked gels were then washed 8 times in 15 volumes with alternating 50 mM Tris, 1 M NaCl pH 8.0 and 50 mM glycine, 1 M NaCl pH 3.5 buffers followed by a final wash with 10 gel volumes of PBS to remove any unbound material.
Next, one milliliter (1 mL) human urine samples from controls and severe stroke patients, which had been centrifuged briefly to remove sediment, was incubated with 10 μl GC5D1 antibody-conjugated beads at room temperature for 2 h. Unbound material was rinsed from the anti-gelsolin antibody-conjugated beads by pelleting the bead by centrifugation (14,000 rpm, 3 min), removing the supernatant and then washing the pelleted beads by resuspension in PBS. Following five (5) wash cycles, materials bound to the GC5D 1 antibody-conjugated beads was removed under denaturing conditions by adding 40 μl SDS-PAGE loading buffer (SDS-PAGE loading buffer prepared by mixing 3× stock: 1M Tris-Cl pH 6.8 2.4 ml; 20% SDS 3 ml; Glycerol (100%) 3 ml; B-mercaptoethanol 1.6 ml; Bromophenol blue 0.006 g, 10 ml) to the pelleted beads and boiling the sample for 5 min. The immunoprecipitated proteins were fractionated on a 10% SDS-PAGE and western blotted onto nitrocellulose membrane using standard techniques. After blocking the electroblotted nitrocellulose membrane (blot) using 5% (w/v) non-fat milk at room temperature for 1 h, the blots were probed with GF2D6 coupled to HRP (1 μg/ml) at room temperature for 2 h. Unbound anti-gelsolin antibody was rinsed from the blots by washing with PBS containing 0.02% Tween 20 at room temperature for 10 min with shaking. The complexes were visualized using HRP-mediated chemiluminescence. Specifically, the blots were incubated with LumiGLO® Peroxidase Chemiluminescent Substrate (KPL, Gaithersburg, Md.) for 3 min, and exposed to X-ray films. The results are shown in
The C-terminal specific anti-gelsolin antibodies of the invention (GC5D1, GF2D6) were able to immunoprecipitated and detect two ˜50 kDa polypeptides from ICU patients, but not from healthy control patients. The full-length gelsolin polypeptide was not detected using the combination of these two antibodies. As such, the gelsolin binding agents of the invention tested in the present studies were able to identify ˜50 kDa immunoreactive polypeptides from human urine samples.
The ability of the select gelsolin binding agents of the invention tested to immunoprecipitate is advantageous for use of these binding agents in methods of the present invention. Specifically, gelsolin binding agents of the invention which can precipitate immunoreactive gelsolin polypeptides can be employed to detect gelsolin-like polypeptides in urine samples.
Varying concentrations (0.01 to 1,000 ng/mL) of recombinant gelsolin immunogens were added to the ELISA plates. The immunogens were either full-length gelsolin (FL), an N-terminal (NT) fragment comprising amino acids 13 to 440 of SEQ ID NO: 1, or a C-terminal fragment (CT) comprising amino acids 440 to 782 of SEQ ID NO: 1. Unbound gelsolin immunogen was rinsed from the wells by washing three times with PBS.
Bound gelsolin immunogen was detected by incubating wells (30 min; 37° C.) with HRP-conjugated 2D6 antibody (1:10,000). Unbound HRP-conjugated 2D6 antibody was removed by rinsing the wells three times with PBS (5 min each). The antibody complexes were measured using SureBlue TMB 1-Component Microwell Peroxidase Substrate (KPL, Gaithersburg, Md.). Specifically, SureBlue TMB 1-Component Microwell Peroxidase Substrate (KPL, Gaithersburg, Md.) was added to the wells and the plate was incubated for 10 min to allow HRP-mediated conversion of the substrate. The enzymatic reaction was stopped with the addition of 100 μl 2N H2SO4. The optical density of the sample wells was then measured at 450 nm/650 nm using an ELISA plate reader.
