Patent Application
20240317736
The present invention belongs to the field of medicine, and particularly relates to a deuterated derivative as an ATX inhibitor, or a stereoisomer, solvate, prodrug, metabolite, pharmaceutically acceptable salt or eutectic thereof, and the use thereof in the preparation of a drug for treating a related disease.
Autotaxin (ATX) is an extracellular secretase (also known as ENPP2), and its main physiological function is to produce lysophosphatidic acid (LPA) and choline having biological activities by means of hydrolysing lysophosphatidylcholine (LPC). Hydrolysis of LPC by ATX is the major source of LPA in blood. By acting on LPA receptors (at least 6 kinds of LPA1-6), LPA produces a series of physiological activities including cell proliferation, survival, movement, etc. ATX-LPA signalling pathways are involved in many pathological processes, including angiogenesis, autoimmune diseases, inflammation, fibrosis, neurodegenerative diseases and pain. The most widely studied pathological processes are fibrosis and tumour, particularly idiopathic pulmonary fibrosis (IPF). In 2014, FDA approved two new drugs Pirfenidone and Nintedanib (a triple angiokinase inhibitor), which are used for treating IPF, primarily for preventing further development and deterioration of IPF, but exhibit less significant curative effect on IPF itself. Therefore, people have been committed to finding more effective drugs for IPF treatment. GLPG-1690 as an ATX inhibitor has a relatively fast clinical progress (phase III clinical trial) for the treatment of IPF, and has shown a good curative effect in a phase II clinical trial.
An object of the present invention is to provide a deuterated ATX inhibitor, which has the advantages of novel structure, good efficacy, high bioavailability, less side effects, rapid onset and long acting.
The present invention relates to a compound of formula (I) or a stereoisomer, solvate, prodrug, metabolite, pharmaceutically acceptable salt or eutectic thereof, wherein
In certain embodiments, R22 is selected from H;
The present invention provides the compound of formula (I) or the stereoisomer, solvate, prodrug, metabolite, pharmaceutically acceptable salt or eutectic thereof, wherein the compound has a structure selected from one of:
More specifically, the present invention further provides a crystal form I of the compound of formula (A). The crystal form has high purity, low hygroscopicity, good solubility, capabilities of resisting high temperature, high humidity and strong light, high stability, an appropriate particle size, easiness to filter and good fluidity, and is suitable for the preparation of drug dosage forms.
The crystal form I of the compound of formula (A) has an X-ray powder diffraction pattern comprising characteristic diffraction peaks at 9.35±0.2°, 10.32±0.2°, 12.08±0.2° and 15.10±0.2° 2θ, as determined by using Cu-Kα radiation.
Furthermore, the crystal form I of the compound of formula (A) has an X-ray powder diffraction pattern further comprising characteristic diffraction peaks at 17.29±0.2°, 18.26±0.2° and 20.58±0.2° 2θ.
Furthermore, the crystal form I of the compound of formula (A) has an X-ray powder diffraction pattern further comprising characteristic diffraction peaks at 6.92±0.2°, 7.23±0.2°, 13.46±0.2°, 19.47±0.2° and 24.85±0.2° 2θ.
Furthermore, the crystal form I of the compound of formula (A) has an X-ray powder diffraction pattern substantially as shown in
Furthermore, the crystal form I of the compound of formula (A) has an X-ray powder diffraction peak substantially as shown in Table 1.
Furthermore, the crystal form I of the compound of formula (A) has a TGA curve substantially as shown in
Furthermore, with regard to the crystal form I of the compound of formula (A) of the present invention, the crystal has a particle size less than 100 μm; in some embodiments, the crystal has a particle size less than 90 μm; in some embodiments, the crystal has a particle size less than 80 μm; in some embodiments, the crystal has a particle size less than 70 μm; in some embodiments, the crystal has a particle size less than 60 μm; and in some embodiments, the crystal has a particle size less than 50 μm.
The present invention further provides a method for preparing a crystal form I of a compound of formula (A), wherein the method comprises: adding a crude product of the compound of formula (A) to an alcohol solvent, warming and stirring to dissolve same, then cooling to room temperature, and performing crystallization and filtration to obtain the crystal form I.
In some embodiments, according to the above-mentioned preparation method, the alcohol solvent is selected from methanol, ethanol, propyl alcohol, isopropyl alcohol or a combination thereof.
In some embodiments, according to the above-mentioned preparation method, the ratio of the compound of formula (A) to the alcohol solvent is 1:5-8 g/mL. In some embodiments, the ratio of the compound of formula (A) to the alcohol solvent is 1:5-6 g/mL.
In some embodiments, according to the above-mentioned preparation method, the warming involves warming to 60° C.-90° C. In some embodiments, the warming involves warming to 70° C.-80° C. In some embodiments, the warming involves warming to 75° C.
The crystal form I of the compound of formula (A) of the present invention accounts for about 5 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 10 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 15 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 20 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 25 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 30 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 35 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 40 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 45 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 50 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 55 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 60 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 65 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 70 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 75 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 80 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 85 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 90 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 95 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 98 wt % to about 100 wt % of a bulk drug; in certain embodiments, the crystal form I of the compound of formula (A) accounts for about 99 wt % to about 100 wt % of a bulk drug; and in certain embodiments, a bulk drug is basically composed of the crystal form I of the compound of formula (A) of the present invention, that is, the bulk drug is basically a phase-pure crystal.
The present invention further provides compounds of formula (II-A), formula (II-B) and formula (II-C) as shown below:
The compounds of formula (II-A), formula (II-B) and formula (II-C) are used as intermediates of the compound of formula (A).
The present invention further provides a method for preparing a compound of formula (A), wherein the method comprises the following steps:
In some embodiments, the above-mentioned preparation method involves dissolving a compound of formula (II-C) in an organic solvent A, and reacting the resulting solution with 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone and a base preferably at 72° C.-88° C. for 3-10 h, further preferably at 75° C.-85° C. for 4-8 h, and more preferably at 80° C. for 6 h.
In some embodiments, according to the above-mentioned preparation method, the organic solvent A is selected from at least one of acetonitrile, 2-methyltetrahydrofuran, ethylbenzene, methyl and ethyl glyoxylate and diethylene glycol tert-butyl ether.
In some embodiments, according to the above-mentioned preparation method, the organic solvent A is selected from acetonitrile.
In some embodiments, according to the above-mentioned preparation method, the base is at least one of potassium carbonate and sodium carbonate.
In some embodiments, according to the above-mentioned preparation method, the base is potassium carbonate.
Further, the method for preparing the compound of formula (A) further comprises the following steps:
Further, the method for preparing the compound of formula (A) further comprises the following steps:
Further, the method for preparing the compound of formula (A) further comprises the following steps:
The method for preparing the compound of formula (A) of the present invention further comprises the following steps:
The method for preparing the compound of formula (A) of the present invention comprises the following steps:
Further, according to the above-mentioned preparation method, the aprotic polar solvent is at least one of N,N-dimethylacetamide, dimethylformamide, hexamethylphosphoramide, acetonitrile, acetone and dimethyl sulfoxide.
Furthermore, the aprotic polar solvent is N,N-dimethylacetamide.
Further, according to the above-mentioned preparation method, the organic solvent B is at least one of tetrahydrofuran, N,N-dimethylacetamide, dimethylformamide, acetonitrile and acetone.
Furthermore, the organic solvent B is tetrahydrofuran.
Further, according to the above-mentioned preparation method, the reducing agent is sodium hydride.
Further, according to the above-mentioned preparation method, the organic solvent C is at least one of dichloromethane, methyl acetate, dimethyl carbonate, propylene glycol methyl ether acetate and dimethyl nylon acid.
Furthermore, the organic solvent C is dichloromethane.
As another technical solution of the present invention, the present invention provides a pharmaceutical composition comprising the compound or the stereoisomer, solvate, prodrug, metabolite, pharmaceutically acceptable salt or eutectic thereof according to any one of the above-mentioned technical solutions, or the crystal form I of the compound of formula (A), and a pharmaceutically acceptable carrier and/or excipient.
As yet another technical solution of the present invention, the present invention provides the use of the compound or the stereoisomer, solvate, prodrug, metabolite, pharmaceutically acceptable salt or eutectic thereof according to any one of the above-mentioned technical solutions or the above-mentioned composition in the preparation of a drug for treating an ATX-mediated disease. The ATX-mediated disease is idiopathic pulmonary fibrosis.
It can be understood that, as is well known in the fields of thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), melting peak heights of a TGA curve and a DSC curve depend on many factors related to sample preparation and geometric shapes of instruments, while peak positions are relatively insensitive to experiment details. Therefore, in some embodiments, the crystallized compounds of the present invention have TGA and DSC patterns comprising characteristic peak positions, which have substantially the same properties as the TGA and DSC patterns provided in the drawings of the present invention, with an error tolerance of measured values within ±5° C., which is generally required to be within ±3° C.
It can be understood that the numerical values described and claimed in the present invention are approximate values. Changes in values may be attributed to device calibration, device errors, crystal purity, crystal size, sample size and other factors.
It can be understood that the crystal forms of the present invention are not limited to the characteristic patterns such as XRD, DSC, TGA, DVS and adsorption isotherm curve graphs which are completely identical to those described in the drawings disclosed by the present invention, and any crystal form having a characteristic pattern which is essentially or substantially the same as those described in the drawings falls within the scope of the present invention.
