Claims
- 1. An isolated nucleic acid sequence encoding for a recombinant, replication-deficient adenovirus and a glycosyltransferase, wherein said glycosyltransferase is selected from the group consisting of α2,3-ST glycosyltransferase, α2,6-ST glycosyltransferase, HexB glycosyltransferase, Fuco glycosyltransferase, GnTIII glycosyltransferase, GnTI glycosyltransferase, SLex-ST glycosyltransferase and GnTV glycosyltransferase.
- 2. The isolated nucleic acid of claim 1, wherein the expression of said glycosyltransferase gene is under transcriptional control of a regulator selected from the group consisting of CMV immediate-early enhancer/promoter, SV40 early enhancer/promoter, JC polyomavirus promoter, and chicken β-actin promoter.
- 3. The isolated nucleic acid of claim 2, wherein the glycosyltransferase is α2,6-ST glycosyltransferase.
- 4. A method for altering the expression of a glycosyltransferase within a cell, comprising stablely transfecting the isolated nucleic acid of claim 3 into the cell, such that the expression of the glycosyltransferase within the cell is altered.
- 5. The method of claim 4, wherein the expression of the glycosyltransferase is increased.
- 6. The method of claim 5, wherein the cell is a brain cancer cell.
- 7. The method of claim 6, wherein the brain cancer cell is a glioma cell.
- 8. The method of claim 7, wherein the brain cancer cell is a meningioma cell.
- 9. The method of claim 6, wherein the isolated nucleic acid is transfected ex vivo.
- 10. The method of claim 6, wherein the isolated nucleic acid is transfected in vivo.
- 11. A recombinant adenoviral particle containing a nucleic acid encoding for a glycosyltransferase wherein said nucleic acid sequence is selected from the group consisting of nucleic acid sequence encoding for α2,3-ST glycosyltransferase, α2,6-ST glycosyltransferase, HexB glycosyltransferase, Fuco glycosyltransferase, GnTIII glycosyltransferase, GnTI glycosyltransferase, SLex-ST glycosyltransferase and GnTV glycosyltransferase.
- 12. The adenoviral particle of claim 12, wherein the expression of said nucleic acid encoding for a glycosyltransferase is under transcriptional control of a regulator selected from the group consisting of CMV immediate-early enhancer/promoter, SV40 early enhancer/promoter, JC polyomavirus promoter, and chicken β-actin promoter.
- 13. The adenoviral particle of claim 13, wherein the glycosyltransferase is α2,6-ST glycosyltransferase.
- 14. A method for altering the expression of a glycosyltransferase within a cell, comprising introducing the adenoviral particle of claim 3 into the cell, such that the expression of the glycosyltransferase within the cell is altered.
- 15. The method of claim 14, wherein the expression of the glycosyltransferase is increased.
- 16. The method of claim 15, wherein the cell is a brain cancer cell.
- 17. The method of claim 16, wherein the brain cancer cell is a glioma cell.
- 18. The method of claim 17, wherein the brain cancer cell is a meningioma cell.
- 19. The method of claim 16, wherein the isolated nucleic acid is transfected ex vivo.
- 20. The method of claim 16, wherein the isolated nucleic acid is transfected in vivo.
Parent Case Info
[0001] This application is a divisional application of application Ser. No. 09/597,604, filed Jun. 20, 2000, which is a continuation-in-part of application Ser. No. 08/969,437 filed Nov. 11, 1997, application Ser. No. 09/597,604 claims the benefit of Provisional Application No. 60/171,728, filed Dec. 22, 1999. The instant application claims the benefit of all the listed applications, which are hereby incorporated by reference herein in their entireties, including the drawings.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60171728 |
Dec 1999 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09597604 |
Jun 2000 |
US |
Child |
10844874 |
May 2004 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08969437 |
Nov 1997 |
US |
Child |
09597604 |
Jun 2000 |
US |