Claims
- 1. A method of detecting cartilage disease, comprising incubating a sample containing an analyte with an immunological reagent that binds native melanoma inhibiting activity protein to form a complex of the analyte and the reagent and detecting said complex as an indication of the presence of cartilage disease, wherein detecting melanoma inhibiting activity protein at a level of 6.5 ng/ml or above is indication of disease.
- 2. The method according to claim 1 wherein said immunological reagent is at least one antibody that binds native melanoma inhibiting activity protein.
- 3. The method according to claim 2 wherein said at least one antibody comprises a monoclonal antibody that binds native melanoma inhibiting activity protein.
- 4. The method according to claim 1 wherein said immunological reagent includes a antibody that binds native melanoma inhibiting activity protein and that binds to a solid phase.
- 5. The method according to claim 4, further including a antibody having a label and binding at least one of said antibody that binds native melanoma inhibiting activity protein and a melanoma inhibiting activity protein analyte.
- 6. The method according to claim 4 wherein said analyte is detected using a sandwich test.
- 7. The method according to claim 1 wherein said analyte is detected using an ELISA test.
- 8. The method according to claim 1 wherein said immunological test reagent is applied to a test strip.
- 9. The method according to claim 1 wherein the disease detected is a degenerative cartilage disease.
- 10. The method according to claim 1 wherein the disease detected is arthritis.
- 11. The method according to claim 1 wherein the disease detected is rheumatoid arthritis, osteoarthritis, psoriases arthritis, HLA B27, gout, systemic scleroses, systemic lupus erythematodes or relapsing polychondritis.
- 12. The method according to claim 10 wherein the disease detected is rheumatoid arthritis.
Parent Case Info
This application claims the benefit of U.S. Provisional Application Ser. No. 60/062,234, filed Oct. 16, 1997.
Non-Patent Literature Citations (2)
Entry |
Bosserhoff, et al.: Melanoma-inhibiting activity . . . : Can. Res.: 57: pp. 3149-3153, Aug. 1997. |
Muller-ladner, et al.: MIA (melanoma inhibiting protein) . . . : Arth. Rheu.: 40 (9 Suppl.): 715 abstract, Sep. 1997. |