The term “therapeutically effective amount” means an amount that causes a physiological or medical response in a tissue, system or subject and is a desirable amount, including the amount of a compound that is, when administered to a subject to be treated, sufficient to prevent occurrence of one or more symptoms of the disease or condition to be treated or to reduce the symptom(s) to a certain degree.
The term “room temperature” refers to: 10° C.-30° C., >30% RH.
The term “pharmaceutically acceptable salt” refers to a salt of the compound of the present invention, which salt maintains the biological effectiveness and characteristics of a free acid or a free base and is obtained by reacting the free acid with a non-toxic inorganic base or organic base, or reacting the free base with a non-toxic inorganic acid or organic acid.
The term “pharmaceutical composition” represents a mixture of one or more compounds or the stereoisomers, solvates, pharmaceutically acceptable salts, eutectics or deuterated products thereof of the present invention and other components comprising physiologically/pharmaceutically acceptable carriers and/or excipients.
The term “carrier” refers to a system that does not cause significant irritation to an organism and does not eliminate the biological activities and characteristics of the administered compound, and can change the way in which the drug enters the human body and the distribution of the drug in the body, control the release rate of the drug and delivery the drug to target organs. Non-limiting examples of carriers include a microcapsule, a microsphere, a nanoparticle, a liposome, etc.
The term “excipient” refers to a substance that is not a therapeutic agent per se, but used as a diluent, adjuvant, adhesive and/or vehicle for addition to a pharmaceutical composition, thereby improving the disposal or storage properties thereof, or allowing to or promoting the formation of a compound or a pharmaceutical composition into a unit dosage form for administration. As is known to a person skilled in the art, a pharmaceutically acceptable excipient can provide various functions and can be described as a wetting agent, a buffer, a suspending aid, a lubricant, an emulsifier, a disintegrating agent, an absorbent, a preservative, a surfactant, a colourant, a flavouring agent and a sweetening agent. Examples of pharmaceutically acceptable excipients include, but are not limited to: (1) sugars, such as lactose, glucose and sucrose; (2) starch, such as corn starch and potato starch; (3) cellulose and derivatives thereof, such as sodium carboxymethyl cellulose, ethyl cellulose, cellulose acetate, hydroxypropyl methylcellulose, hydroxypropyl cellulose, microcrystalline cellulose and croscarmellose (such as croscarmellose sodium); (4) tragacanth powder; (5) malt; (6) gelatine; (7) talc; (8) excipients, such as cocoa butter or suppository wax; (9) oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; (10) diols, such as propylene glycol; (11) polyols, such as glycerol, sorbitol, mannitol and polyethylene glycol; (12) esters, such as ethyl oleate and ethyl laurate; (13) agar; (14) buffers, such as magnesium hydroxide and aluminium hydroxide; (15) alginic acid; (16) pyrogen-free water; (17) isotonic saline; (18) Ringer's solution; (19) ethanol; (20) pH buffered solution; (21) polyester, polycarbonate and/or polyanhydride; and (22) other non-toxic compatible substances used in a pharmaceutical preparation.
The term “stereoisomer” refers to an isomer produced as a result of different spatial arrangement of atoms in molecules, including cis-trans isomers, enantiomers and conformational isomers.
The term “solvate” refers to a substance formed by the compound of the present invention or the salt thereof and a stoichiometric or non-stoichiometric solvent bound by intermolecular non-covalent forces. When the solvent is water, the solvate is a hydrate.
The term “eutectic” refers to a crystal formed by the combination of an active pharmaceutical ingredient (API) and a co-crystal former (CCF) under the action of hydrogen bonds or other non-covalent bonds, wherein the pure states of API and CCF are both solid at room temperature, and there is a fixed stoichiometric ratio between the components. The eutectic is a multi-component crystal, which includes both a binary eutectic formed by means of two neutral solids and a multi-element eutectic formed by means of a neutral solid and a salt or solvate.
The content of the present invention is described in detail with reference to the following examples. If a specific condition is not indicated in the examples, a conventional condition is used in an experimental method. The listed examples are intended to better illustrate the content of the present invention, but should not be construed as limiting the content of the present invention. According to the above-mentioned content of the invention, a person skilled in the art can make unsubstantial modifications and adjustments to the embodiments, which still fall within the scope of protection of the present invention.
Unless otherwise specified, the raw materials are purchased from Titan Technology Co., Ltd., Energy Chemical Co., Ltd., Shanghai Demo Co., Ltd., Chengdu Kelong Chemical Co., Ltd., Accela ChemBio Co., Ltd., PharmaBlock Sciences (Nanjing), Inc., WuXi Apptec Co., Ltd., J&K Scientific Co., Ltd., etc.
The structures of the compounds are determined by nuclear magnetic resonance (NMR) or (and) mass spectrometry (MS). The NMR shift (δ) is given in the unit of 10-6 (ppm). NMR is determined with Bruker Avance III 400 and Bruker Avance 300; the solvent for determination is deuterated dimethyl sulfoxide (DMSO-d6), deuterated chloroform (CDCl3) and deuterated methanol (CD3OD); and the internal standard is tetramethylsilane (TMS).
MS is measured with (Agilent 6120B (ESI) and Agilent 6120B (APCI));
HPLC determination is performed by using Agilent 1260DAD high pressure liquid chromatograph (Zorbax SB-C18 100×4.6 mm, 3.5 μM);
Yantai Huanghai HSGF254 or Qingdao GF254 silica gel plate is used as a silica plate for thin-layer chromatography, and the silica gel plate for the thin-layer chromatography (TLC) is of the specification of 0.15 mm-0.20 mm, and the specification used when a product is separated and purified product by means of thin-layer chromatography is 0.4 mm-0.5 mm.
For the column chromatography, 200-300 mesh Yantai Huanghai silica gel is generally used as a carrier.
An XRPD pattern is obtained on an X-ray powder diffractometer produced by PANalytacal, and scanning parameters are as shown in the table below.
TGA and DSC patterns are obtained on a TA Q5000/5500 thermal gravimetric analyzer and a TA 2500 differential scanning calorimeter, respectively, and test parameters are as shown in the table below.
A dynamic vapor sorption (DVS) curve is obtained on DVS Intrinsic of Surface Measurement Systems (SMS). The relative humidity at 25° C. is corrected by using the deliquescence points of LiCl, Mg(NO3)2 and KCl. Test parameters for DVS are as shown in the table below.
Data of polarized light microscope are collected by means of an Axio Lab. A1 upright microscope at room temperature.
Description of abbreviations:
Trimethylsilylacetylene (1a) (10.07 g, 102.5 mmol) was dissolved in dry tetrahydrofuran (45 mL) at room temperature and cooled to −78° C., and then n-butyllithium (45 mL, 2M n-hexane) was added dropwise. Upon completion of the dropwise addition, the resulting mixture was transferred to an ice-water bath, reacted for another 20 min, and then cooled to −78° C., and hexamethylphosphoramide (18.37 g, 102.5 mmol) was added dropwise and stirred for another 30 min under such condition. After that, ethyl-d5 iodide (1b) (15.00 g, 93.2 mmol) was added dropwise. Upon completion of the dropwise addition, the resulting mixture was naturally warmed to room temperature and reacted overnight. The reaction was quenched by adding water (20 mL) to the reaction liquid. Liquid separation was performed. The aqueous phase was extracted with ethyl ether (20 mL), and the organic phases were combined, washed with saturated brine (20 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was distilled under normal pressure, and the fraction at 80° C. to 100° C. was collected to obtain the title compound (but-1-yn-1-yl-d5)trimethylsilane (intermediate 1) as a colourless transparent liquid (10.0 g, yield: 74%).
1-Boc-3-azetidinone (2a) (5.13 g, 30 mmol) was dissolved in tetrahydrofuran (50 mL) and stirred uniformly. Sodium borohydride-d4 (1.26 g, 30 mmol) was then added in portions, and the resulting mixture was reacted at room temperature for 1 h. The reaction was quenched by adding water (20 mL) and stirred for 10 min. Ethyl acetate (100 mL) and saturated sodium bicarbonate (100 mL) were then added, and extraction and layer separation were performed. The organic phases were washed with saturated brine (100 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound tert-butyl 3-hydroxyazetidine-1-carboxylate-3-d (2b) as a transparent oil (4.6 g, yield: 87%).
1H NMR (400 MHZ, CDCl3) δ 4.14 (d, 2H), 3.79 (d, 2H), 1.44 (s, 9H).
Step 2:
The compound tert-butyl 3-hydroxyazetidine-1-carboxylate-3-d (2b) (4.6 g, 26.3 mmol) was dissolved in dichloromethane (24 mL), and trifluoroacetic acid (8 mL) was added dropwise. Upon completion of the dropwise addition, the resulting mixture was reacted at room temperature for 1 h and concentrated under reduced pressure to obtain a trifluoroacetate of azetidin-3-d-3-ol (2c) as a brown oil (4.94 g, yield: 100%), and the crude product was directly used in the next reaction.
