This invention relates to compositions comprising certain lipase variants and a photobleach and processes for making and using such compositions. Including the use of such compositions to clean and/or treat a situs.
Description
BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 shows the alignment of lipases.
Claims
1. A composition comprising a photobleach and a variant of a parent lipase, said variant, when compared to said parent, comprising a total of at least three substitutions, said substitutions being selected from one or more of the following groups of substitutions:
a.) at least two substitutions in Region I,b) at least one substitution in Region II,c) at least one substitution in Region III, and/ord) at least one substitution in Region IV.
2. A detergent composition according to claim 1, wherein said substitutions in Region I comprise substitutions in the positions corresponding to the positions 231 and 233.
3. A detergent composition according to claim 2 wherein said substitutions at positions 231 and 233 are substituted with an R.
4. A detergent composition according to claim 2, wherein said variant comprises a substitution in the position corresponding to position 4 of SEQ ID NO:2.
5. A detergent composition according to claim 4, wherein said substitution in the position corresponding to position 4 of SEQ ID NO:2 is V.
6. A detergent composition according to claim 2, wherein said variant comprises a substitution in the corresponding to position 227 of SEQ ID NO:2.
7. A detergent composition according to claim 6, wherein said substitution in the position corresponding to position to position 227 of SEQ ID NO:2 is G.
8. A detergent composition according to claim 1, wherein said at least one substitution in Region II comprises a substitution selected from the group consisting of substitutions in positions corresponding to the positions 202, 211, 255 and 256.
9. A detergent composition according to claim 8, wherein said at least one substitution in Region II comprises a substitution selected from the group consisting of X202G, X211L, X255Y/V and X256K.
10. A detergent composition according to claim 1, wherein said at least one substitution in Region II comprises a substitution in the position corresponding to the position 210.
11. A detergent composition according to claim 10, wherein said substitution in the position corresponding to the position 210 comprises X210K.
12. A detergent composition according to claim 1, wherein said at least one substitution in Region III comprises a substitution selected from the group consisting of substitutions in positions corresponding to the positions 86 and 90.
13. A detergent composition according to claim 12, wherein said at least one substitution in Region III comprises a substitution selected from the group consisting of X86V and X90A/R.
14. A detergent composition according to claim 1, wherein said at least one substitution in Region III comprises a substitution in the position corresponding to the position 83.
15. A detergent composition according to claim 14, wherein said substitution in the position corresponding to the position 83 comprises X83T.
16. A detergent composition according to claim 1, wherein said at least one substitution in Region IV comprises a substitution selected from the group consisting of substitutions in positions corresponding to the positions 27, 58 and 60.
17. A detergent composition according to claim 15, wherein said at least one substitution in Region IV comprises a substitution selected from the group consisting of X27R, X58N/A/G/P/T and X60S/V/G/N/R/K/A/L.
18. A detergent composition according to claim 1, comprising at least two substitutions in Region IV corresponding to the positions 27, 58 and 60.
19. A detergent composition according to claim 1, comprising at least two substitutions in Region IV selected from the group consisting of X27R, X58N/A/G/P/T and X60S/V/G/N/R/K/A/L.
20. A detergent composition according to claim 1, wherein said variant comprises at least one substitution outside the defined Regions I to IV.
21. A detergent composition according to claim 20, wherein said at least one substitution outside the defined Regions I to IV is selected from the group consisting of substitutions in positions corresponding to position 81, 147, 150 and 249.
22. A detergent composition according to claim 20, wherein said at least one substitution outside the defined Regions I to IV is selected from the group consisting of X81Q/E, X147M/Y, X150G and X249R/I/L.
23. A detergent composition according to claim 2, wherein said parent lipase is at least 90% identical to SEQ ID NO:2.
24. A detergent composition according to claim 1 wherein the parent lipase is identical to SEQ ID NO: 2 and said variant comprises one of the following groups of substitutions:
a) T231R+N233R+I255Yb) I202G+T231R+N233Rc) I86V+L227G+T231R+N233R+P256Kd) Q4V+S58N+V60S+T231R+N233Re) S58N+V60S+I90R+T231R+N233Rf) I90A+T231R+N233R+I255Vg) S58N+V60S+I86V+A150G+L227G+T231R+N233R+P256Kh) S58N+V60S+L147M+F211L+T231R+N233Ri) Q4V+S58A+V60S+S83T+I86V+A150G+E210K+L227G+T231R+N233R+P256Kj) S58N+V60S+I86V+A150G+L227G+T231R+N233R+P256K.
25. A detergent composition according to claim 1 wherein the parent lipase is identical to SEQ ID NO: 2 and said variant comprises one of the following groups of substitutions:
a) Q4V+S58A+V60S+S83T+I86V+A150G+E210K+L227G+T231R+N233R+P256Kb) S58N+V60S+I86V+A150G+L227G+T231R+N233R+P256K.
26. A detergent composition according to claim 1 wherein the lipase variant is characterized in that the Benefit Risk, when measured as given in the specification, is larger than 1.
27. A detergent composition comprising a photobleach and a polypeptide having lipase activity and which further has a Average Relative Performance of at least 0.8 and a Benefit Risk of at least 1.1 at the test conditions given in the specification.
28. A composition according to claim 1 comprising 0.1 to 40% anionic surfactant.
29. A composition according to claim 28, said composition being a cleaning and/or treatment composition.
30. A composition according to claim 1, said composition comprising sulfonated zinc phthalocyanine.
31. A composition according to claim 25 comprising a mixture of sulfonated zinc phthalocyanine and sulfonated aluminium phthalocyanine, said mixture having a weight ratio of sulfonated zinc phthalocyanine to sulfonated aluminium phthalocyanine greater than 1.
32. A composition according to claim 1, said composition comprising sulfonated aluminium phthalocyanine.
33. A composition according to claim 1 wherein the photobleach comprises a xanthene dye, anthraquinone or naphthaquinone.
34. A process of cleaning and/or treating a surface or fabric comprising the steps of optionally washing and/or rinsing said surface or fabric, contacting said surface or fabric with the composition of claim 1, then optionally washing and/or rinsing said surface or fabric.
35. A composition according to claim 1, wherein said lipase variant is a variant of SEQ ID NO: 2 comprising at least one of the mutations Q4V, S58N/A/G/P/T, I90R or Q249I/L.
Patent Application
20070191247