A summary of the results of studies performed to determine the binding characteristics of select gelsolin binding agents as detailed below are shown in
To test the ability of the gelsolin ELISA assay to quantitate urine gelsolin fragments in a clinical setting, samples from normal patients and trauma patients were obtained and analyzed using an ELISA assay as described in Example 5. Samples were obtained from subjects suffering from various kinds of trauma or medical conditions. These included critical care (ICU) patients and cancer patients (i.e. patients with newly diagnosed cancer prior to any major treatment such as surgery, chemotherapy, or radiation therapy). Further samples were obtained from patients having major surgery, where major surgery is defined as any surgical procedure that involves anesthesia or respiratory assistance. The criteria for patient recruitment was previously described. (See Wang et al., Eur J Clin Pharmacol 62:927-31 (2006).) Sepsis patents included those patients with sepsis associated with new organ dysfunction, hypotension, or hypoperfusion. The criteria for patient recruitment was previously described. (See Chen et al., Genes Immun 8:439-43 (2007).) Finally, samples were obtained from patients having nephritis, which is an inflammation of the kidney.
The results are shown in
In this example, the relationship between full-length plasma gelsolin levels and urine gelsolin fragments was investigated using patient-matched samples from either normal individuals or critical care patients. The level of urine gelsolin fragments was measured as described in
Gelsolin ELISA for plasma samples was carried out as follows. An ELISA plate (96 well; BD biosciences CA) was coated with 10 μg/ml of the capture antibody GN3E9 at 4° C. overnight. Unbound capture antibody was rinsed from the wells by washing the plate three times with PBS. Non-specific binding sites were then blocked by incubating the wells with 3% (w/v) BSA in PBS (1 h, room temperature). Blocking solution was removed from the wells and the plate was air dried prior to vacuum sealing and storage at 4° C. prior to use. Fifty (50) μl of human plasma was first added to appropriate wells of the gelsolin ELISA plate treated with capture antibody which had been equilibrated to room temperature. Immediately following the addition of the samples to the plate, 50 μl of HRP-conjugated detection antibody GC1C10 (˜0.1 μg/ml in blocking buffer) was added to appropriate wells and the plate was incubated for 20 min at 37° C. Unbound material was rinsed from the wells by washing the plate three times with PBS. Captured plasma gelsolin: HRP-conjugated antibody complexes were measured by adding 100 μl ECL substrate buffer (KPL, Inc., Gaithersburg, Md.) was added. After incubation at 37° C. for 3 min, the optical density of each well was measured at 450 nm/650 nm in an ELISA plate reader.
The results are shown in
To test the ability of the gelsolin ELISA assay to quantitate urine gelsolin fragments in a clinical setting, samples from critical care patients are obtained and analyzed as described above. Clinical outcome or disease stage for each patient is assessed. A mathematical model is constructed that predicts clinical response or outcome as a function of urine gelsolin fragment level. The identification of an association between clinical response and a level for the urine gelsolin fragments is a basis for designing a diagnostic method to determine those subjects who will or will not respond to the treatment, or alternatively, will respond at a lower level and thus require more treatment, i.e., a greater dose of a compound, drug, or treatment. Using the correlations measured by the mathematical model, gelsolin ELISA using gelsolin binding agents of the invention is useful to determine clinical outcome of patients.
The present invention is not to be limited in terms of the particular embodiments described in this application, which are intended as single illustrations of individual aspects of the invention. Many modifications and variations of this invention can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. Functionally equivalent methods and compositions within the scope of the invention, in addition to those enumerated herein, will be apparent to those skilled in the art from the foregoing descriptions. Such modifications and variations are intended to fall within the scope of the appended claims. The present invention is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled. It is to be understood that this invention is not limited to particular methods, reagents, compounds compositions or biological systems, which can, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.
Other embodiments are set forth within the following claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/CN2007/002467 | Aug 2007 | CN | national |
This application claims priority to PCT Application No. PCT/CN2007/002467, filed Aug. 15, 2007, the contents of which are incorporated herein in its entirety.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/CN2008/072005 | 8/15/2008 | WO | 00 | 2/11/2010 |