Step 3:
The trifluoroacetate (4.94 g, 26.3 mmol) of azetidin-3-d-3-ol (2c) was dissolved in water (40 mL), and stirred uniformly. Potassium carbonate (12.4 g, 60 mmol) was then added in portions, and after the system stopped bubbling, dichloromethane (40 mL) was added. Chloroacetyl chloride (3.39 g, 30 mmol) was added dropwise under an ice bath condition. Upon completion of the dropwise addition, the resulting mixture was reacted at room temperature for 2 h. Ethyl acetate (100 mL) and water (100 mL) were added, and extraction and layer separation were performed. The aqueous phase was extracted with ethyl acetate (100 mL), and the organic phases were combined, washed with saturated brine (100 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel to obtain the target compound 2-chloro-1-(3-hydroxyazetidin-1-yl-3-d)ethan-1-one (intermediate 2) as a white solid (0.85 g, yield: 21%).
1H NMR (400 MHZ, CDCl3) δ 4.49 (d, 1H), 4.30 (d, 1H), 4.15 (d, 1H), 3.94 (d, 1H), 3.90 (s, 2H), 3.01 (s, 1H).
Acetic acid-d4 (3a) (1.0 g, 15.6 mmol) was dissolved in trifluoroacetic anhydride (6 mL) and stirred uniformly. After that, 4-dimethylaminopyridine (30 mg, 0.25 mmol) was added and heated to 60° C., and bromine (0.98 mL) was slowly added dropwise under such condition for 1 h. Upon completion of the dropwise addition, the resulting mixture was reacted under such condition for 1 h and then cooled to room temperature, and nitrogen gas was introduced until the volume of the system was reduced to about 1 mL. Ethanol (20 mL) was added and reacted at 60° C. overnight. The resultant was then cooled to room temperature, and concentrated under reduced pressure. Ethyl ether (100 mL) was added, and the resulting mixture was washed with saturated brine (100 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain ethyl 2-bromoacetate-d2 (intermediate 3) as a pale yellow liquid (1.65 g, yield: 62.6%).
1H NMR (400 MHZ, CDCl3) δ 4.24 (q, J=7.1 Hz, 1H), 1.30 (t, J=7.1 Hz, 2H).
Step 1:
4-fluorobenzoic acid-2,3,5,6-d4 (4a) (1.0 g, 6.94 mmol) was dissolved in N,N-dimethylformamide (10 mL) and cooled to 0° C. Potassium carbonate (1.92 g, 13.9 mmol) was added and reacted under such condition for 5 min. Dimethyl sulphate (1.05 g, 8.33 mmol) was then added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 1 h. The reaction was quenched by adding water (20 mL) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (petroleum ether:ethyl acetate (v/v)=8:1) to obtain the target compound methyl 4-fluorobenzoate-2,3,5,6-d4 (4b) as a transparent liquid (0.96 g, yield: 87%).
1H NMR (400 MHZ, CDCl3) δ 3.91 (s, 3H).
Step 2:
Methyl 4-fluorobenzoate-2,3,5,6-d4 (4b) (0.96 g, 6.1 mmol) was dissolved in methylbenzene (8 mL) and cooled to-10° C. under nitrogen protection. Acetonitrile (1.46 g, 36.3 mmol) was added, and sodium bis(trimethylsilyl)amide (6 mL, 12 mmol, 2M in THF) was slowly added dropwise. Upon completion of the dropwise addition, the reaction was quenched with hydrochloric acid (20 mL, 1N aqueous solution). The reaction product was warmed to room temperature and extracted with ethyl acetate (50 mL×2). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound 3-(4-fluorophenyl-2,3,5,6-d4)-3-oxopropanenitrile (4c) as a pale yellow solid (0.92 g, yield: 91%).
1H NMR (400 MHZ, CDCl3) δ 4.05 (s, 2H). Step 3:
3-(4-fluorophenyl-2,3,5,6-d4)-3-oxopropanenitrile (4c) (0.92 g, 5.5 mmol) was dissolved in ethanol (12 mL). Pyridine (0.44 g, 5.5 mmol) was added, and then the resulting mixture was warmed to 70° C., reacted for 15 min and cooled to room temperature, and a suspension (8 mL) of thiourea (0.84 g, 11 mmol) and iodine (1.4 g, 5.5 mmol) in ethanol was slowly added dropwise. Upon completion of the dropwise addition, the resulting mixture was reacted at room temperature for 1 h. The reaction was quenched by adding sodium thiosulphate (10 mL, 1N aqueous solution) and filtered. The filter cake was dried to obtain the target compound 2-amino-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (4d) as a white solid (0.82 g, yield: 67%).
Step 4:
Copper chloride (0.60 g, 4.5 mmol) was dissolved in acetonitrile (8 mL), tert-butyl nitrite (0.67 g, 5.6 mmol) was added dropwise, and the mixture was stirred at room temperature for 30 min. After that, 2-amino-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (4d) (0.82 g, 3.67 mmol) was added in portions. Upon completion of the addition, the resulting mixture was reacted at room temperature for another 1 h. The reaction was quenched with hydrochloric acid (10 mL, 1N aqueous solution). Ethyl acetate (80 mL) was added, and the resulting mixture was washed with saturated brine (50 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane) to obtain the target compound 2-chloro-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (intermediate 4) as a pale yellow solid (0.68 g, yield: 76%).
Step 1:
NMP (220 mL) was added to a 500 mL reaction flask and stirred, and then 2-chloro-5-fluoro-4-iodopyridine (1A) (44.00 g, 170.9 mmol), 1-Boc-piperazine (1B) (47.75 g, 256.4 mmol) and potassium carbonate (47.24 g, 341.8 mmol) were added successively. The resulting mixture was warmed to 150° C. and reacted for 3 h, and then the reaction stopped. The reaction liquid was cooled to room temperature, the reactant was slowly added to ice water (500 mL), and a white solid was precipitated out. Stirring was performed for 0.5 h, and then filtration was performed. The filter cake was washed with water, slurried with n-hexane (200 mL), filtered and dried to obtain the target compound tert-butyl 4-(2-chloro-5-fluoropyridin-4-yl)piperazine-1-carboxylate (1C) as a white solid (50 g, yield: 64%).
LCMS m/z=316.2[M+1]+.
Step 2:
Tert-butyl 4-(2-chloro-5-fluoropyridin-4-yl)piperazine-1-carboxylate (1C) (23.18 g, 71.6 mmo), (but-1-yn-1-yl-d5)trimethylsilane (intermediate 1) (9.4 g, 131.3 mmol), bistriphenylphosphine palladium dichloride (5.03 g, 7.2 mmol), 1,3-bis(diphenylphosphino)propane (4.43 g, 10.7 mmol) and caesium fluoride (22.8 g, 143.3 mmol) were successively added to dimethyl sulfoxide (184 mL). The system was subjected to nitrogen replacement three times and reacted at 95° C. for 4 h. After the reaction was completed, the reaction product was cooled to room temperature, and water (200 mL) was added. The aqueous phase was extracted with ethyl acetate (200 mL×3). The organic phases were combined, washed with a saturated aqueous sodium chloride solution (200 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure. The residue was separated by column chromatography on silica gel (PE:EA=20:1-5:1) to obtain tert-butyl 4-(2-(but-1-yn-1-yl-d5)-5-fluoropyridin-4-yl)piperazine-1-carboxylate (1D) as a pale brown viscous liquid (10.15 g, yield: 41.9%).
LCMS m/z=339.2[M+1]+.
Step 3:
Ethanol (100 mL) and tert-butyl 4-(2-(but-1-yn-1-yl-d5)-5-fluoropyridin-4-yl)piperazine-1-carboxylate (1D) (10.15 g, 30.0 mmol) were added to a 250 mL reaction flask and cooled to 0° C. 2-[(aminooxy)sulfonyl]-1,3,5-trimethylbenzene (1E) (11.00 g, 51.1 mmol) was added in portions, and then sodium bicarbonate (5.04 g, 60.0 mmol) was added to the reaction. Upon completion of the addition, the mixture was reacted at room temperature for 2 h. After that, potassium carbonate (8.29 g, 60.0 mmol) was added to the reaction and reacted at room temperature overnight. Water (150 mL) was added to the reaction liquid, and the aqueous phase was extracted with ethyl acetate (200 mL×2). The organic phases were combined, washed with saturated sodium chloride (100 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated and purified by column chromatography on silica gel to obtain the target compound tert-butyl 4-(2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1F) as a white solid (7.53 g, yield: 71%).
LCMS m/z=354.3 [M+1]+.
Step 4:
Tert-butyl 4-(2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1F) (7.50 g, 21.2 mmol) was dissolved in methanol (37.5 mL) and acetic acid (7.5 mL), and the resulting solution was cooled to 5° C. After that, sodium nitrite (2.93 g, 42.4 mmol) was dissolved in water (10 mL) and then added dropwise to the reaction liquid. Upon completion of the addition, the resulting mixture was reacted at room temperature for 16 h. Water (30 mL) was added dropwise to the reaction liquid. Upon completion of the addition, filtration was performed. The filter cake was washed with water (10 mL×2) and dried to obtain a crude product of the target compound tert-butyl 4-(2-(ethyl-d5)-6-fluoro-3-nitrosopyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1G) as a dark green solid (11.5 g).
LCMS m/z=383.3 [M+1]+.
Step 5:
Tert-butyl 4-(2-(ethyl-d5)-6-fluoro-3-nitrosopyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1G) (11.5 g, crude) was dissolved in ethanol (40 mL) and water (20 mL). After that, ammonium chloride (15.78 g, 295 mmol) and iron powder (8.23 g, 147 mmol) were added to the reaction, reacted at 65° C. for 20 min and filtered. The filtrate was extracted with dichloromethane (50 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound tert-butyl 4-(3-amino-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1H) as a yellow solid (7.85 g).
LCMS m/z=369.2[M+1]+.
Step 6:
Tert-butyl 4-(3-amino-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1H) (7.85 g, 21.3 mmol), 2-chloro-4-(4-fluorophenyl)thiazole-5-cyano (1I) (6.1 g, 25.6 mmol) (reference document: J. Med. Chem. 2017, 60, 3580-3590, DOI: 10.1021/acs.jmedchem.7b00032), 2,6-dimethylpyridine (3.42 g, 31.9 mmol) and N,N-dimethylacetamide (40 mL) were added. The resulting mixture was warmed to 70° C. and then reacted for 4 h. The reaction was quenched by adding a 10% sodium chloride solution (100 mL) and extracted with ethyl acetate (150 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1J) as a yellowish-brown viscous substance (15.50 g).
LCMS m/z=571.2[M+1]+.
Step 7:
The crude product (15.50 g) of tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1J) was dissolved in tetrahydrofuran (60 mL) and cooled to 0° C. under nitrogen protection. Sodium hydride (1.3 g, 32.6 mmol, 60 wt %) was added in portions. Upon completion of the addition, the mixture was reacted under such condition for 10 min, and then methyl iodide (4.63 g, 32.6 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (100 mL) and extracted with ethyl acetate (100 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1K) as a yellow solid (11.56 g, four-step yield: 93%).
Step 8:
The compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1K) (11.56 g, 19.8 mmol) was dissolved in dichloromethane (110 mL), and trifluoroacetic acid (35 mL) was added dropwise. The resulting mixture was reacted for about 3 h and then concentrated under reduced pressure. Dichloromethane (200 mL) was added to the residue, and the pH was adjusted to 8 with saturated sodium bicarbonate. Liquid separation was performed. The aqueous phase was extracted with dichloromethane (200 mL×2). The organic phases were combined, washed with saturated brine (300 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound 2-((2-(ethyl-d5)-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (1L) as a yellow solid (10.7 g).
Step 9:
2-((2-(ethyl-d5)-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (1L) (10.7 g) was dissolved in acetonitrile (100 mL), and 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone (1M) (3.63 g, 24.3 mmol, synthesized by the method in reference document: J. Med. Chem. 2017, 60, 3580-3590) and potassium carbonate (6.11 g, 44.2 mmol) were successively added. The resulting mixture was warmed to 80° C. and then reacted overnight. The reaction was quenched by adding water (100 mL) and extracted with ethyl acetate (150 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-(ethyl-d5)-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 1) (6.2 g, two-step yield: 53%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.18-8.10 (m, 2H), 7.20-7.11 (m, 2H), 6.45 (d, 1H), 4.67 (s, 1H), 4.50-4.40 (m, 1H), 4.32-4.23 (m, 1H), 4.15-4.07 (m, 1H), 3.93-3.85 (m, 1H), 3.58 (s, 3H), 3.30-3.20 m, 4H), 3.19-3.06 (m, 2H), 2.85-2.67 (m, 5H).
LCMS m/z=598.1[M+1]+.
Step 1:
The crude product (9.2 g, 16.1 mmol) of tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (1J) was dissolved in tetrahydrofuran (60 mL) and cooled to 0° C. under nitrogen protection. Sodium hydride (0.77 g, 19.3 mmol, 60 wt %) was added in portions. Upon completion of the addition, the resulting mixture was reacted under such condition for 10 min, and then methyl-d3 iodide (2.80 g, 19.3 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (100 mL) and extracted with ethyl acetate (100 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (2A) as a yellow solid (8.0 g, yield: 84.5%).
Step 2:
The compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-(ethyl-d5)-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (2A) (8.0 g, 13.6 mmol) was dissolved in dichloromethane (90 mL), and trifluoroacetic acid (30 mL) was added dropwise. The resulting mixture was reacted for about 3 h and then concentrated under reduced pressure. Dichloromethane (100 mL) was added to the residue, and the pH was adjusted to 8 with saturated sodium bicarbonate. Liquid separation was performed. The aqueous phase was extracted with dichloromethane (100 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound 2-((2-(ethyl-d5)-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (2B) as a yellow solid (6.40 g, yield: 96.3%).
Step 3:
2-((2-(ethyl-d5)-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (2B) (6.40 g, 13.1 mmol) was dissolved in acetonitrile (100 mL). 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone (1M) (2.16 g, 14.4 mmol) and potassium carbonate (3.62 g, 26.2 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 5 h. The reaction was quenched by adding water (60 mL) and extracted with ethyl acetate (100 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-(ethyl-d5)-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 2) (5.1 g, two-step yield: 64.8%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.20-8.11 (m, 2H), 7.20-7.11 (m, 2H), 6.45 (d, 1H), 4.67 (s, 1H), 4.50-4.40(m, 1H), 4.32-4.24 (m, 1H), 4.15-4.07 (m, 1H), 3.94-3.86 (m, 1H), 3.31-3.06 (m, 6H), 2.86-2.66 (m, 5H).
LCMS m/z=601.2[M+1]+.
Step 1:
Tert-butyl 4-(2-chloro-5-fluoropyridin-4-yl)piperazine-1-carboxylate (1C) (20.1 g, 62 mmo), (but-1-yn-1-yl)trimethylsilane (9.3 g, 74 mmol), bistriphenylphosphine palladium dichloride (4.4 g, 6.3 mmol), 1,3-bis(diphenylphosphino)propane (3.84 g, 9.3 mmol) and caesium fluoride (19.0 g, 125 mmol) were successively added to dimethyl sulfoxide (160 mL). The system was subjected to nitrogen replacement three times and reacted at 95° C. for 4 h. After the reaction was completed, the reaction product was cooled to room temperature, and water (160 mL) was added. The aqueous phase was extracted with ethyl acetate (200 mL×3). The organic phases were combined, washed with a saturated aqueous sodium chloride solution (200 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure. The residue was separated by column chromatography on silica gel (PE:EA=20:1-5:1) to obtain tert-butyl 4-(2-(but-1-yn-1-yl)-5-fluoropyridin-4-yl)piperazine-1-carboxylate (3A) as a pale brown viscous liquid (11.0 g, yield: 53%).
LCMS m/z=334.2[M+1]+.
Step 2:
Ethanol (100 mL) and tert-butyl 4-(2-(but-1-yn-1-yl)-5-fluoropyridin-4-yl)piperazine-1-carboxylate (3A) (11.0 g, 33 mmol) were added to a 250 mL reaction flask and cooled to 0° C. 2-[(aminooxy)sulfonyl]-1,3,5-trimethylbenzene (1E) (10.7 g, 49.5 mmol) was added in portions, and then sodium bicarbonate (5.5 g, 66 mmol) was added to the reaction. Upon completion of the addition, the mixture was reacted at room temperature for 2 h. After that, potassium carbonate (9.12 g, 66 mmol) was added to the reaction and reacted at room temperature overnight. Water (200 mL) was added to the reaction liquid, and the aqueous phase was extracted with ethyl acetate (200 mL×2). The organic phases were combined, washed with saturated sodium chloride (100 mL), dried over anhydrous sodium sulphate, filtered, concentrated and separated by column chromatography on silica gel to obtain the target compound tert-butyl 4-(2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3B) as a white solid (7.8 g, yield: 68%).
LCMS m/z=349.2[M+1]+.
Step 3:
Tert-butyl 4-(2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3B) (7.40 g, 22.4 mmol) was dissolved in methanol (40 mL) and acetic acid (8 mL), and the resulting solution was cooled to 5° C. After that, sodium nitrite (3.09 g, 44.8 mmol) was dissolved in water (10 mL) and slowly added dropwise to the reaction liquid. Upon completion of the addition, the resulting mixture was reacted at room temperature for 16 h. Water (30 mL) was added dropwise to the reaction liquid. Upon completion of the dropwise addition, filtration was performed. The filter cake was washed with water (10 mL×2) and dried to obtain a crude product of the target compound tert-butyl 4-(2-ethyl-6-fluoro-3-nitrosopyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3C) as a dark green solid (10.8 g).
LCMS m/z=378.3 [M+1]+.
Step 4:
Tert-butyl 4-(2-ethyl-6-fluoro-3-nitrosopyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3C) (10.8 g, crude) was dissolved in ethanol (40 mL) and water (20 mL). After that, ammonium chloride (14.98 g, 0.28 mol) and iron powder (7.84 g, 0.14 mol) were added to the reaction, reacted at 65° C. for 20 min and filtered. The filtrate was extracted with dichloromethane (60 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound tert-butyl 4-(3-amino-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3D) as a yellow solid (7.12 g, two-step yield: 87.5%).
LCMS m/z=364.2[M+1]+.
Step 5:
Tert-butyl 4-(3-amino-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3D) (7.12 g, 19.6 mmol), 2-chloro-4-(4-fluorophenyl)thiazole-5-cyano (1I) (5.61 g, 23.5 mmol), 2,6-dimethylpyridine (3.15 g, 29.4 mmol) and N,N-dimethylacetamide (40 mL) were added, and the resulting mixture was warmed to 70° C. and reacted for 5 h. The reaction was quenched by adding a 10% sodium chloride solution (100 mL) and extracted with ethyl acetate (150 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3E) as a yellowish-brown solid (10.0 g, yield: 90.3%).
LCMS m/z=566.2[M+1]+.
Step 6:
Tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3E) (10.0 g, 17.7 mmol) was dissolved in tetrahydrofuran (60 mL) and cooled to 0°° C. under nitrogen protection. Sodium hydride (0.85 g, 21.2 mmol, 60 wt %) was added in portions. Upon completion of the addition, the resulting mixture was reacted under such condition for 10 min, and then methyl-d3 iodide (3.07 g, 21.2 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (100 mL) and extracted with ethyl acetate (100 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3F) as a yellow solid (11.7 g).
LCMS m/z=583.2[M+1]+.
Step 7:
The crude product (11.7 g) of the compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3F) was dissolved in dichloromethane (100 mL), and trifluoroacetic acid (33 mL) was added dropwise. The resulting mixture was reacted for about 3 h and then concentrated under reduced pressure. Dichloromethane (200 mL) was added to the residue, and the pH was adjusted to 8 with saturated sodium bicarbonate. Liquid separation was performed. The aqueous phase was extracted with dichloromethane (200 mL×2). The organic phases were combined, washed with saturated brine (300 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound 2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (3G) as a yellow solid (10.0 g).
LCMS m/z=483.2[M+1]+.
Step 8:
The crude product (10.0 g) of 2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (3G) was dissolved in acetonitrile (100 mL). 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone (1M) (3.35 g, 22.4 mmol) and potassium carbonate (5.55 g, 40.1 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 6 h. The reaction was quenched by adding water (100 mL) and extracted with ethyl acetate (150 mL×3). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 3) (7.6 g, three-step yield: 72.1%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.20-8.10 (m, 2H), 7.20-7.11 (m, 2H), 6.45 (d, 1H), 4.67 (s, 1H), 4.50-4.40 (m, 1H), 4.32-4.24 (m, 1H), 4.16-4.08 (m, 1H), 3.94-3.86 (m, 1H), 3.29-3.07 (m, 6H), 2.78(s, 4H), 2.72 (q, 2H), 2.58 (s, 1H), 1.32 (t, 3H).
LCMS m/z=596.3[M+1]+.
Step 1:
To a 250 mL reaction flask, NMP (93 mL) was added, and then 2-chloro-5-fluoro-4-iodopyridine (1A) (9.3 g, 36.2 mmol), piperazin-1-tert-butyl carbonate-2,2,3,3,5,5,6,6-d8 (4A) (4.5 g, 24.2 mmol) and potassium carbonate (6.7 g, 48.3 mmol) were added successively. The resulting mixture was warmed to 150° C. and reacted for 3 h, and then the reaction stopped. The reaction liquid was cooled to room temperature, the reactant was slowly added to ice water (200 mL), and a white solid was precipitated out. Stirring was performed for 0.5 h, and then filtration was performed. The filter cake was washed with water, slurried with n-hexane (100 mL), filtered and dried to obtain the target compound tert-butyl 4-(2-chloro-5-fluoropyridin-4-yl)piperazine-1-carboxylate-2,2,3,3,5,5,6,6-d8 (4B) as a white solid (10.01 g, yield: 85%).
LCMS m/z=324.3[M+1]+.
Step 2:
Tert-butyl 4-(2-chloro-5-fluoropyridin-4-yl)piperazine-1-carboxylate (4B) (2.00 g, 6.2 mmol), (but-1-yn-1-yl)trimethylsilane (0.94 g, 7.4 mmol), bistriphenylphosphine palladium dichloride (0.44 g, 0.6 mmol), 1,3-bis(diphenylphosphino)propane (0.38 g, 0.9 mmol) and caesium fluoride (1.89 g, 12.4 mmol) were successively added to dimethyl sulfoxide (16 mL). The system was subjected to nitrogen replacement three times and reacted at 95° C. for 5 h. After the reaction was completed, the reaction product was cooled to room temperature, and water (20 mL) was added. The aqueous phase was extracted with ethyl acetate (20 mL×3). The organic phases were combined, washed with a saturated aqueous sodium chloride solution (20 mL), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure. The residue was separated by column chromatography on silica gel (PE:EA=15:1-5:1) to obtain 4C (1.20 g, yield: 57%).
LCMS m/z=342.3[M+1]+.
Step 3:
Ethanol (12 mL) and 4C (1.20 g, 3.5 mmol) were added to a 100 mL reaction flask and cooled to 0° C. 2-[(aminooxy)sulfonyl]-1,3,5-trimethylbenzene (1E) (1.14 g, 5.3 mmol) was added in portions, and then sodium bicarbonate (0.59 g, 7.0 mmol) was added to the reaction. Upon completion of the addition, the mixture was reacted at room temperature for 2 h. After that, potassium carbonate (0.97 g, 7.0 mmol) was added to the reaction and reacted at room temperature overnight. Water (150 mL) was added to the reaction liquid. The aqueous phase was extracted with ethyl acetate (200 mL×2). The organic phases were combined, washed with saturated sodium chloride (100 mL), dried over anhydrous sodium sulphate, filtered and concentrated to obtain a crude product (2.0 g) of 4D.
LCMS m/z=357.2[M+1]+.
Step 4:
The crude product (2.0 g, about 5.6 mmol) of 4D obtained in the previous step was dissolved in methanol (10.0 mL) and acetic acid (2.0 mL), and the resulting solution was cooled to 5° C.-10° C. After that, an aqueous solution (2.0 mL) of sodium nitrite (0.77 g, 11.2 mmol) was added dropwise to the reaction liquid. Upon completion of the addition, the resulting mixture was reacted at room temperature for 16 h. Water (20 mL) was added dropwise to the reaction liquid. Upon completion of the addition, filtration was performed. The filter cake was washed with water (5 mL×2) and dried with suction to obtain a crude product (2.00 g) of 4E.
Step 5:
The crude product (2.00 g, about 5.2 mmol) of 4E obtained in the previous step was dissolved in ethanol (10 mL) and water (10 mL). After that, ammonium chloride (2.78 g, 52.0 mmol) and iron powder (1.46 g, 26.0 mmol) were added to the reaction, reacted at 65° C. for 2 h and filtered. The filtrate was extracted with EA (10 mL×3). The organic phases were combined, washed with saturated brine (10 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and then the residue was purified by column chromatography (DCM:MeOH=30:1) to obtain 4F (0.92 g, yield: 48%).
LCMS m/z=372.3[M+1]+.
Step 6:
4F (0.92 g, 2.5 mmol), 2-chloro-4-(4-fluorophenyl)thiazole-5-carbonitrile (1I) (0.72 g, 3.0 mmol), 2,6-dimethylpyridine (0.40 g, 3.8 mmol) and N,N-dimethylacetamide (5 mL) were added, and the resulting mixture was warmed to 70° C. and reacted overnight. The reaction was quenched by adding a 10% sodium chloride solution (20 mL) and extracted with ethyl acetate (20 mL×3). The organic phases were combined, washed with saturated brine (20 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and then the residue was subjected to column chromatography (DCM:MeOH (v/v)=30:1) to obtain 4G (1.00 g, yield: 70%).
LCMS m/z=574.3[M+1]+.
Step 7:
4G (15.50 g) was dissolved in tetrahydrofuran (10 mL) and cooled to 0° C. under nitrogen protection. Sodium hydride (0.10 g, 2.1 mmol, 60 wt %) was added in portions. Upon completion of the addition, the mixture was reacted under such condition for 10 min, and then iodomethane (0.16 mL, 2.1 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was naturally warmed to room temperature and reacted for 1 h. The reaction was quenched by adding 10 mL of methanol, and the reaction liquid was subjected to rotary evaporation to obtain a crude product (1.20 g) of 4H, which was directly used in the next reaction without purification.
LCMS m/z=588.3[M+1]+.
Compound 4H may also be synthesized via the following method.
Raw materials 4J (see WO 2019228403 A1 for synthesis of intermediate 3) (948 mg, 2.0 mmol), N-Boc-piperazine-d8 (580 mg, 3.0 mmol), tris(dibenzylideneacetone)dipalladium (183 mg, 0.2 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (230 mg, 0.4 mmol) and sodium tert-butoxide (576 mg, 6.0 mmol) were added to dry 1,4-dioxane (20 mL) at room temperature. The system was subjected to nitrogen replacement three times, warmed to 105° C. and reacted for 2 h. The resultant was cooled to room temperature, subjected to rotary evaporation and directly purified by column chromatography (PE:EA=5:1-2:1) to obtain 4H as a brown foamy solid (530 mg, yield: 45%).
LCMS m/z=588.3[M+1]+.
Trifluoroacetic acid (5.0 mL) was dropwise added to a solution of compound 4H (1.20 g, crude, obtained in the previous step) in dichloromethane (15.0 mL) at 0° C. under nitrogen protection, and then the resulting mixture was naturally warmed to room temperature and stirred for another 2 h. After the reaction liquid was subjected to rotary evaporation, 10 mL of dichloromethane was added, and then the resulting mixture was neutralized to pH=8 with saturated sodium carbonate, extracted with dichloromethane (10 mL×2), washed with water (10 mL×1), washed with saturated sodium chloride (10 mL×1), dried over anhydrous sodium sulphate, filtered and concentrated under reduced pressure to obtain a crude product (0.90 g) of 4I, which was directly used in the next reaction without purification.
LCMS m/z=488.3[M+1]+.
Step 9:
The crude product (860 mg, about 1.8 mmol) of compound 4I, 2-chloro-1-(3-hydroxyacridin-1-yl)ethan-1-one (296 mg, 2.0 mmol) and potassium carbonate (500 mg, 3.6 mmol) were added to acetonitrile (50 mL) at room temperature, and then the resulting mixture was warmed to 80° C. and reacted for 5 h. The reaction product was cooled to room temperature and then filtered by suction. The filter cake was washed with dichloromethane (30 mL×2). The filtrate was concentrated, and then the residue was subjected to column chromatography (DCM:MeOH=30:1) to obtain compound 4 (880 mg, yield: 81%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.19-8.11 (m, 2H), 7.20-7.12 (m, 2H), 6.44 (d, 1H), 4.67 (s, 1H), 4.50-4.40 (m, 1H), 4. 32-4.24 (m, 1H), 4.16-4.09 (m, 1H), 3.94-3.86 (m, 1H), 3.58 (s, 3H), 3.24-3.06 (m, 2H), 2.72 (q, 2H), 2.59 (s, 1H), 1.32 (t, 3H).
LCMS m/z=601.2[M+1]+.
Step 1:
Tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3E) (2.8 g, 5.0 mmol) was dissolved in tetrahydrofuran (30 mL) and cooled to 0° C. under nitrogen protection. Sodium hydride (0.24 g, 6.0 mmol, 60 wt %) was added in portions. Upon completion of the addition, the resulting mixture was reacted under such condition for 10 min, and then methyl iodide (1.42 g, 10 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (50 mL) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain a crude product of the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (5A) as a yellow solid (2.8 g).
LCMS m/z=580.3[M+1]+.
Step 2:
The crude product (2.8 g) of tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (5A) was dissolved in dichloromethane (30 mL), and trifluoroacetic acid (15 mL) was added dropwise. The resulting mixture was reacted for about 3 h and then concentrated under reduced pressure. Dichloromethane (100 mL) was added to the residue, and the pH was adjusted to 8 with saturated sodium bicarbonate. Liquid separation was performed. The aqueous phase was extracted with dichloromethane (100 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound 2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (5B) as a yellow solid (2.0 g, two-step yield: 83.3%).
LCMS m/z=480.1[M+1]+.
Step 3:
2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (0.31 g, 0.65 mmol) was dissolved in acetonitrile (10 mL). 2-chloro-1-(3-hydroxyazetidin-1-yl-3-d)ethan-1-one (intermediate 2) (0.15 g, 1.0 mmol) and potassium carbonate (0.27 g, 2.0 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 6 h. The reaction was quenched by adding water (50 mL) and extracted with dichloromethane (50 mL×3). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl-3-d)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 5) (90 mg, yield: 23%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.18-8.10 (m, 2H), 7.20-7.10 (m, 2H), 6.45 (d, 1H), 4.43 (d, 1H), 4.25 (d, 1H), 4.11 (dd, 1H), 3.89 (d, 1H), 3.58 (s, 3H), 3.28-3.06 (m, 7H), 2.81-2.66 (m, 6H), 1.31 (t, 3H).
LCMS m/z=594.2[M+1]+.
Step 1:
Potassium carbonate (0.14 g, 1 mmol) and ethyl 2-bromoacetate-d2 (intermediate 3) (0.17 g, 1 mmol) were successively added to a solution of 2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (5B) (0.31 g, 0.65 mmol) in acetonitrile (10 mL), and the mixture was stirred at room temperature for 1 h. After the reaction was completed, the mixture was diluted with saturated brine (30 mL) and extracted with ethyl acetate (30 mL×2). The organic phases were dried over anhydrous sodium sulphate, filtered and concentrated. The residue was purified by column chromatography on silica gel (ethyl acetate:petroleum ether=1:1) to obtain ethyl 2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetate-d2 (6A) as a pale yellow solid (0.32 g, 86.7%).
LC-MS(ESI): m/z=568.2[M+H]+.
Step 2:
Ethyl 2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetate-d2 (6A) (0.32 g, 0.56 mmol) was dissolved in tetrahydrofuran (6 mL), and methanol (2 mL) and water (2 mL) were added. The mixture was stirred uniformly, and then lithium hydroxide monohydrate (84 mg, 2 mmol) was added. The resulting mixture was reacted at room temperature for 1 h, adjusted to pH 5 with hydrochloric acid (1N aqueous solution) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain 2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetic-2,2-d2 acid (6B) as a pale yellow solid (0.24 g, yield: 79%).
LCMS m/z=540.2 [M+1]+.
Step 3:
2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetic-2,2-d2 acid (6B) (0.24 g, 0.45 mmol) was dissolved in N,N-dimethylformamide (5 mL). Triethylamine (0.15 g, 1.5 mmol) and HATU (0.19 g, 0.5mmol) were successively added, and the resulting mixture was stirred for 10 min. After that, a solution of 4-hydroxyazetidine hydrochloride (0.7 mmol, 76 mg) in N,N-dimethylformamide (5 mL) was added, and the resulting mixture was reacted at room temperature for 1 h. The reaction was quenched by adding water (30 mL) and extracted with dichloromethane (50 mL×3). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl-1,1-d2)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 6) (0.12 g, yield: 45%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.19-8.10 (m, 2H), 7.20-7.12 (m, 2H), 6.45 (d, 1H), 4.67 (s, 1H), 4.49-4.40 (m, 1H), 4.30-4.24 (m, 1H), 4.16-4.08 (m, 1H), 3.96-3.87 (m, 1H), 3.58 (s, 3H), 3.26 (s, 4H), 2.84 (s, 4H), 2.72 (q, 2H), 2.59 (s, 1H), 1.32 (t, 3H).
LCMS m/z=595.3[M+1]+.
The crude product (300 mg) of 2-((2-ethyl-6-fluoro-5-(piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (3G) was dissolved in acetonitrile (5 mL). Intermediate 2 (103 mg, 0.7 mmol) and potassium carbonate (172 mg, 1.3 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 6 h. The reaction was quenched by adding water (10 mL) and extracted with ethyl acetate (10 mL×3). The organic phases were combined, washed with saturated brine (10 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol (v/v)=20:1) to obtain compound 7 as a white solid (200 mg, 54%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.17-8.13 (m, 2H), 7.18-7.14 (m, 2H), 6.45 (d, 1H), 4.46-4.44 (m, 1H), 4.29-4.26 (m, 1H), 4.14-4.10 (m, 1H), 3.92-3.89 (m, 1H), 3.26-3.15 (m, 6H), 2.83(s, 4H), 2.75-2.69 (m, 2H), 2.54(s, 1H), 1.32 (t, 3H).
LCMS m/z=597.3[M+1]+.
Step 1:
Potassium carbonate (0.21 g, 1.5 mmol) and ethyl 2-bromoacetate-d2 (intermediate 3) (0.17 g, 1 mmol) were added successively to a solution of compound 3G (0.35 g, 0.73 mmol) in acetonitrile (10 mL), and the mixture was stirred at room temperature for 1 h. After the reaction was completed, the mixture was diluted with saturated brine (30 mL) and extracted with ethyl acetate (30 mL×2). The organic phases were dried over anhydrous sodium sulphate, filtered and concentrated. The residue was purified by column chromatography on silica gel (ethyl acetate:petroleum ether=1:1) to obtain a pale yellow solid (0.38 g, yield: 92%).
Step 2:
Ethyl 2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetate-d2 (8A) (0.38 g, 0.67 mmol) was dissolved in tetrahydrofuran (9 mL), and methanol (3 mL) and water (3 mL) were added. The mixture was stirred uniformly, and then lithium hydroxide monohydrate (126 mg, 3 mmol) was added. The resulting mixture was reacted at room temperature for 1 h, adjusted to pH 5 with hydrochloric acid (IN aqueous solution) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, dried over anhydrous sodium sulphate and concentrated to obtain 2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetic-2,2-d2 acid (8B) as a pale yellow solid (0.32 g, yield: 88%).
Step 3:
2-(4-(3-((5-cyano-4-(4-fluorophenyl)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazin-1-yl)acetic-2,2-d2 acid (8B) (0.32 g, 0.59 mmol) was dissolved in N,N-dimethylformamide (10 mL). Triethylamine (0.20 g, 2.0 mmol) and HATU (0.27 g, 0.7 mmol) were successively added, and the mixture was stirred for 10 min. After that, a solution of 4-hydroxyazetidine hydrochloride (1 mmol, 0.11 g) in N,N-dimethylformamide (5 mL) was added, and the resulting mixture was reacted at room temperature for 1 h. The reaction was quenched by adding water (30 mL) and extracted with dichloromethane (50 mL×3). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl-1,1-d2)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound 8) (0.28 g, 79%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 8.19-8.11 (m, 2H), 7.20-7.11 (m, 2H), 6.44 (d, 1H), 4.67 (s, 1H), 4.49-4.41 (m, 1H), 4.32-4.24 (m, 1H), 4.15-4.08 (m, 1H), 3.94-3.84 (m, 1H), 3.23 (s, 4H), 2.79-2.66 (m, 6H), 2.62 (s, 1H), 1.32 (t, 3H).
LCMS m/z=598.2[M+1]+.
Step 1:
Tert-butyl 4-(3-amino-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (3D) (1.02 g, 2.8 mmol), 2-chloro-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (intermediate 4) (0.68 g, 2.8 mmol) and 2,6-dimethylpyridine (0.60 g, 5.6 mmol) were successively added to N,N-dimethylacetamide (20 mL), and the resulting mixture was warmed to 70° C. and reacted for 5 h. The reaction was quenched by adding a 10% sodium chloride solution (60 mL) and extracted with ethyl acetate (60 mL×3). The organic phases were combined, washed with saturated brine (60 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (petroleum ether:ethyl acetate (v/v)=3:1) to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (9A) as a yellowish-brown solid (1.02 g, yield: 64%).
LCMS m/z=570.2[M+1]+.
Step 2:
Tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (9A) (0.51 g, 0.89 mmol) was dissolved in N,N-dimethylformamide (10 mL). Potassium carbonate (0.27 g, 2 mmol) was added. Methyl iodide (0.19 g, 1.34 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (50 mL) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (9B) as a yellow solid (0.52 g, yield: 100%).
LCMS m/z=584.2[M+1]+.
Step 3:
The compound (3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)(methyl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (9B) (0.52 g, 0.89 mmol) was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (3 mL) was added dropwise. The resulting mixture was reacted for about 2 h and then concentrated under reduced pressure to obtain a trifluoroacetate (0.58 g, a brown viscous substance) of the target compound 9C, and the crude product was directly used in the next reaction.
LCMS m/z=484.2[M+1]+.
Step 4:
The trifluoroacetate (0.58 g) of the target compound 9C was dissolved in acetonitrile (15 mL). Potassium carbonate (0.41 g, 3 mmol) and 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone (1M) (0.22 g, 1.5 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 3 h. The reaction was quenched by adding water (50 mL) and extracted with ethyl acetate (50 mL×3). The organic phases were combined, washed with saturated brine (50 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol=20:1) to obtain the title compound 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl)amino)-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (compound 9) (0.39 g, two-step yield: 73%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 6.45 (d, 1H), 4.67 (s, 1H), 4.50-4.41 (m, 1H), 4.32-4.24 (m, 1H), 4.15-4.08 (m, 1H), 3.94-3.86 (m, 1H), 3.58 (s, 3H), 3.22 (s, 4H), 3.17-3.10 (m, 2H), 2.78 (s, 4H), 2.72 (q, 2H), 2.65 (s, 1H), 1.32 (t, 3H).
LCMS m/z=597.3[M+1]+.
Step 1:
Tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (9A) (0.51 g, 0.89 mmol) was dissolved in N,N-dimethylformamide (10 mL). Potassium carbonate (0.27 g, 2 mmol) was added, and methyl-d3 iodide (0.19 g, 1.34 mmol) was added dropwise. Upon completion of the addition, the resulting mixture was reacted at room temperature for 30 min. The reaction was quenched by adding water (50 mL) and extracted with ethyl acetate (50 mL×2). The organic phases were combined, washed with saturated brine (100 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure to obtain the target compound tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (10A) as a yellow solid (0.51 g, yield: 98%).
LCMS m/z=587.2[M+1]+.
Step 2:
Tert-butyl 4-(3-((5-cyano-4-(4-fluorophenyl-2,3,5,6-d4)thiazol-2-yl)(methyl-d3)amino)-2-ethyl-6-fluoropyrazolo[1,5-a]pyridin-5-yl)piperazine-1-carboxylate (10A) (0.51 g, 0.88 mmol) was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (3 mL) was added dropwise. The resulting mixture was reacted for about 2 h and then concentrated under reduced pressure to obtain a trifluoroacetate (0.62 g, a brown viscous substance) of the target compound 10B, and the crude product was directly used in the next reaction.
LCMS m/z=487.3[M+1]+.
Step 3:
The trifluoroacetate (0.62 g) of compound 10B was dissolved in acetonitrile (15 mL). Potassium carbonate (0.46 g, 3.3 mmol) and 2-chloro-1-(3-hydroxyazetidin-1-yl)ethanone (1M) (0.22 g, 1.5 mmol) were successively added, and the resulting mixture was warmed to 80° C. and reacted for 4 h. The reaction was quenched by adding water (60 mL) and extracted with ethyl acetate (60 mL×3). The organic phases were combined, washed with saturated brine (60 mL×1), dried over anhydrous sodium sulphate and filtered. The filtrate was concentrated under reduced pressure, and the residue was separated by column chromatography on silica gel (dichloromethane/methanol (v/v)=20:1) to obtain the title compound 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl-2,3,5,6-d4)thiazole-5-carbonitrile (compound 10) (0.35 g, two-step yield: 66%).
1H NMR (400 MHZ, CDCl3) δ 8.27 (d, 1H), 6.44 (d, 1H), 4.66 (s, 1H), 4.50-4.40 (m, 1H), 4.30-4.22 (m, 1H), 4.15-4.05 (m, 1H), 3.93-3.85 (m, 1H), 3.21 (s, 4H), 3.18-3.04 (m, 2H), 2.90 (s, 1H), 2.77-2.66 (m, 6H), 1.32 (t, 3H).
LCMS m/z=600.3[M+1]+.
A free base (9.1 g, 15.3 mmol) of 2-((2-ethyl-6-fluoro-5-(4-(2-(3-hydroxyazetidin-1-yl)-2-oxoethyl)piperazin-1-yl)pyrazolo[1,5-a]pyridin-3-yl)(methyl-d3)amino)-4-(4-fluorophenyl)thiazole-5-carbonitrile (compound A, i.e., compound 3) prepared in example 3 was added to ethanol (50 mL), warmed to 75° C., stirred for 2 h and then dissolved. The mixture was gradually cooled to room temperature under slow stirring, with a large amount of white solids precipitated out, and filtered by suction. The filter cake was washed with ethanol (10 mL), and then dried under vacuum at 40° C. for 24 h to obtain a crystal form I of compound A as a white solid (6.2 g, yield: 68%). The XRPD pattern thereof was as shown in
The dynamic solubility of the crystal form I of compound A (example 11) in water and three biological vehicles was evaluated. The dynamic solubility (1 h, 2 h, 4 h and 24 h) of samples in four solvent systems, i.e., water, SGF, FaSSIF and FeSSIF, was measured by means of rotational blend (25 rpm) at a feeding concentration of 5-10 mg/mL at 37° C. The samples at each time point were centrifuged (12000 rpm, 5 min) and filtered (0.45 μm PTFE filter), and the HPLC concentration and pH value of the filtrates were measured. The solubility test results were shown in Table 2.
The initial pH values of H2O, SGF, FaSSIF and FeSSIF were 6.9, 1.8, 6.5 and 5.0, respectively.
Preparation of simulated gastric fluid (SGF): 0.1 g of NaCl and 0.05 g of Triton X-100 were weighted to a 50 mL volumetric flask, and purified water was added to obtain a clear solution. 67.5 μL of concentrated hydrochloric acid (12 M) was added, and the pH was adjusted to 1.8 with 1 M hydrochloric acid or a 1 M NaOH solution. Purified water was added to a constant volume.
Preparation of simulated intestinal fluid (FaSSIF) in a fasted state: 0.17 g of anhydrous NaH2PO4, 0.021 g of NaOH and 0.31 g of NaCl were weighed to a 50 mL volumetric flask. About 48 mL of purified water was added to obtain a clear solution, and the pH was adjusted to 6.5 with 1 M hydrochloric acid or a 1 M NaOH solution. Purified water was added to a constant volume, and 0.11 g of SIF powder was weighed to obtain a clear solution.
Preparation of simulated intestinal fluid (FeSSIF) in a feeding state: 0.41 mL of glacial acetic acid, 0.20 g of NaOH and 0.59 g of NaCl were weighed to a 50 mL volumetric flask. About 48 mL of purified water was added to obtain a clear solution, and the pH was adjusted to 5.0 with 1 M hydrochloric acid or a 1 M NaOH solution. Purified water was added to a constant volume, and 0.56 g of SIF powder was weighed to obtain a clear solution.
The crystal form I (example 11) was placed at 25° C./60% RH and 40° C./75% RH for 12 days; and then the sample purity was detected, and the crystal form change was observed. The results were as shown in Table 3.
Autocrine motility factor is a phosphodiesterase in plasma that converts lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA). Therefore, the formation of LPA can be used to evaluate the potency of an autocrine motility factor inhibitor. The potency of the compound in mixed ex vivo human plasma was evaluated.
Whole blood was anticoagulated with heparin and centrifuged, and then plasma was collected. 5 μL of gradient-diluted test compounds or DMSO was added to 95 μL of plasma and incubated at 37° C. for 2 h. After that, a stop buffer (40 mM disodium hydrogen phosphate buffer, containing 30 mM of citric acid, pH=4) was added. LPA in plasma was detected by LC-MSMS before and after incubation. In order to determine the concentration of LPA 18:2 or LPA 20:4 in the plasma in study, calibration standard substances at 20000 ng/mL, 10000 ng/mL, 5000 ng/ml, 2000 ng/ml, 1000 ng/mL, 500 ng/mL, 200 ng/ml, 100 ng/mL, 50 ng/ml, 20 ng/mL and 10 ng/ml for LPA 18:2 or LPA 20:4 were prepared by performing serial dilution in butanol. 3 μL of calibration standard solution was added to 27.0 μL of blank plasmas in each 1.5 mL microcentrifuge tube to produce 1× calibration standard substances. 30.0 μL of standard substances or plasma in study was added to each 1.5 mL microcentrifuge tube. 200 μL of butanol (containing 25.0 ng/mL LPA 17:0 for internal control) was added to each 1.5 mL microcentrifuge tube containing the plasma in study or calibration standard substance. Vortex oscillation was performed for 1 min, and centrifugation was performed at 10000 rpm for 10 min. 180 μL of the supernatant was transferred to a 96-well plate, and LC/MS/MS was used together with the standard substance to quantify the concentration of LPA 18:2 in the plasma. In short, 8 μL of the solution was injected, and LC-MS/MS analysis using an ACQUITY UPLC BEH C18 column (2.1×50 mm, 1.7 μm) was performed, wherein mobile phase A [20 mM NH4OAC aqueous solution (0.1% FA)] and mobile phase B [5 mM NH4OAC aqueous solution/0.2% FA in ACN=5:95] were used. The parameters of a mass spectrometer for LPA 18:2 were optimized by means of deprotonation of molecular ions. For LPA 18:2 at m/z 433.2([M−H]−), LPA 20:4 at 457.2, and LPA 18:2 and LPA 20:4 both at m/z 152.8, abundant product ions were obtained. Quantitative data were obtained in a multiple reaction monitoring (MRM) negative electrospray ionization mode.
The inhibition rates of compounds at different concentrations on LPA formation were determined by comparing the changes of LPA levels in incubated and unincubated plasma. The IC50 of the compounds was calculated as a calculation inhibition rate, and the relative concentration at each well=the concentration at each well−the average concentration at baseline.
Inhibition rate %=(average relative concentration at control well−average relative concentration at test well)/average relative concentration at control well×100%. The curve was plotted with the inhibition rates as a y axis and the compound concentrations as an x axis, and fitting was performed by GraphPad Prism7.0 using log (inhibitor) and normalized response (variable slope).
Test objective: test compounds were given to SD rats via single-dose intravenous and intragastric administration, the concentrations of the test compounds in plasma of rats were measured, and the pharmacokinetic characteristics and bioavailability of test compounds in rats were evaluated.
Test subject: the compound in example 3 of the present invention.
Test animals: male SD rats, about 220 g, 6-8 weeks old, six rats/compound, purchased from Chengdu Ddossy Experimental Animals Co., Ltd.
Test method: on the day of the test, 6 SD rats were randomly grouped according to their body weights. The rats were fasted with water available for 12-14 h one day before the administration, and were fed 4 h after the administration. The administration was performed according to Table 5.
Before and after the administration, 0.1 mL of blood was taken from the orbits of the rats under isoflurane anaesthesia, and placed in an EDTAK2 centrifuge tube. Centrifugation was performed at 5000 rpm at 4° C. for 10 min, and plasma was collected.
Time points for sample collection in group G1 comprise 0 min, 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h and 24 h.
Time points for sample collection in group G2 comprise 0 min, 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h and 24 h.
Before analysis and detection, all samples were stored at −80° C.
30 μL of each of plasma samples, standard curve samples and quality control samples was taken, and 200 μL of acetonitrile solution containing an internal standard was added, and the resulting mixture was homogeneously mixed by vortex and centrifuged at 4° C. at 12000 rpm for 10 min. 170 μL of the supernatant was taken to a 96-well plate, and LC-MS/MS analysis was performed, wherein the sample size was 2 μL.
The main pharmacokinetic parameters were analyzed by a non-compartmental model using WinNonlin 8.0 software. The test results were as shown in Table 6.
Test objective: the pharmacokinetic characteristics and bioavailability of test compounds in mice were evaluated.
Test animals: thirty-six C57 male mice, about 25 g, 6-8 weeks old, purchased from Chengdu Ddossy Experimental Animals Co., Ltd., with the production license number of SCXK (CHUAN) 2020-030.
Test subject: compound I-1 (compound 101 in WO 2019228403 A1) and compound 3.
Test design:
Vehicle for intravenous administration: 5% DMA+5% Solutol+90% Saline; vehicle for intragastric administration: 0.5% MC
Before and after the administration, 0.06 mL of blood was taken from the orbits under isoflurane anaesthesia and placed in an EDTAK2 centrifuge tube. The blood was centrifuged at 5000 rpm at 4° C. for 10 min, and plasma was collected. Time points for blood collection: 0 min, 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 10 h and 24 h. Before analysis and detection, all plasma samples were stored at −80° C. Test results were shown in Table 8.
Test objective: the pharmacokinetic characteristics and bioavailability of test compounds in dogs were evaluated.
Test animals: twelve male beagles, 8-10 kg, 0.5-1.5 years old, purchased from Beijing Marshall Biotechnology Co. Ltd., with the production license number of SCXK (JING) 2016-001.
Test subject: compound I-1 (compound 101 in WO 2019228403 A1) and compound 3.
Test design:
Vehicle for intravenous administration: 5% DMSO+5% Solutol+90% Saline;
Before and after the administration, 1.0 mL of blood was taken from the veins of the forelimbs and placed in an EDTAK2 centrifuge tube. The blood was centrifuged at 5000 rpm at 4° C. for 10 min, and plasma was collected. Time points for blood collection: 0 min, 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h and 24 h. Before analysis and detection, all plasma samples were stored at −80° C. Test results were shown in Table 10.
Test objective: the pharmacokinetic characteristics and bioavailability of test compounds in monkeys were evaluated.
Test animals: twelve male Cynomolgus macaques, 2.4-5.9 kg, 3-5.5 years old, purchased from Suzhou Xishan Zhongke Laboratory Animal Co., Ltd., with the production license number of SCXK (SU) 2018-0001.
Test subject: compound I-1 (compound 101 in WO 2019228403 A1) and compound 3.
Test design:
Vehicle for intravenous administration: 5% DMSO+5% Solutol+90% Saline;
Before and after the administration, 1.0 mL of blood was taken from the veins of the forelimbs and placed in an EDTAK2 centrifuge tube. The blood was centrifuged at 5000 rpm at 4° C. for 10 min, and plasma was collected. Time points for blood collection: 0 min, 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h and 24 h. Before analysis and detection, all plasma samples were stored at −80° C. Test results were shown in Table 12.
2.5 Test of Effects on hERG Potassium Channel
Test compounds: compound I-1 (compound 101 in WO 2019228403 A1) and example compound 3.
The effect of the example compounds on the current of an hERG potassium channel (human Ether-à-go-go Related Gene potassium channel) was tested by 10 means of an electrophysiological manual patch-clamp method.
HEK293 cell lines stably expressing hERG ion channels were purchased from Invitrogen. The cells were cultured in a culture medium containing 85% DMEM, 10% dialyzed fetal bovine serum, a 0.1 mM MEM non-essential amino acid solution, a 100 U/mL penicillin-streptomycin solution, 25 mM HEPES, 5 μg/mL blasticidin and 400 μg/mL geneticin (G418). When the cell density was increased to 40%-80% of the bottom area of the culture dish, the cells were digested with trypsin and passaged three times a week. Before the test, the cells were cultured in a 6 cm culture dish at a density of 5×105, induced by adding 1 μg/mL doxycycline for 48 h, then digested and inoculated on slides for use in the subsequent manual patch-clamp testing.
Extracellular fluid (in mM): 132 mM sodium chloride, 4 mM potassium chloride, 3 mM calcium chloride, 0.5 mM magnesium chloride, 11.1 mM glucose, and 10 mM HEPES (the pH was adjusted to 7.35 by using sodium hydroxide).
A test compound was firstly dissolved in DMSO and prepared into a stock solution with a final concentration of 30 mM. The original stock solution was then diluted with DMSO in a certain ratio to form four gradient-dilution solutions with the concentrations of 10 mM, 3.33 mM, 1.11 mM and 0.37 mM, respectively. Before the beginning of the test, the gradient-dilution solutions of the test compound were diluted with the extracellular fluid to form working solutions with a series of gradient concentrations according to 1:1000, and the final concentrations of the working solutions were 30 μM, 10 μM, 3.33 μM, 1.11 μM and 0.37 μM, respectively. Five working solutions with different gradient concentrations were used to determine the potential inhibitory effect of the compound on the hERG potassium channel and used to fit a dose-response curve and calculate IC50.
Data analysis: The percentage of current inhibition was calculated by the following formula. Data were output by PatchMaster or Clampex10.2 software. The dose-response curve was fitted by Graphpad Prism 8.0 software, and the IC50 value was calculated. Test results were shown in Table 11.
The widely accepted and used criteria for evaluating whether a compound has an inhibitory effect on hERG potassium channels were as follows: non-significant inhibitory effect: IC50>10 μM; medium-level inhibitory effect: 1 μM<IC50<10 μM; and significant inhibitory effect: IC50<1 μM. As can be seen from the test results, compound 3 exhibited a non-significant inhibitory effect on hERG potassium channels.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202011213775.9 | Nov 2020 | CN | national |
| 202011562922.3 | Dec 2020 | CN | national |
| 202110085643.0 | Jan 2021 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2021/128735 | 11/4/2021 | WO |
